Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003450

Physical Location & Seq

Genomic Coordinates Forward (+)

23413920 .. 23420833

Gene Structure 6914 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007956

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGCTGTCGAATCACTTGCAGAATGGGATCGAGGCGCCGAAGCTGGCCTGGTCTCGCCTCCCGAACTCGGACGAAGCCGAGAACGACGTCTTTGGGTTGAGGAAGAAGGACGACGAGGGCGCCGGCGTCGAGAGCCTTGACTATGAAGTCATCGAGAACTACGCTTATTGGGAAGAGCAGGCCCAGAGAGGGAAGCTCTATGTTGGATATTATGTGGCTGTCAAGTGGTTCTTTGCTTTGCTTATCGGCATCGGCACTGGATTAGCTGCTGTTTTCATCAATATTTCGGTTGAGAACTTTGCAGGCTGGAAATTTGCATTGACATTTGCTATAATACAAAAATCATATCTGGCCGGTTTCTTGGTGTACATATTAATCAACTTAGTTTTGGTTTTTTCCTCTGTATATATCATTACACAATTTGCACCGGCAGCAGCTGGGTCTGGTATTCCAGAAATCAAGGGGTATTTAAATGGGGTCGACATACATGGTGTCCTACTTTTCAGAACGTTGATTGGAAAGATATTTGGGAGCATTGGTTCTGTGGGAGGAGGTCTAGCTTTAGGCAAAGAAGGTCCTCTTGTTCATACTGGTGCTTGTATTGCATCCTTGCTTGGACAAGGTGGATCTACAAAATACCATCTCAGATCCAGGTGGCTACAAATTTTTAATAGTGACCGAGATCGCCGTGATCTTGTAACCTGTGGATGTGCAGCTGGAGTTTCTGCTGCTTTTAGAGCTCCTGTAGGTGGTGTACTATTTGCACTGGAAGAAGTTACATCTTGGTGGAGGAGTCAACTTATGTGGCGTGTGTTTTTCACTTCTGCTATTGTGGCTGTTGTAGTGCGTACTGCAATGGGATGGTGCAAGAGTGGAAAATGTGGACATTTTGGCTCAGGTGGCTTCATAATATGGGATTTATCAGATGGTCAAGAGGACTATTCTTTTGAGGAGTTCTTGCCAATGGCGGTTATTGGGGTCATTGGAGGTTTACTGGGAGCCTTGTTTAACCAGCTTACATTTTACATTGCTCACTGGCGCCGAAATTATTTGCACAAGAAAGGGAATCGAGTCAAGATAATTGAAGCGTGTCTCATCTCTCTGATAACGTCAATTATTTCCTTCGGTTTACCACTTTTAAGAAAATGTAGTCAATGCCCTGAAGCAGATCCAGATTCTGGTATTGAATGCCCGCGGCCTCCAGGAATGTATGGAAATTATGTTAATTTTTATTGCAGCAAGGACAGGGAATACAATGATCTTGCAACTATTTTCTTCAATACTCAGGATGATGCGATCAGGAATTTGTTTAGTGCAAAAACAATACATGAATACAGTGCCCAAAGTTTATTGACCTTCTTGGTTATGTTTTACACATTAGCAGTTGTGACATTTGGTACTGCTGTTCCAGCTGGTCAATTTGTTCCTGGAATAATGATAGGATCAACATATGGACGTCTTGTAGGCATGTTTGTTGTTAGCTTCTACAAGAAGCTCAACATTGAAGAGGGAACATATGCACTACTTGGAGCAGCTTCTTTTCTTGGAGGTTCAATGCGAATGACGGTGTCTCTATGTGTCATTATGGTTGAAATCACAAATAATCTGAAATTGTTGCCTCTTATCATGCTTGTTCTTCTTATTTCAAAGGCTGTTGGAGATGCATTCAACGAAGGCCTGTATGAAGAACAGTCACGGTTGAGGAACATTCCACAGTTAGAATCAAAACCCAAATATCAAATGCGGAAGATGACAGCAAAGGAGGCCTGTGGAAAAAGGGTGATTTCCTTCCCTCGCGTTGTTAAGGTTGCAGATTTGGTTTCTATTCTACGGAGCAACATCCACAATGGCTTCCCTTCGAAGGTAGATTTCCAGCATAGCCCTTTGCCATGTGATCCCAGAGGATCTAGGTCTATCAGGCACACTTTGAGCGAGTTTGTGAAACCTGCTACCAGTAAAGGATTATCAATACATGATATACATCTGAGCTCAGACGACTTGGAAATGTACATAGATCTTGCCCCCTTTTTAAACCCATCTCCTTACATCGTCCCCGAGGATATGTCTTTGACAAAGGTATATAATCTTTTCCGTCAGCTAGGTCTGAGACACATATTTGTTGTTCCGCGCCCATCTCGTGTGATTGGTTTGATTACCAGAAAGGATTTATTGATTGAGGAGAATGAGGATTCTGCTTCAATGGAACTCCAATCGACTAGTGTAAGAGCACAACATCCTGATAGAAGAAAGGGTAGGAGGATTGGTGATATGGAGCACCCGCTACTCACTGGTCTTCTGTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

768

Amino Acids

84.98

Weight (kDa)

8.59

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	131 - 553	9.6e-87	Voltage gated chloride channel
CBS	PF00571	678 - 724	1.9e-07	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26240 AT5G26240
fragaria_vesca	FvH4_4g15130 FvH4_4g15130
malus_domestica	MD13G1188900.v1.1 MD16G1189700.v1.1
prunus_persica	Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1
pyrus_communis	pycom13g16330 pycom16g15940
rosa_chinensis	RchiOBHm_Chr3g0497631 RchiOBHm_Chr4g0416741
rosa_laevigata	RLG00000007956 RLG00000023042
rosa_multiflora	Rmu_co8329069.1_g000001 Rmu_sc0023201.1_g000001 Rmu_sc0025051.1_g000001 Rmu_sc0038555.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360610 Rroxscaffold_6G00395290
rosa_rugosa	Rorug04G0144200 Rorug04G0144200 Rorug04G0144200 Rorug04G0144200
rosa_samantha	Rh1BG229600 Rh1CG042200 Rh1DG027400 Rh4AG207000 Rh4BG204200 Rh7CG094800
rosa_wichuraiana	Rw4G017510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	146
AatII	GACGTC	2 cut(s)	90, 1459
Acc36I	ACCTGC	1 cut(s)	1954
AccB1I	GGYRCC	3 cut(s)	34, 119, 1038
AccB7I	CCANNNNNTGG	1 cut(s)	1178
AccI	GTMKAC	1 cut(s)	480
AccII	CGCG	3 cut(s)	1195, 1797, 2128
AciI	CCGC	6 cut(s)	968, 1193, 1195, 1744, 2126, 2279
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	7 cut(s)	35, 634, 642, 1163, 1450, 1889, 1912
AcoI	YGGCCR	1 cut(s)	351
AcsI	RAATTY	3 cut(s)	311, 663, 1303
AcuI	CTGAAG	1 cut(s)	1182
AcyI	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
AfaI	GTAC	5 cut(s)	368, 756, 850, 1399, 2009
AfiI	CCNNNNNNNGG	5 cut(s)	67, 189, 749, 1178, 1861
AgsI	TTSAA	9 cut(s)	1085, 1187, 1279, 1505, 1554, 1592, 1647, 1669, 2199
AhlI	ACTAGT	1 cut(s)	2216
AjnI	CCWGG	4 cut(s)	47, 650, 1201, 1426
AloI	GAACNNNNNNTCC	2 cut(s)	523, 555
Alw21I	GWGCWC	4 cut(s)	742, 1991, 2230, 2277
