MD13G1175000.v1.1

Regulator of Ty1 transposition protein 107 BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007475
Physical Location & Seq
Forward (+)
14445370 .. 14451088
5719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1175000.v1.1.491

Sequence Viewer

Length: 3741 bp
ATGGGTTCTCTCTGGAACGGCGAAGATGAAATCATGCCGATGGAGACAAACTCACTCGCCGATTGCGCAGACACCGAGCCCATTGACACTCAGATTTCAAGCCCTCCATCGTCCGATGAAAAGGGCAAATCGAGGGATGCGGATGAGTTAGTGCGAGACACCGTGCCTTGTAATGACACTGTTCCAGTTGAAGATGCATTTGAAACCCAAATGGTAGATTTTGGTGGTGAAACCCAATTAATAGATTTCGGTGGTGAAACCCAATTAATGGATTTTGGTTGGGAAACCCAAGTAATGGATTTTGATGGCGAAACCCAAGTAATGGATTTTGGTGGGGACACACAAGTGATGGATTTCGGCGGTGATACACAAGCAATGGGTTTTGGTGGTGAAACACAAGTAATGGATGATATCAACTGCTATGCGAACATGGAGGAGACCCAGTTATTAGAGTTTGATGATGTAGTTGTCAGTGCCACTGACAGTGAGGAGTCGGATACAACTGAAGTTTTGGATGATAGTAAGGATCTAAGTGATGATGAATCAGTACGGAGAGGTTCTGGTCAATTAGTGAATGAGGAGAATATTAGCCGCACTCCTTGTGAAAATAGTGTAAATGGGTTAATGGAGCAAGCCAATTATTCGGTCGACAATCAACACAATGCAGGGCTCCATGTTTCTGCTGCAACACCTGTGGTTGAAGGCTCCCCGGAACTGAGACCAGGTTCTGTGCATAAGCATTTTACCTCGGTTCGTGCAGCATCCTTACAGGCCTCTGGTCTAGCAGCTTGCAGTATGGTTTTGAAGGGCACCAACAATAAGTCTTGTTCTGTTAGAAGTAATAATCAGTCTTTGGATCAACTTTCCGGTAAAGATAATGCAGTATCTCTTCTAGGGGGTTCAGCCGTAAATGGAGACGAAGTCAGGCAGGAACATGGTTCAAGGAATGAGAAAAAATGCAGGACTGGAAGTTCAACAGCGAGAAATCTTTTTCCTGAGGATTCAGATGCTGAAAACACAGAAATTTCTCATCACAGCGGTAGTGGTGAAGAAGGGGAAGATTTGCTTCAGTTTCCTTGTAATTTGGTAGGGTTAAGCTATGTTGACTCTCAAGAACCAGGTGAATTGTCACAAGCTAATGCACTTAATTTTGTGGATAAGTTCCTCCAAAATAATCTTGATGAGTCTAATAAAGAAGTTGGCCATGGAAAGAGAGCTAGGGACACTTCAAAATTTATCTCAAGTGCAAAAGGGCCACAAATATTGGCTAAGAAAGCCAATGACAAAAGCATAGATAAAGGGATTTTTGATTGGGATAATAACCGAGAGGATGAAGAAGGAGGTGAATTTTTCCGCCGAAGAAAAGCAGACTTCTTTGATGGTGGAAGCCATGGGTGGAGATCTCTTCCCCAACCCCAGAAGTCTAAAGGGAAAAGACAGGAGGAGAAGAAGGACCTCAAAACACAATTACAAGGAAAGAACAAGAGAATAGGTGTAGTTCATTCTGATTCAAAACTATTGTTGCATAAATCAAAAGTCGACAAGAAGACAGCACATGAAGATGAAATGAAGCATATAAAGAATCTTGTCAGTGAGTTCGACGAACAGTTCAACAATGACTCCCCAAGAGAGCAGTTGGATACTAATGTTAATAAGAATGCGCCTGAAATGATGAATGTAGGCTTTGATACTCAAATGGCAGCTGAAGCTATAGAAGCCTTGTGTTGTGGTGTAGGGATTTCCAATTGTGATGCTAGTGATGAAAATCAAGGTGCTGGAAGAAAGCAAAAGAGTCCTCCTGAAGGTTCGATGGGAGAAAAATCAAAGAACAGAATTTGTTCAACGAAACCTTCTTCTCGAAAAAGAGGGCGTTTTACTGATACAGGGGTTGCTTCTCGAGAAACTCGACAAGCCAAGAAAACAAGGGTTGGTGCCAGATTATGCAAACATTATTCAATTTCACCTCTGGAATTTTCTAAGAATGCTAGGAAACAGTGTGAAACAGAGGTGGTGATAACAAAATCAAAGAAGGGGAAGTCCATTGCTAAAAATCATCTTAAAATTGACAGGAACAAAAACATGGAGAAAATACCTTCTGTTGCTATTGACCTAAGAACAGAAAAAAAGAATTTACAACAGGATGTTGGTACTTTTACACCCATTGCATGTCGAACAAGACGGTCTATGGTGGTAAATCAGTTAAAGAAAGCTGACAAAGCATCCAGTGATTGTGGAGAAGAGTCAAGCGCTCAAACACAGTATATTGCAACTAGAGAGAAAATATTTAGTTTCACAGGTGGTAAGGTGTCTAATGCGTTGAATGCAAAGCTTTCAAAATTGGGTCCCAGTCGATCCGGAGAAGTTGGAACTAATAAACGAAGCCAACATGATGGTTCAGATCTCAAATTTGAAGCTATTTGCGATGGCATCAAATTGGATACTTTAAGCTTCTCCAAAGGAAAAAGATCTAGGAGAAAGTTATCTGATCAGGTTTGTGGGCCTGATAACTTAAATGATCCATCTGCCCCATTTGCTCAGCCAGACAAAGTTGGACAGCGTTTCAGCAGGCACACAAGATCGCAGGGTGCTGCTCAAAGAATTTGTGTTGATGTAAAATCAACAAGAAGAACCCGATCATGTACACGTGGAGACCAGAATTTGGCTAGAAAGTATGCACATCAAAGTTTAAAAGGTGATCCTGGGAAAGTTCCTCTTCATTGTAATTCTACTCACAAGAATGGGAGAATGATATCAGAGATTCTAACTGGTGAAGAGGCAGTTGGAATTCCTGACAGGAGGAGTGGTGCAAATCCATCTTCTACTACTAAAATGAGGGATGAATCCCCACTAGGGAAATGCAAGCCATCGGATTCAGGTTGTGCTACTCCAGTTAACAGTAAGGTGCCTGCCAATGATGCATCTCCAGTTTGTATGGGTAGTGAATATTTCAAACAGACATGCAAGAAGACCCCTTCAAGGCCAGGACTTTCAAAAGAAATCCGTGACTTAAGTGCAAACGGGAATACACCAACTTCAGCAAAGAGAGATAGGACTGATGTTCGAGTATTGTATAGCCACCACTTGGATGACAATGTAATTAAGCATCAGAAAAAGATTTTGGCCAGACTAGGAGTTTCTGTAGCATCCTCTATGACAGATGCCACGCACTTCATAGCAGATCAATTCATGCGTACAAGGAATATGTTAGAAGCTATTGCTGCTGGAAAACCAGTGGTAACGCATTTATGGCTCGATAGCTGTGGACAAGCTAGTTGTTTCATCGATGAGAAAAACTATATACTAAGGGATACTAAGAAGGAGAAGGAGTTCGGCTTTAGCATGCCAGCTTCGTTGGCACGTGCGTGCCAGCATCCACTTCTGGAGGGTCGTAAAGTCTTCATCACCCCAAATACGAAGCCTGGTAAAGATATAATTTCAAGTCTGGTCAAAGCAGTCCGTGGCCAGGCTATAGAAAGAATTGGTAGATCTGTTTTGGAGGCTGATAAAATTCCTGATGATCTGTTGGTTGTATCTTGTGAAGAAGATTATGAAATCTGTGTGCCTTTACTCGAAAAAGGAGCTACAGTTTACAGCTCAGAGTTGCTATTGAATGGGATTGTTACTCAGAAGCTGAAGTTTGAAAGGCATCGCCTCTTTACAGATCAAGTTAAGAAAACCCGTTCCACCATATGGCTGAGGAAAGATGGCAACAAGTTCCTCCCTGTGACGAAAGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000414 GO:0000416 GO:0000726 GO:0002637 GO:0002639 GO:0002682 GO:0002684 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002703 GO:0002705 GO:0002706 GO:0002708 GO:0002712 GO:0002714 GO:0002819 GO:0002821 GO:0002822 GO:0002824 GO:0002889 GO:0002891 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006303 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030330 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034708 GO:0034968 GO:0035065 GO:0035066 GO:0035097 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043414 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044666 GO:0045191 GO:0045830 GO:0045893 GO:0045911 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0050867 GO:0050871 GO:0050896 