MD16G1089000.v1.1
MYB Family

bHLH-MYC and R2R3-MYB transcription factors N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012163
Physical Location & Seq
Forward (+)
6151026 .. 6153843
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1089000.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGGAAGAACATCTGAACCCCTTAGCTGTGACTCATCTGCTTCAACACACCCTGAGAAGCTTGTGCATTCATGAAAACTCTCAGTGGATTTATGCAGTCTTCTGGAGGATCCTTCCTAGAAACTATCCACCACCCAAATGGGAAGGTCATGGAGCTTATGATAGGTCAAGAGGAAACAGAAGGAACTGGATATTGGTTTGGGAAGATGGATTCTGCAACTTTGGTGCCTCCTCAGCAGCTCATGAGATCAACTCCAGTTCTGATCAGTGCCCTGCAGGACCTTCATCAATGTATGGCAACTCATGTGATCAATTTCAACAAAACAATGGCTTGCAACCTGAGCTTTTCTTCAAGATGTCCCATGAGATCTACAACTACGGAGAAGGGTTGATAGGGAAAGTGGCGGTAGATCATAGTCATAAGTGGATACACAACGAACCAAATGATCAAGAAATCAACTTCTTATCAACATGGCACAACTCAGCAGACACGCACCCTAGAACATGGGAAGCCCAGTTCCAGTCTGGCATAAAGACCATAGCCCTGATAGCAGTTAGAGAAGGTGTTATTCAGTTAGGAGCTGTTCACAAGGTGATTGAAGACTTGAGCTATGTAGTTCTACTAAGAAAGAAGTTCGGCTACATCGAAAGCATCCCCGGAGTGCTTCTCCCACACCCATCATCTCCACCCTTCCCCTTCAAGCCTGATCCTCATCATCATCATGGCTATACATATGGTCTCACCCCAGATCACCATCATCATGCATGGCATCTCCAAGCAGGGACAAACCCTCTTGCACCACCTCCAACTCATGACTTCATGTATGATCATCACCAATATCTCAACCCTAATCAAGTGGTTCCTTTGAAAAACATAACCCCCTCCATGAGCAGCCTTGAAGCCCTCCTCTCTAAGCTTCCGTCGGTCGTGCCGACGAATCACGATCATCATCCTCAGCCGCATGATCAATCCCAGTTTTTATTATCTCATGGTCATGGACCTGTGGATTTTATGGCAATGACGGACCAGAAAGTGGCCAAGGAGGAAACTAGTGACAATGATGATCATGTTCGTAACGAAGAATATAGGCCGTCCGATGATCAAAACAACGTCGGTGAAACCAGCACTTCTGTGTCTGCTTATGCCCATCAACAGCAACAGTATTTTCATCACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bHLH-MYC_N

Protein Analysis

393

Amino Acids

44.77

Weight (kDa)

