Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0011725

Physical Location & Seq

Genomic Coordinates Reverse (-)

59596128 .. 59598353

Gene Structure 2226 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G032490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGTCACAGACAATGGCACTGAATAGACTCGGTTCAATCTTTCTCGTCTTCTTTTTGTTGTCCTGTGTTGCTGTAATTGTCAACGCCGAAAATGATACTGCGGAAGATGAGTACTGGAAAGAACGAGCCGCTGTTGCTATGAAAATTGCCGAGGAATCTGTTCATCCAAATCCCCAGGAAGTTACCGCGGAGATCAATGCTGACGTACACGAGCTTATCATGGGGCAAAATAGCACGAAAAGGAAATTGAGAGGTTCCAAGGGATATGACGGTCCCTGCGAGGCCACCAACCCAATCGACGCATGCTGGCGGTGTGACCCTGATTGGGCCGACAATCGGGAGAAGATCGTCGATTGCGTCAAGGGTTTCGGCAAAAAGACTACCGGCGGCAAGGGTGGGCCCATCTACATTGTCACTGATGACTCAGATGACGACATTGTGAACCCTAAACCAGGAACCCTCCGCGACGCAGTGATCCAGAAAGGTCCTCTATGGATCATCTTTGAGCATAGCATGGTGATTCGTCTGCAACAAGAGCTGATGGTAACAAGTGACAAAACCATCGATGGTAGAGGAGCCAATGTTGTGATTGCCTTTGGTGGTGGCATCACTCTTCAGTATGTGAAAAATATCATCATCACGAACATTCACGTTAAGGACATTGAGTCCAAAGCCGGTGGTATGATCAGAGACTGCGTGAATCATTTTGGTTTTAGGACCAGGAGCGATGGCGATGGCATCTCCCTCTTCGGCGCTTCCAACGTCTGGATAGACCATGTTTCCATGGCGAGGTGCGACGACGGACTCATTGACGCTATCATGAGTTCCACTGCTATCACCATCTCCAACTCCCACTTCACTGACCACAACGAGGCAATGTTGTTCGGGGCAAGTAATAGCTACTCTCAAGACAAGATCATGCAAGTAACGGTCGCGTTCAACCACTTTGGGCAGGGATTGATTCAGAGAATGCCAAGGTGCCGATTTGGATTCTTCCACGTGGTGAACAATGACTACACCCATTGGATAATGTATGCTATTGGTGGTAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTACATTGCTCCTGAAAGCCATGTCGCAAAAGAGGTGACCAAGAGGGAGTACACAGACGAGTCGGAATGGCAGCACTGGAATTGGAGATCCGAGGGAGACTTGATGGTGAATGGAGCATATTTCGTCGAATCCGGGGACCCCAACGTTGCGAAATCCGAGAAGATAGAAATGTTGCCGTTCAAGCCCGGGACATTGGCCTCAACCCTTACTAGATTTTCCGGCGCTCTAGAGTGCTGTGTTGGCCAACCATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0016829 GO:0016835 GO:0016837 GO:0030570 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

445

Amino Acids

49.35

Weight (kDa)

5.41

Isoelectric Point (pI)