GO:0051052 GO:0051054 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051249 GO:0051251 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0051569 GO:0051571 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072331 GO:0080090 GO:0090304 GO:1901360 GO:1901564 GO:1901983 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903320 GO:1903322 GO:1903506 GO:1903508 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000142 GO:2000144 GO:2000756 GO:2000758 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RTT107_BRCT_5

Protein Analysis

1247

Amino Acids

137.14

Weight (kDa)

6.85

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTT107_BRCT_5 PF16770 1009 - 1101 3e-10 Regulator of Ty1 transposition protein 107 BRCT domain
BRCT_2 PF16589 1125 - 1212 1.9e-16 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 67
AccB1I GGYRCC 3 cut(s) 809, 1931, 2902
AccB7I CCANNNNNTGG 6 cut(s) 268, 295, 322, 2659, 3082, 3690
AccI GTMKAC 2 cut(s) 648, 1539
AccIII TCCGGA 1 cut(s) 2354
AciI CCGC 5 cut(s) 140, 360, 592, 1038, 1354
AclWI GGATC 5 cut(s) 534, 864, 2346, 2510, 2690
AcoI YGGCCR 3 cut(s) 1201, 3120, 3460
AcuI CTGAAG 6 cut(s) 525, 1052, 1725, 1821, 3018, 3653
AcvI CACGTG 2 cut(s) 2645, 3359
AfaI GTAC 4 cut(s) 549, 2149, 2641, 3193
AfeI AGCGCT 1 cut(s) 2248
AflII CTTAAG 1 cut(s) 3007
AflIII ACRYGT 1 cut(s) 2642
AjnI CCWGG 6 cut(s) 721, 1117, 2698, 2980, 3418, 3462
AjuI GAANNNNNNNTTGG 4 cut(s) 2763, 2795, 3101, 3133
AleI CACNNNNGTG 2 cut(s) 344, 3361
AloI GAACNNNNNNTCC 4 cut(s) 955, 987, 3311, 3343
Alw26I GTCTC 6 cut(s) 38, 150, 431, 712, 909, 2643
AlwI GGATC 5 cut(s) 534, 864, 2346, 2510, 2690
AlwNI CAGNNNCTG 3 cut(s) 728, 1010, 3631
Ama87I CYCGRG 1 cut(s) 1896
Aor13HI TCCGGA 1 cut(s) 2354
Aor51HI AGCGCT 1 cut(s) 2248
AoxI GGCC 7 cut(s) 771, 1201, 1253, 2498, 2978, 3120, 3460
ApeKI GCWGC 6 cut(s) 683, 758, 785, 1700, 2588, 3218
AseI ATTAAT 2 cut(s) 239, 266
Asp700I GAANNNNTTC 2 cut(s) 1837, 3326
AspLEI GCGC 3 cut(s) 68, 1663, 2249
AspS9I GGNCC 4 cut(s) 1253, 1453, 2342, 2498
AsuC2I CCSGG 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 1896
AvaII GGWCC 2 cut(s) 1453, 2342
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 1 cut(s) 812
BaeI ACNNNNGTAYC 4 cut(s) 1632, 1665, 1872, 1905
BalI TGGCCA 3 cut(s) 1203, 3122, 3462
BanI GGYRCC 3 cut(s) 809, 1931, 2902
BanII GRGCYC 2 cut(s) 81, 672
BbrPI CACGTG 2 cut(s) 2645, 3359
BbsI GAAGAC 3 cut(s) 1553, 2972, 3388
