MD16G1176700.v1.1

Regulator of Ty1 transposition protein 107 BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007475
Physical Location & Seq
Forward (+)
15037950 .. 15044231
6282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1176700.v1.1.491

Sequence Viewer

Length: 3786 bp
ATGGGTTCCCTCTGGGACGGCCACGACGAAATCAAGCCGATGGAGAAAAACTCACTCGCCGACTGCGCCGATACCGAGCCCATTGATACTCAGATTTCAAGCCCTTCATTGTCCGATGAGAAGGGCATATCGATGGATGCGGATGAGTTAGTGCGAGACATTGTGCCGTCTGATGATACTGTTCCAGTTGAAGATGCATTTGAAACCCAAATGGTAGATTTTGGTGATGAAACCCAATTAATGGATTTCGCAACCGAAACCCAAGTAATGGATTTTGGTGGCGAAACCGAAGTATTGGATTTTGGTGGCGAAACCCAAGTATTGGATTTTGGTGGCGAAACCCAAGTATTGGATTTGGGCGGGGAAACACAAGCGATGGATTTCGGTGGTGAAACACAAGCAGTGGATTTCAGTGGTGAAACACAAGTGTTGGATGATATCAACTGCTTTGCGAACATGGAGGATACTCAGTTATTAGAGTTTGATGATGTAGTTGTCAGTGACAGTGAAGAGTCGGATGCAACTGAAGTTTTGGATGATAGTAAGGATCTAACTGACAATGAATCGGTACAGAGAGGTTCTGGTCAATTAATGAATGAGGAGAATATTTGCCGCACTCCTTGTGAAAATAGTGAAAATGGGTTAACGGAGCAAGCCAATCATTTGATCGACAAGCAACACAATGCAGGGCTCCATGTTTCTGCTGCAACACCTGTGGATGACGGCTCCCCGGAACTGGGACCAGGTTCTGTGCATATGCAATTTACCTCAGTCCATGCAGCATCCTTACAGGCCTCCGGTCTAGCAGCTCACAGTACAGCTTTGAATGGTACCGACAGTGAGTCTCGTTCTGTTCCAAGTAATAACCAATCTTTGAATCAACTTTCCGGGAAAGATAATGCAGTATCTCTTTTAGGGGGTTCGACCATAGATGGGGAAGAAGTCAACGAGGAACATGATTCAAGGAATGAGAACAAATGGAGGACTGGTAGTTCAACAGCGAGAAAACTTTTTACTGAGTATTCAGATGCTGAAAACACGGAAATTTCTCATAACAGCGCTAGTGATGAAGAAGCTGAAGATTTGCTTCAGTTTCCTAGTAATTTGGCAGGGTTGAGCTATATTGACTCTCAAGAACCAGGTGAATTATCACAGGCTAATGCACTTGATTTTGTGGATAAATTCCTCCAAAATAATCTTGATGAGTCTAATAAAGAAGTTGGCCATGGAAAGAGTGCAAGGGACAATTCAAGATTTGTCTCAAGTGCAAAAGGGCCACAAACTTTGGCCAAGAAAGCCAATGACAAAAGCATAGATAAAGGGATTTTCGATTGGGATGATAGCCGCGAGGATGAAGAAGGAGGTGAATTTTTCCGCCGAAGAAAAGCAGACTTCTTTGATGGCAGAAGCCATGGGTGGAGATCTCTTCCCCAACCCCAGAAGTCCAAAGAAAAAAGACAGGATGCGGAGAAGGATCGCAAAAAACAATTACAAGGAAAGAACAAGAGAATAAGTGTAGTTCATTCTGATTCAAAACTATTGTTGCATAATTCAAAAGTCGACAAGAAGACAGCACATGAAGATGAAATGAAACATAAAAAGAATCTCGTCAGTGAGTTTGATGAACAGTTTAACAATGACTCCCCTAGAGAGAAGTTGGATGCTAATATTAATAAGAACGATGCACCTGAAATGATGAATGTAGGCTTTGATACTCAAATGGCAGCTGAAGCTATAGAAGCCTTGTGTTATGGCGAAGGGATTTCCAATTGTGATGCTAGTGATGACTATCAAGATGCTCAAGGAAATCAGAGTTCTCCTGAAAGTTCGATGGGAGAAAAATCAAAGAATAGAACTTGTTCAACGAAACAGTCTTCTCGAAAAAGAGGGCGTTTTACTGATGCAGGGGTTTCCCGAGAAACTCAACAAGCAAAGAAAATGAGGGTTGGTGGCAGATCAAGTAAACATTCTTCAGTTTCACCTCTGGAATATTCTAAGAATACCAGGAAGGAGTGTGAAACAAAGGTGGTGATGGCAAAATCAAAGAAGGGGAAGTCCAATGCTATAAAGCTTCTTAACATCATTGGGAACAGAAACATGGAGAAAATGCCTTCTGTTGCTATTGACCTAAGAACAGAAGGATCTATAAAAAAGCATTTACGACAGGATGTTGGTACGTTTACGCCGATTGCACGTCGAACTAGACGGTCTATGGTGGTAAATCAGTTAAAGAAAGCTGACGATGCATCCAGTGATTGTGGAGAAGAGTCAAGCTCTCAAACAGAGGATGTTGCAACTAGAGAAAAAATAATTAGTTTTACAGGTGTTCCGGTGGCTAATGCGTTGAATGCAAAATCTTCAAAATCGGGTCCCAATCGATCCGGAGAAGTTGGAAATCATAAACCAAGCCAACATCATGGTTCAGATCTCAAGTTTGAAGCTATTTGCAATGGCATCAAATTGGATGCTTTAAGCTTCCCCAAAGGAAAAAGATCTAGGAGAAAGTTATCTGATCAGGTTTATGGGCCTGATAACTTAAATGATCCACCGACCCCATCTGTTCAGCCAGACAAAGTTGGACAACGTGTCACTAGGCACACAAGACCGCAGGGTGCTGCTCAAAGCATTTTTGTTGATGTAAAGTCAACAAGAAGAACCCGATCGGCTACACGTGGTGACAAGAATTGTGCTAGAAAATTTGCACGTCGAAGTTTAAAAACTGATCCTTGGAAAGCTCCTCTTCATTGTAATTCTTCTCATAAGGATGGGATAATGATATCAGAGATTACAACTGGTGGAGAGGCAGTTGGAATTCTTGACAGGGCGAGTGATGCAAATCCGTCTTCTGCTACAAAAATGAGGGATGAATCCCCGCTAGGGAAATGCAAACCACTGGATTCAGCTTGTGCTACTCCAGTTAACAGTAAGGTGCCTGTCAATGATGCATCTCCAGTTTGTATGGGTAATGAATATTTCAAACAGTCATGCAAGAATACCCCTTCAAGGCCAAGTCTTTTGAAAGAAATCCGTGACTTAAGTGCAAATGGGCATACACCAACTTCAGCATCAAAGGATTTAAGGAAGAGGAGAGATATGACAGAAGTTCGAGTATTGTATAGCCACCACTTGGATGACTATGTAATTAAGCATCAGAAAAAGATTTTGGCCCGACTAGGAGTTTCTGTAGCATCCTCTATGACAGATGCCACACACTTCATAGCAGATCAATTTGTGCGTACGAGGAATATGTTAGAAGCTATTGCTGCTGGAAAACCAGTGGTGACGCATTTATGGCTCGATAGCTGTGGACAAGCTAGTTGTTTCATCGATGAGAAAAACTATATACTAAGGGATACCAAGAAGGAGAAGGAGTTTGGCTTTAACATGCCGACTTCGTTGGTACGTGCGTGCCAGCATCCACTTCTGGAGGGTCGTAAAGTCTTCATCACCCCAACTACGAAGCCTGGTAAAGAAATAATTTCAAGTCTGGTCAAAGCAGTCCATGGCCAGGCTATAGAAAGAATTGGTAGATCTGTTTTGGAGGCTGATAAAATTCCAGATGATCTGTTGGTTCTATCTTGTGAAGAAGATTATGAAATTTGTGTGCCTATACTCGAAAAAGGAGCTACAGTTTACAGCTCAGAGTTGCTATTGAATGGGATTGTTACTCAGAAGCTGGAGTTTGAAAGGCATTGCCTCTTCACGGATCAAGTTAAGAAAACCCGTTCGACCATATGGCTAAGGAAAGATGGCAGTAAGTTCCTCCCTGTGACAAAAAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000414 GO:0000416 GO:0000726 GO:0002637 GO:0002639 GO:0002682 GO:0002684 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002703 GO:0002705 GO:0002706 GO:0002708 GO:0002712 GO:0002714 GO:0002819 GO:0002821 GO:0002822 GO:0002824 GO:0002889 GO:0002891 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006303 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010212 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030330 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034708 GO:0034968 GO:0035065 GO:0035066 GO:0035097 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043414 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044666 GO:0045191 GO:0045830 GO:0045893 GO:0045911 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0050867 