Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002203

Physical Location & Seq

Genomic Coordinates Forward (+)

3083869 .. 3089312

Gene Structure 5444 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1044500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGTCTATGGTTGATGAACCGCTATACCCAATAGCCGTTCTCATAGACGAGCTTAAAAATGACGATATCCAGCTGCGGTTGAACTCAATCCGCAGGCTATCTACAATTGCACGTGCTCTTGGGGAAGAGCGAACTCGTAAGGAATTAATCCCCTTTCTGAGCGAGAACAATGACGATGACGATGAGGTACTTCTTGCAATGGCGGAAGAGTTGGGGGTCTTCATTCCATATGTTGGGGGAGTCGAGCATGCGCATGTTTTGCTCCCGCCCCTGGAGACCCTTTGCACTGTTGAGGAAACCTGTGTGAGGGACAAGGCTGTGGAGTCGTTATGTAGGATTGGGTCTCAGATGAGGGAAGGTGATTTGGTCAACTGGTTTATTCCTCTTGTGAAGAGGTTGGCAGCTGGTGAATGGTTTACAGCTCGAGTCTCTGCATGTGGGCTTTTTCATATTGCCTACCCTAGTGCACTGGATACATTGAAGACAGAACTGCGATCAATTTACAGTCAGTTGTGTCAAGATGACATGCCTATGGTTAGGAGGTCTGCTGCTACAAACCTAGGAAAATTTGCAGCAACTGTTGAACCCGCTCATCTGAAGGCTGATATCATGTCTATATTTGAGGATCTTACTCAAGATGATCAAGATTCTGTTCGACTCTTGGCTGTTGAGGGCTGTGCTGCTCTTGGGAAGTTGTTGGAGCCCCAAGATTGTGTTGCACATATCCTCCCCGTTATTGTTAACTTCTCTCAGGATAAGTCTTGGCGTGTGCGTTACATGGTTGCAAATCAACTATATGAGCTTTGTGAAGCTGTGGGGCCTGAGCCTACGAGGACGGACCTGGTTCCGGCATATGTGCGATTGCTTCGAGATAATGAGGCTGAAGTTCGTATAGCAGCTGCTGGCAAAGTTACCAAATTTTGCCGGATTTTAAGTCCGGAACTTGCAATTCAGCATATTCTTCCATGTGTGAAGGAGCTGTCATCAGATTCTTCCCAACATGTCCGGTCTGCTTTGGCTTCAGTTATAATGGGAATGGCTCTTGTATTAGGAAAGGATGCTACAATTGAGCAGCTCCTTCCCATTTTTCTTTCACTCCTGAAGGATGAATTTCCTGATGTGCGTCTTAATATCATTAGCAAGCTTGATCAAGTGAATCAGGTTATTGGAATCGATTTGCTGTCTCAATCCTTGTTACCAGCCATAGTGGAGCTAGCAGAGGATAGACATTGGAGGGTTCGACTTGCAATAATAGAGTACATACCTTTATTGGCAAGTCAGCTGGGTGTAGGGTTCTTTGATGATAAGCTTGGTGCCCTTTGCATGCAGTGGTTACAAGACAAGGTCTACTCAATTCGTGATGCTGCTGCTAATAATTTAAAGCGTCTGGCAGAAGAGTTCGGTCCAGAATGGGCAATGCAACATATTGTTCCACAGGTCCTTCAGATGATAGACAATCCACACTATTTGTATCGAATGACAATTCTTCGTGCAATCTGTCTCCTTGCCCCGGTTATGGGTCCAGAAATCACATGTTCAAAACTGCTGCCAGTGCTCATCAATGCGACAAAGGACAGAGTACCGAACATCAAATTCAATGTCGCAAAGGTGCTGCAGTCCCTTATCCCCATAGTTGATCAGTCTGTGGTGGAGAAAACAATTCGTCCCTCTTTGGTTGAGCTCAGCGAGGACCCAGATGTTGATGTCCGGTTTTTCGCTAACCAGGCTCTTCAGGCAATTGATCATGTCATGATGTCAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0019220 GO:0019222 GO:0031323 GO:0042221 GO:0042325 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050789 GO:0050794 GO:0050896 GO:0051174 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04111 ko04113 ko04114 ko04151 ko04152 ko04261 ko04350 ko04390 ko04391 ko04530 ko04728 ko04730 ko05142 ko05160 ko05165 map03015 map04071 map04111 map04113 map04114 map04151 map04152 map04261 map04350 map04390 map04391 map04530 map04728 map04730 map05142 map05160 map05165