BbvCI CCTCAGC 1 cut(s) 3695
BbvI GCAGC 6 cut(s) 670, 770, 797, 1712, 2575, 3205
BceAI ACGGC 2 cut(s) 34, 890
BciT130I CCWGG 6 cut(s) 723, 1119, 2700, 2982, 3420, 3464
BciVI GTATCC 4 cut(s) 490, 1633, 2431, 3301
BclI TGATCA 1 cut(s) 2485
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 6 cut(s) 38, 150, 431, 712, 909, 2643
BfmI CTRYAG 4 cut(s) 1710, 3138, 3468, 3582
BfoI RGCGCY 1 cut(s) 2250
BfrI CTTAAG 1 cut(s) 3007
BfuI GTATCC 4 cut(s) 490, 1633, 2431, 3301
BglII AGATCT 4 cut(s) 1400, 2398, 2465, 3485
BisI GCNGC 7 cut(s) 592, 684, 759, 786, 1701, 2589, 3219
BlpI GCTNAGC 1 cut(s) 2535
BlsI GCNGC 7 cut(s) 593, 685, 760, 787, 1702, 2590, 3220
Bme1390I CCNGG 7 cut(s) 710, 723, 1119, 2700, 2982, 3420, 3464
Bme18I GGWCC 2 cut(s) 1453, 2342
BmeT110I CYCGRG 1 cut(s) 1896
BmgT120I GGNCC 4 cut(s) 1253, 1453, 2342, 2498
BmiI GGNNCC 7 cut(s) 671, 706, 811, 1933, 2343, 2344, 2904
BmrFI CCNGG 7 cut(s) 710, 723, 1119, 2700, 2982, 3420, 3464
BmrI ACTGGG 2 cut(s) 436, 2340
BmuI ACTGGG 2 cut(s) 436, 2340
BpiI GAAGAC 3 cut(s) 1553, 2972, 3388
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 3 cut(s) 2871, 2907, 3401
Bpu10I CCTNAGC 1 cut(s) 3695
Bpu1102I GCTNAGC 1 cut(s) 2535
BpuEI CTTGAG 2 cut(s) 1095, 1225
BpuMI CCSGG 1 cut(s) 710
Bsa29I ATCGAT 1 cut(s) 3282
BsaAI YACGTR 2 cut(s) 2645, 3359
BsaBI GATNNNNATC 1 cut(s) 1764
BsaI GGTCTC 3 cut(s) 431, 712, 2643
BsaJI CCNNGG 6 cut(s) 708, 747, 1204, 1390, 2699, 3457
BsaWI WCCGGW 2 cut(s) 866, 2354
Bse1I ACTGG 9 cut(s) 185, 442, 970, 2223, 2346, 2770, 2888, 2924, 3230
Bse21I CCTNAGG 1 cut(s) 996
Bse3DI GCAATG 3 cut(s) 381, 2040, 2160
Bse8I GATNNNNATC 1 cut(s) 1764
BseAI TCCGGA 1 cut(s) 2354
BseBI CCWGG 6 cut(s) 723, 1119, 2700, 2982, 3420, 3464
BseCI ATCGAT 1 cut(s) 3282
BseDI CCNNGG 6 cut(s) 708, 747, 1204, 1390, 2699, 3457
BseJI GATNNNNATC 1 cut(s) 1764
BseMI GCAATG 3 cut(s) 381, 2040, 2160
BseMII CTCAG 7 cut(s) 104, 707, 987, 2549, 3609, 3638, 3686
BseNI ACTGG 9 cut(s) 185, 442, 970, 2223, 2346, 2770, 2888, 2924, 3230
BseRI GAGGAG 5 cut(s) 449, 503, 593, 1457, 2812
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 6 cut(s) 670, 770, 797, 1712, 2575, 3205
BsgI GTGCAG 1 cut(s) 777
Bsh1285I CGRYCG 1 cut(s) 648
BshFI GGCC 7 cut(s) 773, 1203, 1255, 2500, 2980, 3122, 3462
BshNI GGYRCC 3 cut(s) 809, 1931, 2902
BshVI ATCGAT 1 cut(s) 3282
BsiEI CGRYCG 1 cut(s) 648
BsiHKCI CYCGRG 1 cut(s) 1896
BsiSI CCGG 3 cut(s) 710, 867, 2355
