Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0000969

Physical Location & Seq

Genomic Coordinates Forward (+)

69609092 .. 69615187

Gene Structure 6096 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG476800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

ATGACGGAGAATTCGTGCACGCTCCTGGTCCACTTCGACAAGGGCACGCCGGCCCTTGCCAACGAGATCAAAGAAGCTCTCGAAGGAAACGACGTCGAAGCCAAGATCGAAGCCCTCAAGCAAGCAATCATGCTCTTGCTCAACGGCGACACCATTCCCCAGCTCTTCATCACCATTATCCGCTACGTCCTTCCCTCCGAGGACCACACCGTCCAGAAGCTCCTCCTCCTCTACCTCGAGATCATTGATAAGAAAGACTCCCGAGGCAAAGTCCTACCGGAGATGATCCTCATCTGCCAGAACCTCCGGAACAATCTCCAGAGCCCTAACGAGTACATCCGCGGCGTCACGCTCCGGTTCCTCTGCCGGTTGAACGAGTCCGAGATCATCGAGCCGTTGATTCCTTCGATTCTGGCCAACCTGGAGCACCGCCACCCGTTCGTTCGACGAAACGCCGTCAGCGCAGTCATGTCTGTGTATAGGCTTCCGCACGGTGAGCAAATGCTGGTGGATGCGCCGGAGATTATCGATCGGTTCTTGGCTTCCGAGCATGATCCTTCTAGTAAGCGAAACGCGTTTCTTATGCTGTTTAATTGTGCTCAGGATAGAGCCGTCAGCTATTTGTTTTCTCATATCGATAGGATTGCTGATTGGGGCGAGCAGCTCCAGATGGTTGTGTTGGAATTGATTAGGAAGGTTTGTAGAGTCAATAAGGGTGAGAAGGGGAAGTACATTAAGGTCATTATATCATTGCTTAATGCTCAGTCCAGTGCTGTTGTTTACGAGTGTGCCAATACGCTCGTGTCCTTGTCGTCTGCCCCGACTGCTATTCGGGCCGCAGCCAATACGTATTGTCAGTTGTTATTATCACAGAGTGATAACAATGTGAAGTTGATTGTGCTCGATAGGATTAATGAGCTTAAGTCTTCGCATAGGGAGATTCTGGTTGAATTGGTTATGGATGTGCTGAGGGTACTTTCAAGTCCAAATCTTGATATTAGGAGGAAAACACTTGATATTGTTCTTGAGTTGATTACTCCGAGAAATATTGATGAGGTTGTGATGATGTTGAAGAAGGAGGTCGTCAAGACTCAGAGTGGTGAGCTTGAGAAGAATGGGGAATACAGGCAGATGCTGGTGCAAGCTATATATTCATGTGCGGTTAAGTTTCCAGAGGTGGCGAGCACAGTGGTGCATCCTTTGATGGATTTCTTGGGCGATACTAATGTGGCTTCAGCCTTGGATGTGGCTATTTTTGTGCGTGAGATAATTGAAACCAATCCCAAGTTGCGTGTTTCAATAATAATGAGGCTGTTGGACACTTTCTACCAGATTCGGACTTCCAGGGTGTGTACTTATGCTCTTTGGATTATTGGGGAGTATTGTCTCTCACTTTCTGAGGTTGAGAGTGGGATTGCAACCATTAAGCAGTGTCTTGGGGAGCTCCCATTTTACACCGCTTCTGAGGAAAGCGAGGCGCAAGATGTTTCAAAGAATTCTCAGGTTGTAAGCTCGTCGACTGTCTCTTCCAGAAGACCTGTGGTTCTTGCAGATGGGACCTATGCTACCCAGTCTGCTGCACTGGAAACTGCTATGTCTGCACCTCTCCTTGTTCAAGGATCATTGGTGGCTTCTGTAGGGAACTTGAGATCGTTGATTCTCTCTGGTGACTTTTTCCTCGAGTCAGTTGTGGCATGCACGCTGACAAAGCTTGTTTTGAGATTAGAAGAGGTTCAGCCTTCAAAAGTCGAAGTGAACAAGGCAACTACACAAGCTTTGTTAATCATGGTCTCTATGCTTCAACTGGGTCAATCATCGGCCCTTCCACATCCAATTGATAATGATTCTCATGATAGAATTGTTCTTTGCATCCGTTTACTATGCAATAAAGGTGAAGAAGTAAAGAGGATATGGTTGAAATCTTGTCGAGAGAGTTTTGTTAAAATGCTTGAAGATAAGAAGCACCGGGAAGCAGAGGAGACTAGAGCCATGGCTCAGATATCAAATGCACAGCCGGATGACCTAATTGATTTCTACCATCTAAAGAGCAAAAAGGGTATGAGCCAACTTGAGTTAGAAGATGAGGTCCAAGATGATCTCAAACGTGCCACTGGAGAGTTTCTCAAGGATGGAGATGACACAAATAAACTCAACCGCATTCTGCAGCTCACAGGATTCAGCGATCCTGTTTATGCTGAAGCCTATGTTACAGTCAATCATTATGACATTGTTCTTGATGTTACCGTCATCAATCGAACAAAGGAAACCCTTCAGAATTTGTGCTTGGAGTTGGCCACGATGGGCGATCTCAAACTCGTTGAGCGTCCTCAGAACTATACACTAGCTCCGCTATCAAGCAAACAGATAAAGTCAAACATTAAGGTCTCTTCAACTGAAACAGGAGTTATATTTGGTAACATTGTATACGAGACATCTTCAAATGTGCTTGAAAGAAACGTTATTGTACTTAATGACATCCATATTGATATAATGGATTACATCTCTCCTGCATCATGTGCTGATATATTATTCCGGACAATGTGGGCTGAGTTTGAGTGGGAGAATAAGGTACGCTTATTTGACTTTATGCTTTGTAATATCGTTCCTGTAATTAATCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005911 GO:0006810 GO:0006888 GO:0006890 GO:0006891 GO:0008150 GO:0009506 GO:0012505 GO:0012506 GO:0016020 GO:0016192 GO:0030054 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Adaptin_N Coatamer_beta_C Coatomer_b_Cpla

