Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003182

Physical Location & Seq

Genomic Coordinates Reverse (-)

8138958 .. 8142074

Gene Structure 3117 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1112000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGGCTTCCATCGCTGCATCAAACGTCGTCGCTCTAAAGTCCGCCGGTAAATTTGGAGCTCCCGCCGACGCCAGGGCCTGCCAGTTTAAGCAATGGGCCCCGATTGGCGGCGCAGCCGGATCGGGTCGGAGCATTGGGCTTCAGTACAGATCCAAGCGCTCCTCCACTTCCTCTGGTCACGTGAGGGCTCAGGTGGCGACGCTTGAGCAAGCGAGCGCCGGGGAAGCCAAAATCGTGGAGGGTCCGGTGGTGGTGGTGACCGGAGCTTCTAGAGGCATTGGCAGAGCAATTGCTCTGTCTTTGGGCAAAGCTGGTTGCAGGGTCCTCGTTAATTACGCAAGGTCATCGAAGGAGGCCGAGGAGGTTTCCAAAGAGATTGAGGCGTTCGGTGGACAAGCTATTACTTTCGGAGGAGATGTTTCGAAAGAAGAAGATGTTGAGGCGATGCTCAAAACAGCGCTTGATGCTTGGGGAACCGTTGATGTACTGATAAACAATGCAGGAATTACCAGGGATGGTCTATTGATGAGAATGAAGTTAAAACAATGGCAGGAGGTCATTGATCTAAATCTTACTGGTGTATATCTATGCACCCAGGCAGCAGCTAAAATCATGATGAAGAAGAAGAAGGGAAGGATTATCAATATAGCATCCGTTGTTGGTCTAGTTGGAAATGTTGGACAAGCCAACTACAGTGCTGCAAAAGCAGGAGTAATTGGTCTGACCAAGACTGTTGCTAAGGAGTACTCCAGCAGACACATCAATGTTAATGCTGTTGCCCCGGGCTTTATTGCATCAGACATGACTGCCAAGCTAGGCGATGACATTGAGAAGAAAATTTTGGAGACTATCCCATTAGGAAGATACGGCCAACCCGACGAAGTTGCTGGACTGGTGGAATTCTTGGCCCTTAGTCCAGCGGCCAGTTACATGACCGGACAGGTGCTCACCATTGATGGAGGGATGGTGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004312 GO:0004316 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016746 GO:0016747 GO:0031967 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212

Pfam Domains

Protein Families

adh_short adh_short_C2

Protein Analysis

325

Amino Acids

33.73

Weight (kDa)

9.3

Isoelectric Point (pI)

