MD16G1072500.v1.1

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009601
Physical Location & Seq
Reverse (-)
5081123 .. 5083287
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1072500.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGACTGCCTTAAAGCAAAGAAAATTGAGAGCCAAAACCTACCCTCACAGGAACCAACCAATTGTGGAGGCACAACATTTTGCAGAACATGTTTCAATGGTCTCAACACATTATCAGGAGTTGGGATTCTATCAATTCCATTTGCACTTTCTGAAGGAGGGTGGCTGAGCTTGATCCTTCTTTTCCAGTTGGCGCTTCTTTGTTGGTATACCGGGTTGCTTCTACAACGATGCATGGACGGGAATCCGCGGATCAAGAGTTATCCCGATATTGGAGAAGTTGCTTATGGACAGAAAGGAAGACTCACCATCTCCATTTTTATGTACCTTGAGCTGTATTTGGTAGCTGTTGAGTTTCTAATACTAGAAGGTGACAACTTAAACCAGTTGTTCCCAAGCATGGGGTTCAACATGGCAGGCTTCAAAGTTGGAGGCAAACAATGCTTTGTTTTGCTCACTGCCCTTGTAACTCTGCCAACTACATGGCTGAAGAATCTGGGATTGTTGGCGTATGTTTCTGCCGGAGGGGTTTTTGCTTCTCTTCTTTTGGTTGCTTTCGTGTTTTGGGTTGGCACAGTTGATGGTGTAGGGTTTCATGAAGGAGATGTTCTTCTGAATTTCAAAGGATTGCCTACTGCTGTGAGCTTGTACCTGTTTTGCTATTGTGGCCATGCAGTTTTCCCCACATTGTGCAATTCCATGAAAGATAGAAGCAAATTCTCCAAGGTGTTACTTTTCTGCTTCGTTGTAAGCACCATCACCTACGGATCAATGGCGGTTTTGGGTTACTTGATGTTCGGACAAGACTTGAAGTCTCAAGTCACATTAAATCTTCCAATACGAAAAATTAGTTCAAGGATAGCAATTTACATTACTATAATTAATCCCCTCACAAAGTATGCAATTATAATCACTCCAATTGCTGCTGCTATTGAGGATACACGCCCTTTTCGCAACAGCCTAACTATTAGCATCTTCGTGCGAACCTTAATTGTAATTAGCACCGTAATTGTGGCATTATCCATTCCTTTTTTTAGCTACCTAATGGCTTTTGTCGGCGCATCTTTGAGCGTGACGGTCTCAGTCTTGCTGCCGTGCTTGTTCTACCTTAAGATTAACGTTGCTGCTCGGAGATTTGGGTTCGAGTTGGTGGTAATTGTTGGGATTATGGTTATAGGGTCGTTTGTTGGCGTAGTGGGTACGTACACTTCTGTGATACAAATTGTAAGACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

413

Amino Acids

45.12

Weight (kDa)

9.01

Isoelectric Point (pI)