Protein Analysis

873

Amino Acids

98.2

Weight (kDa)

5.38

Isoelectric Point (pI)

46.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adaptin_N	PF01602	21 - 523	9.8e-93	Adaptin N terminal region
Cnd1	PF12717	113 - 265	2e-06	non-SMC mitotic condensation complex subunit 1
Coatamer_beta_C	PF07718	672 - 810	6.7e-55	Coatomer beta C-terminal region
Coatomer_b_Cpla	PF14806	817 - 859	8.8e-16	Coatomer beta subunit appendage platform

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31490 AT4G31490 AT4G31490
fragaria_vesca	FvH4_4g12700 FvH4_4g12700 FvH4_4g12700 FvH4_6g37230 FvH4_6g37230 FvH4_6g37230
malus_domestica	MD04G1023700.v1.1 MD09G1159400.v1.1 MD13G1280100.v1.1 MD17G1145600.v1.1
prunus_persica	Prupe.3G018300_v2.0.a1 Prupe.3G018400_v2.0.a1
pyrus_communis	pycom04g02030 pycom09g07780 pycom09g18330 pycom13g29300 pycom15g32330 pycom15g32340 pycom17g13850
rosa_chinensis	RchiOBHm_Chr2g0149941 RchiOBHm_Chr2g0149961 RchiOBHm_Chr2g0149991 RchiOBHm_Chr4g0411591
rosa_laevigata	RLG00000008343 RLG00000020457 RLG00000020458
rosa_multiflora	Rmu_sc0001899.1_g000002 Rmu_sc0006550.1_g000008
rosa_roxburghii	Rroxscaffold_2G00097530 Rroxscaffold_2G00097540 Rroxscaffold_5G00356170
rosa_rugosa	Rorug02G0417300 Rorug04G0107800
rosa_samantha	Rh2AG476800 Rh2AG476900 Rh2AG477100 Rh2BG488500 Rh2BG488600 Rh2CG462500 Rh2CG462600 Rh2CG462800 Rh2DG497600 Rh2DG497700 Rh4AG169200 Rh4BG166100 Rh4CG180900 Rh4DG163500
rosa_wichuraiana	Rw2G038870 Rw2G038880 Rw2G038900 Rw4G014060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	209
AatII	GACGTC	1 cut(s)	96
AccI	GTMKAC	2 cut(s)	1517, 2424
AccII	CGCG	2 cut(s)	342, 575
AccIII	TCCGGA	2 cut(s)	306, 2532
AclI	AACGTT	1 cut(s)	2457
AclWI	GGATC	4 cut(s)	280, 548, 1627, 2177
AcoI	YGGCCR	2 cut(s)	414, 2292
AcsI	RAATTY	3 cut(s)	10, 1495, 2275
AcuI	CTGAAG	3 cut(s)	1218, 2217, 2255
AcyI	GRCGYC	2 cut(s)	93, 345
AdeI	CACNNNGTG	1 cut(s)	875
AfaI	GTAC	6 cut(s)	335, 731, 975, 1354, 2466, 2571
AflII	CTTAAG	1 cut(s)	920
AflIII	ACRYGT	1 cut(s)	573
AjnI	CCWGG	3 cut(s)	24, 420, 1343
AjuI	GAANNNNNNNTTGG	2 cut(s)	2267, 2299
AleI	CACNNNNGTG	1 cut(s)	1190
Alw21I	GWGCWC	6 cut(s)	20, 429, 601, 903, 1187, 1446
