Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0011725

Physical Location & Seq

Genomic Coordinates Reverse (-)

59539527 .. 59541284

Gene Structure 1758 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G032390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGCACTGAATAGACTCGGTTCAATGAATAGACTCGGTTCAATCTTTCTCGTCTTCTTTTTGTTGTCTTGTGTTGCTGTAATTGTCAACGCCGAAAATGATACTGCGGAAGATGAGTACTGGAAAGAACGAGCCGCTGTTGCTATGAAAATTGCCGAGGAATCTGTTCATCCAAATCCCCAGGAAGTTACCGAGGAGATCAATGCTGACGTACACGAGCTTATCATGGGGCAAAATAGCACGAAAAGGAAATTGAGAGGTTCCAAGGGATATGACGGTCCCTGCGAGGCCACCAACCCAATCGATGCATGCTGGCGGTGTGACCCTGATTGGGCCGACAATCGGGAGAAGATCGTCGATTGCGTCAAGGGTTTCGGCAAAAAGACTACCGGCGGCAAGGGTGGGCCCATCTACATTGTCACTGATGACTCAGATGACGACATTGTGAACCCTAAACCAGGAACCCTCCGCGACGCAGTGATCCAGAAAGGTCCTCTATGGATCATCTTTGAGCATAGCATGGTGATTCGTCTGCAACAAGAGCTGATGGTAACAAGTGACAAAACCATCGATGGTAGAGGAGCCAATGTTGTGATTGCCTATGGTGGTGGTATCACTCTTCAGTATGTGAAAAATATCATCATCACGAACATTCACGTTAAGGACATTGAGTCCAAAGCCGGTGGTATGATCAGAGACTGCGTGAATCATTTTGGTTTTAGGACCAGGAGCGATGGCGATGGCATCTCCCTCTTCGGCGCTTCCAACGTCTGGATCGACCATGTTTCCATGGCGAGGTGCGACGACGGACTCATTGACGCTATCATGAGTTCCACTGCTATCACCATCTCCAATTCCCACTTCACTGACCACAACGAGGCAATGTTGTTCGGGGCAAGTAATAGCTACTCTCAAGACAAGATCATGCAAGTAACGGTCGCGTTCAACCACTTTGGGCAGGGGTTGATTCAGAGAATGCCAAGGTGCCGATTTGGATTCTTCCACGTGGTGAACAATGACTACACCCATTGGATAATGTATGCTATTGGTGGTAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTACATTGCTCCTGAAAGCCATGTCGCAAAAGAGGTGACCAAGAGGGAGTACACAGATGAGTCGGAATGGAAGCACTGGAATTGGAGATCCGAGGGAGACTTGATGGTGAATGGAGCATATTTCGTTGAATCCGGGGACCCCAACGTTGCGAAATCAGAGAAGATAGAAATGTTGCCGTTCAAACCCGGGACATTGGCCTCAACCCTTACTAGATTTTCCGGCGCTCTAGAGTGCTGTGTTGGCCAACCATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

447

Amino Acids

49.64

Weight (kDa)

5.49

Isoelectric Point (pI)