GO:0050871 GO:0050896 GO:0051052 GO:0051054 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051249 GO:0051251 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0051569 GO:0051571 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072331 GO:0080090 GO:0090304 GO:1901360 GO:1901564 GO:1901983 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903320 GO:1903322 GO:1903506 GO:1903508 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000142 GO:2000144 GO:2000756 GO:2000758 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RTT107_BRCT_5

Protein Analysis

1262

Amino Acids

138.91

Weight (kDa)

5.56

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTT107_BRCT_5 PF16770 1023 - 1116 1.1e-09 Regulator of Ty1 transposition protein 107 BRCT domain
BRCT_2 PF16589 1140 - 1227 6.1e-17 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 830
AccB1I GGYRCC 2 cut(s) 830, 2929
AccB7I CCANNNNNTGG 5 cut(s) 241, 268, 322, 349, 3127
AccI GTMKAC 1 cut(s) 1560
AccII CGCG 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 2381
AciI CCGC 8 cut(s) 140, 360, 613, 1345, 1375, 1466, 2606, 2873
AclWI GGATC 7 cut(s) 555, 1482, 2149, 2373, 2537, 2717, 3714
AcoI YGGCCR 4 cut(s) 19, 1222, 1287, 3505
AcsI RAATTY 7 cut(s) 1044, 1181, 1367, 2696, 2811, 3552, 3597
AcuI CTGAAG 6 cut(s) 546, 1073, 1098, 1749, 1956, 3045
AcvI CACGTG 1 cut(s) 2672
AdeI CACNNNGTG 1 cut(s) 2675
AfaI GTAC 6 cut(s) 570, 817, 832, 2176, 3238, 3402
AfeI AGCGCT 1 cut(s) 1060
AflII CTTAAG 1 cut(s) 3034
AflIII ACRYGT 2 cut(s) 2584, 2669
AhdI GACNNNNNGTC 2 cut(s) 841, 2585
AjiI CACGTC 2 cut(s) 2195, 2705
AjnI CCWGG 5 cut(s) 742, 1138, 2003, 3463, 3507
AjuI GAANNNNNNNTTGG 2 cut(s) 3146, 3178
AloI GAACNNNNNNTCC 2 cut(s) 976, 1008
Alw26I GTCTC 3 cut(s) 150, 849, 1264
AlwI GGATC 7 cut(s) 555, 1482, 2149, 2373, 2537, 2717, 3714
AlwNI CAGNNNCTG 3 cut(s) 749, 1031, 3676
Ama87I CYCGRG 1 cut(s) 1914
Aor13HI TCCGGA 1 cut(s) 2381
Aor51HI AGCGCT 1 cut(s) 1060
AoxI GGCC 9 cut(s) 19, 792, 1222, 1274, 1287, 2525, 3005, 3165, 3505
ApeKI GCWGC 6 cut(s) 704, 779, 806, 1724, 2615, 3263
ApoI RAATTY 7 cut(s) 1044, 1181, 1367, 2696, 2811, 3552, 3597
AseI ATTAAT 3 cut(s) 239, 590, 1671
Asp700I GAANNNNTTC 1 cut(s) 1858
Asp718I GGTACC 1 cut(s) 830
AspLEI GCGC 2 cut(s) 68, 1061
AspS9I GGNCC 5 cut(s) 740, 1274, 2369, 2525, 3166
AsuC2I CCSGG 2 cut(s) 731, 889
AvaI CYCGRG 1 cut(s) 1914
AvaII GGWCC 2 cut(s) 740, 2369
BaeI ACNNNNGTAYC 2 cut(s) 3392, 3425
