MD13G1068000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011883
Physical Location & Seq
Forward (+)
4668333 .. 4669400
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1068000.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAAGCTGAAAGTTCACCAAGAGTGAGATTGGATCACTCGAAAAAAGAGGGATCCGAAGAACCCCATTTGCGTAGATTTGAGTTAGATCTCTCTGGTAACGATCATAGATTTCATTCGATGAGTGCTCTGGAGATTTTGAGGGAAACTGTTAGAATTCTCCGGTACAATTCGTGGGGGTTTTTGGCGATTGCCATGTTGCTAATCTGCCCAATTTCCGCCGTGCTTTTGTCGAATTTCCTAGTTGATCAGTCACTTGTAAAGAGACTAACCATTAGGATGCTGTTAATTGCAAAGTCCAGTGGACTCCCTTTGAGGGATTTTATCAGTCACTCTTGCCAGAAGTTAGCTGAGATGGCTATCTCGTGTACAATGTGCTTTCCCTTGTACATCACATTATCATTGTTGTCGAAGGCTGCGGTGGTTTACTCCGTGTACTGTAGTTACTCCCTGAAGAAGTTTGATGCATCCCAGTTTTACTTTGTTATGTGCAAGATATGGAGGCGGGTTGTTTCAACTTATATGTGGATGTGTATGGTGGTTGCTGGTAGCGTTGTCTTGTTTTTGGTCCTTCTTCTTGCTGTGTGCAATGCATGTTCGATAGTGGGGTTGTCCCCGAATTATATTGTGTATGCTGCAATGGTGGTTGGGCTCGCCTTCTCGGTCGTTTTTGCCAATGCCATTATTATTTGCAACATTGCTTTTGTGATCTCTGTGTTGGAGGATGTCTCTGGACCAGAGGCATTGGTTCAGTCTGGGGTTTTGATCAAGGGACAAACTCAGGTGGGGCTTCTGATATTTCTTGGATCGAGTATTGGAATGGCGTTTGTTGAGGGATTGTTTGAGCATAGGGTGAAGACTTTGAGTTACGGAGACGGGTCTTCGCGCATTTGGGAAGGGCCTCTTTTGGTGATCATGTATTCGTTTGTGGTGCTTATCGACTCCATGAACAGTGCAGTTTTCTACTTCAGTTGCAGATCTTTGAAGATGGAAACATCTTTGGATGGTGAATCTCATTCGATTTTGGAAACAATAACCTTTTCTACTGAATCAGTTGGCATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

39.4

Weight (kDa)

7.02

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26650 AT1G69430
fragaria_vesca FvH4_5g29140
malus_domestica MD05G1259900.v1.1 MD13G1068000.v1.1 MD16G1066800.v1.1
prunus_persica Prupe.1G280100_v2.0.a1
pyrus_communis pycom13g05980 pycom16g05820
rosa_chinensis RchiOBHm_Chr7g0221801
rosa_laevigata RLG00000002121
rosa_multiflora Rmu_sc0004505.1_g000001
rosa_roxburghii Rroxscaffold_3G00237700
rosa_rugosa Rorug07G0203500
rosa_samantha Rh7AG344500 Rh7BG335200 Rh7CG361800 Rh7DG342200
rosa_wichuraiana Rw7G029210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 886
AciI CCGC 3 cut(s) 219, 419, 505
AclWI GGATC 4 cut(s) 42, 48, 61, 814
AcsI RAATTY 2 cut(s) 156, 235
AcuI CTGAAG 2 cut(s) 473, 952
AfaI GTAC 4 cut(s) 167, 370, 389, 437
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 3 cut(s) 516, 985, 1064
AluBI AGCT 2 cut(s) 8, 350
AluI AGCT 2 cut(s) 8, 350
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 3 cut(s) 259, 733, 867
AlwI GGATC 4 cut(s) 42, 48, 61, 814
AoxI GGCC 1 cut(s) 899
ApeKI GCWGC 2 cut(s) 416, 635
ApoI RAATTY 2 cut(s) 156, 235
AspLEI GCGC 1 cut(s) 888
AspS9I GGNCC 3 cut(s) 568, 734, 899
AsuHPI GGTGA 4 cut(s) 9, 865, 922, 1019
AvaII GGWCC 2 cut(s) 568, 734
BamHI GGATCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 654
BauI CACGAG 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 863, 873
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 403, 622
BccI CCATC 3 cut(s) 349, 982, 998
BceAI ACGGC 1 cut(s) 206
BclI TGATCA 3 cut(s) 247, 765, 912
BcoDI GTCTC 3 cut(s) 259, 733, 867
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 439
BglII AGATCT 2 cut(s) 88, 977
BisI GCNGC 2 cut(s) 417, 636
BlsI GCNGC 2 cut(s) 418, 637
Bme18I GGWCC 2 cut(s) 568, 734