28.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	38 - 85	3.3e-13	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	180 - 382	3.5e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14420 AT2G02720
fragaria_vesca	FvH4_4g31900
malus_domestica	MD13G1078400.v1.1 MD16G1078000.v1.1 MD16G1078100.v1.1
prunus_persica	Prupe.1G268500_v2.0.a1 Prupe.1G268700_v2.0.a1
pyrus_communis	pycom13g06810 pycom16g06740 pycom16g06760
rosa_chinensis	RchiOBHm_Chr4g0440551
rosa_rugosa	Rorug04G0326600
rosa_samantha	Rh4AG378300 Rh4BG391000 Rh4CG405700 Rh4DG384700
rosa_wichuraiana	Rw4G032390 Rw4G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	984
AccB7I	CCANNNNNTGG	1 cut(s)	771
AccII	CGCG	2 cut(s)	471, 941
AciI	CCGC	5 cut(s)	107, 135, 316, 393, 469
AclI	AACGTT	1 cut(s)	1233
AclWI	GGATC	4 cut(s)	475, 509, 782, 1170
AcoI	YGGCCR	1 cut(s)	1330
AcuI	CTGAAG	1 cut(s)	605
AcvI	CACGTG	1 cut(s)	1006
AdeI	CACNNNGTG	1 cut(s)	1009
AfaI	GTAC	4 cut(s)	119, 213, 1091, 1139
AfiI	CCNNNNNNNGG	6 cut(s)	331, 342, 458, 771, 795, 877
AgsI	TTSAA	5 cut(s)	24, 42, 946, 1217, 1270
AhdI	GACNNNNNGTC	1 cut(s)	670
AjnI	CCWGG	4 cut(s)	180, 457, 725, 1077
AjuI	GAANNNNNNNTTGG	2 cut(s)	845, 877
AluBI	AGCT	3 cut(s)	220, 544, 906
AluI	AGCT	3 cut(s)	220, 544, 906
Alw26I	GTCTC	2 cut(s)	690, 1179
AlwI	GGATC	4 cut(s)	475, 509, 782, 1170
AlwNI	CAGNNNCTG	1 cut(s)	699
Ama87I	CYCGRG	1 cut(s)	1274
AoxI	GGCC	5 cut(s)	288, 333, 404, 1284, 1330
ApaI	GGGCCC	1 cut(s)	408
Asp700I	GAANNNNTTC	1 cut(s)	165
AspLEI	GCGC	2 cut(s)	761, 1313
AspS9I	GGNCC	7 cut(s)	278, 333, 404, 405, 491, 723, 1225
AsuC2I	CCSGG	3 cut(s)	1222, 1275, 1276
AsuHPI	GGTGA	5 cut(s)	535, 835, 1021, 1135, 1207
AvaI	CYCGRG	1 cut(s)	1274
AvaII	GGWCC	4 cut(s)	278, 491, 723, 1225
BaeGI	GKGCMC	1 cut(s)	408
BalI	TGGCCA	1 cut(s)	1332
BanI	GGYRCC	1 cut(s)	984
BanII	GRGCYC	1 cut(s)	408
BauI	CACGAG	1 cut(s)	215
BbrPI	CACGTG	1 cut(s)	1006
BbsI	GAAGAC	1 cut(s)	46
BccI	CCATC	9 cut(s)	416, 541, 566, 575, 728, 734, 854, 1076, 1186
BceAI	ACGGC	1 cut(s)	1249
BciT130I	CCWGG	4 cut(s)	182, 459, 727, 1079
BclI	TGATCA	1 cut(s)	690
BcnI	CCSGG	3 cut(s)	1222, 1275, 1276
BcoDI	GTCTC	2 cut(s)	690, 1179
BfaI	CTAG	3 cut(s)	1299, 1316, 1342
BfoI	RGCGCY	2 cut(s)	762, 1314
BisI	GCNGC	2 cut(s)	135, 394
BlsI	GCNGC	2 cut(s)	136, 395
BmcAI	AGTACT	1 cut(s)	119
Bme1390I	CCNGG	7 cut(s)	182, 459, 727, 1079, 1222, 1275, 1276
Bme18I	GGWCC	4 cut(s)	278, 491, 723, 1225
BmeRI	GACNNNNNGTC	1 cut(s)	670
BmeT110I	CYCGRG	1 cut(s)	1274
BmgT120I	GGNCC	7 cut(s)	278, 333, 404, 405, 491, 723, 1225
