Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003450

Physical Location & Seq

Genomic Coordinates Forward (+)

16505299 .. 16520614

Gene Structure 15316 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1189700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGCTGTCGAATCATTTGCAGAACGGGATCGAGGGGCCGAAGATCTCCTGGTCCCGCCTCCCGAGCTCGGAGGGCGGCGTCGAGCCCGACGCTGTCGGCCTCATGAAGAAGAGCGACGATGGCGGCGTCGAGAGCCTTGATTATGAAGTTATTGAAAATTACGCTTATTGGGATGAGCAGGCGCAAAGAGGGAAGCTCTATGTTGGGTATCACGTGATAGTGAAGTTTTTCTTTGCTTTGCTCATTGGTATCGGGACTGGATTAGCTGCTGTTTTCATTAATATTGCGGTCGAGAACTTTGCAGGCTGGAAGTTTGCATTGACATTTGCAATAATCCAAAAATCATATGTGGCTGGTTTTGTAGTATACATATTGATCAACTTAATTTTGGTTTTTTCGTCTGTATATATCGTTACACAATTTGCACCCGCAGCAGCTGGGTCCGGTATTCCAGAAATCAAGGGATATTTGAATGGGGTTGATATACACGGTGTACTTCTTTTCAGAACCTTGGTTGGAAAGATATTTGGGAGCATTGGCTCCGTGGGAGGTGGTCTAGCTTTAGGCAAGGAGGGTCCTCTTGTTCATACCGGTGCTTGTATTGCTTCTTTGCTTGGACAACTTCTGGAGTGGTGGATACAAATTTTTAATAGTGACCGGGATCGTCGGGATCTGGTAACCTGTGGGTGTGCAGCTGGAGTGTCTGCTGCTTTTAGAGCTCCCGTTGGTGGTGTTCTATTTGCACTGGAAGAAGTTACATCGTGGTTGGGGTATGACTACTCTTTTGAGGAGTTCTTGCCGATGGCAGTTATTGGGGTCATTGGTGGTGTACTGGGAGCCTTATTTAACCAGCTTACATTTTATATTGCGTACTGGCGCCGAAATTATTTGCACAAGAATGGGAATCGAGTAAAGATAATTGAAGCGTGCCTCATCTCGCTGATAACATCAATTATTTCCTTTGGTCTACCACTTTTAAGAAAATGCAGTCCATGCCCTGAAGCAGATGCAGATTCTGGTATTGAATGCCCAAGGCCTCCGGGAACGTATGGGAATTATGTAAATTTTTATTGCAGTAAGGACAAGGAGTACAATGATCTTGCAACTATTTTCTTCAATACTCAGGATGATGCAATTAGGAATTTGTTTAGTGCAAAAACATTTCACGAGTACAGTGCCCAAAGTTTATTGACTTTCTTGGTGATGTTTTATTCATTAGCGGTTGTGACATTTGGTACTGCCGTTCCAGCTGGTCAATTTGTTCCTGGGATAATGATAGGATCTACATATGGGCGTCTTGTGGGCATGTTTGTGGTTAACTTTTACAAGAAGCTCAGCATCGAAGAGGGAACATATGCACTACTGGGAGCAGCTTCTTTTCTTGGAGGTTCAATGCGGATGACAGTGTCTCTTTGTGTCATTATGGTTGAAATCACAAATAATTTGAAACTTTTGCCTCTTATCATGCTTGTTCTTCTTATTTCAAAGGCTGTTGGTGATGCTTTCAACGAAGGCCTGTACGAAGAACAGGTGCGATTAAAGAGCATTCCATTACTGGAATCAAGACCCAAGTACCAAATGAGGAAGATGCCTGCAAAGGAGGCCTCTGGAAAAAGGGTGATTTCCTTCCCTCGTGTTGTTAAGGTTGCAGATTTGGTTTCTATTTTGCGGAGCAACAATCATAATGGCTTCCCTGTGATTGATCATGCAAGAAGTGGAGAAACACTTGTAATTGGACTAATGCTTCGCAGTCACTTGTTGGTACTTCTGCAGTCCAAAGTGGATTTCCAGCATAGTCCTCTACCCTGTGATCCAAGAGGAGCCAGGCACACTATCAGTGAATTCGTAAAACCTGCTTCCAGTAAAGGCTTATTGATAAGTGATATACATCTGAGTTCAGATGACTTGGAAATGTACATAGATCTTGCGCCCTTTTTGAACCCATCTCCTTACATTGTCCCTGAAGATATGTCTTTGCCAAAGGTGAGAGTTTCGCTGCCATTCTTTCAACACTTTTCTTTCATGGAATGTATATATCTTTTCCGTCAACTAGGTTTAAGACATATATTTGTTACTCCTCGTCCATCTTGTGTGATTGGCTTGATTACCAGGAAGGATTTATTACTCGGGGAGAATGAGGATTCAGCATCGATGGAGCTCCAATTGACTAGTAACTCGACATCGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

730

Amino Acids

80.22

Weight (kDa)

6.71

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	131 - 255	2e-37	Voltage gated chloride channel
Voltage_CLC	PF00654	259 - 499	1e-38	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26240 AT5G26240
fragaria_vesca	FvH4_4g15130 FvH4_4g15130
malus_domestica	MD13G1188900.v1.1 MD16G1189700.v1.1
prunus_persica	Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1 Prupe.1G156000_v2.0.a1
pyrus_communis	pycom13g16330 pycom16g15940
rosa_chinensis	RchiOBHm_Chr3g0497631 RchiOBHm_Chr4g0416741
rosa_laevigata	RLG00000007956 RLG00000023042
rosa_multiflora	Rmu_co8329069.1_g000001 Rmu_sc0023201.1_g000001 Rmu_sc0025051.1_g000001 Rmu_sc0038555.1_g000001
rosa_roxburghii	Rroxscaffold_5G00360610 Rroxscaffold_6G00395290
rosa_rugosa	Rorug04G0144200 Rorug04G0144200 Rorug04G0144200 Rorug04G0144200
rosa_samantha	Rh1BG229600 Rh1CG042200 Rh1DG027400 Rh4AG207000 Rh4BG204200 Rh7CG094800
rosa_wichuraiana	Rw4G017510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1861
AccB1I	GGYRCC	1 cut(s)	876
AccI	GTMKAC	2 cut(s)	366, 967
AciI	CCGC	8 cut(s)	55, 75, 123, 287, 429, 1220, 1396, 1669
AclWI	GGATC	5 cut(s)	35, 669, 678, 1288, 1805
AcsI	RAATTY	4 cut(s)	642, 1063, 1141, 1841
AcuI	CTGAAG	2 cut(s)	1020, 1983
AcvI	CACGTG	1 cut(s)	214
AcyI	GRCGYC	4 cut(s)	78, 126, 877, 1294
AfiI	CCNNNNNNNGG	2 cut(s)	67, 728
AgeI	ACCGGT	1 cut(s)	590
AhlI	ACTAGT	1 cut(s)	2168
AjnI	CCWGG	4 cut(s)	47, 1264, 1823, 2108
AjuI	GAANNNNNNNTTGG	2 cut(s)	1769, 1801
Alw21I	GWGCWC	3 cut(s)	68, 721, 2160
Alw26I	GTCTC	1 cut(s)	1413
AlwI	GGATC	5 cut(s)	35, 669, 678, 1288, 1805
AlwNI	CAGNNNCTG	2 cut(s)	437, 1016
Ama87I	CYCGRG	2 cut(s)	61, 2126