Pfam Domains

Protein Families

HEAT HEAT_2

Protein Analysis

588

Amino Acids

65.63

Weight (kDa)

4.99

Isoelectric Point (pI)

47.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VPS15-like_hel	PF22956	197 - 309	1.2e-07	VPS15-like, helical domain
PPP2R1A-like_HEAT	PF22646	276 - 351	5.8e-29	PPP2R1A-like HEAT repeat
HEAT_2	PF13646	361 - 460	9.5e-06	HEAT repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13320 AT1G13320 AT1G13320 AT1G25490 AT3G25800 AT3G25800 AT3G25800
fragaria_vesca	FvH4_4g27700 FvH4_4g27700 FvH4_5g12331 FvH4_5g12331
malus_domestica	MD06G1214700.v1.1 MD13G1044500.v1.1 MD14G1225600.v1.1 MD16G1045400.v1.1
prunus_persica	Prupe.1G307900_v2.0.a1 Prupe.1G307900_v2.0.a1 Prupe.5G220100_v2.0.a1
pyrus_communis	pycom06g19130 pycom13g03910 pycom14g18680 pycom16g03990
rosa_chinensis	RchiOBHm_Chr4g0435561 RchiOBHm_Chr7g0185211
rosa_laevigata	RLG00000006582
rosa_multiflora	Rmu_sc0001940.1_g000038
rosa_roxburghii	Rroxscaffold_3G00269150 Rroxscaffold_5G00376790
rosa_rugosa	Rorug04G0286500 Rorug04G0286500 Rorug04G0286600 Rorug06G0466800 Rorug06G0466900
rosa_samantha	Rh4AG342000 Rh4BG350200 Rh4CG364900 Rh4DG344500 Rh7AG076000 Rh7BG071300 Rh7CG072600 Rh7DG072000
rosa_wichuraiana	Rw4G029830 Rw7G005990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1028
Acc16I	TGCGCA	1 cut(s)	252
AccB1I	GGYRCC	1 cut(s)	1313
AccB7I	CCANNNNNTGG	1 cut(s)	233
AccBSI	CCGCTC	1 cut(s)	590
AccI	GTMKAC	1 cut(s)	1347
AccIII	TCCGGA	1 cut(s)	937
AciI	CCGC	6 cut(s)	20, 76, 91, 203, 266, 588
AclWI	GGATC	1 cut(s)	633
AcsI	RAATTY	4 cut(s)	566, 917, 1109, 1592
AcuI	CTGAAG	6 cut(s)	617, 903, 1005, 1121, 1427, 1715
AcvI	CACGTG	1 cut(s)	113
AfaI	GTAC	3 cut(s)	189, 1259, 1581
AfiI	CCNNNNNNNGG	7 cut(s)	233, 271, 306, 847, 1510, 1516, 1517
AflIII	ACRYGT	2 cut(s)	1000, 1532
AgsI	TTSAA	5 cut(s)	82, 481, 584, 1539, 1597
AjnI	CCWGG	3 cut(s)	270, 840, 1722
Alw21I	GWGCWC	4 cut(s)	118, 469, 1557, 1683
Alw26I	GTCTC	5 cut(s)	269, 348, 433, 1188, 1505
Alw44I	GTGCAC	1 cut(s)	465
AlwI	GGATC	1 cut(s)	633
AlwNI	CAGNNNCTG	2 cut(s)	578, 902
Ama87I	CYCGRG	1 cut(s)	423
Aor13HI	TCCGGA	1 cut(s)	937
AoxI	GGCC	1 cut(s)	818
ApaLI	GTGCAC	1 cut(s)	465
ApoI	RAATTY	4 cut(s)	566, 917, 1109, 1592
ArsI	GACNNNNNNTTYG	2 cut(s)	1584, 1616
AseI	ATTAAT	1 cut(s)	146
