Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011725

Physical Location & Seq

Genomic Coordinates Reverse (-)

5463764 .. 5466089

Gene Structure 2326 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1078000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGGCAGCGATGTTTAACTCGTCCTATTTCTTTCTATGTTTGTTTTTCTTGTCCTTTGCTGTATTCGTTTCGAGCCACGATGATCCAACCGATGTTCCAAATGAAGATACGCAGGTATTAGATTTAGATGCGTACTGGAAACAACGAGCCGAAGAAGCTGAAAAGGAGGCCCTGGAATCCTACAATAAAAATCCTGAGCAAGTCACTGAAGAGTTCAATAATGAAGTTGGCAAGCTTATGTTAAAGGAAAACACCACAAGAAGGAACTTGAGGGGTAACAAGGCTTATAAGGGTCCATGCATGGCGACCAACCCAATTGACGCCTGCTGGAGGTGTGACCCTTATTGGGCTAACAACCGAAAGAAGATCGTAGGCTGCGCCCAAGGCTTTGGTAAAAAGGCCACCGGAGGTAAGGATGGTCCTTTTTACGTGGTAACAATGGGCACAGATGATGACGTGCAAAACCCTAAACCCGGAACTCTACGCCACGCCGTGATTCAGAAAGGGCCTCTGTGGATCATCTTCGCAAAAAGCATGGTGATTAGGCTGCAGCAAGAGCTGATGGTGACTAGTGACAAGACCATTGATGCTAGGGGAGCCAATGTTGTGATAGAAGAGGGTGCTGGCATTACCCTGCAGTTTGTGAAGAATGTAATCATCACCAACCTTCACATAAAAATGATTGTCCCCAAACCCGGTGGCATGATCAGGGACTCTGTCGACCACATAGGTTTAAGGACCCAGAGTGACGGCGATGGTATCTCCCTCTTTGGTGCCTCCAACGTCTGGATCGATCACGTTTCCATGTCCAGGTGCGCAGATGGGCTCATCGACGCCGTCATGGGTTCCACCTCCATCACCATCTCCAACTCCCACTTCACGGACCACGATGAGGCGATGTTGTTTGGTGCCAACAACGCCCACACTCAAGACAAAATCATGCAAATTACTTTGGCCTTCAACCACTTTGGTCAGGGACTGGTGCAGAGGATGCCAAGGGTCCGGCACGGATTCTTCCACGTGGTGAACAACGACTACACCCACTGGATCATGTATGCCATCGGAGGAAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTTCATTGCTCCCATTAACGGGGTCACCAAACAGGTGACCCATAGGGAGAATACACCAGAGGCAGAGTGGAAGAACTGGGAATGGAGATCTGAGGGAGATTTGATGATGAATGGAGCTTTCTTTGTTGAATCTGGATCAGGAGCCAGCAAACACCCAGCAAAGTTGGACATGATGCCCTTCAAACCAGGGACCTTTGTCGCAACGCTCACAAAATTTTCCGGTGCTCTAAACTGCGCTGTCGGCAAACCCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

453

Amino Acids

50.24

Weight (kDa)

6.54

Isoelectric Point (pI)