BalI TGGCCA 3 cut(s) 1224, 1289, 3507
BanI GGYRCC 2 cut(s) 830, 2929
BanII GRGCYC 2 cut(s) 81, 693
BarI GAAGNNNNNNTAC 2 cut(s) 1954, 1986
BbrPI CACGTG 1 cut(s) 2672
BbsI GAAGAC 4 cut(s) 1574, 1866, 2835, 3433
BbvI GCAGC 6 cut(s) 691, 791, 818, 1736, 2602, 3250
BceAI ACGGC 3 cut(s) 34, 151, 739
BciT130I CCWGG 5 cut(s) 744, 1140, 2005, 3465, 3509
BciVI GTATCC 2 cut(s) 457, 3346
BclI TGATCA 1 cut(s) 2512
BcnI CCSGG 2 cut(s) 731, 889
BcoDI GTCTC 3 cut(s) 150, 849, 1264
BfmI CTRYAG 4 cut(s) 1734, 3183, 3513, 3627
BfoI RGCGCY 1 cut(s) 1062
BfrI CTTAAG 1 cut(s) 3034
BfuI GTATCC 2 cut(s) 457, 3346
BglII AGATCT 4 cut(s) 1421, 2425, 2492, 3530
BisI GCNGC 8 cut(s) 613, 705, 780, 807, 1345, 1725, 2616, 3264
BlsI GCNGC 8 cut(s) 614, 706, 781, 808, 1346, 1726, 2617, 3265
Bme1390I CCNGG 7 cut(s) 731, 744, 889, 1140, 2005, 3465, 3509
Bme18I GGWCC 2 cut(s) 740, 2369
BmeRI GACNNNNNGTC 2 cut(s) 841, 2585
BmeT110I CYCGRG 1 cut(s) 1914
BmgBI CACGTC 2 cut(s) 2195, 2705
BmgT120I GGNCC 5 cut(s) 740, 1274, 2369, 2525, 3166
BmiI GGNNCC 8 cut(s) 7, 692, 727, 741, 832, 2370, 2371, 2931
BmrFI CCNGG 7 cut(s) 731, 744, 889, 1140, 2005, 3465, 3509
BmrI ACTGGG 1 cut(s) 746
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 4 cut(s) 1574, 1866, 2835, 3433
BplI GAGNNNNNCTC 4 cut(s) 35, 67, 2258, 2290
BpmI CTGGAG 4 cut(s) 2898, 2934, 3446, 3698
Bpu10I CCTNAGC 1 cut(s) 3740
BpuEI CTTGAG 4 cut(s) 1116, 1246, 1785, 2414
BpuMI CCSGG 2 cut(s) 731, 889
Bsa29I ATCGAT 3 cut(s) 131, 2377, 3327
BsaAI YACGTR 2 cut(s) 2672, 3404
BsaBI GATNNNNATC 2 cut(s) 1788, 2835
BsaJI CCNNGG 5 cut(s) 729, 1225, 1411, 2726, 3502
BsaWI WCCGGW 3 cut(s) 797, 2329, 2381
Bse1I ACTGG 9 cut(s) 185, 741, 991, 2250, 2797, 2898, 2915, 2951, 3275
Bse3DI GCAATG 2 cut(s) 2455, 3691
Bse8I GATNNNNATC 2 cut(s) 1788, 2835
BseAI TCCGGA 1 cut(s) 2381
BseBI CCWGG 5 cut(s) 744, 1140, 2005, 3465, 3509
BseCI ATCGAT 3 cut(s) 131, 2377, 3327
BseDI CCNNGG 5 cut(s) 729, 1225, 1411, 2726, 3502
BseJI GATNNNNATC 2 cut(s) 1788, 2835
BseMI GCAATG 2 cut(s) 2455, 3691
BseMII CTCAG 6 cut(s) 104, 482, 783, 1008, 3654, 3683
BseNI ACTGG 9 cut(s) 185, 741, 991, 2250, 2797, 2898, 2915, 2951, 3275
BseRI GAGGAG 3 cut(s) 614, 2727, 3100
BseXI GCAGC 6 cut(s) 691, 791, 818, 1736, 2602, 3250
Bsh1236I CGCG 1 cut(s) 1347
Bsh1285I CGRYCG 1 cut(s) 2663
BshFI GGCC 9 cut(s) 21, 794, 1224, 1276, 1289, 2527, 3007, 3167, 3507
BshNI GGYRCC 2 cut(s) 830, 2929
BshVI ATCGAT 3 cut(s) 131, 2377, 3327
BsiEI CGRYCG 1 cut(s) 2663
