The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 55 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 206 |
| Acc36I | ACCTGC | 1 cut(s) | 55 |
| AccBSI | CCGCTC | 1 cut(s) | 2311 |
| AccIII | TCCGGA | 1 cut(s) | 2766 |
| AciI | CCGC | 5 cut(s) | 770, 1233, 2311, 2661, 2757 |
| AclI | AACGTT | 1 cut(s) | 149 |
| AclWI | GGATC | 4 cut(s) | 558, 1608, 1621, 1632 |
| AcoI | YGGCCR | 1 cut(s) | 2312 |
| AcsI | RAATTY | 3 cut(s) | 298, 415, 2588 |
| AcuI | CTGAAG | 2 cut(s) | 216, 1416 |
| AfaI | GTAC | 3 cut(s) | 728, 1191, 2440 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1711 |
| AflII | CTTAAG | 1 cut(s) | 2297 |
| AjiI | CACGTC | 2 cut(s) | 91, 961 |
| AjnI | CCWGG | 2 cut(s) | 597, 2667 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2255, 2287 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1622, 1654 |
| Alw21I | GWGCWC | 1 cut(s) | 1184 |
| Alw26I | GTCTC | 7 cut(s) | 1209, 1783, 1952, 1986, 2246, 2378, 2411 |
| AlwI | GGATC | 4 cut(s) | 558, 1608, 1621, 1632 |
| Ama87I | CYCGRG | 1 cut(s) | 77 |
| Aor13HI | TCCGGA | 1 cut(s) | 2766 |
| AoxI | GGCC | 1 cut(s) | 2312 |
| ApeKI | GCWGC | 6 cut(s) | 833, 836, 856, 1571, 1786, 2672 |
| ApoI | RAATTY | 3 cut(s) | 298, 415, 2588 |
| AspLEI | GCGC | 1 cut(s) | 760 |
| AspS9I | GGNCC | 2 cut(s) | 1720, 2520 |
| AsuHPI | GGTGA | 9 cut(s) | 155, 665, 1127, 1667, 1892, 2221, 2303, 2666, 2728 |
| AvaI | CYCGRG | 1 cut(s) | 77 |
| AvaII | GGWCC | 2 cut(s) | 1720, 2520 |
| BaeGI | GKGCMC | 3 cut(s) | 598, 973, 1360 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1181, 1214 |
| BamHI | GGATCC | 1 cut(s) | 1613 |
| BanII | GRGCYC | 1 cut(s) | 393 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 710, 742, 1465, 1497 |
| BbsI | GAAGAC | 4 cut(s) | 204, 915, 1010, 1512 |
| Bbv12I | GWGCWC | 1 cut(s) | 1184 |
| BbvI | GCAGC | 6 cut(s) | 820, 823, 843, 1558, 1773, 2684 |
| BccI | CCATC | 8 cut(s) | 1195, 1541, 1602, 1735, 2501, 2645, 2647, 2738 |
| BceAI | ACGGC | 1 cut(s) | 1899 |
| BciT130I | CCWGG | 2 cut(s) | 599, 2669 |
| BclI | TGATCA | 1 cut(s) | 253 |
| BcoDI | GTCTC | 7 cut(s) | 1209, 1783, 1952, 1986, 2246, 2378, 2411 |
| BfaI | CTAG | 5 cut(s) | 978, 1010, 1103, 2330, 2343 |
| BfmI | CTRYAG | 3 cut(s) | 314, 540, 2097 |
| BfrI | CTTAAG | 1 cut(s) | 2297 |
| BfuAI | ACCTGC | 1 cut(s) | 55 |
| BisI | GCNGC | 8 cut(s) | 834, 837, 857, 1572, 1787, 2312, 2673, 2758 |
| BlpI | GCTNAGC | 1 cut(s) | 2753 |
