Rroxscaffold_5G00380140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004473
Physical Location & Seq
Forward (+)
60308529 .. 60315829
7301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380140.1

Sequence Viewer

Length: 843 bp
ATGAGTGAGGTCTTCGAAGGCTACGAACGTCAGTACTGTGAGCTCTCCGCCAACCTCTCCCGGAAATGTAACGCCGCCGCTCTTCTGCCTGACCAAGGGCAGAAGCAGCAGAGGTTTTCTGAGATTAGAGCTGGATTGGATGATGCTGATGGTTTGATTCGGAAAATGGACCTGGAAGCAAGAAGCTTGCAGCCGGGGGCAAAGGCGGTGCTGCTTGCAAAGTTGAGGGAGTACAAATCTGATCTCAATAATTGTGTGCATTTACTCCTTAATCTCAGTGGCAATATCATGAGTGACTCGTCAGTTTACGTTAAGCAAATTAACGATCGAGGCTATGAACTCAGAAGTCACCTTCTACCTTGCCAAGGAAAGTCTACGATGTTAGATTGGGTAAATGAGATGAACAGTGTTTGTGAAGCGCCTATGATGCCTGTATATGTATTAACTCAGAGGGAGAACCAGCTGTGCACAATGAAAACACGAAGAAATGTCAGGTCTTTGAAAGGGGAGCTCGGGCAATTGTTTTCCAAAGGAGGAAAGTGGAACCAAGCTAAACAGCCCAGAAATGGTAACAGGTTCCAGATGCTTGTTGAGGATATTAGGGATGGAGTGCTTGTTTTCGAGGATGCAAATGAAGCAGTGAAGTACTGTGATTTGTTGCAAGGAGGCGGCCAAGGTTGTGAAGGTGTTGCAGAAATAGAGGCCTCATCAGTATTTGCTTTGTGCCAGAAAATGAGAGGTCTAGCGGTTCTGTTTCGTCGCGGAAGAAAGCCCCCGTTGCCCCAAAGCTTGGAGCTCAACCTTAGGGCCAGGAAGCGCTCCCTTGAAGACCAACAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3110

Protein Analysis

280

Amino Acids

31.58

Weight (kDa)

