MD13G1060300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010343
Physical Location & Seq
Reverse (-)
4229766 .. 4239510
9745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1060300.v1.1.491

Sequence Viewer

Length: 942 bp
ATGATTCGGAGGAGGAGCCTCGGCCTTGTACGGTGGCACAGTGCTCGCAGTTGCGTCGCCTCCTCTTCTTCTGCGTCGTTCAGAAGCCTCTCTTTCCTAACATCCCACCCCAAACTTCAAAAACCCTCTCTCCTCCTAAACCCTAAACCTCTTCCTCCTCATCCTTCTTCTTTGCCCTCTCTTTCTCTTAAACCAAATCCCAATATATTTCCTCCCGCTGCTCTACGTTTCGTAAGCACCAAATCCTCCGAGGATGTTGAGGAGGACATCCCAGATGATCGTTTTCCAGGTGACGAAATTGAAGAGGGAGACACCACAGATGGCTGGGAGGAAGAGGATGCCGGCGAGCCTGAGATTGGTGATGGAGGCGATGGTGGTGGGGTGGTGTTTCAGGGTGTCCCTTGGGGCGAGCGGGCTTTGTCCATTGCTCATGAGGTCTTAACACAATTCGAAGATAATGTGAAGCTCTTCTCTTTCAAGACTACTCCTCGAGGATATGTATATGTGAGACTGGACAAACTGTCAAACGAATACGGTTGTCCCGGCATGGAGGAACTTGAACGCTACAATCAAGAGTACAAGAAAAGACTAGATGAAGCTGGAGAACTTGGAGAAATACCTGAAAATTTGGCTCTTGAGGTGTCATCTCCTGGTGCGGAGAGGTTGCTAAAGGTACCGGATGATCTACAACGGTTTGAAGACATGCCTATGAGGGTATGCTATGCAGAAGAAGTGGATTCTAAATACCGAGAAAAGGACGGAGTGTTCGATCTGGAGTCCATTGAGGCAGAGTCAGAGAGCTGTGTGTGGAAGTTGGCAAATGTGAAGGCAAACAGGGACCCTAATAGCAAAGGCAGACCGTTAACACGTAAACAGCGGGATTGGAGATTAAAATTGCCATTTTCAGAGCATAAAAGGGTACTTCTGTATCTAGAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF150

Protein Analysis

314

Amino Acids

35.43

Weight (kDa)

