MD13G1242200.v1.1

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012345
Physical Location & Seq
Forward (+)
25214013 .. 25222692
8680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1242200.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGAGCTTTCGGAAGGCCTATGGAGCTCTGAAGGACTCTACCAAGGTTGGCCTCATCAAGGTCAACAGCGAATTCAAGGATTTGGATATCACCACTGTGAAGGCAACCAGTCACGTTGAGTGCCCTCCGAAGGAACGTCATATTCGAAAGATTTTCTCTGCGACATCAGTGAATCGTCCACGCGCAGATGTGGCATACTGCATTCACGCGTTGGCCAAGAGATTGTCCAAGACACGGAGTTGGATTGTTGCCATAAAGACATTGATAGTTATTCATAGAACATTGAGAGATGGTGATCCTACCTTCAGAGAGGAGCTTCTGAACTATTCACAGAGAGGACACATTCTCCAAATTTCCTATTTTAAAGATGACTCAAGTCCCCTTGCTTGGGATTGCTCCGCTTGGGTTAGGACATATGCGCTCTTTTTAGAAGAACGGCTGGAGTGTTTCAAAGTTTTGAAATATGACATTGAGGCAGAGCGTTTGACCAAAACATTGCCAGGATCAACCAAGGTCCATAGTAGAACACGAACGCTGAGTGCTGATGCGCTCTTGGAGCAGCTACCTGCACTGCAGCAACTTCTTTTCTGCCTCATGGGTTGCCAGCCCGAGGGAACAGCTTATAACAATTACCTCATACAATATGCCTTGGCTCTGATATTAAAAGAGAGCTTTAAAATATATTGTGCCATCAATGACGGAATTATAAATCTCGTTGACATGTTCTTTGATATGTCAAGACACGACGCAGTTAAAGCCCTCGATATTTACAAAAGAGCTGGTCAACAGGCTGAAGCGCTTGCTGATTTTTACGAGTGCTGCAAGGGTTTGGATCTTGCTAGAACTTTTCAGTTTCCAACATTGAGACAGCCACCACCATCATTTCTTGCAACAATGGAAGAGTATATAAAGGAAGCGCCAGAGTCAGGTTCCGTTAACAGAAGACTGGAATACCAGGAGACAGATGAGCAGCCTCAGAGACCTGAAGAACCTCCTCCCCCTCCAGAGCCTGAAAAACAAGAAGAAAAAGTTGAGGAGCCACTGGCTAAAACAGAGGAAGAACCCCAACCCAAGGAAGAAGAAGTCGAACCTCCTCCTTTGATATCAACTGAGGACATTGATCTGCTGGGTCTAAGGGAAATAAATCCAAAAGCTGCAGAAATAGAAGAATGCAATGCCCTGGCTCTTGCGATAGTTCCACAGGGGAATGAACAAAAATCTGGAGCAAGTTTCAATGATTTTGCTGGGACTTCCGGTTGGGAGCTAGCGCTGGTGACCACACCAAGCAACCATACCAGCCAAGCGCCCCAAGACAGAAAGTTGGCTGGTGGCTTTGATAATCTGTTACTTAATAGTTTGTATGAAGATGAAGGTGCCAGGAGACAGTTACAACTCCAGAATGCAGGGTATGGTTATGGCACGACGAGCTTGCAAAATCCATTTGAACAAGCAGCACAGCAACCCGTACAAGACCCATTTGCAATGTCCAACAGCATAGCACCCCCTACAAACGTGCAGATGGCGTTAATGGCACAACAACAACACCAGCAAATCATGCAGCAACAACAACAGCAACACCAACAACAGCAAAACATGATGATGGTACCTCACCCGTATTATTCTCAATATTCCCAACCAATGCAATCCGCAGGCTCTGCAAACCCGTTTGGAGATCCATTCAGCATCCCTCCTAGTACAATCCCAAACCAGGGAAACCAAAACCTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

577

Amino Acids

64.96

Weight (kDa)

5.22

Isoelectric Point (pI)

61.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 27 - 305 3.2e-91 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25940 AT5G57200
fragaria_vesca FvH4_4g07320
malus_domestica MD13G1242200.v1.1 MD16G1249100.v1.1
prunus_persica Prupe.1G075800_v2.0.a1 Prupe.1G076000_v2.0.a1
pyrus_communis pycom13g21390 pycom16g20850
rosa_chinensis RchiOBHm_Chr4g0399681
rosa_laevigata RLG00000009247
rosa_multiflora Rmu_co8405013.1_g000001