6.08

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 14 - 212 3.8e-33 bHLH-MYC and R2R3-MYB transcription factors N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60060 AT1G60060
fragaria_vesca FvH4_4g34860
malus_domestica MD13G1088900.v1.1 MD16G1089000.v1.1
prunus_persica Prupe.1G256900_v2.0.a1
pyrus_communis pycom13g07700 pycom16g07620
rosa_chinensis RchiOBHm_Chr4g0444251
rosa_laevigata RLG00000005846
rosa_multiflora Rmu_sc0002841.1_g000017
rosa_roxburghii Rroxscaffold_5G00384820
rosa_rugosa Rorug04G0350900 Rorug04G0351000
rosa_samantha Rh4AG411900 Rh4BG423200 Rh4CG437400 Rh4DG418400
rosa_wichuraiana Rw4G035360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 1033
AciI CCGC 2 cut(s) 404, 959
AclWI GGATC 3 cut(s) 103, 116, 701
AcoI YGGCCR 1 cut(s) 1035
AdeI CACNNNGTG 1 cut(s) 592
AfiI CCNNNNNNNGG 1 cut(s) 1033
AgsI TTSAA 7 cut(s) 44, 317, 352, 599, 700, 868, 899
AhlI ACTAGT 1 cut(s) 1049
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 1 cut(s) 1130
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 8 cut(s) 26, 60, 155, 239, 343, 581, 609, 916
AluI AGCT 8 cut(s) 26, 60, 155, 239, 343, 581, 609, 916
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 3 cut(s) 103, 116, 701
AoxI GGCC 2 cut(s) 1035, 1088
ApeKI GCWGC 2 cut(s) 236, 891
AspS9I GGNCC 3 cut(s) 278, 998, 1024
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 6 cut(s) 604, 733, 743, 824, 1127, 1163
AvaII GGWCC 3 cut(s) 278, 998, 1024
BaeGI GKGCMC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 1037
BamHI GGATCC 1 cut(s) 108
BanI GGYRCC 1 cut(s) 224
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BbsI GAAGAC 2 cut(s) 91, 606
BbvCI CCTCAGC 2 cut(s) 232, 954
BbvI GCAGC 2 cut(s) 248, 903
BccI CCATC 4 cut(s) 200, 685, 762, 1155
BceAI ACGGC 1 cut(s) 1075
BciVI GTATCC 1 cut(s) 420
BclI TGATCA 7 cut(s) 262, 307, 445, 826, 964, 1063, 1099
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 1 cut(s) 743
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 3 cut(s) 117, 498, 1050
BfmI CTRYAG 1 cut(s) 273
BfuI GTATCC 1 cut(s) 420
BglII AGATCT 1 cut(s) 366
BisI GCNGC 3 cut(s) 237, 892, 959
BlsI GCNGC 3 cut(s) 238, 893, 960
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 3 cut(s) 278, 998, 1024
BmgT120I GGNCC 3 cut(s) 278, 998, 1024
BmiI GGNNCC 3 cut(s) 110, 226, 861
BmrFI CCNGG 1 cut(s) 657
BmrI ACTGGG 2 cut(s) 508, 967
BmsI GCATC 2 cut(s) 660, 778
BmuI ACTGGG 2 cut(s) 508, 967
BpiI GAAGAC 2 cut(s) 91, 606
BplI GAGNNNNNCTC 4 cut(s) 236, 268, 651, 683
BpmI CTGGAG 2 cut(s) 124, 238
Bpu10I CCTNAGC 4 cut(s) 22, 232, 339, 954
BpuEI CTTGAG 1 cut(s) 625
BpuMI CCSGG 1 cut(s) 657
BsaBI GATNNNNATC 3 cut(s) 711, 753, 948
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 655, 1038
Bsc4I CCNNNNNNNGG 1 cut(s) 1033
Bse1I ACTGG 5 cut(s) 191, 255, 514, 520, 973
Bse3DI GCAATG 1 cut(s) 1023
Bse8I GATNNNNATC 3 cut(s) 711, 753, 948
BseDI CCNNGG 2 cut(s) 655, 1038
BseGI GGATG 2 cut(s) 651, 949
BseJI GATNNNNATC 3 cut(s) 711, 753, 948
BseLI CCNNNNNNNGG 1 cut(s) 1033
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 6 cut(s) 44, 95, 246, 330, 495, 968
BseNI ACTGG 5 cut(s) 191, 255, 514, 520, 973
BseRI GAGGAG 2 cut(s) 220, 896
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 2 cut(s) 248, 903
Bsh1285I CGRYCG 1 cut(s) 927
BshFI GGCC 2 cut(s) 1037, 1090
BshNI GGYRCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 927
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 2 cut(s) 343, 796
BslI CCNNNNNNNGG 1 cut(s) 1033
BsmAI GTCTC 1 cut(s) 743
BsmFI GGGAC 2 cut(s) 343, 796
BsmI GAATGC 1 cut(s) 66
BsnI GGCC 2 cut(s) 1037, 1090
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 1 cut(s) 272
BspACI CCGC 2 cut(s) 404, 959
BspANI GGCC 2 cut(s) 1037, 1090
BspCNI CTCAG 6 cut(s) 45, 94, 245, 331, 494, 967
BspHI TCATGA 3 cut(s) 70, 241, 811
BspLI GGNNCC 3 cut(s) 110, 226, 861
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 3 cut(s) 103, 116, 701
BspT107I GGYRCC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 1023
BsrI ACTGG 5 cut(s) 191, 255, 514, 520, 973
BssECI CCNNGG 2 cut(s) 655, 1038
BssT1I CCWWGG 1 cut(s) 1038
Bst4CI ACNGT 1 cut(s) 1161
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 9 cut(s) 22, 53, 81, 232, 339, 481, 623, 912, 954
BstF5I GGATG 2 cut(s) 651, 949
BstMAI GTCTC 1 cut(s) 743
BstMCI CGRYCG 1 cut(s) 927
BstMWI GCNNNNNNNGC 3 cut(s) 233, 340, 548
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 248, 903
