MD16G1070500.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011785
Physical Location & Seq
Forward (+)
4937701 .. 4940546
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1070500.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGGTCCAGAAGAGCCATGCTGGAGAACTAATACGAGCTTCTCACCACCCCCATCAAGATGGGATTTCCGTTTCCAACCTGAAGAATTACAGTACGGTTTTCATGATGGTAATCAGCAATATGGGTCTTCTACTTCATCAAACAGTAAAGGAAGTAGGGGCTGGGTGAGAGGCAACAATCTCTATAATCATCACTACTCGGTGTCTGATGGTGCTGGGCTGTTTTTAAGTAGTCCATCTGACCTTTCTCAAGGTCCTCAACGGACACCTCCAGCAATACAGGAAATCAGTGTAGATGATTATGAAACTGCAAAAAAGAGAGGTCAAGCTTTGGGGCATACATCCATTAGACCTACCATGGAGGGAACCTCAGAAATTCAAGAAATGGGGGATTCCACCTCGTCCCGTTCAGACGGTAGCGAGTCTGAACCCACAGTGAAGCCGTACCTATCTTCCCATCATACATATCCAAGTCGGCGTTCGTTCATGGCCAAACCTATTCATCCCTTGTCCTTTCCTGCACAAACACCGCCCAGAGAGGCTTCTGATCTCACAGTTACTGGGTTTCTGGAGTATGATTCTGCCACTCCTCAAAGAGATGGCCACCGCTGGAGTAGTGCCAGTAGCAGCATTGATTTTGCAGATGTTTCTGAGTCATTTGAGGCAGAGATTTCTGGTCAACCTTGTAATAATGTGAATGTGTCTGATGGTTTTAGATGTGGTTTGTGTGAAAGGTTTCTTTCACAGAGATCGCCTTGGAGTTCTCGTCGGATAGTGAGAAGTGGAGATATGCCAGTTACCGGTGTTCTTTCTTGCTCTCATGTTTTCCATGCAGAGTGCTTGGAGCAAACAACACCCAAGACACGAAAAAGCGACCCTCCCTGTCCCCTATGTGCTAGACTTGAGGAGGAAAATCTCCAAGACCAGCAAGGCTTCTCAAGATTGCGGGCTGGTTTTCCAAGGCATAGACCAATAAGTGATGATGGATCATCCAGGCCTTGGGGTTGTGCACAGGTGGGAGATTGCGTTGAAGGGGCTTTGCATGCTCCTCCGCGCAATAGTATGCTGATGCTTAACAGGAGTCGCATAAAAAAAAACCTTTCTTTGAAAGGTAATTTGGGCAAAGAGTTTCCAGGTAAGCTAAGAAAAAGTGGACCTCACTCTTTACAGCATTTGAGTGGAAAGTCAGTTGATCAGGGAATGGTTGGAAGCTCAAAATCGAAGATGACAGCAGGCCCGAGTTTTAAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

45.89

Weight (kDa)

8.29

Isoelectric Point (pI)

59.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14180 AT1G14180
fragaria_vesca FvH4_4g31130 FvH4_4g31130
malus_domestica MD13G1070100.v1.1 MD16G1070500.v1.1
prunus_persica Prupe.1G276800_v2.0.a1
pyrus_communis pycom13g06280 pycom16g06130
rosa_chinensis RchiOBHm_Chr4g0439521
rosa_laevigata RLG00000006261
rosa_multiflora Rmu_sc0007276.1_g000009
rosa_roxburghii Rroxscaffold_5G00380440
rosa_rugosa Rorug04G0319300
rosa_samantha Rh4AG370400 Rh4BG382300 Rh4CG396900 Rh4DG377000
rosa_wichuraiana Rw4G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 999
AccII CGCG 1 cut(s) 1054
AciI CCGC 4 cut(s) 530, 607, 946, 1052
AclWI GGATC 1 cut(s) 994
AcoI YGGCCR 2 cut(s) 489, 601
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 2 cut(s) 95, 446
AfiI CCNNNNNNNGG 2 cut(s) 800, 999
AgeI ACCGGT 1 cut(s) 800
AgsI TTSAA 3 cut(s) 380, 1031, 1108
AjnI CCWGG 2 cut(s) 992, 1132
AjuI GAANNNNNNNTTGG 4 cut(s) 463, 495, 1100, 1132
AluBI AGCT 4 cut(s) 39, 329, 1141, 1212
AluI AGCT 4 cut(s) 39, 329, 1141, 1212
Alw21I GWGCWC 1 cut(s) 1012
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 1 cut(s) 994
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 1237
AoxI GGCC 4 cut(s) 489, 601, 995, 1234
ApaLI GTGCAC 1 cut(s) 1008
ApeKI GCWGC 1 cut(s) 627
ApoI RAATTY 1 cut(s) 375
AsiGI ACCGGT 1 cut(s) 800
