Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011725

Physical Location & Seq

Genomic Coordinates Reverse (-)

5481558 .. 5483765

Gene Structure 2208 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1078100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGTAGCCATGTTTAACTCGTCCTATTTCTTTCTATTTTTGTTTTTCTTGTACTTTGCTGTATTCGTTTCGAGCAACGATAATCCAACCGATGTTCCAAATGAAGATACGCTGGTATTAGATTTAGATGCGTACTGGAAAGAACGAGCCGAAGAAGCTGAAAAGGTGGCCATGGAATCCTACAATAAAAATCCTGAGAAAGTCACTGAAGAGTTCAATACTGAAGTTGGCAAGCTTATGTCGAAGGAAAACACCACAAGAAGGAACTTGAGGGGTAACAAGGCTTATACGGGTCCATGCATGGCGACCAACCCAATGGACGCCTGTTGGAGGTGTGACCCTAACTGGGCCAACAACCGAAAGAAGATTGTAGGCTGCGCCCAAGGCTTCGGTAAAAAGACCACCGGAGGGAAGGATGGTCCTTTTTACGTGGTGACAATGGGCACAGATGATGACGTGCAAAACCCTAAACCAGGAACCCTACGCCATGCCGTGATTCAGAAAGGGCCTTTGTGGATCATCTTCACAAAAAGCATGGTGATTAGGCTGCAGCAAGAGCTGATGGTGACTAGTGACAAGACCATTGATGCTAGGGGAGCCAATGTTGTGATAGAAGAGGGTGCTGGCATTACCCTGCAGTTTGTGAAGAATGTAATCATCACCAACCTTCACATAAAAATGATTGTCACCAAACCCGGTGGCCTGATCAGGGACTCTGTCGACCACATAGGTTTAAGGACCCAGAGTGACGGCGATGGTATCTCCCTCTTTGGTGCCTCCAACGTCTGGATCGATCACGTTTCCATGTCCAGGTGCGCAGATGGGCTCATCGACGCCATCATGGGTTCCACCGCCATCACCATCTCCAACTCCCACTTCACTGACCACGATGAGGCGATGTTGTTTGGTGCCAACAACGCCCACACTCAAGACAAAATCATGCAAATTACTTTGGCCTTCAACCACTTTGGTCAGGGACTGGTGCAGAGGATGCCAAGGGTCCGGCACGGATTCTTCCACGTGGTGAACAACGACTACACCCACTGGATCATGTATGCCATCGGAGGAAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTTCATTGCTCCCATTAACGGGGTCACCAAACAGGTGACCCATAGGGAGAATACACCAGAGGCAGAGTGGAAGAACTGGGAATGGAGATCTGAGGGAGATTTGATGATGAATGGAGCTTTCTTTGTTGAATCTGGATCAGGAGCCAGCAAACACCCAGCAAAGTTGGACCTGATGCCCTTCAAACCAGGGACCCTTGTCTCAACGCTCACAAAATTTTCCGGTGCTCTAAACTGTGCTGTCGGCAAACCCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

453

Amino Acids

50.25

Weight (kDa)

6.67

Isoelectric Point (pI)