BsiHKCI CYCGRG 1 cut(s) 1914
BsiSI CCGG 5 cut(s) 731, 798, 888, 2330, 2382
BsiWI CGTACG 1 cut(s) 3236
BslFI GGGAC 4 cut(s) 29, 753, 1256, 2355
BsmAI GTCTC 3 cut(s) 150, 849, 1264
BsmFI GGGAC 4 cut(s) 29, 753, 1256, 2355
BsmI GAATGC 1 cut(s) 2353
BsnI GGCC 9 cut(s) 21, 794, 1224, 1276, 1289, 2527, 3007, 3167, 3507
BsoBI CYCGRG 1 cut(s) 1914
Bsp1286I GDGCHC 2 cut(s) 81, 693
Bsp13I TCCGGA 1 cut(s) 2381
Bsp19I CCATGG 3 cut(s) 1225, 1411, 3502
BspACI CCGC 8 cut(s) 140, 360, 613, 1345, 1375, 1466, 2606, 2873
BspANI GGCC 9 cut(s) 21, 794, 1224, 1276, 1289, 2527, 3007, 3167, 3507
BspCNI CTCAG 6 cut(s) 103, 481, 782, 1009, 3653, 3682
BspDI ATCGAT 3 cut(s) 131, 2377, 3327
BspEI TCCGGA 1 cut(s) 2381
BspFNI CGCG 1 cut(s) 1347
BspLI GGNNCC 8 cut(s) 7, 692, 727, 741, 832, 2370, 2371, 2931
BspPI GGATC 7 cut(s) 555, 1482, 2149, 2373, 2537, 2717, 3714
BspT107I GGYRCC 2 cut(s) 830, 2929
BspTI CTTAAG 1 cut(s) 3034
BsrDI GCAATG 2 cut(s) 2455, 3691
BsrI ACTGG 9 cut(s) 185, 741, 991, 2250, 2797, 2898, 2915, 2951, 3275
BssECI CCNNGG 5 cut(s) 729, 1225, 1411, 2726, 3502
BssT1I CCWWGG 4 cut(s) 1225, 1411, 2726, 3502
Bst2UI CCWGG 5 cut(s) 744, 1140, 2005, 3465, 3509
Bst6I CTCTTC 6 cut(s) 504, 1431, 2259, 2745, 3077, 3704
BstAFI CTTAAG 1 cut(s) 3034
BstBAI YACGTR 2 cut(s) 2672, 3404
BstC8I GCNNGC 3 cut(s) 654, 3409, 3413
BstDSI CCRYGG 3 cut(s) 1225, 1411, 3502
BstFNI CGCG 1 cut(s) 1347
BstH2I RGCGCY 1 cut(s) 1062
BstHHI GCGC 2 cut(s) 68, 1061
BstMAI GTCTC 3 cut(s) 150, 849, 1264
BstMCI CGRYCG 1 cut(s) 2663
BstMWI GCNNNNNNNGC 9 cut(s) 65, 1295, 1730, 1739, 2243, 2348, 2831, 3263, 3292
BstNI CCWGG 5 cut(s) 744, 1140, 2005, 3465, 3509
BstNSI RCATGY 1 cut(s) 3388
BstSCI CCNGG 7 cut(s) 729, 742, 887, 1138, 2003, 3463, 3507
BstSFI CTRYAG 4 cut(s) 1734, 3183, 3513, 3627
BstUI CGCG 1 cut(s) 1347
BstV1I GCAGC 6 cut(s) 691, 791, 818, 1736, 2602, 3250
BstV2I GAAGAC 4 cut(s) 1574, 1866, 2835, 3433
BstX2I RGATCY 6 cut(s) 547, 1421, 2141, 2425, 2492, 3530
BstXI CCANNNNNNTGG 1 cut(s) 2417
BstYI RGATCY 6 cut(s) 547, 1421, 2141, 2425, 2492, 3530
Bsu15I ATCGAT 3 cut(s) 131, 2377, 3327
BsuI GTATCC 2 cut(s) 457, 3346
BsuRI GGCC 9 cut(s) 21, 794, 1224, 1276, 1289, 2527, 3007, 3167, 3507
BsuTUI ATCGAT 3 cut(s) 131, 2377, 3327
BtgI CCRYGG 3 cut(s) 1225, 1411, 3502
BtgZI GCGATG 1 cut(s) 389
BtrI CACGTC 2 cut(s) 2195, 2705
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 9 cut(s) 408, 418, 505, 511, 844, 1618, 2257, 2891, 3282
