MD13G1070100.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011785
Physical Location & Seq
Forward (+)
4898355 .. 4901589
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1070100.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGGTCCAGCAGAGCCATGCTGGCGAACTAATACTAGCTTCTCACCACCCCCATCAAGATGGGATTTCCGTTTCCAACCTGAAGAATTACAGTACGGTTTTCATGATGGTATTCAGCAATATGGGTCTTCTACTTCATCAAACAGTAAAGGAAGTAGGGGCTGGGTGAGAGGCAACAATCTCTACAATCACCACTACTCAGCTTCTGATGGTGCTGGGCTGTTTTTAAGTGGTTCATCTGACCTTTCTCAAGGTCCTCAATGGACACCTCCAGCAATACAGGAAATCAGTGTAGATGATTATGAAACTGCAAAAAAGAGAGGTACAGCTTTGGGGCATACATCCTTTAGACCTACCATGGAGGGAACCTCAGAAATCCAAGACATGGGGGGCTCCACCTCATCCCGTTCAGACAGTGGCGAGTCTGAACCCACTGTCAAGCCGTACCTATCTTCTCATCATACATATGCAAGTCGGCGTTCGTTCATGTCCAAACCTATTCATCCCTTGTCCTTTCCTGCACAAACTTCCCCCAGAGAGTCTTCTGATCTCACAGTTGCTGGGTTTCCAGAGTATGATTCTGCCACTCCTCAAAGAGATGGCCACCGCTGGAGTAGTGCCAGTAGCAGCATTGATTTTGCAGATGTTTCTGAGTCATTTGAGGCAGAGAGTTCTGGTCGACCTTGTAATAATGTGTCTGATGGTTTTAGATGTGGCTTGTGTGAAAGGTTTCTTTCACAGAGATCGCCTTGGAGTTCTCGTCGGATAGTGAGAAGTGGAGATATGCCAGTTACCGGGGTTCTTTCTTGCTCTCATGTTTTCCATGCAGAGTGCTTGGAGCAAACAACACCCAAGACACGAAAAAGCGACCCTCCCTGTCCCCTATGTGCCAGATTTGAGGAAGAAAATCTCCAAGAACAGCAAGGCTTCTCAAGATTGCGGGCTGGTTTTCCAAGGCTTAGACCAATTAGTGATGATGGGCCATCCAGGCCTTGGGGCTGTGCACAGGTGGGAGATTGCGTTGAAGGGGCTTTGCATGCTCCCCCGCGCAATAGTATGCTGTTGCTTAACAGGAGTCGCATAAAGAAAAATCTTTCTTTGAAAGGTAATTTGGGCAAAGAGTTTCCAGGTAAGCTAAGAAAAACTGGTTCTCACTCTTTGCAGCATTTCAGTGGAAAATCAGTCGACCAGGGAGCGGTTGGAAGCTCAAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

406

Amino Acids

44.41

Weight (kDa)

8.3

Isoelectric Point (pI)

