Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003450

Physical Location & Seq

Genomic Coordinates Forward (+)

16126116 .. 16134436

Gene Structure 8321 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1188900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGCTGTCGAATCATTTGCAGAACGGGATCGGGGCGCCGAAGCTCGCCTGGTCCCGCCTCCCGCACTCGGAGGAAGCCGCCGAGCCCGACGCCGTCGGTCTCATGAAGAAGAGCGACGATGTCGGCGTCGAGAGCCTTGACTACGAAGTTATTGAAAATTACGCATATTGGGAGGAGCAGGCGCAGAGAGGCAAGCTCTATGTTGGGTATCATGTGATGGTGAAGTGGTTCTTTGCTTTGCTCATCGGTATCGGCACTGGATTAGCTGCGGTTTTCATCAATATTGCAGTCGAGAACTTTGCAGGCTGGAAGTTTGCATTGACATTTGCAATAATCCAAAAATCATATGTGGCTGGTTTTGTAGTATACATATTAATCAACTTAGTTTTGGTTTTTTCGTCTGTATATATCGTTACACAATTTGCACCCGCAGCAGCTGGGTCCGGTATTCCCGAAATCAAGGGGTATTTGAATGGGGTTGATATACACGGTGTACTTCTTTTCAGAACCTTGATTGGAAAGATCTTTGGGAGCATTGGCTCCGTGGGAGGTGGTCTAGCTTTAGGCAAAGAGGGCCCTCTTGTTCATACTGGTGCTTGTATTGCGTCTTTGCTTGGACAAGGTGGATCTACGAAATACCATCTAAGCTCTAGGTGGCTACAGATATTTAATAGCGATCGGGATCGTAGGGATCTGGTAACCTGTGGGTGTGCAGCTGGAGTGTCTGCTGCTTTTCGAGCTCCCGTTGGTGGTGTTCTATTTGCACTGGAAGAAGTTACATCTTGGTGGAAGAGTCAACTTATGTGGCGTGTATTTTTTACTTCTGCTATTGTGGCTGTTGTGGTGCGTACTGCGATGGGATGGTGTAAGAGTGGAAAGTGTGGACATTTTGGCTCAGGTGGCTTCATAATATGGGACTTATCAGATGGTCAAGAGGACTACTCTTTTGAGGAGTTCTTGCCGATGGCGGTTATTGGGGTCATTGGAGGATTACTGGGAGCCTTATTTAACCAGCTTACGTTTTATATTGCATACTGGCGTCGAAATTATTTGCACAAGAATGGGAATCGAGTCAAGATAATTGAAGCGTGCCTCATCTCGCTGATAACATCAATTATTTCCTTTGGTCTACCACTTTTACGAAAATGCAGTCCATGCCCTGAAGCAGATCTGGATTCTGGTATTGAATGCCCAAGGCCTCCAGGAACGTATGGGAATTATGTAAATTTTTATTGCAGTAAGAACAAGGAATACAATGATCTCGCAACTATTTTCTTCAATACTCAGGATGATGCAATTAGGAATTTGTTTAGTGCAAAAACAGTTCATGAGTATAGTGCCCAAAGTTTGTTGACCTTCTTGGTGATGTTTTATTCATTAGCAGTTGTGACATTTGGTACTGCCGTTCCAGCTGGTCAATTTGTTCCTGGGATCATGATAGGATCTACATATGGGCGTCTTGTGGGCAAGTTTGTGGTTAGCTTTTACAAGAAGCTCAACATTGAAGAGGGAACATATGCTCTACTGGGAGCAGCTTCTTTTCTTGGAGGTTCAATGCGGATGACAGTGTCTCTGTGCGTCATTATGGTTGAAATCACAAATAATTTGAAATTATTGCCTCTTATCATGCTTGTCCTTCTTATTTCAAAGGCTGTTGGTGATGCTTTCAATGAAGGCCTGTACGAAGAACAGGCGCGATTAAAGAGCATTCCATTACTGGAATCAAGACCCAAGTACCAAATGAGGAAGATGACTGCAAAAGAGGCATCTGGAAAAAGGGTGATTTCCTTCCCTCGTGTTGTTAAGGTTGCAGATGTGGTTTCTATTTTGCGGAGCAACAATCATAATGGCTTCCCTGTGATTGATCATGCAAGAAATGGGGAAACACTTGTAATTGGACTAATGCTTCGTAGTCACTTGTTGGTACTTCTGCAGTCGAAGGTGGATTTCCAACATAGTCCTTTACCCTGTGATCCAAGAGGATCCAGGTCTATCAGGCATACCATCAGTGAATTTGTGAAACCTGCTTCCAGTAAAGGCTTATCGATGAATGATGTACATCTGAGTTCAGATGACTTAGAAATGTACATAGATCTTGCCCCATTTTTGAACCCATCTCCTTACATTGTCCCTGAGGATATGTCTTTGACAAAGGTATATAATCTTTTCCGACAACTAGGTCTGAGGCATATATTTGTTGTTCCCCGTCCATCTCGTGTGCTTGGCTTGATTACCAGGAAGGATTTATTGCTTGAGGAGTATGAGGATCCAGCTTCGGTGGAGCTCCAATCGACTAGTGTAAGAACTCAGCATCGAGATAGAAGAAAGGTTGGAAGGAATGGAGATATGGAGCGCCCACTTCTCACTAGTCTTCTGGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

794

Amino Acids

87.51

Weight (kDa)

8.76

Isoelectric Point (pI)

40.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	131 - 553	7.9e-86	Voltage gated chloride channel
CBS	PF00571	704 - 751	3e-07	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26240 AT5G26240
fragaria_vesca	FvH4_4g15130 FvH4_4g15130
malus_domestica	MD13G1188900.v1.1 MD16G1189700.v1.1
prunus_persica	Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1
pyrus_communis	pycom13g16330 pycom16g15940
rosa_chinensis	RchiOBHm_Chr3g0497631 RchiOBHm_Chr4g0416741
rosa_laevigata	RLG00000007956 RLG00000023042
rosa_multiflora	Rmu_co8329069.1_g000001 Rmu_sc0023201.1_g000001 Rmu_sc0025051.1_g000001 Rmu_sc0038555.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360610 Rroxscaffold_6G00395290
rosa_rugosa	Rorug04G0144200 Rorug04G0144200 Rorug04G0144200 Rorug04G0144200
rosa_samantha	Rh1BG229600 Rh1CG042200 Rh1DG027400 Rh4AG207000 Rh4BG204200 Rh7CG094800
rosa_wichuraiana	Rw4G017510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2178
Acc36I	ACCTGC	1 cut(s)	2032
AccB1I	GGYRCC	1 cut(s)	34
AccI	GTMKAC	2 cut(s)	366, 1129
AccII	CGCG	1 cut(s)	1696
AciI	CCGC	8 cut(s)	55, 62, 78, 269, 429, 968, 1558, 1831
AcsI	RAATTY	3 cut(s)	1225, 1303, 2012
AcuI	CTGAAG	1 cut(s)	1182
AcyI	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
AfaI	GTAC	8 cut(s)	495, 850, 1399, 1682, 1736, 1926, 2058, 2087
AfiI	CCNNNNNNNGG	2 cut(s)	67, 749
AhlI	ACTAGT	2 cut(s)	2294, 2366
AjnI	CCWGG	5 cut(s)	47, 1201, 1426, 1985, 2234
Alw21I	GWGCWC	2 cut(s)	742, 2286
Alw26I	GTCTC	2 cut(s)	104, 1575
AlwNI	CAGNNNCTG	1 cut(s)	437