Cac8I GCNNGC 3 cut(s) 654, 3409, 3413
CaiI CAGNNNCTG 3 cut(s) 749, 1031, 3676
CfoI GCGC 2 cut(s) 68, 1061
Cfr13I GGNCC 5 cut(s) 740, 1274, 2369, 2525, 3166
ClaI ATCGAT 3 cut(s) 131, 2377, 3327
CseI GACGC 1 cut(s) 3292
CsiI ACCWGGT 2 cut(s) 742, 1138
Csp6I GTAC 6 cut(s) 569, 816, 831, 2175, 3237, 3401
CspCI CAANNNNNGTGG 4 cut(s) 696, 731, 1266, 1301
CviQI GTAC 6 cut(s) 569, 816, 831, 2175, 3237, 3401
DraI TTTAAA 1 cut(s) 2715
DraIII CACNNNGTG 1 cut(s) 2675
DriI GACNNNNNGTC 2 cut(s) 841, 2585
EaeI YGGCCR 4 cut(s) 19, 1222, 1287, 3505
Eam1104I CTCTTC 6 cut(s) 504, 1431, 2259, 2745, 3077, 3704
Eam1105I GACNNNNNGTC 2 cut(s) 841, 2585
EarI CTCTTC 6 cut(s) 504, 1431, 2259, 2745, 3077, 3704
EciI GGCGGA 1 cut(s) 1364
Eco130I CCWWGG 4 cut(s) 1225, 1411, 2726, 3502
Eco147I AGGCCT 1 cut(s) 794
Eco24I GRGCYC 2 cut(s) 81, 693
Eco32I GATATC 2 cut(s) 439, 2778
Eco47I GGWCC 2 cut(s) 740, 2369
Eco47III AGCGCT 1 cut(s) 1060
Eco57I CTGAAG 6 cut(s) 546, 1073, 1098, 1749, 1956, 3045
Eco72I CACGTG 1 cut(s) 2672
Eco88I CYCGRG 1 cut(s) 1914
EcoO109I RGGNCCY 1 cut(s) 2369
EcoRI GAATTC 1 cut(s) 2811
EcoRII CCWGG 5 cut(s) 742, 1138, 2003, 3463, 3507
EcoRV GATATC 2 cut(s) 439, 2778
EcoT14I CCWWGG 4 cut(s) 1225, 1411, 2726, 3502
EcoT22I ATGCAT 3 cut(s) 199, 2248, 2947
EcoT38I GRGCYC 2 cut(s) 81, 693
ErhI CCWWGG 4 cut(s) 1225, 1411, 2726, 3502
FalI AAGNNNNNCTT 4 cut(s) 1071, 1103, 2724, 2756
FaqI GGGAC 4 cut(s) 29, 753, 1256, 2355
FauI CCCGC 2 cut(s) 353, 2880
FauNDI CATATG 2 cut(s) 756, 3734
FbaI TGATCA 1 cut(s) 2512
FblI GTMKAC 1 cut(s) 1560
Fnu4HI GCNGC 8 cut(s) 613, 705, 780, 807, 1345, 1725, 2616, 3264
FriOI GRGCYC 2 cut(s) 81, 693
Fsp4HI GCNGC 8 cut(s) 613, 705, 780, 807, 1345, 1725, 2616, 3264
GlaI GCGC 2 cut(s) 67, 1060
GluI GCNGC 8 cut(s) 613, 705, 780, 807, 1345, 1725, 2616, 3264
GsuI CTGGAG 4 cut(s) 2898, 2934, 3446, 3698
HaeII RGCGCY 1 cut(s) 1062
HaeIII GGCC 9 cut(s) 21, 794, 1224, 1276, 1289, 2527, 3007, 3167, 3507
HapII CCGG 5 cut(s) 731, 798, 888, 2330, 2382
HgaI GACGC 1 cut(s) 3292
HhaI GCGC 2 cut(s) 68, 1061
Hin6I GCGC 2 cut(s) 66, 1059
HinP1I GCGC 2 cut(s) 66, 1059
HincII GTYRAC 5 cut(s) 645, 946, 1561, 2646, 2920
HindII GTYRAC 5 cut(s) 645, 946, 1561, 2646, 2920
HindIII AAGCTT 2 cut(s) 2069, 2473
HpaI GTTAAC 2 cut(s) 645, 2920
HpaII CCGG 5 cut(s) 731, 798, 888, 2330, 2382
Hpy166II GTNNAC 9 cut(s) 645, 946, 1561, 1964, 2181, 2646, 2920, 3308, 3634
Hpy8I GTNNAC 9 cut(s) 645, 946, 1561, 1964, 2181, 2646, 2920, 3308, 3634