AoxI	GGCC	5 cut(s)	35, 97, 1034, 1513, 1602
ApeKI	GCWGC	7 cut(s)	266, 431, 434, 692, 707, 1370, 1996
ApoI	RAATTY	4 cut(s)	642, 1063, 1141, 1841
ArsI	GACNNNNNNTTYG	4 cut(s)	767, 799, 1728, 1760
AseI	ATTAAT	1 cut(s)	279
AsiGI	ACCGGT	1 cut(s)	590
Asp700I	GAANNNNTTC	1 cut(s)	227
AspLEI	GCGC	3 cut(s)	184, 879, 1930
AspS9I	GGNCC	4 cut(s)	35, 51, 441, 575
AsuC2I	CCSGG	2 cut(s)	659, 1041
AsuHPI	GGTGA	4 cut(s)	1213, 1508, 1630, 1996
AvaI	CYCGRG	2 cut(s)	61, 2126
AvaII	GGWCC	3 cut(s)	51, 441, 575
BaeGI	GKGCMC	1 cut(s)	1180
BaeI	ACNNNNGTAYC	4 cut(s)	1227, 1260, 1556, 1589
BanI	GGYRCC	1 cut(s)	876
BanII	GRGCYC	4 cut(s)	68, 87, 721, 2160
BauI	CACGAG	2 cut(s)	1166, 1632
BbrPI	CACGTG	1 cut(s)	214
Bbv12I	GWGCWC	3 cut(s)	68, 721, 2160
BbvI	GCAGC	7 cut(s)	253, 443, 446, 694, 704, 1382, 1983
BccI	CCATC	5 cut(s)	113, 796, 1951, 2092, 2146
BceAI	ACGGC	1 cut(s)	1226
BcgI	CGANNNNNNTGC	5 cut(s)	32, 281, 315, 1157, 1191
BciT130I	CCWGG	4 cut(s)	49, 1266, 1825, 2110
BciVI	GTATCC	1 cut(s)	630
BclI	TGATCA	2 cut(s)	375, 1702
BcnI	CCSGG	2 cut(s)	659, 1041
BcoDI	GTCTC	1 cut(s)	1413
BcuI	ACTAGT	1 cut(s)	2168
BfaI	CTAG	3 cut(s)	557, 2051, 2169
BfmI	CTRYAG	1 cut(s)	1769
BfoI	RGCGCY	1 cut(s)	880
BfuAI	ACCTGC	1 cut(s)	1861
BfuI	GTATCC	1 cut(s)	630
BglII	AGATCT	2 cut(s)	42, 1921
BisI	GCNGC	9 cut(s)	76, 124, 267, 432, 435, 693, 708, 1371, 1997
BlpI	GCTNAGC	1 cut(s)	1334
BlsI	GCNGC	9 cut(s)	77, 125, 268, 433, 436, 694, 709, 1372, 1998
Bme1390I	CCNGG	6 cut(s)	49, 659, 1041, 1266, 1825, 2110
Bme18I	GGWCC	3 cut(s)	51, 441, 575
BmeT110I	CYCGRG	2 cut(s)	61, 2126
BmgT120I	GGNCC	4 cut(s)	35, 51, 441, 575
BmiI	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 838, 878, 1822
BmrFI	CCNGG	6 cut(s)	49, 659, 1041, 1266, 1825, 2110
BmrI	ACTGGG	2 cut(s)	842, 1373
BmsI	GCATC	6 cut(s)	997, 1120, 1347, 1489, 1578, 2156
BmuI	ACTGGG	2 cut(s)	842, 1373
BplI	GAGNNNNNCTC	2 cut(s)	180, 212
BpmI	CTGGAG	2 cut(s)	647, 717
Bpu1102I	GCTNAGC	1 cut(s)	1334
BpuMI	CCSGG	2 cut(s)	659, 1041
Bsa29I	ATCGAT	1 cut(s)	2150
BsaAI	YACGTR	1 cut(s)	214
BsaBI	GATNNNNATC	1 cut(s)	1887
BsaHI	GRCGYC	4 cut(s)	78, 126, 877, 1294