AspA2I	CCTAGG	1 cut(s)	559
AspLEI	GCGC	1 cut(s)	253
AspS9I	GGNCC	6 cut(s)	818, 838, 1403, 1438, 1520, 1690
AsuC2I	CCSGG	1 cut(s)	1511
AsuHPI	GGTGA	2 cut(s)	371, 419
AsuNHI	GCTAGC	1 cut(s)	1213
AvaI	CYCGRG	1 cut(s)	423
AvaII	GGWCC	5 cut(s)	838, 1403, 1438, 1520, 1690
AvrII	CCTAGG	1 cut(s)	559
BaeGI	GKGCMC	2 cut(s)	469, 1318
BanI	GGYRCC	1 cut(s)	1313
BanII	GRGCYC	2 cut(s)	705, 1683
BbrPI	CACGTG	1 cut(s)	113
BbsI	GAAGAC	2 cut(s)	211, 488
Bbv12I	GWGCWC	4 cut(s)	118, 469, 1557, 1683
BceAI	ACGGC	1 cut(s)	20
BciT130I	CCWGG	3 cut(s)	272, 842, 1724
BciVI	GTATCC	1 cut(s)	466
BclI	TGATCA	4 cut(s)	640, 1147, 1636, 1741
BcnI	CCSGG	1 cut(s)	1511
BcoDI	GTCTC	5 cut(s)	269, 348, 433, 1188, 1505
BfaI	CTAG	4 cut(s)	462, 560, 1214, 1762
BfmI	CTRYAG	1 cut(s)	1613
BfuI	GTATCC	1 cut(s)	466
BlnI	CCTAGG	1 cut(s)	559
BlpI	GCTNAGC	1 cut(s)	1682
Bme1390I	CCNGG	4 cut(s)	272, 842, 1511, 1724
Bme18I	GGWCC	5 cut(s)	838, 1403, 1438, 1520, 1690
BmeT110I	CYCGRG	1 cut(s)	423
BmgT120I	GGNCC	6 cut(s)	818, 838, 1403, 1438, 1520, 1690
BmiI	GGNNCC	6 cut(s)	702, 819, 846, 1315, 1521, 1692
BmrFI	CCNGG	4 cut(s)	272, 842, 1511, 1724
BmsI	GCATC	2 cut(s)	1048, 1351
BmtI	GCTAGC	1 cut(s)	1217
BpiI	GAAGAC	2 cut(s)	211, 488
BpmI	CTGGAG	1 cut(s)	293
Bpu10I	CCTNAGC	1 cut(s)	822
Bpu1102I	GCTNAGC	1 cut(s)	1682
BpuEI	CTTGAG	1 cut(s)	618
BpuMI	CCSGG	1 cut(s)	1511
Bsa29I	ATCGAT	1 cut(s)	1173
BsaAI	YACGTR	1 cut(s)	113
BsaI	GGTCTC	2 cut(s)	269, 348
BsaJI	CCNNGG	3 cut(s)	270, 559, 1509
BsaWI	WCCGGW	3 cut(s)	937, 1005, 1707
BsaXI	ACNNNNNCTCC	2 cut(s)	711, 741
Bsc4I	CCNNNNNNNGG	7 cut(s)	233, 271, 306, 847, 1510, 1516, 1517
Bse1I	ACTGG	3 cut(s)	377, 474, 1550
Bse3DI	GCAATG	2 cut(s)	204, 1422
BseAI	TCCGGA	1 cut(s)	937
BseBI	CCWGG	3 cut(s)	272, 842, 1724
BseCI	ATCGAT	1 cut(s)	1173
BseDI	CCNNGG	3 cut(s)	270, 559, 1509
BseGI	GGATG	2 cut(s)	1063, 1111
BseLI	CCNNNNNNNGG	7 cut(s)	233, 271, 306, 847, 1510, 1516, 1517
BseMI	GCAATG	2 cut(s)	204, 1422
BseMII	CTCAG	5 cut(s)	149, 359, 764, 813, 1696
BseNI	ACTGG	3 cut(s)	377, 474, 1550
BseSI	GKGCMC	2 cut(s)	469, 1318
BseYI	CCCAGC	1 cut(s)	1282
BshFI	GGCC	1 cut(s)	820
BshNI	GGYRCC	1 cut(s)	1313
BshVI	ATCGAT	1 cut(s)	1173
BsiHKAI	GWGCWC	4 cut(s)	118, 469, 1557, 1683
BsiHKCI	CYCGRG	1 cut(s)	423
BsiSI	CCGG	6 cut(s)	848, 925, 938, 1006, 1511, 1708