rosa_roxburghii Rroxscaffold_5G00344590
rosa_rugosa Rorug04G0018100
rosa_samantha Rh4AG095900 Rh4BG093400 Rh4CG104500 Rh4DG088500
rosa_wichuraiana Rw4G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 624, 707
Acc36I ACCTGC 1 cut(s) 574
Acc65I GGTACC 1 cut(s) 1603
AccB1I GGYRCC 2 cut(s) 1373, 1603
AccII CGCG 2 cut(s) 183, 209
AciI CCGC 2 cut(s) 399, 1647
AclWI GGATC 4 cut(s) 290, 511, 840, 1667
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 2 cut(s) 71, 351
AcuI CTGAAG 4 cut(s) 50, 289, 813, 1005
AfaI GTAC 3 cut(s) 1467, 1605, 1696
AfeI AGCGCT 2 cut(s) 798, 1269
AflIII ACRYGT 2 cut(s) 207, 720
AgsI TTSAA 5 cut(s) 76, 451, 460, 1234, 1445
AjnI CCWGG 5 cut(s) 499, 954, 1179, 1376, 1707
AleI CACNNNNGTG 1 cut(s) 95
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 4 cut(s) 859, 953, 973, 1375
AlwI GGATC 4 cut(s) 290, 511, 840, 1667
AlwNI CAGNNNCTG 2 cut(s) 1010, 1655
Ama87I CYCGRG 1 cut(s) 608
Aor51HI AGCGCT 2 cut(s) 798, 1269
AoxI GGCC 3 cut(s) 15, 49, 213
ApeKI GCWGC 7 cut(s) 559, 574, 819, 970, 1154, 1451, 1558
ApoI RAATTY 2 cut(s) 71, 351
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
Asp718I GGTACC 1 cut(s) 1603
AspLEI GCGC 7 cut(s) 185, 421, 550, 799, 919, 1270, 1306
AspS9I GGNCC 1 cut(s) 514
AsuHPI GGTGA 4 cut(s) 82, 305, 1285, 1601
AsuII TTCGAA 1 cut(s) 145
AsuNHI GCTAGC 1 cut(s) 1264
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 125
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 2 cut(s) 1373, 1603
BanII GRGCYC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 949
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 7 cut(s) 571, 586, 806, 982, 1141, 1463, 1570
BccI CCATC 5 cut(s) 284, 698, 886, 1513, 1594
BceAI ACGGC 1 cut(s) 452
BcgI CGANNNNNNTGC 2 cut(s) 1411, 1445
BciT130I CCWGG 5 cut(s) 501, 956, 1181, 1378, 1709
BcoDI GTCTC 4 cut(s) 859, 953, 973, 1375
BfaI CTAG 3 cut(s) 840, 1265, 1692
BfmI CTRYAG 2 cut(s) 572, 1155
BfoI RGCGCY 4 cut(s) 800, 920, 1271, 1307
BfuAI ACCTGC 1 cut(s) 574
BisI GCNGC 7 cut(s) 560, 575, 820, 971, 1155, 1452, 1559
BlsI GCNGC 7 cut(s) 561, 576, 821, 972, 1156, 1453, 1560
Bme1390I CCNGG 5 cut(s) 501, 956, 1181, 1378, 1709
Bme18I GGWCC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 4 cut(s) 931, 1038, 1375, 1605
BmrFI CCNGG 5 cut(s) 501, 956, 1181, 1378, 1709
BmsI GCATC 2 cut(s) 535, 1692
BmtI GCTAGC 1 cut(s) 1268
BoxI GACNNNNGTC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 949
BpmI CTGGAG 4 cut(s) 461, 987, 1242, 1379
Bpu14I TTCGAA 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 358
