RLG00000006395

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010343
Physical Location & Seq
Forward (+)
6731801 .. 6733607
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006395

Sequence Viewer

Length: 807 bp
ATGATGAACCTCCTCTTACAATTACACTCGCAAAGACCCACAATATTTTCTCCCGCTACTCCTCGATTCCTAACAACCCAGTCCTCTGAGGACCTCGACGATTACCAAGATGACACCTTTCAAGGTGAAGAAACAGAAGGGGGAGGAACATCAGATGGATGGGAAGAAGGAGATAGTACAGATGGTTGGGAGGACGAGGATGTCGCTGAGCCTAAGGTTGGTGATGGAGGCGATGGTGGTGGAGTGGTGTTCCATGGTGTTCCCTGGGGTGAGCGGGCACTGTCCATTGCTCGTGAGGTGTTGACACAGTTTTGTGATGACATGAAGCTCTTCTCTTTCAAGACTACTCCTCGAGGATATGTGTTTGTGAGACTGGACAAACTATCAAACGAATATGGATGTCCAAGTATGGAAGACCTTGGGCGCTACAGTAAAGAATACAAGAAAAGGTTGGATGAAGTTGGAGCACTTGGAGAAATACCCGAAGATTTGGCTGTTGAGGTATCATCTCCTGGTGCGGAGAGATTACTAAAGGTACCAGGTGATTTACCTCGGTTTAAAGACAAGACCATGAGAGTAAGCTATTTAGAAGATTTGGATTCTAAACACCCAGAAAAGGATGGAGTATTTATGCTGGAGTCTGTTGAGACAGAGTCAGAGAGCTGTGTTTGGAAGTTGGCAAATGTGAAGGAAAACAGGGATCCGGACAGCAAAGGTAGACCATTAACACGTAAACAGACAGATTGGAGATTAAAATTGCCATTTTCTGCGCATAAAAGGGTACTTCTGTACCTAAACTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF150

Protein Analysis

269

Amino Acids

30.17

Weight (kDa)

