MD16G1066800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011883
Physical Location & Seq
Forward (+)
4712412 .. 4713479
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1066800.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAAGCTGACAGTTCACCCAGAATGAGATTGGATCACTTGAAAAAAGAGGGATCCGAAGAACCCCATTTGCGTAGATTCGAGTTAGAACTCCCCGTTAACGATCACAGGTTTCATTCGATGAGTGCTCTGGAAATTTTGAGAGAAACCGTTAGAATTCTCCGGTACAATTCGTGGGGGTTTTTGGCGATTGCCTTGTTGCTAATCTGCCCGGTTTCCGCCCTGCTTTTGTCGAATTTGGTAGTTGATCATTCACTTGTAAAGAGACTAACCATTAGGATGTTGTTAATTGCAAAGTCCAGTGGACTCCCTTTGAGGGATTTTATCAGTCACTCCTGCCAGAAGTTTGCTGAGATGGCTATTTCGTGTACGATGTGCTTCCCCTTGTACATCACATTGTCGTTGTTGTCAAAAGCCGCGGTGGTTTACTCTGTGTATTGTAGTTACTCCCTGAAGAAGTTTGATGCATCACAGTTTTACTTGGTTCTGCGCAAGATATGGAGGCGGGTTGTTTCAACTTATGTGTGGATGTGTATGGTGGTTGCCGGTTGTGTCGTCTTGTTTTTGGTTCTTCTTCTTGCTGTGTGCAATGCATGTTCGATAGTAGGGTTGTCGCCGAATTATATTGTGTATGCTGCAATGGTGGTCGCGCTCATCTTCTCGGTTGTTTTTGCCAATGCCGTTATTATTTGCAACATTGCTATTGTGATCTCTGTGTTGGAGGATGTCTCGGGACCAGAGGCACTGGTTCAGTCTGGGGTTCTGATCAAGGGCCAGACTCAGGTTGGTCTTCTAATATTTCTTGGGTCGAGTATTGGAATGGCATTCGTCGAGGGATTGTTTGAGCATAGGGTGAAGACTTTGAGCTATGGAGATGGGTCTTCGCGCATTTGGGAAGGCCCTCTTTTGGTGATCATGTATTCGTTTGTGGTGCTTATCGACTCCATGAACAGCGCGGTTTTCTATTTCAGTTGCAGATCTTTTAAGATGGAAACATCTTTGGATGGTGAATCTCATTCCATTTTGGAAACGGTTAACTTTTCTTCTGAATCAGTTGGTATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

39.5

Weight (kDa)

7.54

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26650 AT1G69430
fragaria_vesca FvH4_5g29140
malus_domestica MD05G1259900.v1.1 MD13G1068000.v1.1 MD16G1066800.v1.1
prunus_persica Prupe.1G280100_v2.0.a1
pyrus_communis pycom13g05980 pycom16g05820
rosa_chinensis RchiOBHm_Chr7g0221801
rosa_laevigata RLG00000002121
rosa_multiflora Rmu_sc0004505.1_g000001
rosa_roxburghii Rroxscaffold_3G00237700
rosa_rugosa Rorug07G0203500
rosa_samantha Rh7AG344500 Rh7BG335200 Rh7CG361800 Rh7DG342200
rosa_wichuraiana Rw7G029210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
AccII CGCG 4 cut(s) 419, 650, 886, 956
AciI CCGC 5 cut(s) 219, 417, 419, 505, 956
AclWI GGATC 3 cut(s) 42, 48, 61
AcsI RAATTY 3 cut(s) 135, 156, 235
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 3 cut(s) 167, 370, 389
AfiI CCNNNNNNNGG 3 cut(s) 316, 781, 907
AgsI TTSAA 3 cut(s) 43, 516, 1064
AluBI AGCT 2 cut(s) 8, 867
AluI AGCT 2 cut(s) 8, 867
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 259, 733
AlwI GGATC 3 cut(s) 42, 48, 61
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 2 cut(s) 772, 898
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 3 cut(s) 135, 156, 235
AspLEI GCGC 4 cut(s) 492, 652, 888, 956
AspS9I GGNCC 3 cut(s) 734, 772, 899
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 4 cut(s) 9, 865, 922, 1019
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 1 cut(s) 734
BamHI GGATCC 1 cut(s) 53
BbsI GAAGAC 3 cut(s) 782, 863, 873
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 622
BccI CCATC 4 cut(s) 349, 869, 982, 998
BceAI ACGGC 1 cut(s) 665
BclI TGATCA 3 cut(s) 247, 765, 912
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 259, 733
BglII AGATCT 1 cut(s) 977
BisI GCNGC 2 cut(s) 417, 636
BlsI GCNGC 2 cut(s) 418, 637
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 734
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 3 cut(s) 734, 772, 899
BmiI GGNNCC 2 cut(s) 55, 735
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 454, 476
BpiI GAAGAC 3 cut(s) 782, 863, 873
