RLG00000006237

Vacuolar amino acid transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009601
Physical Location & Seq
Forward (+)
5457319 .. 5460652
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006237

Sequence Viewer

Length: 1914 bp
ATGGAGTTGGACTCCCTCAAAGTAGAGATAGAGAGCCAAATTCAACCACTTGAACCAAACAAAGGCACCACCTTTTTCAGAACCTGTTTCAATGGACTCAACACACTATCAGGTGTGGGAATACTATCAATTCCATTTGCTCTATCTCAGGGAGGGTGGTTAAGCTTAGTGCTTCTCTTCTCGGTAGCAATCCTATGTTGGTATACAGGGCTGCTTCTACAGCGATGTATGGACTTAAATCCGCACATAAAAACTTATCCCGACATTGGTCAAGTTGCTTTTGGACGCAAAGGAAGAGTGGCCATATCCATCTTTATGTATCTTGAGCTGTATTTAGTTGCAGTTGAATTTCTAATACTAGAAGGTGATAACTTAAACCAGTTGTTTCCAAACGTGAGTTTTAACATGGGAGGCCACACAATTGGAGGTAAGCAATGCTTTGTTTTAGCCACTGCCCTTGTAATACTGCCAACTACATGGTTGAAGAGCTTGGGATTGCTGGCATATATTTCTGCTGGAGGGGTTTTGGCTTCGATTATTTTGGTTGCTTGTGTTTTTTGGGTTGGTGCTGTTGATAGTGTGGGATTTCATGAAAGAGGTGATCTCCTGAATTGGAAAGGATTGCCTACTGCTATTAGCTTATATTTGTTCTGTTACTGTGGTCATGCAGTTTTTCCCACATTATGCAATTCCATGAAAGATAGAAGCCAATTCCCCAAGGTATTGCTTGTTTGCTTCATAACAAGCACCATCACCTATGGATCCATGGCCGTTTTGGGTTACTTGATGTTTGGAGAATATTTGAAGTCCCAAGTGACATTAAATCTTCCTGTACAAAAAATTAGTGCAAAAATAGCAATTTTCATCACAATAGTTAACCCCCTGACTAAGTATGCAATTATAATCACTCCAATTGCTACTGCTATAGAGGACTCACGCCATTTTTGCAAGAGTCATGCTATTATCAGCATCATGGTGAGGACGGGGATTGTGATCAGCACCGTATGTGTGGCATTGATGGTTCCATTTTTCGGCTATGTCATGGCACTCGTCGGTGCTTTTCTGAGTGTAACTGTCTCAATATTGTTGCCGTGCCTGTTCTACCTGAAGATTAACAATGTTGCTCGTAGGTTTGGGTTCGAGTTGATGACAATTGTTGGGATTATGGTGATGGGATCTTTTGTTGGCGTAGTGGGCACTTACACTTCTCTGAAAGAAATTTGTGCTTCTCCACAATTACGGATTGATCAGTTCACAGAGGATCGGCTTTTGTACTTTGGATTTGGACTGGACATTGTTACTGTTGTTGCTATGGACAGTGTTGATAGTAGGATGCCAGTGTCTTGTTGTGATCCTGTTGTTAAGTCAAAACGAGCAGAATCAAAGATTTTCAGTACTAGCATTGATGATCTCCCCGAAACAGCATTGGTGGAAATCCTTTGTCGTTTGCCGAGTTGCAAATATGTTGTTCAATGCAAGTGTGTTTCGAAACGTTGGTGCAATCTTTTGTCGGATCCTTATTTGATTGGTCGCTTTCTGTGGCTCCAATTGGATCAGATGCCCCCAATTGTAACTCGTAGTACTCCCATAGTAAACAGCAATTTCGGGCTTGAACTCTTCACTGCTGAGGTGTTTGAGAAACTACGGAGTTTGTTCAGTTTGAAAAGTAATCCACTAGTGCTAGCAACATGGAATGACTTAGCTCTGTGCTGTTCATTTCGTTCTGATTACCTCATTTACTTCATCTGCAATCCGCGCACCATGCAACTGGTCACTCTTCCTCCTGTCCCTCGGTACCACAGTAGTCAGCCTGTGGGATTCATCTGTGAGCCCTACTACTATAAGGAAGACAGAAGTGGCATTGATCAGCAAAGCGAAAGGAAGGTTCAAGGTTGTGAGAATACTTCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

