Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011725

Physical Location & Seq

Genomic Coordinates Reverse (-)

5511977 .. 5514284

Gene Structure 2308 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1078400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGCAGCAATGTTTAACTCGTCCTATTTCTTTCTATGTTTGTTTTTCTTATCCTTTGCTGTATTTGTTTCGAGTAATGATGGTCCAACCGATGTTGCAAATGAAGATAGTACGGTATTAGATTTAGATGCGTACTGGAAAGAACGAGCCCAAGAAGCCTTAAAGGAGGCCATGGAAAGCTACCATAAAAATCCTGAGCAAGTCACTGAGGAGTTCAATACTGGAGTTGCCGAGCTTTTGTCCAAGGAAAACACCACAAGAAGGAACTTGAGGGGTAACAAGGGTTATAATGGTCCATGCATGGCGACCAATCCAATTGACGCCTGCTGGAGGTGTGACCCTAATTGGGCCGATAACCGAAAGAAGATTGTAGGCTGCGCCCAAGGCTTTGGTAAAAAGGCCACTGGAGGTAAGGACGGTCCCATTTACGTGGTGACTGTGGGAACAGATGATGACGTACAAAACCCCAAACCCGGAACCCTACGCCACGCCGTGATTCAGAAAGGGCCTTTGTGGATAATCTTCGCAAGAAGCATGGTGATTAGGCTGCAGCAAGAGCTGATGGTGACCAGTGACAAGACCATTGATGGTAGAGGAGCCAATGTTGTGATAGAAGAGGGTGGTGGCATTACCCTCCAGTTTGTGAAGAATGTAATCCTCACCAACCTTCACATCAAAATGATTGTGTCCAAACCCGGTGGTATGATCAGGGACTCCGTCGACCATATGGGTTTGAGGACCAAGAGTGACGGCGATGGTATCTCCCTCTTTGGTGCCTCCAACGTCTGGATCGACCACATGTCCATGTCCCGGTGCGCAGATGGGCTCATCGATGCCGTCATGGCTTCCACCGCCATCACCATCTCCAACTCCCACTTCACTGACCACGATGATGTGATGTTGTTTGGCGCCAACAACGCGCACACTCAAGACAAAATCATGCAAATTACTTTGGCCTTCAACCACTTTGGTCGGGGACTGGTGCAGAGGATGCCGAGGGTCCGGCACGGATTCTTCCACCTGGTGAACAACGACTACAACCACTGGATCATGTATGCCATCGGAGGAAACATGAACCCTACAATCATCAGCCAGGGTAACAGGTTCATTGCTCCCGTGAACGAGGCCACCAAACAGGTGACCCATAGGGAGCATACACCAGAGTCAGAGTGGAAGAACTGGGAGTGGAGATCTGAGGGAGATTTGATGATGAATGGAGCTTTCTTTGTTGAATCTGGATCAAGAGCCAGCCAAAACCCAGCAAAGTTGGACATGATGCCCTTCCAACCAGGGACCTTTGCCACAAAGCTCACAAAATTTTCCGGCGCTCTAAGCTGCGTTGTCGGCAAACCCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

453

Amino Acids

50.15

Weight (kDa)

6.54

Isoelectric Point (pI)