Asp700I GAANNNNTTC 1 cut(s) 735
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 4 cut(s) 5, 254, 1154, 1235
AsuHPI GGTGA 2 cut(s) 36, 178
AvaI CYCGRG 1 cut(s) 1237
AvaII GGWCC 3 cut(s) 5, 254, 1154
BaeGI GKGCMC 1 cut(s) 1012
BalI TGGCCA 2 cut(s) 491, 603
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 1012
BbvI GCAGC 1 cut(s) 639
BccI CCATC 9 cut(s) 54, 61, 101, 203, 244, 465, 593, 701, 977
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 994, 1134
BclI TGATCA 1 cut(s) 1192
BfaI CTAG 1 cut(s) 897
BisI GCNGC 1 cut(s) 628
BlsI GCNGC 1 cut(s) 629
Bme1390I CCNGG 2 cut(s) 994, 1134
Bme18I GGWCC 3 cut(s) 5, 254, 1154
BmeT110I CYCGRG 1 cut(s) 1237
BmgT120I GGNCC 4 cut(s) 5, 254, 1154, 1235
BmiI GGNNCC 2 cut(s) 6, 367
BmrFI CCNGG 2 cut(s) 994, 1134
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 1059
BmuI ACTGGG 1 cut(s) 570
BpiI GAAGAC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 4 cut(s) 43, 255, 590, 631
BpuEI CTTGAG 3 cut(s) 234, 922, 923
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 4 cut(s) 357, 755, 959, 998
BsaWI WCCGGW 1 cut(s) 800
BsaXI ACNNNNNCTCC 4 cut(s) 751, 781, 1011, 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 800, 999
Bse118I RCCGGY 1 cut(s) 800
Bse1I ACTGG 3 cut(s) 565, 621, 794
Bse8I GATNNNNATC 1 cut(s) 111
BseBI CCWGG 2 cut(s) 994, 1134
BseDI CCNNGG 4 cut(s) 357, 755, 959, 998
BseGI GGATG 3 cut(s) 341, 502, 989
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 800, 999
BseMII CTCAG 2 cut(s) 384, 642
BseNI ACTGG 3 cut(s) 565, 621, 794
BseRI GAGGAG 3 cut(s) 579, 920, 1038
BseSI GKGCMC 1 cut(s) 1012
BseXI GCAGC 1 cut(s) 639
BseYI CCCAGC 2 cut(s) 162, 215
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 1054
BshFI GGCC 4 cut(s) 491, 603, 997, 1236
BshTI ACCGGT 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 1012
BsiHKCI CYCGRG 1 cut(s) 1237
BsiSI CCGG 1 cut(s) 801
BslFI GGGAC 2 cut(s) 388, 870
BslI CCNNNNNNNGG 2 cut(s) 800, 999
BsmFI GGGAC 2 cut(s) 388, 870
BsnI GGCC 4 cut(s) 491, 603, 997, 1236
BsoBI CYCGRG 1 cut(s) 1237
Bsp1286I GDGCHC 1 cut(s) 1012
Bsp143I GATC 4 cut(s) 547, 749, 986, 1192
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 4 cut(s) 530, 607, 946, 1052
BspANI GGCC 4 cut(s) 491, 603, 997, 1236
BspCNI CTCAG 2 cut(s) 383, 643
BspFNI CGCG 1 cut(s) 1054
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 6, 367
BspPI GGATC 1 cut(s) 994
BspQI GCTCTTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 800
BsrI ACTGG 3 cut(s) 565, 621, 794
BssAI RCCGGY 1 cut(s) 800
BssECI CCNNGG 4 cut(s) 357, 755, 959, 998
BssMI GATC 4 cut(s) 547, 749, 986, 1192
BssT1I CCWWGG 4 cut(s) 357, 755, 959, 998
Bst2UI CCWGG 2 cut(s) 994, 1134
Bst4CI ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
Bst6I CTCTTC 1 cut(s) 6
BstC8I GCNNGC 3 cut(s) 948, 1044, 1234
BstDEI CTNAG 3 cut(s) 370, 651, 1142
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 341, 502, 989
BstFNI CGCG 1 cut(s) 1054