Alw26I	GTCTC	4 cut(s)	57, 1097, 1575, 2101
AlwI	GGATC	7 cut(s)	35, 634, 642, 1163, 1450, 1889, 1912
AlwNI	CAGNNNCTG	2 cut(s)	437, 1178
Ama87I	CYCGRG	1 cut(s)	2054
AoxI	GGCC	6 cut(s)	45, 180, 351, 1196, 1675, 1764
ApeKI	GCWGC	7 cut(s)	266, 431, 434, 713, 728, 1236, 1532
ApoI	RAATTY	3 cut(s)	311, 663, 1303
ArsI	GACNNNNNNTTYG	2 cut(s)	929, 961
AseI	ATTAAT	1 cut(s)	374
AspLEI	GCGC	4 cut(s)	37, 122, 1041, 2130
AspS9I	GGNCC	2 cut(s)	181, 575
AsuHPI	GGTGA	2 cut(s)	1792, 2276
AsuII	TTCGAA	1 cut(s)	1859
AvaI	CYCGRG	1 cut(s)	2054
AvaII	GGWCC	1 cut(s)	575
BaeGI	GKGCMC	1 cut(s)	1342
BaeI	ACNNNNGTAYC	2 cut(s)	1389, 1422
BanI	GGYRCC	3 cut(s)	34, 119, 1038
BanII	GRGCYC	2 cut(s)	742, 1991
BauI	CACGAG	1 cut(s)	2136
BbsI	GAAGAC	1 cut(s)	2285
Bbv12I	GWGCWC	4 cut(s)	742, 1991, 2230, 2277
BbvI	GCAGC	7 cut(s)	253, 443, 446, 715, 725, 1248, 1544
BccI	CCATC	5 cut(s)	648, 855, 920, 2044, 2140
BceAI	ACGGC	1 cut(s)	672
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	4 cut(s)	49, 652, 1203, 1428
BcoDI	GTCTC	4 cut(s)	57, 1097, 1575, 2101
BcuI	ACTAGT	1 cut(s)	2216
BfaI	CTAG	4 cut(s)	557, 1908, 2099, 2217
BfmI	CTRYAG	1 cut(s)	744
BfoI	RGCGCY	3 cut(s)	38, 123, 1042
BfuAI	ACCTGC	1 cut(s)	1954
BglII	AGATCT	1 cut(s)	2014
BisI	GCNGC	8 cut(s)	267, 432, 435, 714, 729, 1196, 1237, 1533
BlsI	GCNGC	8 cut(s)	268, 433, 436, 715, 730, 1197, 1238, 1534
Bme1390I	CCNGG	4 cut(s)	49, 652, 1203, 1428
Bme18I	GGWCC	1 cut(s)	575
BmeT110I	CYCGRG	1 cut(s)	2054
BmgT120I	GGNCC	2 cut(s)	181, 575
BmiI	GGNNCC	4 cut(s)	36, 121, 1000, 1040
BmrFI	CCNGG	4 cut(s)	49, 652, 1203, 1428
BmrI	ACTGGG	1 cut(s)	1004
BmsI	GCATC	4 cut(s)	258, 614, 1282, 1651
BmuI	ACTGGG	1 cut(s)	1004
BpiI	GAAGAC	1 cut(s)	2285
BplI	GAGNNNNNCTC	2 cut(s)	180, 212
BpmI	CTGGAG	2 cut(s)	738, 1185
Bpu10I	CCTNAGC	1 cut(s)	895
Bpu14I	TTCGAA	1 cut(s)	1859
BsaBI	GATNNNNATC	1 cut(s)	1980
BsaHI	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
BsaI	GGTCTC	1 cut(s)	57
BsaJI	CCNNGG	2 cut(s)	1193, 2055
BsaXI	ACNNNNNCTCC	4 cut(s)	523, 553, 2248, 2278
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 189, 749, 1178, 1861
Bse118I	RCCGGY	3 cut(s)	122, 353, 427
Bse1I	ACTGG	7 cut(s)	262, 595, 771, 999, 1040, 1953, 2293
Bse3DI	GCAATG	2 cut(s)	861, 1026