32.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	83 - 275	3.2e-65	short chain dehydrogenase
KR	PF08659	84 - 238	4e-21	KR domain
adh_short_C2	PF13561	88 - 322	5.2e-65	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24360
fragaria_vesca	FvH4_4g24340 FvH4_5g07680 FvH4_5g07680
malus_domestica	MD13G1112000.v1.1 MD14G1162300.v1.1 MD16G1111600.v1.1
prunus_persica	Prupe.1G233400_v2.0.a1 Prupe.3G006500_v2.0.a1 Prupe.3G006500_v2.0.a1 Prupe.5G154900_v2.0.a1 Prupe.5G154900_v2.0.a1 Prupe.5G154900_v2.0.a1
pyrus_communis	pycom13g09740 pycom14g13520 pycom16g09460
rosa_chinensis	RchiOBHm_Chr4g0431201 RchiOBHm_Chr7g0190791
rosa_laevigata	RLG00000004498 RLG00000006920
rosa_multiflora	Rmu_sc0005258.1_g000006 Rmu_sc0032787.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264310 Rroxscaffold_5G00372740
rosa_rugosa	Rorug04G0249100 Rorug06G0506900
rosa_samantha	Rh4AG305200 Rh4BG312500 Rh4CG328800 Rh4DG309100 Rh7AG113600 Rh7BG116100 Rh7CG118700 Rh7CG119000 Rh7DG117600
rosa_wichuraiana	Rw4G026520 Rw7G009920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	42, 63, 108, 920
AclWI	GGATC	2 cut(s)	127, 144
AcoI	YGGCCR	2 cut(s)	868, 921
AcsI	RAATTY	3 cut(s)	50, 837, 899
AcuI	CTGAAG	1 cut(s)	125
AcvI	CACGTG	1 cut(s)	181
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	3 cut(s)	146, 486, 746
AfeI	AGCGCT	2 cut(s)	158, 459
AfiI	CCNNNNNNNGG	2 cut(s)	72, 107
AjnI	CCWGG	3 cut(s)	71, 509, 594
AjuI	GAANNNNNNNTTGG	2 cut(s)	824, 856
AluBI	AGCT	6 cut(s)	59, 266, 311, 398, 605, 814
AluI	AGCT	6 cut(s)	59, 266, 311, 398, 605, 814
Alw21I	GWGCWC	2 cut(s)	61, 948
Alw26I	GTCTC	1 cut(s)	839
AlwI	GGATC	2 cut(s)	127, 144
AlwNI	CAGNNNCTG	1 cut(s)	78
Ama87I	CYCGRG	1 cut(s)	781
Aor51HI	AGCGCT	2 cut(s)	158, 459
AoxI	GGCC	6 cut(s)	75, 96, 354, 868, 906, 921
ApaI	GGGCCC	1 cut(s)	100
ApeKI	GCWGC	5 cut(s)	14, 113, 599, 602, 698
ApoI	RAATTY	3 cut(s)	50, 837, 899
AspLEI	GCGC	4 cut(s)	113, 159, 218, 460
AspS9I	GGNCC	6 cut(s)	75, 96, 97, 242, 322, 907
AsuC2I	CCSGG	3 cut(s)	220, 782, 783
AsuHPI	GGTGA	2 cut(s)	268, 940
AsuII	TTCGAA	1 cut(s)	422
AvaI	CYCGRG	1 cut(s)	781
AvaII	GGWCC	2 cut(s)	242, 322
BaeGI	GKGCMC	1 cut(s)	100
BanII	GRGCYC	3 cut(s)	61, 100, 190
BbrPI	CACGTG	1 cut(s)	181
Bbv12I	GWGCWC	2 cut(s)	61, 948
BbvI	GCAGC	4 cut(s)	125, 611, 614, 685
BccI	CCATC	4 cut(s)	17, 509, 950, 958