28.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	36 - 83	4.5e-13	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	178 - 380	3.3e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14420 AT2G02720
fragaria_vesca	FvH4_4g31900
malus_domestica	MD13G1078400.v1.1 MD16G1078000.v1.1 MD16G1078100.v1.1
prunus_persica	Prupe.1G268500_v2.0.a1 Prupe.1G268700_v2.0.a1
pyrus_communis	pycom13g06810 pycom16g06740 pycom16g06760
rosa_chinensis	RchiOBHm_Chr4g0440551
rosa_rugosa	Rorug04G0326600
rosa_samantha	Rh4AG378300 Rh4BG391000 Rh4CG405700 Rh4DG384700
rosa_wichuraiana	Rw4G032390 Rw4G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	978
AccB7I	CCANNNNNTGG	1 cut(s)	765
AccII	CGCG	3 cut(s)	188, 465, 935
AciI	CCGC	7 cut(s)	101, 129, 186, 188, 310, 387, 463
AclI	AACGTT	1 cut(s)	1227
AclWI	GGATC	3 cut(s)	469, 503, 1164
AcoI	YGGCCR	1 cut(s)	1324
AcuI	CTGAAG	1 cut(s)	599
AcvI	CACGTG	1 cut(s)	1000
AdeI	CACNNNGTG	1 cut(s)	1003
AfaI	GTAC	4 cut(s)	113, 207, 1085, 1133
AfiI	CCNNNNNNNGG	6 cut(s)	325, 336, 452, 765, 789, 871
AgsI	TTSAA	3 cut(s)	36, 940, 1264
AhdI	GACNNNNNGTC	1 cut(s)	664
AjnI	CCWGG	4 cut(s)	174, 451, 719, 1071
AjuI	GAANNNNNNNTTGG	2 cut(s)	839, 871
AluBI	AGCT	3 cut(s)	214, 538, 900
AluI	AGCT	3 cut(s)	214, 538, 900
Alw26I	GTCTC	2 cut(s)	684, 1173
AlwI	GGATC	3 cut(s)	469, 503, 1164
AlwNI	CAGNNNCTG	1 cut(s)	693
Ama87I	CYCGRG	1 cut(s)	1268
AoxI	GGCC	5 cut(s)	282, 327, 398, 1278, 1324
ApaI	GGGCCC	1 cut(s)	402
ApeKI	GCWGC	1 cut(s)	1153
Asp700I	GAANNNNTTC	1 cut(s)	159
AspLEI	GCGC	2 cut(s)	755, 1307
AspS9I	GGNCC	7 cut(s)	272, 327, 398, 399, 485, 717, 1219
AsuC2I	CCSGG	3 cut(s)	1216, 1269, 1270
AsuHPI	GGTGA	5 cut(s)	529, 829, 1015, 1129, 1201
AvaI	CYCGRG	1 cut(s)	1268
AvaII	GGWCC	4 cut(s)	272, 485, 717, 1219
BaeGI	GKGCMC	1 cut(s)	402
BalI	TGGCCA	1 cut(s)	1326
BanI	GGYRCC	1 cut(s)	978
BanII	GRGCYC	1 cut(s)	402
BauI	CACGAG	1 cut(s)	209
BbrPI	CACGTG	1 cut(s)	1000
BbsI	GAAGAC	1 cut(s)	40
BbvI	GCAGC	1 cut(s)	1165
BccI	CCATC	9 cut(s)	410, 535, 560, 569, 722, 728, 848, 1070, 1180
BceAI	ACGGC	1 cut(s)	1243
BciT130I	CCWGG	4 cut(s)	176, 453, 721, 1073
BclI	TGATCA	1 cut(s)	684
BcnI	CCSGG	3 cut(s)	1216, 1269, 1270
BcoDI	GTCTC	2 cut(s)	684, 1173
BfaI	CTAG	3 cut(s)	1293, 1310, 1336
BfoI	RGCGCY	2 cut(s)	756, 1308
BisI	GCNGC	3 cut(s)	129, 388, 1154
BlsI	GCNGC	3 cut(s)	130, 389, 1155
BmcAI	AGTACT	1 cut(s)	113
Bme1390I	CCNGG	7 cut(s)	176, 453, 721, 1073, 1216, 1269, 1270
Bme18I	GGWCC	4 cut(s)	272, 485, 717, 1219
BmeRI	GACNNNNNGTC	1 cut(s)	664
BmeT110I	CYCGRG	1 cut(s)	1268