BslFI GGGAC 3 cut(s) 350, 1235, 2328
BsmAI GTCTC 6 cut(s) 38, 150, 431, 712, 909, 2643
BsmBI CGTCTC 1 cut(s) 909
BsmFI GGGAC 3 cut(s) 350, 1235, 2328
BsmI GAATGC 3 cut(s) 1663, 1987, 2326
BsnI GGCC 7 cut(s) 773, 1203, 1255, 2500, 2980, 3122, 3462
Bso31I GGTCTC 3 cut(s) 431, 712, 2643
BsoBI CYCGRG 1 cut(s) 1896
Bsp1286I GDGCHC 3 cut(s) 81, 672, 812
Bsp13I TCCGGA 1 cut(s) 2354
Bsp1407I TGTACA 1 cut(s) 2639
Bsp1720I GCTNAGC 1 cut(s) 2535
Bsp19I CCATGG 2 cut(s) 1204, 1390
BspACI CCGC 5 cut(s) 140, 360, 592, 1038, 1354
BspANI GGCC 7 cut(s) 773, 1203, 1255, 2500, 2980, 3122, 3462
BspCNI CTCAG 7 cut(s) 103, 708, 988, 2548, 3608, 3637, 3687
BspDI ATCGAT 1 cut(s) 3282
BspEI TCCGGA 1 cut(s) 2354
BspLI GGNNCC 7 cut(s) 671, 706, 811, 1933, 2343, 2344, 2904
BspPI GGATC 5 cut(s) 534, 864, 2346, 2510, 2690
BspT107I GGYRCC 3 cut(s) 809, 1931, 2902
BspTI CTTAAG 1 cut(s) 3007
BspTNI GGTCTC 3 cut(s) 431, 712, 2643
BsrDI GCAATG 3 cut(s) 381, 2040, 2160
BsrGI TGTACA 1 cut(s) 2639
BsrI ACTGG 9 cut(s) 185, 442, 970, 2223, 2346, 2770, 2888, 2924, 3230
BssECI CCNNGG 6 cut(s) 708, 747, 1204, 1390, 2699, 3457
BssT1I CCWWGG 2 cut(s) 1204, 1390
Bst2UI CCWGG 6 cut(s) 723, 1119, 2700, 2982, 3420, 3464
Bst4CI ACNGT 9 cut(s) 163, 181, 485, 1608, 1995, 2181, 2259, 2897, 3586
Bst6I CTCTTC 5 cut(s) 894, 1410, 2232, 2718, 2766
BstAFI CTTAAG 1 cut(s) 3007
BstAUI TGTACA 1 cut(s) 2639
BstBAI YACGTR 2 cut(s) 2645, 3359
BstC8I GCNNGC 9 cut(s) 633, 790, 2567, 2861, 2907, 3341, 3345, 3364, 3368
BstDSI CCRYGG 3 cut(s) 1204, 1390, 3457
BstENI CCTNNNNNAGG 1 cut(s) 1800
BstH2I RGCGCY 1 cut(s) 2250
BstHHI GCGC 3 cut(s) 68, 1663, 2249
BstMAI GTCTC 6 cut(s) 38, 150, 431, 712, 909, 2643
BstMCI CGRYCG 1 cut(s) 648
BstNI CCWGG 6 cut(s) 723, 1119, 2700, 2982, 3420, 3464
BstNSI RCATGY 3 cut(s) 2169, 2961, 3343
BstSCI CCNGG 7 cut(s) 708, 721, 1117, 2698, 2980, 3418, 3462
BstSFI CTRYAG 4 cut(s) 1710, 3138, 3468, 3582
BstSLI GKGCMC 1 cut(s) 812
BstV1I GCAGC 6 cut(s) 670, 770, 797, 1712, 2575, 3205
BstV2I GAAGAC 3 cut(s) 1553, 2972, 3388
BstX2I RGATCY 5 cut(s) 526, 1400, 2398, 2465, 3485
BstXI CCANNNNNNTGG 1 cut(s) 2390
BstYI RGATCY 5 cut(s) 526, 1400, 2398, 2465, 3485
Bsu15I ATCGAT 1 cut(s) 3282
Bsu36I CCTNAGG 1 cut(s) 996
BsuI GTATCC 4 cut(s) 490, 1633, 2431, 3301
BsuRI GGCC 7 cut(s) 773, 1203, 1255, 2500, 2980, 3122, 3462
BsuTUI ATCGAT 1 cut(s) 3282
BtgI CCRYGG 3 cut(s) 1204, 1390, 3457
BtgZI GCGATG 2 cut(s) 2436, 3632