30.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	40 - 90	9.8e-18	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	185 - 383	2.6e-21	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14420 AT2G02720
fragaria_vesca	FvH4_4g31900
malus_domestica	MD13G1078400.v1.1 MD16G1078000.v1.1 MD16G1078100.v1.1
prunus_persica	Prupe.1G268500_v2.0.a1 Prupe.1G268700_v2.0.a1
pyrus_communis	pycom13g06810 pycom16g06740 pycom16g06760
rosa_chinensis	RchiOBHm_Chr4g0440551
rosa_rugosa	Rorug04G0326600
rosa_samantha	Rh4AG378300 Rh4BG391000 Rh4CG405700 Rh4DG384700
rosa_wichuraiana	Rw4G032390 Rw4G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	288
Acc16I	TGCGCA	1 cut(s)	817
AccB1I	GGYRCC	2 cut(s)	773, 908
AccB7I	CCANNNNNTGG	1 cut(s)	786
AccI	GTMKAC	1 cut(s)	720
AccII	CGCG	1 cut(s)	920
AciI	CCGC	1 cut(s)	852
AclWI	GGATC	3 cut(s)	797, 1055, 1246
AcsI	RAATTY	1 cut(s)	1316
AcyI	GRCGYC	2 cut(s)	321, 909
AdeI	CACNNNGTG	2 cut(s)	493, 1024
AfaI	GTAC	3 cut(s)	112, 134, 459
AfiI	CCNNNNNNNGG	6 cut(s)	261, 330, 346, 473, 786, 810
AflIII	ACRYGT	1 cut(s)	798
AgsI	TTSAA	3 cut(s)	217, 961, 1232
AhdI	GACNNNNNGTC	1 cut(s)	799
AjnI	CCWGG	3 cut(s)	1020, 1092, 1288
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AluBI	AGCT	6 cut(s)	180, 235, 559, 1220, 1309, 1335
AluI	AGCT	6 cut(s)	180, 235, 559, 1220, 1309, 1335
AlwI	GGATC	3 cut(s)	797, 1055, 1246
AoxI	GGCC	6 cut(s)	168, 348, 399, 506, 954, 1125
ApeKI	GCWGC	5 cut(s)	5, 375, 547, 550, 1335
ApoI	RAATTY	1 cut(s)	1316
AspLEI	GCGC	5 cut(s)	380, 818, 911, 922, 1328
AspS9I	GGNCC	8 cut(s)	83, 293, 348, 419, 506, 738, 1000, 1293
AsuC2I	CCSGG	3 cut(s)	474, 696, 811
AsuHPI	GGTGA	7 cut(s)	445, 550, 577, 652, 850, 1036, 1150
AvaII	GGWCC	6 cut(s)	83, 293, 419, 738, 1000, 1293
BanI	GGYRCC	2 cut(s)	773, 908
BanII	GRGCYC	2 cut(s)	151, 828
BbvI	GCAGC	5 cut(s)	17, 362, 534, 562, 1322
BccI	CCATC	8 cut(s)	74, 556, 581, 749, 815, 863, 869, 1067
BceAI	ACGGC	3 cut(s)	476, 766, 821
BciT130I	CCWGG	3 cut(s)	1022, 1094, 1290
BclI	TGATCA	1 cut(s)	705
BcnI	CCSGG	3 cut(s)	474, 696, 811
BfmI	CTRYAG	1 cut(s)	548
BfoI	RGCGCY	2 cut(s)	912, 1329
BglI	GCCNNNNNGGC	1 cut(s)	842
BglII	AGATCT	1 cut(s)	1190
BisI	GCNGC	5 cut(s)	6, 376, 548, 551, 1336
BlsI	GCNGC	5 cut(s)	7, 377, 549, 552, 1337
Bme1390I	CCNGG	6 cut(s)	474, 696, 811, 1022, 1094, 1290
Bme18I	GGWCC	6 cut(s)	83, 293, 419, 738, 1000, 1293
BmeRI	GACNNNNNGTC	1 cut(s)	799
BmgT120I	GGNCC	8 cut(s)	83, 293, 348, 419, 506, 738, 1000, 1293
BmiI	GGNNCC	7 cut(s)	421, 478, 598, 775, 910, 1001, 1294