60.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14180 AT1G14180
fragaria_vesca FvH4_4g31130 FvH4_4g31130
malus_domestica MD13G1070100.v1.1 MD16G1070500.v1.1
prunus_persica Prupe.1G276800_v2.0.a1
pyrus_communis pycom13g06280 pycom16g06130
rosa_chinensis RchiOBHm_Chr4g0439521
rosa_laevigata RLG00000006261
rosa_multiflora Rmu_sc0007276.1_g000009
rosa_roxburghii Rroxscaffold_5G00380440
rosa_rugosa Rorug04G0319300
rosa_samantha Rh4AG370400 Rh4BG382300 Rh4CG396900 Rh4DG377000
rosa_wichuraiana Rw4G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 385, 993
AccBSI CCGCTC 1 cut(s) 1196
AccI GTMKAC 2 cut(s) 679, 1185
AccII CGCG 1 cut(s) 1048
AciI CCGC 4 cut(s) 607, 940, 1046, 1196
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 3 cut(s) 95, 325, 446
AfiI CCNNNNNNNGG 4 cut(s) 385, 794, 993, 1195
AgsI TTSAA 2 cut(s) 1025, 1102
AjnI CCWGG 3 cut(s) 986, 1126, 1188
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AluBI AGCT 5 cut(s) 39, 203, 329, 1135, 1206
AluI AGCT 5 cut(s) 39, 203, 329, 1135, 1206
Alw21I GWGCWC 1 cut(s) 1006
Alw44I GTGCAC 1 cut(s) 1002
AlwNI CAGNNNCTG 2 cut(s) 206, 560
AoxI GGCC 3 cut(s) 601, 980, 989
ApaLI GTGCAC 1 cut(s) 1002
ApeKI GCWGC 2 cut(s) 627, 1162
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 1 cut(s) 1050
AspS9I GGNCC 3 cut(s) 5, 254, 980
AsuC2I CCSGG 1 cut(s) 796
AsuHPI GGTGA 3 cut(s) 36, 178, 182
AvaII GGWCC 2 cut(s) 5, 254
BaeGI GKGCMC 1 cut(s) 1006
BalI TGGCCA 1 cut(s) 603
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 2 cut(s) 120, 534
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 2 cut(s) 639, 1174
BccI CCATC 8 cut(s) 54, 61, 101, 203, 593, 695, 971, 991
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 3 cut(s) 988, 1128, 1190
BcnI CCSGG 1 cut(s) 796
BfaI CTAG 1 cut(s) 36
BglI GCCNNNNNGGC 2 cut(s) 22, 988
BisI GCNGC 2 cut(s) 628, 1163
BlsI GCNGC 2 cut(s) 629, 1164
Bme1390I CCNGG 4 cut(s) 796, 988, 1128, 1190
Bme18I GGWCC 2 cut(s) 5, 254
BmgT120I GGNCC 3 cut(s) 5, 254, 980
BmiI GGNNCC 3 cut(s) 6, 367, 394
BmrFI CCNGG 4 cut(s) 796, 988, 1128, 1190
BpiI GAAGAC 2 cut(s) 120, 534
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 2 cut(s) 255, 631
BpuEI CTTGAG 2 cut(s) 234, 916
BpuMI CCSGG 1 cut(s) 796
BsaJI CCNNGG 6 cut(s) 357, 749, 795, 953, 992, 1189
BsaXI ACNNNNNCTCC 4 cut(s) 745, 775, 1005, 1035
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 794, 993, 1195
Bse1I ACTGG 3 cut(s) 621, 788, 1150
BseBI CCWGG 3 cut(s) 988, 1128, 1190
BseDI CCNNGG 6 cut(s) 357, 749, 795, 953, 992, 1189
BseGI GGATG 4 cut(s) 341, 401, 502, 983
BseLI CCNNNNNNNGG 4 cut(s) 385, 794, 993, 1195
BseMII CTCAG 3 cut(s) 213, 384, 642
BseNI ACTGG 3 cut(s) 621, 788, 1150
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 1006
BseXI GCAGC 2 cut(s) 639, 1174
BseYI CCCAGC 3 cut(s) 162, 215, 560
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 1048
BshFI GGCC 3 cut(s) 603, 982, 991
BsiHKAI GWGCWC 1 cut(s) 1006
BsiSI CCGG 1 cut(s) 795
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 4 cut(s) 385, 794, 993, 1195
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 3 cut(s) 603, 982, 991
Bsp1286I GDGCHC 2 cut(s) 395, 1006
Bsp143I GATC 2 cut(s) 547, 743
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 4 cut(s) 607, 940, 1046, 1196
BspANI GGCC 3 cut(s) 603, 982, 991