BtsIMutI CAGTG 8 cut(s) 177, 477, 478, 490, 1597, 2000, 2230, 3237
Cac8I GCNNGC 9 cut(s) 633, 790, 2567, 2861, 2907, 3341, 3345, 3364, 3368
CaiI CAGNNNCTG 3 cut(s) 728, 1010, 3631
CfoI GCGC 3 cut(s) 68, 1663, 2249
Cfr13I GGNCC 4 cut(s) 1253, 1453, 2342, 2498
ClaI ATCGAT 1 cut(s) 3282
CsiI ACCWGGT 2 cut(s) 721, 1117
Csp6I GTAC 4 cut(s) 548, 2148, 2640, 3192
CspCI CAANNNNNGTGG 4 cut(s) 675, 710, 1245, 1280
CviQI GTAC 4 cut(s) 548, 2148, 2640, 3192
DraI TTTAAA 1 cut(s) 2688
EaeI YGGCCR 3 cut(s) 1201, 3120, 3460
Eam1104I CTCTTC 5 cut(s) 894, 1410, 2232, 2718, 2766
EarI CTCTTC 5 cut(s) 894, 1410, 2232, 2718, 2766
EciI GGCGGA 1 cut(s) 1343
Eco130I CCWWGG 2 cut(s) 1204, 1390
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 2 cut(s) 81, 672
Eco31I GGTCTC 3 cut(s) 431, 712, 2643
Eco32I GATATC 2 cut(s) 412, 2751
Eco47I GGWCC 2 cut(s) 1453, 2342
Eco47III AGCGCT 1 cut(s) 2248
Eco57I CTGAAG 6 cut(s) 525, 1052, 1725, 1821, 3018, 3653
Eco72I CACGTG 2 cut(s) 2645, 3359
Eco81I CCTNAGG 1 cut(s) 996
Eco88I CYCGRG 1 cut(s) 1896
EcoNI CCTNNNNNAGG 1 cut(s) 1800
EcoO109I RGGNCCY 2 cut(s) 1453, 2342
EcoRI GAATTC 1 cut(s) 2784
EcoRII CCWGG 6 cut(s) 721, 1117, 2698, 2980, 3418, 3462
EcoRV GATATC 2 cut(s) 412, 2751
EcoT14I CCWWGG 2 cut(s) 1204, 1390
EcoT22I ATGCAT 2 cut(s) 199, 2920
EcoT38I GRGCYC 2 cut(s) 81, 672
ErhI CCWWGG 2 cut(s) 1204, 1390
Esp3I CGTCTC 1 cut(s) 909
FalI AAGNNNNNCTT 4 cut(s) 1050, 1082, 2697, 2729
FaqI GGGAC 3 cut(s) 350, 1235, 2328
FauNDI CATATG 1 cut(s) 3689
FbaI TGATCA 1 cut(s) 2485
FblI GTMKAC 2 cut(s) 648, 1539
Fnu4HI GCNGC 7 cut(s) 592, 684, 759, 786, 1701, 2589, 3219
FriOI GRGCYC 2 cut(s) 81, 672
Fsp4HI GCNGC 7 cut(s) 592, 684, 759, 786, 1701, 2589, 3219
FspI TGCGCA 1 cut(s) 67
GlaI GCGC 3 cut(s) 67, 1662, 2248
GluI GCNGC 7 cut(s) 592, 684, 759, 786, 1701, 2589, 3219
GsuI CTGGAG 3 cut(s) 2871, 2907, 3401
HaeII RGCGCY 1 cut(s) 2250
HaeIII GGCC 7 cut(s) 773, 1203, 1255, 2500, 2980, 3122, 3462
HapII CCGG 3 cut(s) 710, 867, 2355
HhaI GCGC 3 cut(s) 68, 1663, 2249
Hin6I GCGC 3 cut(s) 66, 1661, 2247
HinP1I GCGC 3 cut(s) 66, 1661, 2247
HincII GTYRAC 4 cut(s) 649, 1105, 1540, 2893
HindII GTYRAC 4 cut(s) 649, 1105, 1540, 2893
HindIII AAGCTT 2 cut(s) 2327, 2446
HpaI GTTAAC 1 cut(s) 2893
HpaII CCGG 3 cut(s) 710, 867, 2355
Hpy166II GTNNAC 7 cut(s) 649, 1105, 1540, 2642, 2893, 3263, 3589
Hpy8I GTNNAC 7 cut(s) 649, 1105, 1540, 2642, 2893, 3263, 3589
