Rorug04G0310500

Chromatin modification-related protein EAF7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008569
Physical Location & Seq
Forward (+)
48607655 .. 48610167
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0310500.1

Sequence Viewer

Length: 2118 bp
ATGGAGTCAGAGGATGATCATGTTTATATTCCTCAAGTAAAGGCAGAGTTGATGCCTAAATTGCACCAAGAGTTTGAAACAATAGATGATGTTGCTGCTTTCTACAATAGATATGCAAAAGAAGCAGGGTTTAGTATCAGAAGTCATTCTAGTGCAACCAACAAAGATAATACCCAACTTATGAGGAAGGAGTATGTCTGTTATAAGCAAGGAACTTCCAAAGTTGAAGGAGAAAAACGTAAGAGAGGATTACCAAAAGTGGGTTGTAAAGCAAGAATTGCAGTTGTGAGAAAGGAGTCTGGGAGATATGCAATCTCTGTATTTGTTGAGGGTCATAACCACCCATTAACAAGCCCACCTAGAGTACATTTGTTGAGATCTCATCGCCGTGTTTCAAAAGTTAACACAGTTCTATCACAACAACTAAGCTTGGTAAATGTTCAGAAACATACACAGTTTGAATTCTTTGGTGTTCAGGCAGGTGGCATTGAGAATATTGGTTGTACACAGCGTGATCTGTATAATTATGGAAGAACTTGTCGTGAAGCGAAGAAAGGGCATGATGGAGATCTATTGTACATGCATTTTCAGAATGAGAAAGAAAAAGATTCTTCTTTTGTCTATACAATAGAGTCAGATGAGGAAAACAGAGTAACAAGGTGCTTTTGGGCTGACTCAATTTCAAGACGAGCTTACAGCTTTTATGGAGATGTAGTTATCTTTGATACTACATACAACACAAATCGGTATGGAATGATTTTTGCACCATTCACTGGTGTTAATAATCATGGGCAGACAATCATCTTTGCTTGTGCATTCTTAAATGATGAGACAGCCGATTCTTTTGTTTGGTTATTCAAGGAACTTCTAAATGCTATGCCAGGAAATGCACCAGAAAATGCCCCCAAAATGCTTATTACTGACCAAGATCCTGCTATGACTAAAGCCATTTCAGAAGCACTCCCACAAACATTTCATAGATATTGCAGTTGGCACATTCTAAATAAATTTTCTGAGAAGCTAGATGCGATTAAATATCGGGATTACTACCAAGACTTTCATAGCTGCATATGGAATTCAAGTAGTAGAGAGGAGTTTGACTCAAGATGGATTGAAATTACTGAGAAGAGTGGGTTGAGTGATAACAAGTGGTTGGAGTCAATATATGAAATTCGTTCGTCATGGATACCAGCACATGTAAATCATGTTTTCTCAGCAGGAATGTCAAGTAGTCAAAGAGCGGAGAGTCAACATTCTTTCTTCAATCATGTTTTCTCAGCAGGAATGTCAAGTAGTCAAAGAGCGGAGAGTCAACATTCTTTCTTCAAGAATTATGTTTCTGATCAGAATTCGTTGGTGGAATTTATGGTTCAGTTTAAGAGGGGACTTCTTCACCAACGCCATTATGAGTTAGAGGAGGATCATATTAATATTGATGAGAAGCCAAAAACTGTCATGTCCCTTGACATAGAAGATCATATGGCTAAGGTTTATACACGTAAGCTATTTTATGAAGTTCAAGAACAATTGAAAGAGTGCTTCAAATATAAACTAGAACTTCTAAGAGAAAATGTCACTCATTGCGTGTTTAAGGTTATGCGAAAGAACATTGATACTTGTAGATCTCGTGAACTGACTTACGAAAAAGTTTCTGATTTTGCATCATGCAGCTGTAGAAAGTTTGAGACTGAAGGGCTTCCATGTCGGCATGTTTTGTCGTATTTGATTAAAATCCAAGATGTTGATAAATTGCCTATTCAGTACATCTTGAAGAGATGGACTAAAGCTGCAAGACAGAGTCTTGTGTTAGATTCTGATGGAATGGAGGTAAAAGATAACAAAGCTTTACTTGCAAGGAGGACGAAACTATTTCAGCATGCTACAGATGCAATTGATAAGGCAATGGTGAGTGATGAAGCTAGTCAGCTCTTTATGGAATGCTTGGATGCTTTCTTGGAGAATCTTAAAACATTGATTGGTAATGAAGCTAGAGAAAGTGCAGTAGTTTTGGAGACCAAGATTGATGCTATTCAGCATATTTTTAATGAACCAAATCAAGTGAGGGCCAAAGGGTGTGGGAGAAGGTTGAAAAAAAGGAAAAGAGAAGAGGAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Eaf7