Alw26I	GTCTC	6 cut(s)	1391, 1528, 1795, 1973, 2389, 2423
Alw44I	GTGCAC	1 cut(s)	16
AlwI	GGATC	4 cut(s)	280, 548, 1627, 2177
AlwNI	CAGNNNCTG	1 cut(s)	1135
Ama87I	CYCGRG	3 cut(s)	236, 261, 1679
Aor13HI	TCCGGA	2 cut(s)	306, 2532
AoxI	GGCC	5 cut(s)	51, 414, 834, 1818, 2292
ApaLI	GTGCAC	1 cut(s)	16
ApeKI	GCWGC	4 cut(s)	661, 839, 1577, 2164
ApoI	RAATTY	3 cut(s)	10, 1495, 2275
ArsI	GACNNNNNNTTYG	1 cut(s)	27
AseI	ATTAAT	2 cut(s)	912, 2613
Asp700I	GAANNNNTTC	2 cut(s)	1731, 2268
AspLEI	GCGC	3 cut(s)	464, 517, 1480
AspS9I	GGNCC	7 cut(s)	28, 52, 202, 834, 1557, 1819, 2086
AsuC2I	CCSGG	1 cut(s)	1967
AsuHPI	GGTGA	6 cut(s)	163, 506, 728, 1112, 1679, 1904
AvaI	CYCGRG	3 cut(s)	236, 261, 1679
AvaII	GGWCC	4 cut(s)	28, 202, 1557, 2086
BaeGI	GKGCMC	2 cut(s)	20, 47
BaeI	ACNNNNGTAYC	2 cut(s)	1212, 1245
BalI	TGGCCA	2 cut(s)	416, 2294
BanII	GRGCYC	2 cut(s)	326, 1446
BarI	GAAGNNNNNNTAC	2 cut(s)	713, 745
BauI	CACGAG	1 cut(s)	800
BbsI	GAAGAC	2 cut(s)	918, 1540
Bbv12I	GWGCWC	6 cut(s)	20, 429, 601, 903, 1187, 1446
BbvCI	CCTCAGC	1 cut(s)	968
BbvI	GCAGC	4 cut(s)	673, 851, 1564, 2176
BccI	CCATC	6 cut(s)	664, 1198, 1547, 2046, 2123, 2293
BceAI	ACGGC	4 cut(s)	160, 379, 440, 596
BcgI	CGANNNNNNTGC	2 cut(s)	626, 660
BciT130I	CCWGG	3 cut(s)	26, 422, 1345
BcnI	CCSGG	1 cut(s)	1967
BcoDI	GTCTC	6 cut(s)	1391, 1528, 1795, 1973, 2389, 2423
BfaI	CTAG	3 cut(s)	561, 1983, 2342
BfmI	CTRYAG	2 cut(s)	1635, 2162
BfrI	CTTAAG	1 cut(s)	920
BisI	GCNGC	6 cut(s)	343, 662, 837, 840, 1578, 2165
BlsI	GCNGC	6 cut(s)	344, 663, 838, 841, 1579, 2166
Bme1390I	CCNGG	4 cut(s)	26, 422, 1345, 1967
Bme18I	GGWCC	4 cut(s)	28, 202, 1557, 2086
BmeT110I	CYCGRG	3 cut(s)	236, 261, 1679
BmgT120I	GGNCC	7 cut(s)	28, 52, 202, 834, 1557, 1819, 2086
BmiI	GGNNCC	2 cut(s)	359, 1558
BmrFI	CCNGG	4 cut(s)	26, 422, 1345, 1967
BmrI	ACTGGG	2 cut(s)	1564, 1814
BmsI	GCATC	5 cut(s)	502, 1122, 1204, 1878, 2519
BmuI	ACTGGG	2 cut(s)	1564, 1814
BpiI	GAAGAC	2 cut(s)	918, 1540
BplI	GAGNNNNNCTC	2 cut(s)	2106, 2138
BpmI	CTGGAG	4 cut(s)	302, 443, 650, 2133
Bpu10I	CCTNAGC	2 cut(s)	600, 968
BpuEI	CTTGAG	6 cut(s)	101, 1046, 1127, 1666, 2090, 2108
BpuMI	CCSGG	1 cut(s)	1967
Bsa29I	ATCGAT	2 cut(s)	528, 636
BsaAI	YACGTR	1 cut(s)	849
BsaBI	GATNNNNATC	1 cut(s)	290
