Rh4CG399200

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009601
Physical Location & Seq
Reverse (-)
64826415 .. 64828699
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG399200.1

Sequence Viewer

Length: 1167 bp
ATGTCTTTAACTCGCTTAATCCATTACCTTGTTAATTTCTCAGGTGTGGGAATACTATCAATTCCATTTGCTCTATCTCAGGGAGGGTGGTTGAGCTTAGTGCTTCTCTTCTCGATAGCAATCCTATGTTGGTATACAGGGCTGCTTCTACAGCGATGTATGGACTCAAATCCGCACATAAAAACTTATCCCGACATTGGTCAAGCTGCTTTTGGACGCAAAGGAAGAGTGGCCATATCCATCTTTATGTATCTTGAGCTGTATTTAGTTGCAGTTGAATTTCTAATACTAGAAGGTGATAACTTAAACCAGTTGTTTCCAAACGTGAGTTTTAACATGGGAGGCCACACAATTGGAGGTAAGCAATGCTTTGTTTTAGCCACTGCCCTTGTAATACTGCCAACTACATGGTTGAAGAGCTTGGGATTGCTGGCATATATTTCAGCTGGAGGGGTTTTGGCTTCGATTATTTTGGTTGCTTGTGTTTTTTGGGTTGGTGCTATTGATAATGTGGGATTTCATGAAAGAGGTGATCTCCTGAATTGGAAAGGATTGCCTACTGCTATTAGCTTGTATTTATTCTGTTACTGTGGTCATGCAGTTTTTCCCACATTATGCAATTCCATGAAAGATAGAAGCCAATTCCCCAAGGTATTGCTTGTTTGCTTCATAACAAGCACCATCACCTATGGATCCATGGCCGTTTTGGGTTACTTGATGTTTGGAGAATATTTGAAGTCCCAAGTGACATTAAATCTTCCTGTACAAAAAATTAGTGCAAAAGTAGCAATTTTCATCACAATAGTTAACCCCCTGACTAAGTATGCAATTATAATCACTCCAATTGCTACTGCTATAGAGGACTCACGCCATTTTTGCAACAGTCATGCTATTATCAGCATCATGGTGAGGACGGGGATTGTGATCAGCACCGTATGTGTGGCATTGATGGTTCCATTTTTCGGCTATGTCATGGCATTCGTCGGTGCTTTTCTGAGTGTAACTGTCTCAGTATTATTGCCGTGCCTGTTCTACTTGAAGATTAACAATGTTGCTCGTAGGTTTGACTTCGAGTTGATGACAATTGTTGGGATTATGGTGATGGGATCTTTTGTTGGCGTAGTGGGCACTTACACTTCTCTGAAAGAAATTGTAAACCATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

388

Amino Acids

42.57

Weight (kDa)