| BlsI | GCNGC | 8 cut(s) | 835, 838, 858, 1573, 1788, 2313, 2674, 2759 |
| Bme1390I | CCNGG | 2 cut(s) | 599, 2669 |
| Bme18I | GGWCC | 2 cut(s) | 1720, 2520 |
| BmeT110I | CYCGRG | 1 cut(s) | 77 |
| BmgBI | CACGTC | 2 cut(s) | 91, 961 |
| BmgT120I | GGNCC | 2 cut(s) | 1720, 2520 |
| BmiI | GGNNCC | 1 cut(s) | 1615 |
| BmrFI | CCNGG | 2 cut(s) | 599, 2669 |
| BmrI | ACTGGG | 1 cut(s) | 2168 |
| BmuI | ACTGGG | 1 cut(s) | 2168 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2390 |
| BpiI | GAAGAC | 4 cut(s) | 204, 915, 1010, 1512 |
| BpmI | CTGGAG | 2 cut(s) | 1788, 2409 |
| Bpu10I | CCTNAGC | 2 cut(s) | 965, 1618 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2753 |
| BpuEI | CTTGAG | 2 cut(s) | 1654, 2807 |
| BsaI | GGTCTC | 2 cut(s) | 2246, 2411 |
| BsaJI | CCNNGG | 8 cut(s) | 597, 709, 1432, 1666, 2247, 2286, 2712, 2785 |
| BsaWI | WCCGGW | 1 cut(s) | 2766 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1662, 1692, 2094, 2124, 2317, 2347 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1711 |
| Bse1I | ACTGG | 8 cut(s) | 1805, 1882, 2061, 2067, 2164, 2174, 2392, 2652 |
| Bse3DI | GCAATG | 3 cut(s) | 91, 117, 1228 |
| BseAI | TCCGGA | 1 cut(s) | 2766 |
| BseBI | CCWGG | 2 cut(s) | 599, 2669 |
| BseDI | CCNNGG | 8 cut(s) | 597, 709, 1432, 1666, 2247, 2286, 2712, 2785 |
| BseGI | GGATG | 8 cut(s) | 100, 430, 1594, 1816, 2128, 2148, 2656, 2713 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1711 |
| BseMI | GCAATG | 3 cut(s) | 91, 117, 1228 |
| BseMII | CTCAG | 8 cut(s) | 881, 1103, 1467, 1577, 1663, 1697, 2373, 2812 |
| BseNI | ACTGG | 8 cut(s) | 1805, 1882, 2061, 2067, 2164, 2174, 2392, 2652 |
| BseSI | GKGCMC | 3 cut(s) | 598, 973, 1360 |
| BseXI | GCAGC | 6 cut(s) | 820, 823, 843, 1558, 1773, 2684 |
| BseYI | CCCAGC | 1 cut(s) | 788 |
| BsgI | GTGCAG | 2 cut(s) | 627, 2619 |
| BshFI | GGCC | 1 cut(s) | 2314 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1184 |
| BsiHKCI | CYCGRG | 1 cut(s) | 77 |
| BsiSI | CCGG | 1 cut(s) | 2767 |
| BslFI | GGGAC | 2 cut(s) | 55, 805 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1711 |
| BsmAI | GTCTC | 7 cut(s) | 1209, 1783, 1952, 1986, 2246, 2378, 2411 |
| BsmFI | GGGAC | 2 cut(s) | 55, 805 |
| BsmI | GAATGC | 2 cut(s) | 574, 760 |
| BsnI | GGCC | 1 cut(s) | 2314 |
| Bso31I | GGTCTC | 2 cut(s) | 2246, 2411 |
| BsoBI | CYCGRG | 1 cut(s) | 77 |
| Bsp1286I | GDGCHC | 5 cut(s) | 393, 598, 973, 1184, 1360 |