BmiI	GGNNCC	8 cut(s)	262, 280, 406, 463, 583, 986, 1226, 1227
BmrFI	CCNGG	7 cut(s)	182, 459, 727, 1079, 1222, 1275, 1276
BmsI	GCATC	2 cut(s)	295, 753
BpiI	GAAGAC	1 cut(s)	46
BpuEI	CTTGAG	1 cut(s)	897
BpuMI	CCSGG	3 cut(s)	1222, 1275, 1276
Bsa29I	ATCGAT	2 cut(s)	303, 570
BsaAI	YACGTR	1 cut(s)	1006
BsaXI	ACNNNNNCTCC	2 cut(s)	573, 603
Bsc4I	CCNNNNNNNGG	6 cut(s)	331, 342, 458, 771, 795, 877
Bse118I	RCCGGY	2 cut(s)	389, 680
Bse1I	ACTGG	2 cut(s)	125, 1169
Bse3DI	GCAATG	2 cut(s)	888, 1092
BseBI	CCWGG	4 cut(s)	182, 459, 727, 1079
BseCI	ATCGAT	2 cut(s)	303, 570
BseGI	GGATG	1 cut(s)	169
BseLI	CCNNNNNNNGG	6 cut(s)	331, 342, 458, 771, 795, 877
BseMI	GCAATG	2 cut(s)	888, 1092
BseMII	CTCAG	1 cut(s)	444
BseNI	ACTGG	2 cut(s)	125, 1169
BseRI	GAGGAG	2 cut(s)	209, 594
BseSI	GKGCMC	1 cut(s)	408
Bsh1236I	CGCG	2 cut(s)	471, 941
Bsh1285I	CGRYCG	1 cut(s)	939
BshFI	GGCC	5 cut(s)	290, 335, 406, 1286, 1332
BshNI	GGYRCC	1 cut(s)	984
BshVI	ATCGAT	2 cut(s)	303, 570
BsiEI	CGRYCG	1 cut(s)	939
BsiHKCI	CYCGRG	1 cut(s)	1274
BsiSI	CCGG	5 cut(s)	390, 681, 1221, 1275, 1308
BslFI	GGGAC	3 cut(s)	264, 1238, 1291
BslI	CCNNNNNNNGG	6 cut(s)	331, 342, 458, 771, 795, 877
BsmAI	GTCTC	2 cut(s)	690, 1179
BsmFI	GGGAC	3 cut(s)	264, 1238, 1291
BsmI	GAATGC	1 cut(s)	981
BsnI	GGCC	5 cut(s)	290, 335, 406, 1286, 1332
BsoBI	CYCGRG	1 cut(s)	1274
Bsp120I	GGGCCC	1 cut(s)	404
Bsp1286I	GDGCHC	1 cut(s)	408
Bsp143I	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
Bsp19I	CCATGG	1 cut(s)	789
BspACI	CCGC	5 cut(s)	107, 135, 316, 393, 469
BspANI	GGCC	5 cut(s)	290, 335, 406, 1286, 1332
BspCNI	CTCAG	1 cut(s)	443
BspDI	ATCGAT	2 cut(s)	303, 570
BspFNI	CGCG	2 cut(s)	471, 941
BspHI	TCATGA	1 cut(s)	825
BspLI	GGNNCC	8 cut(s)	262, 280, 406, 463, 583, 986, 1226, 1227
BspPI	GGATC	4 cut(s)	475, 509, 782, 1170
BspT107I	GGYRCC	1 cut(s)	984
BsrDI	GCAATG	2 cut(s)	888, 1092
BsrFI	RCCGGY	2 cut(s)	389, 680
BsrI	ACTGG	2 cut(s)	125, 1169
BssAI	RCCGGY	2 cut(s)	389, 680
BssMI	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
BssSI	CACGAG	1 cut(s)	215
BssT1I	CCWWGG	3 cut(s)	264, 789, 980
Bst2BI	CACGAG	1 cut(s)	215
Bst2UI	CCWGG	4 cut(s)	182, 459, 727, 1079
Bst4CI	ACNGT	2 cut(s)	278, 937
Bst6I	CTCTTC	2 cut(s)	624, 758
BstBAI	YACGTR	1 cut(s)	1006
BstC8I	GCNNGC	2 cut(s)	310, 314
BstDEI	CTNAG	1 cut(s)	430
BstDSI	CCRYGG	1 cut(s)	789
BstEII	GGTNACC	1 cut(s)	1123
BstENI	CCTNNNNNAGG	1 cut(s)	456
BstF5I	GGATG	1 cut(s)	169
BstFNI	CGCG	2 cut(s)	471, 941
BstH2I	RGCGCY	2 cut(s)	762, 1314
