MD13G1068600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004473
Physical Location & Seq
Forward (+)
4710626 .. 4713047
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1068600.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGATTCCCTCTCATCGGTAAAAGTGTCGCCCTCCGTAGTCACCAACCCTCTCTCTCCACGGCGCTGCCGGATGCTTCTCTTTTCGTCTTCGCTCGGCAGCTTACACAATCGAAGTCTCGGTCGATTATCCTCCTCCTCCTCCTCCTCCTCCTCTGTATTCGGTGTACCGTGCCCTCCCTATTCTAGAAGGAGGCTCGTGTGCTGGTCCGGCGGTGGCGGTGGCGGTGGAGCTGCGCAGGAGGAGGACGACGACGGAGATGACGACGGAAGTGAGCAGGTGGAGAGAGCGCTTCACCTCGATGGTAATATCCCCTCTTCCTCCGACGAGTTCGTGAAACGCGTGTCGTCTCGTGCTTACGACATGCGCCACCACCTCCAGCAGACCTTCGATTCCAGCAGCTACGATGTATTGGAGGCCAACCCTTGGAGAGGAAGTTCCAAGCCTGTGTATGTATTAACGCAAAGGGAAAACCAGTTGTGCACCATGAAAACCCGAAGAAATCGCAGTGAAGTTGAAACGGAGCTTGGGAAATTGTTTTCGAAAGGAGGGAAATGGAACCCAGCGAAACAGCCTAGGAACGAGACAAAGTTCCAGATGCTTGTGGAGGATATTAGGGATGGAGTGCTCGTTTTCGAGGATGAGAACGAAGCTGTAAGGTACTGTGACTTGTTGCAAGGAGGAGGTAAAGGTTGTGAAGGTGTTGCAGAAATAGAGGCCTCATCGGTATTCGACGTCTGCCAGCAAATGCGAGCTTTTGCAGTTCTATTCCGTCGCGGAATAAGCCCTCCTCTGCCTCAAAGCCTGGAGCTCAACCTTCGGGCTCGAAAGCGCTCGCTTGAAGACGAACAAAACCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3110

Protein Analysis

287

Amino Acids

31.65

Weight (kDa)

6.16

Isoelectric Point (pI)

