Rh4DG377000

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011785
Physical Location & Seq
Forward (+)
59451328 .. 59454227
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG377000.1

Sequence Viewer

Length: 1338 bp
ATGAGTGGCACTTGGCATAAGTCCCTAACCTGTGTCAGGAATGTTGATTTCATGCCACATGAGTCACATACGGCCAGTAGGGACTGGTCCGTTGGTCCAGACGAGCCTTGTTGGCGAACTAATACGAGCTTTTCTCCACCTCCTTCGAGATGGGATTTTCGATTCCAATCTGAAGAACTGCAATATGGTTTGCATGATGGTGTGCAACTATATGGGTCTTCTACTTCATCAAACAGTAGAGGAAGTAGGGGATGGGTGAGAGGGAATCATCTCTATAATCATCATTACTCTGCATCTGATGGTGCTGGGCTGTTTTTAAGTAGTTCGTCTGACCATTCTCAAGGTCCTCAGTGGACACCTCCAGCAATACAGGAAATCAGTATCGATGATTATGAAACTACAAAAAGGAGAGGTCCAGCTTTTGGGAAGTCATCCTTTAGACCTACCATGGAGGGAACCTCAGAAATTCAAGATACTGGGGGGTCCACGTCATCTCATTCAGACAGTAGTGAATCTGAACCCACGGTCAAGGCATCCCTATCTTGTCATCATACTTTCGCAAGTCGCCGTTCTTTCATGTCCAAACCTATATATCCCTTGTCATTTCCTCCACAAACTCCCCCCAGAGAGGCTTCTGACTTCACAGTTGCTGGGTTTCCTGAATTTGATGCTGCCACTCCTCAGAGGGATGGCCACCGCTGGAGCAGTGCCAGCAGCAGTGTTGATTTTACTGATGTTTCCGAGTCATTTGAGGCAGAAATTTCTGGTCGACACTTTAGTAATATGTCTGAGGGGTTTAGATGTGGCTTGTGTGATAGGTTCCTTTCACGGAGATCACCTTGGAGTTCTCGTCGCATAGTCAGAAGTGGAGATATGCCAATCACTGGGGTTCTTTCTTGTTGTCATGTTTTCCATGCAGAGTGCTTGGAGCAAACAACACCGAAGATCCGCAAAAGTGACCCTCCTTGCCCCCTATGTGCCCGACTTGAGGAGGAAAACCTCTCTGAACAGCAAGGCTTTTCTAGATTGCGAGCTAGTTTTCCAAGACTTAGACCAATTAGTGATGATGGACCATCCAGGCCTTGGGGATGTGCTCAGGTGGGAGATTGTGTTGAAGGGGCTTTGCATGCTCCCCCACGCAATAGTATGCTGTTGCTTAATAGGAGTCGTATAAAGAAAAATCTTTCTTTGAAAAGCAATTCGAGCAAAGAATTTCCTGGTAAATTGAGAAAAAGCGGCGCTTACTATTCACAACATCTGAGTGGAAAATCAGTTGATCAGGGAGCAGTTGGATGCTCAAAGTCAAAGATGACAGCAGGGCCGAGTATTAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

445

Amino Acids

49.16

Weight (kDa)

8.52

Isoelectric Point (pI)