Hpy99I CGWCG 1 cut(s) 1604
HpyCH4III ACNGT 9 cut(s) 163, 181, 485, 1608, 1995, 2181, 2259, 2897, 3586
HpyCH4IV ACGT 2 cut(s) 2644, 3358
HpySE526I ACGT 2 cut(s) 2644, 3358
HspAI GCGC 3 cut(s) 66, 1661, 2247
KflI GGGWCCC 1 cut(s) 2342
Kpn2I TCCGGA 1 cut(s) 2354
Ksp22I TGATCA 1 cut(s) 2485
KspAI GTTAAC 1 cut(s) 2893
LmnI GCTCC 4 cut(s) 628, 675, 710, 3578
Lsp1109I GCAGC 6 cut(s) 670, 770, 797, 1712, 2575, 3205
MabI ACCWGGT 2 cut(s) 721, 1117
MaeII ACGT 2 cut(s) 2644, 3358
MaeIII GTNAC 5 cut(s) 1128, 3002, 3235, 3619, 3724
MfeI CAATTG 1 cut(s) 1744
MflI RGATCY 5 cut(s) 526, 1400, 2398, 2465, 3485
MhlI GDGCHC 3 cut(s) 81, 672, 812
MlsI TGGCCA 3 cut(s) 1203, 3122, 3462
MluNI TGGCCA 3 cut(s) 1203, 3122, 3462
MlyI GAGTC 6 cut(s) 500, 1100, 1193, 1613, 1801, 2249
MmeI TCCRAC 5 cut(s) 474, 1617, 2344, 2530, 2761
Mox20I TGGCCA 3 cut(s) 1203, 3122, 3462
Mph1103I ATGCAT 2 cut(s) 199, 2920
MroI TCCGGA 1 cut(s) 2354
MroXI GAANNNNTTC 2 cut(s) 1837, 3326
MscI TGGCCA 3 cut(s) 1203, 3122, 3462
MslI CAYNNNNRTG 6 cut(s) 38, 344, 1560, 2736, 3084, 3361
Msp20I TGGCCA 3 cut(s) 1203, 3122, 3462
MspA1I CMGCKG 2 cut(s) 1038, 1703
MspCI CTTAAG 1 cut(s) 3007
MspI CCGG 3 cut(s) 710, 867, 2355
MspR9I CCNGG 7 cut(s) 710, 723, 1119, 2700, 2982, 3420, 3464
MunI CAATTG 1 cut(s) 1744
Mva1269I GAATGC 3 cut(s) 1663, 1987, 2326
MvaI CCWGG 6 cut(s) 723, 1119, 2700, 2982, 3420, 3464
NciI CCSGG 1 cut(s) 710
NcoI CCATGG 2 cut(s) 1204, 1390
NdeI CATATG 1 cut(s) 3689
NlaIV GGNNCC 7 cut(s) 671, 706, 811, 1933, 2343, 2344, 2904
NmuCI GTSAC 3 cut(s) 1128, 3002, 3724
NsbI TGCGCA 1 cut(s) 67
NsiI ATGCAT 2 cut(s) 199, 2920
NspI RCATGY 3 cut(s) 2169, 2961, 3343
OliI CACNNNNGTG 2 cut(s) 344, 3361
PaeI GCATGC 1 cut(s) 3343
PaeR7I CTCGAG 1 cut(s) 1896
PceI AGGCCT 1 cut(s) 773
PcsI WCGNNNNNNNCGW 1 cut(s) 1903
PctI GAATGC 3 cut(s) 1663, 1987, 2326
PdmI GAANNNNTTC 2 cut(s) 1837, 3326
PfeI GAWTC 7 cut(s) 542, 1001, 1508, 1582, 2758, 2840, 2870
PflFI GACNNNGTC 1 cut(s) 920
PflMI CCANNNNNTGG 6 cut(s) 268, 295, 322, 2659, 3082, 3690
PkrI GCNGC 7 cut(s) 593, 685, 760, 787, 1702, 2590, 3220
PleI GAGTC 6 cut(s) 499, 1100, 1192, 1613, 1800, 2248
PmaCI CACGTG 2 cut(s) 2645, 3359
PmlI CACGTG 2 cut(s) 2645, 3359
PpsI GAGTC 6 cut(s) 499, 1100, 1192, 1613, 1800, 2248
Ppu21I YACGTR 2 cut(s) 2645, 3359
PpuMI RGGWCCY 2 cut(s) 1453, 2342
PshBI ATTAAT 2 cut(s) 239, 266
Psp5II RGGWCCY 2 cut(s) 1453, 2342
Psp6I CCWGG 6 cut(s) 721, 1117, 2698, 2980, 3418, 3462
