Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007326

Physical Location & Seq

Genomic Coordinates Forward (+)

4642014 .. 4644789

Gene Structure 2776 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1066500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCCGACAACCAAGGGCAACCGCCGGGCCCCCTGCCGCCAGGATCATCGCAACCGTCGAAGCCCGCAGCGACGCATGATCCTCCTCGCCCCACGATTACTCTCCCGCCGCGCACCTCTTTCGAATCAATCTTCAGCTCCGGCGCCGGCGGGAATACTGGCCCGGGTCTGGGCATGGGCTTCAGCCCCGGGCCCATGACTCTCGTCTCCAACTTCTTCTCCGACGGTGACGACTGCAAGTCCTTCTCCCAGCTGCTCGCCGGAGCCATGACGTCTCCATCCCCGAGGGAGCCGGGGTTCCCGCAGCTAGAAAAGCGAAATTCCGGCGATAACGATGGCGGCTCGGAATTCCGGTTCAAGCAGAACAGAGCGCCGCCGATGTTTTCGGTCCCGACGCCGTCCGGGTTTCTCGACTCGCCGGGTATCTTCTCACCCGGCCAGGGACCCTTTGGGATGACACACCAGCAGGCGCTAGCACAGGTCACGGCTCAGGCTGCTGCACTCTCACCATCCTTTTTCAATTTCTCAACCGAGTTTTCAACGACTTTCTCGACAGCACCCGCCACATCGTTGACGCAGCCTCCATCCCTTACTTCCGATACAATGCCACCCCAAGAGATACCGTCGGGAACACCCGACTCTGCCGTTGCCACTAAAGAATCATCCGACATTGCCCTTTCGGATCAGAGATCCCAACCGTCTTCATTTGCCGTCGATAAGCCTAATGACGATGGTTACAATTGGCGGAAGTATGGGCAAAAGCAGGTCAAGGGTAGTGAGTTTCCTCGGAGTTATTACAAGTGCACGCATCCAAAGTGTCCGGTAAAGAAGAAGGTTGAACGCTCGGTTGATGGACAAATAACTGAAATTATTTACAAGGGCGAGCATAACCATGAACGTCCTCCGTCCAAGCGTGGAAAGGATCCGAATGCAAATAGTCAGGCGAATTCCGACTTGGCCTTGCAAGTTCATGGTGGGAATTCGAACAAATCAAAAGACGGGACAGGTCCTTATTCAATGTCTAAGAAGCGTCAGGAATCCAGCCAAGCAACACATGATAATTTATCCGGGACAAGTGACAGCGAGGAAGTAGGAGATGCTGAAACCAGAGTAGGTGAGAAAGATGAAGATCAACCTGACACCAAGAGAAGAAATACAGAGGTCAGGTCAGAACTGGCGCCAGCTTCTGCACATCGGACTGTGACAGAGCCTAGAATCATAGTGCAGACAACCAGTGAAGTCGATCTCTTAGACGATGGCTACAGGTGGCGAAAGTATGGGCAGAAAGTCGTCAAAGGAAATCCCTATCCACGGAGCTATTACAAATGCACATTCCCTGCATGCAATGTCCGTAAGCACATAGAGAGAGCCTCTACAGATCCTAAAGCTGTCATAACGACATATGAGGGAAAACATAATCATGATGTACCAGCTTCTAAAACAAGCGGCCACTACACAGGCAACAACAACGCATCAAATCTAAGATCAGTCAATCCAGGAACTGGGAAGATGGACAAGATCGATCTTAGAAACAAGGACCAGCAGCCTATAGCAAGTTTACGTTTAAAAGAAGAACAAATAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009753 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043901 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0140110 GO:1900150 GO:1900424 GO:1900425 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

529

Amino Acids

57.2

Weight (kDa)

8.38

Isoelectric Point (pI)