BsaBI GATNNNNATC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 973
BsaJI CCNNGG 7 cut(s) 42, 510, 609, 648, 1071, 1179, 1708
BsaWI WCCGGW 1 cut(s) 1253
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bse1I ACTGG 3 cut(s) 108, 951, 1047
Bse3DI GCAATG 3 cut(s) 494, 1180, 1488
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 5 cut(s) 501, 956, 1181, 1378, 1709
BseDI CCNNGG 7 cut(s) 42, 510, 609, 648, 1071, 1179, 1708
BseGI GGATG 1 cut(s) 1683
BseJI GATNNNNATC 1 cut(s) 294
BseMI GCAATG 3 cut(s) 494, 1180, 1488
BseMII CTCAG 3 cut(s) 527, 989, 1101
BseNI ACTGG 3 cut(s) 108, 951, 1047
BseRI GAGGAG 4 cut(s) 326, 984, 1049, 1083
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 7 cut(s) 571, 586, 806, 982, 1141, 1463, 1570
BseYI CCCAGC 2 cut(s) 1126, 1244
BsgI GTGCAG 2 cut(s) 552, 1535
Bsh1236I CGCG 2 cut(s) 183, 209
BshFI GGCC 3 cut(s) 17, 51, 215
BshNI GGYRCC 2 cut(s) 1373, 1603
BsiHKAI GWGCWC 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 1 cut(s) 1254
BslFI GGGAC 2 cut(s) 363, 1261
BsmAI GTCTC 4 cut(s) 859, 953, 973, 1375
BsmFI GGGAC 2 cut(s) 363, 1261
BsmI GAATGC 3 cut(s) 201, 1175, 1405
BsnI GGCC 3 cut(s) 17, 51, 215
Bso31I GGTCTC 1 cut(s) 973
BsoBI CYCGRG 1 cut(s) 608
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 28, 125
Bsp143I GATC 5 cut(s) 295, 503, 832, 1120, 1672
BspACI CCGC 2 cut(s) 399, 1647
BspANI GGCC 3 cut(s) 17, 51, 215
BspCNI CTCAG 3 cut(s) 528, 988, 1102
BspFNI CGCG 2 cut(s) 183, 209
BspLI GGNNCC 4 cut(s) 931, 1038, 1375, 1605
BspMAI CTGCAG 2 cut(s) 576, 1159
BspMI ACCTGC 1 cut(s) 574
BspOI GCTAGC 1 cut(s) 1268
BspPI GGATC 4 cut(s) 290, 511, 840, 1667
BspT104I TTCGAA 1 cut(s) 145
BspT107I GGYRCC 2 cut(s) 1373, 1603
BspTNI GGTCTC 1 cut(s) 973
BsrDI GCAATG 3 cut(s) 494, 1180, 1488
BsrI ACTGG 3 cut(s) 108, 951, 1047
BssECI CCNNGG 7 cut(s) 42, 510, 609, 648, 1071, 1179, 1708
BssMI GATC 5 cut(s) 295, 503, 832, 1120, 1672
BssT1I CCWWGG 4 cut(s) 42, 510, 648, 1071
Bst2UI CCWGG 5 cut(s) 501, 956, 1181, 1378, 1709
Bst4CI ACNGT 2 cut(s) 97, 1386
Bst6I CTCTTC 1 cut(s) 894
BstAPI GCANNNNNTGC 2 cut(s) 1555, 1655
BstBI TTCGAA 1 cut(s) 145
BstC8I GCNNGC 5 cut(s) 605, 801, 1266, 1430, 1651
BstDEI CTNAG 4 cut(s) 536, 975, 1110, 1133
BstEII GGTNACC 1 cut(s) 1273
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 1683
BstFNI CGCG 2 cut(s) 183, 209
BstH2I RGCGCY 4 cut(s) 800, 920, 1271, 1307
BstHHI GCGC 7 cut(s) 185, 421, 550, 799, 919, 1270, 1306
BstKTI GATC 5 cut(s) 298, 506, 835, 1123, 1675
BstMAI GTCTC 4 cut(s) 859, 953, 973, 1375
