Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002203

Physical Location & Seq

Genomic Coordinates Reverse (-)

30644251 .. 30649637

Gene Structure 5387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1225600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGCTATGGTTGATGAACGTTTATACCCGATTGCGGTTTTAATTGACGAGCTTAAGAATGAAGACATTCAGCTCCGGTTGAACTCAATCCGGAGGCTTTCCACGATTGCCCGTGCGCTGGGAGAAGAGAGGACCAGGAAGGAACTGATTCCTTTCCTCAGTGAGAACAACGATGATGATGATGAAGTGCTTCTGGCAATGGCGGAAGAGTTGGGGGTGTTCATCCCCTATGTAGGTGGTGTGGAGCATGCCAACATTCTGCTTCCGCCTTTGGAAACACTCTGCACTGTTGAGGAAACATGTGTGAGGGATAAGGCGGTGGATTCGTTGTGTAAAATTGGGGGGCAGATGAGAGAAAAGGACTTGGTTGAATACTTCATTCCTTTGGTTAAGAGACTAGCTGCTGGCGAGTGGTTTACAGCCCGAGTTTCATCCTGTGGTTTGTTTCATATTGCTTATACTAGTGCACCAGAAACTTTAAAAACTGAACTACGAACGACATACAGTCAACTGTGCCAAGATGACATGCCCATGGTTAGGAGATCTGCTGCAAGTAACCTGGGAAAATTTGCTGGGACTGTAGAAGCTGTGCACCTCAAGGCTGACATAATGTCTATATTTGAGGATTTGACGCAGGATGATCAGGATTCTGTTCGGTTATTGGCCGTGGAGGGTTTTGCAGCTCTTGGGAAGTTGTTGGAGCCTCAAGATTGTGTGGCCCATATTCTTCCTGTCATTGTTAACTTTTCACAGGATAAGTCTTGGCGTGTTCGATACATGGTTGCAAATCAATTATATGAGCTTTGTGAAGCTGTTGGTCCAGAAGCTACAAGGTCGGACCTGGTGCCTGCATACGTTCGGCTTCTTCGGGATAATGAGGCTGAAGTACGAATAGCTGCTGCAGGAAAAGTAACTAAGTTTTGCCGAATTGTGAATCCAGAACTTGCCATTCAGCAGATCCTTCCTTTCGTAAAGGAATTGTCGACAGATTCTTCCCAGCATGTTCGTTCTGCATTGGCTTCAGTAATAATGGGAATGGCACAAGTTTTAGGAAAGGATGCAACTATAGAGCAGCTGCTGCCTATTTTTCTTTCTCTTCTAAAAGACGAGTTTCCTGATGTGCGATTGAATATTATTAGCAAGCTTGATCAAGTCAACGAGGTAATTGGGATTGATATGCTGTCCCAGTCTTTGTTGCCGGCAATTGTTGAACTTGCAGAGGATAGACATTGGAGGGTTCGGCTTGCGATAATAGAATACATTCCTTTATTGGCAAGTCAGTTGGGTGTTGAGTTTTTTGATGATAAGCTTGGCTCTCTCTGCATGCAGTGGTTAAATGATAAGGTTTACTCAATACGTGATGCAGCTGCTAATAATGTGAAGCGCCTTGCAGAAGAATTCGGCCCCGAATGGGCAATGCAGCATATAGTTCCACAGGTTTTGGAAATGATAAGCAACCCCCACTATTTGTATCGGATGACCATTCTACATGCCATTTCCCTACTTGCCCCTGTTTTGGGATCAGAACTCACTTGTTCCAGGCTTGTACCGGTGATAGTCACTGCATCAAAAGACAGGGTACCAAACATCAAGTTCAATGTGGCTAAGGTGTTGCAGTCGATTATTCCGATAGTTGATCAGTCGGTGGTGGAGAAAACTATTTGTCCTTGTTTGCTTGAGCTTAGTGAGGATCCCGACGTTGATGTAAGGTCCTTTGCTAGCCAAGCCCTTCAGTCAAGCGAACAGGTCATGATGTCTCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04111 ko04113 ko04114 ko04151 ko04152 ko04261 ko04350 ko04390 ko04391 ko04530 ko04728 ko04730 ko05142 ko05160 ko05165 map03015 map04071 map04111 map04113 map04114 map04151 map04152 map04261 map04350 map04390 map04391 map04530 map04728 map04730 map05142 map05160 map05165