| Bsp13I | TCCGGA | 1 cut(s) | 2766 |
| Bsp143I | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2753 |
| Bsp19I | CCATGG | 1 cut(s) | 2286 |
| BspACI | CCGC | 5 cut(s) | 770, 1233, 2311, 2661, 2757 |
| BspANI | GGCC | 1 cut(s) | 2314 |
| BspCNI | CTCAG | 8 cut(s) | 880, 1102, 1466, 1576, 1662, 1698, 2374, 2811 |
| BspEI | TCCGGA | 1 cut(s) | 2766 |
| BspLI | GGNNCC | 1 cut(s) | 1615 |
| BspMI | ACCTGC | 1 cut(s) | 55 |
| BspPI | GGATC | 4 cut(s) | 558, 1608, 1621, 1632 |
| BspTI | CTTAAG | 1 cut(s) | 2297 |
| BspTNI | GGTCTC | 2 cut(s) | 2246, 2411 |
| BsrBI | CCGCTC | 1 cut(s) | 2311 |
| BsrDI | GCAATG | 3 cut(s) | 91, 117, 1228 |
| BsrI | ACTGG | 8 cut(s) | 1805, 1882, 2061, 2067, 2164, 2174, 2392, 2652 |
| BssECI | CCNNGG | 8 cut(s) | 597, 709, 1432, 1666, 2247, 2286, 2712, 2785 |
| BssMI | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| BssT1I | CCWWGG | 6 cut(s) | 1432, 1666, 2247, 2286, 2712, 2785 |
| Bst2UI | CCWGG | 2 cut(s) | 599, 2669 |
| Bst6I | CTCTTC | 2 cut(s) | 1710, 1959 |
| BstAFI | CTTAAG | 1 cut(s) | 2297 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1679 |
| BstC8I | GCNNGC | 3 cut(s) | 1100, 1180, 1684 |
| BstDSI | CCRYGG | 2 cut(s) | 709, 2286 |
| BstF5I | GGATG | 8 cut(s) | 100, 430, 1594, 1816, 2128, 2148, 2656, 2713 |
| BstHHI | GCGC | 1 cut(s) | 760 |
| BstKTI | GATC | 8 cut(s) | 256, 553, 738, 904, 1616, 1640, 1890, 2296 |
| BstMAI | GTCTC | 7 cut(s) | 1209, 1783, 1952, 1986, 2246, 2378, 2411 |
| BstMBI | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 458, 757, 821, 830, 1541, 1679, 2308, 2642 |
| BstNI | CCWGG | 2 cut(s) | 599, 2669 |
| BstNSI | RCATGY | 2 cut(s) | 1686, 1936 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2390 |
| BstSCI | CCNGG | 2 cut(s) | 597, 2667 |
| BstSFI | CTRYAG | 3 cut(s) | 314, 540, 2097 |
| BstSLI | GKGCMC | 3 cut(s) | 598, 973, 1360 |
| BstV1I | GCAGC | 6 cut(s) | 820, 823, 843, 1558, 1773, 2684 |
| BstV2I | GAAGAC | 4 cut(s) | 204, 915, 1010, 1512 |
| BstX2I | RGATCY | 2 cut(s) | 1613, 1637 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2159 |
| BstYI | RGATCY | 2 cut(s) | 1613, 1637 |
| BsuRI | GGCC | 1 cut(s) | 2314 |
| BtgI | CCRYGG | 2 cut(s) | 709, 2286 |
| BtrI | CACGTC | 2 cut(s) | 91, 961 |
| BtsCI | GGATG | 8 cut(s) | 100, 430, 1594, 1816, 2128, 2148, 2656, 2713 |
| BtsI | GCAGTG | 1 cut(s) | 2631 |
| BveI | ACCTGC | 1 cut(s) | 55 |
| Cac8I | GCNNGC | 3 cut(s) | 1100, 1180, 1684 |