4.7

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7912 PF25498 176 - 264 9.6e-37 Domain of unknown function (DUF7912)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69210 AT1G69210
fragaria_vesca FvH4_4g29740 FvH4_4g29740 FvH4_4g29740
malus_domestica MD13G1060300.v1.1
prunus_persica Prupe.1G289100_v2.0.a1 Prupe.1G289100_v2.0.a1
pyrus_communis pycom13g05370
rosa_chinensis RchiOBHm_Chr4g0438001
rosa_laevigata RLG00000006395
rosa_multiflora Rmu_sc0007538.1_g000005
rosa_roxburghii Rroxscaffold_5G00379150
rosa_rugosa Rorug04G0306100 Rorug04G0306200
rosa_samantha Rh4AG357800 Rh4BG368700 Rh4CG382800 Rh4DG363600
rosa_wichuraiana Rw0G011490 Rw0G016180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AbsI CCTCGAGG 1 cut(s) 351
Acc16I TGCGCA 1 cut(s) 771
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 718
AccIII TCCGGA 1 cut(s) 703
AciI CCGC 3 cut(s) 54, 274, 518
AclWI GGATC 2 cut(s) 695, 708
AfaI GTAC 4 cut(s) 178, 537, 783, 791
AfiI CCNNNNNNNGG 2 cut(s) 218, 616
AflIII ACRYGT 1 cut(s) 728
AgsI TTSAA 2 cut(s) 122, 340
AjnI CCWGG 3 cut(s) 263, 511, 538
AluBI AGCT 3 cut(s) 328, 582, 663
AluI AGCT 3 cut(s) 328, 582, 663
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 2 cut(s) 364, 641
AlwI GGATC 2 cut(s) 695, 708
Ama87I CYCGRG 1 cut(s) 351
Aor13HI TCCGGA 1 cut(s) 703
Asp700I GAANNNNTTC 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 2 cut(s) 426, 772
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 4 cut(s) 137, 233, 281, 554
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 1 cut(s) 91
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 280
BaeI ACNNNNGTAYC 4 cut(s) 773, 773, 806, 806
BamHI GGATCC 1 cut(s) 700
BanI GGYRCC 1 cut(s) 535
BauI CACGAG 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 469
BccI CCATC 6 cut(s) 149, 153, 176, 218, 227, 614
BciT130I CCWGG 3 cut(s) 265, 513, 540
BcoDI GTCTC 2 cut(s) 364, 641
BfmI CTRYAG 1 cut(s) 427
BfoI RGCGCY 1 cut(s) 427
BlpI GCTNAGC 1 cut(s) 207
Bme1390I CCNGG 3 cut(s) 265, 513, 540
Bme18I GGWCC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 537, 702
BmrFI CCNGG 3 cut(s) 265, 513, 540
BmrI ACTGGG 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 656
Bpu1102I GCTNAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 784
BsaAI YACGTR 1 cut(s) 731
BsaJI CCNNGG 5 cut(s) 253, 263, 264, 418, 551
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 616
Bse1I ACTGG 2 cut(s) 79, 378
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 3 cut(s) 265, 513, 540
BseDI CCNNGG 5 cut(s) 253, 263, 264, 418, 551
BseGI GGATG 5 cut(s) 164, 205, 404, 460, 625
BseLI CCNNNNNNNGG 2 cut(s) 218, 616
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 78, 198
BseNI ACTGG 2 cut(s) 79, 378
BseRI GAGGAG 2 cut(s) 51, 339
BseSI GKGCMC 1 cut(s) 280
BshNI GGYRCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 1 cut(s) 704
BslI CCNNNNNNNGG 2 cut(s) 218, 616
BsmAI GTCTC 2 cut(s) 364, 641
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 280, 469
Bsp13I TCCGGA 1 cut(s) 703
Bsp143I GATC 1 cut(s) 700
Bsp1720I GCTNAGC 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 3 cut(s) 54, 274, 518
BspCNI CTCAG 2 cut(s) 79, 199
BspEI TCCGGA 1 cut(s) 703
BspLI GGNNCC 2 cut(s) 537, 702
BspPI GGATC 2 cut(s) 695, 708
BspQI GCTCTTC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 2 cut(s) 79, 378
BssECI CCNNGG 5 cut(s) 253, 263, 264, 418, 551
BssMI GATC 1 cut(s) 700
BssSI CACGAG 1 cut(s) 291
BssT1I CCWWGG 2 cut(s) 253, 418
Bst2BI CACGAG 1 cut(s) 291
Bst2UI CCWGG 3 cut(s) 265, 513, 540
Bst4CI ACNGT 3 cut(s) 282, 309, 431
Bst6I CTCTTC 1 cut(s) 335
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 3 cut(s) 87, 207, 213
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 5 cut(s) 164, 205, 404, 460, 625
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 426, 772
BstKTI GATC 1 cut(s) 703
BstMAI GTCTC 2 cut(s) 364, 641
BstMBI GATC 1 cut(s) 700
BstNI CCWGG 3 cut(s) 265, 513, 540
BstSCI CCNGG 3 cut(s) 263, 511, 538
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 280
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
Bsu36I CCTNAGG 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 5 cut(s) 164, 205, 404, 460, 625
