Rroxscaffold_5G00379150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010343
Physical Location & Seq
Reverse (-)
59633796 .. 59636262
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379150.1

Sequence Viewer

Length: 930 bp
ATGATGAACCTCCTCCTACAATTACACTCACAAACTGCTCGAAGGAGTATCACATTTTCTTTTTCTTCTTTCAGAAGCCTCTCTTTCTTATCAACCCACCCAATACAACAACCTCTTAAACCCTCTCAACACTTAAACCCTCCTCATTCTTCTTTGATTGGTTTCTCTCCTAAACCCAGACCCACAATATTTTCTCCCGCTACTCTTCGATTCCTAACAACCCAGTCCTCTGAGGACCTCGACGATTACCAAGATGACACCTTTCAAGGTGAAGAAACAGAAGAGGGAGGAACATCAGATGGATGGGAAGAAGGAGATAGTACAGATGGTTGGGAGGACGAGGATGTCGCCGAGCCTAAGGTTGGTGATGGAGGCGATGGTGGTGGAGTGGTGTTCCATGGTGTTCCCTGGGGTGAGCGGGCACTGTCCATTGCTCGTGAGGTGTTGACACAGTTTTGTGATGACATGAAGCTCTTCTCTTTCAAGACTACTCCTCGAGGCTATGTGTTTGTGAGACTGGACAAACTATCAAACGAATATGGATGTCCAAGTATGGAAGACCTGGGGCGCTACAGTAAAGAATACAAGAAAAGGTTGGATGAAGTTGGAGCACTTGGAGAAATACCCGAAGATTTGGCTGTTGAGGTATCATCTCCTGGTGCGGAGAGATTACTAAAGGTACCAGGTGATTTACCTCGGTTTAAAGACAAGACCATGAGAGTATGCTATTTAGAAGACGTGGATTCTAAACACCCAGAAAAGGATGGAGTATTTATGCTGGAGTCTGTTGAGACAGAGTCAGAGAGCTGTGTGTGGAAGTTGGCAAATGTGAAGGAAAACAGGGATCCGGACAGCAAAGGTGTCCTAGTTTGTGTTGCTGTCCTGTTTAGCTTTCTCGTGGACTTTGCCCTTGTCTCCTTCTGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF150

Protein Analysis

309

Amino Acids

34.57

Weight (kDa)