BsaJI	CCNNGG	4 cut(s)	510, 543, 1031, 1265
BsaWI	WCCGGW	2 cut(s)	443, 590
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 728
Bse118I	RCCGGY	1 cut(s)	590
Bse1I	ACTGG	7 cut(s)	262, 750, 837, 878, 1368, 1560, 1860
Bse8I	GATNNNNATC	1 cut(s)	1887
BseBI	CCWGG	4 cut(s)	49, 1266, 1825, 2110
BseCI	ATCGAT	1 cut(s)	2150
BseDI	CCNNGG	4 cut(s)	510, 543, 1031, 1265
BseGI	GGATG	3 cut(s)	178, 1132, 1404
BseJI	GATNNNNATC	1 cut(s)	1887
BseLI	CCNNNNNNNGG	2 cut(s)	67, 728
BseMII	CTCAG	3 cut(s)	1136, 1348, 1883
BseNI	ACTGG	7 cut(s)	262, 750, 837, 878, 1368, 1560, 1860
BseRI	GAGGAG	3 cut(s)	803, 1833, 2067
BseSI	GKGCMC	1 cut(s)	1180
BseXI	GCAGC	7 cut(s)	253, 443, 446, 694, 704, 1382, 1983
BseYI	CCCAGC	1 cut(s)	437
BsgI	GTGCAG	1 cut(s)	711
Bsh1285I	CGRYCG	1 cut(s)	291
BshFI	GGCC	5 cut(s)	37, 99, 1036, 1515, 1604
BshNI	GGYRCC	1 cut(s)	876
BshTI	ACCGGT	1 cut(s)	590
BshVI	ATCGAT	1 cut(s)	2150
BsiEI	CGRYCG	1 cut(s)	291
BsiHKAI	GWGCWC	3 cut(s)	68, 721, 2160
BsiHKCI	CYCGRG	2 cut(s)	61, 2126
BsiSI	CCGG	4 cut(s)	444, 591, 658, 1040
BslFI	GGGAC	3 cut(s)	37, 268, 1943
BslI	CCNNNNNNNGG	2 cut(s)	67, 728
BsmAI	GTCTC	1 cut(s)	1413
BsmFI	GGGAC	3 cut(s)	37, 268, 1943
BsmI	GAATGC	2 cut(s)	1031, 1545
BsnI	GGCC	5 cut(s)	37, 99, 1036, 1515, 1604
BsoBI	CYCGRG	2 cut(s)	61, 2126
Bsp1286I	GDGCHC	5 cut(s)	68, 87, 721, 1180, 2160
Bsp1407I	TGTACA	1 cut(s)	1914
Bsp1720I	GCTNAGC	1 cut(s)	1334
BspACI	CCGC	8 cut(s)	55, 75, 123, 287, 429, 1220, 1396, 1669
BspANI	GGCC	5 cut(s)	37, 99, 1036, 1515, 1604
BspCNI	CTCAG	3 cut(s)	1135, 1347, 1884
BspDI	ATCGAT	1 cut(s)	2150
BspHI	TCATGA	1 cut(s)	102
BspLI	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 838, 878, 1822
BspMAI	CTGCAG	1 cut(s)	1773
BspMI	ACCTGC	1 cut(s)	1861
BspPI	GGATC	5 cut(s)	35, 669, 678, 1288, 1805
BspQI	GCTCTTC	1 cut(s)	104
BspT107I	GGYRCC	1 cut(s)	876
BsrFI	RCCGGY	1 cut(s)	590
BsrGI	TGTACA	1 cut(s)	1914
BsrI	ACTGG	7 cut(s)	262, 750, 837, 878, 1368, 1560, 1860
BssAI	RCCGGY	1 cut(s)	590
BssECI	CCNNGG	4 cut(s)	510, 543, 1031, 1265
BssNAI	GTATAC	1 cut(s)	367
BssNI	GRCGYC	4 cut(s)	78, 126, 877, 1294
BssSI	CACGAG	2 cut(s)	1166, 1632
BssT1I	CCWWGG	2 cut(s)	510, 1031