8.68

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE PF05008 1 - 84 1.9e-24 Vesicle transport v-SNARE protein N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 374
AccII CGCG 1 cut(s) 762
AciI CCGC 7 cut(s) 48, 75, 78, 206, 669, 746, 762
AcoI YGGCCR 1 cut(s) 670
AfaI GTAC 3 cut(s) 35, 233, 647
AfeI AGCGCT 1 cut(s) 818
AfiI CCNNNNNNNGG 4 cut(s) 95, 365, 566, 790
AgsI TTSAA 2 cut(s) 502, 827
AjnI CCWGG 2 cut(s) 171, 809
AluBI AGCT 8 cut(s) 43, 131, 186, 463, 511, 551, 789, 796
AluI AGCT 8 cut(s) 43, 131, 186, 463, 511, 551, 789, 796
Alw21I GWGCWC 4 cut(s) 45, 470, 513, 798
Alw44I GTGCAC 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 512
Aor51HI AGCGCT 1 cut(s) 818
AoxI GGCC 3 cut(s) 670, 702, 807
ApaLI GTGCAC 1 cut(s) 466
ApeKI GCWGC 3 cut(s) 106, 190, 211
AspLEI GCGC 2 cut(s) 421, 819
AspS9I GGNCC 2 cut(s) 169, 807
AsuC2I CCSGG 2 cut(s) 61, 195
AsuHPI GGTGA 1 cut(s) 341
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 803
BaeGI GKGCMC 1 cut(s) 470
BanII GRGCYC 3 cut(s) 45, 513, 798
BbsI GAAGAC 2 cut(s) 4, 834
Bbv12I GWGCWC 4 cut(s) 45, 470, 513, 798
BbvI GCAGC 3 cut(s) 118, 198, 202
BccI CCATC 2 cut(s) 143, 599
BciT130I CCWGG 2 cut(s) 173, 811
BcnI CCSGG 2 cut(s) 61, 195
BfaI CTAG 1 cut(s) 743
BfoI RGCGCY 2 cut(s) 422, 820
BisI GCNGC 6 cut(s) 75, 78, 107, 191, 212, 670
BlsI GCNGC 6 cut(s) 76, 79, 108, 192, 213, 671
BmcAI AGTACT 2 cut(s) 35, 647
Bme1390I CCNGG 4 cut(s) 61, 173, 195, 811
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 169, 807
BmiI GGNNCC 2 cut(s) 545, 578
BmrFI CCNGG 4 cut(s) 61, 173, 195, 811
BmsI GCATC 4 cut(s) 133, 417, 573, 616
BpiI GAAGAC 2 cut(s) 4, 834
Bpu14I TTCGAA 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 61, 195
BsaJI CCNNGG 4 cut(s) 94, 194, 364, 673
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 365, 566, 790
Bse21I CCTNAGG 1 cut(s) 803
BseBI CCWGG 2 cut(s) 173, 811
BseDI CCNNGG 4 cut(s) 94, 194, 364, 673
BseGI GGATG 3 cut(s) 145, 610, 631
BseLI CCNNNNNNNGG 4 cut(s) 95, 365, 566, 790
BseMII CTCAG 4 cut(s) 111, 289, 355, 461
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 3 cut(s) 118, 198, 202
Bsh1236I CGCG 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 672, 704, 809
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 4 cut(s) 45, 470, 513, 798
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 2 cut(s) 61, 194
BslI CCNNNNNNNGG 4 cut(s) 95, 365, 566, 790
BsnI GGCC 3 cut(s) 672, 704, 809
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 4 cut(s) 45, 470, 513, 798
Bsp143I GATC 2 cut(s) 241, 325
BspACI CCGC 7 cut(s) 48, 75, 78, 206, 669, 746, 762
BspANI GGCC 3 cut(s) 672, 704, 809
BspCNI CTCAG 4 cut(s) 112, 288, 354, 460
BspFNI CGCG 1 cut(s) 762
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 545, 578
BspQI GCTCTTC 1 cut(s) 87
BspT104I TTCGAA 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 80
BssECI CCNNGG 4 cut(s) 94, 194, 364, 673
BssMI GATC 2 cut(s) 241, 325
BssT1I CCWWGG 3 cut(s) 94, 364, 673
Bst2UI CCWGG 2 cut(s) 173, 811
