MD13G1088900.v1.1
MYB Family

bHLH-MYC and R2R3-MYB transcription factors N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012163
Physical Location & Seq
Forward (+)
6230153 .. 6232599
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1088900.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGAAGAACATCTGAACCCCTTAGCTGTGACTCATCTGCTTCAACACACCCTGAGAAGCTTGTGCAATCACGAAAACTCTCAGTGGATTTATGCAGTCTTCTGGAGGATCCTTCCTAGAAACTATCCACCACCCAAATGGGAAGGTCATGGAGCTTATGATAGGTCAAGAGGAAACAGAAGGAACTGGATATTGGTTTGGGAAGATGGATTCTGCAACTTTGGTGCGTCCTCACCGGCTCATGATCAGATCAACTCCAGTTCTGATCAGTGCCCTGCGGGACCTTCATCAATGTATAGCAACTTAAGCGATCAATTTCAACTATACAATGGCCTGCAACCTGAGCTTTTCTTCAAGATGTCCCATGAGATCTACAATTATGGAGAAGGGTTGATCGGGAAAGTGGCGGCAGATCATAGTCATAAGTGGATACACAAAGAACCAAATGATCAAGAAATCAACTTCTTATCAGCATGGCATAACTCAGCAGACATGCACCCTAGAACATGGGAAGCCCAATTCCAGTCTGGCATAAAGACCATAGCCCTGATAGCAGTTAGAGAAGGTGTCGTTCAGTTAGGAGCTGTTCACAAGGTGATTGAAGACTTGAGCTATGTAGTTCTGCTAAGAAAGAAGTTCAGCTACATCGAAAGCATCCCCGGAGTGCTTCTCCCACACCCATCATCTCCATCCTTCCCCTTCAAGCCTGATCGTCATCATGGCTATGCATACGGTCCCACCCCAGATTATCATCATCATCCTCAACATGCATGGCATTTCCAAGGTGGCACAAACCCTCTTGCACCACCTCCAACTCATGACTTCATGTATGATCATCCCCAATATCTCAACCCTAATCAAGTAGTTCCACTGAAAAACATAACCCCCTCCATGAGCAGCCTTGAAGCCCTCCTCTCTAAGCTTCCCTCCGTCGTGCCGACGAATGACGATCATCATCATCATCATCCACAGCCGCACGATCAATCCCAGTTTGTGTTATCTCATGGACCTGTGGACTTCATGGCAATGACCGAGCAGAAAGTGGCCAAGGAGGAGACTACTGATCATGATGACGGTCATGACCGTAACGAAGAATATAGGCCGTCGGATTACCAAAACGTCGGTGAAACCAGCAATTCTATTTCTGCTTATTCCCGTCAACAGCAACAGCATTTCCATCACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bHLH-MYC_N

Protein Analysis

396

Amino Acids

45.34

Weight (kDa)