BmgT120I	GGNCC	7 cut(s)	272, 327, 398, 399, 485, 717, 1219
BmiI	GGNNCC	8 cut(s)	256, 274, 400, 457, 577, 980, 1220, 1221
BmrFI	CCNGG	7 cut(s)	176, 453, 721, 1073, 1216, 1269, 1270
BmsI	GCATC	2 cut(s)	615, 747
BpiI	GAAGAC	1 cut(s)	40
BpuEI	CTTGAG	1 cut(s)	891
BpuMI	CCSGG	3 cut(s)	1216, 1269, 1270
Bsa29I	ATCGAT	1 cut(s)	564
BsaAI	YACGTR	1 cut(s)	1000
BsaXI	ACNNNNNCTCC	2 cut(s)	567, 597
Bsc4I	CCNNNNNNNGG	6 cut(s)	325, 336, 452, 765, 789, 871
Bse118I	RCCGGY	2 cut(s)	383, 674
Bse1I	ACTGG	2 cut(s)	119, 1163
Bse3DI	GCAATG	2 cut(s)	882, 1086
BseBI	CCWGG	4 cut(s)	176, 453, 721, 1073
BseCI	ATCGAT	1 cut(s)	564
BseGI	GGATG	1 cut(s)	163
BseLI	CCNNNNNNNGG	6 cut(s)	325, 336, 452, 765, 789, 871
BseMI	GCAATG	2 cut(s)	882, 1086
BseMII	CTCAG	1 cut(s)	438
BseNI	ACTGG	2 cut(s)	119, 1163
BseRI	GAGGAG	1 cut(s)	588
BseSI	GKGCMC	1 cut(s)	402
BseXI	GCAGC	1 cut(s)	1165
Bsh1236I	CGCG	3 cut(s)	188, 465, 935
Bsh1285I	CGRYCG	1 cut(s)	933
BshFI	GGCC	5 cut(s)	284, 329, 400, 1280, 1326
BshNI	GGYRCC	1 cut(s)	978
BshVI	ATCGAT	1 cut(s)	564
BsiEI	CGRYCG	1 cut(s)	933
BsiHKCI	CYCGRG	1 cut(s)	1268
BsiSI	CCGG	5 cut(s)	384, 675, 1215, 1269, 1302
BslFI	GGGAC	3 cut(s)	258, 1232, 1285
BslI	CCNNNNNNNGG	6 cut(s)	325, 336, 452, 765, 789, 871
BsmAI	GTCTC	2 cut(s)	684, 1173
BsmFI	GGGAC	3 cut(s)	258, 1232, 1285
BsmI	GAATGC	1 cut(s)	975
BsnI	GGCC	5 cut(s)	284, 329, 400, 1280, 1326
BsoBI	CYCGRG	1 cut(s)	1268
Bsp120I	GGGCCC	1 cut(s)	398
Bsp1286I	GDGCHC	1 cut(s)	402
Bsp143I	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
Bsp19I	CCATGG	1 cut(s)	783
BspACI	CCGC	7 cut(s)	101, 129, 186, 188, 310, 387, 463
BspANI	GGCC	5 cut(s)	284, 329, 400, 1280, 1326
BspCNI	CTCAG	1 cut(s)	437
BspDI	ATCGAT	1 cut(s)	564
BspFNI	CGCG	3 cut(s)	188, 465, 935
BspHI	TCATGA	1 cut(s)	819
BspLI	GGNNCC	8 cut(s)	256, 274, 400, 457, 577, 980, 1220, 1221
BspPI	GGATC	3 cut(s)	469, 503, 1164
BspT107I	GGYRCC	1 cut(s)	978
BsrDI	GCAATG	2 cut(s)	882, 1086
BsrFI	RCCGGY	2 cut(s)	383, 674
BsrI	ACTGG	2 cut(s)	119, 1163
BssAI	RCCGGY	2 cut(s)	383, 674
BssMI	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
BssSI	CACGAG	1 cut(s)	209
BssT1I	CCWWGG	3 cut(s)	258, 783, 974
Bst2BI	CACGAG	1 cut(s)	209
Bst2UI	CCWGG	4 cut(s)	176, 453, 721, 1073
Bst4CI	ACNGT	2 cut(s)	272, 931
Bst6I	CTCTTC	2 cut(s)	618, 752
BstBAI	YACGTR	1 cut(s)	1000
BstC8I	GCNNGC	2 cut(s)	304, 308
BstDEI	CTNAG	1 cut(s)	424
BstDSI	CCRYGG	2 cut(s)	186, 783
BstEII	GGTNACC	1 cut(s)	1117
BstENI	CCTNNNNNAGG	1 cut(s)	450
BstF5I	GGATG	1 cut(s)	163
BstFNI	CGCG	3 cut(s)	188, 465, 935
BstH2I	RGCGCY	2 cut(s)	756, 1308