BspCNI CTCAG 3 cut(s) 212, 383, 643
BspFNI CGCG 1 cut(s) 1048
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 6, 367, 394
BsrBI CCGCTC 1 cut(s) 1196
BsrI ACTGG 3 cut(s) 621, 788, 1150
BssECI CCNNGG 6 cut(s) 357, 749, 795, 953, 992, 1189
BssMI GATC 2 cut(s) 547, 743
BssT1I CCWWGG 4 cut(s) 357, 749, 953, 992
Bst2UI CCWGG 3 cut(s) 988, 1128, 1190
Bst4CI ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
BstC8I GCNNGC 3 cut(s) 23, 942, 1038
BstDEI CTNAG 5 cut(s) 199, 370, 651, 959, 1136
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 4 cut(s) 341, 401, 502, 983
BstFNI CGCG 1 cut(s) 1048
BstHHI GCGC 1 cut(s) 1050
BstKTI GATC 2 cut(s) 550, 746
BstMBI GATC 2 cut(s) 547, 743
BstMWI GCNNNNNNNGC 3 cut(s) 22, 988, 1037
BstNI CCWGG 3 cut(s) 988, 1128, 1190
BstNSI RCATGY 1 cut(s) 1040
BstSCI CCNGG 4 cut(s) 794, 986, 1126, 1188
BstSLI GKGCMC 1 cut(s) 1006
BstUI CGCG 1 cut(s) 1048
BstV1I GCAGC 2 cut(s) 639, 1174
BstV2I GAAGAC 2 cut(s) 120, 534
BstXI CCANNNNNNTGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 603, 982, 991
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 4 cut(s) 341, 401, 502, 983
BtsIMutI CAGTG 4 cut(s) 295, 421, 432, 1177
Cac8I GCNNGC 3 cut(s) 23, 942, 1038
CaiI CAGNNNCTG 2 cut(s) 206, 560
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 1050
Cfr13I GGNCC 3 cut(s) 5, 254, 980
Csp6I GTAC 3 cut(s) 94, 324, 445
CviAII CATG 8 cut(s) 18, 104, 358, 385, 487, 815, 824, 1037
CviQI GTAC 3 cut(s) 94, 324, 445
DdeI CTNAG 5 cut(s) 199, 370, 651, 959, 1136
DpnI GATC 2 cut(s) 549, 745
DpnII GATC 2 cut(s) 547, 743
EaeI YGGCCR 1 cut(s) 601
Eco130I CCWWGG 4 cut(s) 357, 749, 953, 992
Eco147I AGGCCT 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 5, 254
Eco57I CTGAAG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 986, 1126, 1188
EcoT14I CCWWGG 4 cut(s) 357, 749, 953, 992
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 4 cut(s) 357, 749, 953, 992
FaeI CATG 8 cut(s) 21, 107, 361, 388, 490, 818, 827, 1040
FaqI GGGAC 1 cut(s) 864
FatI CATG 8 cut(s) 17, 103, 357, 384, 486, 814, 823, 1036
FauI CCCGC 2 cut(s) 933, 1053
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 2 cut(s) 679, 1185
Fnu4HI GCNGC 2 cut(s) 628, 1163
FokI GGATG 4 cut(s) 328, 388, 489, 970
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 628, 1163
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 1049
GluI GCNGC 2 cut(s) 628, 1163
GsaI CCCAGC 3 cut(s) 166, 219, 564
GsuI CTGGAG 2 cut(s) 255, 631
HaeIII GGCC 3 cut(s) 603, 982, 991
HapII CCGG 1 cut(s) 795
HhaI GCGC 1 cut(s) 1050
Hin1II CATG 8 cut(s) 21, 107, 361, 388, 490, 818, 827, 1040
Hin6I GCGC 1 cut(s) 1048
HinP1I GCGC 1 cut(s) 1048
HincII GTYRAC 2 cut(s) 680, 1186
HindII GTYRAC 2 cut(s) 680, 1186
HinfI GANTC 5 cut(s) 422, 539, 578, 653, 1075
HpaII CCGG 1 cut(s) 795
HphI GGTGA 3 cut(s) 36, 178, 182
Hpy166II GTNNAC 3 cut(s) 680, 1004, 1186
Hpy188I TCNGA 9 cut(s) 208, 241, 373, 412, 427, 547, 652, 700, 765
Hpy188III TCNNGA 4 cut(s) 57, 104, 569, 933
Hpy8I GTNNAC 3 cut(s) 680, 1004, 1186
Hpy99I CGWCG 1 cut(s) 765
HpyAV CCTTC 1 cut(s) 1019
HpyCH4III ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
HpyCH4V TGCA 8 cut(s) 311, 470, 521, 641, 827, 1004, 1036, 1162
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 988, 1037