55.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	243 - 299	9.4e-28	WRKY DNA -binding domain
WRKY	PF03106	418 - 475	4.2e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13960 AT1G13960 AT2G03340
fragaria_vesca	FvH4_4g30640 FvH4_4g30640 FvH4_4g30640 FvH4_4g30640
malus_domestica	MD13G1067600.v1.1 MD16G1066500.v1.1
prunus_persica	Prupe.1G280700_v2.0.a1 Prupe.1G280700_v2.0.a1
pyrus_communis	pycom13g05940 pycom16g05790
rosa_chinensis	RchiOBHm_Chr4g0439041
rosa_laevigata	RLG00000006307
rosa_multiflora	Rmu_sc0001338.1_g000004 Rmu_sc0003226.1_g000090
rosa_roxburghii	Rroxscaffold_5G00380040
rosa_rugosa	Rorug04G0315200
rosa_samantha	Rh4AG365900 Rh4BG377200 Rh4CG391300 Rh4DG371900
rosa_wichuraiana	Rw0G007420 Rw0G016970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	275
Acc36I	ACCTGC	1 cut(s)	754
AccB1I	GGYRCC	2 cut(s)	143, 1177
AccB7I	CCANNNNNTGG	1 cut(s)	1502
AccII	CGCG	1 cut(s)	112
AclWI	GGATC	7 cut(s)	52, 74, 684, 690, 917, 930, 1373
AcoI	YGGCCR	3 cut(s)	3, 436, 1447
AcsI	RAATTY	4 cut(s)	319, 347, 946, 979
AcuI	CTGAAG	2 cut(s)	118, 166
AcyI	GRCGYC	4 cut(s)	144, 272, 395, 1178
AfaI	GTAC	1 cut(s)	1428
AfiI	CCNNNNNNNGG	5 cut(s)	169, 170, 440, 1311, 1502
AgsI	TTSAA	5 cut(s)	358, 520, 540, 839, 1017
AhdI	GACNNNNNGTC	1 cut(s)	238
AjnI	CCWGG	3 cut(s)	40, 438, 1495
AjuI	GAANNNNNNNTTGG	2 cut(s)	938, 970
AloI	GAACNNNNNNTCC	4 cut(s)	281, 313, 338, 370
AluBI	AGCT	7 cut(s)	138, 253, 307, 1184, 1317, 1388, 1433
AluI	AGCT	7 cut(s)	138, 253, 307, 1184, 1317, 1388, 1433
Alw21I	GWGCWC	1 cut(s)	806
Alw26I	GTCTC	2 cut(s)	211, 279
Alw44I	GTGCAC	1 cut(s)	802
AlwI	GGATC	7 cut(s)	52, 74, 684, 690, 917, 930, 1373
AlwNI	CAGNNNCTG	2 cut(s)	1187, 1502
Ama87I	CYCGRG	3 cut(s)	163, 188, 283
AoxI	GGCC	7 cut(s)	3, 28, 160, 191, 436, 957, 1447
ApaI	GGGCCC	2 cut(s)	32, 195
ApaLI	GTGCAC	1 cut(s)	802
ApeKI	GCWGC	7 cut(s)	68, 253, 304, 494, 497, 577, 1543
ApoI	RAATTY	4 cut(s)	319, 347, 946, 979
AspLEI	GCGC	5 cut(s)	114, 146, 373, 472, 1180
AspS9I	GGNCC	9 cut(s)	28, 29, 161, 191, 192, 388, 443, 1007, 1537
AsuHPI	GGTGA	4 cut(s)	239, 423, 498, 1127
AsuII	TTCGAA	2 cut(s)	123, 983
AsuNHI	GCTAGC	1 cut(s)	472
AvaI	CYCGRG	3 cut(s)	163, 188, 283
AvaII	GGWCC	4 cut(s)	388, 443, 1007, 1537