BsaHI	GRCGYC	2 cut(s)	93, 345
BsaI	GGTCTC	2 cut(s)	1795, 2389
BsaJI	CCNNGG	6 cut(s)	198, 262, 340, 1239, 1344, 1989
BsaWI	WCCGGW	4 cut(s)	277, 306, 354, 2532
BsaXI	ACNNNNNCTCC	3 cut(s)	29, 2329, 2359
Bse118I	RCCGGY	2 cut(s)	49, 366
Bse1I	ACTGG	5 cut(s)	768, 1570, 1587, 1809, 2116
Bse3DI	GCAATG	1 cut(s)	749
Bse8I	GATNNNNATC	1 cut(s)	290
BseAI	TCCGGA	2 cut(s)	306, 2532
BseBI	CCWGG	3 cut(s)	26, 422, 1345
BseCI	ATCGAT	2 cut(s)	528, 636
BseDI	CCNNGG	6 cut(s)	198, 262, 340, 1239, 1344, 1989
BseJI	GATNNNNATC	1 cut(s)	290
BseMI	GCAATG	1 cut(s)	749
BseNI	ACTGG	5 cut(s)	768, 1570, 1587, 1809, 2116
BseRI	GAGGAG	4 cut(s)	212, 215, 218, 1991
BseSI	GKGCMC	2 cut(s)	20, 47
BseXI	GCAGC	4 cut(s)	673, 851, 1564, 2176
BseYI	CCCAGC	1 cut(s)	159
BsgI	GTGCAG	2 cut(s)	1563, 1584
Bsh1236I	CGCG	2 cut(s)	342, 575
Bsh1285I	CGRYCG	1 cut(s)	532
BshFI	GGCC	5 cut(s)	53, 416, 836, 1820, 2294
BshVI	ATCGAT	2 cut(s)	528, 636
BsiEI	CGRYCG	1 cut(s)	532
BsiHKAI	GWGCWC	6 cut(s)	20, 429, 601, 903, 1187, 1446
BsiHKCI	CYCGRG	3 cut(s)	236, 261, 1679
BsiSI	CCGG	9 cut(s)	50, 278, 307, 355, 367, 518, 1966, 2015, 2533
BslFI	GGGAC	1 cut(s)	1570
BsmAI	GTCTC	6 cut(s)	1391, 1528, 1795, 1973, 2389, 2423
BsmFI	GGGAC	1 cut(s)	1570
BsmI	GAATGC	1 cut(s)	2157
BsnI	GGCC	5 cut(s)	53, 416, 836, 1820, 2294
Bso31I	GGTCTC	2 cut(s)	1795, 2389
BsoBI	CYCGRG	3 cut(s)	236, 261, 1679
Bsp1286I	GDGCHC	8 cut(s)	20, 47, 326, 429, 601, 903, 1187, 1446
Bsp13I	TCCGGA	2 cut(s)	306, 2532
Bsp19I	CCATGG	1 cut(s)	1989
BspANI	GGCC	5 cut(s)	53, 416, 836, 1820, 2294
BspDI	ATCGAT	2 cut(s)	528, 636
BspEI	TCCGGA	2 cut(s)	306, 2532
BspFNI	CGCG	2 cut(s)	342, 575
BspHI	TCATGA	1 cut(s)	1849
BspLI	GGNNCC	2 cut(s)	359, 1558
BspMAI	CTGCAG	1 cut(s)	2166
BspPI	GGATC	4 cut(s)	280, 548, 1627, 2177
BspQI	GCTCTTC	1 cut(s)	170
BspTI	CTTAAG	1 cut(s)	920
BspTNI	GGTCTC	2 cut(s)	1795, 2389
BsrDI	GCAATG	1 cut(s)	749
BsrFI	RCCGGY	2 cut(s)	49, 366
BsrI	ACTGG	5 cut(s)	768, 1570, 1587, 1809, 2116
BssAI	RCCGGY	2 cut(s)	49, 366
BssECI	CCNNGG	6 cut(s)	198, 262, 340, 1239, 1344, 1989
BssNAI	GTATAC	1 cut(s)	2425
BssNI	GRCGYC	2 cut(s)	93, 345
BssSI	CACGAG	1 cut(s)	800
BssT1I	CCWWGG	2 cut(s)	1239, 1989
Bst1107I	GTATAC	1 cut(s)	2425