Protein Analysis

705

Amino Acids

81.88

Weight (kDa)

7.83

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 34 - 117 8.4e-21 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 202 - 323 2e-08 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 238 - 335 4.4e-28 MULE transposase domain
SWIM PF04434 553 - 572 7.1e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AarI CACCTGC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 773
AccBSI CCGCTC 2 cut(s) 1241, 1304
AciI CCGC 2 cut(s) 1241, 1304
AclWI GGATC 2 cut(s) 923, 1428
AcsI RAATTY 6 cut(s) 461, 1007, 1075, 1170, 1348, 1361
AcuI CTGAAG 1 cut(s) 1710
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 4 cut(s) 366, 505, 578, 1763
AfiI CCNNNNNNNGG 2 cut(s) 260, 773
AflIII ACRYGT 2 cut(s) 1195, 1496
AjnI CCWGG 1 cut(s) 880
Alw26I GTCTC 3 cut(s) 824, 1679, 2006
AlwI GGATC 2 cut(s) 923, 1428
AoxI GGCC 1 cut(s) 2064
ApeKI GCWGC 4 cut(s) 95, 1065, 1668, 1787
ApoI RAATTY 6 cut(s) 461, 1007, 1075, 1170, 1348, 1361
AseI ATTAAT 1 cut(s) 1428
Asp700I GAANNNNTTC 2 cut(s) 145, 1695
AspS9I GGNCC 1 cut(s) 2064
AsuHPI GGTGA 2 cut(s) 1385, 1918
BauI CACGAG 1 cut(s) 1626
BbvI GCAGC 4 cut(s) 82, 1052, 1680, 1774
BccI CCATC 4 cut(s) 557, 1101, 1770, 1811
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 1 cut(s) 882
BciVI GTATCC 1 cut(s) 1179
BclI TGATCA 2 cut(s) 16, 1342
BcoDI GTCTC 3 cut(s) 824, 1679, 2006
BfaI CTAG 6 cut(s) 150, 360, 1022, 1553, 1920, 1989
BfmI CTRYAG 2 cut(s) 1672, 1881
BfuAI ACCTGC 1 cut(s) 470
BfuI GTATCC 1 cut(s) 1179
BglII AGATCT 3 cut(s) 377, 568, 1622
BisI GCNGC 4 cut(s) 96, 1066, 1669, 1788
BlsI GCNGC 4 cut(s) 97, 1067, 1670, 1789
Bme1390I CCNGG 1 cut(s) 882
BmgT120I GGNCC 1 cut(s) 2064
BmrFI CCNGG 1 cut(s) 882
BmsI GCATC 6 cut(s) 42, 1015, 1670, 1876, 1936, 2014
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
Bpu10I CCTNAGC 1 cut(s) 1485
BpuEI CTTGAG 2 cut(s) 18, 1087
BsaAI YACGTR 1 cut(s) 1499
BsaBI GATNNNNATC 2 cut(s) 567, 1730
BsaI GGTCTC 1 cut(s) 2006
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 773
Bse1I ACTGG 1 cut(s) 778
Bse3DI GCAATG 2 cut(s) 1579, 1908
Bse8I GATNNNNATC 2 cut(s) 567, 1730
BseBI CCWGG 1 cut(s) 882
BseGI GGATG 2 cut(s) 19, 1951
BseJI GATNNNNATC 2 cut(s) 567, 1730
BseLI CCNNNNNNNGG 2 cut(s) 260, 773
