Rh4AG378300

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011725
Physical Location & Seq
Reverse (-)
68529432 .. 68531702
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG378300.1

Sequence Viewer

Length: 930 bp
ATGCATGCATGCAGGCTTATCATGGGGCAAAATAGCACGAAAAGGAAATTGAGAGGTTCCAAGGGATATGACGGTCCCTGCGAGGCCACCAACCCAATCGACGCATGCTGGCGGTGTGACCCTGATTGGGCCGACAATCGGGAGAAGATCGTCGATTGCGTCAAGGGTTTCGGCAAAAAGACTACCGGCGGCAAGGGTGGGCCCATCTACATTGTCACTGATGACTCAGATGACGACATTGTGAACCCTAAACCAGGAACCCTCCGCGACGCAGTGATCCAGAAAGGTCCTCTATGGATCATCTTTGAGCATAGCATGGTGATTCGTCTGCAACAAGAGCTGATGGTAACAAGTGACAAAACCATCGATGGTAGAGGAGCCAATGTTGTGATTGCCTTTGGTGGTGGTATCACTCTTCAGTATGTGAAAAATATCATCATCACGAACATTCACGTTAAGGACATTGAGTCCAAAGCCGGTGGTATGATCAGAGACTGCGTGAATCATTTTGGTTTTAGGACCAGGAGCGATGGCGATGGCATCTCCCTCTTCGGCGCTTCCAACGTCTGGATCGACCATGTTTCCATGGCGAGGTGCGACGACGGACTCATTGACGCTATCATGAGTTCCACTGCTATCACCATCTCCAACTCCCACTTCACTGACCACAACGAGGCAATGTTGTTCGGGGCAAGTAATAGCTACTCTCAAGACAAGATCATGCAAGTAACGGTCGCGTTCAACCACTTTGGGCAGGGGTTGATTCAGAGAATGCCAAGATGCCGATTTGGATTCTTCCACGTGGTGAACAATGACTACACCCATTGGATAATGTATGCTATTGGTGGTAACATGAACCCTACCATCATCAGCCAGGGTAACAGGTACATTGCCCCTGAAAGCCATGTCGCAAAAGAGGTATGCAGTTAA

Protein Analysis

309

Amino Acids

34.14

Weight (kDa)