AoxI	GGCC	3 cut(s)	574, 1196, 1675
ApaI	GGGCCC	1 cut(s)	578
ApeKI	GCWGC	6 cut(s)	266, 431, 434, 713, 728, 1532
ApoI	RAATTY	3 cut(s)	1225, 1303, 2012
ArsI	GACNNNNNNTTYG	6 cut(s)	929, 961, 1135, 1167, 1890, 1922
AseI	ATTAAT	1 cut(s)	374
Asp700I	GAANNNNTTC	1 cut(s)	227
AspLEI	GCGC	4 cut(s)	37, 184, 1696, 2355
AspS9I	GGNCC	4 cut(s)	51, 441, 574, 575
AsuHPI	GGTGA	4 cut(s)	232, 1375, 1670, 1792
AvaII	GGWCC	2 cut(s)	51, 441
AxyI	CCTNAGG	1 cut(s)	2133
BaeGI	GKGCMC	2 cut(s)	578, 1342
BaeI	ACNNNNGTAYC	4 cut(s)	1389, 1422, 1718, 1751
BamHI	GGATCC	2 cut(s)	1982, 2266
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	4 cut(s)	87, 578, 742, 2286
BauI	CACGAG	2 cut(s)	1794, 2214
BbsI	GAAGAC	1 cut(s)	2363
Bbv12I	GWGCWC	2 cut(s)	742, 2286
BbvI	GCAGC	6 cut(s)	253, 443, 446, 715, 725, 1544
BccI	CCATC	9 cut(s)	211, 648, 850, 855, 920, 958, 2012, 2122, 2218
BceAI	ACGGC	2 cut(s)	77, 1388
BcgI	CGANNNNNNTGC	3 cut(s)	32, 281, 315
BciT130I	CCWGG	5 cut(s)	49, 1203, 1428, 1987, 2236
BclI	TGATCA	1 cut(s)	1864
BcoDI	GTCTC	2 cut(s)	104, 1575
BcuI	ACTAGT	2 cut(s)	2294, 2366
BfaI	CTAG	5 cut(s)	557, 651, 2177, 2295, 2367
BfmI	CTRYAG	2 cut(s)	659, 1931
BfoI	RGCGCY	2 cut(s)	38, 2356
BfuAI	ACCTGC	1 cut(s)	2032
BglII	AGATCT	3 cut(s)	522, 1168, 2092
BisI	GCNGC	7 cut(s)	78, 267, 432, 435, 714, 729, 1533
BlsI	GCNGC	7 cut(s)	79, 268, 433, 436, 715, 730, 1534
Bme1390I	CCNGG	5 cut(s)	49, 1203, 1428, 1987, 2236
Bme18I	GGWCC	2 cut(s)	51, 441
BmgT120I	GGNCC	4 cut(s)	51, 441, 574, 575
BmiI	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 1000, 1984, 2268
BmrFI	CCNGG	5 cut(s)	49, 1203, 1428, 1987, 2236
BmrI	ACTGGG	2 cut(s)	1004, 1535
BmsI	GCATC	4 cut(s)	1282, 1651, 1775, 2320
BmuI	ACTGGG	2 cut(s)	1004, 1535
BpiI	GAAGAC	1 cut(s)	2363
BplI	GAGNNNNNCTC	4 cut(s)	180, 212, 926, 958
BpmI	CTGGAG	2 cut(s)	738, 1185
Bpu10I	CCTNAGC	1 cut(s)	895
BpuEI	CTTGAG	1 cut(s)	2273
Bsa29I	ATCGAT	1 cut(s)	2045
BsaBI	GATNNNNATC	2 cut(s)	681, 2058
BsaHI	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
BsaI	GGTCTC	1 cut(s)	104
BsaJI	CCNNGG	3 cut(s)	543, 1193, 1427
BsaWI	WCCGGW	1 cut(s)	443
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 749