Bst1107I	GTATAC	1 cut(s)	367
Bst2BI	CACGAG	2 cut(s)	1166, 1632
Bst2UI	CCWGG	4 cut(s)	49, 1266, 1825, 2110
Bst4CI	ACNGT	3 cut(s)	491, 1175, 1405
Bst6I	CTCTTC	2 cut(s)	104, 1338
BstACI	GRCGYC	4 cut(s)	78, 126, 877, 1294
BstAPI	GCANNNNNTGC	1 cut(s)	993
BstAUI	TGTACA	1 cut(s)	1914
BstBAI	YACGTR	1 cut(s)	214
BstC8I	GCNNGC	4 cut(s)	180, 304, 928, 1593
BstDEI	CTNAG	3 cut(s)	1122, 1334, 1892
BstDSI	CCRYGG	1 cut(s)	543
BstEII	GGTNACC	1 cut(s)	676
BstF5I	GGATG	3 cut(s)	178, 1132, 1404
BstH2I	RGCGCY	1 cut(s)	880
BstHHI	GCGC	3 cut(s)	184, 879, 1930
BstMAI	GTCTC	1 cut(s)	1413
BstMCI	CGRYCG	1 cut(s)	291
BstNI	CCWGG	4 cut(s)	49, 1266, 1825, 2110
BstNSI	RCATGY	1 cut(s)	1309
BstPI	GGTNACC	1 cut(s)	676
BstSCI	CCNGG	6 cut(s)	47, 657, 1039, 1264, 1823, 2108
BstSFI	CTRYAG	1 cut(s)	1769
BstSLI	GKGCMC	1 cut(s)	1180
BstV1I	GCAGC	7 cut(s)	253, 443, 446, 694, 704, 1382, 1983
BstX2I	RGATCY	4 cut(s)	42, 670, 1280, 1921
BstYI	RGATCY	4 cut(s)	42, 670, 1280, 1921
BstZ17I	GTATAC	1 cut(s)	367
Bsu15I	ATCGAT	1 cut(s)	2150
BsuI	GTATCC	1 cut(s)	630
BsuRI	GGCC	5 cut(s)	37, 99, 1036, 1515, 1604
BsuTUI	ATCGAT	1 cut(s)	2150
BtgI	CCRYGG	1 cut(s)	543
BtsCI	GGATG	3 cut(s)	178, 1132, 1404
BtsIMutI	CAGTG	4 cut(s)	743, 1180, 1410, 1843
BveI	ACCTGC	1 cut(s)	1861
Cac8I	GCNNGC	4 cut(s)	180, 304, 928, 1593
CaiI	CAGNNNCTG	2 cut(s)	437, 1016
CciI	TCATGA	1 cut(s)	102
CfoI	GCGC	3 cut(s)	184, 879, 1930
Cfr10I	RCCGGY	1 cut(s)	590
Cfr13I	GGNCC	4 cut(s)	35, 51, 441, 575
ClaI	ATCGAT	1 cut(s)	2150
CseI	GACGC	4 cut(s)	67, 98, 115, 1283
CspAI	ACCGGT	1 cut(s)	590
CviAII	CATG	6 cut(s)	103, 993, 1306, 1465, 1706, 2023
DdeI	CTNAG	3 cut(s)	1122, 1334, 1892
DinI	GGCGCC	1 cut(s)	878
Eam1104I	CTCTTC	2 cut(s)	104, 1338
EarI	CTCTTC	2 cut(s)	104, 1338
Ecl136II	GAGCTC	3 cut(s)	66, 719, 2158
Eco130I	CCWWGG	2 cut(s)	510, 1031
Eco147I	AGGCCT	3 cut(s)	1036, 1515, 1604
Eco24I	GRGCYC	4 cut(s)	68, 87, 721, 2160
Eco47I	GGWCC	3 cut(s)	51, 441, 575
Eco53kI	GAGCTC	3 cut(s)	66, 719, 2158
Eco57I	CTGAAG	2 cut(s)	1020, 1983
Eco72I	CACGTG	1 cut(s)	214
Eco88I	CYCGRG	2 cut(s)	61, 2126
Eco91I	GGTNACC	1 cut(s)	676
EcoICRI	GAGCTC	3 cut(s)	66, 719, 2158