Bst4CI ACNGT 3 cut(s) 38, 407, 650
Bst6I CTCTTC 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 188, 216
BstDEI CTNAG 5 cut(s) 120, 275, 341, 447, 803
BstENI CCTNNNNNAGG 2 cut(s) 93, 363
BstF5I GGATG 3 cut(s) 145, 610, 631
BstFNI CGCG 1 cut(s) 762
BstH2I RGCGCY 2 cut(s) 422, 820
BstHHI GCGC 2 cut(s) 421, 819
BstKTI GATC 2 cut(s) 244, 328
BstMBI GATC 2 cut(s) 241, 325
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 106, 427, 635, 778
BstNI CCWGG 2 cut(s) 173, 811
BstSCI CCNGG 4 cut(s) 59, 171, 193, 809
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 3 cut(s) 118, 198, 202
BstV2I GAAGAC 2 cut(s) 4, 834
Bsu36I CCTNAGG 1 cut(s) 803
BsuRI GGCC 3 cut(s) 672, 704, 809
BtsCI GGATG 3 cut(s) 145, 610, 631
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 283, 412, 645
Cac8I GCNNGC 2 cut(s) 188, 216
CciI TCATGA 1 cut(s) 288
CfoI GCGC 2 cut(s) 421, 819
Cfr13I GGNCC 2 cut(s) 169, 807
Csp6I GTAC 3 cut(s) 34, 232, 646
CviAII CATG 1 cut(s) 289
CviQI GTAC 3 cut(s) 34, 232, 646
DdeI CTNAG 5 cut(s) 120, 275, 341, 447, 803
DpnI GATC 2 cut(s) 243, 327
DpnII GATC 2 cut(s) 241, 325
EaeI YGGCCR 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 3 cut(s) 43, 511, 796
Eco130I CCWWGG 3 cut(s) 94, 364, 673
Eco147I AGGCCT 1 cut(s) 704
Eco24I GRGCYC 3 cut(s) 45, 513, 798
Eco47I GGWCC 1 cut(s) 169
Eco47III AGCGCT 1 cut(s) 818
Eco53kI GAGCTC 3 cut(s) 43, 511, 796
Eco81I CCTNAGG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 3 cut(s) 43, 511, 796
EcoNI CCTNNNNNAGG 2 cut(s) 93, 363
EcoRII CCWGG 2 cut(s) 171, 809
EcoT14I CCWWGG 3 cut(s) 94, 364, 673
EcoT38I GRGCYC 3 cut(s) 45, 513, 798
ErhI CCWWGG 3 cut(s) 94, 364, 673
FaeI CATG 1 cut(s) 292
FaiI YATR 5 cut(s) 290, 336, 425, 436, 438
FatI CATG 1 cut(s) 288
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 6 cut(s) 75, 78, 107, 191, 212, 670
FokI GGATG 3 cut(s) 152, 617, 638
FriOI GRGCYC 3 cut(s) 45, 513, 798
Fsp4HI GCNGC 6 cut(s) 75, 78, 107, 191, 212, 670
FspBI CTAG 1 cut(s) 743
GlaI GCGC 2 cut(s) 420, 818
GluI GCNGC 6 cut(s) 75, 78, 107, 191, 212, 670
HaeII RGCGCY 2 cut(s) 422, 820
HaeIII GGCC 3 cut(s) 672, 704, 809
HapII CCGG 2 cut(s) 61, 194
HhaI GCGC 2 cut(s) 421, 819
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 2 cut(s) 419, 817
HinP1I GCGC 2 cut(s) 419, 817
HindIII AAGCTT 2 cut(s) 184, 787
HinfI GANTC 2 cut(s) 157, 296
HpaII CCGG 2 cut(s) 61, 194
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 3 cut(s) 307, 375, 468
Hpy188I TCNGA 5 cut(s) 121, 162, 241, 344, 450
Hpy188III TCNNGA 2 cut(s) 289, 580
Hpy8I GTNNAC 3 cut(s) 307, 375, 468
Hpy99I CGWCG 1 cut(s) 762
HpyAV CCTTC 3 cut(s) 11, 362, 677
HpyCH4III ACNGT 3 cut(s) 38, 407, 650
HpyCH4IV ACGT 2 cut(s) 28, 309
HpyCH4V TGCA 7 cut(s) 190, 218, 259, 468, 629, 661, 692
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 427, 635, 778
HpyF3I CTNAG 5 cut(s) 120, 275, 341, 447, 803
HpySE526I ACGT 2 cut(s) 28, 309
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 2 cut(s) 419, 817
Kzo9I GATC 2 cut(s) 241, 325