BceAI	ACGGC	1 cut(s)	883
BcgI	CGANNNNNNTGC	8 cut(s)	102, 136, 307, 327, 341, 361, 866, 900
BciT130I	CCWGG	3 cut(s)	73, 511, 596
BcnI	CCSGG	3 cut(s)	220, 782, 783
BcoDI	GTCTC	1 cut(s)	839
BfaI	CTAG	3 cut(s)	270, 665, 815
BfmI	CTRYAG	1 cut(s)	691
BfoI	RGCGCY	3 cut(s)	160, 219, 461
BisI	GCNGC	7 cut(s)	15, 109, 114, 600, 603, 699, 921
BlsI	GCNGC	7 cut(s)	16, 110, 115, 601, 604, 700, 922
BmcAI	AGTACT	1 cut(s)	746
Bme1390I	CCNGG	6 cut(s)	73, 220, 511, 596, 782, 783
Bme18I	GGWCC	2 cut(s)	242, 322
BmeT110I	CYCGRG	1 cut(s)	781
BmgT120I	GGNCC	6 cut(s)	75, 96, 97, 242, 322, 907
BmiI	GGNNCC	5 cut(s)	98, 99, 243, 323, 475
BmrFI	CCNGG	6 cut(s)	73, 220, 511, 596, 782, 783
BmsI	GCATC	5 cut(s)	26, 435, 454, 659, 803
BpmI	CTGGAG	1 cut(s)	733
Bpu10I	CCTNAGC	2 cut(s)	189, 738
Bpu14I	TTCGAA	1 cut(s)	422
BpuEI	CTTGAG	1 cut(s)	224
BpuMI	CCSGG	3 cut(s)	220, 782, 783
BsaAI	YACGTR	1 cut(s)	181
BsaBI	GATNNNNATC	1 cut(s)	567
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	7 cut(s)	72, 219, 357, 510, 594, 780, 781
BsaWI	WCCGGW	3 cut(s)	244, 260, 935
BsaXI	ACNNNNNCTCC	3 cut(s)	402, 432, 951
Bsc4I	CCNNNNNNNGG	2 cut(s)	72, 107
Bse118I	RCCGGY	1 cut(s)	44
Bse1I	ACTGG	4 cut(s)	82, 580, 897, 924
Bse3DI	GCAATG	1 cut(s)	98
Bse8I	GATNNNNATC	1 cut(s)	567
BseBI	CCWGG	3 cut(s)	73, 511, 596
BseDI	CCNNGG	7 cut(s)	72, 219, 357, 510, 594, 780, 781
BseGI	GGATG	3 cut(s)	520, 650, 969
BseJI	GATNNNNATC	1 cut(s)	567
BseLI	CCNNNNNNNGG	2 cut(s)	72, 107
BseMI	GCAATG	1 cut(s)	98
BseMII	CTCAG	1 cut(s)	203
BseNI	ACTGG	4 cut(s)	82, 580, 897, 924
BseRI	GAGGAG	3 cut(s)	151, 374, 426
BseSI	GKGCMC	1 cut(s)	100
BseXI	GCAGC	4 cut(s)	125, 611, 614, 685
BshFI	GGCC	6 cut(s)	77, 98, 356, 870, 908, 923
BsiHKAI	GWGCWC	2 cut(s)	61, 948
BsiHKCI	CYCGRG	1 cut(s)	781
BsiSI	CCGG	7 cut(s)	45, 117, 219, 245, 261, 782, 936
BslI	CCNNNNNNNGG	2 cut(s)	72, 107
BsmAI	GTCTC	1 cut(s)	839
BsnI	GGCC	6 cut(s)	77, 98, 356, 870, 908, 923
BsoBI	CYCGRG	1 cut(s)	781
Bsp119I	TTCGAA	1 cut(s)	422
Bsp120I	GGGCCC	1 cut(s)	96
Bsp1286I	GDGCHC	4 cut(s)	61, 100, 190, 948
Bsp143I	GATC	3 cut(s)	119, 149, 562
BspACI	CCGC	4 cut(s)	42, 63, 108, 920
BspANI	GGCC	6 cut(s)	77, 98, 356, 870, 908, 923
BspCNI	CTCAG	1 cut(s)	202
BspHI	TCATGA	1 cut(s)	612
BspLI	GGNNCC	5 cut(s)	98, 99, 243, 323, 475
BspPI	GGATC	2 cut(s)	127, 144
BspT104I	TTCGAA	1 cut(s)	422
BsrDI	GCAATG	1 cut(s)	98