25.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	41 - 90	7.2e-19	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	186 - 379	4.8e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14420 AT2G02720
fragaria_vesca	FvH4_4g31900
malus_domestica	MD13G1078400.v1.1 MD16G1078000.v1.1 MD16G1078100.v1.1
prunus_persica	Prupe.1G268500_v2.0.a1 Prupe.1G268700_v2.0.a1
pyrus_communis	pycom13g06810 pycom16g06740 pycom16g06760
rosa_chinensis	RchiOBHm_Chr4g0440551
rosa_rugosa	Rorug04G0326600
rosa_samantha	Rh4AG378300 Rh4BG391000 Rh4CG405700 Rh4DG384700
rosa_wichuraiana	Rw4G032390 Rw4G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	817
AccB1I	GGYRCC	2 cut(s)	773, 908
AccB7I	CCANNNNNTGG	1 cut(s)	786
AccI	GTMKAC	1 cut(s)	720
AciI	CCGC	1 cut(s)	852
AclWI	GGATC	4 cut(s)	524, 797, 1055, 1246
AcoI	YGGCCR	1 cut(s)	168
AcsI	RAATTY	1 cut(s)	1316
AcuI	CTGAAG	2 cut(s)	228, 243
AcvI	CACGTG	1 cut(s)	1021
AcyI	GRCGYC	2 cut(s)	321, 834
AdeI	CACNNNGTG	1 cut(s)	1024
AfaI	GTAC	2 cut(s)	53, 134
AgsI	TTSAA	4 cut(s)	217, 961, 1232, 1285
AhlI	ACTAGT	1 cut(s)	569
AjiI	CACGTC	1 cut(s)	457
AjnI	CCWGG	4 cut(s)	472, 809, 1092, 1288
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AluBI	AGCT	4 cut(s)	158, 235, 559, 1220
AluI	AGCT	4 cut(s)	158, 235, 559, 1220
Alw21I	GWGCWC	1 cut(s)	1330
Alw26I	GTCTC	1 cut(s)	1306
AlwI	GGATC	4 cut(s)	524, 797, 1055, 1246
AlwNI	CAGNNNCTG	1 cut(s)	979
AoxI	GGCC	5 cut(s)	168, 348, 506, 700, 954
ApeKI	GCWGC	3 cut(s)	375, 547, 550
ApoI	RAATTY	1 cut(s)	1316
AspLEI	GCGC	2 cut(s)	380, 818
AspS9I	GGNCC	8 cut(s)	293, 348, 419, 506, 738, 1000, 1270, 1293
AsuC2I	CCSGG	1 cut(s)	696
AsuHPI	GGTGA	9 cut(s)	445, 550, 577, 652, 679, 850, 1036, 1120, 1150
AvaII	GGWCC	6 cut(s)	293, 419, 738, 1000, 1270, 1293
BaeGI	GKGCMC	1 cut(s)	446
BaeI	ACNNNNGTAYC	2 cut(s)	99, 132
BalI	TGGCCA	1 cut(s)	170
BanI	GGYRCC	2 cut(s)	773, 908
BanII	GRGCYC	1 cut(s)	828
BbrPI	CACGTG	1 cut(s)	1021
Bbv12I	GWGCWC	1 cut(s)	1330
BbvI	GCAGC	3 cut(s)	362, 534, 562
BccI	CCATC	9 cut(s)	410, 556, 749, 815, 845, 863, 869, 1067, 1091
BceAI	ACGGC	2 cut(s)	476, 766
BciT130I	CCWGG	4 cut(s)	474, 811, 1094, 1290
BclI	TGATCA	1 cut(s)	705
BcnI	CCSGG	1 cut(s)	696
BcoDI	GTCTC	1 cut(s)	1306
BcuI	ACTAGT	1 cut(s)	569
BfaI	CTAG	3 cut(s)	570, 591, 1357
BfmI	CTRYAG	2 cut(s)	548, 635
BglII	AGATCT	1 cut(s)	1190
BisI	GCNGC	3 cut(s)	376, 548, 551
BlsI	GCNGC	3 cut(s)	377, 549, 552
Bme1390I	CCNGG	5 cut(s)	474, 696, 811, 1094, 1290