638

Amino Acids

70.78

Weight (kDa)

8.19

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 22 - 401 1.5e-67 Transmembrane amino acid transporter protein
F-box PF00646 468 - 508 5.2e-06 F-box domain
F-box-like PF12937 468 - 508 9.3e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
Acc65I GGTACC 1 cut(s) 1794
AccB1I GGYRCC 2 cut(s) 65, 1794
AccI GTMKAC 1 cut(s) 203
AccII CGCG 1 cut(s) 1756
AciI CCGC 2 cut(s) 242, 1754
AclI AACGTT 1 cut(s) 1492
AclWI GGATC 8 cut(s) 756, 769, 1183, 1269, 1346, 1508, 1521, 1560
AcoI YGGCCR 2 cut(s) 300, 768
AcsI RAATTY 3 cut(s) 39, 347, 1218
AcuI CTGAAG 1 cut(s) 1127
AfaI GTAC 5 cut(s) 834, 1274, 1396, 1582, 1796
AfiI CCNNNNNNNGG 3 cut(s) 62, 266, 1031
AhlI ACTAGT 1 cut(s) 1675
AluBI AGCT 5 cut(s) 165, 328, 489, 639, 1703
AluI AGCT 5 cut(s) 165, 328, 489, 639, 1703
Alw26I GTCTC 1 cut(s) 1081
AlwI GGATC 8 cut(s) 756, 769, 1183, 1269, 1346, 1508, 1521, 1560
AlwNI CAGNNNCTG 1 cut(s) 84
AoxI GGCC 3 cut(s) 300, 412, 768
ApeKI GCWGC 1 cut(s) 211
ApoI RAATTY 3 cut(s) 39, 347, 1218
Asp718I GGTACC 1 cut(s) 1794
AspLEI GCGC 1 cut(s) 1758
AsuHPI GGTGA 5 cut(s) 377, 611, 745, 988, 1180
AsuII TTCGAA 1 cut(s) 1487
AsuNHI GCTAGC 1 cut(s) 1681
BaeGI GKGCMC 1 cut(s) 1199
BaeI ACNNNNGTAYC 2 cut(s) 1786, 1819
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 2 cut(s) 761, 1513
BanI GGYRCC 2 cut(s) 65, 1794
BanII GRGCYC 1 cut(s) 1833
BbsI GAAGAC 1 cut(s) 1854
BbvCI CCTCAGC 1 cut(s) 1626
BbvI GCAGC 1 cut(s) 198
BccI CCATC 4 cut(s) 317, 758, 1012, 1165
BceAI ACGGC 2 cut(s) 755, 1075
BclI TGATCA 3 cut(s) 993, 1246, 1864
BcoDI GTCTC 1 cut(s) 1081
BcuI ACTAGT 1 cut(s) 1675
BfaI CTAG 4 cut(s) 359, 1398, 1676, 1682
BfmI CTRYAG 2 cut(s) 218, 924
BisI GCNGC 1 cut(s) 212
BlsI GCNGC 1 cut(s) 213
BmcAI AGTACT 2 cut(s) 1396, 1582
BmiI GGNNCC 6 cut(s) 67, 763, 1023, 1515, 1544, 1796
BmsI GCATC 3 cut(s) 978, 1323, 1548
BmtI GCTAGC 1 cut(s) 1685
BoxI GACNNNNGTC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 1854
BplI GAGNNNNNCTC 3 cut(s) 28, 588, 620
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 1626
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 1 cut(s) 344
BsaBI GATNNNNATC 1 cut(s) 992
BsaJI CCNNGG 3 cut(s) 717, 765, 1790
BsaXI ACNNNNNCTCC 2 cut(s) 1765, 1795
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 266, 1031
Bse1I ACTGG 4 cut(s) 379, 1293, 1337, 1773
Bse3DI GCAATG 1 cut(s) 440
Bse8I GATNNNNATC 1 cut(s) 992