BmrFI	CCNGG	6 cut(s)	474, 696, 811, 1022, 1094, 1290
BmrI	ACTGGG	1 cut(s)	1189
BmsI	GCATC	4 cut(s)	118, 823, 981, 1266
BmuI	ACTGGG	1 cut(s)	1189
BpmI	CTGGAG	4 cut(s)	243, 349, 426, 620
Bpu10I	CCTNAGC	1 cut(s)	195
BpuEI	CTTGAG	2 cut(s)	289, 912
BpuMI	CCSGG	3 cut(s)	474, 696, 811
Bsa29I	ATCGAT	1 cut(s)	831
BsaAI	YACGTR	1 cut(s)	430
BsaHI	GRCGYC	2 cut(s)	321, 909
BsaJI	CCNNGG	6 cut(s)	171, 243, 382, 995, 1093, 1289
BsaXI	ACNNNNNCTCC	2 cut(s)	588, 618
Bsc4I	CCNNNNNNNGG	6 cut(s)	261, 330, 346, 473, 786, 810
Bse1I	ACTGG	8 cut(s)	140, 226, 409, 570, 637, 984, 1049, 1184
Bse3DI	GCAATG	2 cut(s)	15, 1107
BseBI	CCWGG	3 cut(s)	1022, 1094, 1290
BseCI	ATCGAT	1 cut(s)	831
BseDI	CCNNGG	6 cut(s)	171, 243, 382, 995, 1093, 1289
BseGI	GGATG	1 cut(s)	996
BseLI	CCNNNNNNNGG	6 cut(s)	261, 330, 346, 473, 786, 810
BseMI	GCAATG	2 cut(s)	15, 1107
BseMII	CTCAG	3 cut(s)	186, 198, 1185
BseNI	ACTGG	8 cut(s)	140, 226, 409, 570, 637, 984, 1049, 1184
BseRI	GAGGAG	2 cut(s)	224, 609
BseXI	GCAGC	5 cut(s)	17, 362, 534, 562, 1322
BseYI	CCCAGC	1 cut(s)	1258
BsgI	GTGCAG	1 cut(s)	1004
Bsh1236I	CGCG	1 cut(s)	920
BshFI	GGCC	6 cut(s)	170, 350, 401, 508, 956, 1127
BshNI	GGYRCC	2 cut(s)	773, 908
BshVI	ATCGAT	1 cut(s)	831
BsiSI	CCGG	5 cut(s)	474, 696, 811, 1003, 1323
BslFI	GGGAC	5 cut(s)	405, 725, 793, 990, 1306
BslI	CCNNNNNNNGG	6 cut(s)	261, 330, 346, 473, 786, 810
BsmFI	GGGAC	5 cut(s)	405, 725, 793, 990, 1306
BsnI	GGCC	6 cut(s)	170, 350, 401, 508, 956, 1127
Bsp1286I	GDGCHC	2 cut(s)	151, 828
Bsp143I	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
Bsp19I	CCATGG	1 cut(s)	171
BspACI	CCGC	1 cut(s)	852
BspANI	GGCC	6 cut(s)	170, 350, 401, 508, 956, 1127
BspCNI	CTCAG	3 cut(s)	187, 199, 1186
BspDI	ATCGAT	1 cut(s)	831
BspFNI	CGCG	1 cut(s)	920
BspLI	GGNNCC	7 cut(s)	421, 478, 598, 775, 910, 1001, 1294
BspMAI	CTGCAG	1 cut(s)	552
BspPI	GGATC	3 cut(s)	797, 1055, 1246
BspT107I	GGYRCC	2 cut(s)	773, 908
BsrDI	GCAATG	2 cut(s)	15, 1107
BsrI	ACTGG	8 cut(s)	140, 226, 409, 570, 637, 984, 1049, 1184
BssECI	CCNNGG	6 cut(s)	171, 243, 382, 995, 1093, 1289
BssMI	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
BssNI	GRCGYC	2 cut(s)	321, 909
BssT1I	CCWWGG	3 cut(s)	171, 243, 382
Bst2UI	CCWGG	3 cut(s)	1022, 1094, 1290
Bst4CI	ACNGT	3 cut(s)	115, 419, 439
Bst6I	CTCTTC	1 cut(s)	609
BstACI	GRCGYC	2 cut(s)	321, 909
BstAPI	GCANNNNNTGC	2 cut(s)	991, 1353
BstBAI	YACGTR	1 cut(s)	430
BstC8I	GCNNGC	2 cut(s)	325, 1249
BstDEI	CTNAG	4 cut(s)	195, 207, 1194, 1331
BstDSI	CCRYGG	1 cut(s)	171
BstEII	GGTNACC	2 cut(s)	565, 1138
BstENI	CCTNNNNNAGG	1 cut(s)	328
