MD13G1071900.v1.1

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009601
Physical Location & Seq
Reverse (-)
5052533 .. 5054353
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1071900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGACAGCCTTAAAGCAGAGAAAATTGAGAGCCAAAACCTACCCTCACAGGAACCAACCAAAGGTGGAGGCACCACATTTTTCAGAACATGTTTTAATGGTCTCAACACGTTATCAGGAGTTGGGATTCTATCGATTCCATTTGCGCTTTCTGAAGGAGGGTGGCTGAGCTTAATCCTTCTTTTCCAGTTGGCACTTCTTTGTTGGTATACTGGGTTGCTTCTGCAACGTTGCATGGACGGGAATCCGGGGATCAAGAGTTATCCCGACATTGGAGGAGCTGCTTATGGACAGAAAGGAAGACTTGCCATCTCCATTTTTATGTACCTTGAGCTGTATTTGGTAGCTGTTGAGTTTCTAATATTAGAAGGTGACAACTTAAACCAGTTGTTCCCAAGCATGGGTTTTAACATGGCAGGCCTCAAAGTCGGAGGCAAACAATGCTTTGTTTTGCTCACCGCCCTTGTAATTCTGCCAACGACATGGCTGAAGAGTCTGGGATTGTTGGCGTATGTTTCCGCCGGAGGAGTTTTCGCCTCTCTTTTTTTGGTTGCCTGCGTATTTTGGGTTGGTGCGGTTGATGGTGTAGGGTTTCATGAAGGAGATGTTCTTCTGAATTTCAGAGGATTGCCTACTGCTGTGAGCTTGTATCTGTTCTGCTATTGTGGCCATGCAGTTTTTCCCACATTGTGCAATTCCATGAAGGACAGAAGCAAATTCTCCAAGGTATTACTTATCTGCTTCGTTGCAAGCACCATCACCTACGGATCAATGGCGGTTTTAGGTTACTTGATGTTCGGACAAAACTTGAAGTCTCAAGTCACATTAAATCTTCCAGTACGAAAAATTTGTTCAAGGATTGCAATTTACATTACTATAATTAATCCCCTCACAAAGTATGCAATTATAATCACTCCGATTGCTGCTGCTATTGAGGATACACGCCCTTTTCGCAACAGTCGCACAATTAGCATCTTCGTGCGAACCTTAATTGTAATTAGCACCGTAATTGTGGCATTAGCCATTCCATTTTTCAGCTACTTAATGGCTTTAGTCGGCGCATCTCTGAGCGTGACGGTCTCAGTCTTGCTGCCGTGCTTGTTCTACCTCAAGATTAACGTTGCTGCTCAGAGATTCGGGTTCGAGTTCGTGGTAATTGTTGCGATTATGGTAATAGGGTCTTTTGTTGGTATAGTGGGTACGTACACTTCTGTGAAACAAATTGTAAGACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

413

Amino Acids

44.8

Weight (kDa)

9.13

Isoelectric Point (pI)

