Rorug04G0018100

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012345
Physical Location & Seq
Reverse (-)
2670043 .. 2670862
820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0018100.1

Sequence Viewer

Length: 594 bp
ATGGCTGCTTCAGCTACCATGCAATCAATATTCCTTGCAAATTCCGTTGTATATGGAGCAGGAAAGAACAGGTCCTCGTTGCCTGTTCAATTTGCACCAACTCTTCGTAGGTACCGTAACATGAGGGTACGCTCCATGGCTGAGAATGGTCTACCTGAGGAGAAGCCATCTACAGTTTCAGAACCATCAAAGGCTCCACCACAAGCACCTACACCTACACCTGTCCCACCACCACCTAGGACTCCCAAGGTTAGCACCAAGTTCGGGGACGTTTTTGCCTTCAGCGGTCCAGCACCGGAGAGAATCAACGGCAGACTCGCAATGGTGGGCTTTGTTTCAGCTCTGGCAGTGGAACTGTTAAAGGGCCAGGATGTGTTTTCTCAGATCTCCGACGGGGGAGTCTCGCTGTTTCTTGGCACTAGCATTTTGCTGTCAGTTGCATCCGTGATTCCTCTATTCCAAGGAGTCAGTGTGGAGTCGAAATCAAAGGGACTTATGACCTCAGATGCTGAGTTGTGGAATGGAAGATTGGCCATGTTAGGTTTGGTGGCTTTGGCTTTCACCGAGTATGTCAAGGGTGGAACCCTAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

197

Amino Acids

20.8

Weight (kDa)

