MD13G1063500.v1.1

Chromatin modification-related protein EAF7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008569
Physical Location & Seq
Forward (+)
4394264 .. 4396345
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1063500.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGAAAGGAGTGGTGGCAAAGGCAGGGAAGAAGAGGAACAAGACGGCATGTCCGTACACTCTCCTTGCAAACCTCCCCCTTCCTCTGCTTCTTCTCTCCCAAAGGAGCAATCACAGGTTGAATTGGAGCTCAGACTGTTTGAAGCTCTTGAAATTTACCCTCCCGGAAAATTACAAGGCATACACCGTCATTTCGTGCTTTATGGTTTAATGGAATTTCTTCGTAGAAGCTTTGATCGACACTTCTCTGCTGACGAGGTTCTGCAATTGCTAGAGAGGTTCTACAACTTGGAAATGCTGAAACCAGACGACGAGGAGATGGATTTCCTGAATCGCGAGGAAGAGTTTTGCCTGCCTCAAAATTTCTTCGTCAAGGAAGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Eaf7

Protein Analysis

129

Amino Acids

15.06

Weight (kDa)

4.74

Isoelectric Point (pI)

78.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Eaf7 PF07904 43 - 106 3.2e-06 Chromatin modification-related protein EAF7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 336
AcsI RAATTY 3 cut(s) 153, 215, 361
AfaI GTAC 1 cut(s) 57
AgsI TTSAA 3 cut(s) 122, 143, 152
AhdI GACNNNNNGTC 1 cut(s) 49
AluBI AGCT 3 cut(s) 130, 146, 231
AluI AGCT 3 cut(s) 130, 146, 231
Alw21I GWGCWC 1 cut(s) 132
ApoI RAATTY 3 cut(s) 153, 215, 361
Asp700I GAANNNNTTC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 165
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 132
BccI CCATC 1 cut(s) 313
BceAI ACGGC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 165
BfaI CTAG 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 165
BmeRI GACNNNNNGTC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 1 cut(s) 235
Bsp68I TCGCGA 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 144
BspFNI CGCG 1 cut(s) 336
BssMI GATC 1 cut(s) 235
Bst4CI ACNGT 2 cut(s) 138, 188
Bst6I CTCTTC 2 cut(s) 27, 336
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 131
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 1 cut(s) 238
BstMBI GATC 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 163
BstUI CGCG 1 cut(s) 336
BtuMI TCGCGA 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 353
Csp6I GTAC 1 cut(s) 56
CviAII CATG 1 cut(s) 49
CviJI RGCY 3 cut(s) 130, 146, 231
CviKI_1 RGCY 3 cut(s) 130, 146, 231
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 1 cut(s) 131
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DriI GACNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 27, 336
Eam1105I GACNNNNNGTC 1 cut(s) 49
EarI CTCTTC 2 cut(s) 27, 336
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 132
FaeI CATG 1 cut(s) 52
FaiI YATR 4 cut(s) 50, 182, 204, 385
FatI CATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 1 cut(s) 272
HapII CCGG 1 cut(s) 165
Hin1II CATG 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 1 cut(s) 331
HpaII CCGG 1 cut(s) 165
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 3 cut(s) 149, 328, 335
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 90
HpyCH4III ACNGT 2 cut(s) 138, 188
HpyCH4V TGCA 2 cut(s) 69, 265
HpyF3I CTNAG 1 cut(s) 131
Hsp92II CATG 1 cut(s) 52
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 106, 127
LpnPI CCDG 6 cut(s) 10, 101, 178, 318, 341, 365
MaeI CTAG 1 cut(s) 272
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 6 cut(s) 41, 44, 84, 212, 353, 358
MfeI CAATTG 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 6 cut(s) 122, 153, 170, 215, 266, 361
MnlI CCTC 9 cut(s) 28, 84, 94, 171, 250, 270, 307, 331, 366
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 209
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 266
MvnI CGCG 1 cut(s) 336
NciI CCSGG 1 cut(s) 165
NdeII GATC 1 cut(s) 235
NlaIII CATG 1 cut(s) 52
NruI TCGCGA 1 cut(s) 336
NspI RCATGY 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 132
RruI TCGCGA 1 cut(s) 336
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 209
Sau3AI GATC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 132
SetI ASST 7 cut(s) 76, 120, 132, 148, 233, 261, 281
Sse9I AATT 6 cut(s) 122, 153, 170, 215, 266, 361
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 138, 188
TaqI TCGA 1 cut(s) 238
TasI AATT 6 cut(s) 122, 153, 170, 215, 266, 361
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TspGWI ACGGA 1 cut(s) 43
XapI RAATTY 3 cut(s) 153, 215, 361
XceI RCATGY 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.