25.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 24 - 402 1.1e-63 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 209
AciI CCGC 4 cut(s) 459, 519, 575, 776
AclI AACGTT 2 cut(s) 229, 1119
AclWI GGATC 2 cut(s) 260, 775
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 3 cut(s) 616, 716, 846
AcuI CTGAAG 2 cut(s) 174, 509
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 4 cut(s) 326, 840, 1201, 1205
AfiI CCNNNNNNNGG 4 cut(s) 62, 272, 400, 401
AflIII ACRYGT 2 cut(s) 89, 108
AgsI TTSAA 2 cut(s) 811, 855
AleI CACNNNNGTG 1 cut(s) 1211
AluBI AGCT 6 cut(s) 171, 281, 334, 347, 645, 1038
AluI AGCT 6 cut(s) 171, 281, 334, 347, 645, 1038
Alw26I GTCTC 3 cut(s) 107, 819, 1084
AlwI GGATC 2 cut(s) 260, 775
AoxI GGCC 2 cut(s) 418, 667
ApeKI GCWGC 5 cut(s) 281, 923, 926, 1090, 1124
ApoI RAATTY 3 cut(s) 616, 716, 846
AseI ATTAAT 1 cut(s) 882
AspLEI GCGC 2 cut(s) 148, 1061
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 3 cut(s) 383, 448, 751
BalI TGGCCA 1 cut(s) 669
BanI GGYRCC 1 cut(s) 71
BarI GAAGNNNNNNTAC 2 cut(s) 1192, 1224
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 5 cut(s) 268, 910, 913, 1077, 1111
BccI CCATC 3 cut(s) 317, 575, 764
BceAI ACGGC 1 cut(s) 1078
BciVI GTATCC 1 cut(s) 931
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 3 cut(s) 107, 819, 1084
BfuI GTATCC 1 cut(s) 931
BisI GCNGC 5 cut(s) 282, 924, 927, 1091, 1125
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 5 cut(s) 283, 925, 928, 1092, 1126
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 54, 73
BmrFI CCNGG 1 cut(s) 249
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 981, 1070
BmuI ACTGGG 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 167
BpuEI CTTGAG 3 cut(s) 350, 801, 1094
BpuMI CCSGG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 1203
BsaI GGTCTC 2 cut(s) 107, 1084
BsaJI CCNNGG 2 cut(s) 248, 723
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 272, 400, 401
Bse1I ACTGG 4 cut(s) 187, 217, 385, 836
BseCI ATCGAT 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 248, 723
BseLI CCNNNNNNNGG 4 cut(s) 62, 272, 400, 401
BseMII CTCAG 4 cut(s) 158, 1058, 1095, 1142
BseNI ACTGG 4 cut(s) 187, 217, 385, 836
BseRI GAGGAG 2 cut(s) 291, 540
BseXI GCAGC 5 cut(s) 268, 910, 913, 1077, 1111
BshFI GGCC 2 cut(s) 420, 669
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 134
BsiSI CCGG 2 cut(s) 248, 522
BslI CCNNNNNNNGG 4 cut(s) 62, 272, 400, 401
BsmAI GTCTC 3 cut(s) 107, 819, 1084
BsnI GGCC 2 cut(s) 420, 669
Bso31I GGTCTC 2 cut(s) 107, 1084
Bsp143I GATC 2 cut(s) 252, 767
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 4 cut(s) 459, 519, 575, 776
BspANI GGCC 2 cut(s) 420, 669
BspCNI CTCAG 4 cut(s) 159, 1059, 1094, 1141
BspDI ATCGAT 1 cut(s) 134
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 54, 73
BspPI GGATC 2 cut(s) 260, 775
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 2 cut(s) 107, 1084
BsrI ACTGG 4 cut(s) 187, 217, 385, 836
BssECI CCNNGG 2 cut(s) 248, 723
BssMI GATC 2 cut(s) 252, 767
BssNAI GTATAC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 723
Bst1107I GTATAC 1 cut(s) 210
Bst4CI ACNGT 3 cut(s) 959, 1006, 1078
Bst6I CTCTTC 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 1203
BstC8I GCNNGC 3 cut(s) 418, 556, 751
BstDEI CTNAG 4 cut(s) 167, 1067, 1081, 1128
BstHHI GCGC 2 cut(s) 148, 1061
BstKTI GATC 2 cut(s) 255, 770
BstMAI GTCTC 3 cut(s) 107, 819, 1084
BstMBI GATC 2 cut(s) 252, 767
BstMWI GCNNNNNNNGC 5 cut(s) 441, 666, 951, 960, 969
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 247
BstSNI TACGTA 1 cut(s) 1203
BstV1I GCAGC 5 cut(s) 268, 910, 913, 1077, 1111
BstV2I GAAGAC 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 483
BstZ17I GTATAC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 134
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 2 cut(s) 420, 669
BsuTUI ATCGAT 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 418, 556, 751
CciI TCATGA 1 cut(s) 595
CfoI GCGC 2 cut(s) 148, 1061
ClaI ATCGAT 1 cut(s) 134