64.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14180 AT1G14180
fragaria_vesca FvH4_4g31130 FvH4_4g31130
malus_domestica MD13G1070100.v1.1 MD16G1070500.v1.1
prunus_persica Prupe.1G276800_v2.0.a1
pyrus_communis pycom13g06280 pycom16g06130
rosa_chinensis RchiOBHm_Chr4g0439521
rosa_laevigata RLG00000006261
rosa_multiflora Rmu_sc0007276.1_g000009
rosa_roxburghii Rroxscaffold_5G00380440
rosa_rugosa Rorug04G0319300
rosa_samantha Rh4AG370400 Rh4BG382300 Rh4CG396900 Rh4DG377000
rosa_wichuraiana Rw4G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 422, 884, 1083
AccI GTMKAC 1 cut(s) 769
AciI CCGC 3 cut(s) 697, 949, 1236
AclWI GGATC 1 cut(s) 940
AcoI YGGCCR 2 cut(s) 72, 691
AcsI RAATTY 4 cut(s) 465, 662, 759, 1211
AcuI CTGAAG 1 cut(s) 192
AfiI CCNNNNNNNGG 6 cut(s) 36, 422, 628, 884, 988, 1083
AgsI TTSAA 3 cut(s) 470, 1115, 1192
AjiI CACGTC 1 cut(s) 489
AjnI CCWGG 2 cut(s) 1076, 1216
AluBI AGCT 4 cut(s) 129, 419, 1034, 1335
AluI AGCT 4 cut(s) 129, 419, 1034, 1335
Alw21I GWGCWC 1 cut(s) 1096
AlwI GGATC 1 cut(s) 940
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 4 cut(s) 72, 691, 1079, 1319
ApeKI GCWGC 2 cut(s) 671, 714
ApoI RAATTY 4 cut(s) 465, 662, 759, 1211
AspLEI GCGC 1 cut(s) 1241
AspS9I GGNCC 7 cut(s) 87, 95, 344, 413, 483, 1070, 1319
AsuHPI GGTGA 2 cut(s) 268, 828
AvaII GGWCC 6 cut(s) 87, 95, 344, 413, 483, 1070
BaeGI GKGCMC 1 cut(s) 982
BalI TGGCCA 1 cut(s) 693
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 1096
BbvI GCAGC 2 cut(s) 658, 726
BccI CCATC 7 cut(s) 144, 191, 246, 293, 683, 1061, 1081
BceAI ACGGC 2 cut(s) 87, 552
BciT130I CCWGG 2 cut(s) 1078, 1218
BclI TGATCA 1 cut(s) 1276
BfaI CTAG 2 cut(s) 1023, 1035
BfoI RGCGCY 1 cut(s) 1242
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 3 cut(s) 672, 715, 1237
BlsI GCNGC 3 cut(s) 673, 716, 1238
Bme1390I CCNGG 2 cut(s) 1078, 1218
Bme18I GGWCC 6 cut(s) 87, 95, 344, 413, 483, 1070
BmgBI CACGTC 1 cut(s) 489
BmgT120I GGNCC 7 cut(s) 87, 95, 344, 413, 483, 1070, 1319
BmiI GGNNCC 3 cut(s) 457, 484, 821
BmrFI CCNGG 2 cut(s) 1078, 1218
BmrI ACTGGG 2 cut(s) 486, 894
BmsI GCATC 4 cut(s) 302, 542, 658, 1283
BmuI ACTGGG 2 cut(s) 486, 894
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 4 cut(s) 118, 150, 443, 475
BpmI CTGGAG 2 cut(s) 345, 721
Bpu10I CCTNAGC 1 cut(s) 1095
BpuEI CTTGAG 2 cut(s) 324, 1007
Bsa29I ATCGAT 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 166
BsaJI CCNNGG 4 cut(s) 447, 522, 839, 1082
BsaXI ACNNNNNCTCC 4 cut(s) 835, 865, 1095, 1125
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 422, 628, 884, 988, 1083
Bse1I ACTGG 4 cut(s) 75, 89, 481, 889
