Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007326

Physical Location & Seq

Genomic Coordinates Forward (+)

4637048 .. 4640408

Gene Structure 3361 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1067600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGGCTGACAACCAAGAGCAACCGCCCACGCCGCCGTCCGGACCATCGCAACCGTCGAAGCCCGCAGCGACTCATCATCCTCCCCGCCCCACGATCACTCTCCCGCAGCGCACCTCTTTCGAATCAATCTTCAGCTCCGCCGGCGGCGGGAATACTGGGCCCGGTCTGGGCCTGGGGTTCAGCCCCGGACCCATGACTCTCGTCTCCAACTTCTTCTCCGACGGCGGCGACGACTGCAAGTCCTTCTCCCAGCTGCTCGCCGGAGCCATGACGTCTCCGTCGCCGAGGGCGCTGGGGTTCCCGCAGCTCGAGGAGCGAAATTCCGGCGACGGCGATGGAAGTTCGGATTTCCGGTTCAAGCAGAACAGACCGCCGCCTATGTTTTCGGTCCCGACGCCGTCCGGGTTTCTCGACTCGCCGGGAATCTTCTCACCCGGTCAGGGACCCTTTGGAATGACACACCAGCAGGCACTAGCACAGGTCACGGCTCAGGCTGCTGCACTCTCCCCATCCTTTTTCAATTTCTCAACTGAGCATTCAACATCTTTCTCAACAGCACCCGCCACAGTGTTGACACAGCCTCCATCCTTTACTTCCGATACAACACCACCACAAGAGATACCGTCTGGAACACCTGACTTTGCCGTTGCGACAAAAGAATCATCTGACATTTCCCTTTCAGATCAGAGATCCCAACTGTCTTCATTTACTGTCGATAAGCCTAATGATGATGGTTACAATTGGCGGAAGTATGGACAGAAGCAGGTGAAAGGTAGTGAATTTCCTCGAAGTTATTACAAGTGCACCCATCCAAATTGTCCAGTAAAGAAGAAGGTTGAACGCTCGATTGAAGGACAAATAACTGAAATTATTTACAGGGGCGAGCATAATCATGAACGTCATCAGTCCAAGCGTCCCCTCAATGGAAATAGTCAGGCAAATTCCGACTTAGCTTCCCAAGTTCATGGTGGGAATTTGAACAAACCAAAAGAAGGGACAGGTCCTTATTCAATGTCTAAGAAGCGTCAGGAATCCAGCCAAGCAACACATGATAATTTATCTGGGACAAGTGACAGTGAGGAAGTAGAAGATGCGGAAACCAGAGTTGATGAGAAAGATGAAGATCAACCTGACCCCAAGAGAAGAAACACCGAGGTCAGGTCAGAAGCAGCGGCAGCTTCTACACATCGGACTGTTACAGAGCCTAGAATCATAGTACAGACAACCAGTGAAGTCGACCTCTTAGACGATGGTTACAGGTGGCGAAAGTATGGGCAAAAAGTCGTCAAAGGCAATCCTTATCCACGGAGCTATTACAAATGCACGTTCCCTGCATGTAACGTCCGTAAGCACGTAGAGAGAGCCTCAACAGATCCTAAGGCTGTCATAACAACATATGAGGGCAAGCATAATCATGATGTACCAGCTTCTAAAACCAGCAGCCACTACACAGGCAACAACAATGCATCGATTTTAAGATCAACGAATCCAGGAAATGGGAAGGTTAACCAGATGGATCTTAGAAACAGGGACCAGCAGCCTATAGCAAGTTTACGTTTAAAAGGAGAACAAATAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009753 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043901 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0140110 GO:1900150 GO:1900424 GO:1900425 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

527

Amino Acids

57.34

Weight (kDa)

7.26

Isoelectric Point (pI)

52.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	243 - 299	8.2e-28	WRKY DNA -binding domain