Pfam Domains

Protein Families

HEAT HEAT_2

Protein Analysis

588

Amino Acids

65.66

Weight (kDa)

4.9

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VPS15-like_hel	PF22956	198 - 309	4.8e-08	VPS15-like, helical domain
PPP2R1A-like_HEAT	PF22646	274 - 351	1.8e-28	PPP2R1A-like HEAT repeat
TPR_DNAAF5	PF25757	274 - 458	2e-07	Dynein axonemal assembly factor 5-like, TPR repeats
VPS15-like_hel	PF22956	358 - 516	3.1e-07	VPS15-like, helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13320 AT1G13320 AT1G13320 AT1G25490 AT3G25800 AT3G25800 AT3G25800
fragaria_vesca	FvH4_4g27700 FvH4_4g27700 FvH4_5g12331 FvH4_5g12331
malus_domestica	MD06G1214700.v1.1 MD13G1044500.v1.1 MD14G1225600.v1.1 MD16G1045400.v1.1
prunus_persica	Prupe.1G307900_v2.0.a1 Prupe.1G307900_v2.0.a1 Prupe.5G220100_v2.0.a1
pyrus_communis	pycom06g19130 pycom13g03910 pycom14g18680 pycom16g03990
rosa_chinensis	RchiOBHm_Chr4g0435561 RchiOBHm_Chr7g0185211
rosa_laevigata	RLG00000006582
rosa_multiflora	Rmu_sc0001940.1_g000038
rosa_roxburghii	Rroxscaffold_3G00269150 Rroxscaffold_5G00376790
rosa_rugosa	Rorug04G0286500 Rorug04G0286500 Rorug04G0286600 Rorug06G0466800 Rorug06G0466900
rosa_samantha	Rh4AG342000 Rh4BG350200 Rh4CG364900 Rh4DG344500 Rh7AG076000 Rh7BG071300 Rh7CG072600 Rh7DG072000
rosa_wichuraiana	Rw4G029830 Rw7G005990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1579
AccB1I	GGYRCC	2 cut(s)	844, 1579
AccI	GTMKAC	1 cut(s)	983
AccIII	TCCGGA	1 cut(s)	90
AciI	CCGC	4 cut(s)	35, 203, 266, 317
AclI	AACGTT	1 cut(s)	19
AclWI	GGATC	4 cut(s)	952, 1528, 1685, 1698
AcoI	YGGCCR	1 cut(s)	663
AcsI	RAATTY	2 cut(s)	566, 1397
AcuI	CTGAAG	3 cut(s)	903, 1005, 1715
AfaI	GTAC	3 cut(s)	888, 1548, 1581
AfiI	CCNNNNNNNGG	7 cut(s)	34, 118, 233, 537, 1411, 1516, 1517
AflII	CTTAAG	1 cut(s)	53
AflIII	ACRYGT	1 cut(s)	299
AgeI	ACCGGT	1 cut(s)	1549
AgsI	TTSAA	5 cut(s)	82, 371, 1129, 1211, 1597
AhdI	GACNNNNNGTC	2 cut(s)	504, 610
AhlI	ACTAGT	1 cut(s)	461
AjnI	CCWGG	4 cut(s)	134, 558, 840, 1538
Alw21I	GWGCWC	2 cut(s)	469, 594
Alw26I	GTCTC	1 cut(s)	388
Alw44I	GTGCAC	2 cut(s)	465, 590
AlwI	GGATC	4 cut(s)	952, 1528, 1685, 1698
AlwNI	CAGNNNCTG	1 cut(s)	1078
Ama87I	CYCGRG	1 cut(s)	423
Aor13HI	TCCGGA	1 cut(s)	90
AoxI	GGCC	3 cut(s)	663, 717, 1402