Bst2BI	CACGAG	1 cut(s)	800
Bst2UI	CCWGG	3 cut(s)	26, 422, 1345
Bst4CI	ACNGT	6 cut(s)	211, 494, 1189, 1522, 2212, 2245
Bst6I	CTCTTC	4 cut(s)	170, 1531, 1722, 2392
BstACI	GRCGYC	2 cut(s)	93, 345
BstAFI	CTTAAG	1 cut(s)	920
BstAPI	GCANNNNNTGC	1 cut(s)	2516
BstBAI	YACGTR	1 cut(s)	849
BstC8I	GCNNGC	9 cut(s)	20, 47, 51, 123, 659, 1143, 1183, 1696, 1700
BstDSI	CCRYGG	2 cut(s)	340, 1989
BstFNI	CGCG	2 cut(s)	342, 575
BstHHI	GCGC	3 cut(s)	464, 517, 1480
BstMAI	GTCTC	6 cut(s)	1391, 1528, 1795, 1973, 2389, 2423
BstMCI	CGRYCG	1 cut(s)	532
BstMWI	GCNNNNNNNGC	7 cut(s)	461, 496, 824, 833, 1598, 1708, 2516
BstNI	CCWGG	3 cut(s)	26, 422, 1345
BstNSI	RCATGY	1 cut(s)	1698
BstSCI	CCNGG	4 cut(s)	24, 420, 1343, 1965
BstSFI	CTRYAG	2 cut(s)	1635, 2162
BstSLI	GKGCMC	2 cut(s)	20, 47
BstSNI	TACGTA	1 cut(s)	849
BstUI	CGCG	2 cut(s)	342, 575
BstV1I	GCAGC	4 cut(s)	673, 851, 1564, 2176
BstV2I	GAAGAC	2 cut(s)	918, 1540
BstZ17I	GTATAC	1 cut(s)	2425
Bsu15I	ATCGAT	2 cut(s)	528, 636
BsuRI	GGCC	5 cut(s)	53, 416, 836, 1820, 2294
BsuTUI	ATCGAT	2 cut(s)	528, 636
BtgI	CCRYGG	2 cut(s)	340, 1989
BtsI	GCAGTG	1 cut(s)	1436
BtsIMutI	CAGTG	5 cut(s)	775, 1194, 1436, 1580, 2109
Cac8I	GCNNGC	9 cut(s)	20, 47, 51, 123, 659, 1143, 1183, 1696, 1700
CaiI	CAGNNNCTG	1 cut(s)	1135
CciI	TCATGA	1 cut(s)	1849
CfoI	GCGC	3 cut(s)	464, 517, 1480
Cfr10I	RCCGGY	2 cut(s)	49, 366
Cfr13I	GGNCC	7 cut(s)	28, 52, 202, 834, 1557, 1819, 2086
Cfr42I	CCGCGG	1 cut(s)	343
ClaI	ATCGAT	2 cut(s)	528, 636
CseI	GACGC	2 cut(s)	334, 2312
Csp6I	GTAC	6 cut(s)	334, 730, 974, 1353, 2465, 2570
CviAII	CATG	9 cut(s)	130, 469, 551, 1155, 1695, 1786, 1850, 1990, 2514
CviQI	GTAC	6 cut(s)	334, 730, 974, 1353, 2465, 2570
DraIII	CACNNNGTG	1 cut(s)	875
DrdI	GACNNNNNNGTC	1 cut(s)	209
DseDI	GACNNNNNNGTC	1 cut(s)	209
EaeI	YGGCCR	2 cut(s)	414, 2292
Eam1104I	CTCTTC	4 cut(s)	170, 1531, 1722, 2392
EarI	CTCTTC	4 cut(s)	170, 1531, 1722, 2392
Ecl136II	GAGCTC	1 cut(s)	1444
Eco105I	TACGTA	1 cut(s)	849
Eco130I	CCWWGG	2 cut(s)	1239, 1989
Eco24I	GRGCYC	2 cut(s)	326, 1446
Eco31I	GGTCTC	2 cut(s)	1795, 2389
Eco32I	GATATC	1 cut(s)	2001
Eco47I	GGWCC	4 cut(s)	28, 202, 1557, 2086
Eco53kI	GAGCTC	1 cut(s)	1444
Eco57I	CTGAAG	3 cut(s)	1218, 2217, 2255