BseMI GCAATG 2 cut(s) 1579, 1908
BseMII CTCAG 4 cut(s) 1005, 1113, 1227, 1290
BseNI ACTGG 1 cut(s) 778
BseRI GAGGAG 2 cut(s) 1106, 1430
BseXI GCAGC 4 cut(s) 82, 1052, 1680, 1774
BsgI GTGCAG 1 cut(s) 2019
BshFI GGCC 1 cut(s) 2066
BslFI GGGAC 2 cut(s) 1398, 1444
BslI CCNNNNNNNGG 2 cut(s) 260, 773
BsmAI GTCTC 3 cut(s) 824, 1679, 2006
BsmFI GGGAC 2 cut(s) 1398, 1444
BsmI GAATGC 2 cut(s) 815, 1943
BsnI GGCC 1 cut(s) 2066
Bso31I GGTCTC 1 cut(s) 2006
Bsp1407I TGTACA 2 cut(s) 503, 576
Bsp143I GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
BspACI CCGC 2 cut(s) 1241, 1304
BspANI GGCC 1 cut(s) 2066
BspCNI CTCAG 4 cut(s) 1006, 1114, 1226, 1289
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 2 cut(s) 923, 1428
BspTNI GGTCTC 1 cut(s) 2006
BsrBI CCGCTC 2 cut(s) 1241, 1304
BsrDI GCAATG 2 cut(s) 1579, 1908
BsrGI TGTACA 2 cut(s) 503, 576
BsrI ACTGG 1 cut(s) 778
BssMI GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
BssSI CACGAG 1 cut(s) 1626
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 3 cut(s) 409, 456, 1453
Bst6I CTCTTC 3 cut(s) 1121, 1766, 2100
BstAPI GCANNNNNTGC 1 cut(s) 278
BstAUI TGTACA 2 cut(s) 503, 576
BstBAI YACGTR 1 cut(s) 1499
BstC8I GCNNGC 1 cut(s) 1878
BstDEI CTNAG 7 cut(s) 425, 1014, 1122, 1213, 1276, 1485, 1562
BstF5I GGATG 2 cut(s) 19, 1951
BstKTI GATC 9 cut(s) 19, 380, 517, 571, 931, 1345, 1423, 1477, 1625
BstMAI GTCTC 3 cut(s) 824, 1679, 2006
BstMBI GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
BstMWI GCNNNNNNNGC 5 cut(s) 61, 122, 278, 1850, 1886
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 4 cut(s) 583, 1199, 1712, 1880
BstSCI CCNGG 1 cut(s) 880
BstSFI CTRYAG 2 cut(s) 1672, 1881
BstV1I GCAGC 4 cut(s) 82, 1052, 1680, 1774
BstX2I RGATCY 4 cut(s) 377, 568, 928, 1622
BstYI RGATCY 4 cut(s) 377, 568, 928, 1622
BsuI GTATCC 1 cut(s) 1179
BsuRI GGCC 1 cut(s) 2066
BtgZI GCGATG 1 cut(s) 368
BtsCI GGATG 2 cut(s) 19, 1951
BtsIMutI CAGTG 1 cut(s) 771
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 1878
Cfr13I GGNCC 1 cut(s) 2064
Csp6I GTAC 4 cut(s) 365, 504, 577, 1762
CspCI CAANNNNNGTGG 2 cut(s) 2056, 2091
CviQI GTAC 4 cut(s) 365, 504, 577, 1762
DdeI CTNAG 7 cut(s) 425, 1014, 1122, 1213, 1276, 1485, 1562
DpnI GATC 9 cut(s) 18, 379, 516, 570, 930, 1344, 1422, 1476, 1624
