Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003182

Physical Location & Seq

Genomic Coordinates Forward (+)

25689289 .. 25691588

Gene Structure 2300 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1162300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGCGGCCTCGATCGCCTTCAACTCCGTCGGAGCTCCGGCGCGACTCTCCCGCCAATTCTCCCACCCGCGAAGCTCCTATCCGCTTCTCGGCGGGCTCCGACTGCGCCACGCCTCTCCGGCCATGTTTTCCAGCTCCTTGTCTTCCTCCTCTGGCTCTGGAATTAGGGCACAGGTGGCGGACGTTGAGCAGTCTAGCGCTGCTGTGTCTCAGAATCCAAAGGTGGAGGCTCCGGTGGTTATCGTCACCGGAGCTTCTAGAGGAATCGGCAAGGCTGTTGCTCTCGCCCTCGGCAAGTCTGGTTGTAAGGTGTTGGTAAACTACGCAAGATCATCCAAGGAAGCTGAACAAGTTTCTAAGGAGATTGAGGCATCTGGTGGTCAAGCTCTAACATTTGGGGGTGATGTTTCAAAGGAAGAAGATGTCACAGCAATGATTAAGACCGTGGTCGATGCGTGGGGAACGGTTGATGTCTTGGTAAATAATGCAGGAATTACACGGGATGGCTTATTGATGAGAATGAAAAAATCCCAATGGCAGGAGGTTATTGATTTGAATCTTACTGGCGTGTTTCTATGCACGCAGGCTGCGGCAAAAATTATGATGAAACAGCGAAAGGGAAGGATAATCAATATAGCATCTGTTGTTGGTCTTGTTGGCAATGTTGGACAAGCCAACTACAGTGCTGCAAAGGCTGGAGTCATTGGCTTCACGAAGAGTGTTGCAAAGGAATATTCGAGCAGGAACATTAATGTCAATGCTGTTGCCCCTGGTTTTATCGCATCTGATATGACTTCCAAGCTTGGAGAAGACATAGAAAAGAAGATCTTGGGGAGCATCCCCTTAGGGCGATATGGTCAACCTGAAGAAGTGGCTGGACTGGTGGAATTCTTGGCGCTTAATCCCGCTGCCAGCTACATCACCGGACAGGTCTTGACCATTGATGGAGGGATGGTGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko00333 ko00780 ko01040 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01040 map01100 map01130 map01212

Pfam Domains

Protein Families

adh_short adh_short_C2

Protein Analysis

321

Amino Acids

33.33

Weight (kDa)