EcoO109I	RGGNCCY	1 cut(s)	575
EcoO65I	GGTNACC	1 cut(s)	676
EcoRI	GAATTC	1 cut(s)	1841
EcoRII	CCWGG	4 cut(s)	47, 1264, 1823, 2108
EcoT14I	CCWWGG	2 cut(s)	510, 1031
EcoT38I	GRGCYC	4 cut(s)	68, 87, 721, 2160
EgeI	GGCGCC	1 cut(s)	878
EheI	GGCGCC	1 cut(s)	878
ErhI	CCWWGG	2 cut(s)	510, 1031
FaeI	CATG	6 cut(s)	106, 996, 1309, 1468, 1709, 2026
FalI	AAGNNNNNCTT	2 cut(s)	215, 247
FaqI	GGGAC	3 cut(s)	37, 268, 1943
FatI	CATG	6 cut(s)	102, 992, 1305, 1464, 1705, 2022
FauI	CCCGC	2 cut(s)	62, 436
FauNDI	CATATG	3 cut(s)	346, 1288, 1354
FbaI	TGATCA	2 cut(s)	375, 1702
FblI	GTMKAC	2 cut(s)	366, 967
Fnu4HI	GCNGC	9 cut(s)	76, 124, 267, 432, 435, 693, 708, 1371, 1997
FokI	GGATG	3 cut(s)	185, 1139, 1411
FriOI	GRGCYC	4 cut(s)	68, 87, 721, 2160
Fsp4HI	GCNGC	9 cut(s)	76, 124, 267, 432, 435, 693, 708, 1371, 1997
FspBI	CTAG	3 cut(s)	557, 2051, 2169
GlaI	GCGC	3 cut(s)	183, 878, 1929
GluI	GCNGC	9 cut(s)	76, 124, 267, 432, 435, 693, 708, 1371, 1997
GsaI	CCCAGC	1 cut(s)	441
GsuI	CTGGAG	2 cut(s)	647, 717
HaeII	RGCGCY	1 cut(s)	880
HaeIII	GGCC	5 cut(s)	37, 99, 1036, 1515, 1604
HapII	CCGG	4 cut(s)	444, 591, 658, 1040
HgaI	GACGC	4 cut(s)	67, 98, 115, 1283
HhaI	GCGC	3 cut(s)	184, 879, 1930
Hin1I	GRCGYC	4 cut(s)	78, 126, 877, 1294
Hin1II	CATG	6 cut(s)	106, 996, 1309, 1468, 1709, 2026
Hin6I	GCGC	3 cut(s)	182, 877, 1928
HinP1I	GCGC	3 cut(s)	182, 877, 1928
HincII	GTYRAC	2 cut(s)	1318, 2048
HindII	GTYRAC	2 cut(s)	1318, 2048
HinfI	GANTC	5 cut(s)	10, 904, 1013, 1559, 2141
HpaI	GTTAAC	1 cut(s)	1318
HpaII	CCGG	4 cut(s)	444, 591, 658, 1040
HphI	GGTGA	4 cut(s)	1213, 1508, 1630, 1996
Hpy166II	GTNNAC	6 cut(s)	367, 494, 830, 968, 1318, 2048
Hpy188I	TCNGA	4 cut(s)	70, 506, 1893, 1900
Hpy8I	GTNNAC	6 cut(s)	367, 494, 830, 968, 1318, 2048
Hpy99I	CGWCG	5 cut(s)	83, 92, 119, 131, 669
HpyAV	CCTTC	3 cut(s)	1505, 1636, 2107
HpyCH4III	ACNGT	3 cut(s)	491, 1175, 1405
HpyCH4IV	ACGT	2 cut(s)	213, 1046
HpyF3I	CTNAG	3 cut(s)	1122, 1334, 1892
HpySE526I	ACGT	2 cut(s)	213, 1046
Hsp92I	GRCGYC	4 cut(s)	78, 126, 877, 1294
Hsp92II	CATG	6 cut(s)	106, 996, 1309, 1468, 1709, 2026
HspAI	GCGC	3 cut(s)	182, 877, 1928
