MD16G1244800.v1.1

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007221
Physical Location & Seq
Reverse (-)
26632503 .. 26638059
5557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1244800.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGTTAAATTTCTTTCTAAGGAAAGGCGTTAGGAAGATTCTCAAGCGCAAGGATAGTGATGCTGGTGAAAGAGGAAGAGCTCTAGAAGACTTGCGATCTGTTCTTTTTAATGAATTCCGCTCATCCGATGGTGCAAGGCGTCAACAACAACAGCTATGTGGCCCTGCCACTGCTCTTACCTTCAATTTTGTAATTGCCGTTGGCATAATTTTCGTGAACAAATTGGTGCTCAAAGTTGTTGGGTTCCAGTTTCCTATATTTCTCACCTTTATCCACTATGTAGTAAGCTGGTTATTAATGGCCCTTCTAAATGCTCTTTGTATCCTCTCTGCATGTCCCTCAAAATCGACCCGCTCATCTACATTATTTACTCTTGGCTTTGTCATGGCTTTCTCTACTGGCCTTGCAAATGTTAGCTTGAAGTATAATAGTGTGGGGTTTTATCAGATGTCTAAAATTGCGGTTACGCCATCAATTGTTCTCGCAGAATTTTTGTTGTACAACAAGAGAGTTTCTTTCTCGAAGGTTCTAGCACTTACAGTAGTATCGATTGGTGTTGCCGTCGCTACAGTAACTGATCTGCAATTTCATCTCTTTGGTGCTTGTATAGCATTAGCATGGATAATACCAAGTGCAGTCAACAAAATTCTTTGGTCTAGCTTGCAACAGCAGGAAAATTGGACAGCCTTAGCATTAATGTGGAAGACAACGCCAATCACTCTATTTTTCCTTGTCGCACTAATCCCTTGGTTAGATCCACCTGGTGCCCTATCTTTCAATTGGAACTTCAACAACACCTTGGCAATTCTCATGTCAGCTATTCTTGGCTTCTTGCTTCAGTGGTCAGGCGCTCTAGCACTTGGGGCAACATCTGCTGTCACACATGTCGTTCTTGGTCAATTTAAAACATGCGTCATTCTTCTAGGAAACTATTACCTCTTTAGTTCCAATCCAGGCAAGACAAGCATTTCTGGGGCTTTCACAGCCATTGCCGGAATGTCCATTTACACTTACCTTAATTTGAAGCTGCAATCAAACAAAACTTTCCCTCGGCAAGCGTCTTCTTTACCAAAATCTAAAGAAAACAGCTACACCCACGTTGGAAATTATGGTTCGGAATCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

376

Amino Acids

41.23

Weight (kDa)