Hpy99I CGWCG 3 cut(s) 29, 2199, 2709
HpyCH4IV ACGT 6 cut(s) 2177, 2194, 2584, 2671, 2704, 3403
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 1295, 1730, 1739, 2243, 2348, 2831, 3263, 3292
HpySE526I ACGT 6 cut(s) 2177, 2194, 2584, 2671, 2704, 3403
HspAI GCGC 2 cut(s) 66, 1059
KflI GGGWCCC 1 cut(s) 2369
Kpn2I TCCGGA 1 cut(s) 2381
KpnI GGTACC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 2512
KspAI GTTAAC 2 cut(s) 645, 2920
LmnI GCTCC 5 cut(s) 649, 696, 731, 2740, 3623
Lsp1109I GCAGC 6 cut(s) 691, 791, 818, 1736, 2602, 3250
MabI ACCWGGT 2 cut(s) 742, 1138
MaeII ACGT 6 cut(s) 2177, 2194, 2584, 2671, 2704, 3403
MaeIII GTNAC 7 cut(s) 500, 2587, 2675, 3029, 3280, 3664, 3769
MfeI CAATTG 1 cut(s) 1768
MflI RGATCY 6 cut(s) 547, 1421, 2141, 2425, 2492, 3530
MhlI GDGCHC 2 cut(s) 81, 693
MlsI TGGCCA 3 cut(s) 1224, 1289, 3507
MluNI TGGCCA 3 cut(s) 1224, 1289, 3507
MlyI GAGTC 6 cut(s) 521, 851, 1121, 1214, 1634, 2276
MmeI TCCRAC 6 cut(s) 411, 495, 1638, 2371, 2557, 2788
Mox20I TGGCCA 3 cut(s) 1224, 1289, 3507
Mph1103I ATGCAT 3 cut(s) 199, 2248, 2947
MroI TCCGGA 1 cut(s) 2381
MroXI GAANNNNTTC 1 cut(s) 1858
MscI TGGCCA 3 cut(s) 1224, 1289, 3507
MslI CAYNNNNRTG 4 cut(s) 131, 1581, 2763, 3129
Msp20I TGGCCA 3 cut(s) 1224, 1289, 3507
MspA1I CMGCKG 1 cut(s) 1727
MspCI CTTAAG 1 cut(s) 3034
MspI CCGG 5 cut(s) 731, 798, 888, 2330, 2382
MspR9I CCNGG 7 cut(s) 731, 744, 889, 1140, 2005, 3465, 3509
MunI CAATTG 1 cut(s) 1768
Mva1269I GAATGC 1 cut(s) 2353
MvaI CCWGG 5 cut(s) 744, 1140, 2005, 3465, 3509
MvnI CGCG 1 cut(s) 1347
MwoI GCNNNNNNNGC 9 cut(s) 65, 1295, 1730, 1739, 2243, 2348, 2831, 3263, 3292
NciI CCSGG 2 cut(s) 731, 889
NcoI CCATGG 3 cut(s) 1225, 1411, 3502
NdeI CATATG 2 cut(s) 756, 3734
NlaIV GGNNCC 8 cut(s) 7, 692, 727, 741, 832, 2370, 2371, 2931
NmuCI GTSAC 6 cut(s) 500, 2587, 2675, 3029, 3280, 3769
NsiI ATGCAT 3 cut(s) 199, 2248, 2947
NspI RCATGY 1 cut(s) 3388
PceI AGGCCT 1 cut(s) 794
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 2183
PctI GAATGC 1 cut(s) 2353
PdmI GAANNNNTTC 1 cut(s) 1858
PfeI GAWTC 7 cut(s) 563, 877, 959, 1529, 1603, 2867, 2897
Pfl23II CGTACG 1 cut(s) 3236
PflMI CCANNNNNTGG 5 cut(s) 241, 268, 322, 349, 3127
PfoI TCCNGGA 1 cut(s) 887
PkrI GCNGC 8 cut(s) 614, 706, 781, 808, 1346, 1726, 2617, 3265
Ple19I CGATCG 1 cut(s) 2663
PleI GAGTC 6 cut(s) 520, 850, 1121, 1213, 1634, 2275
PmaCI CACGTG 1 cut(s) 2672
PmlI CACGTG 1 cut(s) 2672
PpsI GAGTC 6 cut(s) 520, 850, 1121, 1213, 1634, 2275