9.45

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	79 - 271	7.3e-67	short chain dehydrogenase
KR	PF08659	80 - 252	2e-21	KR domain
adh_short_C2	PF13561	84 - 318	3.8e-65	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24360
fragaria_vesca	FvH4_4g24340 FvH4_5g07680 FvH4_5g07680
malus_domestica	MD13G1112000.v1.1 MD14G1162300.v1.1 MD16G1111600.v1.1
prunus_persica	Prupe.1G233400_v2.0.a1 Prupe.3G006500_v2.0.a1 Prupe.3G006500_v2.0.a1 Prupe.5G154900_v2.0.a1 Prupe.5G154900_v2.0.a1 Prupe.5G154900_v2.0.a1
pyrus_communis	pycom13g09740 pycom14g13520 pycom16g09460
rosa_chinensis	RchiOBHm_Chr4g0431201 RchiOBHm_Chr7g0190791
rosa_laevigata	RLG00000004498 RLG00000006920
rosa_multiflora	Rmu_sc0005258.1_g000006 Rmu_sc0032787.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264310 Rroxscaffold_5G00372740
rosa_rugosa	Rorug04G0249100 Rorug06G0506900
rosa_samantha	Rh4AG305200 Rh4BG312500 Rh4CG328800 Rh4DG309100 Rh7AG113600 Rh7BG116100 Rh7CG118700 Rh7CG119000 Rh7DG117600
rosa_wichuraiana	Rw4G026520 Rw7G009920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	43, 70
AciI	CCGC	8 cut(s)	5, 52, 68, 83, 93, 179, 590, 906
AcoI	YGGCCR	1 cut(s)	120
AcsI	RAATTY	1 cut(s)	887
AcuI	CTGAAG	1 cut(s)	885
AfeI	AGCGCT	1 cut(s)	199
AfiI	CCNNNNNNNGG	2 cut(s)	89, 538
AgsI	TTSAA	3 cut(s)	22, 411, 556
AjnI	CCWGG	1 cut(s)	769
AluBI	AGCT	8 cut(s)	35, 75, 135, 254, 344, 386, 802, 915
AluI	AGCT	8 cut(s)	35, 75, 135, 254, 344, 386, 802, 915
Alw21I	GWGCWC	1 cut(s)	37
Alw26I	GTCTC	1 cut(s)	213
Aor51HI	AGCGCT	1 cut(s)	199
AoxI	GGCC	2 cut(s)	6, 120
ApeKI	GCWGC	4 cut(s)	200, 587, 686, 908
ApoI	RAATTY	1 cut(s)	887
AseI	ATTAAT	1 cut(s)	750
AspLEI	GCGC	4 cut(s)	43, 108, 200, 898
AsuHPI	GGTGA	3 cut(s)	238, 413, 913
AxyI	CCTNAGG	1 cut(s)	844
BaeGI	GKGCMC	1 cut(s)	172
BanII	GRGCYC	2 cut(s)	37, 99
BbsI	GAAGAC	2 cut(s)	135, 816
Bbv12I	GWGCWC	1 cut(s)	37
BbvI	GCAGC	4 cut(s)	187, 574, 673, 895
BccI	CCATC	3 cut(s)	497, 938, 946
BciT130I	CCWGG	1 cut(s)	771
BcoDI	GTCTC	1 cut(s)	213
BfaI	CTAG	2 cut(s)	195, 258
BfmI	CTRYAG	1 cut(s)	679
BfoI	RGCGCY	2 cut(s)	201, 899
BglI	GCCNNNNNGGC	1 cut(s)	119
BglII	AGATCT	1 cut(s)	825
BisI	GCNGC	6 cut(s)	6, 201, 588, 591, 687, 909
BlsI	GCNGC	6 cut(s)	7, 202, 589, 592, 688, 910
Bme1390I	CCNGG	1 cut(s)	771
BmiI	GGNNCC	2 cut(s)	98, 231
BmrFI	CCNGG	1 cut(s)	771
BmsI	GCATC	5 cut(s)	380, 442, 647, 791, 846