75.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
AatII GACGTC 1 cut(s) 738
Acc16I TGCGCA 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 268
AccB7I CCANNNNNTGG 1 cut(s) 426
AccII CGCG 2 cut(s) 342, 777
AciI CCGC 4 cut(s) 213, 219, 225, 777
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 2 cut(s) 168, 662
AfeI AGCGCT 2 cut(s) 291, 833
AfiI CCNNNNNNNGG 3 cut(s) 16, 426, 431
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 2 cut(s) 518, 842
AjnI CCWGG 1 cut(s) 804
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
AloI GAACNNNNNNTCC 2 cut(s) 421, 453
AluBI AGCT 7 cut(s) 102, 233, 402, 526, 653, 755, 811
AluI AGCT 7 cut(s) 102, 233, 402, 526, 653, 755, 811
Alw21I GWGCWC 3 cut(s) 485, 630, 813
Alw26I GTCTC 3 cut(s) 122, 354, 578
Alw44I GTGCAC 1 cut(s) 481
Aor51HI AGCGCT 2 cut(s) 291, 833
AoxI GGCC 2 cut(s) 417, 717
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 4 cut(s) 66, 99, 233, 399
ArsI GACNNNNNNTTYG 2 cut(s) 106, 138
AspA2I CCTAGG 1 cut(s) 575
AspLEI GCGC 5 cut(s) 66, 238, 292, 369, 834
AspS9I GGNCC 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 34, 287
AsuII TTCGAA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 207
AvrII CCTAGG 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 176, 485
BanII GRGCYC 2 cut(s) 813, 826
BauI CACGAG 2 cut(s) 197, 351
BbsI GAAGAC 2 cut(s) 81, 849
Bbv12I GWGCWC 3 cut(s) 485, 630, 813
BbvI GCAGC 4 cut(s) 53, 111, 220, 411
BccI CCATC 2 cut(s) 296, 614
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 806
BcoDI GTCTC 3 cut(s) 122, 354, 578
BfaI CTAG 2 cut(s) 186, 576
BfoI RGCGCY 3 cut(s) 67, 293, 835
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 67, 100, 234, 400
BlnI CCTAGG 1 cut(s) 575
BlsI GCNGC 4 cut(s) 68, 101, 235, 401
Bme1390I CCNGG 1 cut(s) 806
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 1 cut(s) 806
BmsI GCATC 2 cut(s) 63, 588
BpiI GAAGAC 2 cut(s) 81, 849
BpmI CTGGAG 2 cut(s) 362, 827
Bpu14I TTCGAA 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 59, 425, 575
BsaXI ACNNNNNCTCC 4 cut(s) 421, 451, 615, 645
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 426, 431
Bse1I ACTGG 1 cut(s) 475
BseBI CCWGG 1 cut(s) 806
BseDI CCNNGG 3 cut(s) 59, 425, 575
BseGI GGATG 3 cut(s) 78, 625, 646
BseLI CCNNNNNNNGG 3 cut(s) 16, 426, 431
BseNI ACTGG 1 cut(s) 475
BseSI GKGCMC 2 cut(s) 176, 485
BseXI GCAGC 4 cut(s) 53, 111, 220, 411
BseYI CCCAGC 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 342, 777
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 2 cut(s) 419, 719
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 3 cut(s) 485, 630, 813
BsiSI CCGG 2 cut(s) 70, 210
BslI CCNNNNNNNGG 3 cut(s) 16, 426, 431
BsmAI GTCTC 3 cut(s) 122, 354, 578
BsmBI CGTCTC 1 cut(s) 354
BsnI GGCC 2 cut(s) 419, 719
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 5 cut(s) 176, 485, 630, 813, 826
BspACI CCGC 4 cut(s) 213, 219, 225, 777
BspANI GGCC 2 cut(s) 419, 719
BspFNI CGCG 2 cut(s) 342, 777
BspLI GGNNCC 1 cut(s) 560
BspMI ACCTGC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 542
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 3 cut(s) 59, 425, 575
BssNI GRCGYC 1 cut(s) 735
BssSI CACGAG 2 cut(s) 197, 351
BssT1I CCWWGG 2 cut(s) 425, 575
Bst2BI CACGAG 2 cut(s) 197, 351
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 2 cut(s) 171, 665
Bst6I CTCTTC 1 cut(s) 322
BstACI GRCGYC 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 743, 753, 836
BstDSI CCRYGG 1 cut(s) 59
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 3 cut(s) 78, 625, 646
BstFNI CGCG 2 cut(s) 342, 777
BstH2I RGCGCY 3 cut(s) 67, 293, 835
BstHHI GCGC 5 cut(s) 66, 238, 292, 369, 834
BstMAI GTCTC 3 cut(s) 122, 354, 578
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 210, 783
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 804
BstSLI GKGCMC 2 cut(s) 176, 485
BstUI CGCG 2 cut(s) 342, 777
BstV1I GCAGC 4 cut(s) 53, 111, 220, 411
BstV2I GAAGAC 2 cut(s) 81, 849
BsuRI GGCC 2 cut(s) 419, 719
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 78, 625, 646
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 3 cut(s) 743, 753, 836
CfoI GCGC 5 cut(s) 66, 238, 292, 369, 834
Cfr13I GGNCC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 167, 661
CviAII CATG 2 cut(s) 364, 487
CviQI GTAC 2 cut(s) 167, 661
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Ecl136II GAGCTC 1 cut(s) 811