| CfoI | GCGC | 1 cut(s) | 760 |
| Cfr13I | GGNCC | 2 cut(s) | 1720, 2520 |
| Csp6I | GTAC | 3 cut(s) | 727, 1190, 2439 |
| CviQI | GTAC | 3 cut(s) | 727, 1190, 2439 |
| DpnI | GATC | 8 cut(s) | 255, 552, 737, 903, 1615, 1639, 1889, 2295 |
| DpnII | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| DraI | TTTAAA | 2 cut(s) | 2031, 2088 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 206 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 206 |
| EaeI | YGGCCR | 1 cut(s) | 2312 |
| Eam1104I | CTCTTC | 2 cut(s) | 1710, 1959 |
| EarI | CTCTTC | 2 cut(s) | 1710, 1959 |
| Eco130I | CCWWGG | 6 cut(s) | 1432, 1666, 2247, 2286, 2712, 2785 |
| Eco24I | GRGCYC | 1 cut(s) | 393 |
| Eco31I | GGTCTC | 2 cut(s) | 2246, 2411 |
| Eco47I | GGWCC | 2 cut(s) | 1720, 2520 |
| Eco57I | CTGAAG | 2 cut(s) | 216, 1416 |
| Eco88I | CYCGRG | 1 cut(s) | 77 |
| EcoRII | CCWGG | 2 cut(s) | 597, 2667 |
| EcoT14I | CCWWGG | 6 cut(s) | 1432, 1666, 2247, 2286, 2712, 2785 |
| EcoT22I | ATGCAT | 1 cut(s) | 574 |
| EcoT38I | GRGCYC | 1 cut(s) | 393 |
| ErhI | CCWWGG | 6 cut(s) | 1432, 1666, 2247, 2286, 2712, 2785 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2240, 2272 |
| FaqI | GGGAC | 2 cut(s) | 55, 805 |
| FauNDI | CATATG | 1 cut(s) | 2191 |
| FbaI | TGATCA | 1 cut(s) | 253 |
| Fnu4HI | GCNGC | 8 cut(s) | 834, 837, 857, 1572, 1787, 2312, 2673, 2758 |
| FokI | GGATG | 8 cut(s) | 107, 417, 1581, 1803, 2135, 2135, 2663, 2720 |
| FriOI | GRGCYC | 1 cut(s) | 393 |
| Fsp4HI | GCNGC | 8 cut(s) | 834, 837, 857, 1572, 1787, 2312, 2673, 2758 |
| FspBI | CTAG | 5 cut(s) | 978, 1010, 1103, 2330, 2343 |
| GlaI | GCGC | 1 cut(s) | 759 |
| GluI | GCNGC | 8 cut(s) | 834, 837, 857, 1572, 1787, 2312, 2673, 2758 |
| GsaI | CCCAGC | 1 cut(s) | 792 |
| GsuI | CTGGAG | 2 cut(s) | 1788, 2409 |
| HaeIII | GGCC | 1 cut(s) | 2314 |
| HapII | CCGG | 1 cut(s) | 2767 |
| HhaI | GCGC | 1 cut(s) | 760 |
| Hin6I | GCGC | 1 cut(s) | 758 |
| HinP1I | GCGC | 1 cut(s) | 758 |
| HincII | GTYRAC | 3 cut(s) | 1930, 2379, 2563 |
| HindII | GTYRAC | 3 cut(s) | 1930, 2379, 2563 |
| HindIII | AAGCTT | 5 cut(s) | 887, 1697, 2135, 2300, 2750 |
| HinfI | GANTC | 9 cut(s) | 71, 532, 1087, 1478, 1832, 2145, 2339, 2391, 2664 |
| HpaII | CCGG | 1 cut(s) | 2767 |
| HphI | GGTGA | 9 cut(s) | 155, 665, 1127, 1667, 1892, 2221, 2303, 2666, 2728 |
| Hpy166II | GTNNAC | 4 cut(s) | 28, 1930, 2379, 2563 |
