Rroxscaffold_5G00344590

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012345
Physical Location & Seq
Forward (+)
14734275 .. 14738334
4060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344590.1

Sequence Viewer

Length: 882 bp
ATGGTCTCTGACAATCTTCTTCTCTACGTTAAACAGCCACCGCCGTCATTTCTTGCAACAATGGAAGAGTACATAAAAGAAGCGCCTCAGTCCGGTGCTGTTCAGAAGAGACTGGAGTACCACGAGACAGAAGAGCGGCCTCAACAACCAGTGCCTCCGCCTCCAGAGCCTGAAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGTTGAGGAACCACCGGAAAAAGTTGAGGAAGAACCCCAACCAAAGGAGGAAGAGGGCCTAAATGAAATAAATCCAAGAGCTGCAGAAATTGAAGAAAGCAATGCTCTAGCTCTTGCTATAATTCCAAATGGGACTGAACCACAATCTGGATCAAATTTGAATGACATCGGTGGGACTTCAGGTTGGGAGCTGGCCCTGGTAACCACACCAAGCACCAATACTGGCCCCGTGGTAGATAAAAAATTGGCTGGTGGGTTTGACAAGCTATTACTCGATAGCTTGTATGAAGATGATGGTGCAAGGAGACAGCTACAACTCCAGAATGCAGGATATGGTTATGGCGCGACAAGCCTGCAAAATCCATTTGAACAAGCACAGCAGCAGCCTGTACATGATCCATTTGCAATGTCTAACAGCATAGCGCCTCCTACGAATGTGCAAATGGCACTAATGGCTCAACAACAACACCAAGTACACCAACAGCAACACCAAATGCAGCAACAGTATCAACAACAACAGAACATGATGATGGTACCTCACCCATATTATTCTCAATATCAACAGGCGCAACAACCCGCAGGCCCTTCGAATCCATTTGGAGACCCTTTCAGCTTCCCTCCTCCTTCTTCTAGTGCAATGCCTAACCAGGGAAACCAACACCTACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

293

Amino Acids

32.72

Weight (kDa)

4.33

Isoelectric Point (pI)