Bse8I	GATNNNNATC	1 cut(s)	1980
BseBI	CCWGG	4 cut(s)	49, 652, 1203, 1428
BseDI	CCNNGG	2 cut(s)	1193, 2055
BseGI	GGATG	6 cut(s)	605, 713, 866, 1294, 1839, 2233
BseJI	GATNNNNATC	1 cut(s)	1980
BseLI	CCNNNNNNNGG	5 cut(s)	67, 189, 749, 1178, 1861
BseMI	GCAATG	2 cut(s)	861, 1026
BseMII	CTCAG	6 cut(s)	658, 909, 1298, 1976, 2004, 2096
BseNI	ACTGG	7 cut(s)	262, 595, 771, 999, 1040, 1953, 2293
BseRI	GAGGAG	4 cut(s)	564, 805, 965, 2192
BseSI	GKGCMC	1 cut(s)	1342
BseXI	GCAGC	7 cut(s)	253, 443, 446, 715, 725, 1248, 1544
BseYI	CCCAGC	1 cut(s)	437
BsgI	GTGCAG	1 cut(s)	732
Bsh1236I	CGCG	3 cut(s)	1195, 1797, 2128
BshFI	GGCC	6 cut(s)	47, 182, 353, 1198, 1677, 1766
BshNI	GGYRCC	3 cut(s)	34, 119, 1038
BsiHKAI	GWGCWC	4 cut(s)	742, 1991, 2230, 2277
BsiHKCI	CYCGRG	1 cut(s)	2054
BsiSI	CCGG	3 cut(s)	123, 354, 428
BslFI	GGGAC	1 cut(s)	2036
BslI	CCNNNNNNNGG	5 cut(s)	67, 189, 749, 1178, 1861
BsmAI	GTCTC	4 cut(s)	57, 1097, 1575, 2101
BsmFI	GGGAC	1 cut(s)	2036
BsmI	GAATGC	2 cut(s)	1193, 1664
BsnI	GGCC	6 cut(s)	47, 182, 353, 1198, 1677, 1766
Bso31I	GGTCTC	1 cut(s)	57
BsoBI	CYCGRG	1 cut(s)	2054
Bsp119I	TTCGAA	1 cut(s)	1859
Bsp1286I	GDGCHC	5 cut(s)	742, 1342, 1991, 2230, 2277
Bsp1407I	TGTACA	2 cut(s)	366, 2007
BspACI	CCGC	6 cut(s)	968, 1193, 1195, 1744, 2126, 2279
BspANI	GGCC	6 cut(s)	47, 182, 353, 1198, 1677, 1766
BspCNI	CTCAG	6 cut(s)	657, 908, 1297, 1977, 2003, 2097
BspFNI	CGCG	3 cut(s)	1195, 1797, 2128
BspLI	GGNNCC	4 cut(s)	36, 121, 1000, 1040
BspMI	ACCTGC	1 cut(s)	1954
BspPI	GGATC	7 cut(s)	35, 634, 642, 1163, 1450, 1889, 1912
BspQI	GCTCTTC	1 cut(s)	168
BspT104I	TTCGAA	1 cut(s)	1859
BspT107I	GGYRCC	3 cut(s)	34, 119, 1038
BspTNI	GGTCTC	1 cut(s)	57
BsrDI	GCAATG	2 cut(s)	861, 1026
BsrFI	RCCGGY	3 cut(s)	122, 353, 427
BsrGI	TGTACA	2 cut(s)	366, 2007
BsrI	ACTGG	7 cut(s)	262, 595, 771, 999, 1040, 1953, 2293
BssAI	RCCGGY	3 cut(s)	122, 353, 427
BssECI	CCNNGG	2 cut(s)	1193, 2055
BssNI	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
BssSI	CACGAG	1 cut(s)	2136
Bst2BI	CACGAG	1 cut(s)	2136
Bst2UI	CCWGG	4 cut(s)	49, 652, 1203, 1428
Bst4CI	ACNGT	5 cut(s)	1337, 1567, 1692, 1698, 1716
Bst6I	CTCTTC	2 cut(s)	168, 1500
BstACI	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
BstAUI	TGTACA	2 cut(s)	366, 2007