BstMBI GATC 5 cut(s) 295, 503, 832, 1120, 1672
BstMWI GCNNNNNNNGC 8 cut(s) 23, 191, 556, 755, 1425, 1529, 1555, 1655
BstNI CCWGG 5 cut(s) 501, 956, 1181, 1378, 1709
BstNSI RCATGY 1 cut(s) 724
BstPAI GACNNNNGTC 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 1273
BstSCI CCNGG 5 cut(s) 499, 954, 1179, 1376, 1707
BstSFI CTRYAG 2 cut(s) 572, 1155
BstSLI GKGCMC 1 cut(s) 125
BstUI CGCG 2 cut(s) 183, 209
BstV1I GCAGC 7 cut(s) 571, 586, 806, 982, 1141, 1463, 1570
BstV2I GAAGAC 1 cut(s) 949
BstX2I RGATCY 2 cut(s) 832, 1672
BstYI RGATCY 2 cut(s) 832, 1672
BsuRI GGCC 3 cut(s) 17, 51, 215
BtsCI GGATG 1 cut(s) 1683
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 4 cut(s) 93, 174, 569, 1040
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 5 cut(s) 605, 801, 1266, 1430, 1651
CaiI CAGNNNCTG 2 cut(s) 1010, 1655
CfoI GCGC 7 cut(s) 185, 421, 550, 799, 919, 1270, 1306
Cfr13I GGNCC 1 cut(s) 514
CseI GACGC 1 cut(s) 755
Csp6I GTAC 3 cut(s) 1466, 1604, 1695
CviAII CATG 4 cut(s) 595, 721, 1555, 1594
CviQI GTAC 3 cut(s) 1466, 1604, 1695
DdeI CTNAG 4 cut(s) 536, 975, 1110, 1133
DpnI GATC 5 cut(s) 297, 505, 834, 1122, 1674
DpnII GATC 5 cut(s) 295, 503, 832, 1120, 1672
DraI TTTAAA 2 cut(s) 364, 676
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 4 cut(s) 42, 510, 648, 1071
Eco147I AGGCCT 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 973
Eco32I GATATC 2 cut(s) 88, 1104
Eco47I GGWCC 1 cut(s) 514
Eco47III AGCGCT 2 cut(s) 798, 1269
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 4 cut(s) 50, 289, 813, 1005
Eco88I CYCGRG 1 cut(s) 608
Eco91I GGTNACC 1 cut(s) 1273
EcoICRI GAGCTC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO65I GGTNACC 1 cut(s) 1273
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 5 cut(s) 499, 954, 1179, 1376, 1707
EcoRV GATATC 2 cut(s) 88, 1104
EcoT14I CCWWGG 4 cut(s) 42, 510, 648, 1071
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 4 cut(s) 42, 510, 648, 1071
FaeI CATG 4 cut(s) 598, 724, 1558, 1597
FaqI GGGAC 2 cut(s) 363, 1261
FatI CATG 4 cut(s) 594, 720, 1554, 1593
FauNDI CATATG 1 cut(s) 415
Fnu4HI GCNGC 7 cut(s) 560, 575, 820, 971, 1155, 1452, 1559
FokI GGATG 1 cut(s) 1670
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 7 cut(s) 560, 575, 820, 971, 1155, 1452, 1559
FspBI CTAG 3 cut(s) 840, 1265, 1692
GlaI GCGC 7 cut(s) 184, 420, 549, 798, 918, 1269, 1305
GluI GCNGC 7 cut(s) 560, 575, 820, 971, 1155, 1452, 1559
GsaI CCCAGC 2 cut(s) 1130, 1248
GsuI CTGGAG 4 cut(s) 461, 987, 1242, 1379
HaeII RGCGCY 4 cut(s) 800, 920, 1271, 1307