ApaLI	GTGCAC	2 cut(s)	465, 590
ApoI	RAATTY	2 cut(s)	566, 1397
AsiGI	ACCGGT	1 cut(s)	1549
Asp700I	GAANNNNTTC	4 cut(s)	66, 147, 189, 1260
Asp718I	GGTACC	1 cut(s)	1579
AspLEI	GCGC	2 cut(s)	118, 1386
AspS9I	GGNCC	6 cut(s)	132, 718, 818, 838, 1403, 1710
AsuHPI	GGTGA	1 cut(s)	1564
AsuNHI	GCTAGC	1 cut(s)	1718
AvaI	CYCGRG	1 cut(s)	423
AvaII	GGWCC	4 cut(s)	132, 818, 838, 1710
BaeGI	GKGCMC	2 cut(s)	469, 594
BamHI	GGATCC	1 cut(s)	1690
BanI	GGYRCC	2 cut(s)	844, 1579
BbsI	GAAGAC	1 cut(s)	69
Bbv12I	GWGCWC	2 cut(s)	469, 594
BceAI	ACGGC	1 cut(s)	650
BciT130I	CCWGG	4 cut(s)	136, 560, 842, 1540
BclI	TGATCA	3 cut(s)	640, 1147, 1636
BcoDI	GTCTC	1 cut(s)	388
BcuI	ACTAGT	1 cut(s)	461
BfaI	CTAG	4 cut(s)	398, 462, 1719, 1762
BfmI	CTRYAG	3 cut(s)	579, 900, 1065
BfoI	RGCGCY	1 cut(s)	1387
BfrI	CTTAAG	1 cut(s)	53
BglII	AGATCT	1 cut(s)	542
Bme1390I	CCNGG	4 cut(s)	136, 560, 842, 1540
Bme18I	GGWCC	4 cut(s)	132, 818, 838, 1710
BmeRI	GACNNNNNGTC	2 cut(s)	504, 610
BmeT110I	CYCGRG	1 cut(s)	423
BmgT120I	GGNCC	6 cut(s)	132, 718, 818, 838, 1403, 1710
BmiI	GGNNCC	5 cut(s)	702, 846, 1405, 1581, 1692
BmrFI	CCNGG	4 cut(s)	136, 560, 842, 1540
BmrI	ACTGGG	1 cut(s)	1180
BmsI	GCATC	3 cut(s)	1048, 1351, 1574
BmtI	GCTAGC	1 cut(s)	1722
BmuI	ACTGGG	1 cut(s)	1180
BpiI	GAAGAC	1 cut(s)	69
Bpu10I	CCTNAGC	1 cut(s)	1605
BpuEI	CTTGAG	3 cut(s)	581, 690, 1697
BsaAI	YACGTR	1 cut(s)	1358
BsaJI	CCNNGG	3 cut(s)	531, 559, 666
BsaWI	WCCGGW	3 cut(s)	75, 90, 1549
Bsc4I	CCNNNNNNNGG	7 cut(s)	34, 118, 233, 537, 1411, 1516, 1517
Bse118I	RCCGGY	2 cut(s)	1198, 1549
Bse1I	ACTGG	1 cut(s)	1186
Bse3DI	GCAATG	2 cut(s)	204, 1422
BseAI	TCCGGA	1 cut(s)	90
BseBI	CCWGG	4 cut(s)	136, 560, 842, 1540
BseDI	CCNNGG	3 cut(s)	531, 559, 666
BseGI	GGATG	5 cut(s)	222, 431, 643, 1063, 1482
BseLI	CCNNNNNNNGG	7 cut(s)	34, 118, 233, 537, 1411, 1516, 1517
BseMI	GCAATG	2 cut(s)	204, 1422
BseMII	CTCAG	1 cut(s)	172
BseNI	ACTGG	1 cut(s)	1186
BseSI	GKGCMC	2 cut(s)	469, 594
BseYI	CCCAGC	3 cut(s)	118, 572, 996
BsgI	GTGCAG	1 cut(s)	268
BshFI	GGCC	3 cut(s)	665, 719, 1404
BshNI	GGYRCC	2 cut(s)	844, 1579
BshTI	ACCGGT	1 cut(s)	1549
BsiHKAI	GWGCWC	2 cut(s)	469, 594
BsiHKCI	CYCGRG	1 cut(s)	423
BsiSI	CCGG	4 cut(s)	76, 91, 1199, 1550
BslFI	GGGAC	2 cut(s)	589, 1168
BslI	CCNNNNNNNGG	7 cut(s)	34, 118, 233, 537, 1411, 1516, 1517