BstHHI	GCGC	2 cut(s)	755, 1307
BstKTI	GATC	7 cut(s)	195, 348, 477, 498, 687, 918, 1172
BstMAI	GTCTC	2 cut(s)	684, 1173
BstMBI	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
BstMCI	CGRYCG	1 cut(s)	933
BstMWI	GCNNNNNNNGC	5 cut(s)	134, 535, 1264, 1323, 1332
BstNI	CCWGG	4 cut(s)	176, 453, 721, 1073
BstNSI	RCATGY	1 cut(s)	306
BstPI	GGTNACC	1 cut(s)	1117
BstSCI	CCNGG	7 cut(s)	174, 451, 719, 1071, 1214, 1267, 1268
BstSLI	GKGCMC	1 cut(s)	402
BstUI	CGCG	3 cut(s)	188, 465, 935
BstV1I	GCAGC	1 cut(s)	1165
BstV2I	GAAGAC	1 cut(s)	40
BstX2I	RGATCY	1 cut(s)	1169
BstYI	RGATCY	1 cut(s)	1169
Bsu15I	ATCGAT	1 cut(s)	564
BsuRI	GGCC	5 cut(s)	284, 329, 400, 1280, 1326
BsuTUI	ATCGAT	1 cut(s)	564
BtgI	CCRYGG	2 cut(s)	186, 783
BtgZI	GCGATG	2 cut(s)	741, 747
BtsCI	GGATG	1 cut(s)	163
BtsI	GCAGTG	2 cut(s)	477, 828
BtsIMutI	CAGTG	6 cut(s)	17, 414, 477, 828, 858, 1156
Cac8I	GCNNGC	2 cut(s)	304, 308
CaiI	CAGNNNCTG	1 cut(s)	693
CciI	TCATGA	1 cut(s)	819
CfoI	GCGC	2 cut(s)	755, 1307
Cfr10I	RCCGGY	2 cut(s)	383, 674
Cfr13I	GGNCC	7 cut(s)	272, 327, 398, 399, 485, 717, 1219
Cfr42I	CCGCGG	1 cut(s)	189
Cfr9I	CCCGGG	1 cut(s)	1268
ClaI	ATCGAT	1 cut(s)	564
CseI	GACGC	4 cut(s)	308, 346, 476, 821
Csp6I	GTAC	4 cut(s)	112, 206, 1084, 1132
CspCI	CAANNNNNGTGG	2 cut(s)	658, 693
CviQI	GTAC	4 cut(s)	112, 206, 1084, 1132
DdeI	CTNAG	1 cut(s)	424
DpnI	GATC	7 cut(s)	194, 347, 476, 497, 686, 917, 1171
DpnII	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
DraIII	CACNNNGTG	1 cut(s)	1003
DriI	GACNNNNNGTC	1 cut(s)	664
EaeI	YGGCCR	1 cut(s)	1324
Eam1104I	CTCTTC	2 cut(s)	618, 752
Eam1105I	GACNNNNNGTC	1 cut(s)	664
EarI	CTCTTC	2 cut(s)	618, 752
Eco130I	CCWWGG	3 cut(s)	258, 783, 974
Eco24I	GRGCYC	1 cut(s)	402
Eco47I	GGWCC	4 cut(s)	272, 485, 717, 1219
Eco57I	CTGAAG	1 cut(s)	599
Eco72I	CACGTG	1 cut(s)	1000
Eco88I	CYCGRG	1 cut(s)	1268
Eco91I	GGTNACC	1 cut(s)	1117
EcoNI	CCTNNNNNAGG	1 cut(s)	450
EcoO109I	RGGNCCY	2 cut(s)	485, 1219
EcoO65I	GGTNACC	1 cut(s)	1117
EcoRII	CCWGG	4 cut(s)	174, 451, 719, 1071
EcoT14I	CCWWGG	3 cut(s)	258, 783, 974
EcoT38I	GRGCYC	1 cut(s)	402
ErhI	CCWWGG	3 cut(s)	258, 783, 974
FaqI	GGGAC	3 cut(s)	258, 1232, 1285
FbaI	TGATCA	1 cut(s)	684
Fnu4HI	GCNGC	3 cut(s)	129, 388, 1154
FokI	GGATG	1 cut(s)	150
FriOI	GRGCYC	1 cut(s)	402
Fsp4HI	GCNGC	3 cut(s)	129, 388, 1154
FspBI	CTAG	3 cut(s)	1293, 1310, 1336
GlaI	GCGC	2 cut(s)	754, 1306
GluI	GCNGC	3 cut(s)	129, 388, 1154
HaeII	RGCGCY	2 cut(s)	756, 1308
HaeIII	GGCC	5 cut(s)	284, 329, 400, 1280, 1326
HapII	CCGG	5 cut(s)	384, 675, 1215, 1269, 1302