HaeIII GGCC 3 cut(s) 17, 51, 215
HapII CCGG 1 cut(s) 1254
HgaI GACGC 1 cut(s) 755
HhaI GCGC 7 cut(s) 185, 421, 550, 799, 919, 1270, 1306
Hin1II CATG 4 cut(s) 598, 724, 1558, 1597
Hin6I GCGC 7 cut(s) 183, 419, 548, 797, 917, 1268, 1304
HinP1I GCGC 7 cut(s) 183, 419, 548, 797, 917, 1268, 1304
HincII GTYRAC 4 cut(s) 64, 718, 785, 937
HindII GTYRAC 4 cut(s) 64, 718, 785, 937
HinfI GANTC 4 cut(s) 35, 172, 371, 923
HpaI GTTAAC 1 cut(s) 937
HpaII CCGG 1 cut(s) 1254
HphI GGTGA 4 cut(s) 82, 305, 1285, 1601
Hpy166II GTNNAC 5 cut(s) 64, 179, 718, 785, 937
Hpy188I TCNGA 7 cut(s) 12, 30, 129, 308, 321, 657, 978
Hpy188III TCNNGA 4 cut(s) 738, 1004, 1221, 1396
Hpy8I GTNNAC 5 cut(s) 64, 179, 718, 785, 937
Hpy99I CGWCG 2 cut(s) 749, 1426
HpyAV CCTTC 6 cut(s) 7, 25, 94, 124, 313, 1364
HpyCH4III ACNGT 2 cut(s) 97, 1386
HpyCH4IV ACGT 3 cut(s) 114, 136, 1512
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 191, 556, 755, 1425, 1529, 1555, 1655
HpyF3I CTNAG 4 cut(s) 536, 975, 1110, 1133
HpySE526I ACGT 3 cut(s) 114, 136, 1512
Hsp92II CATG 4 cut(s) 598, 724, 1558, 1597
HspAI GCGC 7 cut(s) 183, 419, 548, 797, 917, 1268, 1304
KpnI GGTACC 1 cut(s) 1607
KspAI GTTAAC 1 cut(s) 937
Kzo9I GATC 5 cut(s) 295, 503, 832, 1120, 1672
LmnI GCTCC 7 cut(s) 23, 313, 401, 556, 1036, 1223, 1261
Lsp1109I GCAGC 7 cut(s) 571, 586, 806, 982, 1141, 1463, 1570
LweI GCATC 2 cut(s) 535, 1692
MaeI CTAG 3 cut(s) 840, 1265, 1692
MaeII ACGT 3 cut(s) 114, 136, 1512
MaeIII GTNAC 4 cut(s) 110, 1273, 1344, 1386
MalI GATC 5 cut(s) 297, 505, 834, 1122, 1674
MboI GATC 5 cut(s) 295, 503, 832, 1120, 1672
MflI RGATCY 2 cut(s) 832, 1672
MhlI GDGCHC 2 cut(s) 28, 125
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 4 cut(s) 71, 351, 628, 702
MluI ACGCGT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 3 cut(s) 29, 365, 932
MmeI TCCRAC 3 cut(s) 221, 881, 1512
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 7 cut(s) 363, 662, 675, 753, 936, 1350, 1526
MslI CAYNNNNRTG 2 cut(s) 95, 1598
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 1 cut(s) 1254
MspR9I CCNGG 5 cut(s) 501, 956, 1181, 1378, 1709
Mva1269I GAATGC 3 cut(s) 201, 1175, 1405
MvaI CCWGG 5 cut(s) 501, 956, 1181, 1378, 1709
MvnI CGCG 2 cut(s) 183, 209
MwoI GCNNNNNNNGC 8 cut(s) 23, 191, 556, 755, 1425, 1529, 1555, 1655
NdeI CATATG 1 cut(s) 415
NdeII GATC 5 cut(s) 295, 503, 832, 1120, 1672
NheI GCTAGC 1 cut(s) 1264
NlaIII CATG 4 cut(s) 598, 724, 1558, 1597
NlaIV GGNNCC 4 cut(s) 931, 1038, 1375, 1605
NmuCI GTSAC 2 cut(s) 110, 1273
NspI RCATGY 1 cut(s) 724