Bse1I	ACTGG	8 cut(s)	262, 595, 771, 999, 1040, 1530, 1722, 2031
Bse21I	CCTNAGG	1 cut(s)	2133
Bse8I	GATNNNNATC	2 cut(s)	681, 2058
BseBI	CCWGG	5 cut(s)	49, 1203, 1428, 1987, 2236
BseCI	ATCGAT	1 cut(s)	2045
BseDI	CCNNGG	3 cut(s)	543, 1193, 1427
BseGI	GGATG	3 cut(s)	866, 1294, 1566
BseJI	GATNNNNATC	2 cut(s)	681, 2058
BseLI	CCNNNNNNNGG	2 cut(s)	67, 749
BseMII	CTCAG	6 cut(s)	909, 1298, 2054, 2124, 2174, 2321
BseNI	ACTGG	8 cut(s)	262, 595, 771, 999, 1040, 1530, 1722, 2031
BseRI	GAGGAG	3 cut(s)	188, 965, 2270
BseSI	GKGCMC	2 cut(s)	578, 1342
BseXI	GCAGC	6 cut(s)	253, 443, 446, 715, 725, 1544
BseYI	CCCAGC	1 cut(s)	437
BsgI	GTGCAG	1 cut(s)	732
Bsh1236I	CGCG	1 cut(s)	1696
Bsh1285I	CGRYCG	1 cut(s)	679
BshFI	GGCC	3 cut(s)	576, 1198, 1677
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	2045
BsiEI	CGRYCG	1 cut(s)	679
BsiHKAI	GWGCWC	2 cut(s)	742, 2286
BsiSI	CCGG	1 cut(s)	444
BslFI	GGGAC	3 cut(s)	37, 929, 2114
BslI	CCNNNNNNNGG	2 cut(s)	67, 749
BsmAI	GTCTC	2 cut(s)	104, 1575
BsmFI	GGGAC	3 cut(s)	37, 929, 2114
BsmI	GAATGC	2 cut(s)	1193, 1707
BsnI	GGCC	3 cut(s)	576, 1198, 1677
Bso31I	GGTCTC	1 cut(s)	104
Bsp120I	GGGCCC	1 cut(s)	574
Bsp1286I	GDGCHC	5 cut(s)	87, 578, 742, 1342, 2286
Bsp1407I	TGTACA	2 cut(s)	2056, 2085
BspACI	CCGC	8 cut(s)	55, 62, 78, 269, 429, 968, 1558, 1831
BspANI	GGCC	3 cut(s)	576, 1198, 1677
BspCNI	CTCAG	6 cut(s)	908, 1297, 2055, 2125, 2175, 2320
BspDI	ATCGAT	1 cut(s)	2045
BspFNI	CGCG	1 cut(s)	1696
BspHI	TCATGA	3 cut(s)	102, 1327, 1434
BspLI	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 1000, 1984, 2268
BspMAI	CTGCAG	1 cut(s)	1935
BspMI	ACCTGC	1 cut(s)	2032
BspQI	GCTCTTC	1 cut(s)	104
BspT107I	GGYRCC	1 cut(s)	34
BspTNI	GGTCTC	1 cut(s)	104
BsrGI	TGTACA	2 cut(s)	2056, 2085
BsrI	ACTGG	8 cut(s)	262, 595, 771, 999, 1040, 1530, 1722, 2031
BssECI	CCNNGG	3 cut(s)	543, 1193, 1427
BssNAI	GTATAC	1 cut(s)	367
BssNI	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
BssSI	CACGAG	2 cut(s)	1794, 2214
BssT1I	CCWWGG	1 cut(s)	1193
Bst1107I	GTATAC	1 cut(s)	367
Bst2BI	CACGAG	2 cut(s)	1794, 2214
Bst2UI	CCWGG	5 cut(s)	49, 1203, 1428, 1987, 2236
Bst4CI	ACNGT	3 cut(s)	491, 1324, 1567
Bst6I	CTCTTC	3 cut(s)	104, 785, 1500