27.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	40 - 90	3.7e-19	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	187 - 379	3.9e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14420 AT2G02720
fragaria_vesca	FvH4_4g31900
malus_domestica	MD13G1078400.v1.1 MD16G1078000.v1.1 MD16G1078100.v1.1
prunus_persica	Prupe.1G268500_v2.0.a1 Prupe.1G268700_v2.0.a1
pyrus_communis	pycom13g06810 pycom16g06740 pycom16g06760
rosa_chinensis	RchiOBHm_Chr4g0440551
rosa_rugosa	Rorug04G0326600
rosa_samantha	Rh4AG378300 Rh4BG391000 Rh4CG405700 Rh4DG384700
rosa_wichuraiana	Rw4G032390 Rw4G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	288
Acc16I	TGCGCA	1 cut(s)	817
Acc36I	ACCTGC	1 cut(s)	103
AccB1I	GGYRCC	2 cut(s)	773, 908
AccB7I	CCANNNNNTGG	1 cut(s)	786
AccI	GTMKAC	1 cut(s)	720
AclWI	GGATC	5 cut(s)	77, 524, 797, 1055, 1246
AcsI	RAATTY	1 cut(s)	1316
AcuI	CTGAAG	1 cut(s)	228
AcvI	CACGTG	1 cut(s)	1021
AcyI	GRCGYC	2 cut(s)	321, 834
AdeI	CACNNNGTG	2 cut(s)	493, 1024
AfaI	GTAC	1 cut(s)	134
AgsI	TTSAA	4 cut(s)	217, 961, 1232, 1285
AhlI	ACTAGT	1 cut(s)	569
AjiI	CACGTC	1 cut(s)	457
AjnI	CCWGG	4 cut(s)	171, 809, 1092, 1288
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AluBI	AGCT	4 cut(s)	158, 235, 559, 1220
AluI	AGCT	4 cut(s)	158, 235, 559, 1220
Alw21I	GWGCWC	1 cut(s)	1330
AlwI	GGATC	5 cut(s)	77, 524, 797, 1055, 1246
AlwNI	CAGNNNCTG	1 cut(s)	979
AoxI	GGCC	4 cut(s)	168, 399, 506, 954
ApeKI	GCWGC	4 cut(s)	5, 375, 547, 550
ApoI	RAATTY	1 cut(s)	1316
AspLEI	GCGC	3 cut(s)	380, 818, 1340
AspS9I	GGNCC	8 cut(s)	169, 293, 419, 506, 738, 883, 1000, 1293
AsuC2I	CCSGG	2 cut(s)	474, 696
AsuHPI	GGTGA	7 cut(s)	550, 577, 652, 850, 1036, 1120, 1150
AvaII	GGWCC	6 cut(s)	293, 419, 738, 883, 1000, 1293
BaeGI	GKGCMC	1 cut(s)	446
BaeI	ACNNNNGTAYC	2 cut(s)	99, 132
BanI	GGYRCC	2 cut(s)	773, 908
BanII	GRGCYC	1 cut(s)	828
BbrPI	CACGTG	1 cut(s)	1021
Bbv12I	GWGCWC	1 cut(s)	1330
BbvI	GCAGC	4 cut(s)	17, 362, 534, 562
BccI	CCATC	8 cut(s)	410, 556, 749, 815, 863, 869, 1067, 1091
BceAI	ACGGC	3 cut(s)	476, 766, 821
BciT130I	CCWGG	4 cut(s)	173, 811, 1094, 1290
BclI	TGATCA	1 cut(s)	705
BcnI	CCSGG	2 cut(s)	474, 696
BcuI	ACTAGT	1 cut(s)	569
BfaI	CTAG	3 cut(s)	570, 591, 1357
BfmI	CTRYAG	2 cut(s)	548, 635
BfuAI	ACCTGC	1 cut(s)	103
BglII	AGATCT	1 cut(s)	1190
BisI	GCNGC	4 cut(s)	6, 376, 548, 551
BlsI	GCNGC	4 cut(s)	7, 377, 549, 552
Bme1390I	CCNGG	6 cut(s)	173, 474, 696, 811, 1094, 1290
Bme18I	GGWCC	6 cut(s)	293, 419, 738, 883, 1000, 1293