BstHHI	GCGC	2 cut(s)	761, 1313
BstKTI	GATC	8 cut(s)	201, 354, 483, 504, 693, 777, 924, 1178
BstMAI	GTCTC	2 cut(s)	690, 1179
BstMBI	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
BstMCI	CGRYCG	1 cut(s)	939
BstMWI	GCNNNNNNNGC	4 cut(s)	140, 541, 1329, 1338
BstNI	CCWGG	4 cut(s)	182, 459, 727, 1079
BstNSI	RCATGY	1 cut(s)	312
BstPI	GGTNACC	1 cut(s)	1123
BstSCI	CCNGG	7 cut(s)	180, 457, 725, 1077, 1220, 1273, 1274
BstSLI	GKGCMC	1 cut(s)	408
BstUI	CGCG	2 cut(s)	471, 941
BstV2I	GAAGAC	1 cut(s)	46
BstX2I	RGATCY	1 cut(s)	1175
BstYI	RGATCY	1 cut(s)	1175
Bsu15I	ATCGAT	2 cut(s)	303, 570
BsuRI	GGCC	5 cut(s)	290, 335, 406, 1286, 1332
BsuTUI	ATCGAT	2 cut(s)	303, 570
BtgI	CCRYGG	1 cut(s)	789
BtgZI	GCGATG	2 cut(s)	747, 753
BtsCI	GGATG	1 cut(s)	169
BtsI	GCAGTG	2 cut(s)	483, 834
BtsIMutI	CAGTG	6 cut(s)	5, 420, 483, 834, 864, 1162
Cac8I	GCNNGC	2 cut(s)	310, 314
CaiI	CAGNNNCTG	1 cut(s)	699
CciI	TCATGA	1 cut(s)	825
CfoI	GCGC	2 cut(s)	761, 1313
Cfr10I	RCCGGY	2 cut(s)	389, 680
Cfr13I	GGNCC	7 cut(s)	278, 333, 404, 405, 491, 723, 1225
Cfr9I	CCCGGG	1 cut(s)	1274
ClaI	ATCGAT	2 cut(s)	303, 570
CseI	GACGC	3 cut(s)	352, 482, 827
Csp6I	GTAC	4 cut(s)	118, 212, 1090, 1138
CspCI	CAANNNNNGTGG	2 cut(s)	664, 699
CviQI	GTAC	4 cut(s)	118, 212, 1090, 1138
DdeI	CTNAG	1 cut(s)	430
DpnI	GATC	8 cut(s)	200, 353, 482, 503, 692, 776, 923, 1177
DpnII	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
DraIII	CACNNNGTG	1 cut(s)	1009
DriI	GACNNNNNGTC	1 cut(s)	670
EaeI	YGGCCR	1 cut(s)	1330
Eam1104I	CTCTTC	2 cut(s)	624, 758
Eam1105I	GACNNNNNGTC	1 cut(s)	670
EarI	CTCTTC	2 cut(s)	624, 758
Eco130I	CCWWGG	3 cut(s)	264, 789, 980
Eco24I	GRGCYC	1 cut(s)	408
Eco47I	GGWCC	4 cut(s)	278, 491, 723, 1225
Eco57I	CTGAAG	1 cut(s)	605
Eco72I	CACGTG	1 cut(s)	1006
Eco88I	CYCGRG	1 cut(s)	1274
Eco91I	GGTNACC	1 cut(s)	1123
EcoNI	CCTNNNNNAGG	1 cut(s)	456
EcoO109I	RGGNCCY	2 cut(s)	491, 1225
EcoO65I	GGTNACC	1 cut(s)	1123
EcoRII	CCWGG	4 cut(s)	180, 457, 725, 1077
EcoT14I	CCWWGG	3 cut(s)	264, 789, 980
EcoT22I	ATGCAT	1 cut(s)	310
EcoT38I	GRGCYC	1 cut(s)	408
ErhI	CCWWGG	3 cut(s)	264, 789, 980
FaqI	GGGAC	3 cut(s)	264, 1238, 1291
FbaI	TGATCA	1 cut(s)	690
Fnu4HI	GCNGC	2 cut(s)	135, 394
FokI	GGATG	1 cut(s)	156
FriOI	GRGCYC	1 cut(s)	408
Fsp4HI	GCNGC	2 cut(s)	135, 394
FspBI	CTAG	3 cut(s)	1299, 1316, 1342
GlaI	GCGC	2 cut(s)	760, 1312
GluI	GCNGC	2 cut(s)	135, 394
HaeII	RGCGCY	2 cut(s)	762, 1314
HaeIII	GGCC	5 cut(s)	290, 335, 406, 1286, 1332
HapII	CCGG	5 cut(s)	390, 681, 1221, 1275, 1308
HgaI	GACGC	3 cut(s)	352, 482, 827
