Rorug06G0466900

Serine threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002203
Physical Location & Seq
Reverse (-)
59121634 .. 59126743
5110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0466900.1

Sequence Viewer

Length: 861 bp
ATGGCACAGATTTCCCTTTCCTCTTCCTCTTCTTGTCCTCTAATTCACCGGCGAATCTTTCTTCCTTGTGCTTTTTCAATCCAAGCTACCTCGACGACTTCAAATTTGCAGCCGCCGAAAGTCGTCGTCACCAGAGAGCGCGGCAAGAATGCCAAACTCATCACCTCTCTGGCGAAGCAGGGAATCAGCTGTTTGGAGCTTCCCCTCATCCAGCATACACGCGAGCCTGATCGAGATAGGCTTTCTACTGTATTGACTGATACTGCGTTTGACTGGATTGTCATAACTTCCCCTGAAGCTGGTTCAGTCTTTTTGGAGTCATGGAAGGCTGCCGGAACTCCAACTGTTAAGGTTGGTGTCGTCGGGGCTGGTACAGCAAGTGTCTTTGAGCAAGTACTGCAGTCATCAAAGAACTCCCTCAGTCTTGCCTTTGTGCCATCAAAAGGCAAGGTTTTGGCTTCAGAGCTCCCTAAGAATAGGAAAGACAAATGCACAGTCCTATATCCTGCTTCTGCAAAAGCTGGCAATGAGATTGAGGAAGGCCTGTCCAATCGTGGATTTGAGGTCACAAGGCTAAATACGTACACCACGGGACCTGTTGACCACGTCGATCAAATGGTTTTGAAGCAGGCTCTTTCTGCCCCTGTTGTTGCAGTTGCTTCTCCTTCTGCAGTTCATGCTTGGGTCAATCTTATTTCTGAGTCTGAGCAATGGAACAATTCGGTTGCCTGTATTGGTGAGACAACTGCCAAGGCTGCTAGGAGATTAGGCTTGAGAAATGTTTACTATCCAGTGCAACCAGGTCTTGAAGGATGGGTGGATAGCATTGTTGAAGCATTGGGAGCAAATGTCCATTTGTAG

Protein Analysis

286

Amino Acids

30.66

Weight (kDa)

