Rw4G031830

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009601
Physical Location & Seq
Reverse (-)
59001461 .. 59002888
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031830.1

Sequence Viewer

Length: 1239 bp
ATGGAGTTGGACTCCCTCAAAGTAGAGAAGATAGAGAGCCAAATTCAACCACTTGAACCAAACAAAGGCACCACCTTTTTCAGAACCTGTTTCAATGGACTCAACACACTGTCAGGTGTGGGAATACTATCAGTTCCATTTGCTCTATCTCAGGGAGGGTGGTTGAGCTTAGTGCTTCTCTTCTCGGTAGCAATCCTATGTTGGTATACAGGGCTGCTTCTACAGCGATGTATGGACTCAAATTCGCACATAAAAACTTATCCCGACATTGGTCAAGCTGCTTTTGGACGCAAAGGAAGAGTGGCCATATCCATCTTTATGTATCTTGAGCTGTATTTAGTTGCAGTTGAATTTCTAATACTAGAAGGTGATAACTTAAACCAGTTGTTTCCAAACGTGAGTTTTAACATGGGAGGCCACACAATTGGAGGTAAGCAATGCTTTGTTTTAGCCACTGCCCTTGTAATTCTGCCGACTACATGGTTGAAGAGCTTGGGATTGCTGGCATATATTTCAGCTGGAGGGGTTTTGGCTTCGATTATTTTGGTTGCTTGTGTTTTTTGGGTTGGTGCTGTTGATAGTGTGGGATTTCATGAAAGAGGTGATCTCCTGAATTGGAAAGGATTGCCTACTGCTATAAGCTTGTATTTGTTCTGTTACTGTGGTCATGCAGTTTTTCCCACATTATGCAATTCCATGAAAGATAGAAGCCAATTCCCCAAGGTATTGCTTGTTTGCTTCATAACAAGCACCATCACCTATGGATCCATGGCCGTTTTGGGTTACTTGATGTTTGGAGAATATTTGAAGTCCCAAGTGACATTAAATCTTCCTGTACAAAAAATTAGTGCTAAAGTAGCAATTTTCATCACAATAGTTAACCCCTTGACTAAGTATGCAATTATAATCACTCCAATTGCTACTGCTATAGAGGACTCACGCTATTTTTGCAACAGTCATGCTATTATCAGCATCATGGTGAGGACGGGGATTGTGATCAGCACCGTATGTGTGGCATTGATGGTTCCATTTTTCGGTTATGTCATGGCATTCGTCGGTGCTTTTCTGAGTGTAACTGTCTCAATATTGTTGCCGTGCCTGTTCTACCTGAAGATTAACAATGTTGCTCTCAGGTTTGGGTTCGAGTTGATGACAATTGTTGGGATTATGGTGATGGGATCTTTTGTTGGCGTAGTAGGCACTTACACTTCTCTGAAAGAAATTGTAAACCATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

412

Amino Acids

45.07

Weight (kDa)

