Rorug04G0311000

Chromatin modification-related protein EAF7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008569
Physical Location & Seq
Reverse (-)
48643746 .. 48647475
3730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0311000.1

Sequence Viewer

Length: 645 bp
ATGTCTTGTTTGACAGCCAGAACAGGGCGGCAGAGACAGCGGTACGAGGATCAATTGAGGCTTGTTTCAGGATGTATACCTTATAGATTTGAAGAGACGGTAGGGAGTAGCAATTGCAATGTAGAGAAAAATTTGCTTGTTCTCATGATTTCTTCACCAGATCGAGATGATCTTGTCTTTCCAAAGGGAGGATGGGAGAATGACGAAACCATGCATGAAGCTGCTTGTCGTGAGGCCTTAGAAGAAGCAGGAGTGAAGGGAGTTCTTGGTGAAACTCCACTAGGACAATGGGAGTTCAGAAGCAAGAGCAAACAGAACAATGGCAGCCTGCAAGGTGGTTGTAGAGGTTACATGTTTGCACTAAGGGTTACTGAAGAGCTCGACTCCTGGCCCGAGCAGGCCATCTACGGAAGGAAATGGGTTACCGTAGAAGACGCATTCAAATTCTGTCGGTATGACTGGATGCGAGAAGCTCTTAGAAAGCTTTTGATAGCACTCTCAGATAACACGGAAAATGGCCCGAGAGAAGAACTGGATGGACTTCCTTTGAGGCCAGTTATCTCAGAAGTCGGATCAGAGCATCAAATATCATCACCATCCGGTTGCTTTGCTAGTCATTCTAGTGTTCAGCAGCTCGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Eaf7

Protein Analysis

214

Amino Acids

24.08

Weight (kDa)