Eco130I CCWWGG 2 cut(s) 425, 575
Eco147I AGGCCT 1 cut(s) 719
Eco24I GRGCYC 2 cut(s) 813, 826
Eco47I GGWCC 1 cut(s) 207
Eco47III AGCGCT 2 cut(s) 291, 833
Eco53kI GAGCTC 1 cut(s) 811
EcoICRI GAGCTC 1 cut(s) 811
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 804
EcoT14I CCWWGG 2 cut(s) 425, 575
EcoT38I GRGCYC 2 cut(s) 813, 826
ErhI CCWWGG 2 cut(s) 425, 575
Esp3I CGTCTC 1 cut(s) 354
FaeI CATG 2 cut(s) 367, 490
FaiI YATR 4 cut(s) 365, 453, 488, 859
FatI CATG 2 cut(s) 363, 486
Fnu4HI GCNGC 4 cut(s) 67, 100, 234, 400
FokI GGATG 3 cut(s) 85, 632, 653
FriOI GRGCYC 2 cut(s) 813, 826
Fsp4HI GCNGC 4 cut(s) 67, 100, 234, 400
FspBI CTAG 2 cut(s) 186, 576
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 5 cut(s) 65, 237, 291, 368, 833
GluI GCNGC 4 cut(s) 67, 100, 234, 400
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 2 cut(s) 362, 827
HaeII RGCGCY 3 cut(s) 67, 293, 835
HaeIII GGCC 2 cut(s) 419, 719
HapII CCGG 2 cut(s) 70, 210
HhaI GCGC 5 cut(s) 66, 238, 292, 369, 834
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 2 cut(s) 367, 490
Hin6I GCGC 5 cut(s) 64, 236, 290, 367, 832
HinP1I GCGC 5 cut(s) 64, 236, 290, 367, 832
HinfI GANTC 2 cut(s) 5, 392
HpaII CCGG 2 cut(s) 70, 210
HphI GGTGA 2 cut(s) 34, 287
Hpy166II GTNNAC 2 cut(s) 167, 483
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 3 cut(s) 186, 334, 595
Hpy8I GTNNAC 2 cut(s) 167, 483
Hpy99I CGWCG 6 cut(s) 254, 257, 269, 329, 737, 777
HpyAV CCTTC 4 cut(s) 183, 397, 692, 827
HpyCH4III ACNGT 2 cut(s) 171, 665
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 4 cut(s) 483, 676, 707, 761
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 783
HpySE526I ACGT 1 cut(s) 735
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 2 cut(s) 367, 490
HspAI GCGC 5 cut(s) 64, 236, 290, 367, 832
LmnI GCTCC 3 cut(s) 230, 523, 808
Lsp1109I GCAGC 4 cut(s) 53, 111, 220, 411
LweI GCATC 2 cut(s) 63, 588
MaeI CTAG 2 cut(s) 186, 576
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 2 cut(s) 40, 665
MboII GAAGA 4 cut(s) 81, 309, 510, 854
MhlI GDGCHC 5 cut(s) 176, 485, 630, 813, 826
MluCI AATT 1 cut(s) 533
MluI ACGCGT 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 348
MseI TTAA 1 cut(s) 458
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 2 cut(s) 70, 210
MspR9I CCNGG 1 cut(s) 806
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 2 cut(s) 342, 777
MwoI GCNNNNNNNGC 2 cut(s) 210, 783
NlaIII CATG 2 cut(s) 367, 490
NlaIV GGNNCC 1 cut(s) 560
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 2 cut(s) 40, 665
NsbI TGCGCA 1 cut(s) 237
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 542
PaqCI CACCTGC 1 cut(s) 268
PceI AGGCCT 1 cut(s) 719
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 339
PfeI GAWTC 2 cut(s) 5, 392
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 4 cut(s) 68, 101, 235, 401
Psp124BI GAGCTC 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 804
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 207
RsaI GTAC 2 cut(s) 168, 662
RsaNI GTAC 2 cut(s) 167, 661
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 813
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 4 cut(s) 67, 100, 234, 400
Sau96I GGNCC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 806
SduI GDGCHC 5 cut(s) 176, 485, 630, 813, 826
SfaNI GCATC 2 cut(s) 63, 588
SfuI TTCGAA 1 cut(s) 542
SinI GGWCC 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 1 cut(s) 533
SseBI AGGCCT 1 cut(s) 719
SsiI CCGC 4 cut(s) 213, 219, 225, 777
SspMI CTAG 2 cut(s) 186, 576
SstI GAGCTC 1 cut(s) 813
StuI AGGCCT 1 cut(s) 719
StyD4I CCNGG 1 cut(s) 804
StyI CCWWGG 2 cut(s) 425, 575
TaaI ACNGT 2 cut(s) 171, 665
TaiI ACGT 1 cut(s) 738
TaqI TCGA 8 cut(s) 112, 124, 300, 390, 542, 636, 732, 826
TaqII GACCGA 1 cut(s) 110
TasI AATT 1 cut(s) 533
TfiI GAWTC 2 cut(s) 5, 392
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 2 cut(s) 40, 665
TseI GCWGC 4 cut(s) 66, 99, 233, 399
Tsp45I GTSAC 2 cut(s) 40, 665
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 5 cut(s) 25, 270, 282, 536, 761
TspRI CASTG 1 cut(s) 514
Van91I CCANNNNNTGG 1 cut(s) 426
VneI GTGCAC 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 207
XagI CCTNNNNNAGG 1 cut(s) 429
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 1 cut(s) 367
XmaJI CCTAGG 1 cut(s) 575
XspI CTAG 2 cut(s) 186, 576
ZraI GACGTC 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.