WRKY	PF03106	416 - 473	1.7e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13960 AT1G13960 AT2G03340
fragaria_vesca	FvH4_4g30640 FvH4_4g30640 FvH4_4g30640 FvH4_4g30640
malus_domestica	MD13G1067600.v1.1 MD16G1066500.v1.1
prunus_persica	Prupe.1G280700_v2.0.a1 Prupe.1G280700_v2.0.a1
pyrus_communis	pycom13g05940 pycom16g05790
rosa_chinensis	RchiOBHm_Chr4g0439041
rosa_laevigata	RLG00000006307
rosa_multiflora	Rmu_sc0001338.1_g000004 Rmu_sc0003226.1_g000090
rosa_roxburghii	Rroxscaffold_5G00380040
rosa_rugosa	Rorug04G0315200
rosa_samantha	Rh4AG365900 Rh4BG377200 Rh4CG391300 Rh4DG371900
rosa_wichuraiana	Rw0G007420 Rw0G016970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	754
AasI	GACNNNNNNGTC	1 cut(s)	277
AatII	GACGTC	1 cut(s)	275
Acc36I	ACCTGC	1 cut(s)	754
AccB7I	CCANNNNNTGG	1 cut(s)	1496
AccI	GTMKAC	1 cut(s)	1236
AccIII	TCCGGA	1 cut(s)	38
AclWI	GGATC	3 cut(s)	684, 1367, 1524
AcsI	RAATTY	4 cut(s)	319, 779, 940, 973
AcuI	CTGAAG	1 cut(s)	115
AcyI	GRCGYC	2 cut(s)	272, 395
AfaI	GTAC	2 cut(s)	1218, 1422
AfiI	CCNNNNNNNGG	8 cut(s)	38, 147, 167, 440, 923, 992, 1158, 1496
AgsI	TTSAA	7 cut(s)	358, 520, 540, 839, 851, 979, 1011
AhdI	GACNNNNNGTC	1 cut(s)	238
AjnI	CCWGG	2 cut(s)	171, 1489
AloI	GAACNNNNNNTCC	2 cut(s)	338, 370
AluBI	AGCT	7 cut(s)	135, 253, 307, 953, 1178, 1311, 1427
AluI	AGCT	7 cut(s)	135, 253, 307, 953, 1178, 1311, 1427
Alw21I	GWGCWC	1 cut(s)	806
Alw26I	GTCTC	2 cut(s)	208, 279
Alw44I	GTGCAC	1 cut(s)	802
AlwI	GGATC	3 cut(s)	684, 1367, 1524
Ama87I	CYCGRG	1 cut(s)	308
Aor13HI	TCCGGA	1 cut(s)	38
AoxI	GGCC	2 cut(s)	158, 169
ApaI	GGGCCC	1 cut(s)	162
ApaLI	GTGCAC	1 cut(s)	802
ApoI	RAATTY	4 cut(s)	319, 779, 940, 973
AspLEI	GCGC	2 cut(s)	111, 292
AspS9I	GGNCC	9 cut(s)	41, 158, 159, 169, 188, 388, 443, 1001, 1531
AsuC2I	CCSGG	5 cut(s)	162, 186, 403, 420, 435
AsuHPI	GGTGA	2 cut(s)	423, 778
AsuII	TTCGAA	1 cut(s)	120
AvaI	CYCGRG	1 cut(s)	308
AvaII	GGWCC	6 cut(s)	41, 188, 388, 443, 1001, 1531
AxyI	CCTNAGG	1 cut(s)	1377
BaeGI	GKGCMC	2 cut(s)	162, 806
BanII	GRGCYC	1 cut(s)	162
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	1 cut(s)	806
BccI	CCATC	8 cut(s)	52, 329, 517, 592, 725, 816, 1244, 1507
BceAI	ACGGC	6 cut(s)	19, 238, 346, 382, 501, 629
BcgI	CGANNNNNNTGC	2 cut(s)	100, 134
BciT130I	CCWGG	2 cut(s)	173, 1491