BsmAI	GTCTC	1 cut(s)	388
BsmFI	GGGAC	2 cut(s)	589, 1168
BsnI	GGCC	3 cut(s)	665, 719, 1404
BsoBI	CYCGRG	1 cut(s)	423
Bsp1286I	GDGCHC	2 cut(s)	469, 594
Bsp13I	TCCGGA	1 cut(s)	90
Bsp143I	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
Bsp19I	CCATGG	1 cut(s)	531
BspACI	CCGC	4 cut(s)	35, 203, 266, 317
BspANI	GGCC	3 cut(s)	665, 719, 1404
BspCNI	CTCAG	1 cut(s)	171
BspEI	TCCGGA	1 cut(s)	90
BspHI	TCATGA	1 cut(s)	1749
BspLI	GGNNCC	5 cut(s)	702, 846, 1405, 1581, 1692
BspMAI	CTGCAG	1 cut(s)	904
BspOI	GCTAGC	1 cut(s)	1722
BspPI	GGATC	4 cut(s)	952, 1528, 1685, 1698
BspT107I	GGYRCC	2 cut(s)	844, 1579
BspTI	CTTAAG	1 cut(s)	53
BsrDI	GCAATG	2 cut(s)	204, 1422
BsrFI	RCCGGY	2 cut(s)	1198, 1549
BsrI	ACTGG	1 cut(s)	1186
BssAI	RCCGGY	2 cut(s)	1198, 1549
BssECI	CCNNGG	3 cut(s)	531, 559, 666
BssMI	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
BssT1I	CCWWGG	1 cut(s)	531
Bst2UI	CCWGG	4 cut(s)	136, 560, 842, 1540
Bst4CI	ACNGT	4 cut(s)	289, 506, 513, 580
Bst6I	CTCTTC	3 cut(s)	120, 201, 1101
BstAFI	CTTAAG	1 cut(s)	53
BstAPI	GCANNNNNTGC	1 cut(s)	1078
BstBAI	YACGTR	1 cut(s)	1358
BstC8I	GCNNGC	8 cut(s)	249, 406, 849, 1142, 1200, 1245, 1325, 1720
BstDEI	CTNAG	4 cut(s)	158, 915, 1605, 1682
BstDSI	CCRYGG	2 cut(s)	531, 666
BstF5I	GGATG	5 cut(s)	222, 431, 643, 1063, 1482
BstH2I	RGCGCY	1 cut(s)	1387
BstHHI	GCGC	2 cut(s)	118, 1386
BstKTI	GATC	7 cut(s)	545, 643, 960, 1150, 1523, 1639, 1693
BstMAI	GTCTC	1 cut(s)	388
BstMBI	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
BstMWI	GCNNNNNNNGC	3 cut(s)	1078, 1320, 1724
BstNI	CCWGG	4 cut(s)	136, 560, 842, 1540
BstNSI	RCATGY	6 cut(s)	251, 303, 529, 1004, 1327, 1493
BstSCI	CCNGG	4 cut(s)	134, 558, 840, 1538
BstSFI	CTRYAG	3 cut(s)	579, 900, 1065
BstSLI	GKGCMC	2 cut(s)	469, 594
BstV2I	GAAGAC	1 cut(s)	69
BstX2I	RGATCY	3 cut(s)	542, 957, 1690
BstYI	RGATCY	3 cut(s)	542, 957, 1690
BsuRI	GGCC	3 cut(s)	665, 719, 1404
BtgI	CCRYGG	2 cut(s)	531, 666
BtsCI	GGATG	5 cut(s)	222, 431, 643, 1063, 1482
BtsI	GCAGTG	2 cut(s)	1334, 1560
BtsIMutI	CAGTG	4 cut(s)	166, 285, 1334, 1560
Cac8I	GCNNGC	8 cut(s)	249, 406, 849, 1142, 1200, 1245, 1325, 1720
CaiI	CAGNNNCTG	1 cut(s)	1078
CciI	TCATGA	1 cut(s)	1749
CfoI	GCGC	2 cut(s)	118, 1386
Cfr10I	RCCGGY	2 cut(s)	1198, 1549