| Hpy8I | GTNNAC | 4 cut(s) | 28, 1930, 2379, 2563 |
| HpyAV | CCTTC | 7 cut(s) | 201, 411, 712, 771, 2035, 2578, 2824 |
| HpyCH4IV | ACGT | 5 cut(s) | 90, 149, 604, 960, 2092 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 458, 757, 821, 830, 1541, 1679, 2308, 2642 |
| HpySE526I | ACGT | 5 cut(s) | 90, 149, 604, 960, 2092 |
| HspAI | GCGC | 1 cut(s) | 758 |
| Kpn2I | TCCGGA | 1 cut(s) | 2766 |
| Ksp22I | TGATCA | 1 cut(s) | 253 |
| Kzo9I | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| LmnI | GCTCC | 7 cut(s) | 421, 493, 820, 1670, 2338, 2510, 2775 |
| Lsp1109I | GCAGC | 6 cut(s) | 820, 823, 843, 1558, 1773, 2684 |
| MaeI | CTAG | 5 cut(s) | 978, 1010, 1103, 2330, 2343 |
| MaeII | ACGT | 5 cut(s) | 90, 149, 604, 960, 2092 |
| MaeIII | GTNAC | 7 cut(s) | 150, 343, 872, 1386, 1655, 2194, 2227 |
| MalI | GATC | 8 cut(s) | 255, 552, 737, 903, 1615, 1639, 1889, 2295 |
| MbiI | CCGCTC | 1 cut(s) | 2311 |
| MboI | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| MfeI | CAATTG | 3 cut(s) | 51, 171, 1821 |
| MflI | RGATCY | 2 cut(s) | 1613, 1637 |
| MhlI | GDGCHC | 5 cut(s) | 393, 598, 973, 1184, 1360 |
| MlyI | GAGTC | 2 cut(s) | 1081, 2400 |
| MmeI | TCCRAC | 2 cut(s) | 1747, 2176 |
| Mph1103I | ATGCAT | 1 cut(s) | 574 |
| MroI | TCCGGA | 1 cut(s) | 2766 |
| MslI | CAYNNNNRTG | 5 cut(s) | 324, 959, 1152, 1514, 2208 |
| MspA1I | CMGCKG | 2 cut(s) | 856, 1235 |
| MspCI | CTTAAG | 1 cut(s) | 2297 |
| MspI | CCGG | 1 cut(s) | 2767 |
| MspR9I | CCNGG | 2 cut(s) | 599, 2669 |
| MunI | CAATTG | 3 cut(s) | 51, 171, 1821 |
| Mva1269I | GAATGC | 2 cut(s) | 574, 760 |
| MvaI | CCWGG | 2 cut(s) | 599, 2669 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 458, 757, 821, 830, 1541, 1679, 2308, 2642 |
| NcoI | CCATGG | 1 cut(s) | 2286 |
| NdeI | CATATG | 1 cut(s) | 2191 |
| NdeII | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| NlaIV | GGNNCC | 1 cut(s) | 1615 |
| NmuCI | GTSAC | 5 cut(s) | 872, 1386, 1655, 2194, 2227 |
| NsiI | ATGCAT | 1 cut(s) | 574 |
| NspI | RCATGY | 2 cut(s) | 1686, 1936 |
| PaeI | GCATGC | 1 cut(s) | 1686 |
| PaeR7I | CTCGAG | 1 cut(s) | 77 |
| PaqCI | CACCTGC | 1 cut(s) | 55 |
| PctI | GAATGC | 2 cut(s) | 574, 760 |
| PfeI | GAWTC | 7 cut(s) | 71, 532, 1478, 1832, 2145, 2339, 2664 |
| PflFI | GACNNNGTC | 1 cut(s) | 1516 |
| PkrI | GCNGC | 8 cut(s) | 835, 838, 858, 1573, 1788, 2313, 2674, 2759 |
| PleI | GAGTC | 2 cut(s) | 1081, 2399 |