Csp6I GTAC 4 cut(s) 325, 839, 1200, 1204
CviAII CATG 8 cut(s) 90, 235, 400, 412, 483, 596, 671, 700
CviQI GTAC 4 cut(s) 325, 839, 1200, 1204
DdeI CTNAG 4 cut(s) 167, 1067, 1081, 1128
DpnI GATC 2 cut(s) 254, 769
DpnII GATC 2 cut(s) 252, 767
DraIII CACNNNGTG 1 cut(s) 690
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
EciI GGCGGA 1 cut(s) 508
Eco105I TACGTA 1 cut(s) 1203
Eco130I CCWWGG 1 cut(s) 723
Eco147I AGGCCT 1 cut(s) 420
Eco31I GGTCTC 2 cut(s) 107, 1084
Eco57I CTGAAG 2 cut(s) 174, 509
EcoT14I CCWWGG 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 8 cut(s) 89, 234, 399, 411, 482, 595, 670, 699
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 5 cut(s) 282, 924, 927, 1091, 1125
Fsp4HI GCNGC 5 cut(s) 282, 924, 927, 1091, 1125
GlaI GCGC 2 cut(s) 147, 1060
GluI GCNGC 5 cut(s) 282, 924, 927, 1091, 1125
HaeIII GGCC 2 cut(s) 420, 669
HapII CCGG 2 cut(s) 248, 522
HhaI GCGC 2 cut(s) 148, 1061
Hin1II CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
Hin6I GCGC 2 cut(s) 146, 1059
HinP1I GCGC 2 cut(s) 146, 1059
HinfI GANTC 5 cut(s) 127, 136, 244, 493, 1134
HpaII CCGG 2 cut(s) 248, 522
HphI GGTGA 3 cut(s) 383, 448, 751
Hpy166II GTNNAC 2 cut(s) 210, 1206
Hpy188III TCNNGA 5 cut(s) 117, 256, 266, 596, 1111
Hpy8I GTNNAC 2 cut(s) 210, 1206
HpyAV CCTTC 5 cut(s) 149, 188, 362, 593, 697
HpyCH4III ACNGT 3 cut(s) 959, 1006, 1078
HpyCH4IV ACGT 4 cut(s) 110, 229, 1119, 1202
HpyCH4V TGCA 7 cut(s) 226, 234, 674, 693, 749, 863, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 441, 666, 951, 960, 969
HpyF3I CTNAG 4 cut(s) 167, 1067, 1081, 1128
HpySE526I ACGT 4 cut(s) 110, 229, 1119, 1202
Hsp92II CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
HspAI GCGC 2 cut(s) 146, 1059
Kzo9I GATC 2 cut(s) 252, 767
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 5 cut(s) 268, 910, 913, 1077, 1111
LweI GCATC 2 cut(s) 981, 1070
MaeII ACGT 4 cut(s) 110, 229, 1119, 1202
MaeIII GTNAC 4 cut(s) 371, 785, 820, 1072
MalI GATC 2 cut(s) 254, 769
MboI GATC 2 cut(s) 252, 767
MboII GAAGA 5 cut(s) 312, 502, 602, 824, 967
MlsI TGGCCA 1 cut(s) 669
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 409
Mox20I TGGCCA 1 cut(s) 669
MscI TGGCCA 1 cut(s) 669
MslI CAYNNNNRTG 3 cut(s) 320, 977, 1211
Msp20I TGGCCA 1 cut(s) 669
MspI CCGG 2 cut(s) 248, 522
MspR9I CCNGG 1 cut(s) 249
MwoI GCNNNNNNNGC 5 cut(s) 441, 666, 951, 960, 969
NciI CCSGG 1 cut(s) 249
NdeII GATC 2 cut(s) 252, 767
NlaIII CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
NlaIV GGNNCC 2 cut(s) 54, 73
NmuCI GTSAC 3 cut(s) 371, 820, 1072
NspI RCATGY 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 595
PceI AGGCCT 1 cut(s) 420
PciI ACATGT 1 cut(s) 89
PfeI GAWTC 4 cut(s) 127, 136, 244, 1134
PkrI GCNGC 5 cut(s) 283, 925, 928, 1092, 1126
PleI GAGTC 1 cut(s) 501
PpsI GAGTC 1 cut(s) 501
Ppu21I YACGTR 1 cut(s) 1203
PscI ACATGT 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 882
PsiI TTATAA 1 cut(s) 908
Psp1406I AACGTT 2 cut(s) 229, 1119
PspN4I GGNNCC 2 cut(s) 54, 73
RsaI GTAC 4 cut(s) 326, 840, 1201, 1205
RsaNI GTAC 4 cut(s) 325, 839, 1200, 1204
RseI CAYNNNNRTG 3 cut(s) 320, 977, 1211
SatI GCNGC 5 cut(s) 282, 924, 927, 1091, 1125
Sau3AI GATC 2 cut(s) 252, 767
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 2 cut(s) 981, 1070
SmiMI CAYNNNNRTG 3 cut(s) 320, 977, 1211
SmlI CTYRAG 3 cut(s) 329, 816, 1109
SmoI CTYRAG 3 cut(s) 329, 816, 1109
SnaBI TACGTA 1 cut(s) 1203
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 4 cut(s) 459, 519, 575, 776
SspI AATATT 1 cut(s) 363
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 3 cut(s) 959, 1006, 1078
TaiI ACGT 4 cut(s) 113, 232, 1122, 1205
TaqI TCGA 2 cut(s) 134, 1143
TfiI GAWTC 4 cut(s) 127, 136, 244, 1134
TseFI GTSAC 3 cut(s) 371, 820, 1072
TseI GCWGC 5 cut(s) 281, 923, 926, 1090, 1124
Tsp45I GTSAC 3 cut(s) 371, 820, 1072
TspDTI ATGAA 3 cut(s) 584, 612, 716
TspGWI ACGGA 1 cut(s) 780
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 3 cut(s) 616, 716, 846
XceI RCATGY 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.