6.82

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 112 - 297 1.3e-19 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 567
AccII CGCG 2 cut(s) 267, 737
AciI CCGC 3 cut(s) 112, 189, 265
AclWI GGATC 3 cut(s) 271, 305, 578
AcuI CTGAAG 1 cut(s) 401
AcvI CACGTG 1 cut(s) 802
AdeI CACNNNGTG 1 cut(s) 805
AfaI GTAC 1 cut(s) 887
AfiI CCNNNNNNNGG 6 cut(s) 127, 138, 254, 567, 591, 673
AgsI TTSAA 1 cut(s) 742
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 3 cut(s) 253, 521, 873
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 2 cut(s) 340, 702
AluI AGCT 2 cut(s) 340, 702
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 3 cut(s) 271, 305, 578
AlwNI CAGNNNCTG 1 cut(s) 495
AoxI GGCC 3 cut(s) 84, 129, 200
ApaI GGGCCC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 6 cut(s) 74, 129, 200, 201, 287, 519
AsuHPI GGTGA 3 cut(s) 331, 631, 817
AvaII GGWCC 3 cut(s) 74, 287, 519
BaeGI GKGCMC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 204
BbrPI CACGTG 1 cut(s) 802
BccI CCATC 8 cut(s) 212, 337, 362, 371, 524, 530, 650, 872
BciT130I CCWGG 3 cut(s) 255, 523, 875
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 558
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 3 cut(s) 255, 523, 875
Bme18I GGWCC 3 cut(s) 74, 287, 519
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 6 cut(s) 74, 129, 200, 201, 287, 519
BmiI GGNNCC 5 cut(s) 58, 76, 202, 259, 379
BmrFI CCNGG 3 cut(s) 255, 523, 875
BmsI GCATC 2 cut(s) 549, 770
BpuEI CTTGAG 1 cut(s) 693
Bsa29I ATCGAT 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 802
BsaJI CCNNGG 3 cut(s) 60, 585, 874
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 138, 254, 567, 591, 673
Bse118I RCCGGY 2 cut(s) 185, 476
Bse3DI GCAATG 2 cut(s) 684, 888
BseBI CCWGG 3 cut(s) 255, 523, 875
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 3 cut(s) 60, 585, 874
BseLI CCNNNNNNNGG 6 cut(s) 127, 138, 254, 567, 591, 673
BseMI GCAATG 2 cut(s) 684, 888
BseMII CTCAG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 267, 737
Bsh1285I CGRYCG 1 cut(s) 735
BshFI GGCC 3 cut(s) 86, 131, 202
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 735
BsiSI CCGG 2 cut(s) 186, 477
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 6 cut(s) 127, 138, 254, 567, 591, 673
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 86, 131, 202
Bsp120I GGGCCC 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 6 cut(s) 147, 276, 297, 486, 570, 717
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 3 cut(s) 112, 189, 265
BspANI GGCC 3 cut(s) 86, 131, 202
BspCNI CTCAG 1 cut(s) 239
BspDI ATCGAT 1 cut(s) 366
BspFNI CGCG 2 cut(s) 267, 737
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 5 cut(s) 58, 76, 202, 259, 379
BspPI GGATC 3 cut(s) 271, 305, 578
BsrDI GCAATG 2 cut(s) 684, 888
BsrFI RCCGGY 2 cut(s) 185, 476
BssAI RCCGGY 2 cut(s) 185, 476
BssECI CCNNGG 3 cut(s) 60, 585, 874
BssMI GATC 6 cut(s) 147, 276, 297, 486, 570, 717
BssT1I CCWWGG 2 cut(s) 60, 585
Bst2UI CCWGG 3 cut(s) 255, 523, 875
Bst4CI ACNGT 2 cut(s) 74, 733
Bst6I CTCTTC 2 cut(s) 420, 554
BstBAI YACGTR 1 cut(s) 802
BstC8I GCNNGC 5 cut(s) 6, 10, 14, 106, 110
BstDEI CTNAG 1 cut(s) 226
BstDSI CCRYGG 1 cut(s) 585
BstENI CCTNNNNNAGG 1 cut(s) 252
BstFNI CGCG 2 cut(s) 267, 737
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 6 cut(s) 150, 279, 300, 489, 573, 720
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 6 cut(s) 147, 276, 297, 486, 570, 717
BstMCI CGRYCG 1 cut(s) 735
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstNI CCWGG 3 cut(s) 255, 523, 875
BstNSI RCATGY 3 cut(s) 8, 12, 108
BstSCI CCNGG 3 cut(s) 253, 521, 873
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 2 cut(s) 267, 737
Bsu15I ATCGAT 1 cut(s) 366
BsuRI GGCC 3 cut(s) 86, 131, 202
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 585
BtgZI GCGATG 2 cut(s) 543, 549
BtsI GCAGTG 2 cut(s) 279, 630
BtsIMutI CAGTG 4 cut(s) 216, 279, 630, 660
Cac8I GCNNGC 5 cut(s) 6, 10, 14, 106, 110
CaiI CAGNNNCTG 1 cut(s) 495
CciI TCATGA 1 cut(s) 621
CfoI GCGC 1 cut(s) 557
Cfr10I RCCGGY 2 cut(s) 185, 476
Cfr13I GGNCC 6 cut(s) 74, 129, 200, 201, 287, 519
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 4 cut(s) 110, 148, 278, 623
Csp6I GTAC 1 cut(s) 886
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviQI GTAC 1 cut(s) 886
DdeI CTNAG 1 cut(s) 226
DpnI GATC 6 cut(s) 149, 278, 299, 488, 572, 719
DpnII GATC 6 cut(s) 147, 276, 297, 486, 570, 717
DraIII CACNNNGTG 1 cut(s) 805
DriI GACNNNNNGTC 1 cut(s) 466