Bme18I	GGWCC	6 cut(s)	293, 419, 738, 1000, 1270, 1293
BmgBI	CACGTC	1 cut(s)	457
BmgT120I	GGNCC	8 cut(s)	293, 348, 419, 506, 738, 1000, 1270, 1293
BmrFI	CCNGG	5 cut(s)	474, 696, 811, 1094, 1290
BmrI	ACTGGG	2 cut(s)	355, 1189
BmsI	GCATC	4 cut(s)	118, 577, 981, 1266
BmuI	ACTGGG	2 cut(s)	355, 1189
BoxI	GACNNNNGTC	1 cut(s)	1298
BpuEI	CTTGAG	2 cut(s)	289, 912
BpuMI	CCSGG	1 cut(s)	696
Bsa29I	ATCGAT	1 cut(s)	792
BsaAI	YACGTR	2 cut(s)	430, 1021
BsaHI	GRCGYC	2 cut(s)	321, 834
BsaJI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BsaWI	WCCGGW	2 cut(s)	404, 1322
BsaXI	ACNNNNNCTCC	2 cut(s)	588, 618
Bse1I	ACTGG	5 cut(s)	140, 350, 984, 1049, 1184
Bse3DI	GCAATG	1 cut(s)	1107
BseBI	CCWGG	4 cut(s)	474, 811, 1094, 1290
BseCI	ATCGAT	1 cut(s)	792
BseDI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BseGI	GGATG	2 cut(s)	421, 996
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	2 cut(s)	186, 1185
BseNI	ACTGG	5 cut(s)	140, 350, 984, 1049, 1184
BseSI	GKGCMC	1 cut(s)	446
BseXI	GCAGC	3 cut(s)	362, 534, 562
BseYI	CCCAGC	1 cut(s)	1258
BsgI	GTGCAG	1 cut(s)	1004
BshFI	GGCC	5 cut(s)	170, 350, 508, 702, 956
BshNI	GGYRCC	2 cut(s)	773, 908
BshVI	ATCGAT	1 cut(s)	792
BsiHKAI	GWGCWC	1 cut(s)	1330
BsiSI	CCGG	4 cut(s)	405, 696, 1003, 1323
BslFI	GGGAC	3 cut(s)	725, 990, 1306
BsmAI	GTCTC	1 cut(s)	1306
BsmFI	GGGAC	3 cut(s)	725, 990, 1306
BsnI	GGCC	5 cut(s)	170, 350, 508, 702, 956
Bsp1286I	GDGCHC	3 cut(s)	446, 828, 1330
Bsp143I	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
Bsp19I	CCATGG	1 cut(s)	171
BspACI	CCGC	1 cut(s)	852
BspANI	GGCC	5 cut(s)	170, 350, 508, 702, 956
BspCNI	CTCAG	2 cut(s)	187, 1186
BspDI	ATCGAT	1 cut(s)	792
BspMAI	CTGCAG	2 cut(s)	552, 639
BspPI	GGATC	4 cut(s)	524, 797, 1055, 1246
BspT107I	GGYRCC	2 cut(s)	773, 908
BsrDI	GCAATG	1 cut(s)	1107
BsrI	ACTGG	5 cut(s)	140, 350, 984, 1049, 1184
BssECI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BssMI	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
BssNI	GRCGYC	2 cut(s)	321, 834
BssT1I	CCWWGG	3 cut(s)	171, 382, 995
Bst2UI	CCWGG	4 cut(s)	474, 811, 1094, 1290
Bst4CI	ACNGT	1 cut(s)	1337
Bst6I	CTCTTC	2 cut(s)	204, 609
BstACI	GRCGYC	2 cut(s)	321, 834
BstAPI	GCANNNNNTGC	2 cut(s)	991, 1353
BstBAI	YACGTR	2 cut(s)	430, 1021
BstC8I	GCNNGC	3 cut(s)	233, 625, 1249
BstDEI	CTNAG	2 cut(s)	195, 1194
BstDSI	CCRYGG	1 cut(s)	171
BstEII	GGTNACC	2 cut(s)	1126, 1138
BstENI	CCTNNNNNAGG	2 cut(s)	328, 471
BstF5I	GGATG	2 cut(s)	421, 996