BseDI CCNNGG 3 cut(s) 717, 765, 1790
BseGI GGATG 1 cut(s) 1338
BseJI GATNNNNATC 1 cut(s) 992
BseLI CCNNNNNNNGG 3 cut(s) 62, 266, 1031
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 3 cut(s) 161, 1055, 1617
BseNI ACTGG 4 cut(s) 379, 1293, 1337, 1773
BseSI GKGCMC 1 cut(s) 1199
BseXI GCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 1756
BshFI GGCC 3 cut(s) 302, 414, 770
BshNI GGYRCC 2 cut(s) 65, 1794
BslFI GGGAC 2 cut(s) 793, 1772
BslI CCNNNNNNNGG 3 cut(s) 62, 266, 1031
BsmAI GTCTC 1 cut(s) 1081
BsmFI GGGAC 2 cut(s) 793, 1772
BsnI GGCC 3 cut(s) 302, 414, 770
Bsp119I TTCGAA 1 cut(s) 1487
Bsp1286I GDGCHC 2 cut(s) 1199, 1833
Bsp1407I TGTACA 1 cut(s) 832
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 2 cut(s) 242, 1754
BspANI GGCC 3 cut(s) 302, 414, 770
BspCNI CTCAG 3 cut(s) 160, 1056, 1618
BspFNI CGCG 1 cut(s) 1756
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 6 cut(s) 67, 763, 1023, 1515, 1544, 1796
BspOI GCTAGC 1 cut(s) 1685
BspPI GGATC 8 cut(s) 756, 769, 1183, 1269, 1346, 1508, 1521, 1560
BspQI GCTCTTC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 2 cut(s) 65, 1794
BsrDI GCAATG 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 832
BsrI ACTGG 4 cut(s) 379, 1293, 1337, 1773
BssECI CCNNGG 3 cut(s) 717, 765, 1790
BssNAI GTATAC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 717, 765
Bst1107I GTATAC 1 cut(s) 204
Bst4CI ACNGT 6 cut(s) 659, 1003, 1075, 1303, 1319, 1802
Bst6I CTCTTC 5 cut(s) 182, 289, 479, 1622, 1782
BstAUI TGTACA 1 cut(s) 832
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 2 cut(s) 501, 1683
BstDEI CTNAG 6 cut(s) 147, 166, 888, 1064, 1626, 1699
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 1 cut(s) 1338
BstFNI CGCG 1 cut(s) 1756
BstHHI GCGC 1 cut(s) 1758
BstMAI GTCTC 1 cut(s) 1081
BstMWI GCNNNNNNNGC 6 cut(s) 220, 854, 945, 1194, 1755, 1762
BstPAI GACNNNNGTC 1 cut(s) 267
BstSFI CTRYAG 2 cut(s) 218, 924
BstSLI GKGCMC 1 cut(s) 1199
BstUI CGCG 1 cut(s) 1756
BstV1I GCAGC 1 cut(s) 198
BstV2I GAAGAC 1 cut(s) 1854
BstX2I RGATCY 3 cut(s) 761, 1175, 1513
BstXI CCANNNNNNTGG 3 cut(s) 422, 477, 1768
BstYI RGATCY 3 cut(s) 761, 1175, 1513
BstZ17I GTATAC 1 cut(s) 204
BsuRI GGCC 3 cut(s) 302, 414, 770
BtgI CCRYGG 1 cut(s) 765
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 1338
BtsI GCAGTG 2 cut(s) 450, 1620
BtsIMutI CAGTG 4 cut(s) 450, 1324, 1344, 1620
Cac8I GCNNGC 2 cut(s) 501, 1683
CaiI CAGNNNCTG 1 cut(s) 84