BslFI	GGGAC	3 cut(s)	323, 1603, 1650
BslI	CCNNNNNNNGG	7 cut(s)	233, 271, 306, 847, 1510, 1516, 1517
BsmAI	GTCTC	5 cut(s)	269, 348, 433, 1188, 1505
BsmFI	GGGAC	3 cut(s)	323, 1603, 1650
BsnI	GGCC	1 cut(s)	820
Bso31I	GGTCTC	2 cut(s)	269, 348
BsoBI	CYCGRG	1 cut(s)	423
Bsp1286I	GDGCHC	6 cut(s)	118, 469, 705, 1318, 1557, 1683
Bsp13I	TCCGGA	1 cut(s)	937
Bsp143I	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
Bsp1720I	GCTNAGC	1 cut(s)	1682
BspACI	CCGC	6 cut(s)	20, 76, 91, 203, 266, 588
BspANI	GGCC	1 cut(s)	820
BspCNI	CTCAG	5 cut(s)	150, 358, 763, 814, 1695
BspDI	ATCGAT	1 cut(s)	1173
BspEI	TCCGGA	1 cut(s)	937
BspHI	TCATGA	1 cut(s)	1749
BspLI	GGNNCC	6 cut(s)	702, 819, 846, 1315, 1521, 1692
BspMAI	CTGCAG	1 cut(s)	1617
BspOI	GCTAGC	1 cut(s)	1217
BspPI	GGATC	1 cut(s)	633
BspQI	GCTCTTC	2 cut(s)	120, 1734
BspT107I	GGYRCC	1 cut(s)	1313
BspTNI	GGTCTC	2 cut(s)	269, 348
BsrBI	CCGCTC	1 cut(s)	590
BsrDI	GCAATG	2 cut(s)	204, 1422
BsrI	ACTGG	3 cut(s)	377, 474, 1550
BssECI	CCNNGG	3 cut(s)	270, 559, 1509
BssMI	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
BssT1I	CCWWGG	1 cut(s)	559
Bst2UI	CCWGG	3 cut(s)	272, 842, 1724
Bst4CI	ACNGT	3 cut(s)	289, 506, 580
Bst6I	CTCTTC	5 cut(s)	120, 201, 386, 1389, 1734
BstAPI	GCANNNNNTGC	1 cut(s)	259
BstBAI	YACGTR	1 cut(s)	113
BstC8I	GCNNGC	6 cut(s)	95, 249, 904, 1142, 1215, 1325
BstDEI	CTNAG	5 cut(s)	158, 345, 750, 822, 1682
BstENI	CCTNNNNNAGG	1 cut(s)	304
BstF5I	GGATG	2 cut(s)	1063, 1111
BstHHI	GCGC	1 cut(s)	253
BstKTI	GATC	6 cut(s)	497, 628, 643, 1150, 1639, 1744
BstMAI	GTCTC	5 cut(s)	269, 348, 433, 1188, 1505
BstMBI	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
BstMWI	GCNNNNNNNGC	4 cut(s)	259, 1552, 1724, 1733
BstNI	CCWGG	3 cut(s)	272, 842, 1724
BstNSI	RCATGY	7 cut(s)	251, 257, 438, 529, 1004, 1327, 1536
BstSCI	CCNGG	4 cut(s)	270, 840, 1509, 1722
BstSFI	CTRYAG	1 cut(s)	1613
BstSLI	GKGCMC	2 cut(s)	469, 1318
BstV2I	GAAGAC	2 cut(s)	211, 488
BstX2I	RGATCY	1 cut(s)	625
BstYI	RGATCY	1 cut(s)	625
Bsu15I	ATCGAT	1 cut(s)	1173
BsuI	GTATCC	1 cut(s)	466
BsuRI	GGCC	1 cut(s)	820
BsuTUI	ATCGAT	1 cut(s)	1173
BtsCI	GGATG	2 cut(s)	1063, 1111
BtsI	GCAGTG	1 cut(s)	1334
BtsIMutI	CAGTG	4 cut(s)	285, 467, 1334, 1557
Cac8I	GCNNGC	6 cut(s)	95, 249, 904, 1142, 1215, 1325
CaiI	CAGNNNCTG	2 cut(s)	578, 902