Ppu21I YACGTR 2 cut(s) 2672, 3404
PpuMI RGGWCCY 1 cut(s) 2369
PshBI ATTAAT 3 cut(s) 239, 590, 1671
Psp5II RGGWCCY 1 cut(s) 2369
Psp6I CCWGG 5 cut(s) 742, 1138, 2003, 3463, 3507
PspCI CACGTG 1 cut(s) 2672
PspGI CCWGG 5 cut(s) 742, 1138, 2003, 3463, 3507
PspLI CGTACG 1 cut(s) 3236
PspN4I GGNNCC 8 cut(s) 7, 692, 727, 741, 832, 2370, 2371, 2931
PspPI GGNCC 5 cut(s) 740, 1274, 2369, 2525, 3166
PspPPI RGGWCCY 1 cut(s) 2369
PstNI CAGNNNCTG 3 cut(s) 749, 1031, 3676
PsuI RGATCY 6 cut(s) 547, 1421, 2141, 2425, 2492, 3530
PvuI CGATCG 1 cut(s) 2663
PvuII CAGCTG 1 cut(s) 1727
RsaI GTAC 6 cut(s) 570, 817, 832, 2176, 3238, 3402
RsaNI GTAC 6 cut(s) 569, 816, 831, 2175, 3237, 3401
RseI CAYNNNNRTG 4 cut(s) 131, 1581, 2763, 3129
SalI GTCGAC 1 cut(s) 1559
SatI GCNGC 8 cut(s) 613, 705, 780, 807, 1345, 1725, 2616, 3264
Sau96I GGNCC 5 cut(s) 740, 1274, 2369, 2525, 3166
SchI GAGTC 6 cut(s) 521, 851, 1121, 1214, 1634, 2276
ScrFI CCNGG 7 cut(s) 731, 744, 889, 1140, 2005, 3465, 3509
SduI GDGCHC 2 cut(s) 81, 693
SexAI ACCWGGT 2 cut(s) 742, 1138
SfcI CTRYAG 4 cut(s) 1734, 3183, 3513, 3627
SinI GGWCC 2 cut(s) 740, 2369
SmiMI CAYNNNNRTG 4 cut(s) 131, 1581, 2763, 3129
SmlI CTYRAG 5 cut(s) 1131, 1261, 1800, 2429, 3034
SmoI CTYRAG 5 cut(s) 1131, 1261, 1800, 2429, 3034
SseBI AGGCCT 1 cut(s) 794
SsiI CCGC 8 cut(s) 140, 360, 613, 1345, 1375, 1466, 2606, 2873
SspI AATATT 4 cut(s) 607, 1669, 1991, 2972
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 7 cut(s) 729, 742, 887, 1138, 2003, 3463, 3507
StyI CCWWGG 4 cut(s) 1225, 1411, 2726, 3502
TaiI ACGT 6 cut(s) 2180, 2197, 2587, 2674, 2707, 3406
TatI WGTACW 1 cut(s) 815
TauI GCSGC 2 cut(s) 615, 1347
TfiI GAWTC 7 cut(s) 563, 877, 959, 1529, 1603, 2867, 2897
TscAI CASTG 9 cut(s) 408, 418, 505, 511, 844, 1618, 2257, 2898, 3282
TseFI GTSAC 6 cut(s) 500, 2587, 2675, 3029, 3280, 3769
TseI GCWGC 6 cut(s) 704, 779, 806, 1724, 2615, 3263
Tsp45I GTSAC 6 cut(s) 500, 2587, 2675, 3029, 3280, 3769
TspGWI ACGGA 5 cut(s) 662, 1055, 2829, 3017, 3719
TspRI CASTG 9 cut(s) 408, 418, 505, 511, 844, 1618, 2257, 2898, 3282
Van91I CCANNNNNTGG 5 cut(s) 241, 268, 322, 349, 3127
Vha464I CTTAAG 1 cut(s) 3034
VpaK11BI GGWCC 2 cut(s) 740, 2369
VspI ATTAAT 3 cut(s) 239, 590, 1671
XapI RAATTY 7 cut(s) 1044, 1181, 1367, 2696, 2811, 3552, 3597
XceI RCATGY 1 cut(s) 3388
XmiI GTMKAC 1 cut(s) 1560
XmnI GAANNNNTTC 1 cut(s) 1858
Zsp2I ATGCAT 3 cut(s) 199, 2248, 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.