| PpsI | GAGTC | 2 cut(s) | 1081, 2399 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2390 |
| Psp1406I | AACGTT | 1 cut(s) | 149 |
| Psp6I | CCWGG | 2 cut(s) | 597, 2667 |
| PspFI | CCCAGC | 1 cut(s) | 788 |
| PspGI | CCWGG | 2 cut(s) | 597, 2667 |
| PspN4I | GGNNCC | 1 cut(s) | 1615 |
| PspPI | GGNCC | 2 cut(s) | 1720, 2520 |
| PsuI | RGATCY | 2 cut(s) | 1613, 1637 |
| PsyI | GACNNNGTC | 1 cut(s) | 1516 |
| PvuII | CAGCTG | 1 cut(s) | 856 |
| RsaI | GTAC | 3 cut(s) | 728, 1191, 2440 |
| RsaNI | GTAC | 3 cut(s) | 727, 1190, 2439 |
| RseI | CAYNNNNRTG | 5 cut(s) | 324, 959, 1152, 1514, 2208 |
| SatI | GCNGC | 8 cut(s) | 834, 837, 857, 1572, 1787, 2312, 2673, 2758 |
| Sau3AI | GATC | 8 cut(s) | 253, 550, 735, 901, 1613, 1637, 1887, 2293 |
| Sau96I | GGNCC | 2 cut(s) | 1720, 2520 |
| SchI | GAGTC | 2 cut(s) | 1081, 2400 |
| ScrFI | CCNGG | 2 cut(s) | 599, 2669 |
| SduI | GDGCHC | 5 cut(s) | 393, 598, 973, 1184, 1360 |
| SfcI | CTRYAG | 3 cut(s) | 314, 540, 2097 |
| Sfr274I | CTCGAG | 1 cut(s) | 77 |
| SinI | GGWCC | 2 cut(s) | 1720, 2520 |
| SlaI | CTCGAG | 1 cut(s) | 77 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 324, 959, 1152, 1514, 2208 |
| SmlI | CTYRAG | 4 cut(s) | 77, 1633, 2297, 2822 |
| SmoI | CTYRAG | 4 cut(s) | 77, 1633, 2297, 2822 |
| SphI | GCATGC | 1 cut(s) | 1686 |
| SsiI | CCGC | 5 cut(s) | 770, 1233, 2311, 2661, 2757 |
| SspI | AATATT | 1 cut(s) | 1379 |
| SspMI | CTAG | 5 cut(s) | 978, 1010, 1103, 2330, 2343 |
| StyD4I | CCNGG | 2 cut(s) | 597, 2667 |
| StyI | CCWWGG | 6 cut(s) | 1432, 1666, 2247, 2286, 2712, 2785 |
| TaiI | ACGT | 5 cut(s) | 93, 152, 607, 963, 2095 |
| TaqI | TCGA | 5 cut(s) | 78, 904, 2143, 2242, 2423 |
| TaqII | GACCGA | 1 cut(s) | 926 |
| TauI | GCSGC | 2 cut(s) | 2314, 2760 |
| TfiI | GAWTC | 7 cut(s) | 71, 532, 1478, 1832, 2145, 2339, 2664 |
| TseFI | GTSAC | 5 cut(s) | 872, 1386, 1655, 2194, 2227 |
| TseI | GCWGC | 6 cut(s) | 833, 836, 856, 1571, 1786, 2672 |
| Tsp45I | GTSAC | 5 cut(s) | 872, 1386, 1655, 2194, 2227 |
| TspDTI | ATGAA | 9 cut(s) | 75, 128, 795, 943, 1136, 2192, 2453, 2621, 2636 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1516 |
| Vha464I | CTTAAG | 1 cut(s) | 2297 |
| VpaK11BI | GGWCC | 2 cut(s) | 1720, 2520 |
| XapI | RAATTY | 3 cut(s) | 298, 415, 2588 |
| XceI | RCATGY | 2 cut(s) | 1686, 1936 |
| XhoI | CTCGAG | 1 cut(s) | 77 |
| XspI | CTAG | 5 cut(s) | 978, 1010, 1103, 2330, 2343 |
| Zsp2I | ATGCAT | 1 cut(s) | 574 |