23.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 24 - 402 4e-64 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
AccI GTMKAC 1 cut(s) 209
AccII CGCG 1 cut(s) 250
AciI CCGC 3 cut(s) 248, 250, 776
AclI AACGTT 1 cut(s) 1119
AclWI GGATC 3 cut(s) 169, 260, 775
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 2 cut(s) 616, 716
AcuI CTGAAG 2 cut(s) 174, 509
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 4 cut(s) 326, 650, 1201, 1205
AfiI CCNNNNNNNGG 3 cut(s) 272, 400, 401
AflII CTTAAG 1 cut(s) 1109
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 6 cut(s) 97, 409, 424, 622, 811, 855
AhdI GACNNNNNGTC 1 cut(s) 811
AleI CACNNNNGTG 1 cut(s) 1211
AluBI AGCT 5 cut(s) 171, 334, 347, 645, 1038
AluI AGCT 5 cut(s) 171, 334, 347, 645, 1038
Alw26I GTCTC 3 cut(s) 107, 819, 1084
AlwI GGATC 3 cut(s) 169, 260, 775
AoxI GGCC 1 cut(s) 667
ApeKI GCWGC 4 cut(s) 923, 926, 1090, 1124
ApoI RAATTY 2 cut(s) 616, 716
AseI ATTAAT 1 cut(s) 882
AspLEI GCGC 2 cut(s) 196, 1061
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 3 cut(s) 298, 383, 751
BalI TGGCCA 1 cut(s) 669
BarI GAAGNNNNNNTAC 2 cut(s) 1192, 1224
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 4 cut(s) 910, 913, 1077, 1111
BccI CCATC 3 cut(s) 317, 575, 764
BceAI ACGGC 1 cut(s) 1078
BciVI GTATCC 1 cut(s) 931
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 3 cut(s) 107, 819, 1084
BfaI CTAG 1 cut(s) 365
BfoI RGCGCY 1 cut(s) 197
BfrI CTTAAG 1 cut(s) 1109
BfuI GTATCC 1 cut(s) 931
BisI GCNGC 4 cut(s) 924, 927, 1091, 1125
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 4 cut(s) 925, 928, 1092, 1126
Bme1390I CCNGG 1 cut(s) 214
BmeRI GACNNNNNGTC 1 cut(s) 811
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 3 cut(s) 221, 981, 1070
BpiI GAAGAC 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 167
BpuEI CTTGAG 2 cut(s) 350, 801
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 1203
BsaI GGTCTC 2 cut(s) 107, 1084
BsaJI CCNNGG 2 cut(s) 248, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 400, 401
Bse1I ACTGG 2 cut(s) 187, 385
BseDI CCNNGG 2 cut(s) 248, 723
BseLI CCNNNNNNNGG 3 cut(s) 272, 400, 401
BseMII CTCAG 2 cut(s) 158, 1095
BseNI ACTGG 2 cut(s) 187, 385
BseXI GCAGC 4 cut(s) 910, 913, 1077, 1111
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 1 cut(s) 669
BsiSI CCGG 2 cut(s) 213, 522
BslI CCNNNNNNNGG 3 cut(s) 272, 400, 401
BsmAI GTCTC 3 cut(s) 107, 819, 1084
BsnI GGCC 1 cut(s) 669
Bso31I GGTCTC 2 cut(s) 107, 1084
Bsp143I GATC 3 cut(s) 174, 252, 767
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 3 cut(s) 248, 250, 776
BspANI GGCC 1 cut(s) 669
BspCNI CTCAG 2 cut(s) 159, 1094
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 3 cut(s) 169, 260, 775
BspTI CTTAAG 1 cut(s) 1109
BspTNI GGTCTC 2 cut(s) 107, 1084
BsrI ACTGG 2 cut(s) 187, 385
BssECI CCNNGG 2 cut(s) 248, 723
BssMI GATC 3 cut(s) 174, 252, 767
BssNAI GTATAC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 723
Bst1107I GTATAC 1 cut(s) 210
Bst4CI ACNGT 3 cut(s) 577, 1006, 1078
Bst6I CTCTTC 1 cut(s) 546
BstAFI CTTAAG 1 cut(s) 1109
BstAPI GCANNNNNTGC 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 1203
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 2 cut(s) 167, 1081
BstDSI CCRYGG 1 cut(s) 248
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 2 cut(s) 196, 1061
BstKTI GATC 3 cut(s) 177, 255, 770
BstMAI GTCTC 3 cut(s) 107, 819, 1084
BstMBI GATC 3 cut(s) 174, 252, 767
BstMWI GCNNNNNNNGC 3 cut(s) 441, 666, 951
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 212
BstSNI TACGTA 1 cut(s) 1203
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 4 cut(s) 910, 913, 1077, 1111
BstV2I GAAGAC 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 483
BstZ17I GTATAC 1 cut(s) 210
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 1 cut(s) 669
BtgI CCRYGG 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 418
CciI TCATGA 1 cut(s) 595
CfoI GCGC 2 cut(s) 196, 1061
Cfr42I CCGCGG 1 cut(s) 251
Csp6I GTAC 4 cut(s) 325, 649, 1200, 1204
CviAII CATG 8 cut(s) 90, 235, 400, 412, 483, 596, 671, 700
CviQI GTAC 4 cut(s) 325, 649, 1200, 1204
DdeI CTNAG 2 cut(s) 167, 1081
DpnI GATC 3 cut(s) 176, 254, 769
DpnII GATC 3 cut(s) 174, 252, 767
DraIII CACNNNGTG 1 cut(s) 690
DriI GACNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 1 cut(s) 546
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 546
Eco105I TACGTA 1 cut(s) 1203