8.79

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 53 - 273 2.8e-43 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AccII CGCG 2 cut(s) 141, 222
AciI CCGC 2 cut(s) 113, 141
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 2 cut(s) 315, 444
AfaI GTAC 3 cut(s) 373, 396, 584
AfiI CCNNNNNNNGG 2 cut(s) 299, 443
AgsI TTSAA 5 cut(s) 78, 102, 625, 809, 833
AjiI CACGTC 1 cut(s) 607
AjnI CCWGG 1 cut(s) 799
AluBI AGCT 6 cut(s) 86, 189, 199, 299, 466, 521
AluI AGCT 6 cut(s) 86, 189, 199, 299, 466, 521
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 734
AoxI GGCC 1 cut(s) 541
ApeKI GCWGC 3 cut(s) 109, 329, 755
ApoI RAATTY 1 cut(s) 103
ArsI GACNNNNNNTTYG 4 cut(s) 88, 120, 480, 512
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 4 cut(s) 38, 121, 154, 749
AvaII GGWCC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 121, 316, 742
BccI CCATC 2 cut(s) 445, 807
BciT130I CCWGG 1 cut(s) 801
BcoDI GTCTC 1 cut(s) 734
BfaI CTAG 1 cut(s) 759
BfmI CTRYAG 2 cut(s) 398, 669
BisI GCNGC 5 cut(s) 110, 113, 142, 330, 756
BlsI GCNGC 5 cut(s) 111, 114, 143, 331, 757
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 801
Bme18I GGWCC 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 793
BsaAI YACGTR 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 588, 750
BsaXI ACNNNNNCTCC 1 cut(s) 834
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 443
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 278, 791
Bse3DI GCAATG 2 cut(s) 532, 716
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 2 cut(s) 588, 750
BseGI GGATG 2 cut(s) 207, 818
BseLI CCNNNNNNNGG 2 cut(s) 299, 443
BseMI GCAATG 2 cut(s) 532, 716
BseMII CTCAG 3 cut(s) 433, 690, 696
BseNI ACTGG 2 cut(s) 278, 791
BseXI GCAGC 3 cut(s) 121, 316, 742
Bsh1236I CGCG 2 cut(s) 141, 222
BshFI GGCC 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 2 cut(s) 49, 333
BslFI GGGAC 1 cut(s) 606
BslI CCNNNNNNNGG 2 cut(s) 299, 443
BsmAI GTCTC 1 cut(s) 734
BsmFI GGGAC 1 cut(s) 606
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 2 cut(s) 229, 610
BspACI CCGC 2 cut(s) 113, 141
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 432, 691, 697
BspFNI CGCG 2 cut(s) 141, 222
BspLI GGNNCC 1 cut(s) 594
BspMAI CTGCAG 2 cut(s) 402, 673
BsrDI GCAATG 2 cut(s) 532, 716
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 2 cut(s) 278, 791
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 588, 750
BssMI GATC 2 cut(s) 229, 610
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 3 cut(s) 250, 346, 496
Bst6I CTCTTC 2 cut(s) 28, 34
BstAPI GCANNNNNTGC 2 cut(s) 397, 677
BstBAI YACGTR 1 cut(s) 582
BstC8I GCNNGC 3 cut(s) 224, 523, 630
BstDEI CTNAG 4 cut(s) 419, 471, 699, 705
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 207, 818
BstFNI CGCG 2 cut(s) 141, 222
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 2 cut(s) 232, 613
BstMAI GTCTC 1 cut(s) 734
BstMBI GATC 2 cut(s) 229, 610
BstMWI GCNNNNNNNGC 6 cut(s) 374, 397, 638, 677, 755, 842
BstNI CCWGG 1 cut(s) 801
BstSCI CCNGG 1 cut(s) 799
BstSFI CTRYAG 2 cut(s) 398, 669
BstSNI TACGTA 1 cut(s) 582
BstUI CGCG 2 cut(s) 141, 222
BstV1I GCAGC 3 cut(s) 121, 316, 742
BsuRI GGCC 1 cut(s) 543
BtgI CCRYGG 1 cut(s) 588
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 2 cut(s) 207, 818
BtsIMutI CAGTG 1 cut(s) 798
Cac8I GCNNGC 3 cut(s) 224, 523, 630
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 593
CsiI ACCWGGT 1 cut(s) 799
Csp6I GTAC 3 cut(s) 372, 395, 583
CviAII CATG 2 cut(s) 321, 677
CviQI GTAC 3 cut(s) 372, 395, 583
DdeI CTNAG 4 cut(s) 419, 471, 699, 705
DpnI GATC 2 cut(s) 231, 612
DpnII GATC 2 cut(s) 229, 610
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 28, 34
EarI CTCTTC 2 cut(s) 28, 34
Ecl136II GAGCTC 1 cut(s) 466
Eco105I TACGTA 1 cut(s) 582
Eco130I CCWWGG 1 cut(s) 750
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 315, 444
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 2 cut(s) 324, 680