Bse8I GATNNNNATC 1 cut(s) 166
BseBI CCWGG 2 cut(s) 1078, 1218
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 4 cut(s) 447, 522, 839, 1082
BseGI GGATG 7 cut(s) 257, 431, 533, 694, 1073, 1094, 1298
BseJI GATNNNNATC 1 cut(s) 166
BseLI CCNNNNNNNGG 6 cut(s) 36, 422, 628, 884, 988, 1083
BseMII CTCAG 6 cut(s) 362, 474, 695, 780, 1109, 1250
BseNI ACTGG 4 cut(s) 75, 89, 481, 889
BseRI GAGGAG 2 cut(s) 669, 1004
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 2 cut(s) 658, 726
BseYI CCCAGC 2 cut(s) 305, 650
BshFI GGCC 4 cut(s) 74, 693, 1081, 1321
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 1096
BslFI GGGAC 2 cut(s) 7, 95
BslI CCNNNNNNNGG 6 cut(s) 36, 422, 628, 884, 988, 1083
BsmFI GGGAC 2 cut(s) 7, 95
BsnI GGCC 4 cut(s) 74, 693, 1081, 1321
Bsp1286I GDGCHC 2 cut(s) 982, 1096
Bsp143I GATC 3 cut(s) 833, 945, 1276
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 3 cut(s) 697, 949, 1236
BspANI GGCC 4 cut(s) 74, 693, 1081, 1321
BspCNI CTCAG 6 cut(s) 361, 473, 694, 781, 1108, 1251
BspDI ATCGAT 1 cut(s) 384
BspLI GGNNCC 3 cut(s) 457, 484, 821
BspPI GGATC 1 cut(s) 940
BsrI ACTGG 4 cut(s) 75, 89, 481, 889
BssECI CCNNGG 4 cut(s) 447, 522, 839, 1082
BssMI GATC 3 cut(s) 833, 945, 1276
BssT1I CCWWGG 3 cut(s) 447, 839, 1082
Bst2UI CCWGG 2 cut(s) 1078, 1218
Bst4CI ACNGT 4 cut(s) 236, 506, 526, 646
BstC8I GCNNGC 3 cut(s) 712, 1032, 1128
BstDEI CTNAG 7 cut(s) 348, 460, 681, 789, 1049, 1095, 1259
BstDSI CCRYGG 2 cut(s) 447, 522
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 7 cut(s) 257, 431, 533, 694, 1073, 1094, 1298
BstH2I RGCGCY 1 cut(s) 1242
BstHHI GCGC 1 cut(s) 1241
BstKTI GATC 3 cut(s) 836, 948, 1279
BstMBI GATC 3 cut(s) 833, 945, 1276
BstMWI GCNNNNNNNGC 4 cut(s) 112, 711, 1127, 1203
BstNI CCWGG 2 cut(s) 1078, 1218
BstNSI RCATGY 1 cut(s) 1130
BstSCI CCNGG 2 cut(s) 1076, 1216
BstSLI GKGCMC 1 cut(s) 982
BstV1I GCAGC 2 cut(s) 658, 726
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 945
BstYI RGATCY 1 cut(s) 945
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 4 cut(s) 74, 693, 1081, 1321
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 2 cut(s) 447, 522
BtrI CACGTC 1 cut(s) 489
BtsCI GGATG 7 cut(s) 257, 431, 533, 694, 1073, 1094, 1298
BtsI GCAGTG 2 cut(s) 712, 724
BtsIMutI CAGTG 4 cut(s) 356, 712, 724, 882
Cac8I GCNNGC 3 cut(s) 712, 1032, 1128
CaiI CAGNNNCTG 1 cut(s) 650
CfoI GCGC 1 cut(s) 1241
Cfr13I GGNCC 7 cut(s) 87, 95, 344, 413, 483, 1070, 1319
ClaI ATCGAT 1 cut(s) 384
CviAII CATG 8 cut(s) 52, 59, 194, 448, 577, 905, 914, 1127
DdeI CTNAG 7 cut(s) 348, 460, 681, 789, 1049, 1095, 1259
DpnI GATC 3 cut(s) 835, 947, 1278