BaeGI	GKGCMC	3 cut(s)	32, 195, 806
BamHI	GGATCC	1 cut(s)	922
BanI	GGYRCC	2 cut(s)	143, 1177
BanII	GRGCYC	2 cut(s)	32, 195
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	1 cut(s)	806
BbvI	GCAGC	7 cut(s)	80, 240, 316, 481, 484, 589, 1555
BccI	CCATC	8 cut(s)	286, 329, 517, 592, 725, 845, 1250, 1504
BceAI	ACGGC	4 cut(s)	382, 501, 629, 695
BcgI	CGANNNNNNTGC	2 cut(s)	103, 137
BciT130I	CCWGG	3 cut(s)	42, 440, 1497
BcoDI	GTCTC	2 cut(s)	211, 279
BfaI	CTAG	3 cut(s)	308, 473, 1212
BfmI	CTRYAG	3 cut(s)	1261, 1374, 1548
BfoI	RGCGCY	4 cut(s)	147, 374, 473, 1181
BfuAI	ACCTGC	1 cut(s)	754
Bme18I	GGWCC	4 cut(s)	388, 443, 1007, 1537
BmeRI	GACNNNNNGTC	1 cut(s)	238
BmeT110I	CYCGRG	3 cut(s)	163, 188, 283
BmgT120I	GGNCC	9 cut(s)	28, 29, 161, 191, 192, 388, 443, 1007, 1537
BmrI	ACTGGG	1 cut(s)	1512
BmsI	GCATC	3 cut(s)	817, 1087, 1481
BmtI	GCTAGC	1 cut(s)	476
BmuI	ACTGGG	1 cut(s)	1512
BoxI	GACNNNNGTC	1 cut(s)	203
BpiI	GAAGAC	1 cut(s)	693
BplI	GAGNNNNNCTC	2 cut(s)	1355, 1387
Bpu10I	CCTNAGC	1 cut(s)	489
Bpu14I	TTCGAA	2 cut(s)	123, 983
Bsa29I	ATCGAT	1 cut(s)	1521
BsaBI	GATNNNNATC	1 cut(s)	1128
BsaHI	GRCGYC	4 cut(s)	144, 272, 395, 1178
BsaJI	CCNNGG	9 cut(s)	13, 163, 187, 188, 284, 293, 439, 785, 1310
BsaWI	WCCGGW	2 cut(s)	351, 820
BsaXI	ACNNNNNCTCC	6 cut(s)	203, 233, 281, 311, 565, 595
Bsc4I	CCNNNNNNNGG	5 cut(s)	169, 170, 440, 1311, 1502
Bse118I	RCCGGY	1 cut(s)	146
Bse1I	ACTGG	4 cut(s)	163, 1179, 1233, 1507
Bse3DI	GCAATG	2 cut(s)	669, 1351
Bse8I	GATNNNNATC	1 cut(s)	1128
BseBI	CCWGG	3 cut(s)	42, 440, 1497
BseCI	ATCGAT	1 cut(s)	1521
BseDI	CCNNGG	9 cut(s)	13, 163, 187, 188, 284, 293, 439, 785, 1310
BseGI	GGATG	6 cut(s)	278, 459, 509, 584, 662, 808
BseJI	GATNNNNATC	1 cut(s)	1128
BseLI	CCNNNNNNNGG	5 cut(s)	169, 170, 440, 1311, 1502
BseMI	GCAATG	2 cut(s)	669, 1351
BseMII	CTCAG	1 cut(s)	503
BseNI	ACTGG	4 cut(s)	163, 1179, 1233, 1507
BseRI	GAGGAG	1 cut(s)	75
BseSI	GKGCMC	3 cut(s)	32, 195, 806
BseXI	GCAGC	7 cut(s)	80, 240, 316, 481, 484, 589, 1555
BseYI	CCCAGC	1 cut(s)	249
BsgI	GTGCAG	3 cut(s)	483, 1173, 1244
Bsh1236I	CGCG	1 cut(s)	112
BshFI	GGCC	7 cut(s)	5, 30, 162, 193, 438, 959, 1449
BshNI	GGYRCC	2 cut(s)	143, 1177
BshVI	ATCGAT	1 cut(s)	1521
BsiHKAI	GWGCWC	1 cut(s)	806