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpuMI CCSGG 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 781, 907
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 2 cut(s) 300, 750
Bse3DI GCAATG 3 cut(s) 595, 645, 696
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 4 cut(s) 285, 534, 730, 1009
BseLI CCNNNNNNNGG 3 cut(s) 316, 781, 907
BseMI GCAATG 3 cut(s) 595, 645, 696
BseMII CTCAG 2 cut(s) 342, 794
BseNI ACTGG 2 cut(s) 300, 750
BseXI GCAGC 1 cut(s) 622
Bsh1236I CGCG 4 cut(s) 419, 650, 886, 956
BshFI GGCC 2 cut(s) 774, 900
BsiHKAI GWGCWC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 3 cut(s) 163, 212, 546
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 3 cut(s) 316, 781, 907
BsmAI GTCTC 2 cut(s) 259, 733
BsmFI GGGAC 1 cut(s) 747
BsmI GAATGC 1 cut(s) 824
BsnI GGCC 2 cut(s) 774, 900
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
BspACI CCGC 5 cut(s) 219, 417, 419, 505, 956
BspANI GGCC 2 cut(s) 774, 900
BspCNI CTCAG 2 cut(s) 343, 793
BspFNI CGCG 4 cut(s) 419, 650, 886, 956
BspLI GGNNCC 2 cut(s) 55, 735
BspPI GGATC 3 cut(s) 42, 48, 61
BsrDI GCAATG 3 cut(s) 595, 645, 696
BsrFI RCCGGY 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 300, 750
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
Bst4CI ACNGT 4 cut(s) 14, 151, 474, 1033
BstAUI TGTACA 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 351, 780
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 4 cut(s) 285, 534, 730, 1009
BstFNI CGCG 4 cut(s) 419, 650, 886, 956
BstHHI GCGC 4 cut(s) 492, 652, 888, 956
BstKTI GATC 8 cut(s) 37, 56, 106, 250, 711, 768, 915, 980
BstMAI GTCTC 2 cut(s) 259, 733
BstMBI GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 1 cut(s) 210
BstUI CGCG 4 cut(s) 419, 650, 886, 956
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 3 cut(s) 782, 863, 873
BstX2I RGATCY 2 cut(s) 53, 977
BstYI RGATCY 2 cut(s) 53, 977
BsuRI GGCC 2 cut(s) 774, 900
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 4 cut(s) 285, 534, 730, 1009
BtsIMutI CAGTG 2 cut(s) 307, 743
CfoI GCGC 4 cut(s) 492, 652, 888, 956
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 3 cut(s) 734, 772, 899
Cfr42I CCGCGG 1 cut(s) 420
Csp6I GTAC 3 cut(s) 166, 369, 388
CviAII CATG 3 cut(s) 594, 916, 946
CviJI RGCY 6 cut(s) 8, 359, 416, 774, 867, 900
CviKI_1 RGCY 6 cut(s) 8, 359, 416, 774, 867, 900
CviQI GTAC 3 cut(s) 166, 369, 388
DdeI CTNAG 2 cut(s) 351, 780
DpnI GATC 8 cut(s) 36, 55, 105, 249, 710, 767, 914, 979
DpnII GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
EciI GGCGGA 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 734
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 156
EcoT22I ATGCAT 2 cut(s) 469, 595
FaeI CATG 3 cut(s) 597, 919, 949
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 1 cut(s) 747
FatI CATG 3 cut(s) 593, 915, 945
FauI CCCGC 1 cut(s) 498
FbaI TGATCA 3 cut(s) 247, 765, 912
Fnu4HI GCNGC 2 cut(s) 417, 636
FokI GGATG 4 cut(s) 292, 541, 737, 1016
Fsp4HI GCNGC 2 cut(s) 417, 636
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 4 cut(s) 491, 651, 887, 955
GluI GCNGC 2 cut(s) 417, 636
HaeIII GGCC 2 cut(s) 774, 900
HapII CCGG 3 cut(s) 163, 212, 546
HhaI GCGC 4 cut(s) 492, 652, 888, 956
Hin1II CATG 3 cut(s) 597, 919, 949
Hin6I GCGC 4 cut(s) 490, 650, 886, 954
HinP1I GCGC 4 cut(s) 490, 650, 886, 954
HincII GTYRAC 2 cut(s) 100, 1036
HindII GTYRAC 2 cut(s) 100, 1036
HinfI GANTC 6 cut(s) 78, 306, 778, 941, 1010, 1049