BoxI	GACNNNNGTC	1 cut(s)	446
BpiI	GAAGAC	2 cut(s)	135, 816
BpmI	CTGGAG	1 cut(s)	717
BsaBI	GATNNNNATC	1 cut(s)	555
BsaJI	CCNNGG	4 cut(s)	289, 336, 444, 769
BsaWI	WCCGGW	3 cut(s)	232, 248, 923
BsaXI	ACNNNNNCTCC	1 cut(s)	939
Bsc4I	CCNNNNNNNGG	2 cut(s)	89, 538
Bse1I	ACTGG	2 cut(s)	568, 885
Bse21I	CCTNAGG	1 cut(s)	844
Bse3DI	GCAATG	2 cut(s)	438, 667
Bse8I	GATNNNNATC	1 cut(s)	555
BseBI	CCWGG	1 cut(s)	771
BseDI	CCNNGG	4 cut(s)	289, 336, 444, 769
BseGI	GGATG	4 cut(s)	332, 508, 837, 957
BseJI	GATNNNNATC	1 cut(s)	555
BseLI	CCNNNNNNNGG	2 cut(s)	89, 538
BseMI	GCAATG	2 cut(s)	438, 667
BseMII	CTCAG	1 cut(s)	224
BseNI	ACTGG	2 cut(s)	568, 885
BseRI	GAGGAG	1 cut(s)	139
BseSI	GKGCMC	1 cut(s)	172
BseXI	GCAGC	4 cut(s)	187, 574, 673, 895
Bsh1236I	CGCG	2 cut(s)	43, 70
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	2 cut(s)	8, 122
BsiEI	CGRYCG	1 cut(s)	15
BsiHKAI	GWGCWC	1 cut(s)	37
BsiSI	CCGG	5 cut(s)	38, 119, 233, 249, 924
BslI	CCNNNNNNNGG	2 cut(s)	89, 538
BsmAI	GTCTC	1 cut(s)	213
BsnI	GGCC	2 cut(s)	8, 122
Bsp1286I	GDGCHC	3 cut(s)	37, 99, 172
Bsp143I	GATC	3 cut(s)	12, 329, 825
BspACI	CCGC	8 cut(s)	5, 52, 68, 83, 93, 179, 590, 906
BspANI	GGCC	2 cut(s)	8, 122
BspCNI	CTCAG	1 cut(s)	223
BspFNI	CGCG	2 cut(s)	43, 70
BspLI	GGNNCC	2 cut(s)	98, 231
BsrDI	GCAATG	2 cut(s)	438, 667
BsrI	ACTGG	2 cut(s)	568, 885
BssECI	CCNNGG	4 cut(s)	289, 336, 444, 769
BssMI	GATC	3 cut(s)	12, 329, 825
BssT1I	CCWWGG	1 cut(s)	336
Bst2UI	CCWGG	1 cut(s)	771
Bst4CI	ACNGT	3 cut(s)	445, 466, 683
Bst6I	CTCTTC	1 cut(s)	710
BstC8I	GCNNGC	4 cut(s)	95, 581, 585, 913
BstDEI	CTNAG	3 cut(s)	210, 357, 844
BstDSI	CCRYGG	1 cut(s)	444
BstF5I	GGATG	4 cut(s)	332, 508, 837, 957
BstFNI	CGCG	2 cut(s)	43, 70
BstH2I	RGCGCY	2 cut(s)	201, 899
BstHHI	GCGC	4 cut(s)	43, 108, 200, 898
BstKTI	GATC	3 cut(s)	15, 332, 828
BstMAI	GTCTC	1 cut(s)	213
BstMBI	GATC	3 cut(s)	12, 329, 825
BstMCI	CGRYCG	1 cut(s)	15
BstMWI	GCNNNNNNNGC	5 cut(s)	14, 103, 119, 176, 692
BstNI	CCWGG	1 cut(s)	771
BstPAI	GACNNNNGTC	1 cut(s)	446
BstSCI	CCNGG	1 cut(s)	769
BstSFI	CTRYAG	1 cut(s)	679
BstSLI	GKGCMC	1 cut(s)	172
BstUI	CGCG	2 cut(s)	43, 70
BstV1I	GCAGC	4 cut(s)	187, 574, 673, 895
BstV2I	GAAGAC	2 cut(s)	135, 816
BstX2I	RGATCY	1 cut(s)	825
BstYI	RGATCY	1 cut(s)	825
Bsu36I	CCTNAGG	1 cut(s)	844
BsuRI	GGCC	2 cut(s)	8, 122
BtgI	CCRYGG	1 cut(s)	444
BtsCI	GGATG	4 cut(s)	332, 508, 837, 957