Cfr13I	GGNCC	6 cut(s)	132, 718, 818, 838, 1403, 1710
CseI	GACGC	1 cut(s)	640
CsiI	ACCWGGT	1 cut(s)	840
Csp6I	GTAC	3 cut(s)	887, 1547, 1580
CspAI	ACCGGT	1 cut(s)	1549
CspCI	CAANNNNNGTGG	2 cut(s)	1422, 1457
CviAII	CATG	9 cut(s)	248, 300, 526, 532, 778, 1001, 1324, 1490, 1750
CviQI	GTAC	3 cut(s)	887, 1547, 1580
DdeI	CTNAG	4 cut(s)	158, 915, 1605, 1682
DpnI	GATC	7 cut(s)	544, 642, 959, 1149, 1522, 1638, 1692
DpnII	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
DraI	TTTAAA	1 cut(s)	480
DriI	GACNNNNNGTC	2 cut(s)	504, 610
EaeI	YGGCCR	1 cut(s)	663
Eam1104I	CTCTTC	3 cut(s)	120, 201, 1101
Eam1105I	GACNNNNNGTC	2 cut(s)	504, 610
EarI	CTCTTC	3 cut(s)	120, 201, 1101
EciI	GGCGGA	2 cut(s)	218, 255
Eco130I	CCWWGG	1 cut(s)	531
Eco47I	GGWCC	4 cut(s)	132, 818, 838, 1710
Eco57I	CTGAAG	3 cut(s)	903, 1005, 1715
Eco88I	CYCGRG	1 cut(s)	423
EcoO109I	RGGNCCY	1 cut(s)	1710
EcoRI	GAATTC	1 cut(s)	1397
EcoRII	CCWGG	4 cut(s)	134, 558, 840, 1538
EcoT14I	CCWWGG	1 cut(s)	531
ErhI	CCWWGG	1 cut(s)	531
FaeI	CATG	9 cut(s)	251, 303, 529, 535, 781, 1004, 1327, 1493, 1753
FaqI	GGGAC	2 cut(s)	589, 1168
FatI	CATG	9 cut(s)	247, 299, 525, 531, 777, 1000, 1323, 1489, 1749
FbaI	TGATCA	3 cut(s)	640, 1147, 1636
FblI	GTMKAC	1 cut(s)	983
FokI	GGATG	5 cut(s)	209, 418, 650, 1070, 1489
FspBI	CTAG	4 cut(s)	398, 462, 1719, 1762
GlaI	GCGC	2 cut(s)	117, 1385
GsaI	CCCAGC	3 cut(s)	122, 576, 1000
HaeII	RGCGCY	1 cut(s)	1387
HaeIII	GGCC	3 cut(s)	665, 719, 1404
HapII	CCGG	4 cut(s)	76, 91, 1199, 1550
HgaI	GACGC	1 cut(s)	640
HhaI	GCGC	2 cut(s)	118, 1386
Hin1II	CATG	9 cut(s)	251, 303, 529, 535, 781, 1004, 1327, 1493, 1753
Hin6I	GCGC	2 cut(s)	116, 1384
HinP1I	GCGC	2 cut(s)	116, 1384
HincII	GTYRAC	4 cut(s)	509, 742, 984, 1156
HindII	GTYRAC	4 cut(s)	509, 742, 984, 1156
HindIII	AAGCTT	2 cut(s)	1142, 1307
HinfI	GANTC	5 cut(s)	148, 323, 647, 934, 989
HpaI	GTTAAC	1 cut(s)	742
HpaII	CCGG	4 cut(s)	76, 91, 1199, 1550
HphI	GGTGA	1 cut(s)	1564
Hpy166II	GTNNAC	8 cut(s)	417, 467, 509, 592, 742, 984, 1156, 1348
Hpy188I	TCNGA	4 cut(s)	838, 1476, 1525, 1629
Hpy188III	TCNNGA	9 cut(s)	91, 644, 707, 821, 869, 938, 1115, 1694, 1750
Hpy8I	GTNNAC	8 cut(s)	417, 467, 509, 592, 742, 984, 1156, 1348
Hpy99I	CGWCG	1 cut(s)	1700
HpyAV	CCTTC	3 cut(s)	133, 971, 1739
HpyCH4III	ACNGT	4 cut(s)	289, 506, 513, 580