5.02

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7912 PF25498 222 - 310 7e-39 Domain of unknown function (DUF7912)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69210 AT1G69210
fragaria_vesca FvH4_4g29740 FvH4_4g29740 FvH4_4g29740
malus_domestica MD13G1060300.v1.1
prunus_persica Prupe.1G289100_v2.0.a1 Prupe.1G289100_v2.0.a1
pyrus_communis pycom13g05370
rosa_chinensis RchiOBHm_Chr4g0438001
rosa_laevigata RLG00000006395
rosa_multiflora Rmu_sc0007538.1_g000005
rosa_roxburghii Rroxscaffold_5G00379150
rosa_rugosa Rorug04G0306100 Rorug04G0306200
rosa_samantha Rh4AG357800 Rh4BG368700 Rh4CG382800 Rh4DG363600
rosa_wichuraiana Rw0G011490 Rw0G016180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 489
Acc65I GGTACC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 673
AccBSI CCGCTC 1 cut(s) 412
AciI CCGC 4 cut(s) 216, 412, 656, 877
AcsI RAATTY 1 cut(s) 625
AfaI GTAC 5 cut(s) 30, 578, 675, 921, 938
AfiI CCNNNNNNNGG 2 cut(s) 356, 754
AflIII ACRYGT 1 cut(s) 866
AgsI TTSAA 5 cut(s) 119, 302, 478, 560, 698
AhdI GACNNNNNGTC 1 cut(s) 520
AjnI CCWGG 2 cut(s) 286, 649
AloI GAACNNNNNNTCC 2 cut(s) 749, 781
AluBI AGCT 3 cut(s) 466, 599, 801
AluI AGCT 3 cut(s) 466, 599, 801
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 303, 502
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 1 cut(s) 625
ArsI GACNNNNNNTTYG 2 cut(s) 749, 781
Asp700I GAANNNNTTC 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 838
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 302, 371
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 838
BaeI ACNNNNGTAYC 2 cut(s) 911, 911
BanI GGYRCC 1 cut(s) 673
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 205
BccI CCATC 3 cut(s) 314, 356, 365
BcgI CGANNNNNNTGC 4 cut(s) 26, 37, 60, 71
BciT130I CCWGG 2 cut(s) 288, 651
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 303, 502
BfaI CTAG 2 cut(s) 590, 932
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 3 cut(s) 288, 543, 651
Bme18I GGWCC 1 cut(s) 838
BmeRI GACNNNNNGTC 1 cut(s) 520
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 1 cut(s) 838
BmiI GGNNCC 4 cut(s) 17, 675, 839, 840
BmrFI CCNGG 3 cut(s) 288, 543, 651
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 2 cut(s) 621, 794
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 543
BsaAI YACGTR 1 cut(s) 869
BsaJI CCNNGG 3 cut(s) 19, 249, 401
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 754
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 516
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 288, 651
BseDI CCNNGG 3 cut(s) 19, 249, 401
BseGI GGATG 6 cut(s) 101, 160, 259, 267, 343, 685
BseLI CCNNNNNNNGG 2 cut(s) 356, 754
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 7 cut(s) 25, 28, 52, 122, 147, 275, 477
BseXI GCAGC 1 cut(s) 205
BseYI CCCAGC 1 cut(s) 324
BshFI GGCC 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 673
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 3 cut(s) 342, 543, 677
BslFI GGGAC 3 cut(s) 383, 525, 851
BslI CCNNNNNNNGG 2 cut(s) 356, 754
BsmAI GTCTC 2 cut(s) 303, 502
BsmFI GGGAC 3 cut(s) 383, 525, 851
BsnI GGCC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 489
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 3 cut(s) 277, 682, 769
BspACI CCGC 4 cut(s) 216, 412, 656, 877
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 4 cut(s) 17, 675, 839, 840
BspQI GCTCTTC 1 cut(s) 473
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 673
BsrBI CCGCTC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 19, 249, 401
BssMI GATC 3 cut(s) 277, 682, 769
BssT1I CCWWGG 1 cut(s) 401
Bst2UI CCWGG 2 cut(s) 288, 651
Bst4CI ACNGT 6 cut(s) 33, 41, 522, 536, 693, 861
Bst6I CTCTTC 5 cut(s) 70, 156, 297, 327, 473
BstBAI YACGTR 1 cut(s) 869
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 5 cut(s) 46, 343, 347, 410, 414
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 6 cut(s) 101, 160, 259, 267, 343, 685
BstKTI GATC 3 cut(s) 280, 685, 772
BstMAI GTCTC 2 cut(s) 303, 502
BstMBI GATC 3 cut(s) 277, 682, 769
BstNI CCWGG 2 cut(s) 288, 651
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 3 cut(s) 286, 541, 649
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 1 cut(s) 705
BsuRI GGCC 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 6 cut(s) 101, 160, 259, 267, 343, 685
BtsIMutI CAGTG 1 cut(s) 46
Cac8I GCNNGC 5 cut(s) 46, 343, 347, 410, 414
CciI TCATGA 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 838
CseI GACGC 2 cut(s) 43, 63
Csp6I GTAC 5 cut(s) 29, 577, 674, 920, 937
CviAII CATG 3 cut(s) 431, 547, 703
CviQI GTAC 5 cut(s) 29, 577, 674, 920, 937