4.67

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7912 PF25498 224 - 287 7e-24 Domain of unknown function (DUF7912)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69210 AT1G69210
fragaria_vesca FvH4_4g29740 FvH4_4g29740 FvH4_4g29740
malus_domestica MD13G1060300.v1.1
prunus_persica Prupe.1G289100_v2.0.a1 Prupe.1G289100_v2.0.a1
pyrus_communis pycom13g05370
rosa_chinensis RchiOBHm_Chr4g0438001
rosa_laevigata RLG00000006395
rosa_multiflora Rmu_sc0007538.1_g000005
rosa_roxburghii Rroxscaffold_5G00379150
rosa_rugosa Rorug04G0306100 Rorug04G0306200
rosa_samantha Rh4AG357800 Rh4BG368700 Rh4CG382800 Rh4DG363600
rosa_wichuraiana Rw0G011490 Rw0G016180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AbsI CCTCGAGG 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 679
AccBSI CCGCTC 1 cut(s) 418
AccIII TCCGGA 1 cut(s) 847
AciI CCGC 3 cut(s) 198, 418, 662
AclWI GGATC 2 cut(s) 839, 852
AfaI GTAC 2 cut(s) 322, 681
AfiI CCNNNNNNNGG 2 cut(s) 362, 760
AgsI TTSAA 2 cut(s) 266, 484
AjiI CACGTC 1 cut(s) 739
AjnI CCWGG 4 cut(s) 407, 561, 655, 682
AluBI AGCT 3 cut(s) 472, 807, 891
AluI AGCT 3 cut(s) 472, 807, 891
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 3 cut(s) 508, 785, 919
AlwI GGATC 2 cut(s) 839, 852
Ama87I CYCGRG 1 cut(s) 495
Aor13HI TCCGGA 1 cut(s) 847
Asp700I GAANNNNTTC 1 cut(s) 473
Asp718I GGTACC 1 cut(s) 679
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 4 cut(s) 281, 377, 425, 698
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 235
AxyI CCTNAGG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 424
BamHI GGATCC 1 cut(s) 844
BanI GGYRCC 1 cut(s) 679
BauI CACGAG 2 cut(s) 435, 896
BbsI GAAGAC 2 cut(s) 564, 741
Bbv12I GWGCWC 1 cut(s) 613
BccI CCATC 6 cut(s) 293, 297, 320, 362, 371, 758
BciT130I CCWGG 4 cut(s) 409, 563, 657, 684
BcoDI GTCTC 3 cut(s) 508, 785, 919
BfaI CTAG 1 cut(s) 866
BfmI CTRYAG 1 cut(s) 571
BfoI RGCGCY 1 cut(s) 571
Bme1390I CCNGG 4 cut(s) 409, 563, 657, 684
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 739
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 681, 846
BmrFI CCNGG 4 cut(s) 409, 563, 657, 684
BmrI ACTGGG 1 cut(s) 217
BmuI ACTGGG 1 cut(s) 217
BpiI GAAGAC 2 cut(s) 564, 741
BpmI CTGGAG 1 cut(s) 800
BsaJI CCNNGG 5 cut(s) 397, 407, 408, 562, 695
BsaWI WCCGGW 1 cut(s) 847
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 760
Bse1I ACTGG 2 cut(s) 223, 522
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 847
BseBI CCWGG 4 cut(s) 409, 563, 657, 684
BseDI CCNNGG 5 cut(s) 397, 407, 408, 562, 695
BseGI GGATG 5 cut(s) 308, 349, 548, 604, 769
BseLI CCNNNNNNNGG 2 cut(s) 362, 760
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 223, 522
BseRI GAGGAG 2 cut(s) 132, 483
BseSI GKGCMC 1 cut(s) 424
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 1 cut(s) 848
BslI CCNNNNNNNGG 2 cut(s) 362, 760
BsmAI GTCTC 3 cut(s) 508, 785, 919
BsoBI CYCGRG 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 424, 613
Bsp13I TCCGGA 1 cut(s) 847
Bsp143I GATC 1 cut(s) 844
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 3 cut(s) 198, 418, 662
BspCNI CTCAG 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 847
BspLI GGNNCC 2 cut(s) 681, 846
BspPI GGATC 2 cut(s) 839, 852
BspQI GCTCTTC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 679
BsrBI CCGCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 2 cut(s) 223, 522
BssECI CCNNGG 5 cut(s) 397, 407, 408, 562, 695
BssMI GATC 1 cut(s) 844
BssSI CACGAG 2 cut(s) 435, 896
BssT1I CCWWGG 1 cut(s) 397
Bst2BI CACGAG 2 cut(s) 435, 896
Bst2UI CCWGG 4 cut(s) 409, 563, 657, 684
Bst4CI ACNGT 3 cut(s) 426, 453, 575
Bst6I CTCTTC 3 cut(s) 210, 276, 479
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 2 cut(s) 231, 357
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 5 cut(s) 308, 349, 548, 604, 769
BstH2I RGCGCY 1 cut(s) 571
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 3 cut(s) 508, 785, 919
BstMBI GATC 1 cut(s) 844
BstNI CCWGG 4 cut(s) 409, 563, 657, 684
BstSCI CCNGG 4 cut(s) 407, 561, 655, 682
BstSFI CTRYAG 1 cut(s) 571
BstSLI GKGCMC 1 cut(s) 424
BstV2I GAAGAC 2 cut(s) 564, 741
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
Bsu36I CCTNAGG 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 390