HpyCH4IV	ACGT	4 cut(s)	19, 855, 1357, 1698
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1078, 1320, 1724
HpyF3I	CTNAG	4 cut(s)	158, 915, 1605, 1682
HpySE526I	ACGT	4 cut(s)	19, 855, 1357, 1698
Hsp92II	CATG	9 cut(s)	251, 303, 529, 535, 781, 1004, 1327, 1493, 1753
HspAI	GCGC	2 cut(s)	116, 1384
Kpn2I	TCCGGA	1 cut(s)	90
KpnI	GGTACC	1 cut(s)	1583
KroI	GCCGGC	1 cut(s)	1198
KroNI	GCCGGC	1 cut(s)	1200
Ksp22I	TGATCA	3 cut(s)	640, 1147, 1636
KspAI	GTTAAC	1 cut(s)	742
Kzo9I	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
LmnI	GCTCC	3 cut(s)	78, 244, 700
LweI	GCATC	3 cut(s)	1048, 1351, 1574
MabI	ACCWGGT	1 cut(s)	840
MaeI	CTAG	4 cut(s)	398, 462, 1719, 1762
MaeII	ACGT	4 cut(s)	19, 855, 1357, 1698
MaeIII	GTNAC	3 cut(s)	554, 910, 1558
MalI	GATC	7 cut(s)	544, 642, 959, 1149, 1522, 1638, 1692
MboI	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
MboII	GAAGA	8 cut(s)	74, 137, 218, 719, 857, 984, 1088, 1406
MfeI	CAATTG	1 cut(s)	1203
MflI	RGATCY	3 cut(s)	542, 957, 1690
MhlI	GDGCHC	2 cut(s)	469, 594
MluCI	AATT	9 cut(s)	42, 336, 566, 791, 927, 977, 1164, 1203, 1397
MmeI	TCCRAC	2 cut(s)	678, 816
MroI	TCCGGA	1 cut(s)	90
MroNI	GCCGGC	1 cut(s)	1198
MroXI	GAANNNNTTC	4 cut(s)	66, 147, 189, 1260
MseI	TTAA	6 cut(s)	41, 54, 390, 479, 741, 1334
MslI	CAYNNNNRTG	1 cut(s)	530
MspA1I	CMGCKG	2 cut(s)	1075, 1367
MspCI	CTTAAG	1 cut(s)	53
MspI	CCGG	4 cut(s)	76, 91, 1199, 1550
MspR9I	CCNGG	4 cut(s)	136, 560, 842, 1540
MunI	CAATTG	1 cut(s)	1203
MvaI	CCWGG	4 cut(s)	136, 560, 842, 1540
MwoI	GCNNNNNNNGC	3 cut(s)	1078, 1320, 1724
NaeI	GCCGGC	1 cut(s)	1200
NcoI	CCATGG	1 cut(s)	531
NdeII	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
NgoMIV	GCCGGC	1 cut(s)	1198
NheI	GCTAGC	1 cut(s)	1718
NlaIII	CATG	9 cut(s)	251, 303, 529, 535, 781, 1004, 1327, 1493, 1753
NlaIV	GGNNCC	5 cut(s)	702, 846, 1405, 1581, 1692
NmuCI	GTSAC	1 cut(s)	1558
NspI	RCATGY	6 cut(s)	251, 303, 529, 1004, 1327, 1493
PaeI	GCATGC	2 cut(s)	251, 1327
PagI	TCATGA	1 cut(s)	1749
PciI	ACATGT	1 cut(s)	299
PcsI	WCGNNNNNNNCGW	1 cut(s)	1625
PdiI	GCCGGC	1 cut(s)	1200
PdmI	GAANNNNTTC	4 cut(s)	66, 147, 189, 1260
PfeI	GAWTC	5 cut(s)	148, 323, 647, 934, 989
PinAI	ACCGGT	1 cut(s)	1549
Ppu21I	YACGTR	1 cut(s)	1358
PpuMI	RGGWCCY	1 cut(s)	1710