CciI	TCATGA	1 cut(s)	1749
CfoI	GCGC	1 cut(s)	253
Cfr13I	GGNCC	6 cut(s)	818, 838, 1403, 1438, 1520, 1690
ClaI	ATCGAT	1 cut(s)	1173
CseI	GACGC	2 cut(s)	1112, 1373
CsiI	ACCWGGT	1 cut(s)	840
Csp6I	GTAC	3 cut(s)	188, 1258, 1580
CspCI	CAANNNNNGTGG	2 cut(s)	1449, 1484
CviQI	GTAC	3 cut(s)	188, 1258, 1580
DdeI	CTNAG	5 cut(s)	158, 345, 750, 822, 1682
DpnI	GATC	6 cut(s)	496, 627, 642, 1149, 1638, 1743
DpnII	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
DraI	TTTAAA	1 cut(s)	1380
Eam1104I	CTCTTC	5 cut(s)	120, 201, 386, 1389, 1734
EarI	CTCTTC	5 cut(s)	120, 201, 386, 1389, 1734
EciI	GGCGGA	1 cut(s)	218
Ecl136II	GAGCTC	1 cut(s)	1681
Eco130I	CCWWGG	1 cut(s)	559
Eco24I	GRGCYC	2 cut(s)	705, 1683
Eco31I	GGTCTC	2 cut(s)	269, 348
Eco32I	GATATC	2 cut(s)	67, 607
Eco47I	GGWCC	5 cut(s)	838, 1403, 1438, 1520, 1690
Eco53kI	GAGCTC	1 cut(s)	1681
Eco57I	CTGAAG	6 cut(s)	617, 903, 1005, 1121, 1427, 1715
Eco72I	CACGTG	1 cut(s)	113
Eco88I	CYCGRG	1 cut(s)	423
EcoICRI	GAGCTC	1 cut(s)	1681
EcoNI	CCTNNNNNAGG	1 cut(s)	304
EcoO109I	RGGNCCY	3 cut(s)	818, 1438, 1690
EcoRII	CCWGG	3 cut(s)	270, 840, 1722
EcoRV	GATATC	2 cut(s)	67, 607
EcoT14I	CCWWGG	1 cut(s)	559
EcoT38I	GRGCYC	2 cut(s)	705, 1683
ErhI	CCWWGG	1 cut(s)	559
FaqI	GGGAC	3 cut(s)	323, 1603, 1650
FauI	CCCGC	2 cut(s)	273, 595
FauNDI	CATATG	2 cut(s)	229, 853
FbaI	TGATCA	4 cut(s)	640, 1147, 1636, 1741
FblI	GTMKAC	1 cut(s)	1347
FokI	GGATG	2 cut(s)	1070, 1118
FriOI	GRGCYC	2 cut(s)	705, 1683
FspAI	RTGCGCAY	1 cut(s)	252
FspBI	CTAG	4 cut(s)	462, 560, 1214, 1762
FspI	TGCGCA	1 cut(s)	252
GlaI	GCGC	1 cut(s)	252
GsaI	CCCAGC	1 cut(s)	1286
GsuI	CTGGAG	1 cut(s)	293
HaeIII	GGCC	1 cut(s)	820
HapII	CCGG	6 cut(s)	848, 925, 938, 1006, 1511, 1708
HgaI	GACGC	2 cut(s)	1112, 1373
HhaI	GCGC	1 cut(s)	253
Hin6I	GCGC	1 cut(s)	251
HinP1I	GCGC	1 cut(s)	251
HincII	GTYRAC	2 cut(s)	370, 742
HindII	GTYRAC	2 cut(s)	370, 742
HindIII	AAGCTT	2 cut(s)	1142, 1307
HinfI	GANTC	8 cut(s)	240, 323, 426, 647, 657, 989, 1156, 1170
HpaI	GTTAAC	1 cut(s)	742
HpaII	CCGG	6 cut(s)	848, 925, 938, 1006, 1511, 1708
HphI	GGTGA	2 cut(s)	371, 419
Hpy166II	GTNNAC	5 cut(s)	370, 417, 467, 742, 1348
Hpy188I	TCNGA	5 cut(s)	159, 348, 597, 988, 1446
Hpy8I	GTNNAC	5 cut(s)	370, 417, 467, 742, 1348
HpyAV	CCTTC	6 cut(s)	350, 592, 967, 1088, 1096, 1451