9.88

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 60 - 336 1.5e-14 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccBSI CCGCTC 2 cut(s) 120, 354
AciI CCGC 3 cut(s) 118, 352, 461
AclWI GGATC 1 cut(s) 749
AcsI RAATTY 4 cut(s) 7, 113, 488, 645
AcuI CTGAAG 1 cut(s) 821
AcyI GRCGYC 1 cut(s) 139
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 1 cut(s) 500
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 5 cut(s) 184, 421, 778, 790, 1024
AjnI CCWGG 2 cut(s) 760, 952
AluBI AGCT 8 cut(s) 80, 154, 288, 417, 660, 818, 1027, 1089
AluI AGCT 8 cut(s) 80, 154, 288, 417, 660, 818, 1027, 1089
Alw21I GWGCWC 2 cut(s) 82, 231
AlwI GGATC 1 cut(s) 749
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 3 cut(s) 160, 300, 400
ApeKI GCWGC 1 cut(s) 1027
ApoI RAATTY 4 cut(s) 7, 113, 488, 645
AseI ATTAAT 2 cut(s) 296, 695
AspLEI GCGC 2 cut(s) 48, 851
AspS9I GGNCC 2 cut(s) 161, 301
AsuHPI GGTGA 2 cut(s) 77, 256
BaeGI GKGCMC 1 cut(s) 769
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 3 cut(s) 93, 710, 1053
Bbv12I GWGCWC 2 cut(s) 82, 231
BbvI GCAGC 1 cut(s) 1014
BccI CCATC 2 cut(s) 122, 478
BceAI ACGGC 2 cut(s) 182, 545
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 2 cut(s) 762, 954
BciVI GTATCC 1 cut(s) 332
BfaI CTAG 5 cut(s) 83, 530, 657, 854, 923
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 852
BfuI GTATCC 1 cut(s) 332
BisI GCNGC 1 cut(s) 1028
BlsI GCNGC 1 cut(s) 1029
Bme1390I CCNGG 2 cut(s) 762, 954
BmgT120I GGNCC 2 cut(s) 161, 301
BmiI GGNNCC 2 cut(s) 245, 766
BmrFI CCNGG 2 cut(s) 762, 954
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 3 cut(s) 93, 710, 1053
Bpu10I CCTNAGC 1 cut(s) 688
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 746, 798, 1049
Bse1I ACTGG 2 cut(s) 247, 403
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 2 cut(s) 762, 954
BseCI ATCGAT 1 cut(s) 548
BseDI CCNNGG 3 cut(s) 746, 798, 1049
BseGI GGATG 1 cut(s) 122
BseMI GCAATG 1 cut(s) 987
BseNI ACTGG 2 cut(s) 247, 403
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 1 cut(s) 1014
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 3 cut(s) 162, 302, 402
BshNI GGYRCC 1 cut(s) 764
BshVI ATCGAT 1 cut(s) 548
BsiHKAI GWGCWC 2 cut(s) 82, 231
BsiSI CCGG 1 cut(s) 993
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 3 cut(s) 162, 302, 402
Bsp1286I GDGCHC 3 cut(s) 82, 231, 769
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 95, 577, 754
BspACI CCGC 3 cut(s) 118, 352, 461
BspANI GGCC 3 cut(s) 162, 302, 402
BspDI ATCGAT 1 cut(s) 548
BspLI GGNNCC 2 cut(s) 245, 766
BspPI GGATC 1 cut(s) 749
BspQI GCTCTTC 1 cut(s) 70
BspT107I GGYRCC 1 cut(s) 764
BsrBI CCGCTC 2 cut(s) 120, 354
BsrDI GCAATG 1 cut(s) 987
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 247, 403
BssECI CCNNGG 3 cut(s) 746, 798, 1049
BssMI GATC 3 cut(s) 95, 577, 754
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 746, 798
Bst2UI CCWGG 2 cut(s) 762, 954
Bst4CI ACNGT 2 cut(s) 541, 571
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 139
BstAPI GCANNNNNTGC 1 cut(s) 872
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 662, 1056
BstDEI CTNAG 2 cut(s) 17, 688
BstF5I GGATG 1 cut(s) 122
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 2 cut(s) 48, 851
BstKTI GATC 3 cut(s) 98, 580, 757
BstMBI GATC 3 cut(s) 95, 577, 754
BstMWI GCNNNNNNNGC 5 cut(s) 608, 863, 872, 963, 983
BstNI CCWGG 2 cut(s) 762, 954
BstNSI RCATGY 3 cut(s) 336, 887, 912
BstSCI CCNGG 2 cut(s) 760, 952
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 769
BstV1I GCAGC 1 cut(s) 1014
BstV2I GAAGAC 3 cut(s) 93, 710, 1053
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
Bsu15I ATCGAT 1 cut(s) 548
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 3 cut(s) 162, 302, 402
BsuTUI ATCGAT 1 cut(s) 548
BtsCI GGATG 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 168, 845
Cac8I GCNNGC 2 cut(s) 662, 1056
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 2 cut(s) 48, 851
Cfr13I GGNCC 2 cut(s) 161, 301
ClaI ATCGAT 1 cut(s) 548
CseI GACGC 3 cut(s) 128, 901, 1047
CsiI ACCWGGT 1 cut(s) 760
Csp6I GTAC 1 cut(s) 499
CviAII CATG 6 cut(s) 333, 385, 618, 811, 884, 909
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 2 cut(s) 17, 688
DpnI GATC 3 cut(s) 97, 579, 756
DpnII GATC 3 cut(s) 95, 577, 754
DraI TTTAAA 1 cut(s) 904
DraIII CACNNNGTG 1 cut(s) 764
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 746, 798