HpyF3I CTNAG 5 cut(s) 199, 370, 651, 959, 1136
Hsp92II CATG 8 cut(s) 21, 107, 361, 388, 490, 818, 827, 1040
HspAI GCGC 1 cut(s) 1048
Kzo9I GATC 2 cut(s) 547, 743
LmnI GCTCC 4 cut(s) 398, 838, 1045, 1193
Lsp1109I GCAGC 2 cut(s) 639, 1174
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 790
MalI GATC 2 cut(s) 549, 745
MbiI CCGCTC 1 cut(s) 1196
MboI GATC 2 cut(s) 547, 743
MboII GAAGA 5 cut(s) 95, 120, 444, 534, 914
MhlI GDGCHC 2 cut(s) 395, 1006
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 3 cut(s) 86, 966, 1108
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 4 cut(s) 431, 548, 662, 1084
MmeI TCCRAC 3 cut(s) 100, 743, 1180
Mox20I TGGCCA 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 729
MscI TGGCCA 1 cut(s) 603
MseI TTAA 2 cut(s) 227, 1068
MslI CAYNNNNRTG 3 cut(s) 58, 465, 1170
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 1 cut(s) 795
MspR9I CCNGG 4 cut(s) 796, 988, 1128, 1190
MvaI CCWGG 3 cut(s) 988, 1128, 1190
MvnI CGCG 1 cut(s) 1048
MwoI GCNNNNNNNGC 3 cut(s) 22, 988, 1037
NciI CCSGG 1 cut(s) 796
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 466
NdeII GATC 2 cut(s) 547, 743
NlaIII CATG 8 cut(s) 21, 107, 361, 388, 490, 818, 827, 1040
NlaIV GGNNCC 3 cut(s) 6, 367, 394
NspI RCATGY 1 cut(s) 1040
PaeI GCATGC 1 cut(s) 1040
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 991
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 1 cut(s) 578
PflMI CCANNNNNTGG 2 cut(s) 385, 993
PkrI GCNGC 2 cut(s) 629, 1164
PleI GAGTC 4 cut(s) 430, 547, 661, 1083
PpsI GAGTC 4 cut(s) 430, 547, 661, 1083
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 3 cut(s) 986, 1126, 1188
PspFI CCCAGC 3 cut(s) 162, 215, 560
PspGI CCWGG 3 cut(s) 986, 1126, 1188
PspN4I GGNNCC 3 cut(s) 6, 367, 394
PspPI GGNCC 3 cut(s) 5, 254, 980
PspPPI RGGWCCY 1 cut(s) 254
PstNI CAGNNNCTG 2 cut(s) 206, 560
RsaI GTAC 3 cut(s) 95, 325, 446
RsaNI GTAC 3 cut(s) 94, 324, 445
RseI CAYNNNNRTG 3 cut(s) 58, 465, 1170
SalI GTCGAC 2 cut(s) 678, 1184
SaqAI TTAA 2 cut(s) 227, 1068
SatI GCNGC 2 cut(s) 628, 1163
Sau3AI GATC 2 cut(s) 547, 743
Sau96I GGNCC 3 cut(s) 5, 254, 980
SchI GAGTC 4 cut(s) 431, 548, 662, 1084
ScrFI CCNGG 4 cut(s) 796, 988, 1128, 1190
SduI GDGCHC 2 cut(s) 395, 1006
SfiI GGCCNNNNNGGCC 1 cut(s) 988
SinI GGWCC 2 cut(s) 5, 254
SmiMI CAYNNNNRTG 3 cut(s) 58, 465, 1170
SmlI CTYRAG 2 cut(s) 249, 931
SmoI CTYRAG 2 cut(s) 249, 931
SphI GCATGC 1 cut(s) 1040
Sse9I AATT 3 cut(s) 86, 966, 1108
SseBI AGGCCT 1 cut(s) 991
SsiI CCGC 4 cut(s) 607, 940, 1046, 1196
SspMI CTAG 1 cut(s) 36
StuI AGGCCT 1 cut(s) 991
StyD4I CCNGG 4 cut(s) 794, 986, 1126, 1188
StyI CCWWGG 4 cut(s) 357, 749, 953, 992
TaaI ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
TaqI TCGA 2 cut(s) 679, 1185
TasI AATT 3 cut(s) 86, 966, 1108
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 2 cut(s) 227, 1068
Tru9I TTAA 2 cut(s) 227, 1068
TscAI CASTG 4 cut(s) 295, 421, 439, 1177
TseI GCWGC 2 cut(s) 627, 1162
TspDTI ATGAA 6 cut(s) 92, 126, 225, 318, 475, 491
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 4 cut(s) 295, 421, 439, 1177
Van91I CCANNNNNTGG 2 cut(s) 385, 993
VneI GTGCAC 1 cut(s) 1002
VpaK11BI GGWCC 2 cut(s) 5, 254
XceI RCATGY 1 cut(s) 1040
XcmI CCANNNNNNNNNTGG 2 cut(s) 990, 1196
XmiI GTMKAC 2 cut(s) 679, 1185
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.