82.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25940 AT5G57200
fragaria_vesca FvH4_4g07320
malus_domestica MD13G1242200.v1.1 MD16G1249100.v1.1
prunus_persica Prupe.1G075800_v2.0.a1 Prupe.1G076000_v2.0.a1
pyrus_communis pycom13g21390 pycom16g20850
rosa_chinensis RchiOBHm_Chr4g0399681
rosa_laevigata RLG00000009247
rosa_multiflora Rmu_co8405013.1_g000001
rosa_roxburghii Rroxscaffold_5G00344590
rosa_rugosa Rorug04G0018100
rosa_samantha Rh4AG095900 Rh4BG093400 Rh4CG104500 Rh4DG088500
rosa_wichuraiana Rw4G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 745
AccB1I GGYRCC 1 cut(s) 745
AccB7I CCANNNNNTGG 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 1 cut(s) 557
AciI CCGC 4 cut(s) 41, 136, 158, 789
AclWI GGATC 2 cut(s) 370, 602
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 5 cut(s) 71, 119, 603, 687, 747
AfiI CCNNNNNNNGG 4 cut(s) 92, 256, 359, 860
AgsI TTSAA 3 cut(s) 305, 373, 581
AjnI CCWGG 2 cut(s) 408, 858
AluBI AGCT 7 cut(s) 293, 323, 403, 478, 492, 523, 825
AluI AGCT 7 cut(s) 293, 323, 403, 478, 492, 523, 825
Alw26I GTCTC 5 cut(s) 10, 103, 119, 511, 807
AlwI GGATC 2 cut(s) 370, 602
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 5 cut(s) 137, 268, 405, 436, 793
ApeKI GCWGC 4 cut(s) 293, 592, 595, 709
ApoI RAATTY 1 cut(s) 367
Asp718I GGTACC 1 cut(s) 745
AspLEI GCGC 4 cut(s) 85, 557, 637, 781
AspS9I GGNCC 4 cut(s) 268, 406, 437, 794
AsuHPI GGTGA 1 cut(s) 743
AsuII TTCGAA 1 cut(s) 800
BaeI ACNNNNGTAYC 2 cut(s) 101, 134
BanI GGYRCC 1 cut(s) 745
BauI CACGAG 1 cut(s) 122
BbvI GCAGC 4 cut(s) 280, 604, 607, 721
BccI CCATC 2 cut(s) 500, 736
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 4 cut(s) 547, 581, 780, 814
BciT130I CCWGG 2 cut(s) 410, 860
BcoDI GTCTC 5 cut(s) 10, 103, 119, 511, 807
BfaI CTAG 2 cut(s) 320, 843
BfmI CTRYAG 2 cut(s) 294, 878
BfoI RGCGCY 2 cut(s) 86, 638
BisI GCNGC 5 cut(s) 137, 294, 593, 596, 710
BlsI GCNGC 5 cut(s) 138, 295, 594, 597, 711
Bme1390I CCNGG 2 cut(s) 410, 860
BmgT120I GGNCC 4 cut(s) 268, 406, 437, 794
BmiI GGNNCC 3 cut(s) 222, 439, 747
BmrFI CCNGG 2 cut(s) 410, 860
BpmI CTGGAG 3 cut(s) 134, 147, 515
Bpu14I TTCGAA 1 cut(s) 800
BsaI GGTCTC 2 cut(s) 10, 807
BsaJI CCNNGG 3 cut(s) 408, 441, 859
BsaWI WCCGGW 2 cut(s) 92, 226
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 256, 359, 860
Bse1I ACTGG 3 cut(s) 117, 149, 439
Bse3DI GCAATG 3 cut(s) 319, 624, 855
BseBI CCWGG 2 cut(s) 410, 860
BseDI CCNNGG 3 cut(s) 408, 441, 859
BseLI CCNNNNNNNGG 4 cut(s) 92, 256, 359, 860
BseMI GCAATG 3 cut(s) 319, 624, 855
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 3 cut(s) 117, 149, 439
BseRI GAGGAG 1 cut(s) 822
BseXI GCAGC 4 cut(s) 280, 604, 607, 721
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 5 cut(s) 139, 270, 407, 438, 795
BshNI GGYRCC 1 cut(s) 745
BsiSI CCGG 2 cut(s) 93, 227
BslFI GGGAC 2 cut(s) 358, 400
BslI CCNNNNNNNGG 4 cut(s) 92, 256, 359, 860
BsmAI GTCTC 5 cut(s) 10, 103, 119, 511, 807
BsmFI GGGAC 2 cut(s) 358, 400
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 5 cut(s) 139, 270, 407, 438, 795
Bso31I GGTCTC 2 cut(s) 10, 807
Bsp119I TTCGAA 1 cut(s) 800
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 2 cut(s) 362, 607
BspACI CCGC 4 cut(s) 41, 136, 158, 789
BspANI GGCC 5 cut(s) 139, 270, 407, 438, 795
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 222, 439, 747
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 2 cut(s) 370, 602
BspQI GCTCTTC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 745
BspTNI GGTCTC 2 cut(s) 10, 807
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 3 cut(s) 319, 624, 855
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 3 cut(s) 117, 149, 439
BssECI CCNNGG 3 cut(s) 408, 441, 859
BssMI GATC 2 cut(s) 362, 607
BssSI CACGAG 1 cut(s) 122
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 410, 860
Bst4CI ACNGT 2 cut(s) 717, 879
Bst6I CTCTTC 4 cut(s) 60, 101, 126, 258
BstAUI TGTACA 1 cut(s) 601
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 3 cut(s) 405, 566, 793
BstDEI CTNAG 1 cut(s) 87
BstDSI CCRYGG 1 cut(s) 441
BstEII GGTNACC 1 cut(s) 412
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 2 cut(s) 86, 638
BstHHI GCGC 4 cut(s) 85, 557, 637, 781
BstKTI GATC 2 cut(s) 365, 610
BstMAI GTCTC 5 cut(s) 10, 103, 119, 511, 807
BstMBI GATC 2 cut(s) 362, 607
BstMWI GCNNNNNNNGC 3 cut(s) 166, 561, 665