BmgBI	CACGTC	1 cut(s)	457
BmgT120I	GGNCC	8 cut(s)	169, 293, 419, 506, 738, 883, 1000, 1293
BmiI	GGNNCC	9 cut(s)	294, 598, 740, 775, 847, 910, 1001, 1246, 1294
BmrFI	CCNGG	6 cut(s)	173, 474, 696, 811, 1094, 1290
BmrI	ACTGGG	1 cut(s)	1189
BmsI	GCATC	4 cut(s)	118, 577, 981, 1266
BmuI	ACTGGG	1 cut(s)	1189
BoxI	GACNNNNGTC	1 cut(s)	1298
BpmI	CTGGAG	1 cut(s)	349
Bpu10I	CCTNAGC	1 cut(s)	195
BpuEI	CTTGAG	2 cut(s)	289, 912
BpuMI	CCSGG	2 cut(s)	474, 696
Bsa29I	ATCGAT	1 cut(s)	792
BsaAI	YACGTR	2 cut(s)	430, 1021
BsaHI	GRCGYC	2 cut(s)	321, 834
BsaJI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BsaWI	WCCGGW	2 cut(s)	404, 1322
BsaXI	ACNNNNNCTCC	2 cut(s)	588, 618
Bse1I	ACTGG	4 cut(s)	140, 984, 1049, 1184
Bse3DI	GCAATG	1 cut(s)	1107
BseBI	CCWGG	4 cut(s)	173, 811, 1094, 1290
BseCI	ATCGAT	1 cut(s)	792
BseDI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BseGI	GGATG	2 cut(s)	421, 996
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	2 cut(s)	186, 1185
BseNI	ACTGG	4 cut(s)	140, 984, 1049, 1184
BseSI	GKGCMC	1 cut(s)	446
BseXI	GCAGC	4 cut(s)	17, 362, 534, 562
BseYI	CCCAGC	1 cut(s)	1258
BsgI	GTGCAG	1 cut(s)	1004
BshFI	GGCC	4 cut(s)	170, 401, 508, 956
BshNI	GGYRCC	2 cut(s)	773, 908
BshVI	ATCGAT	1 cut(s)	792
BsiHKAI	GWGCWC	1 cut(s)	1330
BsiSI	CCGG	5 cut(s)	405, 474, 696, 1003, 1323
BslFI	GGGAC	4 cut(s)	671, 725, 990, 1306
BsmFI	GGGAC	4 cut(s)	671, 725, 990, 1306
BsnI	GGCC	4 cut(s)	170, 401, 508, 956
Bsp1286I	GDGCHC	3 cut(s)	446, 828, 1330
Bsp143I	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
BspANI	GGCC	4 cut(s)	170, 401, 508, 956
BspCNI	CTCAG	2 cut(s)	187, 1186
BspDI	ATCGAT	1 cut(s)	792
BspLI	GGNNCC	9 cut(s)	294, 598, 740, 775, 847, 910, 1001, 1246, 1294
BspMAI	CTGCAG	2 cut(s)	552, 639
BspMI	ACCTGC	1 cut(s)	103
BspPI	GGATC	5 cut(s)	77, 524, 797, 1055, 1246
BspT107I	GGYRCC	2 cut(s)	773, 908
BsrDI	GCAATG	1 cut(s)	1107
BsrI	ACTGG	4 cut(s)	140, 984, 1049, 1184
BssECI	CCNNGG	5 cut(s)	171, 382, 995, 1093, 1289
BssMI	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
BssNI	GRCGYC	2 cut(s)	321, 834
BssT1I	CCWWGG	2 cut(s)	382, 995
Bst2UI	CCWGG	4 cut(s)	173, 811, 1094, 1290
Bst6I	CTCTTC	2 cut(s)	204, 609
BstACI	GRCGYC	2 cut(s)	321, 834
BstAPI	GCANNNNNTGC	2 cut(s)	991, 1353
BstBAI	YACGTR	2 cut(s)	430, 1021
BstC8I	GCNNGC	4 cut(s)	233, 325, 625, 1249
BstDEI	CTNAG	2 cut(s)	195, 1194
BstEII	GGTNACC	2 cut(s)	1126, 1138
BstENI	CCTNNNNNAGG	1 cut(s)	328
BstF5I	GGATG	2 cut(s)	421, 996