8.81

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 6 - 378 3.8e-67 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccI GTMKAC 1 cut(s) 134
AciI CCGC 1 cut(s) 173
AclWI GGATC 3 cut(s) 687, 700, 1114
AcoI YGGCCR 2 cut(s) 231, 699
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 197, 962
AgsI TTSAA 4 cut(s) 278, 415, 736, 1039
AluBI AGCT 6 cut(s) 96, 206, 259, 420, 446, 570
AluI AGCT 6 cut(s) 96, 206, 259, 420, 446, 570
Alw26I GTCTC 1 cut(s) 1012
AlwI GGATC 3 cut(s) 687, 700, 1114
AoxI GGCC 3 cut(s) 231, 343, 699
ApeKI GCWGC 2 cut(s) 142, 206
ApoI RAATTY 1 cut(s) 278
AsuHPI GGTGA 5 cut(s) 308, 542, 676, 919, 1111
BaeGI GKGCMC 1 cut(s) 1130
BalI TGGCCA 1 cut(s) 233
BamHI GGATCC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 129, 193
BccI CCATC 4 cut(s) 248, 689, 943, 1096
BceAI ACGGC 2 cut(s) 686, 1006
BclI TGATCA 1 cut(s) 924
BcoDI GTCTC 1 cut(s) 1012
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 2 cut(s) 149, 855
BisI GCNGC 2 cut(s) 143, 207
BlsI GCNGC 2 cut(s) 144, 208
BmiI GGNNCC 2 cut(s) 694, 954
BmsI GCATC 1 cut(s) 909
BoxI GACNNNNGTC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 519, 551
BpmI CTGGAG 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 275
BsaBI GATNNNNATC 2 cut(s) 119, 923
BsaJI CCNNGG 2 cut(s) 648, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 962
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 371
Bse8I GATNNNNATC 2 cut(s) 119, 923
BseDI CCNNGG 2 cut(s) 648, 696
BseJI GATNNNNATC 2 cut(s) 119, 923
BseLI CCNNNNNNNGG 2 cut(s) 197, 962
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 4 cut(s) 54, 92, 986, 1023
BseNI ACTGG 1 cut(s) 310
BseSI GKGCMC 1 cut(s) 1130
BseXI GCAGC 2 cut(s) 129, 193
BshFI GGCC 3 cut(s) 233, 345, 701
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 2 cut(s) 197, 962
BsmAI GTCTC 1 cut(s) 1012
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 3 cut(s) 233, 345, 701
Bsp1286I GDGCHC 1 cut(s) 1130
Bsp1407I TGTACA 1 cut(s) 763
Bsp143I GATC 4 cut(s) 532, 692, 924, 1106
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 1 cut(s) 173
BspANI GGCC 3 cut(s) 233, 345, 701
BspCNI CTCAG 4 cut(s) 53, 91, 987, 1022
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 2 cut(s) 694, 954
BspPI GGATC 3 cut(s) 687, 700, 1114
BspQI GCTCTTC 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 763
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 648, 696
BssMI GATC 4 cut(s) 532, 692, 924, 1106
BssNAI GTATAC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 648, 696
Bst1107I GTATAC 1 cut(s) 135
Bst4CI ACNGT 4 cut(s) 590, 884, 934, 1006
Bst6I CTCTTC 3 cut(s) 113, 220, 410
BstAUI TGTACA 1 cut(s) 763
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 6 cut(s) 40, 78, 97, 819, 995, 1009
BstDSI CCRYGG 1 cut(s) 696
BstKTI GATC 4 cut(s) 535, 695, 927, 1109
BstMAI GTCTC 1 cut(s) 1012
BstMBI GATC 4 cut(s) 532, 692, 924, 1106
BstMWI GCNNNNNNNGC 4 cut(s) 151, 785, 876, 1125
BstPAI GACNNNNGTC 1 cut(s) 198
BstSFI CTRYAG 2 cut(s) 149, 855
BstSLI GKGCMC 1 cut(s) 1130
BstV1I GCAGC 2 cut(s) 129, 193
BstX2I RGATCY 2 cut(s) 692, 1106
BstXI CCANNNNNNTGG 2 cut(s) 353, 408
BstYI RGATCY 2 cut(s) 692, 1106
BstZ17I GTATAC 1 cut(s) 135
BsuRI GGCC 3 cut(s) 233, 345, 701
BtgI CCRYGG 1 cut(s) 696
BtgZI GCGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 1 cut(s) 520
CseI GACGC 1 cut(s) 225
Csp6I GTAC 1 cut(s) 764
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 9 cut(s) 337, 408, 521, 596, 625, 697, 887, 904, 973
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 6 cut(s) 40, 78, 97, 819, 995, 1009
DpnI GATC 4 cut(s) 534, 694, 926, 1108
DpnII GATC 4 cut(s) 532, 692, 924, 1106
EaeI YGGCCR 2 cut(s) 231, 699
Eam1104I CTCTTC 3 cut(s) 113, 220, 410
EarI CTCTTC 3 cut(s) 113, 220, 410
Eco130I CCWWGG 2 cut(s) 648, 696
EcoT14I CCWWGG 2 cut(s) 648, 696