BcnI	CCSGG	5 cut(s)	162, 186, 403, 420, 435
BcoDI	GTCTC	2 cut(s)	208, 279
BfaI	CTAG	2 cut(s)	473, 1206
BfmI	CTRYAG	1 cut(s)	1542
BfoI	RGCGCY	1 cut(s)	293
BfuAI	ACCTGC	1 cut(s)	754
Bme1390I	CCNGG	7 cut(s)	162, 173, 186, 403, 420, 435, 1491
Bme18I	GGWCC	6 cut(s)	41, 188, 388, 443, 1001, 1531
BmeRI	GACNNNNNGTC	1 cut(s)	238
BmeT110I	CYCGRG	1 cut(s)	308
BmgT120I	GGNCC	9 cut(s)	41, 158, 159, 169, 188, 388, 443, 1001, 1531
BmiI	GGNNCC	8 cut(s)	160, 190, 265, 299, 390, 444, 445, 1532
BmrFI	CCNGG	7 cut(s)	162, 173, 186, 403, 420, 435, 1491
BmrI	ACTGGG	1 cut(s)	165
BmsI	GCATC	2 cut(s)	1081, 1475
BmuI	ACTGGG	1 cut(s)	165
BoxI	GACNNNNGTC	1 cut(s)	200
BpiI	GAAGAC	1 cut(s)	693
BplI	GAGNNNNNCTC	2 cut(s)	1349, 1381
Bpu10I	CCTNAGC	1 cut(s)	489
Bpu14I	TTCGAA	1 cut(s)	120
BpuMI	CCSGG	5 cut(s)	162, 186, 403, 420, 435
Bsa29I	ATCGAT	1 cut(s)	1469
BsaAI	YACGTR	1 cut(s)	1354
BsaBI	GATNNNNATC	1 cut(s)	1122
BsaHI	GRCGYC	2 cut(s)	272, 395
BsaJI	CCNNGG	5 cut(s)	172, 184, 284, 1152, 1304
BsaWI	WCCGGW	2 cut(s)	38, 351
BsaXI	ACNNNNNCTCC	4 cut(s)	200, 230, 565, 595
Bsc4I	CCNNNNNNNGG	8 cut(s)	38, 147, 167, 440, 923, 992, 1158, 1496
Bse118I	RCCGGY	1 cut(s)	140
Bse1I	ACTGG	3 cut(s)	160, 821, 1227
Bse21I	CCTNAGG	1 cut(s)	1377
Bse8I	GATNNNNATC	1 cut(s)	1122
BseAI	TCCGGA	1 cut(s)	38
BseBI	CCWGG	2 cut(s)	173, 1491
BseCI	ATCGAT	1 cut(s)	1469
BseDI	CCNNGG	5 cut(s)	172, 184, 284, 1152, 1304
BseGI	GGATG	4 cut(s)	76, 509, 584, 808
BseJI	GATNNNNATC	1 cut(s)	1122
BseLI	CCNNNNNNNGG	8 cut(s)	38, 147, 167, 440, 923, 992, 1158, 1496
BseMII	CTCAG	2 cut(s)	503, 522
BseNI	ACTGG	3 cut(s)	160, 821, 1227
BseRI	GAGGAG	1 cut(s)	326
BseSI	GKGCMC	2 cut(s)	162, 806
BseYI	CCCAGC	2 cut(s)	249, 292
BsgI	GTGCAG	1 cut(s)	483
BshFI	GGCC	2 cut(s)	160, 171
BshVI	ATCGAT	1 cut(s)	1469
BsiHKAI	GWGCWC	1 cut(s)	806
BsiHKCI	CYCGRG	1 cut(s)	308
BslFI	GGGAC	6 cut(s)	374, 456, 900, 1009, 1078, 1544
BslI	CCNNNNNNNGG	8 cut(s)	38, 147, 167, 440, 923, 992, 1158, 1496
BsmAI	GTCTC	2 cut(s)	208, 279
BsmBI	CGTCTC	2 cut(s)	208, 279
BsmFI	GGGAC	6 cut(s)	374, 456, 900, 1009, 1078, 1544
BsmI	GAATGC	1 cut(s)	535
BsnI	GGCC	2 cut(s)	160, 171
BsoBI	CYCGRG	1 cut(s)	308
Bsp119I	TTCGAA	1 cut(s)	120
Bsp120I	GGGCCC	1 cut(s)	158
Bsp1286I	GDGCHC	2 cut(s)	162, 806
Bsp13I	TCCGGA	1 cut(s)	38
Bsp143I	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
