MD13G1241400.v1.1

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007221
Physical Location & Seq
Forward (+)
25100386 .. 25105368
4983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1241400.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGTTAAACTTCTTTGTGAGGAAAGGCGTTAGGAAGATTCTCAAGCGCAAGGACAGTGATGCTGGTGAAAGAGGAAGAGCTCTAGAAGACTTAAGATCTACCCTTTTTAATGAATTCCGCTCAGCCGATGGTGCAAAGCGTCAACAACAACAGCGATGTGGCCCTGCCACTGCTCTTACCTTCAATTTTGTAATTGCTGTTGGCATAATTTTTGTGAACAAATTGGTGCTCAAAGTTGTTGGGTTCCAGTTTCCTATATTTCTCACCTTTATCCACTATGTAGTAAGCTGGTTGTTGATGGCCATTCTAAATGCTTTATCTATCCTCCCTGCATGTCCCTCAAAATCAACCCGCTTATCTACATTATTTACTCTTGGCTTTGTTATGGCTTTCTCTACTGGCCTTGCTAATGTTAGCTTGAAGTATAATAGTGTGGGGTTCTATCAGATGTCTAAGATTGCGGTTACGCCATCAATTGTTCTCGCAGAATTTTTGTTGTACAACAAGAGAGTTTCTTTCTCGAAGGTTCTAGCACTTATGGTAGTATCTATTGGTGTTGCCGTGGCTACAGTCACTGATCTTCAATTTCATCTCTTTGGTGCTTGTATAGCATTAGCATGGATAATACCAAGTGCAGTCAACAAAATTCTTTGGTCTAGCTTGCAACAGCAGGAAAATTGGACAGCCTTAGCGTTAATGTGGAAGACAACACCAATCACTCTATTTTTCCTTGTCGCACTAATACCCTGGTTAGATCCACCTGGTGCCCTCTCGTTCAATTGGAACTTCAACAACACCTTGGCAATTCTCATGTCCGCTATACTTGGCTTCTTGCTTCAGTGGTCAGGTGCTCTAGCGCTTGGGGCTACATCTGCTGTCACACATGTCGTCCTTGGTCAATTTAAAACATGCGTCATTCTTCTAGGAAACTACTACCTCTTTAGTTCCAATCCAGGCAAGACAAGTATTTCTGGGGCTTTCACAGCCATTGCCGGAATGTCCATTTACACTTACCTTAATTTGAAGCTGCAATCAAGCAAAACTTCCCCTCGGCAAGCCACCTCTTTACCAAAATCTAAACTGAGCAAAGAAAACGGCAACACCCACGATGGAAATTATGGTTTGGAATCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

379

Amino Acids

41.48

Weight (kDa)