DpnII GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 3 cut(s) 1121, 1766, 2100
EarI CTCTTC 3 cut(s) 1121, 1766, 2100
Eco31I GGTCTC 1 cut(s) 2006
Eco57I CTGAAG 1 cut(s) 1710
EcoRI GAATTC 3 cut(s) 461, 1075, 1348
EcoRII CCWGG 1 cut(s) 880
EcoT22I ATGCAT 1 cut(s) 585
FalI AAGNNNNNCTT 4 cut(s) 676, 708, 1833, 1865
FaqI GGGAC 2 cut(s) 1398, 1444
FauNDI CATATG 2 cut(s) 1070, 1479
FbaI TGATCA 2 cut(s) 16, 1342
Fnu4HI GCNGC 4 cut(s) 96, 1066, 1669, 1788
FokI GGATG 2 cut(s) 26, 1958
Fsp4HI GCNGC 4 cut(s) 96, 1066, 1669, 1788
FspBI CTAG 6 cut(s) 150, 360, 1022, 1553, 1920, 1989
GluI GCNGC 4 cut(s) 96, 1066, 1669, 1788
HaeIII GGCC 1 cut(s) 2066
HincII GTYRAC 3 cut(s) 403, 1250, 1313
HindII GTYRAC 3 cut(s) 403, 1250, 1313
HindIII AAGCTT 2 cut(s) 427, 1842
HpaI GTTAAC 1 cut(s) 403
HphI GGTGA 2 cut(s) 1385, 1918
Hpy166II GTNNAC 5 cut(s) 403, 506, 1250, 1313, 1631
Hpy188III TCNNGA 8 cut(s) 542, 684, 1040, 1104, 1327, 1520, 1628, 1768
Hpy8I GTNNAC 5 cut(s) 403, 506, 1250, 1313, 1631
HpyAV CCTTC 4 cut(s) 181, 221, 1685, 2076
HpyCH4III ACNGT 3 cut(s) 409, 456, 1453
HpyCH4IV ACGT 2 cut(s) 238, 1498
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 122, 278, 1850, 1886
HpyF3I CTNAG 7 cut(s) 425, 1014, 1122, 1213, 1276, 1485, 1562
HpySE526I ACGT 2 cut(s) 238, 1498
Ksp22I TGATCA 2 cut(s) 16, 1342
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
Lsp1109I GCAGC 4 cut(s) 82, 1052, 1680, 1774
LweI GCATC 6 cut(s) 42, 1015, 1670, 1876, 1936, 2014
MaeI CTAG 6 cut(s) 150, 360, 1022, 1553, 1920, 1989
MaeII ACGT 2 cut(s) 238, 1498
MaeIII GTNAC 2 cut(s) 652, 1573
MalI GATC 9 cut(s) 18, 379, 516, 570, 930, 1344, 1422, 1476, 1624
MbiI CCGCTC 2 cut(s) 1241, 1304
MboI GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
MfeI CAATTG 2 cut(s) 1526, 1890
MflI RGATCY 4 cut(s) 377, 568, 928, 1622
MlyI GAGTC 9 cut(s) 14, 305, 641, 668, 1094, 1166, 1255, 1318, 1807
MmeI TCCRAC 1 cut(s) 1134
Mph1103I ATGCAT 1 cut(s) 585
MroXI GAANNNNTTC 2 cut(s) 145, 1695
MslI CAYNNNNRTG 2 cut(s) 150, 387
MspA1I CMGCKG 1 cut(s) 1671
MspR9I CCNGG 1 cut(s) 882
MunI CAATTG 2 cut(s) 1526, 1890
Mva1269I GAATGC 2 cut(s) 815, 1943
MvaI CCWGG 1 cut(s) 882
MwoI GCNNNNNNNGC 5 cut(s) 61, 122, 278, 1850, 1886
NdeI CATATG 2 cut(s) 1070, 1479