DpnII GATC 3 cut(s) 833, 945, 1276
EaeI YGGCCR 2 cut(s) 72, 691
Eco130I CCWWGG 3 cut(s) 447, 839, 1082
Eco147I AGGCCT 1 cut(s) 1081
Eco47I GGWCC 6 cut(s) 87, 95, 344, 413, 483, 1070
Eco57I CTGAAG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 1076, 1216
EcoT14I CCWWGG 3 cut(s) 447, 839, 1082
ErhI CCWWGG 3 cut(s) 447, 839, 1082
FaeI CATG 8 cut(s) 55, 62, 197, 451, 580, 908, 917, 1130
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 1225, 1257
FaqI GGGAC 2 cut(s) 7, 95
FatI CATG 8 cut(s) 51, 58, 193, 447, 576, 904, 913, 1126
FbaI TGATCA 1 cut(s) 1276
FblI GTMKAC 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 672, 715, 1237
FokI GGATG 7 cut(s) 264, 418, 520, 701, 1060, 1101, 1305
Fsp4HI GCNGC 3 cut(s) 672, 715, 1237
FspBI CTAG 2 cut(s) 1023, 1035
GlaI GCGC 1 cut(s) 1240
GluI GCNGC 3 cut(s) 672, 715, 1237
GsaI CCCAGC 2 cut(s) 309, 654
GsuI CTGGAG 2 cut(s) 345, 721
HaeII RGCGCY 1 cut(s) 1242
HaeIII GGCC 4 cut(s) 74, 693, 1081, 1321
HhaI GCGC 1 cut(s) 1241
Hin1II CATG 8 cut(s) 55, 62, 197, 451, 580, 908, 917, 1130
Hin6I GCGC 1 cut(s) 1239
HinP1I GCGC 1 cut(s) 1239
HincII GTYRAC 1 cut(s) 770
HindII GTYRAC 1 cut(s) 770
HinfI GANTC 6 cut(s) 62, 162, 265, 512, 743, 1165
HphI GGTGA 2 cut(s) 268, 828
Hpy166II GTNNAC 3 cut(s) 354, 486, 770
Hpy188III TCNNGA 6 cut(s) 37, 98, 147, 470, 659, 1023
Hpy8I GTNNAC 3 cut(s) 354, 486, 770
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 2 cut(s) 153, 1109
HpyCH4III ACNGT 4 cut(s) 236, 506, 526, 646
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 6 cut(s) 181, 193, 205, 293, 917, 1126
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 711, 1127, 1203
HpyF3I CTNAG 7 cut(s) 348, 460, 681, 789, 1049, 1095, 1259
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 8 cut(s) 55, 62, 197, 451, 580, 908, 917, 1130
HspAI GCGC 1 cut(s) 1239
Ksp22I TGATCA 1 cut(s) 1276
Kzo9I GATC 3 cut(s) 833, 945, 1276
LmnI GCTCC 4 cut(s) 702, 928, 1135, 1283
Lsp1109I GCAGC 2 cut(s) 658, 726
LweI GCATC 4 cut(s) 302, 542, 658, 1283
MaeI CTAG 2 cut(s) 1023, 1035
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 63, 956
MalI GATC 3 cut(s) 835, 947, 1278
MboI GATC 3 cut(s) 833, 945, 1276
MboII GAAGA 3 cut(s) 185, 210, 955
MflI RGATCY 1 cut(s) 945
MhlI GDGCHC 2 cut(s) 982, 1096
MlsI TGGCCA 1 cut(s) 693
MluCI AATT 7 cut(s) 465, 662, 759, 1056, 1198, 1211, 1223
MluNI TGGCCA 1 cut(s) 693
MlyI GAGTC 3 cut(s) 71, 752, 1174
MmeI TCCRAC 1 cut(s) 1270
Mox20I TGGCCA 1 cut(s) 693
MscI TGGCCA 1 cut(s) 693
MseI TTAA 3 cut(s) 317, 1158, 1329
MslI CAYNNNNRTG 2 cut(s) 198, 1260
Msp20I TGGCCA 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 699