9.96

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 60 - 336 9.6e-16 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 4 cut(s) 118, 352, 461, 816
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 113, 488, 645
AcuI CTGAAG 1 cut(s) 821
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 1 cut(s) 500
AfeI AGCGCT 1 cut(s) 858
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 6 cut(s) 184, 421, 584, 778, 790, 1024
AjnI CCWGG 3 cut(s) 746, 760, 952
AluBI AGCT 5 cut(s) 80, 288, 417, 660, 1027
AluI AGCT 5 cut(s) 80, 288, 417, 660, 1027
Alw21I GWGCWC 3 cut(s) 82, 231, 853
AlwI GGATC 1 cut(s) 749
AlwNI CAGNNNCTG 1 cut(s) 575
Aor51HI AGCGCT 1 cut(s) 858
AoxI GGCC 3 cut(s) 160, 300, 400
ApeKI GCWGC 1 cut(s) 1027
ApoI RAATTY 3 cut(s) 113, 488, 645
AspLEI GCGC 2 cut(s) 48, 859
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 77, 256
BaeGI GKGCMC 1 cut(s) 769
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 2 cut(s) 93, 710
Bbv12I GWGCWC 3 cut(s) 82, 231, 853
BbvI GCAGC 1 cut(s) 1014
BccI CCATC 4 cut(s) 122, 292, 478, 1103
BceAI ACGGC 2 cut(s) 545, 1111
BciT130I CCWGG 3 cut(s) 748, 762, 954
BfaI CTAG 5 cut(s) 83, 530, 657, 854, 923
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 860
BfrI CTTAAG 1 cut(s) 91
BglII AGATCT 1 cut(s) 95
BisI GCNGC 1 cut(s) 1028
BlpI GCTNAGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 1029
Bme1390I CCNGG 3 cut(s) 748, 762, 954
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 245, 766
BmrFI CCNGG 3 cut(s) 748, 762, 954
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 2 cut(s) 93, 710
Bpu10I CCTNAGC 1 cut(s) 688
Bpu1102I GCTNAGC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 561, 746, 798, 892, 1049
Bse1I ACTGG 2 cut(s) 247, 403
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 3 cut(s) 748, 762, 954
BseDI CCNNGG 5 cut(s) 561, 746, 798, 892, 1049
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 2 cut(s) 135, 1073
BseNI ACTGG 2 cut(s) 247, 403
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 1 cut(s) 1014
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 3 cut(s) 162, 302, 402
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 3 cut(s) 82, 231, 853
BsiSI CCGG 1 cut(s) 993
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 3 cut(s) 162, 302, 402
Bsp1286I GDGCHC 4 cut(s) 82, 231, 769, 853
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 95, 577, 754
Bsp1720I GCTNAGC 1 cut(s) 121
BspACI CCGC 4 cut(s) 118, 352, 461, 816
BspANI GGCC 3 cut(s) 162, 302, 402
BspCNI CTCAG 2 cut(s) 134, 1074
BspLI GGNNCC 2 cut(s) 245, 766
BspPI GGATC 1 cut(s) 749
BspQI GCTCTTC 1 cut(s) 70
BspT107I GGYRCC 1 cut(s) 764
BspTI CTTAAG 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 987
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 247, 403
BssECI CCNNGG 5 cut(s) 561, 746, 798, 892, 1049
BssMI GATC 3 cut(s) 95, 577, 754
BssT1I CCWWGG 2 cut(s) 798, 892
Bst2UI CCWGG 3 cut(s) 748, 762, 954
Bst4CI ACNGT 2 cut(s) 56, 571
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 91
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 662, 1056
BstDEI CTNAG 4 cut(s) 121, 453, 688, 1082
BstDSI CCRYGG 1 cut(s) 561
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 2 cut(s) 48, 859
BstKTI GATC 3 cut(s) 98, 580, 757
BstMBI GATC 3 cut(s) 95, 577, 754
BstMWI GCNNNNNNNGC 5 cut(s) 131, 608, 863, 872, 983
BstNI CCWGG 3 cut(s) 748, 762, 954
BstNSI RCATGY 3 cut(s) 336, 887, 912
BstSCI CCNGG 3 cut(s) 746, 760, 952
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 769
BstV1I GCAGC 1 cut(s) 1014
BstV2I GAAGAC 2 cut(s) 93, 710
BstX2I RGATCY 2 cut(s) 95, 754
BstYI RGATCY 2 cut(s) 95, 754
BsuRI GGCC 3 cut(s) 162, 302, 402
BtgI CCRYGG 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 61, 168, 573, 845
Cac8I GCNNGC 2 cut(s) 662, 1056
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 2 cut(s) 48, 859
Cfr13I GGNCC 1 cut(s) 161
CseI GACGC 2 cut(s) 128, 901
CsiI ACCWGGT 1 cut(s) 760
Csp6I GTAC 1 cut(s) 499
CviAII CATG 5 cut(s) 333, 618, 811, 884, 909
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 4 cut(s) 121, 453, 688, 1082
DpnI GATC 3 cut(s) 97, 579, 756
DpnII GATC 3 cut(s) 95, 577, 754
DraI TTTAAA 1 cut(s) 904
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 798, 892