DdeI CTNAG 1 cut(s) 351
DpnI GATC 3 cut(s) 279, 684, 771
DpnII GATC 3 cut(s) 277, 682, 769
DriI GACNNNNNGTC 1 cut(s) 520
Eam1104I CTCTTC 5 cut(s) 70, 156, 297, 327, 473
Eam1105I GACNNNNNGTC 1 cut(s) 520
EarI CTCTTC 5 cut(s) 70, 156, 297, 327, 473
Eco130I CCWWGG 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 838
Eco88I CYCGRG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 838
EcoRII CCWGG 2 cut(s) 286, 649
EcoT14I CCWWGG 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 401
FaeI CATG 3 cut(s) 434, 550, 706
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FaqI GGGAC 3 cut(s) 383, 525, 851
FatI CATG 3 cut(s) 430, 546, 702
FauI CCCGC 3 cut(s) 223, 405, 870
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 6 cut(s) 88, 147, 254, 266, 350, 692
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 2 cut(s) 590, 932
GluI GCNGC 1 cut(s) 219
GsaI CCCAGC 1 cut(s) 328
GsuI CTGGAG 2 cut(s) 621, 794
HaeIII GGCC 1 cut(s) 24
HapII CCGG 3 cut(s) 342, 543, 677
HgaI GACGC 2 cut(s) 43, 63
Hin1II CATG 3 cut(s) 434, 550, 706
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HinfI GANTC 4 cut(s) 4, 737, 776, 791
HpaI GTTAAC 1 cut(s) 864
HpaII CCGG 3 cut(s) 342, 543, 677
HphI GGTGA 2 cut(s) 302, 371
Hpy166II GTNNAC 2 cut(s) 864, 872
Hpy188I TCNGA 5 cut(s) 9, 83, 250, 796, 907
Hpy188III TCNNGA 6 cut(s) 431, 478, 572, 635, 773, 932
Hpy8I GTNNAC 2 cut(s) 864, 872
Hpy99I CGWCG 2 cut(s) 59, 79
HpyAV CCTTC 2 cut(s) 174, 820
HpyCH4III ACNGT 6 cut(s) 33, 41, 522, 536, 693, 861
HpyCH4IV ACGT 2 cut(s) 226, 868
HpyCH4V TGCA 1 cut(s) 725
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 2 cut(s) 226, 868
Hsp92II CATG 3 cut(s) 434, 550, 706
KflI GGGWCCC 1 cut(s) 838
KpnI GGTACC 1 cut(s) 677
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
KspAI GTTAAC 1 cut(s) 864
Kzo9I GATC 3 cut(s) 277, 682, 769
LguI GCTCTTC 1 cut(s) 473
LmnI GCTCC 1 cut(s) 15
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 590, 932
MaeII ACGT 2 cut(s) 226, 868
MaeIII GTNAC 1 cut(s) 290
MalI GATC 3 cut(s) 279, 684, 771
MbiI CCGCTC 1 cut(s) 412
MboI GATC 3 cut(s) 277, 682, 769
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 4 cut(s) 297, 446, 625, 893
MlyI GAGTC 2 cut(s) 785, 800
MroNI GCCGGC 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 4 cut(s) 189, 440, 863, 890
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 2 cut(s) 218, 877
MspI CCGG 3 cut(s) 342, 543, 677
MspR9I CCNGG 3 cut(s) 288, 543, 651
MvaI CCWGG 2 cut(s) 288, 651
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 543
NdeII GATC 3 cut(s) 277, 682, 769
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 3 cut(s) 434, 550, 706
NlaIV GGNNCC 4 cut(s) 17, 675, 839, 840
NmuCI GTSAC 1 cut(s) 290
NspI RCATGY 1 cut(s) 706
NspV TTCGAA 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 489
PagI TCATGA 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PdiI GCCGGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 4, 737
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 2 cut(s) 784, 799
PpsI GAGTC 2 cut(s) 784, 799
Ppu21I YACGTR 1 cut(s) 869
PpuMI RGGWCCY 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 2 cut(s) 286, 649
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 2 cut(s) 286, 649
PspN4I GGNNCC 4 cut(s) 17, 675, 839, 840
PspPI GGNCC 1 cut(s) 838
PspPPI RGGWCCY 1 cut(s) 838
PspXI VCTCGAGB 1 cut(s) 489
RsaI GTAC 5 cut(s) 30, 578, 675, 921, 938
RsaNI GTAC 5 cut(s) 29, 577, 674, 920, 937
RseI CAYNNNNRTG 1 cut(s) 707
SapI GCTCTTC 1 cut(s) 473
SaqAI TTAA 4 cut(s) 189, 440, 863, 890
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 3 cut(s) 277, 682, 769
Sau96I GGNCC 1 cut(s) 838
ScaI AGTACT 1 cut(s) 938
SchI GAGTC 2 cut(s) 785, 800
ScrFI CCNGG 3 cut(s) 288, 543, 651
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 1 cut(s) 838
SlaI CTCGAG 1 cut(s) 489
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 2 cut(s) 489, 635
SmoI CTYRAG 2 cut(s) 489, 635
Sse9I AATT 4 cut(s) 297, 446, 625, 893
SsiI CCGC 4 cut(s) 216, 412, 656, 877
SspMI CTAG 2 cut(s) 590, 932
StyD4I CCNGG 3 cut(s) 286, 541, 649
StyI CCWWGG 1 cut(s) 401
TaaI ACNGT 6 cut(s) 33, 41, 522, 536, 693, 861
TaiI ACGT 2 cut(s) 229, 871
TaqI TCGA 3 cut(s) 450, 490, 768
TasI AATT 4 cut(s) 297, 446, 625, 893
TatI WGTACW 2 cut(s) 576, 936
TfiI GAWTC 2 cut(s) 4, 737
Tru1I TTAA 4 cut(s) 189, 440, 863, 890
Tru9I TTAA 4 cut(s) 189, 440, 863, 890
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 838
XapI RAATTY 1 cut(s) 625
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 2 cut(s) 590, 932
ZrmI AGTACT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.