CciI TCATGA 1 cut(s) 589
CfoI GCGC 1 cut(s) 1758
CseI GACGC 1 cut(s) 294
Csp6I GTAC 5 cut(s) 833, 1273, 1395, 1581, 1795
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 5 cut(s) 833, 1273, 1395, 1581, 1795
DdeI CTNAG 6 cut(s) 147, 166, 888, 1064, 1626, 1699
EaeI YGGCCR 2 cut(s) 300, 768
Eam1104I CTCTTC 5 cut(s) 182, 289, 479, 1622, 1782
EarI CTCTTC 5 cut(s) 182, 289, 479, 1622, 1782
Eco130I CCWWGG 2 cut(s) 717, 765
Eco24I GRGCYC 1 cut(s) 1833
Eco57I CTGAAG 1 cut(s) 1127
EcoT14I CCWWGG 2 cut(s) 717, 765
EcoT38I GRGCYC 1 cut(s) 1833
ErhI CCWWGG 2 cut(s) 717, 765
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 2 cut(s) 793, 1772
FbaI TGATCA 3 cut(s) 993, 1246, 1864
FblI GTMKAC 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 1345
FriOI GRGCYC 1 cut(s) 1833
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 4 cut(s) 359, 1398, 1676, 1682
GlaI GCGC 1 cut(s) 1757
GluI GCNGC 1 cut(s) 212
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 3 cut(s) 302, 414, 770
HgaI GACGC 1 cut(s) 294
HhaI GCGC 1 cut(s) 1758
Hin6I GCGC 1 cut(s) 1756
HinP1I GCGC 1 cut(s) 1756
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 6 cut(s) 11, 96, 932, 952, 1379, 1818
HpaI GTTAAC 1 cut(s) 877
HphI GGTGA 5 cut(s) 377, 611, 745, 988, 1180
Hpy166II GTNNAC 4 cut(s) 204, 877, 1254, 1594
Hpy188I TCNGA 6 cut(s) 80, 1065, 1212, 1513, 1557, 1726
Hpy188III TCNNGA 5 cut(s) 260, 323, 590, 607, 1911
Hpy8I GTNNAC 4 cut(s) 204, 877, 1254, 1594
Hpy99I CGWCG 1 cut(s) 1055
HpyAV CCTTC 2 cut(s) 356, 1876
HpyCH4III ACNGT 6 cut(s) 659, 1003, 1075, 1303, 1319, 1802
HpyCH4IV ACGT 2 cut(s) 393, 1492
HpyF10VI GCNNNNNNNGC 6 cut(s) 220, 854, 945, 1194, 1755, 1762
HpyF3I CTNAG 6 cut(s) 147, 166, 888, 1064, 1626, 1699
HpySE526I ACGT 2 cut(s) 393, 1492
HspAI GCGC 1 cut(s) 1756
KpnI GGTACC 1 cut(s) 1798
Ksp22I TGATCA 3 cut(s) 993, 1246, 1864
KspAI GTTAAC 1 cut(s) 877
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 1 cut(s) 1548
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 978, 1323, 1548
MaeI CTAG 4 cut(s) 359, 1398, 1676, 1682
MaeII ACGT 2 cut(s) 393, 1492
MaeIII GTNAC 7 cut(s) 653, 779, 814, 1069, 1297, 1570, 1771
MboII GAAGA 8 cut(s) 169, 306, 496, 818, 1120, 1609, 1769, 1859
MfeI CAATTG 5 cut(s) 420, 912, 1152, 1547, 1566
MflI RGATCY 3 cut(s) 761, 1175, 1513
MhlI GDGCHC 2 cut(s) 1199, 1833
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 4 cut(s) 5, 90, 926, 961