KasI	GGCGCC	1 cut(s)	876
Ksp22I	TGATCA	2 cut(s)	375, 1702
KspAI	GTTAAC	1 cut(s)	1318
LguI	GCTCTTC	1 cut(s)	104
LmnI	GCTCC	9 cut(s)	531, 545, 724, 836, 1367, 1671, 1820, 2155, 2163
Lsp1109I	GCAGC	7 cut(s)	253, 443, 446, 694, 704, 1382, 1983
LweI	GCATC	6 cut(s)	997, 1120, 1347, 1489, 1578, 2156
MaeI	CTAG	3 cut(s)	557, 2051, 2169
MaeII	ACGT	2 cut(s)	213, 1046
MaeIII	GTNAC	8 cut(s)	412, 653, 676, 754, 1225, 1751, 2071, 2171
MfeI	CAATTG	1 cut(s)	2162
MflI	RGATCY	4 cut(s)	42, 670, 1280, 1921
MhlI	GDGCHC	5 cut(s)	68, 87, 721, 1180, 2160
Mly113I	GGCGCC	1 cut(s)	877
MmeI	TCCRAC	1 cut(s)	496
MroXI	GAANNNNTTC	1 cut(s)	227
MseI	TTAA	9 cut(s)	279, 383, 648, 846, 977, 1317, 1538, 1641, 2057
MslI	CAYNNNNRTG	3 cut(s)	591, 897, 1310
MspA1I	CMGCKG	3 cut(s)	437, 695, 1250
MspI	CCGG	4 cut(s)	444, 591, 658, 1040
MspR9I	CCNGG	6 cut(s)	49, 659, 1041, 1266, 1825, 2110
MunI	CAATTG	1 cut(s)	2162
Mva1269I	GAATGC	2 cut(s)	1031, 1545
MvaI	CCWGG	4 cut(s)	49, 1266, 1825, 2110
NarI	GGCGCC	1 cut(s)	877
NciI	CCSGG	2 cut(s)	659, 1041
NdeI	CATATG	3 cut(s)	346, 1288, 1354
NlaIII	CATG	6 cut(s)	106, 996, 1309, 1468, 1709, 2026
NlaIV	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 838, 878, 1822
NmuCI	GTSAC	3 cut(s)	653, 1225, 1751
NspI	RCATGY	1 cut(s)	1309
PagI	TCATGA	1 cut(s)	102
PceI	AGGCCT	3 cut(s)	1036, 1515, 1604
PciSI	GCTCTTC	1 cut(s)	104
PcsI	WCGNNNNNNNCGW	1 cut(s)	123
PctI	GAATGC	2 cut(s)	1031, 1545
PdmI	GAANNNNTTC	1 cut(s)	227
PfeI	GAWTC	5 cut(s)	10, 904, 1013, 1559, 2141
PflFI	GACNNNGTC	2 cut(s)	92, 1405
PfoI	TCCNGGA	1 cut(s)	1039
PinAI	ACCGGT	1 cut(s)	590
PkrI	GCNGC	9 cut(s)	77, 125, 268, 433, 436, 694, 709, 1372, 1998
PluTI	GGCGCC	1 cut(s)	880
PmaCI	CACGTG	1 cut(s)	214
PmlI	CACGTG	1 cut(s)	214
Ppu21I	YACGTR	1 cut(s)	214
PpuMI	RGGWCCY	1 cut(s)	575
PshBI	ATTAAT	1 cut(s)	279
Psp124BI	GAGCTC	3 cut(s)	68, 721, 2160
Psp5II	RGGWCCY	1 cut(s)	575
Psp6I	CCWGG	4 cut(s)	47, 1264, 1823, 2108
PspCI	CACGTG	1 cut(s)	214
PspEI	GGTNACC	1 cut(s)	676
PspFI	CCCAGC	1 cut(s)	437
PspGI	CCWGG	4 cut(s)	47, 1264, 1823, 2108
PspN4I	GGNNCC	8 cut(s)	36, 53, 442, 541, 576, 838, 878, 1822