NspV TTCGAA 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 95
PceI AGGCCT 1 cut(s) 17
PciI ACATGT 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PctI GAATGC 3 cut(s) 201, 1175, 1405
PfeI GAWTC 1 cut(s) 172
PkrI GCNGC 7 cut(s) 561, 576, 821, 972, 1156, 1453, 1560
PleI GAGTC 3 cut(s) 29, 365, 931
PpsI GAGTC 3 cut(s) 29, 365, 931
PscI ACATGT 1 cut(s) 720
PshAI GACNNNNGTC 1 cut(s) 375
PsiI TTATAA 2 cut(s) 624, 707
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 5 cut(s) 499, 954, 1179, 1376, 1707
PspEI GGTNACC 1 cut(s) 1273
PspFI CCCAGC 2 cut(s) 1126, 1244
PspGI CCWGG 5 cut(s) 499, 954, 1179, 1376, 1707
PspN4I GGNNCC 4 cut(s) 931, 1038, 1375, 1605
PspPI GGNCC 1 cut(s) 514
PstI CTGCAG 2 cut(s) 576, 1159
PstNI CAGNNNCTG 2 cut(s) 1010, 1655
PsuI RGATCY 2 cut(s) 832, 1672
RsaI GTAC 3 cut(s) 1467, 1605, 1696
RsaNI GTAC 3 cut(s) 1466, 1604, 1695
RseI CAYNNNNRTG 2 cut(s) 95, 1598
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 7 cut(s) 363, 662, 675, 753, 936, 1350, 1526
SatI GCNGC 7 cut(s) 560, 575, 820, 971, 1155, 1452, 1559
Sau3AI GATC 5 cut(s) 295, 503, 832, 1120, 1672
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 3 cut(s) 29, 365, 932
ScrFI CCNGG 5 cut(s) 501, 956, 1181, 1378, 1709
SduI GDGCHC 2 cut(s) 28, 125
SfaNI GCATC 2 cut(s) 535, 1692
SfcI CTRYAG 2 cut(s) 572, 1155
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 2 cut(s) 95, 1598
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 4 cut(s) 71, 351, 628, 702
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 2 cut(s) 399, 1647
SspI AATATT 1 cut(s) 1628
SspMI CTAG 3 cut(s) 840, 1265, 1692
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 5 cut(s) 499, 954, 1179, 1376, 1707
StyI CCWWGG 4 cut(s) 42, 510, 648, 1071
TaaI ACNGT 2 cut(s) 97, 1386
TaiI ACGT 3 cut(s) 117, 139, 1515
TaqI TCGA 3 cut(s) 145, 762, 1086
TasI AATT 4 cut(s) 71, 351, 628, 702
TatI WGTACW 1 cut(s) 1694
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 7 cut(s) 363, 662, 675, 753, 936, 1350, 1526
Tru9I TTAA 7 cut(s) 363, 662, 675, 753, 936, 1350, 1526
TscAI CASTG 4 cut(s) 100, 174, 576, 1047
TseFI GTSAC 2 cut(s) 110, 1273
TseI GCWGC 7 cut(s) 559, 574, 819, 970, 1154, 1451, 1558
Tsp45I GTSAC 2 cut(s) 110, 1273
TspDTI ATGAA 4 cut(s) 263, 1224, 1377, 1383
TspGWI ACGGA 3 cut(s) 250, 714, 922
TspRI CASTG 4 cut(s) 100, 174, 576, 1047
VpaK11BI GGWCC 1 cut(s) 514
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 2 cut(s) 71, 351
XceI RCATGY 1 cut(s) 724
XspI CTAG 3 cut(s) 840, 1265, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.