BstNI CCWGG 2 cut(s) 410, 860
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 408, 858
BstSFI CTRYAG 2 cut(s) 294, 878
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 4 cut(s) 280, 604, 607, 721
BsuRI GGCC 5 cut(s) 139, 270, 407, 438, 795
BtgI CCRYGG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 405, 566, 793
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 4 cut(s) 85, 557, 637, 781
Cfr13I GGNCC 4 cut(s) 268, 406, 437, 794
Csp6I GTAC 5 cut(s) 70, 118, 602, 686, 746
CviAII CATG 2 cut(s) 605, 736
CviQI GTAC 5 cut(s) 70, 118, 602, 686, 746
DdeI CTNAG 1 cut(s) 87
DpnI GATC 2 cut(s) 364, 609
DpnII GATC 2 cut(s) 362, 607
Eam1104I CTCTTC 4 cut(s) 60, 101, 126, 258
EarI CTCTTC 4 cut(s) 60, 101, 126, 258
EciI GGCGGA 1 cut(s) 147
Eco31I GGTCTC 2 cut(s) 10, 807
Eco57I CTGAAG 1 cut(s) 375
Eco91I GGTNACC 1 cut(s) 412
EcoO109I RGGNCCY 2 cut(s) 268, 794
EcoO65I GGTNACC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 408, 858
FaeI CATG 2 cut(s) 608, 739
FaiI YATR 9 cut(s) 74, 332, 498, 546, 552, 606, 632, 737, 757
FaqI GGGAC 2 cut(s) 358, 400
FatI CATG 2 cut(s) 604, 735
FauI CCCGC 1 cut(s) 796
Fnu4HI GCNGC 5 cut(s) 137, 294, 593, 596, 710
Fsp4HI GCNGC 5 cut(s) 137, 294, 593, 596, 710
FspBI CTAG 2 cut(s) 320, 843
GlaI GCGC 4 cut(s) 84, 556, 636, 780
GluI GCNGC 5 cut(s) 137, 294, 593, 596, 710
GsuI CTGGAG 3 cut(s) 134, 147, 515
HaeII RGCGCY 2 cut(s) 86, 638
HaeIII GGCC 5 cut(s) 139, 270, 407, 438, 795
HapII CCGG 2 cut(s) 93, 227
HhaI GCGC 4 cut(s) 85, 557, 637, 781
Hin1II CATG 2 cut(s) 608, 739
Hin6I GCGC 4 cut(s) 83, 555, 635, 779
HinP1I GCGC 4 cut(s) 83, 555, 635, 779
HinfI GANTC 1 cut(s) 802
HpaII CCGG 2 cut(s) 93, 227
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 1 cut(s) 688
Hpy188I TCNGA 2 cut(s) 10, 105
Hpy188III TCNNGA 3 cut(s) 164, 360, 532
Hpy8I GTNNAC 1 cut(s) 688
HpyAV CCTTC 2 cut(s) 807, 846
HpyCH4III ACNGT 2 cut(s) 717, 879
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 9 cut(s) 56, 296, 512, 539, 568, 617, 652, 709, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 561, 665
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 608, 739
HspAI GCGC 4 cut(s) 83, 555, 635, 779
KpnI GGTACC 1 cut(s) 749
Kzo9I GATC 2 cut(s) 362, 607
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 4 cut(s) 280, 604, 607, 721
MaeI CTAG 2 cut(s) 320, 843
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 412
MalI GATC 2 cut(s) 364, 609
MbiI CCGCTC 1 cut(s) 136
MboI GATC 2 cut(s) 362, 607
MluCI AATT 4 cut(s) 300, 333, 367, 455
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 1 cut(s) 740
MspI CCGG 2 cut(s) 93, 227
MspR9I CCNGG 2 cut(s) 410, 860
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 410, 860
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 166, 561, 665
NdeII GATC 2 cut(s) 362, 607
NlaIII CATG 2 cut(s) 608, 739
NlaIV GGNNCC 3 cut(s) 222, 439, 747
NspV TTCGAA 1 cut(s) 800
PciSI GCTCTTC 1 cut(s) 126
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 802
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 5 cut(s) 138, 295, 594, 597, 711
Psp6I CCWGG 2 cut(s) 408, 858
PspEI GGTNACC 1 cut(s) 412
PspGI CCWGG 2 cut(s) 408, 858
PspN4I GGNNCC 3 cut(s) 222, 439, 747
PspPI GGNCC 4 cut(s) 268, 406, 437, 794
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 5 cut(s) 71, 119, 603, 687, 747
RsaNI GTAC 5 cut(s) 70, 118, 602, 686, 746
RseI CAYNNNNRTG 1 cut(s) 740
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 5 cut(s) 137, 294, 593, 596, 710
Sau3AI GATC 2 cut(s) 362, 607
Sau96I GGNCC 4 cut(s) 268, 406, 437, 794
ScrFI CCNGG 2 cut(s) 410, 860
SfcI CTRYAG 2 cut(s) 294, 878
SfuI TTCGAA 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 740
Sse9I AATT 4 cut(s) 300, 333, 367, 455
SsiI CCGC 4 cut(s) 41, 136, 158, 789
SspMI CTAG 2 cut(s) 320, 843
StyD4I CCNGG 2 cut(s) 408, 858
TaaI ACNGT 2 cut(s) 717, 879
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 486, 800
TasI AATT 4 cut(s) 300, 333, 367, 455
TatI WGTACW 3 cut(s) 69, 601, 685
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 1 cut(s) 802
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 1 cut(s) 156
TseI GCWGC 4 cut(s) 293, 592, 595, 709
TspDTI ATGAA 2 cut(s) 291, 513
TspRI CASTG 1 cut(s) 156
Van91I CCANNNNNTGG 1 cut(s) 359
XapI RAATTY 1 cut(s) 367
XspI CTAG 2 cut(s) 320, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.