Eco88I	CYCGRG	3 cut(s)	236, 261, 1679
EcoICRI	GAGCTC	1 cut(s)	1444
EcoO109I	RGGNCCY	1 cut(s)	1557
EcoRI	GAATTC	2 cut(s)	10, 1495
EcoRII	CCWGG	3 cut(s)	24, 420, 1343
EcoRV	GATATC	1 cut(s)	2001
EcoT14I	CCWWGG	2 cut(s)	1239, 1989
EcoT38I	GRGCYC	2 cut(s)	326, 1446
ErhI	CCWWGG	2 cut(s)	1239, 1989
FaeI	CATG	9 cut(s)	133, 472, 554, 1158, 1698, 1789, 1853, 1993, 2517
FalI	AAGNNNNNCTT	2 cut(s)	2558, 2590
FaqI	GGGAC	1 cut(s)	1570
FatI	CATG	9 cut(s)	129, 468, 550, 1154, 1694, 1785, 1849, 1989, 2513
FblI	GTMKAC	2 cut(s)	1517, 2424
Fnu4HI	GCNGC	6 cut(s)	343, 662, 837, 840, 1578, 2165
FriOI	GRGCYC	2 cut(s)	326, 1446
Fsp4HI	GCNGC	6 cut(s)	343, 662, 837, 840, 1578, 2165
FspBI	CTAG	3 cut(s)	561, 1983, 2342
GlaI	GCGC	3 cut(s)	463, 516, 1479
GluI	GCNGC	6 cut(s)	343, 662, 837, 840, 1578, 2165
GsaI	CCCAGC	1 cut(s)	163
GsuI	CTGGAG	4 cut(s)	302, 443, 650, 2133
HaeIII	GGCC	5 cut(s)	53, 416, 836, 1820, 2294
HapII	CCGG	9 cut(s)	50, 278, 307, 355, 367, 518, 1966, 2015, 2533
HgaI	GACGC	2 cut(s)	334, 2312
HhaI	GCGC	3 cut(s)	464, 517, 1480
Hin1I	GRCGYC	2 cut(s)	93, 345
Hin1II	CATG	9 cut(s)	133, 472, 554, 1158, 1698, 1789, 1853, 1993, 2517
Hin6I	GCGC	3 cut(s)	462, 515, 1478
HinP1I	GCGC	3 cut(s)	462, 515, 1478
HincII	GTYRAC	1 cut(s)	1518
HindII	GTYRAC	1 cut(s)	1518
HindIII	AAGCTT	2 cut(s)	1709, 1773
HpaII	CCGG	9 cut(s)	50, 278, 307, 355, 367, 518, 1966, 2015, 2533
HphI	GGTGA	6 cut(s)	163, 506, 728, 1112, 1679, 1904
Hpy166II	GTNNAC	8 cut(s)	18, 31, 781, 1353, 1518, 1756, 1877, 2425
Hpy8I	GTNNAC	8 cut(s)	18, 31, 781, 1353, 1518, 1756, 1877, 2425
Hpy99I	CGWCG	4 cut(s)	95, 98, 450, 1519
HpyCH4III	ACNGT	6 cut(s)	211, 494, 1189, 1522, 2212, 2245
HpyCH4IV	ACGT	5 cut(s)	93, 186, 848, 2104, 2457
HpyF10VI	GCNNNNNNNGC	7 cut(s)	461, 496, 824, 833, 1598, 1708, 2516
HpySE526I	ACGT	5 cut(s)	93, 186, 848, 2104, 2457
Hsp92I	GRCGYC	2 cut(s)	93, 345
Hsp92II	CATG	9 cut(s)	133, 472, 554, 1158, 1698, 1789, 1853, 1993, 2517
HspAI	GCGC	3 cut(s)	462, 515, 1478
Kpn2I	TCCGGA	2 cut(s)	306, 2532
KroI	GCCGGC	1 cut(s)	49
KroNI	GCCGGC	1 cut(s)	51
KspI	CCGCGG	1 cut(s)	343
LguI	GCTCTTC	1 cut(s)	170
LmnI	GCTCC	8 cut(s)	27, 225, 357, 424, 669, 1441, 1449, 2350
Lsp1109I	GCAGC	4 cut(s)	673, 851, 1564, 2176