BspANI	GGCC	2 cut(s)	160, 171
BspCNI	CTCAG	2 cut(s)	502, 523
BspDI	ATCGAT	1 cut(s)	1469
BspEI	TCCGGA	1 cut(s)	38
BspHI	TCATGA	2 cut(s)	892, 1414
BspLI	GGNNCC	8 cut(s)	160, 190, 265, 299, 390, 444, 445, 1532
BspMI	ACCTGC	1 cut(s)	754
BspPI	GGATC	3 cut(s)	684, 1367, 1524
BspT104I	TTCGAA	1 cut(s)	120
BsrFI	RCCGGY	1 cut(s)	140
BsrI	ACTGG	3 cut(s)	160, 821, 1227
BssAI	RCCGGY	1 cut(s)	140
BssECI	CCNNGG	5 cut(s)	172, 184, 284, 1152, 1304
BssMI	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
BssNI	GRCGYC	2 cut(s)	272, 395
Bst2UI	CCWGG	2 cut(s)	173, 1491
Bst4CI	ACNGT	7 cut(s)	54, 568, 624, 699, 712, 1076, 1195
BstACI	GRCGYC	2 cut(s)	272, 395
BstBAI	YACGTR	1 cut(s)	1354
BstBI	TTCGAA	1 cut(s)	120
BstC8I	GCNNGC	6 cut(s)	63, 142, 258, 468, 884, 1406
BstDEI	CTNAG	7 cut(s)	489, 531, 949, 1017, 1243, 1377, 1520
BstDSI	CCRYGG	1 cut(s)	1304
BstF5I	GGATG	4 cut(s)	76, 509, 584, 808
BstH2I	RGCGCY	1 cut(s)	293
BstHHI	GCGC	2 cut(s)	111, 292
BstKTI	GATC	7 cut(s)	96, 685, 692, 1126, 1375, 1481, 1519
BstMAI	GTCTC	2 cut(s)	208, 279
BstMBI	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
BstMWI	GCNNNNNNNGC	7 cut(s)	31, 141, 234, 289, 313, 494, 1175
BstNI	CCWGG	2 cut(s)	173, 1491
BstNSI	RCATGY	1 cut(s)	1338
BstPAI	GACNNNNGTC	1 cut(s)	200
BstSCI	CCNGG	7 cut(s)	160, 171, 184, 401, 418, 433, 1489
BstSFI	CTRYAG	1 cut(s)	1542
BstSLI	GKGCMC	2 cut(s)	162, 806
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	3 cut(s)	689, 1372, 1516
BstXI	CCANNNNNNTGG	1 cut(s)	965
BstYI	RGATCY	3 cut(s)	689, 1372, 1516
Bsu15I	ATCGAT	1 cut(s)	1469
Bsu36I	CCTNAGG	1 cut(s)	1377
BsuRI	GGCC	2 cut(s)	160, 171
BsuTUI	ATCGAT	1 cut(s)	1469
BtgI	CCRYGG	1 cut(s)	1304
BtgZI	GCGATG	2 cut(s)	30, 348
BtsCI	GGATG	4 cut(s)	76, 509, 584, 808
BtsIMutI	CAGTG	3 cut(s)	573, 1081, 1234
BveI	ACCTGC	1 cut(s)	754
Cac8I	GCNNGC	6 cut(s)	63, 142, 258, 468, 884, 1406
CciI	TCATGA	2 cut(s)	892, 1414
CfoI	GCGC	2 cut(s)	111, 292
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	9 cut(s)	41, 158, 159, 169, 188, 388, 443, 1001, 1531
ClaI	ATCGAT	1 cut(s)	1469
CseI	GACGC	3 cut(s)	403, 902, 1013
Csp6I	GTAC	2 cut(s)	1217, 1421
CviAII	CATG	7 cut(s)	193, 268, 893, 965, 1049, 1335, 1415
CviQI	GTAC	2 cut(s)	1217, 1421
DdeI	CTNAG	7 cut(s)	489, 531, 949, 1017, 1243, 1377, 1520