BsiHKCI	CYCGRG	3 cut(s)	163, 188, 283
BslFI	GGGAC	4 cut(s)	374, 456, 1015, 1084
BslI	CCNNNNNNNGG	5 cut(s)	169, 170, 440, 1311, 1502
BsmAI	GTCTC	2 cut(s)	211, 279
BsmBI	CGTCTC	2 cut(s)	211, 279
BsmFI	GGGAC	4 cut(s)	374, 456, 1015, 1084
BsmI	GAATGC	1 cut(s)	934
BsnI	GGCC	7 cut(s)	5, 30, 162, 193, 438, 959, 1449
BsoBI	CYCGRG	3 cut(s)	163, 188, 283
Bsp119I	TTCGAA	2 cut(s)	123, 983
Bsp120I	GGGCCC	2 cut(s)	28, 191
Bsp1286I	GDGCHC	3 cut(s)	32, 195, 806
BspANI	GGCC	7 cut(s)	5, 30, 162, 193, 438, 959, 1449
BspCNI	CTCAG	1 cut(s)	502
BspDI	ATCGAT	1 cut(s)	1521
BspFNI	CGCG	1 cut(s)	112
BspHI	TCATGA	1 cut(s)	1420
BspMI	ACCTGC	1 cut(s)	754
BspOI	GCTAGC	1 cut(s)	476
BspPI	GGATC	7 cut(s)	52, 74, 684, 690, 917, 930, 1373
BspT104I	TTCGAA	2 cut(s)	123, 983
BspT107I	GGYRCC	2 cut(s)	143, 1177
BsrDI	GCAATG	2 cut(s)	669, 1351
BsrFI	RCCGGY	1 cut(s)	146
BsrI	ACTGG	4 cut(s)	163, 1179, 1233, 1507
BssAI	RCCGGY	1 cut(s)	146
BssECI	CCNNGG	9 cut(s)	13, 163, 187, 188, 284, 293, 439, 785, 1310
BssNI	GRCGYC	4 cut(s)	144, 272, 395, 1178
BssT1I	CCWWGG	1 cut(s)	13
Bst2UI	CCWGG	3 cut(s)	42, 440, 1497
Bst4CI	ACNGT	5 cut(s)	57, 227, 624, 699, 1201
BstACI	GRCGYC	4 cut(s)	144, 272, 395, 1178
BstBI	TTCGAA	2 cut(s)	123, 983
BstC8I	GCNNGC	9 cut(s)	66, 148, 258, 468, 474, 806, 884, 1182, 1342
BstDEI	CTNAG	5 cut(s)	489, 1023, 1249, 1481, 1526
BstDSI	CCRYGG	1 cut(s)	1310
BstF5I	GGATG	6 cut(s)	278, 459, 509, 584, 662, 808
BstFNI	CGCG	1 cut(s)	112
BstH2I	RGCGCY	4 cut(s)	147, 374, 473, 1181
BstHHI	GCGC	5 cut(s)	114, 146, 373, 472, 1180
BstMAI	GTCTC	2 cut(s)	211, 279
BstMWI	GCNNNNNNNGC	2 cut(s)	313, 494
BstNI	CCWGG	3 cut(s)	42, 440, 1497
BstNSI	RCATGY	1 cut(s)	1344
BstPAI	GACNNNNGTC	1 cut(s)	203
BstSFI	CTRYAG	3 cut(s)	1261, 1374, 1548
BstSLI	GKGCMC	3 cut(s)	32, 195, 806
BstUI	CGCG	1 cut(s)	112
BstV1I	GCAGC	7 cut(s)	80, 240, 316, 481, 484, 589, 1555
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	3 cut(s)	689, 922, 1378
BstYI	RGATCY	3 cut(s)	689, 922, 1378
Bsu15I	ATCGAT	1 cut(s)	1521
BsuRI	GGCC	7 cut(s)	5, 30, 162, 193, 438, 959, 1449
BsuTUI	ATCGAT	1 cut(s)	1521
BtgI	CCRYGG	1 cut(s)	1310
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	6 cut(s)	278, 459, 509, 584, 662, 808
BtsIMutI	CAGTG	1 cut(s)	1240