HpaI GTTAAC 2 cut(s) 100, 1036
HpaII CCGG 3 cut(s) 163, 212, 546
HphI GGTGA 4 cut(s) 9, 865, 922, 1019
Hpy166II GTNNAC 6 cut(s) 17, 100, 305, 369, 427, 1036
Hpy188I TCNGA 3 cut(s) 58, 765, 1048
Hpy188III TCNNGA 2 cut(s) 131, 732
Hpy8I GTNNAC 6 cut(s) 17, 100, 305, 369, 427, 1036
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 1 cut(s) 890
HpyCH4III ACNGT 4 cut(s) 14, 151, 474, 1033
HpyCH4V TGCA 7 cut(s) 293, 467, 588, 593, 638, 693, 975
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 2 cut(s) 351, 780
Hsp92II CATG 3 cut(s) 597, 919, 949
HspAI GCGC 4 cut(s) 490, 650, 886, 954
Ksp22I TGATCA 3 cut(s) 247, 765, 912
KspAI GTTAAC 2 cut(s) 100, 1036
KspI CCGCGG 1 cut(s) 420
Kzo9I GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 2 cut(s) 454, 476
MaeIII GTNAC 2 cut(s) 329, 443
MalI GATC 8 cut(s) 36, 55, 105, 249, 710, 767, 914, 979
MboI GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
MboII GAAGA 9 cut(s) 71, 466, 563, 566, 649, 782, 868, 873, 1035
MflI RGATCY 2 cut(s) 53, 977
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 6 cut(s) 135, 156, 169, 235, 288, 619
MlyI GAGTC 3 cut(s) 300, 772, 935
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 7 cut(s) 43, 309, 495, 715, 733, 826, 912
Mph1103I ATGCAT 2 cut(s) 469, 595
MseI TTAA 4 cut(s) 99, 287, 984, 1035
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 3 cut(s) 163, 212, 546
MspR9I CCNGG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 824
MvnI CGCG 4 cut(s) 419, 650, 886, 956
MwoI GCNNNNNNNGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 212
NdeII GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
NlaIII CATG 3 cut(s) 597, 919, 949
NlaIV GGNNCC 2 cut(s) 55, 735
NmuCI GTSAC 1 cut(s) 329
NsbI TGCGCA 1 cut(s) 491
NsiI ATGCAT 2 cut(s) 469, 595
NspI RCATGY 1 cut(s) 597
PctI GAATGC 1 cut(s) 824
PfeI GAWTC 3 cut(s) 78, 1010, 1049
PkrI GCNGC 2 cut(s) 418, 637
PleI GAGTC 3 cut(s) 300, 772, 935
PpsI GAGTC 3 cut(s) 300, 772, 935
PspN4I GGNNCC 2 cut(s) 55, 735
PspPI GGNCC 3 cut(s) 734, 772, 899
PsuI RGATCY 2 cut(s) 53, 977
RsaI GTAC 3 cut(s) 167, 370, 389
RsaNI GTAC 3 cut(s) 166, 369, 388
RseI CAYNNNNRTG 1 cut(s) 278
SacII CCGCGG 1 cut(s) 420
SaqAI TTAA 4 cut(s) 99, 287, 984, 1035
SatI GCNGC 2 cut(s) 417, 636
Sau3AI GATC 8 cut(s) 34, 53, 103, 247, 708, 765, 912, 977
Sau96I GGNCC 3 cut(s) 734, 772, 899
SchI GAGTC 3 cut(s) 300, 772, 935
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 1 cut(s) 130
SetI ASST 4 cut(s) 10, 113, 786, 869
SfaNI GCATC 2 cut(s) 454, 476
Sfr303I CCGCGG 1 cut(s) 420
SgrBI CCGCGG 1 cut(s) 420
SinI GGWCC 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 6 cut(s) 135, 156, 169, 235, 288, 619
SsiI CCGC 5 cut(s) 219, 417, 419, 505, 956
SspI AATATT 1 cut(s) 798
StyD4I CCNGG 1 cut(s) 210
TaaI ACNGT 4 cut(s) 14, 151, 474, 1033
TaqI TCGA 7 cut(s) 81, 119, 233, 599, 809, 831, 939
TasI AATT 6 cut(s) 135, 156, 169, 235, 288, 619
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 3 cut(s) 78, 1010, 1049
Tru1I TTAA 4 cut(s) 99, 287, 984, 1035
Tru9I TTAA 4 cut(s) 99, 287, 984, 1035
TscAI CASTG 2 cut(s) 307, 750
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 2 cut(s) 104, 962
TspRI CASTG 2 cut(s) 307, 750
VpaK11BI GGWCC 1 cut(s) 734
XapI RAATTY 3 cut(s) 135, 156, 235
XceI RCATGY 1 cut(s) 597
XcmI CCANNNNNNNNNTGG 2 cut(s) 28, 782
Zsp2I ATGCAT 2 cut(s) 469, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.