BstF5I	GGATG	1 cut(s)	996
BstFNI	CGCG	1 cut(s)	920
BstH2I	RGCGCY	2 cut(s)	912, 1329
BstHHI	GCGC	5 cut(s)	380, 818, 911, 922, 1328
BstKTI	GATC	5 cut(s)	708, 792, 1050, 1193, 1241
BstMBI	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
BstNI	CCWGG	3 cut(s)	1022, 1094, 1290
BstNSI	RCATGY	1 cut(s)	802
BstPI	GGTNACC	2 cut(s)	565, 1138
BstSCI	CCNGG	6 cut(s)	472, 694, 809, 1020, 1092, 1288
BstSFI	CTRYAG	1 cut(s)	548
BstUI	CGCG	1 cut(s)	920
BstV1I	GCAGC	5 cut(s)	17, 362, 534, 562, 1322
BstX2I	RGATCY	1 cut(s)	1190
BstXI	CCANNNNNNTGG	2 cut(s)	389, 430
BstYI	RGATCY	1 cut(s)	1190
Bsu15I	ATCGAT	1 cut(s)	831
BsuRI	GGCC	6 cut(s)	170, 350, 401, 508, 956, 1127
BsuTUI	ATCGAT	1 cut(s)	831
BtgI	CCRYGG	1 cut(s)	171
BtgZI	GCGATG	1 cut(s)	768
BtsCI	GGATG	1 cut(s)	996
BtsIMutI	CAGTG	5 cut(s)	204, 402, 577, 879, 1042
Cac8I	GCNNGC	2 cut(s)	325, 1249
CfoI	GCGC	5 cut(s)	380, 818, 911, 922, 1328
Cfr13I	GGNCC	8 cut(s)	83, 293, 348, 419, 506, 738, 1000, 1293
ClaI	ATCGAT	1 cut(s)	831
CseI	GACGC	1 cut(s)	329
CsiI	ACCWGGT	1 cut(s)	1020
Csp6I	GTAC	3 cut(s)	111, 133, 458
CspCI	CAANNNNNGTGG	2 cut(s)	679, 714
CviQI	GTAC	3 cut(s)	111, 133, 458
DdeI	CTNAG	4 cut(s)	195, 207, 1194, 1331
DinI	GGCGCC	1 cut(s)	910
DpnI	GATC	5 cut(s)	707, 791, 1049, 1192, 1240
DpnII	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
DraIII	CACNNNGTG	2 cut(s)	493, 1024
DriI	GACNNNNNGTC	1 cut(s)	799
Eam1104I	CTCTTC	1 cut(s)	609
Eam1105I	GACNNNNNGTC	1 cut(s)	799
EarI	CTCTTC	1 cut(s)	609
Eco130I	CCWWGG	3 cut(s)	171, 243, 382
Eco24I	GRGCYC	2 cut(s)	151, 828
Eco47I	GGWCC	6 cut(s)	83, 293, 419, 738, 1000, 1293
Eco91I	GGTNACC	2 cut(s)	565, 1138
EcoNI	CCTNNNNNAGG	1 cut(s)	328
EcoO109I	RGGNCCY	2 cut(s)	506, 1293
EcoO65I	GGTNACC	2 cut(s)	565, 1138
EcoRII	CCWGG	3 cut(s)	1020, 1092, 1288
EcoT14I	CCWWGG	3 cut(s)	171, 243, 382
EcoT22I	ATGCAT	1 cut(s)	302
EcoT38I	GRGCYC	2 cut(s)	151, 828
EgeI	GGCGCC	1 cut(s)	910
EheI	GGCGCC	1 cut(s)	910
ErhI	CCWWGG	3 cut(s)	171, 243, 382
FaqI	GGGAC	5 cut(s)	405, 725, 793, 990, 1306
FauNDI	CATATG	1 cut(s)	726
FbaI	TGATCA	1 cut(s)	705
FblI	GTMKAC	1 cut(s)	720
Fnu4HI	GCNGC	5 cut(s)	6, 376, 548, 551, 1336
FokI	GGATG	1 cut(s)	1003
FriOI	GRGCYC	2 cut(s)	151, 828
Fsp4HI	GCNGC	5 cut(s)	6, 376, 548, 551, 1336
FspI	TGCGCA	1 cut(s)	817
GlaI	GCGC	5 cut(s)	379, 817, 910, 921, 1327
GluI	GCNGC	5 cut(s)	6, 376, 548, 551, 1336
GsaI	CCCAGC	1 cut(s)	1262
GsuI	CTGGAG	4 cut(s)	243, 349, 426, 620
HaeII	RGCGCY	2 cut(s)	912, 1329
HaeIII	GGCC	6 cut(s)	170, 350, 401, 508, 956, 1127
HapII	CCGG	5 cut(s)	474, 696, 811, 1003, 1323