HgaI	GACGC	4 cut(s)	308, 346, 476, 821
HhaI	GCGC	2 cut(s)	755, 1307
Hin6I	GCGC	2 cut(s)	753, 1305
HinP1I	GCGC	2 cut(s)	753, 1305
HincII	GTYRAC	1 cut(s)	82
HindII	GTYRAC	1 cut(s)	82
HpaII	CCGG	5 cut(s)	384, 675, 1215, 1269, 1302
HphI	GGTGA	5 cut(s)	529, 829, 1015, 1129, 1201
Hpy166II	GTNNAC	5 cut(s)	82, 208, 442, 1006, 1134
Hpy188I	TCNGA	6 cut(s)	427, 689, 966, 1147, 1174, 1240
Hpy188III	TCNNGA	8 cut(s)	338, 478, 640, 766, 820, 908, 1094, 1310
Hpy8I	GTNNAC	5 cut(s)	82, 208, 442, 1006, 1134
Hpy99I	CGWCG	6 cut(s)	302, 353, 470, 800, 803, 1211
HpyCH4III	ACNGT	2 cut(s)	272, 931
HpyCH4IV	ACGT	5 cut(s)	204, 651, 762, 999, 1227
HpyCH4V	TGCA	2 cut(s)	529, 922
HpyF10VI	GCNNNNNNNGC	5 cut(s)	134, 535, 1264, 1323, 1332
HpyF3I	CTNAG	1 cut(s)	424
HpySE526I	ACGT	5 cut(s)	204, 651, 762, 999, 1227
HspAI	GCGC	2 cut(s)	753, 1305
KflI	GGGWCCC	1 cut(s)	1219
Ksp22I	TGATCA	1 cut(s)	684
KspI	CCGCGG	1 cut(s)	189
Kzo9I	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
LmnI	GCTCC	4 cut(s)	575, 723, 1096, 1196
Lsp1109I	GCAGC	1 cut(s)	1165
LweI	GCATC	2 cut(s)	615, 747
MaeI	CTAG	3 cut(s)	1293, 1310, 1336
MaeII	ACGT	5 cut(s)	204, 651, 762, 999, 1227
MalI	GATC	7 cut(s)	194, 347, 476, 497, 686, 917, 1171
MboI	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
MboII	GAAGA	7 cut(s)	40, 116, 355, 605, 739, 985, 1255
MflI	RGATCY	1 cut(s)	1169
MhlI	GDGCHC	1 cut(s)	402
MlsI	TGGCCA	1 cut(s)	1326
MluCI	AATT	4 cut(s)	75, 144, 245, 1162
MluNI	TGGCCA	1 cut(s)	1326
MlyI	GAGTC	5 cut(s)	21, 416, 674, 798, 1151
MmeI	TCCRAC	3 cut(s)	783, 870, 1125
Mox20I	TGGCCA	1 cut(s)	1326
MroXI	GAANNNNTTC	1 cut(s)	159
MscI	TGGCCA	1 cut(s)	1326
MseI	TTAA	1 cut(s)	654
Msp20I	TGGCCA	1 cut(s)	1326
MspA1I	CMGCKG	2 cut(s)	131, 188
MspI	CCGG	5 cut(s)	384, 675, 1215, 1269, 1302
MspR9I	CCNGG	7 cut(s)	176, 453, 721, 1073, 1216, 1269, 1270
Mva1269I	GAATGC	1 cut(s)	975
MvaI	CCWGG	4 cut(s)	176, 453, 721, 1073
MvnI	CGCG	3 cut(s)	188, 465, 935
MwoI	GCNNNNNNNGC	5 cut(s)	134, 535, 1264, 1323, 1332
NciI	CCSGG	3 cut(s)	1216, 1269, 1270
NcoI	CCATGG	1 cut(s)	783
NdeII	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
NlaIV	GGNNCC	8 cut(s)	256, 274, 400, 457, 577, 980, 1220, 1221
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	5 cut(s)	3, 314, 412, 551, 1117
NspI	RCATGY	1 cut(s)	306
PaeI	GCATGC	1 cut(s)	306
PagI	TCATGA	1 cut(s)	819
PcsI	WCGNNNNNNNCGW	2 cut(s)	276, 354
PctI	GAATGC	1 cut(s)	975
PdmI	GAANNNNTTC	1 cut(s)	159
PfeI	GAWTC	6 cut(s)	155, 520, 700, 961, 990, 1211
PflMI	CCANNNNNTGG	1 cut(s)	765
PkrI	GCNGC	3 cut(s)	130, 389, 1155