FaiI YATR 5 cut(s) 216, 284, 322, 502, 678
FaqI GGGAC 1 cut(s) 606
FatI CATG 2 cut(s) 320, 676
Fnu4HI GCNGC 5 cut(s) 110, 113, 142, 330, 756
FokI GGATG 2 cut(s) 194, 825
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 5 cut(s) 110, 113, 142, 330, 756
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 140
GluI GCNGC 5 cut(s) 110, 113, 142, 330, 756
HaeIII GGCC 1 cut(s) 543
HapII CCGG 2 cut(s) 49, 333
HhaI GCGC 1 cut(s) 141
Hin1II CATG 2 cut(s) 324, 680
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 4 cut(s) 54, 183, 317, 701
HpaII CCGG 2 cut(s) 49, 333
HphI GGTGA 4 cut(s) 38, 121, 154, 749
Hpy166II GTNNAC 3 cut(s) 585, 601, 784
Hpy188I TCNGA 3 cut(s) 463, 700, 706
Hpy188III TCNNGA 2 cut(s) 233, 806
Hpy8I GTNNAC 3 cut(s) 585, 601, 784
Hpy99I CGWCG 4 cut(s) 97, 128, 365, 611
HpyAV CCTTC 4 cut(s) 319, 533, 675, 803
HpyCH4III ACNGT 3 cut(s) 250, 346, 496
HpyCH4IV ACGT 2 cut(s) 581, 606
HpyCH4V TGCA 7 cut(s) 109, 400, 492, 515, 653, 671, 796
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 397, 638, 677, 755, 842
HpyF3I CTNAG 4 cut(s) 419, 471, 699, 705
HpySE526I ACGT 2 cut(s) 581, 606
Hsp92II CATG 2 cut(s) 324, 680
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 229, 610
LmnI GCTCC 3 cut(s) 196, 471, 842
Lsp1109I GCAGC 3 cut(s) 121, 316, 742
MabI ACCWGGT 1 cut(s) 799
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 581, 606
MaeIII GTNAC 2 cut(s) 127, 565
MalI GATC 2 cut(s) 231, 612
MboI GATC 2 cut(s) 229, 610
MboII GAAGA 3 cut(s) 15, 21, 53
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 3 cut(s) 42, 103, 718
MlyI GAGTC 2 cut(s) 326, 710
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 9 cut(s) 31, 37, 48, 100, 175, 215, 428, 529, 556
MseI TTAA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 2 cut(s) 49, 333
MspR9I CCNGG 1 cut(s) 801
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 1 cut(s) 801
MvnI CGCG 2 cut(s) 141, 222
MwoI GCNNNNNNNGC 6 cut(s) 374, 397, 638, 677, 755, 842
NdeII GATC 2 cut(s) 229, 610
NlaIII CATG 2 cut(s) 324, 680
NlaIV GGNNCC 1 cut(s) 594
NmuCI GTSAC 2 cut(s) 127, 565
PceI AGGCCT 1 cut(s) 543
PctI GAATGC 1 cut(s) 154
PfeI GAWTC 2 cut(s) 54, 183
PflFI GACNNNGTC 1 cut(s) 605
PkrI GCNGC 5 cut(s) 111, 114, 143, 331, 757
PleI GAGTC 2 cut(s) 325, 709
PpsI GAGTC 2 cut(s) 325, 709
Ppu21I YACGTR 1 cut(s) 582
PpuMI RGGWCCY 1 cut(s) 593
Psp124BI GAGCTC 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 1 cut(s) 593
PspPPI RGGWCCY 1 cut(s) 593
PstI CTGCAG 2 cut(s) 402, 673
PsyI GACNNNGTC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 189
RsaI GTAC 3 cut(s) 373, 396, 584
RsaNI GTAC 3 cut(s) 372, 395, 583
SacI GAGCTC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 5 cut(s) 110, 113, 142, 330, 756
Sau3AI GATC 2 cut(s) 229, 610
Sau96I GGNCC 1 cut(s) 593
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 2 cut(s) 326, 710
ScrFI CCNGG 1 cut(s) 801
SduI GDGCHC 1 cut(s) 468
SexAI ACCWGGT 1 cut(s) 799
SfcI CTRYAG 2 cut(s) 398, 669
SgrAI CRCCGGYG 1 cut(s) 48
SinI GGWCC 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
SnaBI TACGTA 1 cut(s) 582
Sse9I AATT 3 cut(s) 42, 103, 718
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 2 cut(s) 113, 141
SspMI CTAG 1 cut(s) 759
SstI GAGCTC 1 cut(s) 468
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 799
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 3 cut(s) 250, 346, 496
TaiI ACGT 2 cut(s) 584, 609
TaqI TCGA 3 cut(s) 92, 232, 609
TasI AATT 3 cut(s) 42, 103, 718
TatI WGTACW 1 cut(s) 394
TauI GCSGC 2 cut(s) 115, 144
TfiI GAWTC 2 cut(s) 54, 183
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 798
TseFI GTSAC 2 cut(s) 127, 565
TseI GCWGC 3 cut(s) 109, 329, 755
Tsp45I GTSAC 2 cut(s) 127, 565
TspDTI ATGAA 1 cut(s) 665
TspRI CASTG 1 cut(s) 798
Tth111I GACNNNGTC 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 1 cut(s) 103
XspI CTAG 1 cut(s) 759
ZrmI AGTACT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.