9.84

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 98 - 189 1.6e-09 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25940 AT5G57200
fragaria_vesca FvH4_4g07320
malus_domestica MD13G1242200.v1.1 MD16G1249100.v1.1
prunus_persica Prupe.1G075800_v2.0.a1 Prupe.1G076000_v2.0.a1
pyrus_communis pycom13g21390 pycom16g20850
rosa_chinensis RchiOBHm_Chr4g0399681
rosa_laevigata RLG00000009247
rosa_multiflora Rmu_co8405013.1_g000001
rosa_roxburghii Rroxscaffold_5G00344590
rosa_rugosa Rorug04G0018100
rosa_samantha Rh4AG095900 Rh4BG093400 Rh4CG104500 Rh4DG088500
rosa_wichuraiana Rw4G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 151
AciI CCGC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 265
AfaI GTAC 2 cut(s) 113, 129
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 1 cut(s) 366
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 2 cut(s) 14, 341
AluI AGCT 2 cut(s) 14, 341
Alw26I GTCTC 1 cut(s) 406
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 2 cut(s) 364, 531
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 40
Asp718I GGTACC 1 cut(s) 111
AspA2I CCTAGG 1 cut(s) 236
AspS9I GGNCC 3 cut(s) 72, 287, 364
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 2 cut(s) 72, 287
AvrII CCTAGG 1 cut(s) 236
AxyI CCTNAGG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 533
BanI GGYRCC 1 cut(s) 111
BccI CCATC 2 cut(s) 175, 193
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 406
BfaI CTAG 2 cut(s) 237, 420
BfmI CTRYAG 1 cut(s) 171
BglII AGATCT 1 cut(s) 384
BisI GCNGC 1 cut(s) 6
BlnI CCTAGG 1 cut(s) 236
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 2 cut(s) 72, 287
BmgT120I GGNCC 3 cut(s) 72, 287, 364
BmiI GGNNCC 3 cut(s) 113, 195, 583
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 2 cut(s) 449, 496
BsaJI CCNNGG 4 cut(s) 135, 236, 246, 460
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse21I CCTNAGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 4 cut(s) 135, 236, 246, 460
BseGI GGATG 2 cut(s) 376, 440
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 5 cut(s) 132, 147, 395, 501, 516
BseRI GAGGAG 1 cut(s) 173
BshFI GGCC 2 cut(s) 366, 533
BshNI GGYRCC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 3 cut(s) 209, 281, 504
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 406
BsmFI GGGAC 3 cut(s) 209, 281, 504
BsnI GGCC 2 cut(s) 366, 533
Bsp143I GATC 1 cut(s) 384
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 1 cut(s) 285
BspANI GGCC 2 cut(s) 366, 533
BspCNI CTCAG 5 cut(s) 133, 148, 394, 502, 515
BspLI GGNNCC 3 cut(s) 113, 195, 583
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 327
BssECI CCNNGG 4 cut(s) 135, 236, 246, 460
BssMI GATC 1 cut(s) 384
BssT1I CCWWGG 4 cut(s) 135, 236, 246, 460
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 3 cut(s) 116, 175, 357
Bst6I CTCTTC 1 cut(s) 108
BstDEI CTNAG 5 cut(s) 141, 156, 381, 502, 510
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 376, 440
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 366, 533
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 2 cut(s) 376, 440
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 354, 475
CaiI CAGNNNCTG 1 cut(s) 509
Cfr13I GGNCC 3 cut(s) 72, 287, 364
Csp6I GTAC 2 cut(s) 112, 128
CviAII CATG 4 cut(s) 19, 121, 136, 535
CviQI GTAC 2 cut(s) 112, 128
DdeI CTNAG 5 cut(s) 141, 156, 381, 502, 510
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 4 cut(s) 135, 236, 246, 460
Eco47I GGWCC 2 cut(s) 72, 287
Eco57I CTGAAG 1 cut(s) 265
Eco81I CCTNAGG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 4 cut(s) 135, 236, 246, 460
ErhI CCWWGG 4 cut(s) 135, 236, 246, 460
FaeI CATG 4 cut(s) 22, 124, 139, 538
FaiI YATR 8 cut(s) 20, 52, 54, 122, 137, 497, 536, 570
FaqI GGGAC 3 cut(s) 209, 281, 504
FatI CATG 4 cut(s) 18, 120, 135, 534
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 383, 427
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 237, 420
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 366, 533
HapII CCGG 1 cut(s) 296
Hin1II CATG 4 cut(s) 22, 124, 139, 538
HinfI GANTC 7 cut(s) 241, 303, 315, 399, 448, 465, 476
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 4 cut(s) 181, 384, 391, 505
Hpy8I GTNNAC 1 cut(s) 152
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 3 cut(s) 116, 175, 357
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 4 cut(s) 22, 38, 95, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 5 cut(s) 141, 156, 381, 502, 510
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 4 cut(s) 22, 124, 139, 538
KpnI GGTACC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 3 cut(s) 56, 137, 199
LweI GCATC 2 cut(s) 449, 496
MaeI CTAG 2 cut(s) 237, 420
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 116
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 2 cut(s) 95, 537
MflI RGATCY 1 cut(s) 384
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 3 cut(s) 40, 89, 588
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 5 cut(s) 235, 309, 408, 474, 485
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 5 cut(s) 85, 117, 151, 462, 511
Mox20I TGGCCA 1 cut(s) 533
MscI TGGCCA 1 cut(s) 533
MseI TTAA 2 cut(s) 359, 592
Msp20I TGGCCA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 285
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 1 cut(s) 11
NcoI CCATGG 1 cut(s) 135
NdeII GATC 1 cut(s) 384
NlaIII CATG 4 cut(s) 22, 124, 139, 538
NlaIV GGNNCC 3 cut(s) 113, 195, 583
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PfeI GAWTC 2 cut(s) 303, 448
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 5 cut(s) 235, 309, 407, 473, 484
PpsI GAGTC 5 cut(s) 235, 309, 407, 473, 484
PpuMI RGGWCCY 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 3 cut(s) 113, 195, 583
PspPI GGNCC 3 cut(s) 72, 287, 364
PspPPI RGGWCCY 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 113, 129
RsaNI GTAC 2 cut(s) 112, 128
SaqAI TTAA 2 cut(s) 359, 592
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 3 cut(s) 72, 287, 364
SchI GAGTC 5 cut(s) 235, 309, 408, 474, 485
ScrFI CCNGG 1 cut(s) 368
SfaNI GCATC 2 cut(s) 449, 496
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 72, 287
Sse9I AATT 3 cut(s) 40, 89, 588
SsiI CCGC 1 cut(s) 285
SspI AATATT 1 cut(s) 30
SspMI CTAG 2 cut(s) 237, 420
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 4 cut(s) 135, 236, 246, 460
TaaI ACNGT 3 cut(s) 116, 175, 357
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 479
TasI AATT 3 cut(s) 40, 89, 588
TfiI GAWTC 2 cut(s) 303, 448
Tru1I TTAA 2 cut(s) 359, 592
Tru9I TTAA 2 cut(s) 359, 592
TscAI CASTG 2 cut(s) 354, 475
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 2 cut(s) 34, 433
TspRI CASTG 2 cut(s) 354, 475
VpaK11BI GGWCC 2 cut(s) 72, 287
XapI RAATTY 1 cut(s) 40
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmaJI CCTAGG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 151
XspI CTAG 2 cut(s) 237, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.