BmgT120I GGNCC 3 cut(s) 568, 734, 899
BmiI GGNNCC 1 cut(s) 55
BmrI ACTGGG 1 cut(s) 466
BmsI GCATC 3 cut(s) 270, 454, 476
BmuI ACTGGG 1 cut(s) 466
BpiI GAAGAC 2 cut(s) 863, 873
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 152
BsaBI GATNNNNATC 1 cut(s) 359
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 2 cut(s) 300, 472
Bse3DI GCAATG 3 cut(s) 595, 645, 696
Bse8I GATNNNNATC 1 cut(s) 359
BseGI GGATG 5 cut(s) 285, 467, 534, 730, 1009
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 3 cut(s) 595, 645, 696
BseMII CTCAG 2 cut(s) 342, 794
BseNI ACTGG 2 cut(s) 300, 472
BseXI GCAGC 2 cut(s) 403, 622
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 1 cut(s) 886
Bsh1285I CGRYCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 901
BsiEI CGRYCG 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 2 cut(s) 598, 786
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 3 cut(s) 259, 733, 867
BsmBI CGTCTC 1 cut(s) 867
BsmFI GGGAC 2 cut(s) 598, 786
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 1 cut(s) 901
Bsp1286I GDGCHC 2 cut(s) 130, 654
Bsp1407I TGTACA 2 cut(s) 368, 387
BspACI CCGC 3 cut(s) 219, 419, 505
BspANI GGCC 1 cut(s) 901
BspCNI CTCAG 2 cut(s) 343, 793
BspFNI CGCG 1 cut(s) 886
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 4 cut(s) 42, 48, 61, 814
BsrDI GCAATG 3 cut(s) 595, 645, 696
BsrGI TGTACA 2 cut(s) 368, 387
BsrI ACTGG 2 cut(s) 300, 472
BssSI CACGAG 1 cut(s) 364
Bst2BI CACGAG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 151, 440, 953
BstAUI TGTACA 2 cut(s) 368, 387
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 351, 780
BstF5I GGATG 5 cut(s) 285, 467, 534, 730, 1009
BstFNI CGCG 1 cut(s) 886
BstHHI GCGC 1 cut(s) 888
BstMAI GTCTC 3 cut(s) 259, 733, 867
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 597
BstSFI CTRYAG 1 cut(s) 439
BstUI CGCG 1 cut(s) 886
BstV1I GCAGC 2 cut(s) 403, 622
BstV2I GAAGAC 2 cut(s) 863, 873
BstX2I RGATCY 3 cut(s) 53, 88, 977
BstYI RGATCY 3 cut(s) 53, 88, 977
BsuRI GGCC 1 cut(s) 901
BtsCI GGATG 5 cut(s) 285, 467, 534, 730, 1009
BtsIMutI CAGTG 2 cut(s) 307, 958
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 888
Cfr13I GGNCC 3 cut(s) 568, 734, 899
Csp6I GTAC 4 cut(s) 166, 369, 388, 436
CviAII CATG 4 cut(s) 196, 594, 916, 946
CviJI RGCY 7 cut(s) 8, 350, 359, 416, 652, 790, 901
CviKI_1 RGCY 7 cut(s) 8, 350, 359, 416, 652, 790, 901
CviQI GTAC 4 cut(s) 166, 369, 388, 436
DdeI CTNAG 2 cut(s) 351, 780
EciI GGCGGA 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 654
Eco47I GGWCC 2 cut(s) 568, 734
Eco57I CTGAAG 2 cut(s) 473, 952
EcoO109I RGGNCCY 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 156
EcoT22I ATGCAT 2 cut(s) 469, 595
EcoT38I GRGCYC 1 cut(s) 654
Esp3I CGTCTC 1 cut(s) 867
FaeI CATG 4 cut(s) 199, 597, 919, 949
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 2 cut(s) 598, 786
FatI CATG 4 cut(s) 195, 593, 915, 945
FauI CCCGC 1 cut(s) 498
FbaI TGATCA 3 cut(s) 247, 765, 912
Fnu4HI GCNGC 2 cut(s) 417, 636
FokI GGATG 5 cut(s) 292, 454, 541, 737, 1016
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 2 cut(s) 417, 636
FspBI CTAG 1 cut(s) 242