BstHHI	GCGC	3 cut(s)	380, 818, 1340
BstKTI	GATC	9 cut(s)	85, 369, 519, 708, 792, 796, 1050, 1193, 1241
BstMBI	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
BstMWI	GCNNNNNNNGC	8 cut(s)	155, 384, 556, 596, 917, 991, 1344, 1353
BstNI	CCWGG	4 cut(s)	173, 811, 1094, 1290
BstPAI	GACNNNNGTC	1 cut(s)	1298
BstPI	GGTNACC	2 cut(s)	1126, 1138
BstSCI	CCNGG	6 cut(s)	171, 472, 694, 809, 1092, 1288
BstSFI	CTRYAG	2 cut(s)	548, 635
BstSLI	GKGCMC	1 cut(s)	446
BstV1I	GCAGC	4 cut(s)	17, 362, 534, 562
BstX2I	RGATCY	1 cut(s)	1190
BstXI	CCANNNNNNTGG	1 cut(s)	389
BstYI	RGATCY	1 cut(s)	1190
Bsu15I	ATCGAT	1 cut(s)	792
BsuRI	GGCC	4 cut(s)	170, 401, 508, 956
BsuTUI	ATCGAT	1 cut(s)	792
BtgZI	GCGATG	3 cut(s)	23, 768, 911
BtrI	CACGTC	1 cut(s)	457
BtsCI	GGATG	2 cut(s)	421, 996
BtsIMutI	CAGTG	2 cut(s)	204, 1042
BveI	ACCTGC	1 cut(s)	103
Cac8I	GCNNGC	4 cut(s)	233, 325, 625, 1249
CaiI	CAGNNNCTG	1 cut(s)	979
CfoI	GCGC	3 cut(s)	380, 818, 1340
Cfr13I	GGNCC	8 cut(s)	169, 293, 419, 506, 738, 883, 1000, 1293
ClaI	ATCGAT	1 cut(s)	792
CseI	GACGC	2 cut(s)	329, 842
Csp6I	GTAC	1 cut(s)	133
CspCI	CAANNNNNGTGG	2 cut(s)	679, 714
CviQI	GTAC	1 cut(s)	133
DdeI	CTNAG	2 cut(s)	195, 1194
DpnI	GATC	9 cut(s)	84, 368, 518, 707, 791, 795, 1049, 1192, 1240
DpnII	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
DraIII	CACNNNGTG	2 cut(s)	493, 1024
Eam1104I	CTCTTC	2 cut(s)	204, 609
EarI	CTCTTC	2 cut(s)	204, 609
Eco130I	CCWWGG	2 cut(s)	382, 995
Eco24I	GRGCYC	1 cut(s)	828
Eco47I	GGWCC	6 cut(s)	293, 419, 738, 883, 1000, 1293
Eco57I	CTGAAG	1 cut(s)	228
Eco72I	CACGTG	1 cut(s)	1021
Eco91I	GGTNACC	2 cut(s)	1126, 1138
EcoNI	CCTNNNNNAGG	1 cut(s)	328
EcoO109I	RGGNCCY	4 cut(s)	169, 506, 738, 1293
EcoO65I	GGTNACC	2 cut(s)	1126, 1138
EcoRII	CCWGG	4 cut(s)	171, 809, 1092, 1288
EcoT14I	CCWWGG	2 cut(s)	382, 995
EcoT22I	ATGCAT	1 cut(s)	302
EcoT38I	GRGCYC	1 cut(s)	828
ErhI	CCWWGG	2 cut(s)	382, 995
FaqI	GGGAC	4 cut(s)	671, 725, 990, 1306
FbaI	TGATCA	1 cut(s)	705
FblI	GTMKAC	1 cut(s)	720
Fnu4HI	GCNGC	4 cut(s)	6, 376, 548, 551
FokI	GGATG	2 cut(s)	428, 1003
FriOI	GRGCYC	1 cut(s)	828
Fsp4HI	GCNGC	4 cut(s)	6, 376, 548, 551
FspBI	CTAG	3 cut(s)	570, 591, 1357
FspI	TGCGCA	1 cut(s)	817
GlaI	GCGC	3 cut(s)	379, 817, 1339
GluI	GCNGC	4 cut(s)	6, 376, 548, 551
GsaI	CCCAGC	1 cut(s)	1262
GsuI	CTGGAG	1 cut(s)	349
HaeIII	GGCC	4 cut(s)	170, 401, 508, 956
HapII	CCGG	5 cut(s)	405, 474, 696, 1003, 1323
HgaI	GACGC	2 cut(s)	329, 842
HhaI	GCGC	3 cut(s)	380, 818, 1340