PspPI	GGNCC	4 cut(s)	35, 51, 441, 575
PspPPI	RGGWCCY	1 cut(s)	575
PstI	CTGCAG	1 cut(s)	1773
PstNI	CAGNNNCTG	2 cut(s)	437, 1016
PsuI	RGATCY	4 cut(s)	42, 670, 1280, 1921
PsyI	GACNNNGTC	2 cut(s)	92, 1405
PvuII	CAGCTG	3 cut(s)	437, 695, 1250
RseI	CAYNNNNRTG	3 cut(s)	591, 897, 1310
SacI	GAGCTC	3 cut(s)	68, 721, 2160
SapI	GCTCTTC	1 cut(s)	104
SaqAI	TTAA	9 cut(s)	279, 383, 648, 846, 977, 1317, 1538, 1641, 2057
SatI	GCNGC	9 cut(s)	76, 124, 267, 432, 435, 693, 708, 1371, 1997
Sau96I	GGNCC	4 cut(s)	35, 51, 441, 575
ScrFI	CCNGG	6 cut(s)	49, 659, 1041, 1266, 1825, 2110
SduI	GDGCHC	5 cut(s)	68, 87, 721, 1180, 2160
SfaNI	GCATC	6 cut(s)	997, 1120, 1347, 1489, 1578, 2156
SfcI	CTRYAG	1 cut(s)	1769
SfoI	GGCGCC	1 cut(s)	878
SinI	GGWCC	3 cut(s)	51, 441, 575
SmiMI	CAYNNNNRTG	3 cut(s)	591, 897, 1310
SpeI	ACTAGT	1 cut(s)	2168
SseBI	AGGCCT	3 cut(s)	1036, 1515, 1604
SsiI	CCGC	8 cut(s)	55, 75, 123, 287, 429, 1220, 1396, 1669
SspDI	GGCGCC	1 cut(s)	876
SspI	AATATT	1 cut(s)	283
SspMI	CTAG	3 cut(s)	557, 2051, 2169
SstI	GAGCTC	3 cut(s)	68, 721, 2160
StuI	AGGCCT	3 cut(s)	1036, 1515, 1604
StyD4I	CCNGG	6 cut(s)	47, 657, 1039, 1264, 1823, 2108
StyI	CCWWGG	2 cut(s)	510, 1031
TaaI	ACNGT	3 cut(s)	491, 1175, 1405
TaiI	ACGT	2 cut(s)	216, 1049
TatI	WGTACW	5 cut(s)	493, 829, 1089, 1170, 1914
TauI	GCSGC	2 cut(s)	78, 126
TfiI	GAWTC	5 cut(s)	10, 904, 1013, 1559, 2141
Tru1I	TTAA	9 cut(s)	279, 383, 648, 846, 977, 1317, 1538, 1641, 2057
Tru9I	TTAA	9 cut(s)	279, 383, 648, 846, 977, 1317, 1538, 1641, 2057
TscAI	CASTG	4 cut(s)	750, 1180, 1410, 1843
TseFI	GTSAC	3 cut(s)	653, 1225, 1751
TseI	GCWGC	7 cut(s)	266, 431, 434, 692, 707, 1370, 1996
Tsp45I	GTSAC	3 cut(s)	653, 1225, 1751
TspDTI	ATGAA	6 cut(s)	119, 159, 265, 575, 1203, 2011
TspGWI	ACGGA	2 cut(s)	532, 2033
TspRI	CASTG	4 cut(s)	750, 1180, 1410, 1843
Tth111I	GACNNNGTC	2 cut(s)	92, 1405
VpaK11BI	GGWCC	3 cut(s)	51, 441, 575
VspI	ATTAAT	1 cut(s)	279
XapI	RAATTY	4 cut(s)	642, 1063, 1141, 1841
XceI	RCATGY	1 cut(s)	1309
XmiI	GTMKAC	2 cut(s)	366, 967
XmnI	GAANNNNTTC	1 cut(s)	227
XspI	CTAG	3 cut(s)	557, 2051, 2169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.