ErhI CCWWGG 2 cut(s) 648, 696
FaeI CATG 9 cut(s) 340, 411, 524, 599, 628, 700, 890, 907, 976
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 1 cut(s) 724
FatI CATG 9 cut(s) 336, 407, 520, 595, 624, 696, 886, 903, 972
FbaI TGATCA 1 cut(s) 924
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 2 cut(s) 143, 207
Fsp4HI GCNGC 2 cut(s) 143, 207
FspBI CTAG 1 cut(s) 290
GluI GCNGC 2 cut(s) 143, 207
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 3 cut(s) 233, 345, 701
HgaI GACGC 1 cut(s) 225
Hin1II CATG 9 cut(s) 340, 411, 524, 599, 628, 700, 890, 907, 976
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HinfI GANTC 2 cut(s) 164, 863
HpaI GTTAAC 1 cut(s) 808
HphI GGTGA 5 cut(s) 308, 542, 676, 919, 1111
Hpy166II GTNNAC 3 cut(s) 135, 808, 1156
Hpy188I TCNGA 2 cut(s) 996, 1143
Hpy188III TCNNGA 5 cut(s) 112, 191, 254, 521, 538
Hpy8I GTNNAC 3 cut(s) 135, 808, 1156
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 4 cut(s) 590, 884, 934, 1006
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 6 cut(s) 272, 599, 618, 779, 827, 879
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 785, 876, 1125
HpyF3I CTNAG 6 cut(s) 40, 78, 97, 819, 995, 1009
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 9 cut(s) 340, 411, 524, 599, 628, 700, 890, 907, 976
Ksp22I TGATCA 1 cut(s) 924
KspAI GTTAAC 1 cut(s) 808
Kzo9I GATC 4 cut(s) 532, 692, 924, 1106
LguI GCTCTTC 1 cut(s) 410
Lsp1109I GCAGC 2 cut(s) 129, 193
LweI GCATC 1 cut(s) 909
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 4 cut(s) 584, 710, 745, 1000
MalI GATC 4 cut(s) 534, 694, 926, 1108
MboI GATC 4 cut(s) 532, 692, 924, 1106
MboII GAAGA 5 cut(s) 100, 237, 427, 749, 1051
MfeI CAATTG 3 cut(s) 351, 843, 1083
MflI RGATCY 2 cut(s) 692, 1106
MhlI GDGCHC 1 cut(s) 1130
MlsI TGGCCA 1 cut(s) 233
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 2 cut(s) 158, 857
MnlI CCTC 7 cut(s) 77, 335, 350, 443, 521, 853, 903
Mox20I TGGCCA 1 cut(s) 233
MscI TGGCCA 1 cut(s) 233
MseI TTAA 8 cut(s) 8, 17, 33, 305, 333, 752, 807, 1044
MslI CAYNNNNRTG 2 cut(s) 245, 905
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 446
MunI CAATTG 3 cut(s) 351, 843, 1083
Mva1269I GAATGC 1 cut(s) 977
MwoI GCNNNNNNNGC 4 cut(s) 151, 785, 876, 1125
NcoI CCATGG 1 cut(s) 696
NdeII GATC 4 cut(s) 532, 692, 924, 1106
NlaIII CATG 9 cut(s) 340, 411, 524, 599, 628, 700, 890, 907, 976
NlaIV GGNNCC 2 cut(s) 694, 954
NmuCI GTSAC 1 cut(s) 745
PagI TCATGA 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 410
PctI GAATGC 1 cut(s) 977
PkrI GCNGC 2 cut(s) 144, 208
PleI GAGTC 2 cut(s) 158, 857
PpsI GAGTC 2 cut(s) 158, 857
PshAI GACNNNNGTC 1 cut(s) 198
PsiI TTATAA 1 cut(s) 833
PspN4I GGNNCC 2 cut(s) 694, 954
PsuI RGATCY 2 cut(s) 692, 1106
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 2 cut(s) 245, 905
SapI GCTCTTC 1 cut(s) 410
SaqAI TTAA 8 cut(s) 8, 17, 33, 305, 333, 752, 807, 1044
SatI GCNGC 2 cut(s) 143, 207
Sau3AI GATC 4 cut(s) 532, 692, 924, 1106
SchI GAGTC 2 cut(s) 158, 857
SduI GDGCHC 1 cut(s) 1130
SfaNI GCATC 1 cut(s) 909
SfcI CTRYAG 2 cut(s) 149, 855
SmiMI CAYNNNNRTG 2 cut(s) 245, 905
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SsiI CCGC 1 cut(s) 173
SspI AATATT 1 cut(s) 731
SspMI CTAG 1 cut(s) 290
StyI CCWWGG 2 cut(s) 648, 696
TaaI ACNGT 4 cut(s) 590, 884, 934, 1006
TaiI ACGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 113, 464, 1071
TatI WGTACW 1 cut(s) 763
Tru1I TTAA 8 cut(s) 8, 17, 33, 305, 333, 752, 807, 1044
Tru9I TTAA 8 cut(s) 8, 17, 33, 305, 333, 752, 807, 1044
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 2 cut(s) 142, 206
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 5 cut(s) 509, 537, 641, 658, 784
TspRI CASTG 1 cut(s) 388
XapI RAATTY 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 134
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.