BstBI	TTCGAA	1 cut(s)	1859
BstC8I	GCNNGC	5 cut(s)	45, 124, 180, 304, 1193
BstDEI	CTNAG	7 cut(s)	382, 644, 895, 1284, 1985, 1990, 2105
BstDSI	CCRYGG	1 cut(s)	1193
BstF5I	GGATG	6 cut(s)	605, 713, 866, 1294, 1839, 2233
BstFNI	CGCG	3 cut(s)	1195, 1797, 2128
BstH2I	RGCGCY	3 cut(s)	38, 123, 1042
BstHHI	GCGC	4 cut(s)	37, 122, 1041, 2130
BstMAI	GTCTC	4 cut(s)	57, 1097, 1575, 2101
BstMWI	GCNNNNNNNGC	8 cut(s)	132, 431, 602, 737, 833, 900, 1409, 1763
BstNI	CCWGG	4 cut(s)	49, 652, 1203, 1428
BstNSI	RCATGY	1 cut(s)	1471
BstSCI	CCNGG	4 cut(s)	47, 650, 1201, 1426
BstSFI	CTRYAG	1 cut(s)	744
BstSLI	GKGCMC	1 cut(s)	1342
BstUI	CGCG	3 cut(s)	1195, 1797, 2128
BstV1I	GCAGC	7 cut(s)	253, 443, 446, 715, 725, 1248, 1544
BstV2I	GAAGAC	1 cut(s)	2285
BstX2I	RGATCY	5 cut(s)	626, 647, 1168, 1904, 2014
BstYI	RGATCY	5 cut(s)	626, 647, 1168, 1904, 2014
BsuRI	GGCC	6 cut(s)	47, 182, 353, 1198, 1677, 1766
BtgI	CCRYGG	1 cut(s)	1193
BtsCI	GGATG	6 cut(s)	605, 713, 866, 1294, 1839, 2233
BtsIMutI	CAGTG	5 cut(s)	255, 764, 1033, 1342, 2286
BveI	ACCTGC	1 cut(s)	1954
Cac8I	GCNNGC	5 cut(s)	45, 124, 180, 304, 1193
CaiI	CAGNNNCTG	2 cut(s)	437, 1178
CfoI	GCGC	4 cut(s)	37, 122, 1041, 2130
Cfr10I	RCCGGY	3 cut(s)	122, 353, 427
Cfr13I	GGNCC	2 cut(s)	181, 575
Cfr42I	CCGCGG	1 cut(s)	1196
CseI	GACGC	1 cut(s)	115
Csp6I	GTAC	5 cut(s)	367, 755, 849, 1398, 2008
CspCI	CAANNNNNGTGG	2 cut(s)	785, 820
CviAII	CATG	6 cut(s)	488, 1328, 1468, 1627, 1890, 1973
CviQI	GTAC	5 cut(s)	367, 755, 849, 1398, 2008
DdeI	CTNAG	7 cut(s)	382, 644, 895, 1284, 1985, 1990, 2105
DinI	GGCGCC	3 cut(s)	36, 121, 1040
DraI	TTTAAA	2 cut(s)	471, 2031
DrdI	GACNNNNNNGTC	1 cut(s)	146
DseDI	GACNNNNNNGTC	1 cut(s)	146
EaeI	YGGCCR	1 cut(s)	351
Eam1104I	CTCTTC	2 cut(s)	168, 1500
EarI	CTCTTC	2 cut(s)	168, 1500
Ecl136II	GAGCTC	2 cut(s)	740, 1989
Eco147I	AGGCCT	2 cut(s)	1677, 1766
Eco24I	GRGCYC	2 cut(s)	742, 1991
Eco31I	GGTCTC	1 cut(s)	57
Eco47I	GGWCC	1 cut(s)	575
Eco53kI	GAGCTC	2 cut(s)	740, 1989
Eco57I	CTGAAG	1 cut(s)	1182
Eco88I	CYCGRG	1 cut(s)	2054
EcoICRI	GAGCTC	2 cut(s)	740, 1989
EcoO109I	RGGNCCY	1 cut(s)	575
EcoRII	CCWGG	4 cut(s)	47, 650, 1201, 1426
EcoT22I	ATGCAT	1 cut(s)	1666
EcoT38I	GRGCYC	2 cut(s)	742, 1991
EgeI	GGCGCC	3 cut(s)	36, 121, 1040