BveI	ACCTGC	1 cut(s)	754
Cac8I	GCNNGC	9 cut(s)	66, 148, 258, 468, 474, 806, 884, 1182, 1342
CaiI	CAGNNNCTG	2 cut(s)	1187, 1502
CciI	TCATGA	1 cut(s)	1420
CfoI	GCGC	5 cut(s)	114, 146, 373, 472, 1180
Cfr10I	RCCGGY	1 cut(s)	146
Cfr13I	GGNCC	9 cut(s)	28, 29, 161, 191, 192, 388, 443, 1007, 1537
Cfr9I	CCCGGG	2 cut(s)	163, 188
ClaI	ATCGAT	1 cut(s)	1521
CseI	GACGC	4 cut(s)	82, 403, 583, 1019
Csp6I	GTAC	1 cut(s)	1427
CviAII	CATG	9 cut(s)	77, 175, 196, 268, 893, 971, 1055, 1341, 1421
CviQI	GTAC	1 cut(s)	1427
DdeI	CTNAG	5 cut(s)	489, 1023, 1249, 1481, 1526
DinI	GGCGCC	2 cut(s)	145, 1179
DraI	TTTAAA	1 cut(s)	1566
DriI	GACNNNNNGTC	1 cut(s)	238
EaeI	YGGCCR	3 cut(s)	3, 436, 1447
Eam1105I	GACNNNNNGTC	1 cut(s)	238
EciI	GGCGGA	1 cut(s)	760
Eco130I	CCWWGG	1 cut(s)	13
Eco24I	GRGCYC	2 cut(s)	32, 195
Eco47I	GGWCC	4 cut(s)	388, 443, 1007, 1537
Eco57I	CTGAAG	2 cut(s)	118, 166
Eco88I	CYCGRG	3 cut(s)	163, 188, 283
EcoO109I	RGGNCCY	3 cut(s)	29, 443, 1007
EcoRI	GAATTC	3 cut(s)	347, 946, 979
EcoRII	CCWGG	3 cut(s)	40, 438, 1495
EcoT14I	CCWWGG	1 cut(s)	13
EcoT38I	GRGCYC	2 cut(s)	32, 195
EgeI	GGCGCC	2 cut(s)	145, 1179
EheI	GGCGCC	2 cut(s)	145, 1179
ErhI	CCWWGG	1 cut(s)	13
Esp3I	CGTCTC	2 cut(s)	211, 279
FaeI	CATG	9 cut(s)	80, 178, 199, 271, 896, 974, 1058, 1344, 1424
FaqI	GGGAC	4 cut(s)	374, 456, 1015, 1084
FatI	CATG	9 cut(s)	76, 174, 195, 267, 892, 970, 1054, 1340, 1420
FauI	CCCGC	5 cut(s)	73, 114, 143, 309, 568
FauNDI	CATATG	1 cut(s)	1402
FokI	GGATG	6 cut(s)	265, 466, 496, 571, 649, 795
FriOI	GRGCYC	2 cut(s)	32, 195
FspBI	CTAG	3 cut(s)	308, 473, 1212
GlaI	GCGC	5 cut(s)	113, 145, 372, 471, 1179
GsaI	CCCAGC	1 cut(s)	253
HaeII	RGCGCY	4 cut(s)	147, 374, 473, 1181
HaeIII	GGCC	7 cut(s)	5, 30, 162, 193, 438, 959, 1449
HgaI	GACGC	4 cut(s)	82, 403, 583, 1019
HhaI	GCGC	5 cut(s)	114, 146, 373, 472, 1180
Hin1I	GRCGYC	4 cut(s)	144, 272, 395, 1178
Hin1II	CATG	9 cut(s)	80, 178, 199, 271, 896, 974, 1058, 1344, 1424
Hin6I	GCGC	5 cut(s)	112, 144, 371, 470, 1178
HinP1I	GCGC	5 cut(s)	112, 144, 371, 470, 1178
HincII	GTYRAC	1 cut(s)	573
HindII	GTYRAC	1 cut(s)	573
HinfI	GANTC	7 cut(s)	125, 199, 413, 638, 659, 1037, 1215
HphI	GGTGA	4 cut(s)	239, 423, 498, 1127
Hpy166II	GTNNAC	3 cut(s)	573, 804, 1559