DpnI	GATC	7 cut(s)	95, 684, 691, 1125, 1374, 1480, 1518
DpnII	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
DraI	TTTAAA	1 cut(s)	1560
DrdI	GACNNNNNNGTC	1 cut(s)	277
DriI	GACNNNNNGTC	1 cut(s)	238
DseDI	GACNNNNNNGTC	1 cut(s)	277
Eam1105I	GACNNNNNGTC	1 cut(s)	238
EciI	GGCGGA	2 cut(s)	127, 760
Eco24I	GRGCYC	1 cut(s)	162
Eco47I	GGWCC	6 cut(s)	41, 188, 388, 443, 1001, 1531
Eco57I	CTGAAG	1 cut(s)	115
Eco81I	CCTNAGG	1 cut(s)	1377
Eco88I	CYCGRG	1 cut(s)	308
EcoO109I	RGGNCCY	2 cut(s)	443, 1001
EcoRII	CCWGG	2 cut(s)	171, 1489
EcoT22I	ATGCAT	1 cut(s)	1468
EcoT38I	GRGCYC	1 cut(s)	162
Esp3I	CGTCTC	2 cut(s)	208, 279
FaeI	CATG	7 cut(s)	196, 271, 896, 968, 1052, 1338, 1418
FaqI	GGGAC	6 cut(s)	374, 456, 900, 1009, 1078, 1544
FatI	CATG	7 cut(s)	192, 267, 892, 964, 1048, 1334, 1414
FauI	CCCGC	6 cut(s)	70, 92, 111, 140, 309, 568
FauNDI	CATATG	1 cut(s)	1396
FblI	GTMKAC	1 cut(s)	1236
FokI	GGATG	4 cut(s)	63, 496, 571, 795
FriOI	GRGCYC	1 cut(s)	162
FspBI	CTAG	2 cut(s)	473, 1206
GlaI	GCGC	2 cut(s)	110, 291
GsaI	CCCAGC	2 cut(s)	253, 296
HaeII	RGCGCY	1 cut(s)	293
HaeIII	GGCC	2 cut(s)	160, 171
HgaI	GACGC	3 cut(s)	403, 902, 1013
HhaI	GCGC	2 cut(s)	111, 292
Hin1I	GRCGYC	2 cut(s)	272, 395
Hin1II	CATG	7 cut(s)	196, 271, 896, 968, 1052, 1338, 1418
Hin6I	GCGC	2 cut(s)	109, 290
HinP1I	GCGC	2 cut(s)	109, 290
HincII	GTYRAC	3 cut(s)	573, 1237, 1507
HindII	GTYRAC	3 cut(s)	573, 1237, 1507
HinfI	GANTC	9 cut(s)	70, 122, 196, 413, 423, 659, 1031, 1209, 1486
HpaI	GTTAAC	1 cut(s)	1507
HphI	GGTGA	2 cut(s)	423, 778
Hpy166II	GTNNAC	5 cut(s)	573, 804, 1237, 1507, 1553
Hpy188I	TCNGA	9 cut(s)	220, 346, 598, 667, 682, 687, 946, 1165, 1191
Hpy188III	TCNNGA	7 cut(s)	39, 391, 410, 627, 893, 1028, 1415
Hpy8I	GTNNAC	5 cut(s)	573, 804, 1237, 1507, 1553
Hpy99I	CGWCG	6 cut(s)	58, 224, 233, 283, 332, 397
HpyAV	CCTTC	5 cut(s)	253, 826, 845, 986, 1495
HpyCH4III	ACNGT	7 cut(s)	54, 568, 624, 699, 712, 1076, 1195
HpyCH4IV	ACGT	6 cut(s)	272, 898, 1325, 1341, 1353, 1555
HpyCH4V	TGCA	6 cut(s)	237, 500, 804, 1323, 1334, 1466
HpyF10VI	GCNNNNNNNGC	7 cut(s)	31, 141, 234, 289, 313, 494, 1175
HpyF3I	CTNAG	7 cut(s)	489, 531, 949, 1017, 1243, 1377, 1520
HpySE526I	ACGT	6 cut(s)	272, 898, 1325, 1341, 1353, 1555
Hsp92I	GRCGYC	2 cut(s)	272, 395
Hsp92II	CATG	7 cut(s)	196, 271, 896, 968, 1052, 1338, 1418