BtsIMutI CAGTG 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 2 cut(s) 426, 772
Cfr13I GGNCC 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 538
Csp6I GTAC 4 cut(s) 177, 536, 782, 790
CviAII CATG 3 cut(s) 254, 322, 571
CviJI RGCY 5 cut(s) 211, 328, 494, 582, 663
CviKI_1 RGCY 5 cut(s) 211, 328, 494, 582, 663
CviQI GTAC 4 cut(s) 177, 536, 782, 790
DdeI CTNAG 3 cut(s) 87, 207, 213
DpnI GATC 1 cut(s) 702
DpnII GATC 1 cut(s) 700
DraI TTTAAA 1 cut(s) 559
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 253, 418
Eco47I GGWCC 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 3 cut(s) 263, 511, 538
EcoT14I CCWWGG 2 cut(s) 253, 418
ErhI CCWWGG 2 cut(s) 253, 418
FaeI CATG 3 cut(s) 257, 325, 574
FaiI YATR 8 cut(s) 255, 323, 360, 396, 410, 572, 632, 774
FatI CATG 3 cut(s) 253, 321, 570
FauI CCCGC 2 cut(s) 61, 267
FblI GTMKAC 1 cut(s) 718
FokI GGATG 5 cut(s) 171, 212, 411, 467, 632
FspI TGCGCA 1 cut(s) 771
GlaI GCGC 2 cut(s) 425, 771
GsuI CTGGAG 1 cut(s) 656
HaeII RGCGCY 1 cut(s) 427
HapII CCGG 1 cut(s) 704
HhaI GCGC 2 cut(s) 426, 772
Hin1II CATG 3 cut(s) 257, 325, 574
Hin6I GCGC 2 cut(s) 424, 770
HinP1I GCGC 2 cut(s) 424, 770
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 4 cut(s) 66, 599, 638, 653
HpaII CCGG 1 cut(s) 704
HphI GGTGA 4 cut(s) 137, 233, 281, 554
Hpy166II GTNNAC 3 cut(s) 303, 719, 734
Hpy188I TCNGA 3 cut(s) 88, 154, 658
Hpy188III TCNNGA 3 cut(s) 293, 340, 704
Hpy8I GTNNAC 3 cut(s) 303, 719, 734
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 3 cut(s) 131, 161, 682
HpyCH4III ACNGT 3 cut(s) 282, 309, 431
HpyCH4IV ACGT 1 cut(s) 730
HpyF3I CTNAG 3 cut(s) 87, 207, 213
HpySE526I ACGT 1 cut(s) 730
Hsp92II CATG 3 cut(s) 257, 325, 574
HspAI GCGC 2 cut(s) 424, 770
Kpn2I TCCGGA 1 cut(s) 703
KpnI GGTACC 1 cut(s) 539
Kzo9I GATC 1 cut(s) 700
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 1 cut(s) 464
MabI ACCWGGT 1 cut(s) 538
MaeII ACGT 1 cut(s) 730
MalI GATC 1 cut(s) 702
MbiI CCGCTC 1 cut(s) 274
MboI GATC 1 cut(s) 700
MboII GAAGA 6 cut(s) 140, 176, 322, 425, 497, 602
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 2 cut(s) 280, 469
MluCI AATT 2 cut(s) 20, 755
MlyI GAGTC 2 cut(s) 647, 662
MmeI TCCRAC 2 cut(s) 432, 442
MroI TCCGGA 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 3 cut(s) 558, 725, 752
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 3 cut(s) 265, 513, 540
MvaI CCWGG 3 cut(s) 265, 513, 540
NcoI CCATGG 1 cut(s) 253
NdeII GATC 1 cut(s) 700
NlaIII CATG 3 cut(s) 257, 325, 574
NlaIV GGNNCC 2 cut(s) 537, 702
NsbI TGCGCA 1 cut(s) 771
PaeR7I CTCGAG 1 cut(s) 351
PasI CCCWGGG 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 66, 599
PflFI GACNNNGTC 1 cut(s) 652
PleI GAGTC 2 cut(s) 646, 661
PpsI GAGTC 2 cut(s) 646, 661
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 3 cut(s) 263, 511, 538
PspGI CCWGG 3 cut(s) 263, 511, 538
PspN4I GGNNCC 2 cut(s) 537, 702
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 351
PsuI RGATCY 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 652
RsaI GTAC 4 cut(s) 178, 537, 783, 791
RsaNI GTAC 4 cut(s) 177, 536, 782, 790
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 3 cut(s) 558, 725, 752
Sau3AI GATC 1 cut(s) 700
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 2 cut(s) 647, 662
ScrFI CCNGG 3 cut(s) 265, 513, 540
SduI GDGCHC 2 cut(s) 280, 469
SexAI ACCWGGT 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 351
SinI GGWCC 1 cut(s) 91
SlaI CTCGAG 1 cut(s) 351
SmlI CTYRAG 2 cut(s) 351, 799
SmoI CTYRAG 2 cut(s) 351, 799
Sse9I AATT 2 cut(s) 20, 755
SsiI CCGC 3 cut(s) 54, 274, 518
SspI AATATT 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 263, 511, 538
StyI CCWWGG 2 cut(s) 253, 418
TaaI ACNGT 3 cut(s) 282, 309, 431
TaiI ACGT 1 cut(s) 733
TaqI TCGA 3 cut(s) 64, 96, 352
TasI AATT 2 cut(s) 20, 755
TatI WGTACW 1 cut(s) 176
TfiI GAWTC 2 cut(s) 66, 599
Tru1I TTAA 3 cut(s) 558, 725, 752
Tru9I TTAA 3 cut(s) 558, 725, 752
TscAI CASTG 1 cut(s) 285
TspDTI ATGAA 3 cut(s) 20, 338, 471
TspRI CASTG 1 cut(s) 285
Tth111I GACNNNGTC 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 91
XhoI CTCGAG 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.