MmeI TCCRAC 1 cut(s) 1491
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 8 cut(s) 161, 236, 374, 402, 821, 876, 1113, 1362
MslI CAYNNNNRTG 2 cut(s) 314, 974
Msp20I TGGCCA 1 cut(s) 302
MunI CAATTG 5 cut(s) 420, 912, 1152, 1547, 1566
MvnI CGCG 1 cut(s) 1756
MwoI GCNNNNNNNGC 6 cut(s) 220, 854, 945, 1194, 1755, 1762
NcoI CCATGG 1 cut(s) 765
NheI GCTAGC 1 cut(s) 1681
NlaIV GGNNCC 6 cut(s) 67, 763, 1023, 1515, 1544, 1796
NmeAIII GCCGAG 1 cut(s) 1476
NmuCI GTSAC 2 cut(s) 814, 1771
NspV TTCGAA 1 cut(s) 1487
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 479
PfeI GAWTC 2 cut(s) 1379, 1818
PkrI GCNGC 1 cut(s) 213
PleI GAGTC 4 cut(s) 5, 90, 926, 960
PpsI GAGTC 4 cut(s) 5, 90, 926, 960
PshAI GACNNNNGTC 1 cut(s) 267
PsiI TTATAA 1 cut(s) 902
Psp1406I AACGTT 1 cut(s) 1492
PspN4I GGNNCC 6 cut(s) 67, 763, 1023, 1515, 1544, 1796
PstNI CAGNNNCTG 1 cut(s) 84
PsuI RGATCY 3 cut(s) 761, 1175, 1513
RsaI GTAC 5 cut(s) 834, 1274, 1396, 1582, 1796
RsaNI GTAC 5 cut(s) 833, 1273, 1395, 1581, 1795
RseI CAYNNNNRTG 2 cut(s) 314, 974
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 8 cut(s) 161, 236, 374, 402, 821, 876, 1113, 1362
SatI GCNGC 1 cut(s) 212
ScaI AGTACT 2 cut(s) 1396, 1582
SchI GAGTC 4 cut(s) 5, 90, 926, 961
SduI GDGCHC 2 cut(s) 1199, 1833
SfaNI GCATC 3 cut(s) 978, 1323, 1548
SfcI CTRYAG 2 cut(s) 218, 924
SfuI TTCGAA 1 cut(s) 1487
SmiMI CAYNNNNRTG 2 cut(s) 314, 974
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SpeI ACTAGT 1 cut(s) 1675
SsiI CCGC 2 cut(s) 242, 1754
SspI AATATT 2 cut(s) 800, 1083
SspMI CTAG 4 cut(s) 359, 1398, 1676, 1682
StyI CCWWGG 2 cut(s) 717, 765
TaaI ACNGT 6 cut(s) 659, 1003, 1075, 1303, 1319, 1802
TaiI ACGT 2 cut(s) 396, 1495
TaqI TCGA 3 cut(s) 533, 1140, 1487
TatI WGTACW 4 cut(s) 832, 1272, 1394, 1580
TfiI GAWTC 2 cut(s) 1379, 1818
Tru1I TTAA 8 cut(s) 161, 236, 374, 402, 821, 876, 1113, 1362
Tru9I TTAA 8 cut(s) 161, 236, 374, 402, 821, 876, 1113, 1362
TscAI CASTG 4 cut(s) 457, 1324, 1344, 1627
TseFI GTSAC 2 cut(s) 814, 1771
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 2 cut(s) 814, 1771
TspDTI ATGAA 8 cut(s) 578, 606, 710, 727, 853, 1704, 1732, 1810
TspGWI ACGGA 2 cut(s) 1255, 1660
TspRI CASTG 4 cut(s) 457, 1324, 1344, 1627
XapI RAATTY 3 cut(s) 39, 347, 1218
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 203
XspI CTAG 4 cut(s) 359, 1398, 1676, 1682
ZrmI AGTACT 2 cut(s) 1396, 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.