BtrI CACGTC 1 cut(s) 739
BtsCI GGATG 5 cut(s) 308, 349, 548, 604, 769
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 570
Cfr13I GGNCC 1 cut(s) 235
CsiI ACCWGGT 1 cut(s) 682
Csp6I GTAC 2 cut(s) 321, 680
CviAII CATG 3 cut(s) 398, 466, 715
CviJI RGCY 7 cut(s) 78, 355, 472, 501, 638, 807, 891
CviKI_1 RGCY 7 cut(s) 78, 355, 472, 501, 638, 807, 891
CviQI GTAC 2 cut(s) 321, 680
DdeI CTNAG 2 cut(s) 231, 357
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DraI TTTAAA 1 cut(s) 703
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
Eam1104I CTCTTC 3 cut(s) 210, 276, 479
EarI CTCTTC 3 cut(s) 210, 276, 479
Eco130I CCWWGG 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 235
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 4 cut(s) 407, 561, 655, 682
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 3 cut(s) 401, 469, 718
FaiI YATR 9 cut(s) 399, 467, 504, 540, 554, 716, 724, 776, 928
FalI AAGNNNNNCTT 2 cut(s) 67, 99
FatI CATG 3 cut(s) 397, 465, 714
FauI CCCGC 2 cut(s) 205, 411
FokI GGATG 5 cut(s) 315, 356, 555, 611, 776
FspBI CTAG 1 cut(s) 866
GlaI GCGC 1 cut(s) 569
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 571
HapII CCGG 1 cut(s) 848
HhaI GCGC 1 cut(s) 570
Hin1II CATG 3 cut(s) 401, 469, 718
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 4 cut(s) 210, 743, 782, 797
HpaII CCGG 1 cut(s) 848
HphI GGTGA 4 cut(s) 281, 377, 425, 698
Hpy166II GTNNAC 2 cut(s) 447, 901
Hpy188I TCNGA 4 cut(s) 74, 232, 298, 802
Hpy188III TCNNGA 3 cut(s) 437, 484, 848
Hpy8I GTNNAC 2 cut(s) 447, 901
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 36, 305, 826, 928
HpyCH4III ACNGT 3 cut(s) 426, 453, 575
HpyCH4IV ACGT 1 cut(s) 738
HpyF3I CTNAG 2 cut(s) 231, 357
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 3 cut(s) 401, 469, 718
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 1 cut(s) 847
KpnI GGTACC 1 cut(s) 683
Kzo9I GATC 1 cut(s) 844
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 1 cut(s) 608
MabI ACCWGGT 1 cut(s) 682
MaeI CTAG 1 cut(s) 866
MaeII ACGT 1 cut(s) 738
MalI GATC 1 cut(s) 846
MbiI CCGCTC 1 cut(s) 418
MboI GATC 1 cut(s) 844
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 2 cut(s) 424, 613
MluCI AATT 1 cut(s) 20
MlyI GAGTC 2 cut(s) 791, 806
MmeI TCCRAC 2 cut(s) 576, 586
MroI TCCGGA 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 3 cut(s) 117, 134, 702
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 4 cut(s) 409, 563, 657, 684
MvaI CCWGG 4 cut(s) 409, 563, 657, 684
NcoI CCATGG 1 cut(s) 397
NdeII GATC 1 cut(s) 844
NlaIII CATG 3 cut(s) 401, 469, 718
NlaIV GGNNCC 2 cut(s) 681, 846
NmeAIII GCCGAG 1 cut(s) 376
PaeR7I CTCGAG 1 cut(s) 495
PasI CCCWGGG 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 479
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 210, 743
PflFI GACNNNGTC 1 cut(s) 796
PleI GAGTC 2 cut(s) 790, 805
PpsI GAGTC 2 cut(s) 790, 805
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 4 cut(s) 407, 561, 655, 682
PspGI CCWGG 4 cut(s) 407, 561, 655, 682
PspN4I GGNNCC 2 cut(s) 681, 846
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 495
PsuI RGATCY 1 cut(s) 844
PsyI GACNNNGTC 1 cut(s) 796
RsaI GTAC 2 cut(s) 322, 681
RsaNI GTAC 2 cut(s) 321, 680
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 3 cut(s) 117, 134, 702
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 791, 806
ScrFI CCNGG 4 cut(s) 409, 563, 657, 684
SduI GDGCHC 2 cut(s) 424, 613
SexAI ACCWGGT 1 cut(s) 682
SfcI CTRYAG 1 cut(s) 571
Sfr274I CTCGAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 495
SmoI CTYRAG 1 cut(s) 495
Sse9I AATT 1 cut(s) 20
SsiI CCGC 3 cut(s) 198, 418, 662
SspI AATATT 1 cut(s) 189
SspMI CTAG 1 cut(s) 866
StyD4I CCNGG 4 cut(s) 407, 561, 655, 682
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 426, 453, 575
TaiI ACGT 1 cut(s) 741
TaqI TCGA 4 cut(s) 40, 208, 240, 496
TasI AATT 1 cut(s) 20
TatI WGTACW 1 cut(s) 320
TfiI GAWTC 2 cut(s) 210, 743
Tru1I TTAA 3 cut(s) 117, 134, 702
Tru9I TTAA 3 cut(s) 117, 134, 702
TscAI CASTG 1 cut(s) 429
TspDTI ATGAA 3 cut(s) 20, 482, 615
TspRI CASTG 1 cut(s) 429
Tth111I GACNNNGTC 1 cut(s) 796
VpaK11BI GGWCC 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.