BsrFI	RCCGGY	1 cut(s)	44
BsrI	ACTGG	4 cut(s)	82, 580, 897, 924
BssAI	RCCGGY	1 cut(s)	44
BssECI	CCNNGG	7 cut(s)	72, 219, 357, 510, 594, 780, 781
BssMI	GATC	3 cut(s)	119, 149, 562
BssNI	GRCGYC	1 cut(s)	69
Bst2UI	CCWGG	3 cut(s)	73, 511, 596
Bst4CI	ACNGT	3 cut(s)	478, 695, 733
BstACI	GRCGYC	1 cut(s)	69
BstBAI	YACGTR	1 cut(s)	181
BstBI	TTCGAA	1 cut(s)	422
BstC8I	GCNNGC	3 cut(s)	79, 210, 214
BstDEI	CTNAG	3 cut(s)	189, 738, 911
BstEII	GGTNACC	1 cut(s)	256
BstF5I	GGATG	3 cut(s)	520, 650, 969
BstH2I	RGCGCY	3 cut(s)	160, 219, 461
BstHHI	GCGC	4 cut(s)	113, 159, 218, 460
BstKTI	GATC	3 cut(s)	122, 152, 565
BstMAI	GTCTC	1 cut(s)	839
BstMBI	GATC	3 cut(s)	119, 149, 562
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 194, 224, 464, 704
BstNI	CCWGG	3 cut(s)	73, 511, 596
BstPI	GGTNACC	1 cut(s)	256
BstSCI	CCNGG	6 cut(s)	71, 218, 509, 594, 780, 781
BstSFI	CTRYAG	1 cut(s)	691
BstSLI	GKGCMC	1 cut(s)	100
BstV1I	GCAGC	4 cut(s)	125, 611, 614, 685
BstX2I	RGATCY	1 cut(s)	149
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	1 cut(s)	149
BsuRI	GGCC	6 cut(s)	77, 98, 356, 870, 908, 923
BtgZI	GCGATG	2 cut(s)	458, 834
BtsCI	GGATG	3 cut(s)	520, 650, 969
BtsIMutI	CAGTG	1 cut(s)	700
Cac8I	GCNNGC	3 cut(s)	79, 210, 214
CaiI	CAGNNNCTG	1 cut(s)	78
CciI	TCATGA	1 cut(s)	612
CfoI	GCGC	4 cut(s)	113, 159, 218, 460
Cfr10I	RCCGGY	1 cut(s)	44
Cfr13I	GGNCC	6 cut(s)	75, 96, 97, 242, 322, 907
Cfr9I	CCCGGG	1 cut(s)	781
CseI	GACGC	2 cut(s)	77, 208
Csp6I	GTAC	3 cut(s)	145, 485, 745
CviAII	CATG	3 cut(s)	613, 802, 931
CviQI	GTAC	3 cut(s)	145, 485, 745
DdeI	CTNAG	3 cut(s)	189, 738, 911
DpnI	GATC	3 cut(s)	121, 151, 564
DpnII	GATC	3 cut(s)	119, 149, 562
EaeI	YGGCCR	2 cut(s)	868, 921
EciI	GGCGGA	1 cut(s)	31
Ecl136II	GAGCTC	1 cut(s)	59
Eco24I	GRGCYC	3 cut(s)	61, 100, 190
Eco47I	GGWCC	2 cut(s)	242, 322
Eco47III	AGCGCT	2 cut(s)	158, 459
Eco53kI	GAGCTC	1 cut(s)	59
Eco57I	CTGAAG	1 cut(s)	125
Eco72I	CACGTG	1 cut(s)	181
Eco88I	CYCGRG	1 cut(s)	781
Eco91I	GGTNACC	1 cut(s)	256
EcoICRI	GAGCTC	1 cut(s)	59
EcoO109I	RGGNCCY	3 cut(s)	75, 97, 322
EcoO65I	GGTNACC	1 cut(s)	256
EcoRI	GAATTC	1 cut(s)	899
EcoRII	CCWGG	3 cut(s)	71, 509, 594
EcoT38I	GRGCYC	3 cut(s)	61, 100, 190
FaeI	CATG	3 cut(s)	616, 805, 934
FaiI	YATR	6 cut(s)	583, 589, 614, 647, 803, 932
FatI	CATG	3 cut(s)	612, 801, 930
FauI	CCCGC	1 cut(s)	70