PscI	ACATGT	1 cut(s)	299
Psp1406I	AACGTT	1 cut(s)	19
Psp5II	RGGWCCY	1 cut(s)	1710
Psp6I	CCWGG	4 cut(s)	134, 558, 840, 1538
PspFI	CCCAGC	3 cut(s)	118, 572, 996
PspGI	CCWGG	4 cut(s)	134, 558, 840, 1538
PspN4I	GGNNCC	5 cut(s)	702, 846, 1405, 1581, 1692
PspPI	GGNCC	6 cut(s)	132, 718, 818, 838, 1403, 1710
PspPPI	RGGWCCY	1 cut(s)	1710
PstI	CTGCAG	1 cut(s)	904
PstNI	CAGNNNCTG	1 cut(s)	1078
PsuI	RGATCY	3 cut(s)	542, 957, 1690
PvuII	CAGCTG	2 cut(s)	1075, 1367
RsaI	GTAC	3 cut(s)	888, 1548, 1581
RsaNI	GTAC	3 cut(s)	887, 1547, 1580
RseI	CAYNNNNRTG	1 cut(s)	530
SalI	GTCGAC	1 cut(s)	982
SaqAI	TTAA	6 cut(s)	41, 54, 390, 479, 741, 1334
Sau3AI	GATC	7 cut(s)	542, 640, 957, 1147, 1520, 1636, 1690
Sau96I	GGNCC	6 cut(s)	132, 718, 818, 838, 1403, 1710
ScrFI	CCNGG	4 cut(s)	136, 560, 842, 1540
SduI	GDGCHC	2 cut(s)	469, 594
SexAI	ACCWGGT	1 cut(s)	840
SfaNI	GCATC	3 cut(s)	1048, 1351, 1574
SfcI	CTRYAG	3 cut(s)	579, 900, 1065
SinI	GGWCC	4 cut(s)	132, 818, 838, 1710
SmiMI	CAYNNNNRTG	1 cut(s)	530
SmlI	CTYRAG	4 cut(s)	53, 596, 705, 1676
SmoI	CTYRAG	4 cut(s)	53, 596, 705, 1676
SpeI	ACTAGT	1 cut(s)	461
SphI	GCATGC	2 cut(s)	251, 1327
Sse9I	AATT	9 cut(s)	42, 336, 566, 791, 927, 977, 1164, 1203, 1397
SsiI	CCGC	4 cut(s)	35, 203, 266, 317
SspI	AATATT	1 cut(s)	1132
SspMI	CTAG	4 cut(s)	398, 462, 1719, 1762
StyD4I	CCNGG	4 cut(s)	134, 558, 840, 1538
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	4 cut(s)	289, 506, 513, 580
TaiI	ACGT	4 cut(s)	22, 858, 1360, 1701
TaqI	TCGA	3 cut(s)	772, 983, 1619
TasI	AATT	9 cut(s)	42, 336, 566, 791, 927, 977, 1164, 1203, 1397
TfiI	GAWTC	5 cut(s)	148, 323, 647, 934, 989
Tru1I	TTAA	6 cut(s)	41, 54, 390, 479, 741, 1334
Tru9I	TTAA	6 cut(s)	41, 54, 390, 479, 741, 1334
TscAI	CASTG	4 cut(s)	166, 292, 1334, 1567
TseFI	GTSAC	1 cut(s)	1558
Tsp45I	GTSAC	1 cut(s)	1558
TspDTI	ATGAA	7 cut(s)	30, 75, 198, 211, 367, 420, 437
TspRI	CASTG	4 cut(s)	166, 292, 1334, 1567
Vha464I	CTTAAG	1 cut(s)	53
VneI	GTGCAC	2 cut(s)	465, 590
VpaK11BI	GGWCC	4 cut(s)	132, 818, 838, 1710
XapI	RAATTY	2 cut(s)	566, 1397
XceI	RCATGY	6 cut(s)	251, 303, 529, 1004, 1327, 1493
XmiI	GTMKAC	1 cut(s)	983
XmnI	GAANNNNTTC	4 cut(s)	66, 147, 189, 1260
XspI	CTAG	4 cut(s)	398, 462, 1719, 1762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.