EheI	GGCGCC	3 cut(s)	36, 121, 1040
FaeI	CATG	6 cut(s)	491, 1331, 1471, 1630, 1893, 1976
FalI	AAGNNNNNCTT	4 cut(s)	215, 247, 564, 596
FaqI	GGGAC	1 cut(s)	2036
FatI	CATG	6 cut(s)	487, 1327, 1467, 1626, 1889, 1972
FauI	CCCGC	2 cut(s)	1200, 2286
FauNDI	CATATG	2 cut(s)	1450, 1516
FblI	GTMKAC	1 cut(s)	480
Fnu4HI	GCNGC	8 cut(s)	267, 432, 435, 714, 729, 1196, 1237, 1533
FokI	GGATG	6 cut(s)	592, 720, 873, 1301, 1826, 2220
FriOI	GRGCYC	2 cut(s)	742, 1991
Fsp4HI	GCNGC	8 cut(s)	267, 432, 435, 714, 729, 1196, 1237, 1533
FspBI	CTAG	4 cut(s)	557, 1908, 2099, 2217
GlaI	GCGC	4 cut(s)	36, 121, 1040, 2129
GluI	GCNGC	8 cut(s)	267, 432, 435, 714, 729, 1196, 1237, 1533
GsaI	CCCAGC	1 cut(s)	441
GsuI	CTGGAG	2 cut(s)	738, 1185
HaeII	RGCGCY	3 cut(s)	38, 123, 1042
HaeIII	GGCC	6 cut(s)	47, 182, 353, 1198, 1677, 1766
HapII	CCGG	3 cut(s)	123, 354, 428
HgaI	GACGC	1 cut(s)	115
HhaI	GCGC	4 cut(s)	37, 122, 1041, 2130
Hin1I	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
Hin1II	CATG	6 cut(s)	491, 1331, 1471, 1630, 1893, 1976
Hin6I	GCGC	4 cut(s)	35, 120, 1039, 2128
HinP1I	GCGC	4 cut(s)	35, 120, 1039, 2128
HincII	GTYRAC	2 cut(s)	481, 797
HindII	GTYRAC	2 cut(s)	481, 797
HinfI	GANTC	7 cut(s)	10, 793, 1066, 1071, 1175, 1721, 2189
HpaII	CCGG	3 cut(s)	123, 354, 428
HphI	GGTGA	2 cut(s)	1792, 2276
Hpy166II	GTNNAC	7 cut(s)	367, 481, 755, 797, 884, 992, 1130
Hpy188I	TCNGA	9 cut(s)	70, 506, 647, 925, 1104, 1608, 1986, 1993, 2106
Hpy8I	GTNNAC	7 cut(s)	367, 481, 755, 797, 884, 992, 1130
Hpy99I	CGWCG	3 cut(s)	89, 116, 131
HpyAV	CCTTC	8 cut(s)	100, 566, 1132, 1366, 1667, 1798, 1855, 1866
HpyCH4III	ACNGT	5 cut(s)	1337, 1567, 1692, 1698, 1716
HpyCH4IV	ACGT	4 cut(s)	87, 509, 1109, 1456
HpyF10VI	GCNNNNNNNGC	8 cut(s)	132, 431, 602, 737, 833, 900, 1409, 1763
HpyF3I	CTNAG	7 cut(s)	382, 644, 895, 1284, 1985, 1990, 2105
HpySE526I	ACGT	4 cut(s)	87, 509, 1109, 1456
Hsp92I	GRCGYC	6 cut(s)	35, 87, 120, 126, 1039, 1456
Hsp92II	CATG	6 cut(s)	491, 1331, 1471, 1630, 1893, 1976
HspAI	GCGC	4 cut(s)	35, 120, 1039, 2128
KasI	GGCGCC	3 cut(s)	34, 119, 1038
KroI	GCCGGC	1 cut(s)	122
KroNI	GCCGGC	1 cut(s)	124
KspI	CCGCGG	1 cut(s)	1196
LguI	GCTCTTC	1 cut(s)	168
LmnI	GCTCC	6 cut(s)	531, 745, 998, 1529, 1833, 2272