PspCI CACGTG 2 cut(s) 2645, 3359
PspGI CCWGG 6 cut(s) 721, 1117, 2698, 2980, 3418, 3462
PspN4I GGNNCC 7 cut(s) 671, 706, 811, 1933, 2343, 2344, 2904
PspPI GGNCC 4 cut(s) 1253, 1453, 2342, 2498
PspPPI RGGWCCY 2 cut(s) 1453, 2342
PstNI CAGNNNCTG 3 cut(s) 728, 1010, 3631
PsuI RGATCY 5 cut(s) 526, 1400, 2398, 2465, 3485
PsyI GACNNNGTC 1 cut(s) 920
PvuII CAGCTG 1 cut(s) 1703
RsaI GTAC 4 cut(s) 549, 2149, 2641, 3193
RsaNI GTAC 4 cut(s) 548, 2148, 2640, 3192
RseI CAYNNNNRTG 6 cut(s) 38, 344, 1560, 2736, 3084, 3361
SalI GTCGAC 2 cut(s) 647, 1538
SatI GCNGC 7 cut(s) 592, 684, 759, 786, 1701, 2589, 3219
Sau96I GGNCC 4 cut(s) 1253, 1453, 2342, 2498
SchI GAGTC 6 cut(s) 500, 1100, 1193, 1613, 1801, 2249
ScrFI CCNGG 7 cut(s) 710, 723, 1119, 2700, 2982, 3420, 3464
SduI GDGCHC 3 cut(s) 81, 672, 812
SexAI ACCWGGT 2 cut(s) 721, 1117
SfcI CTRYAG 4 cut(s) 1710, 3138, 3468, 3582
Sfr274I CTCGAG 1 cut(s) 1896
SinI GGWCC 2 cut(s) 1453, 2342
SlaI CTCGAG 1 cut(s) 1896
SmiMI CAYNNNNRTG 6 cut(s) 38, 344, 1560, 2736, 3084, 3361
SmlI CTYRAG 4 cut(s) 1110, 1240, 1896, 3007
SmoI CTYRAG 4 cut(s) 1110, 1240, 1896, 3007
SphI GCATGC 1 cut(s) 3343
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 5 cut(s) 140, 360, 592, 1038, 1354
SspI AATATT 4 cut(s) 586, 1263, 2283, 2945
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 7 cut(s) 708, 721, 1117, 2698, 2980, 3418, 3462
StyI CCWWGG 2 cut(s) 1204, 1390
TaaI ACNGT 9 cut(s) 163, 181, 485, 1608, 1995, 2181, 2259, 2897, 3586
TaiI ACGT 2 cut(s) 2647, 3361
TaqII GACCGA 1 cut(s) 634
TatI WGTACW 1 cut(s) 2639
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 7 cut(s) 542, 1001, 1508, 1582, 2758, 2840, 2870
TscAI CASTG 8 cut(s) 184, 478, 484, 490, 1597, 2000, 2230, 3237
TseFI GTSAC 3 cut(s) 1128, 3002, 3724
TseI GCWGC 6 cut(s) 683, 758, 785, 1700, 2588, 3218
Tsp45I GTSAC 3 cut(s) 1128, 3002, 3724
TspGWI ACGGA 3 cut(s) 565, 2990, 3446
TspRI CASTG 8 cut(s) 184, 478, 484, 490, 1597, 2000, 2230, 3237
Tth111I GACNNNGTC 1 cut(s) 920
Van91I CCANNNNNTGG 6 cut(s) 268, 295, 322, 2659, 3082, 3690
Vha464I CTTAAG 1 cut(s) 3007
VpaK11BI GGWCC 2 cut(s) 1453, 2342
VspI ATTAAT 2 cut(s) 239, 266
XagI CCTNNNNNAGG 1 cut(s) 1800
XceI RCATGY 3 cut(s) 2169, 2961, 3343
XhoI CTCGAG 1 cut(s) 1896
XmiI GTMKAC 2 cut(s) 648, 1539
XmnI GAANNNNTTC 2 cut(s) 1837, 3326
Zsp2I ATGCAT 2 cut(s) 199, 2920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.