Hin1I	GRCGYC	2 cut(s)	321, 834
Hin6I	GCGC	3 cut(s)	378, 816, 1338
HinP1I	GCGC	3 cut(s)	378, 816, 1338
HincII	GTYRAC	1 cut(s)	721
HindII	GTYRAC	1 cut(s)	721
HindIII	AAGCTT	1 cut(s)	233
HinfI	GANTC	5 cut(s)	176, 496, 713, 1011, 1232
HpaII	CCGG	5 cut(s)	405, 474, 696, 1003, 1323
HphI	GGTGA	7 cut(s)	550, 577, 652, 850, 1036, 1120, 1150
Hpy166II	GTNNAC	2 cut(s)	721, 1027
Hpy188I	TCNGA	3 cut(s)	501, 1064, 1195
Hpy188III	TCNNGA	5 cut(s)	194, 787, 929, 1236, 1242
Hpy8I	GTNNAC	2 cut(s)	721, 1027
Hpy99I	CGWCG	1 cut(s)	836
HpyAV	CCTTC	4 cut(s)	255, 677, 967, 1291
HpyCH4IV	ACGT	5 cut(s)	429, 456, 783, 798, 1020
HpyCH4V	TGCA	6 cut(s)	300, 460, 550, 637, 943, 985
HpyF10VI	GCNNNNNNNGC	8 cut(s)	155, 384, 556, 596, 917, 991, 1344, 1353
HpyF3I	CTNAG	2 cut(s)	195, 1194
HpySE526I	ACGT	5 cut(s)	429, 456, 783, 798, 1020
Hsp92I	GRCGYC	2 cut(s)	321, 834
HspAI	GCGC	3 cut(s)	378, 816, 1338
Ksp22I	TGATCA	1 cut(s)	705
Kzo9I	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
LmnI	GCTCC	4 cut(s)	596, 1117, 1217, 1244
Lsp1109I	GCAGC	4 cut(s)	17, 362, 534, 562
LweI	GCATC	4 cut(s)	118, 577, 981, 1266
MaeI	CTAG	3 cut(s)	570, 591, 1357
MaeII	ACGT	5 cut(s)	429, 456, 783, 798, 1020
MalI	GATC	9 cut(s)	84, 368, 518, 707, 791, 795, 1049, 1192, 1240
MboI	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
MboII	GAAGA	9 cut(s)	116, 164, 221, 376, 514, 626, 658, 1006, 1186
MfeI	CAATTG	1 cut(s)	315
MflI	RGATCY	1 cut(s)	1190
MhlI	GDGCHC	3 cut(s)	446, 828, 1330
MluCI	AATT	3 cut(s)	315, 945, 1316
MlyI	GAGTC	1 cut(s)	707
MmeI	TCCRAC	4 cut(s)	110, 804, 891, 1248
Mph1103I	ATGCAT	1 cut(s)	302
MseI	TTAA	4 cut(s)	15, 242, 734, 1119
MslI	CAYNNNNRTG	1 cut(s)	677
MspI	CCGG	5 cut(s)	405, 474, 696, 1003, 1323
MspR9I	CCNGG	6 cut(s)	173, 474, 696, 811, 1094, 1290
MunI	CAATTG	1 cut(s)	315
MvaI	CCWGG	4 cut(s)	173, 811, 1094, 1290
MwoI	GCNNNNNNNGC	8 cut(s)	155, 384, 556, 596, 917, 991, 1344, 1353
NciI	CCSGG	2 cut(s)	474, 696
NdeII	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
NlaIV	GGNNCC	9 cut(s)	294, 598, 740, 775, 847, 910, 1001, 1246, 1294
NmuCI	GTSAC	7 cut(s)	202, 335, 565, 572, 746, 1126, 1138
NsbI	TGCGCA	1 cut(s)	817
NsiI	ATGCAT	1 cut(s)	302
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PfeI	GAWTC	4 cut(s)	176, 496, 1011, 1232
PflFI	GACNNNGTC	2 cut(s)	716, 836
PflMI	CCANNNNNTGG	1 cut(s)	786
PkrI	GCNGC	4 cut(s)	7, 377, 549, 552
PleI	GAGTC	1 cut(s)	707
PmaCI	CACGTG	1 cut(s)	1021
PmlI	CACGTG	1 cut(s)	1021
