pycom13g06280

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011785
Physical Location & Seq
Forward (+)
4062669 .. 4065058
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06280.2

Sequence Viewer

Length: 1251 bp
ATGGGTCCAGCAGAGCCATGCTGGCGAACTAATACCAGCTTCTCACCACCCCCATCAAGATGGGATTTCCGTTTCCAACCTGAAGAATTACAATATGGTTTTCATGATGGTATTCAGCAATATGGGTCTTCTACTTCATCAAACAGTAAAGGAAGTAGGGGCTGGGTAAGAGGCAACAATCTCTACAATCACCACTACTCAGCTTCTGATGGTGCTGGGCTGTTTTTAAGTGGTTCATCTGACCTTTCTCAAGGGCCTCAATGGACACCTCCAGCAATACAGGAAATCAGTGTAGATGATTATGAAACTGCAAAAAGGAGAGGTACAGCTTTGGGGCATACATCCTTTAGACCTACCATGGAGGGAACCTCAGAAATCCAAGACATGGGGGGCTCCACCTCATCCCGTTCGGACAGTGGCGAGTCTGAACCCACTGTCAAGCCGTACCTATCTTCTCATCATACATATGCAAGTCGGTGTTCGTTCATGTCCAAACCTATTCATCCCTTGTCCTTTCCTGCACAAACTTCCCCCAGAGAGTCTTCTGATCGATCACAGTTGCTGGGTTTCCAGAGTATGATTCTGCTACTCCTCAAAGAGATGGCCACCGCTGGAGTAGTGCCAGTAGCAGCATTGATTTTGCAGATGTTTCTGAGTCATTTGAGGCAGAGGTTTCTGGTCGACCTTGTAATAATGTATCTGATGGTTTTAGATGTGGCATGTGTGAAAGGTTTCTTTCACAGAGATCGACCTTGGAGTTCTCGTCGGATAGTGAGAAGTGGAGATATGCCAGTTACCGGGGTTCTTTCTTGCTCTCATGTTTTCCATGCAGAGTGCTTGGAGCAAACAACACCCAAGACACGAAAAAGCGACCCTCCCTGTCCCCTATGTGCCAGATTTGAGGAAGAAAATCTCCAAGAGCAACAAGGCTTCTCAAGATTGCGGGCTGGTTTTCCAAGGCTTAGACCAATTAGTGATGATGGGCCATCCAGGCCTTGGGGCTGTGCACAGGTGGGAGATTGCGTTGAAGGGGCTTTGCATGCTCCCCCGCGCAATAGTATGCTGTTGCTTAACAGGAGTCGCATAAAGAAAAATCTTTCTTTGAAAGGTAATTTGGGCAAAGAGTTTCCAGGTAAGCTAAGAAAAATTGGTTCTCACTCTTTGCAGCATTTGAGTGGAAAATCAGTCGACCAGGGAGCAGTTGGAAGCTCAAAATCAAAGATGACAGCAGGCCCGAGTTTTAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

417

Amino Acids

45.89

Weight (kDa)

9.27

Isoelectric Point (pI)