BstACI	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
BstAPI	GCANNNNNTGC	1 cut(s)	1155
BstAUI	TGTACA	2 cut(s)	2056, 2085
BstC8I	GCNNGC	5 cut(s)	45, 180, 194, 304, 1090
BstDEI	CTNAG	9 cut(s)	382, 644, 895, 1284, 2063, 2077, 2133, 2183, 2307
BstDSI	CCRYGG	1 cut(s)	543
BstEII	GGTNACC	1 cut(s)	697
BstF5I	GGATG	3 cut(s)	866, 1294, 1566
BstFNI	CGCG	1 cut(s)	1696
BstH2I	RGCGCY	2 cut(s)	38, 2356
BstHHI	GCGC	4 cut(s)	37, 184, 1696, 2355
BstMAI	GTCTC	2 cut(s)	104, 1575
BstMCI	CGRYCG	1 cut(s)	679
BstNI	CCWGG	5 cut(s)	49, 1203, 1428, 1987, 2236
BstPI	GGTNACC	1 cut(s)	697
BstSCI	CCNGG	5 cut(s)	47, 1201, 1426, 1985, 2234
BstSFI	CTRYAG	2 cut(s)	659, 1931
BstSLI	GKGCMC	2 cut(s)	578, 1342
BstUI	CGCG	1 cut(s)	1696
BstV1I	GCAGC	6 cut(s)	253, 443, 446, 715, 725, 1544
BstV2I	GAAGAC	1 cut(s)	2363
BstX2I	RGATCY	8 cut(s)	522, 626, 691, 1168, 1442, 1982, 2092, 2266
BstYI	RGATCY	8 cut(s)	522, 626, 691, 1168, 1442, 1982, 2092, 2266
BstZ17I	GTATAC	1 cut(s)	367
Bsu15I	ATCGAT	1 cut(s)	2045
Bsu36I	CCTNAGG	1 cut(s)	2133
BsuRI	GGCC	3 cut(s)	576, 1198, 1677
BsuTUI	ATCGAT	1 cut(s)	2045
BtgI	CCRYGG	1 cut(s)	543
BtgZI	GCGATG	1 cut(s)	869
BtsCI	GGATG	3 cut(s)	866, 1294, 1566
BtsIMutI	CAGTG	4 cut(s)	255, 764, 1572, 2014
BveI	ACCTGC	1 cut(s)	2032
Cac8I	GCNNGC	5 cut(s)	45, 180, 194, 304, 1090
CaiI	CAGNNNCTG	1 cut(s)	437
CciI	TCATGA	3 cut(s)	102, 1327, 1434
CfoI	GCGC	4 cut(s)	37, 184, 1696, 2355
Cfr13I	GGNCC	4 cut(s)	51, 441, 574, 575
ClaI	ATCGAT	1 cut(s)	2045
CseI	GACGC	6 cut(s)	98, 115, 594, 1028, 1445, 1567
Csp6I	GTAC	8 cut(s)	494, 849, 1398, 1681, 1735, 1925, 2057, 2086
CspCI	CAANNNNNGTGG	2 cut(s)	785, 820
CviAII	CATG	7 cut(s)	103, 212, 1155, 1328, 1435, 1627, 1868
CviQI	GTAC	8 cut(s)	494, 849, 1398, 1681, 1735, 1925, 2057, 2086
DdeI	CTNAG	9 cut(s)	382, 644, 895, 1284, 2063, 2077, 2133, 2183, 2307
DinI	GGCGCC	1 cut(s)	36
DrdI	GACNNNNNNGTC	1 cut(s)	2178
DseDI	GACNNNNNNGTC	1 cut(s)	2178
Eam1104I	CTCTTC	3 cut(s)	104, 785, 1500
EarI	CTCTTC	3 cut(s)	104, 785, 1500
Ecl136II	GAGCTC	2 cut(s)	740, 2284
Eco130I	CCWWGG	1 cut(s)	1193
Eco147I	AGGCCT	2 cut(s)	1198, 1677
Eco24I	GRGCYC	4 cut(s)	87, 578, 742, 2286
Eco31I	GGTCTC	1 cut(s)	104