PleI	GAGTC	5 cut(s)	21, 416, 673, 798, 1150
PmaCI	CACGTG	1 cut(s)	1000
PmlI	CACGTG	1 cut(s)	1000
PpsI	GAGTC	5 cut(s)	21, 416, 673, 798, 1150
Ppu21I	YACGTR	1 cut(s)	1000
PpuMI	RGGWCCY	2 cut(s)	485, 1219
Psp1406I	AACGTT	1 cut(s)	1227
Psp5II	RGGWCCY	2 cut(s)	485, 1219
Psp6I	CCWGG	4 cut(s)	174, 451, 719, 1071
PspCI	CACGTG	1 cut(s)	1000
PspEI	GGTNACC	1 cut(s)	1117
PspGI	CCWGG	4 cut(s)	174, 451, 719, 1071
PspN4I	GGNNCC	8 cut(s)	256, 274, 400, 457, 577, 980, 1220, 1221
PspOMI	GGGCCC	1 cut(s)	398
PspPI	GGNCC	7 cut(s)	272, 327, 398, 399, 485, 717, 1219
PspPPI	RGGWCCY	2 cut(s)	485, 1219
PsrI	GAACNNNNNNTAC	2 cut(s)	998, 1030
PstNI	CAGNNNCTG	1 cut(s)	693
PsuI	RGATCY	1 cut(s)	1169
RsaI	GTAC	4 cut(s)	113, 207, 1085, 1133
RsaNI	GTAC	4 cut(s)	112, 206, 1084, 1132
SacII	CCGCGG	1 cut(s)	189
SaqAI	TTAA	1 cut(s)	654
SatI	GCNGC	3 cut(s)	129, 388, 1154
Sau3AI	GATC	7 cut(s)	192, 345, 474, 495, 684, 915, 1169
Sau96I	GGNCC	7 cut(s)	272, 327, 398, 399, 485, 717, 1219
ScaI	AGTACT	1 cut(s)	113
SchI	GAGTC	5 cut(s)	21, 416, 674, 798, 1151
ScrFI	CCNGG	7 cut(s)	176, 453, 721, 1073, 1216, 1269, 1270
SduI	GDGCHC	1 cut(s)	402
SfaNI	GCATC	2 cut(s)	615, 747
Sfr303I	CCGCGG	1 cut(s)	189
SgrBI	CCGCGG	1 cut(s)	189
SinI	GGWCC	4 cut(s)	272, 485, 717, 1219
SmaI	CCCGGG	1 cut(s)	1270
SmlI	CTYRAG	1 cut(s)	906
SmoI	CTYRAG	1 cut(s)	906
SphI	GCATGC	1 cut(s)	306
Sse9I	AATT	4 cut(s)	75, 144, 245, 1162
SsiI	CCGC	7 cut(s)	101, 129, 186, 188, 310, 387, 463
SspMI	CTAG	3 cut(s)	1293, 1310, 1336
StyD4I	CCNGG	7 cut(s)	174, 451, 719, 1071, 1214, 1267, 1268
StyI	CCWWGG	3 cut(s)	258, 783, 974
TaaI	ACNGT	2 cut(s)	272, 931
TaiI	ACGT	5 cut(s)	207, 654, 765, 1002, 1230
TaqI	TCGA	4 cut(s)	297, 351, 564, 1209
TasI	AATT	4 cut(s)	75, 144, 245, 1162
TatI	WGTACW	2 cut(s)	111, 1131
TauI	GCSGC	2 cut(s)	131, 390
TfiI	GAWTC	6 cut(s)	155, 520, 700, 961, 990, 1211
Tru1I	TTAA	1 cut(s)	654
Tru9I	TTAA	1 cut(s)	654
TscAI	CASTG	6 cut(s)	24, 421, 477, 835, 865, 1163
TseFI	GTSAC	5 cut(s)	3, 314, 412, 551, 1117
TseI	GCWGC	1 cut(s)	1153
Tsp45I	GTSAC	5 cut(s)	3, 314, 412, 551, 1117
TspDTI	ATGAA	3 cut(s)	152, 155, 1067
TspGWI	ACGGA	1 cut(s)	816
TspMI	CCCGGG	1 cut(s)	1268
TspRI	CASTG	6 cut(s)	24, 421, 477, 835, 865, 1163
Van91I	CCANNNNNTGG	1 cut(s)	765
VpaK11BI	GGWCC	4 cut(s)	272, 485, 717, 1219
XagI	CCTNNNNNAGG	1 cut(s)	450
XbaI	TCTAGA	1 cut(s)	1309
XceI	RCATGY	1 cut(s)	306
XmaI	CCCGGG	1 cut(s)	1268
XmnI	GAANNNNTTC	1 cut(s)	159
XspI	CTAG	3 cut(s)	1293, 1310, 1336
ZrmI	AGTACT	1 cut(s)	113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.