HgaI	GACGC	1 cut(s)	329
HhaI	GCGC	5 cut(s)	380, 818, 911, 922, 1328
Hin1I	GRCGYC	2 cut(s)	321, 909
Hin6I	GCGC	5 cut(s)	378, 816, 909, 920, 1326
HinP1I	GCGC	5 cut(s)	378, 816, 909, 920, 1326
HincII	GTYRAC	1 cut(s)	721
HindII	GTYRAC	1 cut(s)	721
HinfI	GANTC	5 cut(s)	496, 713, 1011, 1163, 1232
HpaII	CCGG	5 cut(s)	474, 696, 811, 1003, 1323
HphI	GGTGA	7 cut(s)	445, 550, 577, 652, 850, 1036, 1150
Hpy166II	GTNNAC	3 cut(s)	721, 1027, 1120
Hpy188I	TCNGA	4 cut(s)	501, 1064, 1168, 1195
Hpy188III	TCNNGA	5 cut(s)	194, 787, 929, 1236, 1242
Hpy8I	GTNNAC	3 cut(s)	721, 1027, 1120
Hpy99I	CGWCG	1 cut(s)	722
HpyAV	CCTTC	4 cut(s)	255, 677, 967, 1291
HpyCH4III	ACNGT	3 cut(s)	115, 419, 439
HpyCH4IV	ACGT	3 cut(s)	429, 456, 783
HpyCH4V	TGCA	5 cut(s)	98, 300, 550, 943, 985
HpyF3I	CTNAG	4 cut(s)	195, 207, 1194, 1331
HpySE526I	ACGT	3 cut(s)	429, 456, 783
Hsp92I	GRCGYC	2 cut(s)	321, 909
HspAI	GCGC	5 cut(s)	378, 816, 909, 920, 1326
KasI	GGCGCC	1 cut(s)	908
Ksp22I	TGATCA	1 cut(s)	705
Kzo9I	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
LmnI	GCTCC	4 cut(s)	596, 1117, 1150, 1217
Lsp1109I	GCAGC	5 cut(s)	17, 362, 534, 562, 1322
LweI	GCATC	4 cut(s)	118, 823, 981, 1266
MabI	ACCWGGT	1 cut(s)	1020
MaeII	ACGT	3 cut(s)	429, 456, 783
MaeIII	GTNAC	9 cut(s)	202, 275, 335, 433, 565, 572, 746, 1097, 1138
MalI	GATC	5 cut(s)	707, 791, 1049, 1192, 1240
MboI	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
MboII	GAAGA	7 cut(s)	116, 376, 514, 626, 658, 1006, 1186
MfeI	CAATTG	1 cut(s)	315
MflI	RGATCY	1 cut(s)	1190
MhlI	GDGCHC	2 cut(s)	151, 828
MluCI	AATT	4 cut(s)	315, 343, 945, 1316
Mly113I	GGCGCC	1 cut(s)	909
MlyI	GAGTC	2 cut(s)	707, 1172
MmeI	TCCRAC	5 cut(s)	110, 804, 891, 1248, 1309
Mph1103I	ATGCAT	1 cut(s)	302
MseI	TTAA	2 cut(s)	15, 161
MslI	CAYNNNNRTG	4 cut(s)	428, 677, 803, 891
MspI	CCGG	5 cut(s)	474, 696, 811, 1003, 1323
MspR9I	CCNGG	6 cut(s)	474, 696, 811, 1022, 1094, 1290
MunI	CAATTG	1 cut(s)	315
MvaI	CCWGG	3 cut(s)	1022, 1094, 1290
MvnI	CGCG	1 cut(s)	920
NarI	GGCGCC	1 cut(s)	909
NciI	CCSGG	3 cut(s)	474, 696, 811
NcoI	CCATGG	1 cut(s)	171
NdeI	CATATG	1 cut(s)	726
NdeII	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
NlaIV	GGNNCC	7 cut(s)	421, 478, 598, 775, 910, 1001, 1294
NmeAIII	GCCGAG	2 cut(s)	256, 1020
NmuCI	GTSAC	7 cut(s)	202, 335, 433, 565, 572, 746, 1138
NsbI	TGCGCA	1 cut(s)	817
NsiI	ATGCAT	1 cut(s)	302
NspI	RCATGY	1 cut(s)	802
PciI	ACATGT	1 cut(s)	798
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PfeI	GAWTC	3 cut(s)	496, 1011, 1232
PflFI	GACNNNGTC	1 cut(s)	716