HpyCH4III	ACNGT	3 cut(s)	289, 506, 580
HpyCH4IV	ACGT	1 cut(s)	112
HpyF10VI	GCNNNNNNNGC	4 cut(s)	259, 1552, 1724, 1733
HpyF3I	CTNAG	5 cut(s)	158, 345, 750, 822, 1682
HpySE526I	ACGT	1 cut(s)	112
HspAI	GCGC	1 cut(s)	251
Kpn2I	TCCGGA	1 cut(s)	937
Ksp22I	TGATCA	4 cut(s)	640, 1147, 1636, 1741
KspAI	GTTAAC	1 cut(s)	742
Kzo9I	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
LguI	GCTCTTC	2 cut(s)	120, 1734
LmnI	GCTCC	5 cut(s)	267, 700, 976, 1080, 1210
LweI	GCATC	2 cut(s)	1048, 1351
MabI	ACCWGGT	1 cut(s)	840
MaeI	CTAG	4 cut(s)	462, 560, 1214, 1762
MaeII	ACGT	1 cut(s)	112
MaeIII	GTNAC	4 cut(s)	773, 910, 1194, 1332
MalI	GATC	6 cut(s)	496, 627, 642, 1149, 1638, 1743
MbiI	CCGCTC	1 cut(s)	590
MboI	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
MfeI	CAATTG	3 cut(s)	105, 1065, 1737
MflI	RGATCY	1 cut(s)	625
MhlI	GDGCHC	6 cut(s)	118, 469, 705, 1318, 1557, 1683
MlyI	GAGTC	4 cut(s)	249, 332, 435, 651
MmeI	TCCRAC	1 cut(s)	678
MroI	TCCGGA	1 cut(s)	937
MseI	TTAA	6 cut(s)	54, 146, 741, 932, 1128, 1379
MslI	CAYNNNNRTG	2 cut(s)	252, 530
MspA1I	CMGCKG	4 cut(s)	73, 404, 899, 1282
MspI	CCGG	6 cut(s)	848, 925, 938, 1006, 1511, 1708
MspR9I	CCNGG	4 cut(s)	272, 842, 1511, 1724
MunI	CAATTG	3 cut(s)	105, 1065, 1737
MvaI	CCWGG	3 cut(s)	272, 842, 1724
MwoI	GCNNNNNNNGC	4 cut(s)	259, 1552, 1724, 1733
NciI	CCSGG	1 cut(s)	1511
NdeI	CATATG	2 cut(s)	229, 853
NdeII	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
NheI	GCTAGC	1 cut(s)	1213
NlaIV	GGNNCC	6 cut(s)	702, 819, 846, 1315, 1521, 1692
NsbI	TGCGCA	1 cut(s)	252
NspI	RCATGY	7 cut(s)	251, 257, 438, 529, 1004, 1327, 1536
PaeI	GCATGC	2 cut(s)	251, 1327
PaeR7I	CTCGAG	1 cut(s)	423
PagI	TCATGA	1 cut(s)	1749
PciI	ACATGT	2 cut(s)	1000, 1532
PciSI	GCTCTTC	2 cut(s)	120, 1734
PfeI	GAWTC	4 cut(s)	647, 989, 1156, 1170
PflFI	GACNNNGTC	1 cut(s)	1343
PflMI	CCANNNNNTGG	1 cut(s)	233
PleI	GAGTC	4 cut(s)	248, 331, 434, 651
PmaCI	CACGTG	1 cut(s)	113
PmlI	CACGTG	1 cut(s)	113
PpsI	GAGTC	4 cut(s)	248, 331, 434, 651
Ppu21I	YACGTR	1 cut(s)	113
PpuMI	RGGWCCY	2 cut(s)	1438, 1690
PscI	ACATGT	2 cut(s)	1000, 1532
PshBI	ATTAAT	1 cut(s)	146
PsiI	TTATAA	1 cut(s)	1028
Psp124BI	GAGCTC	1 cut(s)	1683
Psp5II	RGGWCCY	2 cut(s)	1438, 1690
Psp6I	CCWGG	3 cut(s)	270, 840, 1722
PspCI	CACGTG	1 cut(s)	113
PspFI	CCCAGC	1 cut(s)	1282