PpsI	GAGTC	1 cut(s)	707
Ppu21I	YACGTR	2 cut(s)	430, 1021
PpuMI	RGGWCCY	2 cut(s)	738, 1293
PshAI	GACNNNNGTC	1 cut(s)	1298
PsiI	TTATAA	1 cut(s)	288
Psp5II	RGGWCCY	2 cut(s)	738, 1293
Psp6I	CCWGG	4 cut(s)	171, 809, 1092, 1288
PspCI	CACGTG	1 cut(s)	1021
PspEI	GGTNACC	2 cut(s)	1126, 1138
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	4 cut(s)	171, 809, 1092, 1288
PspN4I	GGNNCC	9 cut(s)	294, 598, 740, 775, 847, 910, 1001, 1246, 1294
PspPI	GGNCC	8 cut(s)	169, 293, 419, 506, 738, 883, 1000, 1293
PspPPI	RGGWCCY	2 cut(s)	738, 1293
PsrI	GAACNNNNNNTAC	2 cut(s)	1019, 1051
PstI	CTGCAG	2 cut(s)	552, 639
PstNI	CAGNNNCTG	1 cut(s)	979
PsuI	RGATCY	1 cut(s)	1190
PsyI	GACNNNGTC	2 cut(s)	716, 836
RsaI	GTAC	1 cut(s)	134
RsaNI	GTAC	1 cut(s)	133
RseI	CAYNNNNRTG	1 cut(s)	677
SalI	GTCGAC	1 cut(s)	719
SaqAI	TTAA	4 cut(s)	15, 242, 734, 1119
SatI	GCNGC	4 cut(s)	6, 376, 548, 551
Sau3AI	GATC	9 cut(s)	82, 366, 516, 705, 789, 793, 1047, 1190, 1238
Sau96I	GGNCC	8 cut(s)	169, 293, 419, 506, 738, 883, 1000, 1293
SchI	GAGTC	1 cut(s)	707
ScrFI	CCNGG	6 cut(s)	173, 474, 696, 811, 1094, 1290
SduI	GDGCHC	3 cut(s)	446, 828, 1330
SfaNI	GCATC	4 cut(s)	118, 577, 981, 1266
SfcI	CTRYAG	2 cut(s)	548, 635
SinI	GGWCC	6 cut(s)	293, 419, 738, 883, 1000, 1293
SmiMI	CAYNNNNRTG	1 cut(s)	677
SmlI	CTYRAG	2 cut(s)	268, 927
SmoI	CTYRAG	2 cut(s)	268, 927
SpeI	ACTAGT	1 cut(s)	569
Sse9I	AATT	3 cut(s)	315, 945, 1316
SspMI	CTAG	3 cut(s)	570, 591, 1357
StyD4I	CCNGG	6 cut(s)	171, 472, 694, 809, 1092, 1288
StyI	CCWWGG	2 cut(s)	382, 995
TaiI	ACGT	5 cut(s)	432, 459, 786, 801, 1023
TaqI	TCGA	4 cut(s)	71, 720, 792, 831
TasI	AATT	3 cut(s)	315, 945, 1316
TfiI	GAWTC	4 cut(s)	176, 496, 1011, 1232
Tru1I	TTAA	4 cut(s)	15, 242, 734, 1119
Tru9I	TTAA	4 cut(s)	15, 242, 734, 1119
TscAI	CASTG	2 cut(s)	211, 1049
TseFI	GTSAC	7 cut(s)	202, 335, 565, 572, 746, 1126, 1138
TseI	GCWGC	4 cut(s)	5, 375, 547, 550
Tsp45I	GTSAC	7 cut(s)	202, 335, 565, 572, 746, 1126, 1138
TspDTI	ATGAA	5 cut(s)	117, 237, 1088, 1096, 1226
TspGWI	ACGGA	2 cut(s)	896, 1023
TspRI	CASTG	2 cut(s)	211, 1049
Tth111I	GACNNNGTC	2 cut(s)	716, 836
Van91I	CCANNNNNTGG	1 cut(s)	786
VpaK11BI	GGWCC	6 cut(s)	293, 419, 738, 883, 1000, 1293
XagI	CCTNNNNNAGG	1 cut(s)	328
XapI	RAATTY	1 cut(s)	1316
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1167
XmiI	GTMKAC	1 cut(s)	720
XspI	CTAG	3 cut(s)	570, 591, 1357
Zsp2I	ATGCAT	1 cut(s)	302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.