BstV2I GAAGAC 2 cut(s) 91, 606
BstX2I RGATCY 2 cut(s) 108, 366
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 108, 366
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 1037, 1090
BtsCI GGATG 2 cut(s) 651, 949
BtsIMutI CAGTG 2 cut(s) 89, 272
Cac8I GCNNGC 1 cut(s) 332
CciI TCATGA 3 cut(s) 70, 241, 811
Cfr13I GGNCC 3 cut(s) 278, 998, 1024
DdeI CTNAG 9 cut(s) 22, 53, 81, 232, 339, 481, 623, 912, 954
DraIII CACNNNGTG 1 cut(s) 592
EaeI YGGCCR 1 cut(s) 1035
Eco130I CCWWGG 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 743
Eco47I GGWCC 3 cut(s) 278, 998, 1024
EcoO109I RGGNCCY 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 766
ErhI CCWWGG 1 cut(s) 1038
FaqI GGGAC 2 cut(s) 343, 796
FauNDI CATATG 1 cut(s) 733
FbaI TGATCA 7 cut(s) 262, 307, 445, 826, 964, 1063, 1099
Fnu4HI GCNGC 3 cut(s) 237, 892, 959
FokI GGATG 2 cut(s) 638, 936
Fsp4HI GCNGC 3 cut(s) 237, 892, 959
FspBI CTAG 3 cut(s) 117, 498, 1050
GluI GCNGC 3 cut(s) 237, 892, 959
GsuI CTGGAG 2 cut(s) 124, 238
HaeIII GGCC 2 cut(s) 1037, 1090
HapII CCGG 1 cut(s) 657
HindIII AAGCTT 2 cut(s) 58, 914
HinfI GANTC 3 cut(s) 31, 210, 937
HpaII CCGG 1 cut(s) 657
HphI GGTGA 6 cut(s) 604, 733, 743, 824, 1127, 1163
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 3 cut(s) 15, 262, 1096
Hpy188III TCNNGA 8 cut(s) 71, 103, 168, 242, 352, 449, 812, 941
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 3 cut(s) 925, 937, 1115
HpyAV CCTTC 8 cut(s) 122, 137, 174, 291, 377, 554, 700, 706
HpyCH4III ACNGT 1 cut(s) 1161
HpyCH4IV ACGT 1 cut(s) 1110
HpyCH4V TGCA 7 cut(s) 66, 95, 216, 275, 334, 764, 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 340, 548
HpyF3I CTNAG 9 cut(s) 22, 53, 81, 232, 339, 481, 623, 912, 954
HpySE526I ACGT 1 cut(s) 1110
Ksp22I TGATCA 7 cut(s) 262, 307, 445, 826, 964, 1063, 1099
LmnI GCTCC 2 cut(s) 152, 578
Lsp1109I GCAGC 2 cut(s) 248, 903
LweI GCATC 2 cut(s) 660, 778
MaeI CTAG 3 cut(s) 117, 498, 1050
MaeII ACGT 1 cut(s) 1110
MaeIII GTNAC 3 cut(s) 28, 1052, 1073
MboII GAAGA 6 cut(s) 17, 91, 215, 340, 611, 1091
MflI RGATCY 2 cut(s) 108, 366
MhlI GDGCHC 1 cut(s) 272
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 830
Mox20I TGGCCA 1 cut(s) 1037
Mph1103I ATGCAT 1 cut(s) 766
MscI TGGCCA 1 cut(s) 1037
MslI CAYNNNNRTG 5 cut(s) 136, 720, 759, 993, 1130
Msp20I TGGCCA 1 cut(s) 1037
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 657
Mva1269I GAATGC 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 233, 340, 548
NciI CCSGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 733
NlaIV GGNNCC 3 cut(s) 110, 226, 861
NmuCI GTSAC 2 cut(s) 28, 1052
NsiI ATGCAT 1 cut(s) 766
OliI CACNNNNGTG 1 cut(s) 1130
PagI TCATGA 3 cut(s) 70, 241, 811
PcsI WCGNNNNNNNCGW 2 cut(s) 642, 929
PctI GAATGC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 210, 937
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 3 cut(s) 238, 893, 960
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 278
PspN4I GGNNCC 3 cut(s) 110, 226, 861
PspPI GGNCC 3 cut(s) 278, 998, 1024
PspPPI RGGWCCY 1 cut(s) 278
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 2 cut(s) 108, 366
RseI CAYNNNNRTG 5 cut(s) 136, 720, 759, 993, 1130
SatI GCNGC 3 cut(s) 237, 892, 959
Sau96I GGNCC 3 cut(s) 278, 998, 1024
SbfI CCTGCAGG 1 cut(s) 277
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 1 cut(s) 657
SdaI CCTGCAGG 1 cut(s) 277
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 2 cut(s) 660, 778
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 3 cut(s) 278, 998, 1024
SmiMI CAYNNNNRTG 5 cut(s) 136, 720, 759, 993, 1130
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
SpeI ACTAGT 1 cut(s) 1049
Sse8387I CCTGCAGG 1 cut(s) 277
Sse9I AATT 1 cut(s) 311
SsiI CCGC 2 cut(s) 404, 959
SspMI CTAG 3 cut(s) 117, 498, 1050
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 1161
TaiI ACGT 1 cut(s) 1113
TaqI TCGA 1 cut(s) 645
TaqII GACCGA 1 cut(s) 913
TasI AATT 1 cut(s) 311
TauI GCSGC 1 cut(s) 961
TfiI GAWTC 2 cut(s) 210, 937
TscAI CASTG 2 cut(s) 89, 272
TseFI GTSAC 2 cut(s) 28, 1052
TseI GCWGC 2 cut(s) 236, 891
Tsp45I GTSAC 2 cut(s) 28, 1052
TspDTI ATGAA 5 cut(s) 59, 87, 273, 808, 1157
TspGWI ACGGA 3 cut(s) 393, 909, 1037
TspRI CASTG 2 cut(s) 89, 272
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 3 cut(s) 278, 998, 1024
XcmI CCANNNNNNNNNTGG 2 cut(s) 135, 521
XspI CTAG 3 cut(s) 117, 498, 1050
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.