HspAI	GCGC	2 cut(s)	109, 290
KflI	GGGWCCC	1 cut(s)	443
Kpn2I	TCCGGA	1 cut(s)	38
KroI	GCCGGC	1 cut(s)	140
KroNI	GCCGGC	1 cut(s)	142
KspAI	GTTAAC	1 cut(s)	1507
Kzo9I	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
LmnI	GCTCC	4 cut(s)	140, 263, 313, 1308
LweI	GCATC	2 cut(s)	1081, 1475
MaeI	CTAG	2 cut(s)	473, 1206
MaeII	ACGT	6 cut(s)	272, 898, 1325, 1341, 1353, 1555
MaeIII	GTNAC	6 cut(s)	481, 734, 1070, 1195, 1253, 1337
MalI	GATC	7 cut(s)	95, 684, 691, 1125, 1374, 1480, 1518
MboI	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
MboII	GAAGA	8 cut(s)	121, 205, 418, 693, 841, 1100, 1133, 1155
MfeI	CAATTG	1 cut(s)	739
MflI	RGATCY	3 cut(s)	689, 1372, 1516
MhlI	GDGCHC	2 cut(s)	162, 806
MluCI	AATT	9 cut(s)	319, 520, 739, 779, 814, 867, 940, 973, 1054
MlyI	GAGTC	3 cut(s)	64, 190, 407
MmeI	TCCRAC	3 cut(s)	231, 243, 969
Mph1103I	ATGCAT	1 cut(s)	1468
MreI	CGCCGGCG	1 cut(s)	140
MroI	TCCGGA	1 cut(s)	38
MroNI	GCCGGC	1 cut(s)	140
MseI	TTAA	3 cut(s)	1475, 1506, 1559
MslI	CAYNNNNRTG	2 cut(s)	891, 1413
MspA1I	CMGCKG	2 cut(s)	253, 1172
MspR9I	CCNGG	7 cut(s)	162, 173, 186, 403, 420, 435, 1491
MunI	CAATTG	1 cut(s)	739
Mva1269I	GAATGC	1 cut(s)	535
MvaI	CCWGG	2 cut(s)	173, 1491
MwoI	GCNNNNNNNGC	7 cut(s)	31, 141, 234, 289, 313, 494, 1175
NaeI	GCCGGC	1 cut(s)	142
NciI	CCSGG	5 cut(s)	162, 186, 403, 420, 435
NdeI	CATATG	1 cut(s)	1396
NdeII	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
NgoMIV	GCCGGC	1 cut(s)	140
NlaIII	CATG	7 cut(s)	196, 271, 896, 968, 1052, 1338, 1418
NlaIV	GGNNCC	8 cut(s)	160, 190, 265, 299, 390, 444, 445, 1532
NmeAIII	GCCGAG	1 cut(s)	309
NmuCI	GTSAC	2 cut(s)	481, 1070
NsiI	ATGCAT	1 cut(s)	1468
NspI	RCATGY	1 cut(s)	1338
NspV	TTCGAA	1 cut(s)	120
PaeR7I	CTCGAG	1 cut(s)	308
PagI	TCATGA	2 cut(s)	892, 1414
PaqCI	CACCTGC	1 cut(s)	754
PcsI	WCGNNNNNNNCGW	2 cut(s)	53, 228
PctI	GAATGC	1 cut(s)	535
PdiI	GCCGGC	1 cut(s)	142
PfeI	GAWTC	6 cut(s)	122, 423, 659, 1031, 1209, 1486
PflFI	GACNNNGTC	1 cut(s)	397
PflMI	CCANNNNNTGG	1 cut(s)	1496
PfoI	TCCNGGA	1 cut(s)	1489
PleI	GAGTC	3 cut(s)	64, 190, 407
PpsI	GAGTC	3 cut(s)	64, 190, 407
Ppu21I	YACGTR	1 cut(s)	1354
PpuMI	RGGWCCY	2 cut(s)	443, 1001
PshAI	GACNNNNGTC	1 cut(s)	200
Psp5II	RGGWCCY	2 cut(s)	443, 1001
Psp6I	CCWGG	2 cut(s)	171, 1489
PspFI	CCCAGC	2 cut(s)	249, 292
PspGI	CCWGG	2 cut(s)	171, 1489