Fnu4HI	GCNGC	7 cut(s)	15, 109, 114, 600, 603, 699, 921
FokI	GGATG	2 cut(s)	527, 637
FriOI	GRGCYC	3 cut(s)	61, 100, 190
Fsp4HI	GCNGC	7 cut(s)	15, 109, 114, 600, 603, 699, 921
FspBI	CTAG	3 cut(s)	270, 665, 815
GlaI	GCGC	4 cut(s)	112, 158, 217, 459
GluI	GCNGC	7 cut(s)	15, 109, 114, 600, 603, 699, 921
GsuI	CTGGAG	1 cut(s)	733
HaeII	RGCGCY	3 cut(s)	160, 219, 461
HaeIII	GGCC	6 cut(s)	77, 98, 356, 870, 908, 923
HapII	CCGG	7 cut(s)	45, 117, 219, 245, 261, 782, 936
HgaI	GACGC	2 cut(s)	77, 208
HhaI	GCGC	4 cut(s)	113, 159, 218, 460
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	3 cut(s)	616, 805, 934
Hin6I	GCGC	4 cut(s)	111, 157, 216, 458
HinP1I	GCGC	4 cut(s)	111, 157, 216, 458
HpaII	CCGG	7 cut(s)	45, 117, 219, 245, 261, 782, 936
HphI	GGTGA	2 cut(s)	268, 940
Hpy166II	GTNNAC	1 cut(s)	392
Hpy188I	TCNGA	4 cut(s)	129, 410, 723, 799
Hpy188III	TCNNGA	2 cut(s)	270, 613
Hpy8I	GTNNAC	1 cut(s)	392
Hpy99I	CGWCG	5 cut(s)	29, 32, 71, 202, 881
HpyAV	CCTTC	3 cut(s)	343, 622, 627
HpyCH4III	ACNGT	3 cut(s)	478, 695, 733
HpyCH4IV	ACGT	2 cut(s)	24, 180
HpyCH4V	TGCA	6 cut(s)	17, 318, 500, 591, 701, 794
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 194, 224, 464, 704
HpyF3I	CTNAG	3 cut(s)	189, 738, 911
HpySE526I	ACGT	2 cut(s)	24, 180
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	3 cut(s)	616, 805, 934
HspAI	GCGC	4 cut(s)	111, 157, 216, 458
Kzo9I	GATC	3 cut(s)	119, 149, 562
LmnI	GCTCC	5 cut(s)	56, 64, 129, 164, 263
Lsp1109I	GCAGC	4 cut(s)	125, 611, 614, 685
LweI	GCATC	5 cut(s)	26, 435, 454, 659, 803
MaeI	CTAG	3 cut(s)	270, 665, 815
MaeII	ACGT	2 cut(s)	24, 180
MaeIII	GTNAC	3 cut(s)	176, 256, 926
MalI	GATC	3 cut(s)	121, 151, 564
MboI	GATC	3 cut(s)	119, 149, 562
MboII	GAAGA	7 cut(s)	440, 443, 631, 634, 637, 844, 873
MfeI	CAATTG	1 cut(s)	288
MflI	RGATCY	1 cut(s)	149
MhlI	GDGCHC	4 cut(s)	61, 100, 190, 948
MluCI	AATT	7 cut(s)	50, 288, 331, 504, 714, 837, 899
MmeI	TCCRAC	3 cut(s)	107, 649, 658
MseI	TTAA	4 cut(s)	87, 330, 539, 768
MslI	CAYNNNNRTG	1 cut(s)	762
MspA1I	CMGCKG	1 cut(s)	920
MspI	CCGG	7 cut(s)	45, 117, 219, 245, 261, 782, 936
MspR9I	CCNGG	6 cut(s)	73, 220, 511, 596, 782, 783
MunI	CAATTG	1 cut(s)	288
MvaI	CCWGG	3 cut(s)	73, 511, 596
MwoI	GCNNNNNNNGC	5 cut(s)	11, 194, 224, 464, 704
NciI	CCSGG	3 cut(s)	220, 782, 783
NdeII	GATC	3 cut(s)	119, 149, 562
NlaIII	CATG	3 cut(s)	616, 805, 934