BtsIMutI	CAGTG	1 cut(s)	688
Cac8I	GCNNGC	4 cut(s)	95, 581, 585, 913
CfoI	GCGC	4 cut(s)	43, 108, 200, 898
CviAII	CATG	1 cut(s)	124
DdeI	CTNAG	3 cut(s)	210, 357, 844
DpnI	GATC	3 cut(s)	14, 331, 827
DpnII	GATC	3 cut(s)	12, 329, 825
EaeI	YGGCCR	1 cut(s)	120
Eam1104I	CTCTTC	1 cut(s)	710
EarI	CTCTTC	1 cut(s)	710
EciI	GGCGGA	1 cut(s)	194
Ecl136II	GAGCTC	1 cut(s)	35
Eco130I	CCWWGG	1 cut(s)	336
Eco24I	GRGCYC	2 cut(s)	37, 99
Eco47III	AGCGCT	1 cut(s)	199
Eco53kI	GAGCTC	1 cut(s)	35
Eco57I	CTGAAG	1 cut(s)	885
Eco81I	CCTNAGG	1 cut(s)	844
EcoICRI	GAGCTC	1 cut(s)	35
EcoRI	GAATTC	1 cut(s)	887
EcoRII	CCWGG	1 cut(s)	769
EcoT14I	CCWWGG	1 cut(s)	336
EcoT38I	GRGCYC	2 cut(s)	37, 99
ErhI	CCWWGG	1 cut(s)	336
FaeI	CATG	1 cut(s)	127
FaiI	YATR	7 cut(s)	125, 577, 602, 635, 791, 815, 855
FalI	AAGNNNNNCTT	2 cut(s)	812, 844
FatI	CATG	1 cut(s)	123
FauI	CCCGC	4 cut(s)	59, 75, 86, 913
Fnu4HI	GCNGC	6 cut(s)	6, 201, 588, 591, 687, 909
FokI	GGATG	3 cut(s)	319, 515, 824
FriOI	GRGCYC	2 cut(s)	37, 99
Fsp4HI	GCNGC	6 cut(s)	6, 201, 588, 591, 687, 909
FspBI	CTAG	2 cut(s)	195, 258
GlaI	GCGC	4 cut(s)	42, 107, 199, 897
GluI	GCNGC	6 cut(s)	6, 201, 588, 591, 687, 909
GsuI	CTGGAG	1 cut(s)	717
HaeII	RGCGCY	2 cut(s)	201, 899
HaeIII	GGCC	2 cut(s)	8, 122
HapII	CCGG	5 cut(s)	38, 119, 233, 249, 924
HhaI	GCGC	4 cut(s)	43, 108, 200, 898
Hin1II	CATG	1 cut(s)	127
Hin6I	GCGC	4 cut(s)	41, 106, 198, 896
HinP1I	GCGC	4 cut(s)	41, 106, 198, 896
HincII	GTYRAC	1 cut(s)	860
HindII	GTYRAC	1 cut(s)	860
HindIII	AAGCTT	1 cut(s)	800
HinfI	GANTC	5 cut(s)	45, 214, 264, 556, 699
HpaII	CCGG	5 cut(s)	38, 119, 233, 249, 924
HphI	GGTGA	3 cut(s)	238, 413, 913
Hpy166II	GTNNAC	2 cut(s)	319, 860
Hpy188I	TCNGA	4 cut(s)	32, 101, 213, 787
Hpy188III	TCNNGA	4 cut(s)	159, 258, 712, 934
Hpy8I	GTNNAC	2 cut(s)	319, 860
Hpy99I	CGWCG	1 cut(s)	32
HpyAV	CCTTC	2 cut(s)	28, 615
HpyCH4III	ACNGT	3 cut(s)	445, 466, 683
HpyCH4IV	ACGT	1 cut(s)	183
HpyCH4V	TGCA	4 cut(s)	488, 579, 689, 725
HpyF10VI	GCNNNNNNNGC	5 cut(s)	14, 103, 119, 176, 692
HpyF3I	CTNAG	3 cut(s)	210, 357, 844
HpySE526I	ACGT	1 cut(s)	183
Hsp92II	CATG	1 cut(s)	127
HspAI	GCGC	4 cut(s)	41, 106, 198, 896
Kzo9I	GATC	3 cut(s)	12, 329, 825
LmnI	GCTCC	8 cut(s)	32, 40, 80, 102, 140, 235, 251, 834
Lsp1109I	GCAGC	4 cut(s)	187, 574, 673, 895
LweI	GCATC	5 cut(s)	380, 442, 647, 791, 846
MaeI	CTAG	2 cut(s)	195, 258