NdeII GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
NmuCI GTSAC 1 cut(s) 1573
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 4 cut(s) 583, 1199, 1712, 1880
PaeI GCATGC 1 cut(s) 1880
PaqCI CACCTGC 1 cut(s) 470
PciI ACATGT 1 cut(s) 1195
PctI GAATGC 2 cut(s) 815, 1943
PdmI GAANNNNTTC 2 cut(s) 145, 1695
PfeI GAWTC 4 cut(s) 608, 839, 1811, 1960
PflFI GACNNNGTC 1 cut(s) 1797
PflMI CCANNNNNTGG 1 cut(s) 773
PkrI GCNGC 4 cut(s) 97, 1067, 1670, 1789
PleI GAGTC 9 cut(s) 13, 304, 640, 668, 1094, 1165, 1254, 1317, 1806
PpsI GAGTC 9 cut(s) 13, 304, 640, 668, 1094, 1165, 1254, 1317, 1806
Ppu21I YACGTR 1 cut(s) 1499
PscI ACATGT 1 cut(s) 1195
PshBI ATTAAT 1 cut(s) 1428
PsiI TTATAA 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspPI GGNCC 1 cut(s) 2064
PsuI RGATCY 4 cut(s) 377, 568, 928, 1622
PsyI GACNNNGTC 1 cut(s) 1797
PvuII CAGCTG 1 cut(s) 1671
RsaI GTAC 4 cut(s) 366, 505, 578, 1763
RsaNI GTAC 4 cut(s) 365, 504, 577, 1762
RseI CAYNNNNRTG 2 cut(s) 150, 387
SatI GCNGC 4 cut(s) 96, 1066, 1669, 1788
Sau3AI GATC 9 cut(s) 16, 377, 514, 568, 928, 1342, 1420, 1474, 1622
Sau96I GGNCC 1 cut(s) 2064
SchI GAGTC 9 cut(s) 14, 305, 641, 668, 1094, 1166, 1255, 1318, 1807
ScrFI CCNGG 1 cut(s) 882
SfaNI GCATC 6 cut(s) 42, 1015, 1670, 1876, 1936, 2014
SfcI CTRYAG 2 cut(s) 1672, 1881
SmiMI CAYNNNNRTG 2 cut(s) 150, 387
SmlI CTYRAG 2 cut(s) 33, 1102
SmoI CTYRAG 2 cut(s) 33, 1102
SphI GCATGC 1 cut(s) 1880
SsiI CCGC 2 cut(s) 1241, 1304
SspI AATATT 2 cut(s) 496, 1432
SspMI CTAG 6 cut(s) 150, 360, 1022, 1553, 1920, 1989
StyD4I CCNGG 1 cut(s) 880
TaaI ACNGT 3 cut(s) 409, 456, 1453
TaiI ACGT 2 cut(s) 241, 1501
TatI WGTACW 4 cut(s) 364, 503, 576, 1761
TfiI GAWTC 4 cut(s) 608, 839, 1811, 1960
TscAI CASTG 1 cut(s) 778
TseFI GTSAC 1 cut(s) 1573
TseI GCWGC 4 cut(s) 95, 1065, 1668, 1787
Tsp45I GTSAC 1 cut(s) 1573
TspDTI ATGAA 7 cut(s) 965, 1049, 1182, 1527, 1929, 1998, 2061
TspRI CASTG 1 cut(s) 778
Tth111I GACNNNGTC 1 cut(s) 1797
Van91I CCANNNNNTGG 1 cut(s) 773
VspI ATTAAT 1 cut(s) 1428
XapI RAATTY 6 cut(s) 461, 1007, 1075, 1170, 1348, 1361
XceI RCATGY 4 cut(s) 583, 1199, 1712, 1880
XmnI GAANNNNTTC 2 cut(s) 145, 1695
XspI CTAG 6 cut(s) 150, 360, 1022, 1553, 1920, 1989
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.