PspN4I	GGNNCC	8 cut(s)	160, 190, 265, 299, 390, 444, 445, 1532
PspOMI	GGGCCC	1 cut(s)	158
PspPI	GGNCC	9 cut(s)	41, 158, 159, 169, 188, 388, 443, 1001, 1531
PspPPI	RGGWCCY	2 cut(s)	443, 1001
PspXI	VCTCGAGB	1 cut(s)	308
PsuI	RGATCY	3 cut(s)	689, 1372, 1516
PsyI	GACNNNGTC	1 cut(s)	397
PvuII	CAGCTG	1 cut(s)	253
RsaI	GTAC	2 cut(s)	1218, 1422
RsaNI	GTAC	2 cut(s)	1217, 1421
RseI	CAYNNNNRTG	2 cut(s)	891, 1413
SalI	GTCGAC	1 cut(s)	1235
SaqAI	TTAA	3 cut(s)	1475, 1506, 1559
Sau3AI	GATC	7 cut(s)	93, 682, 689, 1123, 1372, 1478, 1516
Sau96I	GGNCC	9 cut(s)	41, 158, 159, 169, 188, 388, 443, 1001, 1531
SchI	GAGTC	3 cut(s)	64, 190, 407
ScrFI	CCNGG	7 cut(s)	162, 173, 186, 403, 420, 435, 1491
SduI	GDGCHC	2 cut(s)	162, 806
SfaNI	GCATC	2 cut(s)	1081, 1475
SfcI	CTRYAG	1 cut(s)	1542
Sfr274I	CTCGAG	1 cut(s)	308
SfuI	TTCGAA	1 cut(s)	120
SgrAI	CRCCGGYG	1 cut(s)	140
SinI	GGWCC	6 cut(s)	41, 188, 388, 443, 1001, 1531
SlaI	CTCGAG	1 cut(s)	308
SmiMI	CAYNNNNRTG	2 cut(s)	891, 1413
SmlI	CTYRAG	1 cut(s)	308
SmoI	CTYRAG	1 cut(s)	308
Sse9I	AATT	9 cut(s)	319, 520, 739, 779, 814, 867, 940, 973, 1054
SspMI	CTAG	2 cut(s)	473, 1206
StyD4I	CCNGG	7 cut(s)	160, 171, 184, 401, 418, 433, 1489
TaaI	ACNGT	7 cut(s)	54, 568, 624, 699, 712, 1076, 1195
TaiI	ACGT	6 cut(s)	275, 901, 1328, 1344, 1356, 1558
TaqI	TCGA	9 cut(s)	56, 120, 309, 411, 714, 787, 845, 1236, 1469
TaqII	GACCGA	1 cut(s)	376
TasI	AATT	9 cut(s)	319, 520, 739, 779, 814, 867, 940, 973, 1054
TatI	WGTACW	1 cut(s)	1216
TauI	GCSGC	5 cut(s)	34, 147, 228, 376, 1175
TfiI	GAWTC	6 cut(s)	122, 423, 659, 1031, 1209, 1486
Tru1I	TTAA	3 cut(s)	1475, 1506, 1559
Tru9I	TTAA	3 cut(s)	1475, 1506, 1559
TscAI	CASTG	3 cut(s)	573, 1081, 1234
TseFI	GTSAC	2 cut(s)	481, 1070
Tsp45I	GTSAC	2 cut(s)	481, 1070
TspDTI	ATGAA	4 cut(s)	693, 909, 953, 1134
TspGWI	ACGGA	3 cut(s)	267, 1321, 1334
TspRI	CASTG	3 cut(s)	573, 1081, 1234
Tth111I	GACNNNGTC	1 cut(s)	397
Van91I	CCANNNNNTGG	1 cut(s)	1496
VneI	GTGCAC	1 cut(s)	802
VpaK11BI	GGWCC	6 cut(s)	41, 188, 388, 443, 1001, 1531
XapI	RAATTY	4 cut(s)	319, 779, 940, 973
XceI	RCATGY	1 cut(s)	1338
XcmI	CCANNNNNNNNNTGG	1 cut(s)	965
XhoI	CTCGAG	1 cut(s)	308
XmiI	GTMKAC	1 cut(s)	1236
XspI	CTAG	2 cut(s)	473, 1206
ZraI	GACGTC	1 cut(s)	273
Zsp2I	ATGCAT	1 cut(s)	1468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.