Eco24I GRGCYC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 821
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 760, 952
EcoT14I CCWWGG 2 cut(s) 746, 798
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 746, 798
FaeI CATG 6 cut(s) 336, 388, 621, 814, 887, 912
FaqI GGGAC 1 cut(s) 321
FatI CATG 6 cut(s) 332, 384, 617, 810, 883, 908
FauI CCCGC 1 cut(s) 359
Fnu4HI GCNGC 1 cut(s) 1028
FokI GGATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 1 cut(s) 1028
FspBI CTAG 5 cut(s) 83, 530, 657, 854, 923
GlaI GCGC 2 cut(s) 47, 850
GluI GCNGC 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 3 cut(s) 162, 302, 402
HapII CCGG 1 cut(s) 993
HgaI GACGC 3 cut(s) 128, 901, 1047
HhaI GCGC 2 cut(s) 48, 851
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 6 cut(s) 336, 388, 621, 814, 887, 912
Hin6I GCGC 2 cut(s) 46, 849
HinP1I GCGC 2 cut(s) 46, 849
HincII GTYRAC 2 cut(s) 143, 640
HindII GTYRAC 2 cut(s) 143, 640
HinfI GANTC 2 cut(s) 37, 1118
HpaII CCGG 1 cut(s) 993
HphI GGTGA 2 cut(s) 77, 256
Hpy166II GTNNAC 3 cut(s) 143, 217, 640
Hpy188I TCNGA 3 cut(s) 127, 447, 1117
Hpy188III TCNNGA 3 cut(s) 83, 214, 520
Hpy8I GTNNAC 3 cut(s) 143, 217, 640
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 190, 314, 517
HpyCH4III ACNGT 2 cut(s) 541, 571
HpyCH4IV ACGT 1 cut(s) 1098
HpyCH4V TGCA 7 cut(s) 134, 332, 407, 583, 635, 664, 1030
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 863, 872, 963, 983
HpyF3I CTNAG 2 cut(s) 17, 688
HpySE526I ACGT 1 cut(s) 1098
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 6 cut(s) 336, 388, 621, 814, 887, 912
HspAI GCGC 2 cut(s) 46, 849
Kzo9I GATC 3 cut(s) 95, 577, 754
LguI GCTCTTC 1 cut(s) 70
Lsp1109I GCAGC 1 cut(s) 1014
LweI GCATC 1 cut(s) 49
MabI ACCWGGT 1 cut(s) 760
MaeI CTAG 5 cut(s) 83, 530, 657, 854, 923
MaeII ACGT 1 cut(s) 1098
MaeIII GTNAC 3 cut(s) 463, 571, 877
MalI GATC 3 cut(s) 97, 579, 756
MbiI CCGCTC 2 cut(s) 120, 354
MboI GATC 3 cut(s) 95, 577, 754
MboII GAAGA 6 cut(s) 46, 87, 98, 715, 911, 1053
MfeI CAATTG 2 cut(s) 474, 778
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 3 cut(s) 82, 231, 769
MmeI TCCRAC 1 cut(s) 1081
MnlI CCTC 5 cut(s) 65, 335, 349, 947, 1059
MseI TTAA 6 cut(s) 5, 108, 296, 695, 903, 1017
MslI CAYNNNNRTG 2 cut(s) 616, 697
MspI CCGG 1 cut(s) 993
MspR9I CCNGG 2 cut(s) 762, 954
MunI CAATTG 2 cut(s) 474, 778
MvaI CCWGG 2 cut(s) 762, 954
MwoI GCNNNNNNNGC 5 cut(s) 608, 863, 872, 963, 983
NdeII GATC 3 cut(s) 95, 577, 754
NlaIII CATG 6 cut(s) 336, 388, 621, 814, 887, 912
NlaIV GGNNCC 2 cut(s) 245, 766
NmeAIII GCCGAG 1 cut(s) 1030
NmuCI GTSAC 1 cut(s) 877
NspI RCATGY 3 cut(s) 336, 887, 912
PciI ACATGT 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 37, 1118
PkrI GCNGC 1 cut(s) 1029
PscI ACATGT 1 cut(s) 883
PshBI ATTAAT 2 cut(s) 296, 695
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 760, 952
PspGI CCWGG 2 cut(s) 760, 952
PspN4I GGNNCC 2 cut(s) 245, 766
PspPI GGNCC 2 cut(s) 161, 301
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 1 cut(s) 754
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 2 cut(s) 616, 697
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 6 cut(s) 5, 108, 296, 695, 903, 1017
SatI GCNGC 1 cut(s) 1028
Sau3AI GATC 3 cut(s) 95, 577, 754
Sau96I GGNCC 2 cut(s) 161, 301
ScrFI CCNGG 2 cut(s) 762, 954
SduI GDGCHC 3 cut(s) 82, 231, 769
SexAI ACCWGGT 1 cut(s) 760
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 567
SmiMI CAYNNNNRTG 2 cut(s) 616, 697
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SsiI CCGC 3 cut(s) 118, 352, 461
SspMI CTAG 5 cut(s) 83, 530, 657, 854, 923
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 760, 952
StyI CCWWGG 2 cut(s) 746, 798
TaaI ACNGT 2 cut(s) 541, 571
TaiI ACGT 1 cut(s) 1101
TaqI TCGA 3 cut(s) 347, 521, 548
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 2 cut(s) 37, 1118
Tru1I TTAA 6 cut(s) 5, 108, 296, 695, 903, 1017
Tru9I TTAA 6 cut(s) 5, 108, 296, 695, 903, 1017
TscAI CASTG 2 cut(s) 175, 845
TseFI GTSAC 1 cut(s) 877
TseI GCWGC 1 cut(s) 1027
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 2 cut(s) 126, 578
TspRI CASTG 2 cut(s) 175, 845
VspI ATTAAT 2 cut(s) 296, 695
XapI RAATTY 4 cut(s) 7, 113, 488, 645
XbaI TCTAGA 1 cut(s) 82
XceI RCATGY 3 cut(s) 336, 887, 912
XspI CTAG 5 cut(s) 83, 530, 657, 854, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.