BstHHI	GCGC	2 cut(s)	380, 818
BstKTI	GATC	7 cut(s)	519, 708, 792, 796, 1050, 1193, 1241
BstMAI	GTCTC	1 cut(s)	1306
BstMBI	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
BstMWI	GCNNNNNNNGC	7 cut(s)	155, 384, 556, 596, 917, 991, 1353
BstNI	CCWGG	4 cut(s)	474, 811, 1094, 1290
BstPAI	GACNNNNGTC	1 cut(s)	1298
BstPI	GGTNACC	2 cut(s)	1126, 1138
BstSCI	CCNGG	5 cut(s)	472, 694, 809, 1092, 1288
BstSFI	CTRYAG	2 cut(s)	548, 635
BstSLI	GKGCMC	1 cut(s)	446
BstV1I	GCAGC	3 cut(s)	362, 534, 562
BstX2I	RGATCY	1 cut(s)	1190
BstXI	CCANNNNNNTGG	1 cut(s)	316
BstYI	RGATCY	1 cut(s)	1190
Bsu15I	ATCGAT	1 cut(s)	792
BsuRI	GGCC	5 cut(s)	170, 350, 508, 702, 956
BsuTUI	ATCGAT	1 cut(s)	792
BtgI	CCRYGG	1 cut(s)	171
BtgZI	GCGATG	2 cut(s)	768, 911
BtrI	CACGTC	1 cut(s)	457
BtsCI	GGATG	2 cut(s)	421, 996
BtsIMutI	CAGTG	3 cut(s)	204, 879, 1042
Cac8I	GCNNGC	3 cut(s)	233, 625, 1249
CaiI	CAGNNNCTG	1 cut(s)	979
CfoI	GCGC	2 cut(s)	380, 818
Cfr13I	GGNCC	8 cut(s)	293, 348, 419, 506, 738, 1000, 1270, 1293
ClaI	ATCGAT	1 cut(s)	792
CseI	GACGC	2 cut(s)	329, 842
Csp6I	GTAC	2 cut(s)	52, 133
CspCI	CAANNNNNGTGG	2 cut(s)	679, 714
CviQI	GTAC	2 cut(s)	52, 133
DdeI	CTNAG	2 cut(s)	195, 1194
DpnI	GATC	7 cut(s)	518, 707, 791, 795, 1049, 1192, 1240
DpnII	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
DraIII	CACNNNGTG	1 cut(s)	1024
EaeI	YGGCCR	1 cut(s)	168
Eam1104I	CTCTTC	2 cut(s)	204, 609
EarI	CTCTTC	2 cut(s)	204, 609
Eco130I	CCWWGG	3 cut(s)	171, 382, 995
Eco24I	GRGCYC	1 cut(s)	828
Eco47I	GGWCC	6 cut(s)	293, 419, 738, 1000, 1270, 1293
Eco57I	CTGAAG	2 cut(s)	228, 243
Eco72I	CACGTG	1 cut(s)	1021
Eco91I	GGTNACC	2 cut(s)	1126, 1138
EcoNI	CCTNNNNNAGG	2 cut(s)	328, 471
EcoO109I	RGGNCCY	3 cut(s)	506, 738, 1293
EcoO65I	GGTNACC	2 cut(s)	1126, 1138
EcoRII	CCWGG	4 cut(s)	472, 809, 1092, 1288
EcoT14I	CCWWGG	3 cut(s)	171, 382, 995
EcoT22I	ATGCAT	1 cut(s)	302
EcoT38I	GRGCYC	1 cut(s)	828
ErhI	CCWWGG	3 cut(s)	171, 382, 995
FaqI	GGGAC	3 cut(s)	725, 990, 1306
FbaI	TGATCA	1 cut(s)	705
FblI	GTMKAC	1 cut(s)	720
Fnu4HI	GCNGC	3 cut(s)	376, 548, 551
FokI	GGATG	2 cut(s)	428, 1003
FriOI	GRGCYC	1 cut(s)	828
Fsp4HI	GCNGC	3 cut(s)	376, 548, 551
FspBI	CTAG	3 cut(s)	570, 591, 1357
FspI	TGCGCA	1 cut(s)	817
GlaI	GCGC	2 cut(s)	379, 817
GluI	GCNGC	3 cut(s)	376, 548, 551
GsaI	CCCAGC	1 cut(s)	1262
HaeIII	GGCC	5 cut(s)	170, 350, 508, 702, 956
HapII	CCGG	4 cut(s)	405, 696, 1003, 1323
HgaI	GACGC	2 cut(s)	329, 842
HhaI	GCGC	2 cut(s)	380, 818