Eco24I GRGCYC 1 cut(s) 82
Eco47III AGCGCT 1 cut(s) 858
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 821
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 746, 760, 952
EcoT14I CCWWGG 2 cut(s) 798, 892
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 798, 892
FaeI CATG 5 cut(s) 336, 621, 814, 887, 912
FalI AAGNNNNNCTT 2 cut(s) 1027, 1059
FaqI GGGAC 1 cut(s) 321
FatI CATG 5 cut(s) 332, 617, 810, 883, 908
FauI CCCGC 1 cut(s) 359
Fnu4HI GCNGC 1 cut(s) 1028
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 1 cut(s) 1028
FspBI CTAG 5 cut(s) 83, 530, 657, 854, 923
GlaI GCGC 2 cut(s) 47, 858
GluI GCNGC 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 3 cut(s) 162, 302, 402
HapII CCGG 1 cut(s) 993
HgaI GACGC 2 cut(s) 128, 901
HhaI GCGC 2 cut(s) 48, 859
Hin1II CATG 5 cut(s) 336, 621, 814, 887, 912
Hin6I GCGC 2 cut(s) 46, 857
HinP1I GCGC 2 cut(s) 46, 857
HincII GTYRAC 2 cut(s) 143, 640
HindII GTYRAC 2 cut(s) 143, 640
HinfI GANTC 2 cut(s) 37, 1127
HpaII CCGG 1 cut(s) 993
HphI GGTGA 2 cut(s) 77, 256
Hpy166II GTNNAC 3 cut(s) 143, 217, 640
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 2 cut(s) 83, 520
Hpy8I GTNNAC 3 cut(s) 143, 217, 640
HpyAV CCTTC 2 cut(s) 190, 517
HpyCH4III ACNGT 2 cut(s) 56, 571
HpyCH4V TGCA 5 cut(s) 134, 332, 635, 664, 1030
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 608, 863, 872, 983
HpyF3I CTNAG 4 cut(s) 121, 453, 688, 1082
Hsp92II CATG 5 cut(s) 336, 621, 814, 887, 912
HspAI GCGC 2 cut(s) 46, 857
Kzo9I GATC 3 cut(s) 95, 577, 754
LguI GCTCTTC 1 cut(s) 70
Lsp1109I GCAGC 1 cut(s) 1014
LweI GCATC 1 cut(s) 49
MabI ACCWGGT 1 cut(s) 760
MaeI CTAG 5 cut(s) 83, 530, 657, 854, 923
MaeIII GTNAC 3 cut(s) 463, 571, 877
MalI GATC 3 cut(s) 97, 579, 756
MbiI CCGCTC 1 cut(s) 120
MboI GATC 3 cut(s) 95, 577, 754
MboII GAAGA 6 cut(s) 46, 87, 98, 572, 715, 911
MfeI CAATTG 2 cut(s) 474, 778
MflI RGATCY 2 cut(s) 95, 754
MhlI GDGCHC 4 cut(s) 82, 231, 769, 853
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 8 cut(s) 12, 65, 335, 349, 779, 947, 1059, 1072
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 6 cut(s) 5, 92, 108, 695, 903, 1017
MslI CAYNNNNRTG 1 cut(s) 616
Msp20I TGGCCA 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 1 cut(s) 993
MspR9I CCNGG 3 cut(s) 748, 762, 954
MunI CAATTG 2 cut(s) 474, 778
MvaI CCWGG 3 cut(s) 748, 762, 954
MwoI GCNNNNNNNGC 5 cut(s) 131, 608, 863, 872, 983
NdeII GATC 3 cut(s) 95, 577, 754
NlaIII CATG 5 cut(s) 336, 621, 814, 887, 912
NlaIV GGNNCC 2 cut(s) 245, 766
NmeAIII GCCGAG 1 cut(s) 1030
NmuCI GTSAC 2 cut(s) 571, 877
NspI RCATGY 3 cut(s) 336, 887, 912
PciI ACATGT 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 37, 1127
PkrI GCNGC 1 cut(s) 1029
PscI ACATGT 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 3 cut(s) 746, 760, 952
PspGI CCWGG 3 cut(s) 746, 760, 952
PspN4I GGNNCC 2 cut(s) 245, 766
PspPI GGNCC 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 2 cut(s) 95, 754
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 616
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 6 cut(s) 5, 92, 108, 695, 903, 1017
SatI GCNGC 1 cut(s) 1028
Sau3AI GATC 3 cut(s) 95, 577, 754
Sau96I GGNCC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 748, 762, 954
SduI GDGCHC 4 cut(s) 82, 231, 769, 853
SexAI ACCWGGT 1 cut(s) 760
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 41, 91
SmoI CTYRAG 2 cut(s) 41, 91
SsiI CCGC 4 cut(s) 118, 352, 461, 816
SspMI CTAG 5 cut(s) 83, 530, 657, 854, 923
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 3 cut(s) 746, 760, 952
StyI CCWWGG 2 cut(s) 798, 892
TaaI ACNGT 2 cut(s) 56, 571
TaqI TCGA 1 cut(s) 521
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 2 cut(s) 37, 1127
Tru1I TTAA 6 cut(s) 5, 92, 108, 695, 903, 1017
Tru9I TTAA 6 cut(s) 5, 92, 108, 695, 903, 1017
TscAI CASTG 4 cut(s) 61, 175, 580, 845
TseFI GTSAC 2 cut(s) 571, 877
TseI GCWGC 1 cut(s) 1027
Tsp45I GTSAC 2 cut(s) 571, 877
TspDTI ATGAA 2 cut(s) 126, 578
TspRI CASTG 4 cut(s) 61, 175, 580, 845
Vha464I CTTAAG 1 cut(s) 91
XapI RAATTY 3 cut(s) 113, 488, 645
XbaI TCTAGA 1 cut(s) 82
XceI RCATGY 3 cut(s) 336, 887, 912
XspI CTAG 5 cut(s) 83, 530, 657, 854, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.