LweI	GCATC	5 cut(s)	502, 1122, 1204, 1878, 2519
MaeI	CTAG	3 cut(s)	561, 1983, 2342
MaeII	ACGT	5 cut(s)	93, 186, 848, 2104, 2457
MaeIII	GTNAC	5 cut(s)	346, 1667, 2206, 2239, 2414
MfeI	CAATTG	1 cut(s)	1833
MhlI	GDGCHC	8 cut(s)	20, 47, 326, 429, 601, 903, 1187, 1446
MlsI	TGGCCA	2 cut(s)	416, 2294
MluI	ACGCGT	1 cut(s)	573
MluNI	TGGCCA	2 cut(s)	416, 2294
MlyI	GAGTC	5 cut(s)	251, 386, 714, 1084, 1691
MmeI	TCCRAC	2 cut(s)	660, 1296
Mox20I	TGGCCA	2 cut(s)	416, 2294
MroI	TCCGGA	2 cut(s)	306, 2532
MroNI	GCCGGC	1 cut(s)	49
MroXI	GAANNNNTTC	2 cut(s)	1731, 2268
MscI	TGGCCA	2 cut(s)	416, 2294
MslI	CAYNNNNRTG	2 cut(s)	473, 1190
Msp20I	TGGCCA	2 cut(s)	416, 2294
MspA1I	CMGCKG	1 cut(s)	342
MspCI	CTTAAG	1 cut(s)	920
MspI	CCGG	9 cut(s)	50, 278, 307, 355, 367, 518, 1966, 2015, 2533
MspR9I	CCNGG	4 cut(s)	26, 422, 1345, 1967
MunI	CAATTG	1 cut(s)	1833
Mva1269I	GAATGC	1 cut(s)	2157
MvaI	CCWGG	3 cut(s)	26, 422, 1345
MvnI	CGCG	2 cut(s)	342, 575
MwoI	GCNNNNNNNGC	7 cut(s)	461, 496, 824, 833, 1598, 1708, 2516
NaeI	GCCGGC	1 cut(s)	51
NciI	CCSGG	1 cut(s)	1967
NcoI	CCATGG	1 cut(s)	1989
NgoMIV	GCCGGC	1 cut(s)	49
NlaIII	CATG	9 cut(s)	133, 472, 554, 1158, 1698, 1789, 1853, 1993, 2517
NlaIV	GGNNCC	2 cut(s)	359, 1558
NmuCI	GTSAC	2 cut(s)	346, 1667
NspI	RCATGY	1 cut(s)	1698
OliI	CACNNNNGTG	1 cut(s)	1190
PaeI	GCATGC	1 cut(s)	1698
PaeR7I	CTCGAG	2 cut(s)	236, 1679
PagI	TCATGA	1 cut(s)	1849
PciSI	GCTCTTC	1 cut(s)	170
PcsI	WCGNNNNNNNCGW	3 cut(s)	11, 87, 818
PctI	GAATGC	1 cut(s)	2157
PdiI	GCCGGC	1 cut(s)	51
PdmI	GAANNNNTTC	2 cut(s)	1731, 2268
PfeI	GAWTC	7 cut(s)	400, 409, 940, 1333, 1657, 1844, 2175
PkrI	GCNGC	6 cut(s)	344, 663, 838, 841, 1579, 2166
Ple19I	CGATCG	1 cut(s)	532
PleI	GAGTC	5 cut(s)	251, 385, 713, 1084, 1690
PpsI	GAGTC	5 cut(s)	251, 385, 713, 1084, 1690
Ppu21I	YACGTR	1 cut(s)	849
PpuMI	RGGWCCY	1 cut(s)	1557
PshBI	ATTAAT	2 cut(s)	912, 2613
Psp124BI	GAGCTC	1 cut(s)	1446
Psp1406I	AACGTT	1 cut(s)	2457
Psp5II	RGGWCCY	1 cut(s)	1557
Psp6I	CCWGG	3 cut(s)	24, 420, 1343
PspFI	CCCAGC	1 cut(s)	159
PspGI	CCWGG	3 cut(s)	24, 420, 1343
PspN4I	GGNNCC	2 cut(s)	359, 1558
PspPI	GGNCC	7 cut(s)	28, 52, 202, 834, 1557, 1819, 2086
PspPPI	RGGWCCY	1 cut(s)	1557
PspXI	VCTCGAGB	1 cut(s)	1679