PflMI	CCANNNNNTGG	1 cut(s)	786
PkrI	GCNGC	5 cut(s)	7, 377, 549, 552, 1337
PleI	GAGTC	2 cut(s)	707, 1171
PluTI	GGCGCC	1 cut(s)	912
PpsI	GAGTC	2 cut(s)	707, 1171
Ppu21I	YACGTR	1 cut(s)	430
PpuMI	RGGWCCY	1 cut(s)	1293
PscI	ACATGT	1 cut(s)	798
PsiI	TTATAA	1 cut(s)	288
Psp5II	RGGWCCY	1 cut(s)	1293
Psp6I	CCWGG	3 cut(s)	1020, 1092, 1288
PspEI	GGTNACC	2 cut(s)	565, 1138
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	3 cut(s)	1020, 1092, 1288
PspN4I	GGNNCC	7 cut(s)	421, 478, 598, 775, 910, 1001, 1294
PspPI	GGNCC	8 cut(s)	83, 293, 348, 419, 506, 738, 1000, 1293
PspPPI	RGGWCCY	1 cut(s)	1293
PsrI	GAACNNNNNNTAC	2 cut(s)	1019, 1051
PstI	CTGCAG	1 cut(s)	552
PsuI	RGATCY	1 cut(s)	1190
PsyI	GACNNNGTC	1 cut(s)	716
RsaI	GTAC	3 cut(s)	112, 134, 459
RsaNI	GTAC	3 cut(s)	111, 133, 458
RseI	CAYNNNNRTG	4 cut(s)	428, 677, 803, 891
SalI	GTCGAC	1 cut(s)	719
SaqAI	TTAA	2 cut(s)	15, 161
SatI	GCNGC	5 cut(s)	6, 376, 548, 551, 1336
Sau3AI	GATC	5 cut(s)	705, 789, 1047, 1190, 1238
Sau96I	GGNCC	8 cut(s)	83, 293, 348, 419, 506, 738, 1000, 1293
SchI	GAGTC	2 cut(s)	707, 1172
ScrFI	CCNGG	6 cut(s)	474, 696, 811, 1022, 1094, 1290
SduI	GDGCHC	2 cut(s)	151, 828
SexAI	ACCWGGT	1 cut(s)	1020
SfaNI	GCATC	4 cut(s)	118, 823, 981, 1266
SfcI	CTRYAG	1 cut(s)	548
SfoI	GGCGCC	1 cut(s)	910
SinI	GGWCC	6 cut(s)	83, 293, 419, 738, 1000, 1293
SmiMI	CAYNNNNRTG	4 cut(s)	428, 677, 803, 891
SmlI	CTYRAG	2 cut(s)	268, 927
SmoI	CTYRAG	2 cut(s)	268, 927
Sse9I	AATT	4 cut(s)	315, 343, 945, 1316
SsiI	CCGC	1 cut(s)	852
SspDI	GGCGCC	1 cut(s)	908
StyD4I	CCNGG	6 cut(s)	472, 694, 809, 1020, 1092, 1288
StyI	CCWWGG	3 cut(s)	171, 243, 382
TaaI	ACNGT	3 cut(s)	115, 419, 439
TaiI	ACGT	3 cut(s)	432, 459, 786
TaqI	TCGA	4 cut(s)	71, 720, 792, 831
TasI	AATT	4 cut(s)	315, 343, 945, 1316
TfiI	GAWTC	3 cut(s)	496, 1011, 1232
Tru1I	TTAA	2 cut(s)	15, 161
Tru9I	TTAA	2 cut(s)	15, 161
TscAI	CASTG	5 cut(s)	211, 409, 577, 886, 1049
TseFI	GTSAC	7 cut(s)	202, 335, 433, 565, 572, 746, 1138
TseI	GCWGC	5 cut(s)	5, 375, 547, 550, 1335
Tsp45I	GTSAC	7 cut(s)	202, 335, 433, 565, 572, 746, 1138
TspDTI	ATGAA	4 cut(s)	117, 1088, 1096, 1226
TspGWI	ACGGA	2 cut(s)	706, 1023
TspRI	CASTG	5 cut(s)	211, 409, 577, 886, 1049
Tth111I	GACNNNGTC	1 cut(s)	716
Van91I	CCANNNNNTGG	1 cut(s)	786
VpaK11BI	GGWCC	6 cut(s)	83, 293, 419, 738, 1000, 1293
XagI	CCTNNNNNAGG	1 cut(s)	328
XapI	RAATTY	1 cut(s)	1316
XceI	RCATGY	1 cut(s)	802
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1167
XmiI	GTMKAC	1 cut(s)	720
Zsp2I	ATGCAT	1 cut(s)	302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.