Hin1I	GRCGYC	2 cut(s)	321, 834
Hin6I	GCGC	2 cut(s)	378, 816
HinP1I	GCGC	2 cut(s)	378, 816
HincII	GTYRAC	1 cut(s)	721
HindII	GTYRAC	1 cut(s)	721
HindIII	AAGCTT	1 cut(s)	233
HinfI	GANTC	5 cut(s)	176, 496, 713, 1011, 1232
HpaII	CCGG	4 cut(s)	405, 696, 1003, 1323
HphI	GGTGA	9 cut(s)	445, 550, 577, 652, 679, 850, 1036, 1120, 1150
Hpy166II	GTNNAC	2 cut(s)	721, 1027
Hpy188I	TCNGA	3 cut(s)	501, 1064, 1195
Hpy188III	TCNNGA	5 cut(s)	194, 787, 929, 1236, 1242
Hpy8I	GTNNAC	2 cut(s)	721, 1027
Hpy99I	CGWCG	1 cut(s)	836
HpyAV	CCTTC	6 cut(s)	238, 255, 406, 677, 967, 1291
HpyCH4III	ACNGT	1 cut(s)	1337
HpyCH4IV	ACGT	5 cut(s)	429, 456, 783, 798, 1020
HpyCH4V	TGCA	6 cut(s)	300, 460, 550, 637, 943, 985
HpyF10VI	GCNNNNNNNGC	7 cut(s)	155, 384, 556, 596, 917, 991, 1353
HpyF3I	CTNAG	2 cut(s)	195, 1194
HpySE526I	ACGT	5 cut(s)	429, 456, 783, 798, 1020
Hsp92I	GRCGYC	2 cut(s)	321, 834
HspAI	GCGC	2 cut(s)	378, 816
KflI	GGGWCCC	1 cut(s)	1293
Ksp22I	TGATCA	1 cut(s)	705
Kzo9I	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
LmnI	GCTCC	4 cut(s)	596, 1117, 1217, 1244
Lsp1109I	GCAGC	3 cut(s)	362, 534, 562
LweI	GCATC	4 cut(s)	118, 577, 981, 1266
MaeI	CTAG	3 cut(s)	570, 591, 1357
MaeII	ACGT	5 cut(s)	429, 456, 783, 798, 1020
MalI	GATC	7 cut(s)	518, 707, 791, 795, 1049, 1192, 1240
MboI	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
MboII	GAAGA	9 cut(s)	116, 164, 221, 376, 514, 626, 658, 1006, 1186
MflI	RGATCY	1 cut(s)	1190
MhlI	GDGCHC	3 cut(s)	446, 828, 1330
MlsI	TGGCCA	1 cut(s)	170
MluCI	AATT	2 cut(s)	945, 1316
MluNI	TGGCCA	1 cut(s)	170
MlyI	GAGTC	1 cut(s)	707
MmeI	TCCRAC	5 cut(s)	110, 308, 804, 891, 1248
Mox20I	TGGCCA	1 cut(s)	170
Mph1103I	ATGCAT	1 cut(s)	302
MscI	TGGCCA	1 cut(s)	170
MseI	TTAA	3 cut(s)	15, 734, 1119
MslI	CAYNNNNRTG	1 cut(s)	677
Msp20I	TGGCCA	1 cut(s)	170
MspI	CCGG	4 cut(s)	405, 696, 1003, 1323
MspR9I	CCNGG	5 cut(s)	474, 696, 811, 1094, 1290
MvaI	CCWGG	4 cut(s)	474, 811, 1094, 1290
MwoI	GCNNNNNNNGC	7 cut(s)	155, 384, 556, 596, 917, 991, 1353
NciI	CCSGG	1 cut(s)	696
NcoI	CCATGG	1 cut(s)	171
NdeII	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
NmuCI	GTSAC	9 cut(s)	202, 335, 433, 565, 572, 685, 746, 1126, 1138
NsbI	TGCGCA	1 cut(s)	817
NsiI	ATGCAT	1 cut(s)	302
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PfeI	GAWTC	4 cut(s)	176, 496, 1011, 1232
PflFI	GACNNNGTC	1 cut(s)	716
PflMI	CCANNNNNTGG	1 cut(s)	786
PkrI	GCNGC	3 cut(s)	377, 549, 552
PleI	GAGTC	1 cut(s)	707
PmaCI	CACGTG	1 cut(s)	1021
