The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AjnI | CCWGG | 1 cut(s) | 146 |
| AluBI | AGCT | 1 cut(s) | 269 |
| AluI | AGCT | 1 cut(s) | 269 |
| Alw26I | GTCTC | 1 cut(s) | 282 |
| ApeKI | GCWGC | 1 cut(s) | 266 |
| AsuHPI | GGTGA | 1 cut(s) | 133 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 29 |
| BbvI | GCAGC | 1 cut(s) | 278 |
| BciT130I | CCWGG | 1 cut(s) | 148 |
| BclI | TGATCA | 2 cut(s) | 121, 225 |
| BcoDI | GTCTC | 1 cut(s) | 282 |
| BfmI | CTRYAG | 1 cut(s) | 236 |
| BisI | GCNGC | 1 cut(s) | 267 |
| BlsI | GCNGC | 1 cut(s) | 268 |
| Bme1390I | CCNGG | 1 cut(s) | 148 |
| BmrFI | CCNGG | 1 cut(s) | 148 |
| BsaI | GGTCTC | 1 cut(s) | 282 |
| BsaJI | CCNNGG | 2 cut(s) | 146, 147 |
| Bse1I | ACTGG | 1 cut(s) | 209 |
| BseBI | CCWGG | 1 cut(s) | 148 |
| BseDI | CCNNGG | 2 cut(s) | 146, 147 |
| BseNI | ACTGG | 1 cut(s) | 209 |
| BseXI | GCAGC | 1 cut(s) | 278 |
| BslFI | GGGAC | 1 cut(s) | 183 |
| BsmAI | GTCTC | 1 cut(s) | 282 |
| BsmFI | GGGAC | 1 cut(s) | 183 |
| Bso31I | GGTCTC | 1 cut(s) | 282 |
| Bsp143I | GATC | 2 cut(s) | 121, 225 |
| BspTNI | GGTCTC | 1 cut(s) | 282 |
| BsrI | ACTGG | 1 cut(s) | 209 |
| BssECI | CCNNGG | 2 cut(s) | 146, 147 |
| BssMI | GATC | 2 cut(s) | 121, 225 |
| Bst2UI | CCWGG | 1 cut(s) | 148 |
| Bst4CI | ACNGT | 1 cut(s) | 53 |
| BstDEI | CTNAG | 1 cut(s) | 100 |
| BstKTI | GATC | 2 cut(s) | 124, 228 |
| BstMAI | GTCTC | 1 cut(s) | 282 |
| BstMBI | GATC | 2 cut(s) | 121, 225 |
| BstNI | CCWGG | 1 cut(s) | 148 |
| BstSCI | CCNGG | 1 cut(s) | 146 |
| BstSFI | CTRYAG | 1 cut(s) | 236 |
| BstV1I | GCAGC | 1 cut(s) | 278 |
| CviAII | CATG | 1 cut(s) | 96 |
| CviJI | RGCY | 1 cut(s) | 269 |
| CviKI_1 | RGCY | 1 cut(s) | 269 |
| DdeI | CTNAG | 1 cut(s) | 100 |
| DpnI | GATC | 2 cut(s) | 123, 227 |
| DpnII | GATC | 2 cut(s) | 121, 225 |
| Eco31I | GGTCTC | 1 cut(s) | 282 |
| EcoRII | CCWGG | 1 cut(s) | 146 |
| EcoT22I | ATGCAT | 1 cut(s) | 138 |
| FaeI | CATG | 1 cut(s) | 99 |
| FaiI | YATR | 5 cut(s) | 97, 117, 134, 138, 219 |
| FaqI | GGGAC | 1 cut(s) | 183 |
| FatI | CATG | 1 cut(s) | 95 |
| FbaI | TGATCA | 2 cut(s) | 121, 225 |
| Fnu4HI | GCNGC | 1 cut(s) | 267 |
| Fsp4HI | GCNGC | 1 cut(s) | 267 |
| GluI | GCNGC | 1 cut(s) | 267 |
| Hin1II | CATG | 1 cut(s) | 99 |
| HphI | GGTGA | 1 cut(s) | 133 |
| Hpy166II | GTNNAC | 2 cut(s) | 89, 214 |
| Hpy8I | GTNNAC | 2 cut(s) | 89, 214 |
| HpyCH4III | ACNGT | 1 cut(s) | 53 |
| HpyCH4V | TGCA | 4 cut(s) | 67, 136, 158, 289 |
| HpyF3I | CTNAG | 1 cut(s) | 100 |
| Hsp92II | CATG | 1 cut(s) | 99 |
| Ksp22I | TGATCA | 2 cut(s) | 121, 225 |
| Kzo9I | GATC | 2 cut(s) | 121, 225 |
| LpnPI | CCDG | 4 cut(s) | 133, 160, 222, 224 |
| Lsp1109I | GCAGC | 1 cut(s) | 278 |
| MaeIII | GTNAC | 1 cut(s) | 12 |
| MalI | GATC | 2 cut(s) | 123, 227 |
| MboI | GATC | 2 cut(s) | 121, 225 |
| MluCI | AATT | 3 cut(s) | 46, 104, 230 |
| Mph1103I | ATGCAT | 1 cut(s) | 138 |
| MseI | TTAA | 2 cut(s) | 27, 189 |
| MslI | CAYNNNNRTG | 2 cut(s) | 94, 203 |
| MspR9I | CCNGG | 1 cut(s) | 148 |
| MvaI | CCWGG | 1 cut(s) | 148 |
| NdeII | GATC | 2 cut(s) | 121, 225 |
| NlaIII | CATG | 1 cut(s) | 99 |
| NsiI | ATGCAT | 1 cut(s) | 138 |
| PasI | CCCWGGG | 1 cut(s) | 147 |
| PkrI | GCNGC | 1 cut(s) | 268 |
| Psp6I | CCWGG | 1 cut(s) | 146 |
| PspGI | CCWGG | 1 cut(s) | 146 |
| RseI | CAYNNNNRTG | 2 cut(s) | 94, 203 |
| SaqAI | TTAA | 2 cut(s) | 27, 189 |
| SatI | GCNGC | 1 cut(s) | 267 |
| Sau3AI | GATC | 2 cut(s) | 121, 225 |
| ScrFI | CCNGG | 1 cut(s) | 148 |
| SetI | ASST | 4 cut(s) | 64, 263, 271, 278 |
| SfcI | CTRYAG | 1 cut(s) | 236 |
| SgeI | CNNG | 8 cut(s) | 44, 108, 159, 160, 221, 251, 256, 282 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 94, 203 |
| Sse9I | AATT | 3 cut(s) | 46, 104, 230 |
| StyD4I | CCNGG | 1 cut(s) | 146 |
| TaaI | ACNGT | 1 cut(s) | 53 |
| TasI | AATT | 3 cut(s) | 46, 104, 230 |
| Tru1I | TTAA | 2 cut(s) | 27, 189 |
| Tru9I | TTAA | 2 cut(s) | 27, 189 |
| TseI | GCWGC | 1 cut(s) | 266 |
| TspDTI | ATGAA | 1 cut(s) | 104 |
| Zsp2I | ATGCAT | 1 cut(s) | 138 |