Hpy188III	TCNNGA	6 cut(s)	391, 410, 550, 627, 1034, 1421
Hpy8I	GTNNAC	3 cut(s)	573, 804, 1559
Hpy99I	CGWCG	6 cut(s)	61, 76, 227, 397, 628, 716
HpyAV	CCTTC	2 cut(s)	253, 826
HpyCH4III	ACNGT	5 cut(s)	57, 227, 624, 699, 1201
HpyCH4IV	ACGT	3 cut(s)	272, 898, 1561
HpyF10VI	GCNNNNNNNGC	2 cut(s)	313, 494
HpyF3I	CTNAG	5 cut(s)	489, 1023, 1249, 1481, 1526
HpySE526I	ACGT	3 cut(s)	272, 898, 1561
Hsp92I	GRCGYC	4 cut(s)	144, 272, 395, 1178
Hsp92II	CATG	9 cut(s)	80, 178, 199, 271, 896, 974, 1058, 1344, 1424
HspAI	GCGC	5 cut(s)	112, 144, 371, 470, 1178
KasI	GGCGCC	2 cut(s)	143, 1177
KflI	GGGWCCC	1 cut(s)	443
KroI	GCCGGC	1 cut(s)	146
KroNI	GCCGGC	1 cut(s)	148
LmnI	GCTCC	4 cut(s)	143, 263, 289, 1314
Lsp1109I	GCAGC	7 cut(s)	80, 240, 316, 481, 484, 589, 1555
LweI	GCATC	3 cut(s)	817, 1087, 1481
MaeI	CTAG	3 cut(s)	308, 473, 1212
MaeII	ACGT	3 cut(s)	272, 898, 1561
MaeIII	GTNAC	5 cut(s)	227, 481, 734, 1076, 1201
MboII	GAAGA	9 cut(s)	124, 208, 418, 693, 841, 1139, 1161, 1519, 1583
MfeI	CAATTG	1 cut(s)	739
MflI	RGATCY	3 cut(s)	689, 922, 1378
MhlI	GDGCHC	3 cut(s)	32, 195, 806
MluCI	AATT	8 cut(s)	319, 347, 520, 739, 867, 946, 979, 1060
Mly113I	GGCGCC	2 cut(s)	144, 1178
MlyI	GAGTC	3 cut(s)	193, 407, 632
MmeI	TCCRAC	4 cut(s)	234, 246, 690, 975
MreI	CGCCGGCG	1 cut(s)	146
MroNI	GCCGGC	1 cut(s)	146
MseI	TTAA	1 cut(s)	1565
MslI	CAYNNNNRTG	2 cut(s)	891, 1419
MspA1I	CMGCKG	1 cut(s)	253
MunI	CAATTG	1 cut(s)	739
Mva1269I	GAATGC	1 cut(s)	934
MvaI	CCWGG	3 cut(s)	42, 440, 1497
MvnI	CGCG	1 cut(s)	112
MwoI	GCNNNNNNNGC	2 cut(s)	313, 494
NaeI	GCCGGC	1 cut(s)	148
NarI	GGCGCC	2 cut(s)	144, 1178
NdeI	CATATG	1 cut(s)	1402
NgoMIV	GCCGGC	1 cut(s)	146
NheI	GCTAGC	1 cut(s)	472
NlaIII	CATG	9 cut(s)	80, 178, 199, 271, 896, 974, 1058, 1344, 1424
NmuCI	GTSAC	4 cut(s)	227, 481, 1076, 1201
NspI	RCATGY	1 cut(s)	1344
NspV	TTCGAA	2 cut(s)	123, 983
PaeI	GCATGC	1 cut(s)	1344
PagI	TCATGA	1 cut(s)	1420
PcsI	WCGNNNNNNNCGW	2 cut(s)	56, 548
PctI	GAATGC	1 cut(s)	934
PdiI	GCCGGC	1 cut(s)	148
PfeI	GAWTC	4 cut(s)	125, 659, 1037, 1215
PflFI	GACNNNGTC	1 cut(s)	397
PflMI	CCANNNNNTGG	1 cut(s)	1502
PfoI	TCCNGGA	2 cut(s)	1067, 1495
PleI	GAGTC	3 cut(s)	193, 407, 632
PluTI	GGCGCC	2 cut(s)	147, 1181
PpsI	GAGTC	3 cut(s)	193, 407, 632