56.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14180 AT1G14180
fragaria_vesca FvH4_4g31130 FvH4_4g31130
malus_domestica MD13G1070100.v1.1 MD16G1070500.v1.1
prunus_persica Prupe.1G276800_v2.0.a1
pyrus_communis pycom13g06280 pycom16g06130
rosa_chinensis RchiOBHm_Chr4g0439521
rosa_laevigata RLG00000006261
rosa_multiflora Rmu_sc0007276.1_g000009
rosa_roxburghii Rroxscaffold_5G00380440
rosa_rugosa Rorug04G0319300
rosa_samantha Rh4AG370400 Rh4BG382300 Rh4CG396900 Rh4DG377000
rosa_wichuraiana Rw4G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 385, 996
AccI GTMKAC 2 cut(s) 681, 1188
AccII CGCG 1 cut(s) 1051
AciI CCGC 3 cut(s) 609, 943, 1049
AcoI YGGCCR 1 cut(s) 603
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 2 cut(s) 325, 446
AfiI CCNNNNNNNGG 3 cut(s) 385, 797, 996
AgsI TTSAA 2 cut(s) 1028, 1105
AjnI CCWGG 3 cut(s) 989, 1129, 1191
AjuI GAANNNNNNNTTGG 2 cut(s) 1097, 1129
AluBI AGCT 5 cut(s) 39, 203, 329, 1138, 1209
AluI AGCT 5 cut(s) 39, 203, 329, 1138, 1209
Alw21I GWGCWC 1 cut(s) 1009
Alw44I GTGCAC 1 cut(s) 1005
AlwNI CAGNNNCTG 2 cut(s) 206, 562
Ama87I CYCGRG 1 cut(s) 1234
AoxI GGCC 5 cut(s) 254, 603, 983, 992, 1231
ApaLI GTGCAC 1 cut(s) 1005
ApeKI GCWGC 2 cut(s) 629, 1165
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 1053
AspS9I GGNCC 4 cut(s) 5, 254, 983, 1232
AsuC2I CCSGG 1 cut(s) 799
AsuHPI GGTGA 2 cut(s) 36, 182
AvaI CYCGRG 1 cut(s) 1234
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 1009
BalI TGGCCA 1 cut(s) 605
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 2 cut(s) 120, 534
Bbv12I GWGCWC 1 cut(s) 1009
BbvI GCAGC 2 cut(s) 641, 1177
BccI CCATC 8 cut(s) 54, 61, 101, 203, 595, 697, 974, 994
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 3 cut(s) 991, 1131, 1193
BcnI CCSGG 1 cut(s) 799
BglI GCCNNNNNGGC 2 cut(s) 22, 991
BisI GCNGC 2 cut(s) 630, 1166
BlsI GCNGC 2 cut(s) 631, 1167
Bme1390I CCNGG 4 cut(s) 799, 991, 1131, 1193
Bme18I GGWCC 1 cut(s) 5
BmeT110I CYCGRG 1 cut(s) 1234
BmgT120I GGNCC 4 cut(s) 5, 254, 983, 1232
BmiI GGNNCC 3 cut(s) 6, 367, 394
BmrFI CCNGG 4 cut(s) 799, 991, 1131, 1193
BpiI GAAGAC 2 cut(s) 120, 534
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 2 cut(s) 255, 633
BpuEI CTTGAG 2 cut(s) 234, 919
BpuMI CCSGG 1 cut(s) 799
Bsa29I ATCGAT 1 cut(s) 550
BsaJI CCNNGG 6 cut(s) 357, 752, 798, 956, 995, 1192
BsaXI ACNNNNNCTCC 4 cut(s) 748, 778, 1008, 1038
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 797, 996
Bse1I ACTGG 2 cut(s) 623, 791
BseBI CCWGG 3 cut(s) 991, 1131, 1193
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 6 cut(s) 357, 752, 798, 956, 995, 1192
BseGI GGATG 4 cut(s) 341, 401, 502, 986
BseLI CCNNNNNNNGG 3 cut(s) 385, 797, 996
BseMII CTCAG 3 cut(s) 213, 384, 644
BseNI ACTGG 2 cut(s) 623, 791
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 1009
BseXI GCAGC 2 cut(s) 641, 1177
BseYI CCCAGC 3 cut(s) 162, 215, 562
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 1051
BshFI GGCC 5 cut(s) 256, 605, 985, 994, 1233
BshVI ATCGAT 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 1009
BsiHKCI CYCGRG 1 cut(s) 1234
BsiSI CCGG 1 cut(s) 798
BslFI GGGAC 1 cut(s) 867
BslI CCNNNNNNNGG 3 cut(s) 385, 797, 996
BsmFI GGGAC 1 cut(s) 867
BsnI GGCC 5 cut(s) 256, 605, 985, 994, 1233
BsoBI CYCGRG 1 cut(s) 1234
Bsp1286I GDGCHC 2 cut(s) 395, 1009
Bsp143I GATC 3 cut(s) 547, 551, 745