Eco47I	GGWCC	2 cut(s)	51, 441
Eco53kI	GAGCTC	2 cut(s)	740, 2284
Eco57I	CTGAAG	1 cut(s)	1182
Eco81I	CCTNAGG	1 cut(s)	2133
Eco91I	GGTNACC	1 cut(s)	697
EcoICRI	GAGCTC	2 cut(s)	740, 2284
EcoO109I	RGGNCCY	2 cut(s)	574, 575
EcoO65I	GGTNACC	1 cut(s)	697
EcoRII	CCWGG	5 cut(s)	47, 1201, 1426, 1985, 2234
EcoT14I	CCWWGG	1 cut(s)	1193
EcoT38I	GRGCYC	4 cut(s)	87, 578, 742, 2286
EgeI	GGCGCC	1 cut(s)	36
EheI	GGCGCC	1 cut(s)	36
ErhI	CCWWGG	1 cut(s)	1193
FaeI	CATG	7 cut(s)	106, 215, 1158, 1331, 1438, 1630, 1871
FalI	AAGNNNNNCTT	2 cut(s)	215, 247
FaqI	GGGAC	3 cut(s)	37, 929, 2114
FatI	CATG	7 cut(s)	102, 211, 1154, 1327, 1434, 1626, 1867
FauI	CCCGC	3 cut(s)	62, 69, 436
FauNDI	CATATG	3 cut(s)	346, 1450, 1516
FbaI	TGATCA	1 cut(s)	1864
FblI	GTMKAC	2 cut(s)	366, 1129
Fnu4HI	GCNGC	7 cut(s)	78, 267, 432, 435, 714, 729, 1533
FokI	GGATG	3 cut(s)	873, 1301, 1573
FriOI	GRGCYC	4 cut(s)	87, 578, 742, 2286
Fsp4HI	GCNGC	7 cut(s)	78, 267, 432, 435, 714, 729, 1533
FspBI	CTAG	5 cut(s)	557, 651, 2177, 2295, 2367
GlaI	GCGC	4 cut(s)	36, 183, 1695, 2354
GluI	GCNGC	7 cut(s)	78, 267, 432, 435, 714, 729, 1533
GsaI	CCCAGC	1 cut(s)	441
GsuI	CTGGAG	2 cut(s)	738, 1185
HaeII	RGCGCY	2 cut(s)	38, 2356
HaeIII	GGCC	3 cut(s)	576, 1198, 1677
HapII	CCGG	1 cut(s)	444
HgaI	GACGC	6 cut(s)	98, 115, 594, 1028, 1445, 1567
HhaI	GCGC	4 cut(s)	37, 184, 1696, 2355
Hin1I	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
Hin1II	CATG	7 cut(s)	106, 215, 1158, 1331, 1438, 1630, 1871
Hin6I	GCGC	4 cut(s)	35, 182, 1694, 2353
HinP1I	GCGC	4 cut(s)	35, 182, 1694, 2353
HincII	GTYRAC	2 cut(s)	797, 1353
HindII	GTYRAC	2 cut(s)	797, 1353
HinfI	GANTC	6 cut(s)	10, 793, 1066, 1071, 1175, 1721
HpaII	CCGG	1 cut(s)	444
HphI	GGTGA	4 cut(s)	232, 1375, 1670, 1792
Hpy166II	GTNNAC	6 cut(s)	367, 494, 797, 884, 1130, 1353
Hpy188I	TCNGA	7 cut(s)	70, 506, 925, 2064, 2071, 2171, 2184
Hpy8I	GTNNAC	6 cut(s)	367, 494, 797, 884, 1130, 1353
Hpy99I	CGWCG	5 cut(s)	92, 98, 119, 131, 1044
HpyAV	CCTTC	7 cut(s)	1366, 1646, 1667, 1798, 1933, 2233, 2328
HpyCH4III	ACNGT	3 cut(s)	491, 1324, 1567
HpyCH4IV	ACGT	2 cut(s)	1019, 1208
HpyF3I	CTNAG	9 cut(s)	382, 644, 895, 1284, 2063, 2077, 2133, 2183, 2307