BstHHI GCGC 1 cut(s) 1056
BstKTI GATC 4 cut(s) 550, 752, 989, 1195
BstMBI GATC 4 cut(s) 547, 749, 986, 1192
BstMWI GCNNNNNNNGC 1 cut(s) 1043
BstNI CCWGG 2 cut(s) 994, 1134
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 2 cut(s) 992, 1132
BstSLI GKGCMC 1 cut(s) 1012
BstUI CGCG 1 cut(s) 1054
BstV1I GCAGC 1 cut(s) 639
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 60
BsuRI GGCC 4 cut(s) 491, 603, 997, 1236
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 341, 502, 989
BtsIMutI CAGTG 2 cut(s) 295, 441
Cac8I GCNNGC 3 cut(s) 948, 1044, 1234
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 1056
Cfr10I RCCGGY 1 cut(s) 800
Cfr13I GGNCC 4 cut(s) 5, 254, 1154, 1235
Csp6I GTAC 2 cut(s) 94, 445
CspAI ACCGGT 1 cut(s) 800
CviAII CATG 7 cut(s) 18, 104, 358, 487, 821, 830, 1043
CviQI GTAC 2 cut(s) 94, 445
DdeI CTNAG 3 cut(s) 370, 651, 1142
DpnI GATC 4 cut(s) 549, 751, 988, 1194
DpnII GATC 4 cut(s) 547, 749, 986, 1192
DraI TTTAAA 1 cut(s) 1246
EaeI YGGCCR 2 cut(s) 489, 601
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Eco130I CCWWGG 4 cut(s) 357, 755, 959, 998
Eco147I AGGCCT 1 cut(s) 997
Eco47I GGWCC 3 cut(s) 5, 254, 1154
Eco57I CTGAAG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 1237
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 992, 1132
EcoT14I CCWWGG 4 cut(s) 357, 755, 959, 998
ErhI CCWWGG 4 cut(s) 357, 755, 959, 998
FaeI CATG 7 cut(s) 21, 107, 361, 490, 824, 833, 1046
FaqI GGGAC 2 cut(s) 388, 870
FatI CATG 7 cut(s) 17, 103, 357, 486, 820, 829, 1042
FauI CCCGC 1 cut(s) 939
FbaI TGATCA 1 cut(s) 1192
Fnu4HI GCNGC 1 cut(s) 628
FokI GGATG 3 cut(s) 328, 489, 976
Fsp4HI GCNGC 1 cut(s) 628
FspBI CTAG 1 cut(s) 897
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 1 cut(s) 628
GsaI CCCAGC 2 cut(s) 166, 219
GsuI CTGGAG 4 cut(s) 43, 255, 590, 631
HaeIII GGCC 4 cut(s) 491, 603, 997, 1236
HapII CCGG 1 cut(s) 801
HhaI GCGC 1 cut(s) 1056
Hin1II CATG 7 cut(s) 21, 107, 361, 490, 824, 833, 1046
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HincII GTYRAC 1 cut(s) 680
HindII GTYRAC 1 cut(s) 680
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 5 cut(s) 392, 422, 578, 653, 1081
HpaII CCGG 1 cut(s) 801
HphI GGTGA 2 cut(s) 36, 178
Hpy166II GTNNAC 3 cut(s) 680, 1010, 1154
Hpy188I TCNGA 9 cut(s) 208, 241, 373, 412, 427, 547, 652, 706, 771
Hpy188III TCNNGA 6 cut(s) 8, 57, 104, 380, 569, 939
Hpy8I GTNNAC 3 cut(s) 680, 1010, 1154
Hpy99I CGWCG 1 cut(s) 771
HpyAV CCTTC 1 cut(s) 1025
HpyCH4III ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
HpyCH4V TGCA 6 cut(s) 311, 521, 641, 833, 1010, 1042
HpyF10VI GCNNNNNNNGC 1 cut(s) 1043
HpyF3I CTNAG 3 cut(s) 370, 651, 1142
Hsp92II CATG 7 cut(s) 21, 107, 361, 490, 824, 833, 1046
HspAI GCGC 1 cut(s) 1054
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 4 cut(s) 547, 749, 986, 1192
LguI GCTCTTC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 844, 1051
Lsp1109I GCAGC 1 cut(s) 639
LweI GCATC 1 cut(s) 1059