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 3 cut(s) 508, 793, 824
Lsp1109I GCAGC 3 cut(s) 118, 198, 202
LweI GCATC 4 cut(s) 133, 417, 573, 616
MaeI CTAG 1 cut(s) 743
MaeII ACGT 2 cut(s) 28, 309
MaeIII GTNAC 4 cut(s) 68, 293, 347, 569
MalI GATC 2 cut(s) 243, 327
MbiI CCGCTC 1 cut(s) 80
MboI GATC 2 cut(s) 241, 325
MboII GAAGA 5 cut(s) 4, 74, 495, 777, 839
MfeI CAATTG 1 cut(s) 518
MhlI GDGCHC 4 cut(s) 45, 470, 513, 798
MluCI AATT 3 cut(s) 250, 318, 518
MlyI GAGTC 1 cut(s) 290
MseI TTAA 4 cut(s) 270, 312, 321, 443
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 2 cut(s) 61, 194
MspR9I CCNGG 4 cut(s) 61, 173, 195, 811
MunI CAATTG 1 cut(s) 518
MvaI CCWGG 2 cut(s) 173, 811
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 4 cut(s) 106, 427, 635, 778
NciI CCSGG 2 cut(s) 61, 195
NdeII GATC 2 cut(s) 241, 325
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 2 cut(s) 545, 578
NmuCI GTSAC 2 cut(s) 293, 347
NspV TTCGAA 1 cut(s) 15
PagI TCATGA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 790
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 6 cut(s) 76, 79, 108, 192, 213, 671
Ple19I CGATCG 1 cut(s) 328
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp124BI GAGCTC 3 cut(s) 45, 513, 798
Psp6I CCWGG 2 cut(s) 171, 809
PspGI CCWGG 2 cut(s) 171, 809
PspN4I GGNNCC 2 cut(s) 545, 578
PspPI GGNCC 2 cut(s) 169, 807
PvuI CGATCG 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 3 cut(s) 35, 233, 647
RsaNI GTAC 3 cut(s) 34, 232, 646
SacI GAGCTC 3 cut(s) 45, 513, 798
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 4 cut(s) 270, 312, 321, 443
SatI GCNGC 6 cut(s) 75, 78, 107, 191, 212, 670
Sau3AI GATC 2 cut(s) 241, 325
Sau96I GGNCC 2 cut(s) 169, 807
ScaI AGTACT 2 cut(s) 35, 647
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 4 cut(s) 61, 173, 195, 811
SduI GDGCHC 4 cut(s) 45, 470, 513, 798
SfaNI GCATC 4 cut(s) 133, 417, 573, 616
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 1 cut(s) 169
Sse9I AATT 3 cut(s) 250, 318, 518
SseBI AGGCCT 1 cut(s) 704
SsiI CCGC 7 cut(s) 48, 75, 78, 206, 669, 746, 762
SspMI CTAG 1 cut(s) 743
SstI GAGCTC 3 cut(s) 45, 513, 798
StuI AGGCCT 1 cut(s) 704
StyD4I CCNGG 4 cut(s) 59, 171, 193, 809
StyI CCWWGG 3 cut(s) 94, 364, 673
TaaI ACNGT 3 cut(s) 38, 407, 650
TaiI ACGT 2 cut(s) 31, 312
TaqI TCGA 3 cut(s) 15, 328, 621
TasI AATT 3 cut(s) 250, 318, 518
TatI WGTACW 3 cut(s) 33, 231, 645
TauI GCSGC 3 cut(s) 77, 80, 672
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 4 cut(s) 270, 312, 321, 443
Tru9I TTAA 4 cut(s) 270, 312, 321, 443
TscAI CASTG 3 cut(s) 283, 412, 645
TseFI GTSAC 2 cut(s) 293, 347
TseI GCWGC 3 cut(s) 106, 190, 211
Tsp45I GTSAC 2 cut(s) 293, 347
TspDTI ATGAA 4 cut(s) 351, 416, 488, 648
TspRI CASTG 3 cut(s) 283, 412, 645
Van91I CCANNNNNTGG 1 cut(s) 790
VneI GTGCAC 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 169
XagI CCTNNNNNAGG 2 cut(s) 93, 363
XmiI GTMKAC 1 cut(s) 374
XspI CTAG 1 cut(s) 743
ZrmI AGTACT 2 cut(s) 35, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.