HpySE526I	ACGT	2 cut(s)	1019, 1208
Hsp92I	GRCGYC	5 cut(s)	35, 90, 126, 1039, 1456
Hsp92II	CATG	7 cut(s)	106, 215, 1158, 1331, 1438, 1630, 1871
HspAI	GCGC	4 cut(s)	35, 182, 1694, 2353
KasI	GGCGCC	1 cut(s)	34
Ksp22I	TGATCA	1 cut(s)	1864
LguI	GCTCTTC	1 cut(s)	104
Lsp1109I	GCAGC	6 cut(s)	253, 443, 446, 715, 725, 1544
LweI	GCATC	4 cut(s)	1282, 1651, 1775, 2320
MaeI	CTAG	5 cut(s)	557, 651, 2177, 2295, 2367
MaeII	ACGT	2 cut(s)	1019, 1208
MaeIII	GTNAC	5 cut(s)	412, 697, 775, 1387, 1913
MflI	RGATCY	8 cut(s)	522, 626, 691, 1168, 1442, 1982, 2092, 2266
MhlI	GDGCHC	5 cut(s)	87, 578, 742, 1342, 2286
Mly113I	GGCGCC	1 cut(s)	35
MlyI	GAGTC	2 cut(s)	802, 1080
MmeI	TCCRAC	3 cut(s)	1975, 2194, 2311
MroXI	GAANNNNTTC	1 cut(s)	227
MseI	TTAA	5 cut(s)	374, 669, 1008, 1700, 1803
MslI	CAYNNNNRTG	3 cut(s)	591, 784, 1059
MspA1I	CMGCKG	3 cut(s)	437, 716, 1412
MspI	CCGG	1 cut(s)	444
MspR9I	CCNGG	5 cut(s)	49, 1203, 1428, 1987, 2236
Mva1269I	GAATGC	2 cut(s)	1193, 1707
MvaI	CCWGG	5 cut(s)	49, 1203, 1428, 1987, 2236
MvnI	CGCG	1 cut(s)	1696
NarI	GGCGCC	1 cut(s)	35
NdeI	CATATG	3 cut(s)	346, 1450, 1516
NlaIII	CATG	7 cut(s)	106, 215, 1158, 1331, 1438, 1630, 1871
NlaIV	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 1000, 1984, 2268
NmeAIII	GCCGAG	1 cut(s)	106
NmuCI	GTSAC	2 cut(s)	1387, 1913
PagI	TCATGA	3 cut(s)	102, 1327, 1434
PceI	AGGCCT	2 cut(s)	1198, 1677
PciSI	GCTCTTC	1 cut(s)	104
PcsI	WCGNNNNNNNCGW	1 cut(s)	123
PctI	GAATGC	2 cut(s)	1193, 1707
PdmI	GAANNNNTTC	1 cut(s)	227
PfeI	GAWTC	4 cut(s)	10, 1066, 1175, 1721
PflFI	GACNNNGTC	3 cut(s)	92, 119, 1567
PfoI	TCCNGGA	1 cut(s)	1201
PkrI	GCNGC	7 cut(s)	79, 268, 433, 436, 715, 730, 1534
Ple19I	CGATCG	1 cut(s)	679
PleI	GAGTC	2 cut(s)	801, 1079
PluTI	GGCGCC	1 cut(s)	38
PpsI	GAGTC	2 cut(s)	801, 1079
PshBI	ATTAAT	1 cut(s)	374
Psp124BI	GAGCTC	2 cut(s)	742, 2286
Psp6I	CCWGG	5 cut(s)	47, 1201, 1426, 1985, 2234
PspEI	GGTNACC	1 cut(s)	697
PspFI	CCCAGC	1 cut(s)	437
PspGI	CCWGG	5 cut(s)	47, 1201, 1426, 1985, 2234
PspN4I	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 1000, 1984, 2268
PspOMI	GGGCCC	1 cut(s)	574
PspPI	GGNCC	4 cut(s)	51, 441, 574, 575
PsrI	GAACNNNNNNTAC	2 cut(s)	1235, 1267
PstI	CTGCAG	1 cut(s)	1935