MaeII	ACGT	1 cut(s)	183
MaeIII	GTNAC	2 cut(s)	244, 424
MalI	GATC	3 cut(s)	14, 331, 827
MboI	GATC	3 cut(s)	12, 329, 825
MboII	GAAGA	7 cut(s)	135, 428, 431, 727, 821, 835, 878
MflI	RGATCY	1 cut(s)	825
MhlI	GDGCHC	3 cut(s)	37, 99, 172
MluCI	AATT	5 cut(s)	56, 162, 492, 597, 887
MlyI	GAGTC	2 cut(s)	39, 708
MmeI	TCCRAC	3 cut(s)	10, 124, 646
MseI	TTAA	3 cut(s)	438, 750, 900
MslI	CAYNNNNRTG	1 cut(s)	431
MspA1I	CMGCKG	1 cut(s)	908
MspI	CCGG	5 cut(s)	38, 119, 233, 249, 924
MspR9I	CCNGG	1 cut(s)	771
MvaI	CCWGG	1 cut(s)	771
MvnI	CGCG	2 cut(s)	43, 70
MwoI	GCNNNNNNNGC	5 cut(s)	14, 103, 119, 176, 692
NdeII	GATC	3 cut(s)	12, 329, 825
NlaIII	CATG	1 cut(s)	127
NlaIV	GGNNCC	2 cut(s)	98, 231
NmeAIII	GCCGAG	2 cut(s)	69, 270
NmuCI	GTSAC	2 cut(s)	244, 424
PfeI	GAWTC	3 cut(s)	214, 264, 556
PkrI	GCNGC	6 cut(s)	7, 202, 589, 592, 688, 910
Ple19I	CGATCG	1 cut(s)	15
PleI	GAGTC	2 cut(s)	39, 707
PpsI	GAGTC	2 cut(s)	39, 707
PshAI	GACNNNNGTC	1 cut(s)	446
PshBI	ATTAAT	1 cut(s)	750
Psp124BI	GAGCTC	1 cut(s)	37
Psp6I	CCWGG	1 cut(s)	769
PspGI	CCWGG	1 cut(s)	769
PspN4I	GGNNCC	2 cut(s)	98, 231
PsuI	RGATCY	1 cut(s)	825
PvuI	CGATCG	1 cut(s)	15
RseI	CAYNNNNRTG	1 cut(s)	431
SacI	GAGCTC	1 cut(s)	37
SaqAI	TTAA	3 cut(s)	438, 750, 900
SatI	GCNGC	6 cut(s)	6, 201, 588, 591, 687, 909
Sau3AI	GATC	3 cut(s)	12, 329, 825
SchI	GAGTC	2 cut(s)	39, 708
ScrFI	CCNGG	1 cut(s)	771
SduI	GDGCHC	3 cut(s)	37, 99, 172
SfaNI	GCATC	5 cut(s)	380, 442, 647, 791, 846
SfcI	CTRYAG	1 cut(s)	679
SmiMI	CAYNNNNRTG	1 cut(s)	431
Sse9I	AATT	5 cut(s)	56, 162, 492, 597, 887
SsiI	CCGC	8 cut(s)	5, 52, 68, 83, 93, 179, 590, 906
SspI	AATATT	1 cut(s)	734
SspMI	CTAG	2 cut(s)	195, 258
SstI	GAGCTC	1 cut(s)	37
StyD4I	CCNGG	1 cut(s)	769
StyI	CCWWGG	1 cut(s)	336
TaaI	ACNGT	3 cut(s)	445, 466, 683
TaiI	ACGT	1 cut(s)	186
TaqI	TCGA	3 cut(s)	11, 450, 737
TasI	AATT	5 cut(s)	56, 162, 492, 597, 887
TauI	GCSGC	2 cut(s)	8, 593
TfiI	GAWTC	3 cut(s)	214, 264, 556
Tru1I	TTAA	3 cut(s)	438, 750, 900
Tru9I	TTAA	3 cut(s)	438, 750, 900
TscAI	CASTG	1 cut(s)	688
TseFI	GTSAC	2 cut(s)	244, 424
TseI	GCWGC	4 cut(s)	200, 587, 686, 908
Tsp45I	GTSAC	2 cut(s)	244, 424
TspDTI	ATGAA	2 cut(s)	536, 620
TspGWI	ACGGA	1 cut(s)	16
TspRI	CASTG	1 cut(s)	688
VspI	ATTAAT	1 cut(s)	750
XapI	RAATTY	1 cut(s)	887
XbaI	TCTAGA	1 cut(s)	257
XspI	CTAG	2 cut(s)	195, 258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.