MaeI CTAG 1 cut(s) 897
MaeIII GTNAC 2 cut(s) 556, 796
MalI GATC 4 cut(s) 549, 751, 988, 1194
MboI GATC 4 cut(s) 547, 749, 986, 1192
MboII GAAGA 5 cut(s) 23, 95, 120, 444, 1234
MhlI GDGCHC 1 cut(s) 1012
MlsI TGGCCA 2 cut(s) 491, 603
MluCI AATT 3 cut(s) 86, 375, 1114
MluNI TGGCCA 2 cut(s) 491, 603
MlyI GAGTC 3 cut(s) 431, 662, 1090
MmeI TCCRAC 3 cut(s) 100, 749, 1186
Mox20I TGGCCA 2 cut(s) 491, 603
MroXI GAANNNNTTC 1 cut(s) 735
MscI TGGCCA 2 cut(s) 491, 603
MseI TTAA 3 cut(s) 227, 1074, 1245
MslI CAYNNNNRTG 2 cut(s) 58, 1176
Msp20I TGGCCA 2 cut(s) 491, 603
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 1 cut(s) 801
MspR9I CCNGG 2 cut(s) 994, 1134
MvaI CCWGG 2 cut(s) 994, 1134
MvnI CGCG 1 cut(s) 1054
MwoI GCNNNNNNNGC 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 357
NdeII GATC 4 cut(s) 547, 749, 986, 1192
NlaIII CATG 7 cut(s) 21, 107, 361, 490, 824, 833, 1046
NlaIV GGNNCC 2 cut(s) 6, 367
NspI RCATGY 1 cut(s) 1046
PaeI GCATGC 1 cut(s) 1046
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 997
PciSI GCTCTTC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 2 cut(s) 392, 578
PflMI CCANNNNNTGG 1 cut(s) 999
PinAI ACCGGT 1 cut(s) 800
PkrI GCNGC 1 cut(s) 629
PleI GAGTC 3 cut(s) 430, 661, 1089
PpsI GAGTC 3 cut(s) 430, 661, 1089
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 992, 1132
PspFI CCCAGC 2 cut(s) 162, 215
PspGI CCWGG 2 cut(s) 992, 1132
PspN4I GGNNCC 2 cut(s) 6, 367
PspPI GGNCC 4 cut(s) 5, 254, 1154, 1235
PspPPI RGGWCCY 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 95, 446
RsaNI GTAC 2 cut(s) 94, 445
RseI CAYNNNNRTG 2 cut(s) 58, 1176
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 3 cut(s) 227, 1074, 1245
SatI GCNGC 1 cut(s) 628
Sau3AI GATC 4 cut(s) 547, 749, 986, 1192
Sau96I GGNCC 4 cut(s) 5, 254, 1154, 1235
SchI GAGTC 3 cut(s) 431, 662, 1090
ScrFI CCNGG 2 cut(s) 994, 1134
SduI GDGCHC 1 cut(s) 1012
SfaNI GCATC 1 cut(s) 1059
SinI GGWCC 3 cut(s) 5, 254, 1154
SmiMI CAYNNNNRTG 2 cut(s) 58, 1176
SmlI CTYRAG 3 cut(s) 249, 902, 937
SmoI CTYRAG 3 cut(s) 249, 902, 937
SphI GCATGC 1 cut(s) 1046
Sse9I AATT 3 cut(s) 86, 375, 1114
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 4 cut(s) 530, 607, 946, 1052
SspMI CTAG 1 cut(s) 897
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 2 cut(s) 992, 1132
StyI CCWWGG 4 cut(s) 357, 755, 959, 998
TaaI ACNGT 6 cut(s) 93, 98, 146, 416, 436, 556
TaqI TCGA 1 cut(s) 1220
TasI AATT 3 cut(s) 86, 375, 1114
TfiI GAWTC 2 cut(s) 392, 578
Tru1I TTAA 3 cut(s) 227, 1074, 1245
Tru9I TTAA 3 cut(s) 227, 1074, 1245
TscAI CASTG 2 cut(s) 295, 441
TseI GCWGC 1 cut(s) 627
TspDTI ATGAA 5 cut(s) 92, 126, 318, 475, 491
TspGWI ACGGA 2 cut(s) 59, 277
TspRI CASTG 2 cut(s) 295, 441
Van91I CCANNNNNTGG 1 cut(s) 999
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 3 cut(s) 5, 254, 1154
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 1046
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.