Eco130I CCWWGG 1 cut(s) 723
Eco31I GGTCTC 2 cut(s) 107, 1084
Eco57I CTGAAG 2 cut(s) 174, 509
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
FatI CATG 8 cut(s) 89, 234, 399, 411, 482, 595, 670, 699
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 4 cut(s) 924, 927, 1091, 1125
Fsp4HI GCNGC 4 cut(s) 924, 927, 1091, 1125
FspBI CTAG 1 cut(s) 365
GlaI GCGC 2 cut(s) 195, 1060
GluI GCNGC 4 cut(s) 924, 927, 1091, 1125
HaeII RGCGCY 1 cut(s) 197
HaeIII GGCC 1 cut(s) 669
HapII CCGG 2 cut(s) 213, 522
HhaI GCGC 2 cut(s) 196, 1061
Hin1II CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
Hin6I GCGC 2 cut(s) 194, 1059
HinP1I GCGC 2 cut(s) 194, 1059
HinfI GANTC 4 cut(s) 127, 244, 303, 493
HpaII CCGG 2 cut(s) 213, 522
HphI GGTGA 3 cut(s) 298, 383, 751
Hpy166II GTNNAC 2 cut(s) 210, 1206
Hpy188I TCNGA 5 cut(s) 154, 615, 800, 1131, 1238
Hpy188III TCNNGA 4 cut(s) 117, 256, 266, 596
Hpy8I GTNNAC 2 cut(s) 210, 1206
HpyAV CCTTC 4 cut(s) 149, 188, 362, 593
HpyCH4III ACNGT 3 cut(s) 577, 1006, 1078
HpyCH4IV ACGT 2 cut(s) 1119, 1202
HpyCH4V TGCA 6 cut(s) 84, 146, 234, 674, 693, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 441, 666, 951
HpyF3I CTNAG 2 cut(s) 167, 1081
HpySE526I ACGT 2 cut(s) 1119, 1202
Hsp92II CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
HspAI GCGC 2 cut(s) 194, 1059
KspI CCGCGG 1 cut(s) 251
Kzo9I GATC 3 cut(s) 174, 252, 767
LpnPI CCDG 9 cut(s) 35, 102, 200, 226, 398, 402, 482, 535, 665
Lsp1109I GCAGC 4 cut(s) 910, 913, 1077, 1111
LweI GCATC 3 cut(s) 221, 981, 1070
MaeI CTAG 1 cut(s) 365
MaeII ACGT 2 cut(s) 1119, 1202
MaeIII GTNAC 6 cut(s) 371, 466, 729, 785, 820, 1072
MalI GATC 3 cut(s) 176, 254, 769
MboI GATC 3 cut(s) 174, 252, 767
MboII GAAGA 6 cut(s) 312, 502, 533, 602, 824, 967
MfeI CAATTG 2 cut(s) 61, 918
MlsI TGGCCA 1 cut(s) 669
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 7 cut(s) 55, 62, 152, 425, 518, 899, 928
Mox20I TGGCCA 1 cut(s) 669
Mph1103I ATGCAT 1 cut(s) 236
MscI TGGCCA 1 cut(s) 669
MseI TTAA 7 cut(s) 12, 380, 827, 882, 989, 1110, 1116
MslI CAYNNNNRTG 3 cut(s) 320, 977, 1211
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 250
MspCI CTTAAG 1 cut(s) 1109
MspI CCGG 2 cut(s) 213, 522
MspR9I CCNGG 1 cut(s) 214
MunI CAATTG 2 cut(s) 61, 918
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 3 cut(s) 441, 666, 951
NciI CCSGG 1 cut(s) 214
NdeII GATC 3 cut(s) 174, 252, 767
NlaIII CATG 8 cut(s) 93, 238, 403, 415, 486, 599, 674, 703
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 3 cut(s) 371, 820, 1072
NsiI ATGCAT 1 cut(s) 236
NspI RCATGY 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 595
PciI ACATGT 1 cut(s) 89
PfeI GAWTC 3 cut(s) 127, 244, 493
PkrI GCNGC 4 cut(s) 925, 928, 1092, 1126
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Ppu21I YACGTR 1 cut(s) 1203
PscI ACATGT 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 882
PsiI TTATAA 1 cut(s) 908
Psp1406I AACGTT 1 cut(s) 1119
PspN4I GGNNCC 1 cut(s) 54
RsaI GTAC 4 cut(s) 326, 650, 1201, 1205
RsaNI GTAC 4 cut(s) 325, 649, 1200, 1204
RseI CAYNNNNRTG 3 cut(s) 320, 977, 1211
SacII CCGCGG 1 cut(s) 251
SaqAI TTAA 7 cut(s) 12, 380, 827, 882, 989, 1110, 1116
SatI GCNGC 4 cut(s) 924, 927, 1091, 1125
Sau3AI GATC 3 cut(s) 174, 252, 767
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 1 cut(s) 214
SfaNI GCATC 3 cut(s) 221, 981, 1070
Sfr303I CCGCGG 1 cut(s) 251
SgrBI CCGCGG 1 cut(s) 251
SmiMI CAYNNNNRTG 3 cut(s) 320, 977, 1211
SmlI CTYRAG 3 cut(s) 329, 816, 1109
SmoI CTYRAG 3 cut(s) 329, 816, 1109
SnaBI TACGTA 1 cut(s) 1203
SsiI CCGC 3 cut(s) 248, 250, 776
SspMI CTAG 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 3 cut(s) 577, 1006, 1078
TaiI ACGT 2 cut(s) 1122, 1205
TaqI TCGA 1 cut(s) 1143
TfiI GAWTC 3 cut(s) 127, 244, 493
Tru1I TTAA 7 cut(s) 12, 380, 827, 882, 989, 1110, 1116
Tru9I TTAA 7 cut(s) 12, 380, 827, 882, 989, 1110, 1116
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 3 cut(s) 371, 820, 1072
TseI GCWGC 4 cut(s) 923, 926, 1090, 1124
Tsp45I GTSAC 3 cut(s) 371, 820, 1072
TspDTI ATGAA 3 cut(s) 584, 612, 716
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 1 cut(s) 463
Vha464I CTTAAG 1 cut(s) 1109
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 2 cut(s) 616, 716
XceI RCATGY 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 209
XspI CTAG 1 cut(s) 365
Zsp2I ATGCAT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.