Lsp1109I	GCAGC	7 cut(s)	253, 443, 446, 715, 725, 1248, 1544
LweI	GCATC	4 cut(s)	258, 614, 1282, 1651
MaeI	CTAG	4 cut(s)	557, 1908, 2099, 2217
MaeII	ACGT	4 cut(s)	87, 509, 1109, 1456
MaeIII	GTNAC	5 cut(s)	674, 697, 775, 1387, 1692
MflI	RGATCY	5 cut(s)	626, 647, 1168, 1904, 2014
MhlI	GDGCHC	5 cut(s)	742, 1342, 1991, 2230, 2277
Mly113I	GGCGCC	3 cut(s)	35, 120, 1039
MlyI	GAGTC	2 cut(s)	802, 1080
MmeI	TCCRAC	2 cut(s)	184, 1636
Mph1103I	ATGCAT	1 cut(s)	1666
MreI	CGCCGGCG	1 cut(s)	122
MroNI	GCCGGC	1 cut(s)	122
MseI	TTAA	8 cut(s)	374, 470, 669, 1008, 1139, 1224, 1803, 2030
MslI	CAYNNNNRTG	2 cut(s)	591, 784
MspA1I	CMGCKG	4 cut(s)	437, 716, 1195, 1412
MspI	CCGG	3 cut(s)	123, 354, 428
MspR9I	CCNGG	4 cut(s)	49, 652, 1203, 1428
Mva1269I	GAATGC	2 cut(s)	1193, 1664
MvaI	CCWGG	4 cut(s)	49, 652, 1203, 1428
MvnI	CGCG	3 cut(s)	1195, 1797, 2128
MwoI	GCNNNNNNNGC	8 cut(s)	132, 431, 602, 737, 833, 900, 1409, 1763
NaeI	GCCGGC	1 cut(s)	124
NarI	GGCGCC	3 cut(s)	35, 120, 1039
NdeI	CATATG	2 cut(s)	1450, 1516
NgoMIV	GCCGGC	1 cut(s)	122
NlaIII	CATG	6 cut(s)	491, 1331, 1471, 1630, 1893, 1976
NlaIV	GGNNCC	4 cut(s)	36, 121, 1000, 1040
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	3 cut(s)	674, 1387, 1692
NsiI	ATGCAT	1 cut(s)	1666
NspI	RCATGY	1 cut(s)	1471
NspV	TTCGAA	1 cut(s)	1859
PceI	AGGCCT	2 cut(s)	1677, 1766
PciSI	GCTCTTC	1 cut(s)	168
PctI	GAATGC	2 cut(s)	1193, 1664
PdiI	GCCGGC	1 cut(s)	124
PfeI	GAWTC	5 cut(s)	10, 1066, 1175, 1721, 2189
PflFI	GACNNNGTC	1 cut(s)	1567
PflMI	CCANNNNNTGG	1 cut(s)	1178
PfoI	TCCNGGA	2 cut(s)	1201, 1426
PkrI	GCNGC	8 cut(s)	268, 433, 436, 715, 730, 1197, 1238, 1534
PleI	GAGTC	2 cut(s)	801, 1079
PluTI	GGCGCC	3 cut(s)	38, 123, 1042
PpsI	GAGTC	2 cut(s)	801, 1079
PpuMI	RGGWCCY	1 cut(s)	575
PshBI	ATTAAT	1 cut(s)	374
Psp124BI	GAGCTC	2 cut(s)	742, 1991
Psp1406I	AACGTT	1 cut(s)	509
Psp5II	RGGWCCY	1 cut(s)	575
Psp6I	CCWGG	4 cut(s)	47, 650, 1201, 1426
PspFI	CCCAGC	1 cut(s)	437
PspGI	CCWGG	4 cut(s)	47, 650, 1201, 1426
PspN4I	GGNNCC	4 cut(s)	36, 121, 1000, 1040
PspPI	GGNCC	2 cut(s)	181, 575
PspPPI	RGGWCCY	1 cut(s)	575
PstNI	CAGNNNCTG	2 cut(s)	437, 1178
PsuI	RGATCY	5 cut(s)	626, 647, 1168, 1904, 2014
PsyI	GACNNNGTC	1 cut(s)	1567
PvuII	CAGCTG	3 cut(s)	437, 716, 1412
RsaI	GTAC	5 cut(s)	368, 756, 850, 1399, 2009