PstNI	CAGNNNCTG	1 cut(s)	437
PsuI	RGATCY	8 cut(s)	522, 626, 691, 1168, 1442, 1982, 2092, 2266
PsyI	GACNNNGTC	3 cut(s)	92, 119, 1567
PvuI	CGATCG	1 cut(s)	679
PvuII	CAGCTG	3 cut(s)	437, 716, 1412
RsaI	GTAC	8 cut(s)	495, 850, 1399, 1682, 1736, 1926, 2058, 2087
RsaNI	GTAC	8 cut(s)	494, 849, 1398, 1681, 1735, 1925, 2057, 2086
RseI	CAYNNNNRTG	3 cut(s)	591, 784, 1059
SacI	GAGCTC	2 cut(s)	742, 2286
SapI	GCTCTTC	1 cut(s)	104
SaqAI	TTAA	5 cut(s)	374, 669, 1008, 1700, 1803
SatI	GCNGC	7 cut(s)	78, 267, 432, 435, 714, 729, 1533
Sau96I	GGNCC	4 cut(s)	51, 441, 574, 575
SchI	GAGTC	2 cut(s)	802, 1080
ScrFI	CCNGG	5 cut(s)	49, 1203, 1428, 1987, 2236
SduI	GDGCHC	5 cut(s)	87, 578, 742, 1342, 2286
SfaNI	GCATC	4 cut(s)	1282, 1651, 1775, 2320
SfcI	CTRYAG	2 cut(s)	659, 1931
SfoI	GGCGCC	1 cut(s)	36
SinI	GGWCC	2 cut(s)	51, 441
SmiMI	CAYNNNNRTG	3 cut(s)	591, 784, 1059
SmlI	CTYRAG	1 cut(s)	2252
SmoI	CTYRAG	1 cut(s)	2252
SpeI	ACTAGT	2 cut(s)	2294, 2366
SseBI	AGGCCT	2 cut(s)	1198, 1677
SsiI	CCGC	8 cut(s)	55, 62, 78, 269, 429, 968, 1558, 1831
SspDI	GGCGCC	1 cut(s)	34
SspI	AATATT	1 cut(s)	283
SspMI	CTAG	5 cut(s)	557, 651, 2177, 2295, 2367
SstI	GAGCTC	2 cut(s)	742, 2286
StuI	AGGCCT	2 cut(s)	1198, 1677
StyD4I	CCNGG	5 cut(s)	47, 1201, 1426, 1985, 2234
StyI	CCWWGG	1 cut(s)	1193
TaaI	ACNGT	3 cut(s)	491, 1324, 1567
TaiI	ACGT	2 cut(s)	1022, 1211
TaqII	GACCGA	1 cut(s)	86
TatI	WGTACW	3 cut(s)	493, 2056, 2085
TauI	GCSGC	1 cut(s)	80
TfiI	GAWTC	4 cut(s)	10, 1066, 1175, 1721
Tru1I	TTAA	5 cut(s)	374, 669, 1008, 1700, 1803
Tru9I	TTAA	5 cut(s)	374, 669, 1008, 1700, 1803
TscAI	CASTG	4 cut(s)	262, 771, 1572, 2014
TseFI	GTSAC	2 cut(s)	1387, 1913
TseI	GCWGC	6 cut(s)	266, 431, 434, 713, 728, 1532
Tsp45I	GTSAC	2 cut(s)	1387, 1913
TspDTI	ATGAA	8 cut(s)	119, 265, 575, 895, 1316, 1365, 1686, 2063
TspGWI	ACGGA	1 cut(s)	532
TspRI	CASTG	4 cut(s)	262, 771, 1572, 2014
Tth111I	GACNNNGTC	3 cut(s)	92, 119, 1567
VpaK11BI	GGWCC	2 cut(s)	51, 441
VspI	ATTAAT	1 cut(s)	374
XapI	RAATTY	3 cut(s)	1225, 1303, 2012
XmiI	GTMKAC	2 cut(s)	366, 1129
XmnI	GAANNNNTTC	1 cut(s)	227
XspI	CTAG	5 cut(s)	557, 651, 2177, 2295, 2367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.