Rorug04G0321500

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009601
Physical Location & Seq
Reverse (-)
49599737 .. 49601126
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0321500.1

Sequence Viewer

Length: 381 bp
ATGGATCCGACCCTGCAAAGGTTTGAGTTGAATCTCGATTCTTCTCAAGTTGTCTTCAGTCCTTCAAGCCCACCCCAACTCCCAGTTCTTCAGTTACTTCAAGGTTGCTGCGTACTGAAGAGCTTCACTCCTTCAATCCTTCAAGCCCACCCAGAGTCCCGACCAAGCAACCAGGTGACCACGATCGTCGAGCAAGGAGCAAGGTGCTGCATTCCTTTCTCTTCTTCTCTCAGAGTCTTAGAGATCGAAGGCTCCTTCTCTTCTACTCTTCTTCTTCTTTGGGGCAATCAATTGAATGTAGTGGTCTGGAACATGCTCTCTTGCTTACAATTTATTGTGGGTTTCTCCTTCTCTTTCCTCCAATTCGATGTGAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

126

Amino Acids

13.98

Weight (kDa)

4.79

Isoelectric Point (pI)

68.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcuI CTGAAG 3 cut(s) 40, 74, 137
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 6 cut(s) 31, 66, 101, 135, 143, 295
AjnI CCWGG 1 cut(s) 171
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 1 cut(s) 123
AluI AGCT 1 cut(s) 123
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 108, 207
Asp700I GAANNNNTTC 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 187
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 95, 194
BciT130I CCWGG 1 cut(s) 173
BisI GCNGC 2 cut(s) 109, 208
BlsI GCNGC 2 cut(s) 110, 209
Bme1390I CCNGG 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 6, 253
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 77
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpuEI CTTGAG 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 83
BseXI GCAGC 2 cut(s) 95, 194
Bsh1285I CGRYCG 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 210
Bsp143I GATC 3 cut(s) 4, 183, 243
BspCNI CTCAG 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 6, 253
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 83
BssMI GATC 3 cut(s) 4, 183, 243
Bst2UI CCWGG 1 cut(s) 173
Bst6I CTCTTC 4 cut(s) 113, 226, 265, 273
BstDEI CTNAG 2 cut(s) 230, 238
BstEII GGTNACC 1 cut(s) 175
BstKTI GATC 3 cut(s) 7, 186, 246
BstMBI GATC 3 cut(s) 4, 183, 243
BstMCI CGRYCG 1 cut(s) 186
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 95, 194
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
CsiI ACCWGGT 1 cut(s) 171
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 313
CviJI RGCY 4 cut(s) 69, 123, 146, 252
CviKI_1 RGCY 4 cut(s) 69, 123, 146, 252
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 2 cut(s) 230, 238
DpnI GATC 3 cut(s) 6, 185, 245
DpnII GATC 3 cut(s) 4, 183, 243
Eam1104I CTCTTC 4 cut(s) 113, 226, 265, 273
EarI CTCTTC 4 cut(s) 113, 226, 265, 273
Eco57I CTGAAG 3 cut(s) 40, 74, 137
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 171
FaeI CATG 1 cut(s) 316
FaiI YATR 1 cut(s) 314
FaqI GGGAC 1 cut(s) 142
FatI CATG 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 109, 208
Fsp4HI GCNGC 2 cut(s) 109, 208
GluI GCNGC 2 cut(s) 109, 208
Hin1II CATG 1 cut(s) 316
HinfI GANTC 4 cut(s) 31, 38, 155, 234
HphI GGTGA 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 9, 233
Hpy188III TCNNGA 3 cut(s) 35, 159, 307
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 6 cut(s) 72, 141, 149, 242, 265, 358
HpyCH4V TGCA 2 cut(s) 16, 210
HpyF3I CTNAG 2 cut(s) 230, 238
Hsp92II CATG 1 cut(s) 316
Kzo9I GATC 3 cut(s) 4, 183, 243
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 197, 257
LpnPI CCDG 6 cut(s) 26, 96, 158, 165, 185, 292
Lsp1109I GCAGC 2 cut(s) 95, 194
MabI ACCWGGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 93, 175
MalI GATC 3 cut(s) 6, 185, 245
MboI GATC 3 cut(s) 4, 183, 243
MfeI CAATTG 1 cut(s) 290
MflI RGATCY 1 cut(s) 4
MluCI AATT 3 cut(s) 290, 329, 362
MlyI GAGTC 2 cut(s) 164, 243
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 1 cut(s) 173
NdeII GATC 3 cut(s) 4, 183, 243
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 2 cut(s) 6, 253
NmuCI GTSAC 1 cut(s) 175
NspI RCATGY 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 31, 38
PkrI GCNGC 2 cut(s) 110, 209
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 2 cut(s) 163, 242
PpsI GAGTC 2 cut(s) 163, 242
Psp6I CCWGG 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 175
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 6, 253
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 186
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 113
SatI GCNGC 2 cut(s) 109, 208
Sau3AI GATC 3 cut(s) 4, 183, 243
SchI GAGTC 2 cut(s) 164, 243
ScrFI CCNGG 1 cut(s) 173
SetI ASST 5 cut(s) 23, 106, 125, 177, 206
SexAI ACCWGGT 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
Sse9I AATT 3 cut(s) 290, 329, 362
StyD4I CCNGG 1 cut(s) 171
TaqI TCGA 4 cut(s) 36, 189, 246, 366
TasI AATT 3 cut(s) 290, 329, 362
TfiI GAWTC 2 cut(s) 31, 38
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 108, 207
Tsp45I GTSAC 1 cut(s) 175
XceI RCATGY 1 cut(s) 316
XmnI GAANNNNTTC 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.