Eam1104I CTCTTC 2 cut(s) 420, 554
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 2 cut(s) 420, 554
Eco130I CCWWGG 2 cut(s) 60, 585
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 74, 287, 519
Eco57I CTGAAG 1 cut(s) 401
Eco72I CACGTG 1 cut(s) 802
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 3 cut(s) 253, 521, 873
EcoT14I CCWWGG 2 cut(s) 60, 585
EcoT22I ATGCAT 2 cut(s) 6, 10
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 2 cut(s) 60, 585
FaqI GGGAC 1 cut(s) 60
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 190
GlaI GCGC 1 cut(s) 556
GluI GCNGC 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 3 cut(s) 86, 131, 202
HapII CCGG 2 cut(s) 186, 477
HgaI GACGC 4 cut(s) 110, 148, 278, 623
HhaI GCGC 1 cut(s) 557
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HinfI GANTC 7 cut(s) 224, 322, 467, 502, 606, 763, 792
HpaII CCGG 2 cut(s) 186, 477
HphI GGTGA 3 cut(s) 331, 631, 817
Hpy166II GTNNAC 2 cut(s) 244, 808
Hpy188I TCNGA 3 cut(s) 229, 491, 768
Hpy188III TCNNGA 6 cut(s) 140, 280, 442, 568, 622, 710
Hpy8I GTNNAC 2 cut(s) 244, 808
Hpy99I CGWCG 5 cut(s) 104, 155, 272, 602, 605
HpyCH4III ACNGT 2 cut(s) 74, 733
HpyCH4IV ACGT 3 cut(s) 453, 564, 801
HpyCH4V TGCA 6 cut(s) 4, 8, 12, 331, 724, 924
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 1 cut(s) 226
HpySE526I ACGT 3 cut(s) 453, 564, 801
HspAI GCGC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 6 cut(s) 147, 276, 297, 486, 570, 717
LmnI GCTCC 2 cut(s) 377, 525
LweI GCATC 2 cut(s) 549, 770
MaeII ACGT 3 cut(s) 453, 564, 801
MaeIII GTNAC 7 cut(s) 116, 214, 346, 353, 727, 848, 878
MalI GATC 6 cut(s) 149, 278, 299, 488, 572, 719
MboI GATC 6 cut(s) 147, 276, 297, 486, 570, 717
MboII GAAGA 4 cut(s) 157, 407, 541, 787
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 47
MlyI GAGTC 3 cut(s) 218, 476, 600
MmeI TCCRAC 2 cut(s) 585, 672
MnlI CCTC 9 cut(s) 47, 76, 272, 300, 368, 557, 585, 667, 910
Mph1103I ATGCAT 2 cut(s) 6, 10
MseI TTAA 2 cut(s) 456, 928
MspI CCGG 2 cut(s) 186, 477
MspR9I CCNGG 3 cut(s) 255, 523, 875
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 3 cut(s) 255, 523, 875
MvnI CGCG 2 cut(s) 267, 737
MwoI GCNNNNNNNGC 1 cut(s) 337
NcoI CCATGG 1 cut(s) 585
NdeII GATC 6 cut(s) 147, 276, 297, 486, 570, 717
NlaIV GGNNCC 5 cut(s) 58, 76, 202, 259, 379
NmuCI GTSAC 3 cut(s) 116, 214, 353
NsiI ATGCAT 2 cut(s) 6, 10
NspI RCATGY 3 cut(s) 8, 12, 108
PaeI GCATGC 3 cut(s) 8, 12, 108
PagI TCATGA 1 cut(s) 621
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 156, 570
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 4 cut(s) 322, 502, 763, 792
PflMI CCANNNNNTGG 1 cut(s) 567
PkrI GCNGC 1 cut(s) 191
PleI GAGTC 3 cut(s) 218, 475, 600
PmaCI CACGTG 1 cut(s) 802
PmlI CACGTG 1 cut(s) 802
PpsI GAGTC 3 cut(s) 218, 475, 600
Ppu21I YACGTR 1 cut(s) 802
PpuMI RGGWCCY 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 253, 521, 873
PspCI CACGTG 1 cut(s) 802
PspGI CCWGG 3 cut(s) 253, 521, 873
PspN4I GGNNCC 5 cut(s) 58, 76, 202, 259, 379
PspOMI GGGCCC 1 cut(s) 200
PspPI GGNCC 6 cut(s) 74, 129, 200, 201, 287, 519
PspPPI RGGWCCY 1 cut(s) 287
PsrI GAACNNNNNNTAC 2 cut(s) 800, 832
PstNI CAGNNNCTG 1 cut(s) 495
RsaI GTAC 1 cut(s) 887
RsaNI GTAC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 456, 928
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 6 cut(s) 147, 276, 297, 486, 570, 717
Sau96I GGNCC 6 cut(s) 74, 129, 200, 201, 287, 519
SchI GAGTC 3 cut(s) 218, 476, 600
ScrFI CCNGG 3 cut(s) 255, 523, 875
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 2 cut(s) 549, 770
SinI GGWCC 3 cut(s) 74, 287, 519
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
SphI GCATGC 3 cut(s) 8, 12, 108
Sse9I AATT 1 cut(s) 47
SsiI CCGC 3 cut(s) 112, 189, 265
StyD4I CCNGG 3 cut(s) 253, 521, 873
StyI CCWWGG 2 cut(s) 60, 585
TaaI ACNGT 2 cut(s) 74, 733
TaiI ACGT 3 cut(s) 456, 567, 804
TaqI TCGA 4 cut(s) 99, 153, 366, 573
TasI AATT 1 cut(s) 47
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 4 cut(s) 322, 502, 763, 792
Tru1I TTAA 2 cut(s) 456, 928
Tru9I TTAA 2 cut(s) 456, 928
TscAI CASTG 4 cut(s) 223, 279, 637, 667
TseFI GTSAC 3 cut(s) 116, 214, 353
Tsp45I GTSAC 3 cut(s) 116, 214, 353
TspDTI ATGAA 1 cut(s) 869
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 4 cut(s) 223, 279, 637, 667
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 3 cut(s) 74, 287, 519
XagI CCTNNNNNAGG 1 cut(s) 252
XceI RCATGY 3 cut(s) 8, 12, 108
Zsp2I ATGCAT 2 cut(s) 6, 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.