PpuMI	RGGWCCY	2 cut(s)	443, 1007
PshAI	GACNNNNGTC	1 cut(s)	203
Psp5II	RGGWCCY	2 cut(s)	443, 1007
Psp6I	CCWGG	3 cut(s)	40, 438, 1495
PspFI	CCCAGC	1 cut(s)	249
PspGI	CCWGG	3 cut(s)	40, 438, 1495
PspOMI	GGGCCC	2 cut(s)	28, 191
PspPI	GGNCC	9 cut(s)	28, 29, 161, 191, 192, 388, 443, 1007, 1537
PspPPI	RGGWCCY	2 cut(s)	443, 1007
PstNI	CAGNNNCTG	2 cut(s)	1187, 1502
PsuI	RGATCY	3 cut(s)	689, 922, 1378
PsyI	GACNNNGTC	1 cut(s)	397
PvuII	CAGCTG	1 cut(s)	253
RsaI	GTAC	1 cut(s)	1428
RsaNI	GTAC	1 cut(s)	1427
RseI	CAYNNNNRTG	2 cut(s)	891, 1419
SaqAI	TTAA	1 cut(s)	1565
Sau96I	GGNCC	9 cut(s)	28, 29, 161, 191, 192, 388, 443, 1007, 1537
SchI	GAGTC	3 cut(s)	193, 407, 632
SduI	GDGCHC	3 cut(s)	32, 195, 806
SfaNI	GCATC	3 cut(s)	817, 1087, 1481
SfcI	CTRYAG	3 cut(s)	1261, 1374, 1548
SfoI	GGCGCC	2 cut(s)	145, 1179
SfuI	TTCGAA	2 cut(s)	123, 983
SgrAI	CRCCGGYG	1 cut(s)	146
SinI	GGWCC	4 cut(s)	388, 443, 1007, 1537
SmaI	CCCGGG	2 cut(s)	165, 190
SmiMI	CAYNNNNRTG	2 cut(s)	891, 1419
SphI	GCATGC	1 cut(s)	1344
Sse9I	AATT	8 cut(s)	319, 347, 520, 739, 867, 946, 979, 1060
SspDI	GGCGCC	2 cut(s)	143, 1177
SspMI	CTAG	3 cut(s)	308, 473, 1212
StyI	CCWWGG	1 cut(s)	13
TaaI	ACNGT	5 cut(s)	57, 227, 624, 699, 1201
TaiI	ACGT	3 cut(s)	275, 901, 1564
TaqI	TCGA	8 cut(s)	59, 123, 411, 551, 714, 983, 1242, 1521
TaqII	GACCGA	1 cut(s)	376
TasI	AATT	8 cut(s)	319, 347, 520, 739, 867, 946, 979, 1060
TauI	GCSGC	5 cut(s)	40, 112, 342, 376, 1449
TfiI	GAWTC	4 cut(s)	125, 659, 1037, 1215
Tru1I	TTAA	1 cut(s)	1565
Tru9I	TTAA	1 cut(s)	1565
TscAI	CASTG	1 cut(s)	1240
TseFI	GTSAC	4 cut(s)	227, 481, 1076, 1201
TseI	GCWGC	7 cut(s)	68, 253, 304, 494, 497, 577, 1543
Tsp45I	GTSAC	4 cut(s)	227, 481, 1076, 1201
TspDTI	ATGAA	4 cut(s)	693, 909, 959, 1140
TspGWI	ACGGA	3 cut(s)	894, 1327, 1340
TspMI	CCCGGG	2 cut(s)	163, 188
TspRI	CASTG	1 cut(s)	1240
Tth111I	GACNNNGTC	1 cut(s)	397
Van91I	CCANNNNNTGG	1 cut(s)	1502
VneI	GTGCAC	1 cut(s)	802
VpaK11BI	GGWCC	4 cut(s)	388, 443, 1007, 1537
XapI	RAATTY	4 cut(s)	319, 347, 946, 979
XceI	RCATGY	1 cut(s)	1344
XcmI	CCANNNNNNNNNTGG	1 cut(s)	446
XmaI	CCCGGG	2 cut(s)	163, 188
XspI	CTAG	3 cut(s)	308, 473, 1212
ZraI	GACGTC	1 cut(s)	273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.