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 3 cut(s) 609, 943, 1049
BspANI GGCC 5 cut(s) 256, 605, 985, 994, 1233
BspCNI CTCAG 3 cut(s) 212, 383, 645
BspDI ATCGAT 1 cut(s) 550
BspFNI CGCG 1 cut(s) 1051
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 6, 367, 394
BsrI ACTGG 2 cut(s) 623, 791
BssECI CCNNGG 6 cut(s) 357, 752, 798, 956, 995, 1192
BssMI GATC 3 cut(s) 547, 551, 745
BssT1I CCWWGG 4 cut(s) 357, 752, 956, 995
Bst2UI CCWGG 3 cut(s) 991, 1131, 1193
Bst4CI ACNGT 4 cut(s) 146, 416, 436, 558
BstC8I GCNNGC 4 cut(s) 23, 945, 1041, 1231
BstDEI CTNAG 5 cut(s) 199, 370, 653, 962, 1139
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 4 cut(s) 341, 401, 502, 986
BstFNI CGCG 1 cut(s) 1051
BstHHI GCGC 1 cut(s) 1053
BstKTI GATC 3 cut(s) 550, 554, 748
BstMBI GATC 3 cut(s) 547, 551, 745
BstMWI GCNNNNNNNGC 3 cut(s) 22, 991, 1040
BstNI CCWGG 3 cut(s) 991, 1131, 1193
BstNSI RCATGY 2 cut(s) 723, 1043
BstSCI CCNGG 4 cut(s) 797, 989, 1129, 1191
BstSLI GKGCMC 1 cut(s) 1009
BstUI CGCG 1 cut(s) 1051
BstV1I GCAGC 2 cut(s) 641, 1177
BstV2I GAAGAC 2 cut(s) 120, 534
BstXI CCANNNNNNTGG 1 cut(s) 60
Bsu15I ATCGAT 1 cut(s) 550
BsuRI GGCC 5 cut(s) 256, 605, 985, 994, 1233
BsuTUI ATCGAT 1 cut(s) 550
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 4 cut(s) 341, 401, 502, 986
BtsIMutI CAGTG 3 cut(s) 295, 421, 432
Cac8I GCNNGC 4 cut(s) 23, 945, 1041, 1231
CaiI CAGNNNCTG 2 cut(s) 206, 562
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 1053
Cfr13I GGNCC 4 cut(s) 5, 254, 983, 1232
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 2 cut(s) 324, 445
CviAII CATG 9 cut(s) 18, 104, 358, 385, 487, 720, 818, 827, 1040
CviQI GTAC 2 cut(s) 324, 445
DdeI CTNAG 5 cut(s) 199, 370, 653, 962, 1139
DpnI GATC 3 cut(s) 549, 553, 747
DpnII GATC 3 cut(s) 547, 551, 745
DraI TTTAAA 1 cut(s) 1243
EaeI YGGCCR 1 cut(s) 603
Eco130I CCWWGG 4 cut(s) 357, 752, 956, 995
Eco147I AGGCCT 1 cut(s) 994
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 5
Eco57I CTGAAG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 1234
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 989, 1129, 1191
EcoT14I CCWWGG 4 cut(s) 357, 752, 956, 995
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 4 cut(s) 357, 752, 956, 995
FaeI CATG 9 cut(s) 21, 107, 361, 388, 490, 723, 821, 830, 1043
FaqI GGGAC 1 cut(s) 867
FatI CATG 9 cut(s) 17, 103, 357, 384, 486, 719, 817, 826, 1039
FauI CCCGC 2 cut(s) 936, 1056
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 2 cut(s) 681, 1188
Fnu4HI GCNGC 2 cut(s) 630, 1166
FokI GGATG 4 cut(s) 328, 388, 489, 973
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 630, 1166
GlaI GCGC 1 cut(s) 1052
GluI GCNGC 2 cut(s) 630, 1166
GsaI CCCAGC 3 cut(s) 166, 219, 566
GsuI CTGGAG 2 cut(s) 255, 633
HaeIII GGCC 5 cut(s) 256, 605, 985, 994, 1233
HapII CCGG 1 cut(s) 798
HhaI GCGC 1 cut(s) 1053
Hin1II CATG 9 cut(s) 21, 107, 361, 388, 490, 723, 821, 830, 1043
Hin6I GCGC 1 cut(s) 1051
HinP1I GCGC 1 cut(s) 1051
HincII GTYRAC 2 cut(s) 682, 1189
HindII GTYRAC 2 cut(s) 682, 1189
HinfI GANTC 5 cut(s) 422, 539, 580, 655, 1078
HpaII CCGG 1 cut(s) 798
HphI GGTGA 2 cut(s) 36, 182
Hpy166II GTNNAC 3 cut(s) 682, 1007, 1189
Hpy188I TCNGA 9 cut(s) 208, 241, 373, 412, 427, 547, 654, 702, 768
Hpy188III TCNNGA 4 cut(s) 57, 104, 571, 936
Hpy8I GTNNAC 3 cut(s) 682, 1007, 1189
Hpy99I CGWCG 1 cut(s) 768
HpyAV CCTTC 1 cut(s) 1022
HpyCH4III ACNGT 4 cut(s) 146, 416, 436, 558