HhaI	GCGC	2 cut(s)	761, 1313
Hin6I	GCGC	2 cut(s)	759, 1311
HinP1I	GCGC	2 cut(s)	759, 1311
HincII	GTYRAC	1 cut(s)	88
HindII	GTYRAC	1 cut(s)	88
HpaII	CCGG	5 cut(s)	390, 681, 1221, 1275, 1308
HphI	GGTGA	5 cut(s)	535, 835, 1021, 1135, 1207
Hpy166II	GTNNAC	5 cut(s)	88, 214, 448, 1012, 1140
Hpy188I	TCNGA	6 cut(s)	433, 695, 972, 1153, 1180, 1246
Hpy188III	TCNNGA	8 cut(s)	344, 484, 646, 772, 826, 914, 1100, 1316
Hpy8I	GTNNAC	5 cut(s)	88, 214, 448, 1012, 1140
Hpy99I	CGWCG	4 cut(s)	359, 476, 806, 809
HpyCH4III	ACNGT	2 cut(s)	278, 937
HpyCH4IV	ACGT	5 cut(s)	210, 657, 768, 1005, 1233
HpyCH4V	TGCA	3 cut(s)	308, 535, 928
HpyF10VI	GCNNNNNNNGC	4 cut(s)	140, 541, 1329, 1338
HpyF3I	CTNAG	1 cut(s)	430
HpySE526I	ACGT	5 cut(s)	210, 657, 768, 1005, 1233
HspAI	GCGC	2 cut(s)	759, 1311
KflI	GGGWCCC	1 cut(s)	1225
Ksp22I	TGATCA	1 cut(s)	690
Kzo9I	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
LmnI	GCTCC	4 cut(s)	581, 729, 1102, 1202
LweI	GCATC	2 cut(s)	295, 753
MaeI	CTAG	3 cut(s)	1299, 1316, 1342
MaeII	ACGT	5 cut(s)	210, 657, 768, 1005, 1233
MaeIII	GTNAC	9 cut(s)	187, 320, 418, 550, 557, 931, 1052, 1082, 1123
MalI	GATC	8 cut(s)	200, 353, 482, 503, 692, 776, 923, 1177
MboI	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
MboII	GAAGA	7 cut(s)	46, 122, 361, 611, 745, 991, 1261
MflI	RGATCY	1 cut(s)	1175
MhlI	GDGCHC	1 cut(s)	408
MlsI	TGGCCA	1 cut(s)	1332
MluCI	AATT	5 cut(s)	81, 150, 251, 853, 1168
MluNI	TGGCCA	1 cut(s)	1332
MlyI	GAGTC	6 cut(s)	9, 27, 422, 680, 804, 1157
MmeI	TCCRAC	2 cut(s)	789, 1131
Mox20I	TGGCCA	1 cut(s)	1332
Mph1103I	ATGCAT	1 cut(s)	310
MroXI	GAANNNNTTC	1 cut(s)	165
MscI	TGGCCA	1 cut(s)	1332
MseI	TTAA	1 cut(s)	660
Msp20I	TGGCCA	1 cut(s)	1332
MspA1I	CMGCKG	1 cut(s)	137
MspI	CCGG	5 cut(s)	390, 681, 1221, 1275, 1308
MspR9I	CCNGG	7 cut(s)	182, 459, 727, 1079, 1222, 1275, 1276
Mva1269I	GAATGC	1 cut(s)	981
MvaI	CCWGG	4 cut(s)	182, 459, 727, 1079
MvnI	CGCG	2 cut(s)	471, 941
MwoI	GCNNNNNNNGC	4 cut(s)	140, 541, 1329, 1338
NciI	CCSGG	3 cut(s)	1222, 1275, 1276
NcoI	CCATGG	1 cut(s)	789
NdeII	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
NlaIV	GGNNCC	8 cut(s)	262, 280, 406, 463, 583, 986, 1226, 1227
NmeAIII	GCCGAG	1 cut(s)	181
NmuCI	GTSAC	4 cut(s)	320, 418, 557, 1123
NsiI	ATGCAT	1 cut(s)	310
NspI	RCATGY	1 cut(s)	312
PaeI	GCATGC	1 cut(s)	312
PagI	TCATGA	1 cut(s)	825
PcsI	WCGNNNNNNNCGW	3 cut(s)	282, 360, 774
PctI	GAATGC	1 cut(s)	981
PdmI	GAANNNNTTC	1 cut(s)	165
PfeI	GAWTC	6 cut(s)	161, 526, 706, 967, 996, 1217
PflMI	CCANNNNNTGG	1 cut(s)	771