6.08

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 14 - 213 1e-33 bHLH-MYC and R2R3-MYB transcription factors N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60060 AT1G60060
fragaria_vesca FvH4_4g34860
malus_domestica MD13G1088900.v1.1 MD16G1089000.v1.1
prunus_persica Prupe.1G256900_v2.0.a1
pyrus_communis pycom13g07700 pycom16g07620
rosa_chinensis RchiOBHm_Chr4g0444251
rosa_laevigata RLG00000005846
rosa_multiflora Rmu_sc0002841.1_g000017
rosa_roxburghii Rroxscaffold_5G00384820
rosa_rugosa Rorug04G0350900 Rorug04G0351000
rosa_samantha Rh4AG411900 Rh4BG423200 Rh4CG437400 Rh4DG418400
rosa_wichuraiana Rw4G035360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 278, 407, 974
AclWI GGATC 2 cut(s) 103, 116
AcoI YGGCCR 1 cut(s) 1044
AdeI CACNNNGTG 1 cut(s) 595
AflII CTTAAG 1 cut(s) 304
AgsI TTSAA 6 cut(s) 44, 320, 355, 602, 703, 905
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 8 cut(s) 26, 60, 155, 346, 584, 612, 642, 922
AluI AGCT 8 cut(s) 26, 60, 155, 346, 584, 612, 642, 922
Alw26I GTCTC 1 cut(s) 1049
AlwI GGATC 2 cut(s) 103, 116
AoxI GGCC 3 cut(s) 331, 1044, 1100
ApeKI GCWGC 1 cut(s) 897
AspS9I GGNCC 3 cut(s) 281, 734, 1007
AsuC2I CCSGG 1 cut(s) 660
AsuHPI GGTGA 3 cut(s) 225, 607, 1136
AvaII GGWCC 3 cut(s) 281, 734, 1007
BaeGI GKGCMC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 1046
BamHI GGATCC 1 cut(s) 108
BarI GAAGNNNNNNTAC 2 cut(s) 626, 658
BbsI GAAGAC 2 cut(s) 91, 609
BbvI GCAGC 1 cut(s) 909
BccI CCATC 4 cut(s) 200, 688, 697, 1185
BceAI ACGGC 1 cut(s) 1087
BciVI GTATCC 1 cut(s) 423
BclI TGATCA 5 cut(s) 244, 265, 448, 832, 1063
BcnI CCSGG 1 cut(s) 660
BcoDI GTCTC 1 cut(s) 1049
BfaI CTAG 2 cut(s) 117, 501
BfrI CTTAAG 1 cut(s) 304
BfuI GTATCC 1 cut(s) 423
BglII AGATCT 1 cut(s) 369
BisI GCNGC 3 cut(s) 408, 898, 974
BlsI GCNGC 3 cut(s) 409, 899, 975
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 3 cut(s) 281, 734, 1007
BmgT120I GGNCC 3 cut(s) 281, 734, 1007
BmiI GGNNCC 3 cut(s) 110, 282, 736
BmrFI CCNGG 1 cut(s) 660
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 1 cut(s) 663
BmuI ACTGGG 1 cut(s) 982
BpiI GAAGAC 2 cut(s) 91, 609
BplI GAGNNNNNCTC 2 cut(s) 654, 686
BpmI CTGGAG 2 cut(s) 124, 241
Bpu10I CCTNAGC 2 cut(s) 22, 342
BpuEI CTTGAG 1 cut(s) 628
BpuMI CCSGG 1 cut(s) 660
BsaBI GATNNNNATC 3 cut(s) 714, 750, 954
BsaJI CCNNGG 3 cut(s) 658, 781, 1047
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 4 cut(s) 191, 258, 523, 988
Bse3DI GCAATG 1 cut(s) 1032
Bse8I GATNNNNATC 3 cut(s) 714, 750, 954
BseDI CCNNGG 3 cut(s) 658, 781, 1047
BseGI GGATG 5 cut(s) 654, 689, 757, 835, 964
BseJI GATNNNNATC 3 cut(s) 714, 750, 954
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 4 cut(s) 44, 95, 333, 498
BseNI ACTGG 4 cut(s) 191, 258, 523, 988
BseRI GAGGAG 2 cut(s) 902, 1067
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 1 cut(s) 909
BshFI GGCC 3 cut(s) 333, 1046, 1102
BsiSI CCGG 2 cut(s) 236, 660
BslFI GGGAC 3 cut(s) 294, 346, 720
BsmAI GTCTC 1 cut(s) 1049
BsmFI GGGAC 3 cut(s) 294, 346, 720
BsnI GGCC 3 cut(s) 333, 1046, 1102
Bsp1286I GDGCHC 1 cut(s) 275
BspACI CCGC 3 cut(s) 278, 407, 974
BspANI GGCC 3 cut(s) 333, 1046, 1102
BspCNI CTCAG 4 cut(s) 45, 94, 334, 497
BspHI TCATGA 4 cut(s) 241, 817, 1066, 1078
BspLI GGNNCC 3 cut(s) 110, 282, 736
BspPI GGATC 2 cut(s) 103, 116
BspTI CTTAAG 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 1032
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 4 cut(s) 191, 258, 523, 988
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 658, 781, 1047
BssT1I CCWWGG 2 cut(s) 781, 1047
Bst4CI ACNGT 3 cut(s) 734, 1076, 1085
BstAFI CTTAAG 1 cut(s) 304
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 7 cut(s) 22, 53, 81, 342, 484, 626, 918
BstF5I GGATG 5 cut(s) 654, 689, 757, 835, 964
BstMAI GTCTC 1 cut(s) 1049
BstMWI GCNNNNNNNGC 3 cut(s) 306, 343, 551
BstNSI RCATGY 2 cut(s) 496, 770
BstSCI CCNGG 1 cut(s) 658
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 909
BstV2I GAAGAC 2 cut(s) 91, 609
BstX2I RGATCY 2 cut(s) 108, 369
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 108, 369
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 3 cut(s) 333, 1046, 1102
BtsCI GGATG 5 cut(s) 654, 689, 757, 835, 964