PmlI	CACGTG	1 cut(s)	1021
PpsI	GAGTC	1 cut(s)	707
Ppu21I	YACGTR	2 cut(s)	430, 1021
PpuMI	RGGWCCY	2 cut(s)	738, 1293
PshAI	GACNNNNGTC	1 cut(s)	1298
Psp5II	RGGWCCY	2 cut(s)	738, 1293
Psp6I	CCWGG	4 cut(s)	472, 809, 1092, 1288
PspCI	CACGTG	1 cut(s)	1021
PspEI	GGTNACC	2 cut(s)	1126, 1138
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	4 cut(s)	472, 809, 1092, 1288
PspPI	GGNCC	8 cut(s)	293, 348, 419, 506, 738, 1000, 1270, 1293
PspPPI	RGGWCCY	2 cut(s)	738, 1293
PsrI	GAACNNNNNNTAC	2 cut(s)	1019, 1051
PstI	CTGCAG	2 cut(s)	552, 639
PstNI	CAGNNNCTG	1 cut(s)	979
PsuI	RGATCY	1 cut(s)	1190
PsyI	GACNNNGTC	1 cut(s)	716
RsaI	GTAC	2 cut(s)	53, 134
RsaNI	GTAC	2 cut(s)	52, 133
RseI	CAYNNNNRTG	1 cut(s)	677
SalI	GTCGAC	1 cut(s)	719
SaqAI	TTAA	3 cut(s)	15, 734, 1119
SatI	GCNGC	3 cut(s)	376, 548, 551
Sau3AI	GATC	7 cut(s)	516, 705, 789, 793, 1047, 1190, 1238
Sau96I	GGNCC	8 cut(s)	293, 348, 419, 506, 738, 1000, 1270, 1293
SchI	GAGTC	1 cut(s)	707
ScrFI	CCNGG	5 cut(s)	474, 696, 811, 1094, 1290
SduI	GDGCHC	3 cut(s)	446, 828, 1330
SfaNI	GCATC	4 cut(s)	118, 577, 981, 1266
SfcI	CTRYAG	2 cut(s)	548, 635
SinI	GGWCC	6 cut(s)	293, 419, 738, 1000, 1270, 1293
SmiMI	CAYNNNNRTG	1 cut(s)	677
SmlI	CTYRAG	2 cut(s)	268, 927
SmoI	CTYRAG	2 cut(s)	268, 927
SpeI	ACTAGT	1 cut(s)	569
Sse9I	AATT	2 cut(s)	945, 1316
SsiI	CCGC	1 cut(s)	852
SspMI	CTAG	3 cut(s)	570, 591, 1357
StyD4I	CCNGG	5 cut(s)	472, 694, 809, 1092, 1288
StyI	CCWWGG	3 cut(s)	171, 382, 995
TaaI	ACNGT	1 cut(s)	1337
TaiI	ACGT	5 cut(s)	432, 459, 786, 801, 1023
TaqI	TCGA	5 cut(s)	71, 242, 720, 792, 831
TasI	AATT	2 cut(s)	945, 1316
TatI	WGTACW	1 cut(s)	51
TfiI	GAWTC	4 cut(s)	176, 496, 1011, 1232
Tru1I	TTAA	3 cut(s)	15, 734, 1119
Tru9I	TTAA	3 cut(s)	15, 734, 1119
TscAI	CASTG	3 cut(s)	211, 886, 1049
TseFI	GTSAC	9 cut(s)	202, 335, 433, 565, 572, 685, 746, 1126, 1138
TseI	GCWGC	3 cut(s)	375, 547, 550
Tsp45I	GTSAC	9 cut(s)	202, 335, 433, 565, 572, 685, 746, 1126, 1138
TspDTI	ATGAA	4 cut(s)	117, 1088, 1096, 1226
TspGWI	ACGGA	1 cut(s)	1023
TspRI	CASTG	3 cut(s)	211, 886, 1049
Tth111I	GACNNNGTC	1 cut(s)	716
Van91I	CCANNNNNTGG	1 cut(s)	786
VpaK11BI	GGWCC	6 cut(s)	293, 419, 738, 1000, 1270, 1293
XagI	CCTNNNNNAGG	2 cut(s)	328, 471
XapI	RAATTY	1 cut(s)	1316
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1167
XmiI	GTMKAC	1 cut(s)	720
XspI	CTAG	3 cut(s)	570, 591, 1357
Zsp2I	ATGCAT	1 cut(s)	302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.