PkrI	GCNGC	2 cut(s)	136, 395
PleI	GAGTC	6 cut(s)	9, 27, 422, 679, 804, 1156
PmaCI	CACGTG	1 cut(s)	1006
PmlI	CACGTG	1 cut(s)	1006
PpsI	GAGTC	6 cut(s)	9, 27, 422, 679, 804, 1156
Ppu21I	YACGTR	1 cut(s)	1006
PpuMI	RGGWCCY	2 cut(s)	491, 1225
Psp1406I	AACGTT	1 cut(s)	1233
Psp5II	RGGWCCY	2 cut(s)	491, 1225
Psp6I	CCWGG	4 cut(s)	180, 457, 725, 1077
PspCI	CACGTG	1 cut(s)	1006
PspEI	GGTNACC	1 cut(s)	1123
PspGI	CCWGG	4 cut(s)	180, 457, 725, 1077
PspN4I	GGNNCC	8 cut(s)	262, 280, 406, 463, 583, 986, 1226, 1227
PspOMI	GGGCCC	1 cut(s)	404
PspPI	GGNCC	7 cut(s)	278, 333, 404, 405, 491, 723, 1225
PspPPI	RGGWCCY	2 cut(s)	491, 1225
PsrI	GAACNNNNNNTAC	2 cut(s)	1004, 1036
PstNI	CAGNNNCTG	1 cut(s)	699
PsuI	RGATCY	1 cut(s)	1175
RsaI	GTAC	4 cut(s)	119, 213, 1091, 1139
RsaNI	GTAC	4 cut(s)	118, 212, 1090, 1138
SaqAI	TTAA	1 cut(s)	660
SatI	GCNGC	2 cut(s)	135, 394
Sau3AI	GATC	8 cut(s)	198, 351, 480, 501, 690, 774, 921, 1175
Sau96I	GGNCC	7 cut(s)	278, 333, 404, 405, 491, 723, 1225
ScaI	AGTACT	1 cut(s)	119
SchI	GAGTC	6 cut(s)	9, 27, 422, 680, 804, 1157
ScrFI	CCNGG	7 cut(s)	182, 459, 727, 1079, 1222, 1275, 1276
SduI	GDGCHC	1 cut(s)	408
SfaNI	GCATC	2 cut(s)	295, 753
SinI	GGWCC	4 cut(s)	278, 491, 723, 1225
SmaI	CCCGGG	1 cut(s)	1276
SmlI	CTYRAG	1 cut(s)	912
SmoI	CTYRAG	1 cut(s)	912
SphI	GCATGC	1 cut(s)	312
Sse9I	AATT	5 cut(s)	81, 150, 251, 853, 1168
SsiI	CCGC	5 cut(s)	107, 135, 316, 393, 469
SspMI	CTAG	3 cut(s)	1299, 1316, 1342
StyD4I	CCNGG	7 cut(s)	180, 457, 725, 1077, 1220, 1273, 1274
StyI	CCWWGG	3 cut(s)	264, 789, 980
TaaI	ACNGT	2 cut(s)	278, 937
TaiI	ACGT	5 cut(s)	213, 660, 771, 1008, 1236
TaqI	TCGA	4 cut(s)	303, 357, 570, 777
TasI	AATT	5 cut(s)	81, 150, 251, 853, 1168
TatI	WGTACW	2 cut(s)	117, 1137
TauI	GCSGC	2 cut(s)	137, 396
TfiI	GAWTC	6 cut(s)	161, 526, 706, 967, 996, 1217
Tru1I	TTAA	1 cut(s)	660
Tru9I	TTAA	1 cut(s)	660
TscAI	CASTG	6 cut(s)	12, 427, 483, 841, 871, 1169
TseFI	GTSAC	4 cut(s)	320, 418, 557, 1123
Tsp45I	GTSAC	4 cut(s)	320, 418, 557, 1123
TspDTI	ATGAA	4 cut(s)	41, 158, 161, 1073
TspGWI	ACGGA	1 cut(s)	822
TspMI	CCCGGG	1 cut(s)	1274
TspRI	CASTG	6 cut(s)	12, 427, 483, 841, 871, 1169
Van91I	CCANNNNNTGG	1 cut(s)	771
VpaK11BI	GGWCC	4 cut(s)	278, 491, 723, 1225
XagI	CCTNNNNNAGG	1 cut(s)	456
XbaI	TCTAGA	1 cut(s)	1315
XceI	RCATGY	1 cut(s)	312
XmaI	CCCGGG	1 cut(s)	1274
XmnI	GAANNNNTTC	1 cut(s)	165
XspI	CTAG	3 cut(s)	1299, 1316, 1342
ZrmI	AGTACT	1 cut(s)	119
Zsp2I	ATGCAT	1 cut(s)	310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.