GlaI GCGC 1 cut(s) 887
GluI GCNGC 2 cut(s) 417, 636
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 901
HapII CCGG 1 cut(s) 163
HhaI GCGC 1 cut(s) 888
Hin1II CATG 4 cut(s) 199, 597, 919, 949
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HinfI GANTC 4 cut(s) 306, 941, 1010, 1049
HpaII CCGG 1 cut(s) 163
HphI GGTGA 4 cut(s) 9, 865, 922, 1019
Hpy166II GTNNAC 5 cut(s) 17, 305, 369, 427, 436
Hpy188I TCNGA 2 cut(s) 58, 795
Hpy188III TCNNGA 2 cut(s) 131, 732
Hpy8I GTNNAC 5 cut(s) 17, 305, 369, 427, 436
HpyAV CCTTC 4 cut(s) 406, 581, 667, 890
HpyCH4III ACNGT 3 cut(s) 151, 440, 953
HpyCH4V TGCA 9 cut(s) 293, 467, 492, 588, 593, 638, 693, 956, 975
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 2 cut(s) 351, 780
Hsp92II CATG 4 cut(s) 199, 597, 919, 949
HspAI GCGC 1 cut(s) 886
Ksp22I TGATCA 3 cut(s) 247, 765, 912
Lsp1109I GCAGC 2 cut(s) 403, 622
LweI GCATC 3 cut(s) 270, 454, 476
MaeI CTAG 1 cut(s) 242
MaeIII GTNAC 5 cut(s) 98, 252, 329, 443, 866
MboII GAAGA 6 cut(s) 71, 466, 566, 868, 873, 997
MflI RGATCY 3 cut(s) 53, 88, 977
MhlI GDGCHC 2 cut(s) 130, 654
MluCI AATT 6 cut(s) 156, 169, 213, 235, 288, 619
MlyI GAGTC 2 cut(s) 300, 935
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 8 cut(s) 43, 135, 309, 495, 715, 733, 826, 912
Mph1103I ATGCAT 2 cut(s) 469, 595
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 278
MspI CCGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 1059
MvnI CGCG 1 cut(s) 886
MwoI GCNNNNNNNGC 1 cut(s) 356
NlaIII CATG 4 cut(s) 199, 597, 919, 949
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 2 cut(s) 252, 329
NsiI ATGCAT 2 cut(s) 469, 595
NspI RCATGY 1 cut(s) 597
PctI GAATGC 1 cut(s) 1059
PfeI GAWTC 2 cut(s) 1010, 1049
PkrI GCNGC 2 cut(s) 418, 637
PleI GAGTC 2 cut(s) 300, 935
PpsI GAGTC 2 cut(s) 300, 935
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 3 cut(s) 568, 734, 899
PsuI RGATCY 3 cut(s) 53, 88, 977
RsaI GTAC 4 cut(s) 167, 370, 389, 437
RsaNI GTAC 4 cut(s) 166, 369, 388, 436
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 2 cut(s) 417, 636
Sau96I GGNCC 3 cut(s) 568, 734, 899
SchI GAGTC 2 cut(s) 300, 935
SduI GDGCHC 2 cut(s) 130, 654
SetI ASST 4 cut(s) 10, 352, 786, 1040
SfaNI GCATC 3 cut(s) 270, 454, 476
SfcI CTRYAG 1 cut(s) 439
SinI GGWCC 2 cut(s) 568, 734
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 6 cut(s) 156, 169, 213, 235, 288, 619
SsiI CCGC 3 cut(s) 219, 419, 505
SspMI CTAG 1 cut(s) 242
TaaI ACNGT 3 cut(s) 151, 440, 953
TaqI TCGA 8 cut(s) 41, 119, 233, 410, 599, 809, 939, 1019
TaqII GACCGA 1 cut(s) 652
TasI AATT 6 cut(s) 156, 169, 213, 235, 288, 619
TatI WGTACW 3 cut(s) 368, 387, 435
TfiI GAWTC 2 cut(s) 1010, 1049
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 2 cut(s) 307, 958
TseFI GTSAC 2 cut(s) 252, 329
TseI GCWGC 2 cut(s) 416, 635
Tsp45I GTSAC 2 cut(s) 252, 329
TspDTI ATGAA 2 cut(s) 104, 962
TspGWI ACGGA 2 cut(s) 421, 885
TspRI CASTG 2 cut(s) 307, 958
VpaK11BI GGWCC 2 cut(s) 568, 734
XapI RAATTY 2 cut(s) 156, 235
XceI RCATGY 1 cut(s) 597
XspI CTAG 1 cut(s) 242
Zsp2I ATGCAT 2 cut(s) 469, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.