5.15

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 161 3.1e-11 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 76
AciI CCGC 2 cut(s) 28, 40
AclWI GGATC 2 cut(s) 57, 580
AcsI RAATTY 2 cut(s) 130, 443
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 2 cut(s) 24, 188
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 2 cut(s) 92, 442
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 6 cut(s) 221, 379, 473, 484, 634, 641
AluI AGCT 6 cut(s) 221, 379, 473, 484, 634, 641
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 28, 89
AlwI GGATC 2 cut(s) 57, 580
Ama87I CYCGRG 2 cut(s) 392, 520
AoxI GGCC 5 cut(s) 234, 389, 399, 517, 551
ApeKI GCWGC 4 cut(s) 221, 324, 631, 638
ApoI RAATTY 2 cut(s) 130, 443
AspS9I GGNCC 2 cut(s) 390, 518
AsuHPI GGTGA 3 cut(s) 147, 281, 585
AvaI CYCGRG 2 cut(s) 392, 520
BanII GRGCYC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 208, 336
BccI CCATC 4 cut(s) 186, 410, 530, 604
BciT130I CCWGG 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 28, 89
BfaI CTAG 3 cut(s) 281, 612, 621
BisI GCNGC 5 cut(s) 29, 222, 325, 632, 639
BlsI GCNGC 5 cut(s) 30, 223, 326, 633, 640
Bme1390I CCNGG 1 cut(s) 388
BmeT110I CYCGRG 2 cut(s) 392, 520
BmgT120I GGNCC 2 cut(s) 390, 518
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 2 cut(s) 453, 589
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BsaWI WCCGGW 1 cut(s) 599
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 188
Bse1I ACTGG 3 cut(s) 464, 537, 554
Bse3DI GCAATG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 388
BseGI GGATG 5 cut(s) 77, 197, 468, 541, 596
BseLI CCNNNNNNNGG 2 cut(s) 24, 188
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 513, 576
BseNI ACTGG 3 cut(s) 464, 537, 554
BseXI GCAGC 2 cut(s) 208, 336
BshFI GGCC 5 cut(s) 236, 391, 401, 519, 553
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 392, 520
BsiSI CCGG 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 24, 188
BsmAI GTCTC 2 cut(s) 28, 89
BsmBI CGTCTC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 5 cut(s) 236, 391, 401, 519, 553
BsoBI CYCGRG 2 cut(s) 392, 520
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 4 cut(s) 49, 160, 169, 572
BspACI CCGC 2 cut(s) 28, 40
BspANI GGCC 5 cut(s) 236, 391, 401, 519, 553
BspCNI CTCAG 2 cut(s) 512, 575
BspHI TCATGA 1 cut(s) 144
BspPI GGATC 2 cut(s) 57, 580
BspQI GCTCTTC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 124
BsrI ACTGG 3 cut(s) 464, 537, 554
BssMI GATC 4 cut(s) 49, 160, 169, 572
BssNAI GTATAC 1 cut(s) 77
Bst1107I GTATAC 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 100, 427
Bst6I CTCTTC 2 cut(s) 87, 369
BstC8I GCNNGC 3 cut(s) 329, 399, 636
BstDEI CTNAG 5 cut(s) 238, 362, 476, 499, 562
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 5 cut(s) 77, 197, 468, 541, 596
BstKTI GATC 4 cut(s) 52, 163, 172, 575
BstMAI GTCTC 2 cut(s) 28, 89
BstMBI GATC 4 cut(s) 49, 160, 169, 572
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 355
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 386
BstV1I GCAGC 2 cut(s) 208, 336
BstV2I GAAGAC 1 cut(s) 438
BstZ17I GTATAC 1 cut(s) 77
BsuRI GGCC 5 cut(s) 236, 391, 401, 519, 553
BtsCI GGATG 5 cut(s) 77, 197, 468, 541, 596
Cac8I GCNNGC 3 cut(s) 329, 399, 636
CciI TCATGA 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 390, 518
CseI GACGC 1 cut(s) 443
Csp6I GTAC 1 cut(s) 43
CviAII CATG 4 cut(s) 145, 211, 215, 352
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 5 cut(s) 238, 362, 476, 499, 562
DpnI GATC 4 cut(s) 51, 162, 171, 574
DpnII GATC 4 cut(s) 49, 160, 169, 572
Eam1104I CTCTTC 2 cut(s) 87, 369
EarI CTCTTC 2 cut(s) 87, 369
Ecl136II GAGCTC 1 cut(s) 379
Eco147I AGGCCT 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 381
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 393
Eco88I CYCGRG 2 cut(s) 392, 520
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 381
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 4 cut(s) 148, 214, 218, 355
FaiI YATR 7 cut(s) 77, 84, 146, 212, 216, 353, 456
FatI CATG 4 cut(s) 144, 210, 214, 351
FblI GTMKAC 1 cut(s) 76
Fnu4HI GCNGC 5 cut(s) 29, 222, 325, 632, 639
FokI GGATG 5 cut(s) 84, 204, 475, 548, 583
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 5 cut(s) 29, 222, 325, 632, 639
FspBI CTAG 3 cut(s) 281, 612, 621
GluI GCNGC 5 cut(s) 29, 222, 325, 632, 639
HaeIII GGCC 5 cut(s) 236, 391, 401, 519, 553
HapII CCGG 1 cut(s) 600
HgaI GACGC 1 cut(s) 443
Hin1II CATG 4 cut(s) 148, 214, 218, 355
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 1 cut(s) 383
HpaII CCGG 1 cut(s) 600
HphI GGTGA 3 cut(s) 147, 281, 585
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 5 cut(s) 299, 502, 565, 572, 577
Hpy188III TCNNGA 4 cut(s) 69, 145, 164, 230
Hpy8I GTNNAC 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 250, 405
HpyCH4III ACNGT 2 cut(s) 100, 427
HpyCH4V TGCA 4 cut(s) 117, 214, 331, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 5 cut(s) 238, 362, 476, 499, 562
Hsp92II CATG 4 cut(s) 148, 214, 218, 355
Kzo9I GATC 4 cut(s) 49, 160, 169, 572
LguI GCTCTTC 1 cut(s) 369
Lsp1109I GCAGC 2 cut(s) 208, 336
LweI GCATC 2 cut(s) 453, 589
MaeI CTAG 3 cut(s) 281, 612, 621
MaeIII GTNAC 3 cut(s) 347, 367, 421
MalI GATC 4 cut(s) 51, 162, 171, 574
MboI GATC 4 cut(s) 49, 160, 169, 572
MboII GAAGA 6 cut(s) 104, 144, 254, 386, 443, 539
MfeI CAATTG 2 cut(s) 53, 112
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 4 cut(s) 53, 112, 130, 443
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 6 cut(s) 40, 51, 182, 226, 338, 543
Mph1103I ATGCAT 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 1 cut(s) 600
MspR9I CCNGG 1 cut(s) 388
MunI CAATTG 2 cut(s) 53, 112
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeII GATC 4 cut(s) 49, 160, 169, 572
NlaIII CATG 4 cut(s) 148, 214, 218, 355
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 355
PagI TCATGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 236
PciI ACATGT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 1 cut(s) 437
PkrI GCNGC 5 cut(s) 30, 223, 326, 633, 640
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
PscI ACATGT 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 386
PspPI GGNCC 2 cut(s) 390, 518
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 1 cut(s) 381
SapI GCTCTTC 1 cut(s) 369
SatI GCNGC 5 cut(s) 29, 222, 325, 632, 639
Sau3AI GATC 4 cut(s) 49, 160, 169, 572
Sau96I GGNCC 2 cut(s) 390, 518
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 1 cut(s) 381
SetI ASST 9 cut(s) 82, 223, 337, 349, 381, 475, 486, 636, 643
SfaNI GCATC 2 cut(s) 453, 589
SmiMI CAYNNNNRTG 1 cut(s) 621
Sse9I AATT 4 cut(s) 53, 112, 130, 443
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 2 cut(s) 28, 40
SspMI CTAG 3 cut(s) 281, 612, 621
SstI GAGCTC 1 cut(s) 381
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 386
TaaI ACNGT 2 cut(s) 100, 427
TaqI TCGA 2 cut(s) 163, 381
TasI AATT 4 cut(s) 53, 112, 130, 443
TauI GCSGC 1 cut(s) 31
TseI GCWGC 4 cut(s) 221, 324, 631, 638
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 2 cut(s) 423, 524
XapI RAATTY 2 cut(s) 130, 443
XceI RCATGY 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 2 cut(s) 189, 285
XmiI GTMKAC 1 cut(s) 76
XspI CTAG 3 cut(s) 281, 612, 621
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.