8.53

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 23 - 402 9.5e-67 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 206
AclWI GGATC 3 cut(s) 759, 772, 1186
AcoI YGGCCR 2 cut(s) 303, 771
AcsI RAATTY 3 cut(s) 42, 241, 350
AcuI CTGAAG 1 cut(s) 1130
AfaI GTAC 1 cut(s) 837
AfiI CCNNNNNNNGG 3 cut(s) 65, 269, 1034
AgsI TTSAA 6 cut(s) 47, 56, 94, 350, 487, 808
AluBI AGCT 6 cut(s) 168, 278, 331, 492, 518, 642
AluI AGCT 6 cut(s) 168, 278, 331, 492, 518, 642
Alw26I GTCTC 1 cut(s) 1084
AlwI GGATC 3 cut(s) 759, 772, 1186
AlwNI CAGNNNCTG 1 cut(s) 87
AoxI GGCC 3 cut(s) 303, 415, 771
ApeKI GCWGC 2 cut(s) 214, 278
ApoI RAATTY 3 cut(s) 42, 241, 350
AsuHPI GGTGA 5 cut(s) 380, 614, 748, 991, 1183
BalI TGGCCA 1 cut(s) 305
BamHI GGATCC 1 cut(s) 764
BanI GGYRCC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 201, 265
BccI CCATC 4 cut(s) 320, 761, 1015, 1168
BceAI ACGGC 2 cut(s) 758, 1078
BclI TGATCA 1 cut(s) 996
BcoDI GTCTC 1 cut(s) 1084
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 2 cut(s) 221, 927
BisI GCNGC 2 cut(s) 215, 279
BlsI GCNGC 2 cut(s) 216, 280
BmiI GGNNCC 3 cut(s) 70, 766, 1026
BmsI GCATC 1 cut(s) 981
BoxI GACNNNNGTC 1 cut(s) 270
BplI GAGNNNNNCTC 3 cut(s) 28, 591, 623
BpmI CTGGAG 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 995
BsaJI CCNNGG 2 cut(s) 720, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 269, 1034
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 443
Bse8I GATNNNNATC 1 cut(s) 995
BseDI CCNNGG 2 cut(s) 720, 768
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 3 cut(s) 65, 269, 1034
BseMI GCAATG 1 cut(s) 443
BseMII CTCAG 3 cut(s) 164, 1058, 1144
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 2 cut(s) 201, 265
BshFI GGCC 3 cut(s) 305, 417, 773
BshNI GGYRCC 1 cut(s) 68
BslFI GGGAC 1 cut(s) 796
BslI CCNNNNNNNGG 3 cut(s) 65, 269, 1034
BsmAI GTCTC 1 cut(s) 1084
BsmFI GGGAC 1 cut(s) 796
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 3 cut(s) 305, 417, 773
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 4 cut(s) 604, 764, 996, 1178
Bsp19I CCATGG 1 cut(s) 768
BspANI GGCC 3 cut(s) 305, 417, 773
BspCNI CTCAG 3 cut(s) 163, 1059, 1143
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 3 cut(s) 70, 766, 1026
BspPI GGATC 3 cut(s) 759, 772, 1186
BspQI GCTCTTC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 443
BsrGI TGTACA 1 cut(s) 835
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 720, 768
BssMI GATC 4 cut(s) 604, 764, 996, 1178
BssNAI GTATAC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 720, 768
Bst1107I GTATAC 1 cut(s) 207
Bst4CI ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
Bst6I CTCTTC 3 cut(s) 185, 292, 482
BstAUI TGTACA 1 cut(s) 835
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 5 cut(s) 150, 169, 891, 1067, 1130
BstDSI CCRYGG 1 cut(s) 768
BstKTI GATC 4 cut(s) 607, 767, 999, 1181
BstMAI GTCTC 1 cut(s) 1084
BstMBI GATC 4 cut(s) 604, 764, 996, 1178
BstMWI GCNNNNNNNGC 4 cut(s) 223, 857, 948, 1197
BstPAI GACNNNNGTC 1 cut(s) 270
BstSFI CTRYAG 2 cut(s) 221, 927
BstV1I GCAGC 2 cut(s) 201, 265
BstX2I RGATCY 2 cut(s) 764, 1178
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 2 cut(s) 764, 1178
BstZ17I GTATAC 1 cut(s) 207
BsuRI GGCC 3 cut(s) 305, 417, 773
BtgI CCRYGG 1 cut(s) 768
BtgZI GCGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 107, 453
Cac8I GCNNGC 1 cut(s) 504
CaiI CAGNNNCTG 1 cut(s) 87
CciI TCATGA 1 cut(s) 592
CseI GACGC 1 cut(s) 297
Csp6I GTAC 1 cut(s) 836
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 9 cut(s) 409, 480, 593, 668, 697, 769, 959, 976, 1045
CviQI GTAC 1 cut(s) 836
DdeI CTNAG 5 cut(s) 150, 169, 891, 1067, 1130
DpnI GATC 4 cut(s) 606, 766, 998, 1180
DpnII GATC 4 cut(s) 604, 764, 996, 1178
EaeI YGGCCR 2 cut(s) 303, 771
Eam1104I CTCTTC 3 cut(s) 185, 292, 482