PspGI	CCWGG	3 cut(s)	270, 840, 1722
PspN4I	GGNNCC	6 cut(s)	702, 819, 846, 1315, 1521, 1692
PspPI	GGNCC	6 cut(s)	818, 838, 1403, 1438, 1520, 1690
PspPPI	RGGWCCY	2 cut(s)	1438, 1690
PspXI	VCTCGAGB	1 cut(s)	423
PstI	CTGCAG	1 cut(s)	1617
PstNI	CAGNNNCTG	2 cut(s)	578, 902
PsuI	RGATCY	1 cut(s)	625
PsyI	GACNNNGTC	1 cut(s)	1343
PvuII	CAGCTG	4 cut(s)	73, 404, 899, 1282
RsaI	GTAC	3 cut(s)	189, 1259, 1581
RsaNI	GTAC	3 cut(s)	188, 1258, 1580
RseI	CAYNNNNRTG	2 cut(s)	252, 530
SacI	GAGCTC	1 cut(s)	1683
SapI	GCTCTTC	2 cut(s)	120, 1734
SaqAI	TTAA	6 cut(s)	54, 146, 741, 932, 1128, 1379
Sau3AI	GATC	6 cut(s)	494, 625, 640, 1147, 1636, 1741
Sau96I	GGNCC	6 cut(s)	818, 838, 1403, 1438, 1520, 1690
SchI	GAGTC	4 cut(s)	249, 332, 435, 651
ScrFI	CCNGG	4 cut(s)	272, 842, 1511, 1724
SduI	GDGCHC	6 cut(s)	118, 469, 705, 1318, 1557, 1683
SexAI	ACCWGGT	1 cut(s)	840
SfaNI	GCATC	2 cut(s)	1048, 1351
SfcI	CTRYAG	1 cut(s)	1613
Sfr274I	CTCGAG	1 cut(s)	423
SinI	GGWCC	5 cut(s)	838, 1403, 1438, 1520, 1690
SlaI	CTCGAG	1 cut(s)	423
SmiMI	CAYNNNNRTG	2 cut(s)	252, 530
SmlI	CTYRAG	2 cut(s)	423, 633
SmoI	CTYRAG	2 cut(s)	423, 633
SphI	GCATGC	2 cut(s)	251, 1327
SsiI	CCGC	6 cut(s)	20, 76, 91, 203, 266, 588
SspMI	CTAG	4 cut(s)	462, 560, 1214, 1762
SstI	GAGCTC	1 cut(s)	1683
StyD4I	CCNGG	4 cut(s)	270, 840, 1509, 1722
StyI	CCWWGG	1 cut(s)	559
TaaI	ACNGT	3 cut(s)	289, 506, 580
TaiI	ACGT	1 cut(s)	115
TaqI	TCGA	7 cut(s)	243, 424, 655, 868, 1173, 1240, 1474
TaqII	GACCGA	1 cut(s)	1391
TatI	WGTACW	1 cut(s)	1257
TfiI	GAWTC	4 cut(s)	647, 989, 1156, 1170
Tru1I	TTAA	6 cut(s)	54, 146, 741, 932, 1128, 1379
Tru9I	TTAA	6 cut(s)	54, 146, 741, 932, 1128, 1379
TscAI	CASTG	4 cut(s)	292, 474, 1334, 1557
TspDTI	ATGAA	4 cut(s)	30, 211, 437, 1122
TspGWI	ACGGA	1 cut(s)	851
TspRI	CASTG	4 cut(s)	292, 474, 1334, 1557
Tth111I	GACNNNGTC	1 cut(s)	1343
Van91I	CCANNNNNTGG	1 cut(s)	233
VneI	GTGCAC	1 cut(s)	465
VpaK11BI	GGWCC	5 cut(s)	838, 1403, 1438, 1520, 1690
VspI	ATTAAT	1 cut(s)	146
XagI	CCTNNNNNAGG	1 cut(s)	304
XapI	RAATTY	4 cut(s)	566, 917, 1109, 1592
XceI	RCATGY	7 cut(s)	251, 257, 438, 529, 1004, 1327, 1536
XhoI	CTCGAG	1 cut(s)	423
XmaJI	CCTAGG	1 cut(s)	559
XmiI	GTMKAC	1 cut(s)	1347
XspI	CTAG	4 cut(s)	462, 560, 1214, 1762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.