RsaNI	GTAC	5 cut(s)	367, 755, 849, 1398, 2008
RseI	CAYNNNNRTG	2 cut(s)	591, 784
SacI	GAGCTC	2 cut(s)	742, 1991
SacII	CCGCGG	1 cut(s)	1196
SalI	GTCGAC	1 cut(s)	479
SapI	GCTCTTC	1 cut(s)	168
SaqAI	TTAA	8 cut(s)	374, 470, 669, 1008, 1139, 1224, 1803, 2030
SatI	GCNGC	8 cut(s)	267, 432, 435, 714, 729, 1196, 1237, 1533
Sau96I	GGNCC	2 cut(s)	181, 575
SchI	GAGTC	2 cut(s)	802, 1080
ScrFI	CCNGG	4 cut(s)	49, 652, 1203, 1428
SduI	GDGCHC	5 cut(s)	742, 1342, 1991, 2230, 2277
SfaNI	GCATC	4 cut(s)	258, 614, 1282, 1651
SfcI	CTRYAG	1 cut(s)	744
SfoI	GGCGCC	3 cut(s)	36, 121, 1040
Sfr303I	CCGCGG	1 cut(s)	1196
SfuI	TTCGAA	1 cut(s)	1859
SgrAI	CRCCGGYG	1 cut(s)	122
SgrBI	CCGCGG	1 cut(s)	1196
SinI	GGWCC	1 cut(s)	575
SmiI	ATTTAAAT	1 cut(s)	471
SmiMI	CAYNNNNRTG	2 cut(s)	591, 784
SpeI	ACTAGT	1 cut(s)	2216
SseBI	AGGCCT	2 cut(s)	1677, 1766
SsiI	CCGC	6 cut(s)	968, 1193, 1195, 1744, 2126, 2279
SspDI	GGCGCC	3 cut(s)	34, 119, 1038
SspI	AATATT	1 cut(s)	283
SspMI	CTAG	4 cut(s)	557, 1908, 2099, 2217
SstI	GAGCTC	2 cut(s)	742, 1991
StuI	AGGCCT	2 cut(s)	1677, 1766
StyD4I	CCNGG	4 cut(s)	47, 650, 1201, 1426
SwaI	ATTTAAAT	1 cut(s)	471
TaaI	ACNGT	5 cut(s)	1337, 1567, 1692, 1698, 1716
TaiI	ACGT	4 cut(s)	90, 512, 1112, 1459
TaqI	TCGA	8 cut(s)	8, 30, 129, 153, 480, 1069, 1859, 2213
TaqII	GACCGA	1 cut(s)	693
TatI	WGTACW	3 cut(s)	366, 754, 2007
TauI	GCSGC	1 cut(s)	1198
TfiI	GAWTC	5 cut(s)	10, 1066, 1175, 1721, 2189
Tru1I	TTAA	8 cut(s)	374, 470, 669, 1008, 1139, 1224, 1803, 2030
Tru9I	TTAA	8 cut(s)	374, 470, 669, 1008, 1139, 1224, 1803, 2030
TscAI	CASTG	5 cut(s)	262, 771, 1040, 1342, 2293
TseFI	GTSAC	3 cut(s)	674, 1387, 1692
TseI	GCWGC	7 cut(s)	266, 431, 434, 713, 728, 1236, 1532
Tsp45I	GTSAC	3 cut(s)	674, 1387, 1692
TspDTI	ATGAA	6 cut(s)	159, 265, 575, 895, 1344, 1698
TspGWI	ACGGA	2 cut(s)	1846, 2081
TspRI	CASTG	5 cut(s)	262, 771, 1040, 1342, 2293
Tth111I	GACNNNGTC	1 cut(s)	1567
Van91I	CCANNNNNTGG	1 cut(s)	1178
VpaK11BI	GGWCC	1 cut(s)	575
VspI	ATTAAT	1 cut(s)	374
XapI	RAATTY	3 cut(s)	311, 663, 1303
XceI	RCATGY	1 cut(s)	1471
XmiI	GTMKAC	1 cut(s)	480
XspI	CTAG	4 cut(s)	557, 1908, 2099, 2217
ZraI	GACGTC	2 cut(s)	88, 1457
Zsp2I	ATGCAT	1 cut(s)	1666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.