HpyCH4V TGCA 8 cut(s) 311, 470, 521, 643, 830, 1007, 1039, 1165
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 991, 1040
HpyF3I CTNAG 5 cut(s) 199, 370, 653, 962, 1139
Hsp92II CATG 9 cut(s) 21, 107, 361, 388, 490, 723, 821, 830, 1043
HspAI GCGC 1 cut(s) 1051
Kzo9I GATC 3 cut(s) 547, 551, 745
LmnI GCTCC 4 cut(s) 398, 841, 1048, 1196
Lsp1109I GCAGC 2 cut(s) 641, 1177
MaeIII GTNAC 1 cut(s) 793
MalI GATC 3 cut(s) 549, 553, 747
MboI GATC 3 cut(s) 547, 551, 745
MboII GAAGA 5 cut(s) 95, 120, 444, 534, 917
MhlI GDGCHC 2 cut(s) 395, 1009
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 4 cut(s) 86, 969, 1111, 1146
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 4 cut(s) 431, 548, 664, 1087
MmeI TCCRAC 3 cut(s) 100, 746, 1183
Mox20I TGGCCA 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 731
MscI TGGCCA 1 cut(s) 605
MseI TTAA 3 cut(s) 227, 1071, 1242
MslI CAYNNNNRTG 3 cut(s) 58, 465, 1173
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 1 cut(s) 798
MspR9I CCNGG 4 cut(s) 799, 991, 1131, 1193
MvaI CCWGG 3 cut(s) 991, 1131, 1193
MvnI CGCG 1 cut(s) 1051
MwoI GCNNNNNNNGC 3 cut(s) 22, 991, 1040
NciI CCSGG 1 cut(s) 799
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 466
NdeII GATC 3 cut(s) 547, 551, 745
NlaIII CATG 9 cut(s) 21, 107, 361, 388, 490, 723, 821, 830, 1043
NlaIV GGNNCC 3 cut(s) 6, 367, 394
NspI RCATGY 2 cut(s) 723, 1043
PaeI GCATGC 1 cut(s) 1043
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 994
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 1 cut(s) 580
PflMI CCANNNNNTGG 2 cut(s) 385, 996
PkrI GCNGC 2 cut(s) 631, 1167
PleI GAGTC 4 cut(s) 430, 547, 663, 1086
PpsI GAGTC 4 cut(s) 430, 547, 663, 1086
Psp6I CCWGG 3 cut(s) 989, 1129, 1191
PspFI CCCAGC 3 cut(s) 162, 215, 562
PspGI CCWGG 3 cut(s) 989, 1129, 1191
PspN4I GGNNCC 3 cut(s) 6, 367, 394
PspPI GGNCC 4 cut(s) 5, 254, 983, 1232
PstNI CAGNNNCTG 2 cut(s) 206, 562
RsaI GTAC 2 cut(s) 325, 446
RsaNI GTAC 2 cut(s) 324, 445
RseI CAYNNNNRTG 3 cut(s) 58, 465, 1173
SalI GTCGAC 2 cut(s) 680, 1187
SaqAI TTAA 3 cut(s) 227, 1071, 1242
SatI GCNGC 2 cut(s) 630, 1166
Sau3AI GATC 3 cut(s) 547, 551, 745
Sau96I GGNCC 4 cut(s) 5, 254, 983, 1232
SchI GAGTC 4 cut(s) 431, 548, 664, 1087
ScrFI CCNGG 4 cut(s) 799, 991, 1131, 1193
SduI GDGCHC 2 cut(s) 395, 1009
SfiI GGCCNNNNNGGCC 1 cut(s) 991
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 3 cut(s) 58, 465, 1173
SmlI CTYRAG 2 cut(s) 249, 934
SmoI CTYRAG 2 cut(s) 249, 934
SphI GCATGC 1 cut(s) 1043
Sse9I AATT 4 cut(s) 86, 969, 1111, 1146
SseBI AGGCCT 1 cut(s) 994
SsiI CCGC 3 cut(s) 609, 943, 1049
StuI AGGCCT 1 cut(s) 994
StyD4I CCNGG 4 cut(s) 797, 989, 1129, 1191
StyI CCWWGG 4 cut(s) 357, 752, 956, 995
TaaI ACNGT 4 cut(s) 146, 416, 436, 558
TaqI TCGA 4 cut(s) 550, 681, 748, 1188
TasI AATT 4 cut(s) 86, 969, 1111, 1146
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 3 cut(s) 227, 1071, 1242
Tru9I TTAA 3 cut(s) 227, 1071, 1242
TscAI CASTG 3 cut(s) 295, 421, 439
TseI GCWGC 2 cut(s) 629, 1165
TspDTI ATGAA 6 cut(s) 92, 126, 225, 318, 475, 491
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 3 cut(s) 295, 421, 439
Van91I CCANNNNNTGG 2 cut(s) 385, 996
VneI GTGCAC 1 cut(s) 1005
VpaK11BI GGWCC 1 cut(s) 5
XceI RCATGY 2 cut(s) 723, 1043
XcmI CCANNNNNNNNNTGG 2 cut(s) 993, 1199
XmiI GTMKAC 2 cut(s) 681, 1188
XmnI GAANNNNTTC 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.