MspR9I CCNGG 2 cut(s) 1078, 1218
MvaI CCWGG 2 cut(s) 1078, 1218
MwoI GCNNNNNNNGC 4 cut(s) 112, 711, 1127, 1203
NcoI CCATGG 1 cut(s) 447
NdeII GATC 3 cut(s) 833, 945, 1276
NlaIII CATG 8 cut(s) 55, 62, 197, 451, 580, 908, 917, 1130
NlaIV GGNNCC 3 cut(s) 457, 484, 821
NmuCI GTSAC 2 cut(s) 63, 956
NspI RCATGY 1 cut(s) 1130
PaeI GCATGC 1 cut(s) 1130
PceI AGGCCT 1 cut(s) 1081
PfeI GAWTC 3 cut(s) 162, 265, 512
PflMI CCANNNNNTGG 3 cut(s) 422, 884, 1083
PkrI GCNGC 3 cut(s) 673, 716, 1238
PleI GAGTC 3 cut(s) 70, 751, 1173
PpsI GAGTC 3 cut(s) 70, 751, 1173
PpuMI RGGWCCY 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 1076, 1216
PspFI CCCAGC 2 cut(s) 305, 650
PspGI CCWGG 2 cut(s) 1076, 1216
PspN4I GGNNCC 3 cut(s) 457, 484, 821
PspPI GGNCC 7 cut(s) 87, 95, 344, 413, 483, 1070, 1319
PspPPI RGGWCCY 1 cut(s) 344
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 945
RseI CAYNNNNRTG 2 cut(s) 198, 1260
SalI GTCGAC 1 cut(s) 768
SaqAI TTAA 3 cut(s) 317, 1158, 1329
SatI GCNGC 3 cut(s) 672, 715, 1237
Sau3AI GATC 3 cut(s) 833, 945, 1276
Sau96I GGNCC 7 cut(s) 87, 95, 344, 413, 483, 1070, 1319
SchI GAGTC 3 cut(s) 71, 752, 1174
ScrFI CCNGG 2 cut(s) 1078, 1218
SduI GDGCHC 2 cut(s) 982, 1096
SfaNI GCATC 4 cut(s) 302, 542, 658, 1283
SinI GGWCC 6 cut(s) 87, 95, 344, 413, 483, 1070
SmiMI CAYNNNNRTG 2 cut(s) 198, 1260
SmlI CTYRAG 2 cut(s) 339, 986
SmoI CTYRAG 2 cut(s) 339, 986
SphI GCATGC 1 cut(s) 1130
Sse9I AATT 7 cut(s) 465, 662, 759, 1056, 1198, 1211, 1223
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 3 cut(s) 697, 949, 1236
SspMI CTAG 2 cut(s) 1023, 1035
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 2 cut(s) 1076, 1216
StyI CCWWGG 3 cut(s) 447, 839, 1082
TaaI ACNGT 4 cut(s) 236, 506, 526, 646
TaiI ACGT 1 cut(s) 491
TaqI TCGA 5 cut(s) 146, 160, 384, 769, 1202
TasI AATT 7 cut(s) 465, 662, 759, 1056, 1198, 1211, 1223
TauI GCSGC 1 cut(s) 1239
TfiI GAWTC 3 cut(s) 162, 265, 512
Tru1I TTAA 3 cut(s) 317, 1158, 1329
Tru9I TTAA 3 cut(s) 317, 1158, 1329
TscAI CASTG 4 cut(s) 356, 712, 724, 889
TseFI GTSAC 2 cut(s) 63, 956
TseI GCWGC 2 cut(s) 671, 714
Tsp45I GTSAC 2 cut(s) 63, 956
TspDTI ATGAA 4 cut(s) 40, 216, 408, 565
TspGWI ACGGA 2 cut(s) 79, 844
TspRI CASTG 4 cut(s) 356, 712, 724, 889
Van91I CCANNNNNTGG 3 cut(s) 422, 884, 1083
VpaK11BI GGWCC 6 cut(s) 87, 95, 344, 413, 483, 1070
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 4 cut(s) 465, 662, 759, 1211
XbaI TCTAGA 1 cut(s) 1022
XceI RCATGY 1 cut(s) 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 1080
XmiI GTMKAC 1 cut(s) 769
XspI CTAG 2 cut(s) 1023, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.