EarI CTCTTC 3 cut(s) 185, 292, 482
Eco130I CCWWGG 2 cut(s) 720, 768
Eco57I CTGAAG 1 cut(s) 1130
EcoT14I CCWWGG 2 cut(s) 720, 768
ErhI CCWWGG 2 cut(s) 720, 768
FaeI CATG 9 cut(s) 412, 483, 596, 671, 700, 772, 962, 979, 1048
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 796
FatI CATG 9 cut(s) 408, 479, 592, 667, 696, 768, 958, 975, 1044
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 215, 279
Fsp4HI GCNGC 2 cut(s) 215, 279
FspBI CTAG 1 cut(s) 362
GluI GCNGC 2 cut(s) 215, 279
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 3 cut(s) 305, 417, 773
HgaI GACGC 1 cut(s) 297
Hin1II CATG 9 cut(s) 412, 483, 596, 671, 700, 772, 962, 979, 1048
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 4 cut(s) 11, 99, 236, 935
HpaI GTTAAC 1 cut(s) 880
HphI GGTGA 5 cut(s) 380, 614, 748, 991, 1183
Hpy166II GTNNAC 3 cut(s) 207, 880, 1228
Hpy188I TCNGA 3 cut(s) 83, 1068, 1215
Hpy188III TCNNGA 4 cut(s) 263, 326, 593, 610
Hpy8I GTNNAC 3 cut(s) 207, 880, 1228
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 5 cut(s) 344, 671, 690, 899, 951
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 857, 948, 1197
HpyF3I CTNAG 5 cut(s) 150, 169, 891, 1067, 1130
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 9 cut(s) 412, 483, 596, 671, 700, 772, 962, 979, 1048
Ksp22I TGATCA 1 cut(s) 996
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 4 cut(s) 604, 764, 996, 1178
LguI GCTCTTC 1 cut(s) 482
Lsp1109I GCAGC 2 cut(s) 201, 265
LweI GCATC 1 cut(s) 981
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 656, 782, 817, 1072
MalI GATC 4 cut(s) 606, 766, 998, 1180
MboI GATC 4 cut(s) 604, 764, 996, 1178
MboII GAAGA 6 cut(s) 40, 172, 309, 499, 821, 1123
MfeI CAATTG 3 cut(s) 423, 915, 1155
MflI RGATCY 2 cut(s) 764, 1178
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 4 cut(s) 5, 93, 230, 929
MnlI CCTC 8 cut(s) 26, 149, 407, 422, 515, 593, 925, 975
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 5 cut(s) 377, 405, 824, 879, 1116
MslI CAYNNNNRTG 2 cut(s) 317, 977
Msp20I TGGCCA 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 518
MunI CAATTG 3 cut(s) 423, 915, 1155
Mva1269I GAATGC 1 cut(s) 1049
MwoI GCNNNNNNNGC 4 cut(s) 223, 857, 948, 1197
NcoI CCATGG 1 cut(s) 768
NdeII GATC 4 cut(s) 604, 764, 996, 1178
NlaIII CATG 9 cut(s) 412, 483, 596, 671, 700, 772, 962, 979, 1048
NlaIV GGNNCC 3 cut(s) 70, 766, 1026
NmuCI GTSAC 1 cut(s) 817
PagI TCATGA 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 482
PctI GAATGC 1 cut(s) 1049
PkrI GCNGC 2 cut(s) 216, 280
PleI GAGTC 4 cut(s) 5, 93, 230, 929
PpsI GAGTC 4 cut(s) 5, 93, 230, 929
PshAI GACNNNNGTC 1 cut(s) 270
PsiI TTATAA 1 cut(s) 905
PspN4I GGNNCC 3 cut(s) 70, 766, 1026
PstNI CAGNNNCTG 1 cut(s) 87
PsuI RGATCY 2 cut(s) 764, 1178
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 837
RsaNI GTAC 1 cut(s) 836
RseI CAYNNNNRTG 2 cut(s) 317, 977
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 5 cut(s) 377, 405, 824, 879, 1116
SatI GCNGC 2 cut(s) 215, 279
Sau3AI GATC 4 cut(s) 604, 764, 996, 1178
SchI GAGTC 4 cut(s) 5, 93, 230, 929
SfaNI GCATC 1 cut(s) 981
SfcI CTRYAG 2 cut(s) 221, 927
SmiMI CAYNNNNRTG 2 cut(s) 317, 977
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SspI AATATT 2 cut(s) 803, 1086
SspMI CTAG 1 cut(s) 362
StyI CCWWGG 2 cut(s) 720, 768
TaaI ACNGT 5 cut(s) 111, 662, 956, 1006, 1078
TaiI ACGT 1 cut(s) 399
TaqI TCGA 2 cut(s) 536, 1143
TatI WGTACW 1 cut(s) 835
Tru1I TTAA 5 cut(s) 377, 405, 824, 879, 1116
Tru9I TTAA 5 cut(s) 377, 405, 824, 879, 1116
TscAI CASTG 2 cut(s) 114, 460
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 2 cut(s) 214, 278
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 5 cut(s) 581, 609, 713, 730, 856
TspRI CASTG 2 cut(s) 114, 460
XapI RAATTY 3 cut(s) 42, 241, 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.