BtsIMutI CAGTG 3 cut(s) 89, 275, 869
Cac8I GCNNGC 1 cut(s) 335
CciI TCATGA 4 cut(s) 241, 817, 1066, 1078
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 3 cut(s) 281, 734, 1007
CseI GACGC 1 cut(s) 216
DdeI CTNAG 7 cut(s) 22, 53, 81, 342, 484, 626, 918
DraIII CACNNNGTG 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 1044
Eco130I CCWWGG 2 cut(s) 781, 1047
Eco47I GGWCC 3 cut(s) 281, 734, 1007
EcoO109I RGGNCCY 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 781, 1047
EcoT22I ATGCAT 2 cut(s) 730, 772
ErhI CCWWGG 2 cut(s) 781, 1047
FaqI GGGAC 3 cut(s) 294, 346, 720
FauI CCCGC 1 cut(s) 271
FbaI TGATCA 5 cut(s) 244, 265, 448, 832, 1063
Fnu4HI GCNGC 3 cut(s) 408, 898, 974
FokI GGATG 5 cut(s) 641, 676, 744, 822, 951
Fsp4HI GCNGC 3 cut(s) 408, 898, 974
FspBI CTAG 2 cut(s) 117, 501
GluI GCNGC 3 cut(s) 408, 898, 974
GsuI CTGGAG 2 cut(s) 124, 241
HaeIII GGCC 3 cut(s) 333, 1046, 1102
HapII CCGG 2 cut(s) 236, 660
HgaI GACGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 1160
HindII GTYRAC 1 cut(s) 1160
HindIII AAGCTT 2 cut(s) 58, 920
HinfI GANTC 2 cut(s) 31, 210
HpaII CCGG 2 cut(s) 236, 660
HphI GGTGA 3 cut(s) 225, 607, 1136
Hpy166II GTNNAC 3 cut(s) 589, 1015, 1160
Hpy188I TCNGA 4 cut(s) 15, 249, 265, 1108
Hpy8I GTNNAC 3 cut(s) 589, 1015, 1160
Hpy99I CGWCG 4 cut(s) 935, 943, 1108, 1124
HpyAV CCTTC 8 cut(s) 122, 137, 174, 294, 380, 557, 703, 709
HpyCH4III ACNGT 3 cut(s) 734, 1076, 1085
HpyCH4IV ACGT 1 cut(s) 1119
HpyCH4V TGCA 8 cut(s) 66, 95, 216, 337, 496, 728, 770, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 306, 343, 551
HpyF3I CTNAG 7 cut(s) 22, 53, 81, 342, 484, 626, 918
HpySE526I ACGT 1 cut(s) 1119
Ksp22I TGATCA 5 cut(s) 244, 265, 448, 832, 1063
LmnI GCTCC 2 cut(s) 152, 581
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 1 cut(s) 663
MaeI CTAG 2 cut(s) 117, 501
MaeII ACGT 1 cut(s) 1119
MaeIII GTNAC 2 cut(s) 28, 1085
MboII GAAGA 6 cut(s) 17, 91, 215, 343, 614, 1103
MflI RGATCY 2 cut(s) 108, 369
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 4 cut(s) 314, 376, 518, 1135
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 2 cut(s) 836, 1086
Mox20I TGGCCA 1 cut(s) 1046
Mph1103I ATGCAT 2 cut(s) 730, 772
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 3 cut(s) 136, 723, 1025
Msp20I TGGCCA 1 cut(s) 1046
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 2 cut(s) 236, 660
MspR9I CCNGG 1 cut(s) 660
MwoI GCNNNNNNNGC 3 cut(s) 306, 343, 551
NciI CCSGG 1 cut(s) 660
NlaIV GGNNCC 3 cut(s) 110, 282, 736
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 2 cut(s) 730, 772
NspI RCATGY 2 cut(s) 496, 770
PagI TCATGA 4 cut(s) 241, 817, 1066, 1078
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 3 cut(s) 409, 899, 975
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
PspN4I GGNNCC 3 cut(s) 110, 282, 736
PspPI GGNCC 3 cut(s) 281, 734, 1007
PspPPI RGGWCCY 1 cut(s) 281
PsuI RGATCY 2 cut(s) 108, 369
RseI CAYNNNNRTG 3 cut(s) 136, 723, 1025
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 3 cut(s) 408, 898, 974
Sau96I GGNCC 3 cut(s) 281, 734, 1007
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 1 cut(s) 663
SinI GGWCC 3 cut(s) 281, 734, 1007
SmiMI CAYNNNNRTG 3 cut(s) 136, 723, 1025
SmlI CTYRAG 2 cut(s) 304, 607
SmoI CTYRAG 2 cut(s) 304, 607
Sse9I AATT 4 cut(s) 314, 376, 518, 1135
SsiI CCGC 3 cut(s) 278, 407, 974
SspMI CTAG 2 cut(s) 117, 501
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 2 cut(s) 781, 1047
TaaI ACNGT 3 cut(s) 734, 1076, 1085
TaiI ACGT 1 cut(s) 1122
TaqI TCGA 1 cut(s) 648
TaqII GACCGA 1 cut(s) 1046
TasI AATT 4 cut(s) 314, 376, 518, 1135
TauI GCSGC 2 cut(s) 410, 976
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 3 cut(s) 89, 275, 876
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 897
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 3 cut(s) 276, 814, 1009
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 3 cut(s) 89, 275, 876
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 3 cut(s) 281, 734, 1007
XceI RCATGY 2 cut(s) 496, 770
XcmI CCANNNNNNNNNTGG 2 cut(s) 135, 524
XspI CTAG 2 cut(s) 117, 501
Zsp2I ATGCAT 2 cut(s) 730, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.