PstI	CTGCAG	1 cut(s)	2166
PstNI	CAGNNNCTG	1 cut(s)	1135
PvuI	CGATCG	1 cut(s)	532
RsaI	GTAC	6 cut(s)	335, 731, 975, 1354, 2466, 2571
RsaNI	GTAC	6 cut(s)	334, 730, 974, 1353, 2465, 2570
RseI	CAYNNNNRTG	2 cut(s)	473, 1190
SacI	GAGCTC	1 cut(s)	1446
SacII	CCGCGG	1 cut(s)	343
SalI	GTCGAC	1 cut(s)	1516
SapI	GCTCTTC	1 cut(s)	170
SatI	GCNGC	6 cut(s)	343, 662, 837, 840, 1578, 2165
Sau96I	GGNCC	7 cut(s)	28, 52, 202, 834, 1557, 1819, 2086
SchI	GAGTC	5 cut(s)	251, 386, 714, 1084, 1691
ScrFI	CCNGG	4 cut(s)	26, 422, 1345, 1967
SduI	GDGCHC	8 cut(s)	20, 47, 326, 429, 601, 903, 1187, 1446
SfaNI	GCATC	5 cut(s)	502, 1122, 1204, 1878, 2519
SfcI	CTRYAG	2 cut(s)	1635, 2162
Sfr274I	CTCGAG	2 cut(s)	236, 1679
Sfr303I	CCGCGG	1 cut(s)	343
SgrBI	CCGCGG	1 cut(s)	343
SinI	GGWCC	4 cut(s)	28, 202, 1557, 2086
SlaI	CTCGAG	2 cut(s)	236, 1679
SmiMI	CAYNNNNRTG	2 cut(s)	473, 1190
SmlI	CTYRAG	9 cut(s)	116, 236, 920, 1025, 1106, 1645, 1679, 2069, 2123
SmoI	CTYRAG	9 cut(s)	116, 236, 920, 1025, 1106, 1645, 1679, 2069, 2123
SnaBI	TACGTA	1 cut(s)	849
SphI	GCATGC	1 cut(s)	1698
SspI	AATATT	1 cut(s)	1048
SspMI	CTAG	3 cut(s)	561, 1983, 2342
SstI	GAGCTC	1 cut(s)	1446
StyD4I	CCNGG	4 cut(s)	24, 420, 1343, 1965
StyI	CCWWGG	2 cut(s)	1239, 1989
TaaI	ACNGT	6 cut(s)	211, 494, 1189, 1522, 2212, 2245
TaiI	ACGT	5 cut(s)	96, 189, 851, 2107, 2460
TatI	WGTACW	4 cut(s)	333, 729, 1352, 2464
TauI	GCSGC	2 cut(s)	345, 839
TfiI	GAWTC	7 cut(s)	400, 409, 940, 1333, 1657, 1844, 2175
TscAI	CASTG	5 cut(s)	775, 1194, 1436, 1587, 2116
TseFI	GTSAC	2 cut(s)	346, 1667
TseI	GCWGC	4 cut(s)	661, 839, 1577, 2164
Tsp45I	GTSAC	2 cut(s)	346, 1667
TspDTI	ATGAA	2 cut(s)	157, 1143
TspGWI	ACGGA	2 cut(s)	20, 1862
TspRI	CASTG	5 cut(s)	775, 1194, 1436, 1587, 2116
Vha464I	CTTAAG	1 cut(s)	920
VneI	GTGCAC	1 cut(s)	16
VpaK11BI	GGWCC	4 cut(s)	28, 202, 1557, 2086
VspI	ATTAAT	2 cut(s)	912, 2613
XapI	RAATTY	3 cut(s)	10, 1495, 2275
XceI	RCATGY	1 cut(s)	1698
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1537
XhoI	CTCGAG	2 cut(s)	236, 1679
XmiI	GTMKAC	2 cut(s)	1517, 2424
XmnI	GAANNNNTTC	2 cut(s)	1731, 2268
XspI	CTAG	3 cut(s)	561, 1983, 2342
ZraI	GACGTC	1 cut(s)	94

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.