NlaIV	GGNNCC	5 cut(s)	98, 99, 243, 323, 475
NmeAIII	GCCGAG	1 cut(s)	382
NmuCI	GTSAC	2 cut(s)	176, 256
NspV	TTCGAA	1 cut(s)	422
PagI	TCATGA	1 cut(s)	612
PcsI	WCGNNNNNNNCGW	1 cut(s)	873
PkrI	GCNGC	7 cut(s)	16, 110, 115, 601, 604, 700, 922
PmaCI	CACGTG	1 cut(s)	181
PmlI	CACGTG	1 cut(s)	181
Ppu21I	YACGTR	1 cut(s)	181
PpuMI	RGGWCCY	1 cut(s)	322
Psp124BI	GAGCTC	1 cut(s)	61
Psp5II	RGGWCCY	1 cut(s)	322
Psp6I	CCWGG	3 cut(s)	71, 509, 594
PspCI	CACGTG	1 cut(s)	181
PspEI	GGTNACC	1 cut(s)	256
PspGI	CCWGG	3 cut(s)	71, 509, 594
PspN4I	GGNNCC	5 cut(s)	98, 99, 243, 323, 475
PspOMI	GGGCCC	1 cut(s)	96
PspPI	GGNCC	6 cut(s)	75, 96, 97, 242, 322, 907
PspPPI	RGGWCCY	1 cut(s)	322
PstNI	CAGNNNCTG	1 cut(s)	78
PsuI	RGATCY	1 cut(s)	149
RsaI	GTAC	3 cut(s)	146, 486, 746
RsaNI	GTAC	3 cut(s)	145, 485, 745
RseI	CAYNNNNRTG	1 cut(s)	762
SacI	GAGCTC	1 cut(s)	61
SaqAI	TTAA	4 cut(s)	87, 330, 539, 768
SatI	GCNGC	7 cut(s)	15, 109, 114, 600, 603, 699, 921
Sau3AI	GATC	3 cut(s)	119, 149, 562
Sau96I	GGNCC	6 cut(s)	75, 96, 97, 242, 322, 907
ScaI	AGTACT	1 cut(s)	746
ScrFI	CCNGG	6 cut(s)	73, 220, 511, 596, 782, 783
SduI	GDGCHC	4 cut(s)	61, 100, 190, 948
SfaNI	GCATC	5 cut(s)	26, 435, 454, 659, 803
SfcI	CTRYAG	1 cut(s)	691
SfuI	TTCGAA	1 cut(s)	422
SinI	GGWCC	2 cut(s)	242, 322
SmaI	CCCGGG	1 cut(s)	783
SmiMI	CAYNNNNRTG	1 cut(s)	762
SmlI	CTYRAG	1 cut(s)	203
SmoI	CTYRAG	1 cut(s)	203
Sse9I	AATT	7 cut(s)	50, 288, 331, 504, 714, 837, 899
SsiI	CCGC	4 cut(s)	42, 63, 108, 920
SspMI	CTAG	3 cut(s)	270, 665, 815
SstI	GAGCTC	1 cut(s)	61
StyD4I	CCNGG	6 cut(s)	71, 218, 509, 594, 780, 781
TaaI	ACNGT	3 cut(s)	478, 695, 733
TaiI	ACGT	2 cut(s)	27, 183
TaqI	TCGA	2 cut(s)	347, 422
TasI	AATT	7 cut(s)	50, 288, 331, 504, 714, 837, 899
TatI	WGTACW	3 cut(s)	144, 484, 744
TauI	GCSGC	2 cut(s)	111, 923
Tru1I	TTAA	4 cut(s)	87, 330, 539, 768
Tru9I	TTAA	4 cut(s)	87, 330, 539, 768
TscAI	CASTG	1 cut(s)	700
TseFI	GTSAC	2 cut(s)	176, 256
TseI	GCWGC	5 cut(s)	14, 113, 599, 602, 698
Tsp45I	GTSAC	2 cut(s)	176, 256
TspDTI	ATGAA	2 cut(s)	548, 632
TspGWI	ACGGA	1 cut(s)	643
TspMI	CCCGGG	1 cut(s)	781
TspRI	CASTG	1 cut(s)	700
VpaK11BI	GGWCC	2 cut(s)	242, 322
XapI	RAATTY	3 cut(s)	50, 837, 899
XbaI	TCTAGA	1 cut(s)	269
XmaI	CCCGGG	1 cut(s)	781
XspI	CTAG	3 cut(s)	270, 665, 815
ZrmI	AGTACT	1 cut(s)	746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.