The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 150 |
| AccB1I | GGYRCC | 1 cut(s) | 150 |
| AclWI | GGATC | 3 cut(s) | 330, 343, 354 |
| AcsI | RAATTY | 1 cut(s) | 119 |
| AfaI | GTAC | 1 cut(s) | 152 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 84 |
| AgsI | TTSAA | 1 cut(s) | 332 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 344, 376 |
| AluBI | AGCT | 1 cut(s) | 31 |
| AluI | AGCT | 1 cut(s) | 31 |
| AlwI | GGATC | 3 cut(s) | 330, 343, 354 |
| AoxI | GGCC | 1 cut(s) | 79 |
| ApeKI | GCWGC | 2 cut(s) | 95, 293 |
| ApoI | RAATTY | 1 cut(s) | 119 |
| Asp718I | GGTACC | 1 cut(s) | 150 |
| AsuHPI | GGTGA | 1 cut(s) | 389 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 142, 175 |
| BamHI | GGATCC | 1 cut(s) | 335 |
| BanI | GGYRCC | 1 cut(s) | 150 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 187, 219 |
| BbvI | GCAGC | 2 cut(s) | 107, 280 |
| BccI | CCATC | 1 cut(s) | 263 |
| BfaI | CTAG | 2 cut(s) | 5, 300 |
| BisI | GCNGC | 2 cut(s) | 96, 294 |
| BlsI | GCNGC | 2 cut(s) | 97, 295 |
| BmiI | GGNNCC | 2 cut(s) | 152, 337 |
| BmsI | GCATC | 2 cut(s) | 146, 305 |
| BpuEI | CTTGAG | 1 cut(s) | 376 |
| BsaJI | CCNNGG | 2 cut(s) | 339, 388 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 84 |
| Bse1I | ACTGG | 1 cut(s) | 379 |
| BseDI | CCNNGG | 2 cut(s) | 339, 388 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 84 |
| BseMII | CTCAG | 2 cut(s) | 189, 419 |
| BseNI | ACTGG | 1 cut(s) | 379 |
| BseRI | GAGGAG | 1 cut(s) | 306 |
| BseXI | GCAGC | 2 cut(s) | 107, 280 |
| BshFI | GGCC | 1 cut(s) | 81 |
| BshNI | GGYRCC | 1 cut(s) | 150 |
| BsiSI | CCGG | 1 cut(s) | 78 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 84 |
| BsnI | GGCC | 1 cut(s) | 81 |
| Bsp143I | GATC | 2 cut(s) | 335, 359 |
| BspANI | GGCC | 1 cut(s) | 81 |
| BspCNI | CTCAG | 2 cut(s) | 188, 420 |
| BspHI | TCATGA | 1 cut(s) | 231 |
| BspLI | GGNNCC | 2 cut(s) | 152, 337 |
| BspPI | GGATC | 3 cut(s) | 330, 343, 354 |
| BspT107I | GGYRCC | 1 cut(s) | 150 |
| BsrI | ACTGG | 1 cut(s) | 379 |
| BssECI | CCNNGG | 2 cut(s) | 339, 388 |
| BssMI | GATC | 2 cut(s) | 335, 359 |
| BssT1I | CCWWGG | 1 cut(s) | 388 |
| Bst4CI | ACNGT | 1 cut(s) | 150 |
| BstDEI | CTNAG | 3 cut(s) | 155, 175, 428 |
| BstDSI | CCRYGG | 1 cut(s) | 339 |
| BstKTI | GATC | 2 cut(s) | 338, 362 |
| BstMBI | GATC | 2 cut(s) | 335, 359 |
| BstNSI | RCATGY | 2 cut(s) | 102, 348 |
| BstV1I | GCAGC | 2 cut(s) | 107, 280 |
| BstX2I | RGATCY | 2 cut(s) | 335, 359 |
| BstYI | RGATCY | 2 cut(s) | 335, 359 |
| BsuRI | GGCC | 1 cut(s) | 81 |
| BtgI | CCRYGG | 1 cut(s) | 339 |
| BtsIMutI | CAGTG | 1 cut(s) | 372 |
| CciI | TCATGA | 1 cut(s) | 231 |
| Csp6I | GTAC | 1 cut(s) | 151 |
| CviAII | CATG | 5 cut(s) | 99, 232, 238, 290, 345 |
| CviJI | RGCY | 2 cut(s) | 31, 81 |
| CviKI_1 | RGCY | 2 cut(s) | 31, 81 |
| CviQI | GTAC | 1 cut(s) | 151 |
| DdeI | CTNAG | 3 cut(s) | 155, 175, 428 |
| DpnI | GATC | 2 cut(s) | 337, 361 |
| DpnII | GATC | 2 cut(s) | 335, 359 |
| DraI | TTTAAA | 1 cut(s) | 247 |
| Eco130I | CCWWGG | 1 cut(s) | 388 |
| EcoT14I | CCWWGG | 1 cut(s) | 388 |
| ErhI | CCWWGG | 1 cut(s) | 388 |
| FaeI | CATG | 5 cut(s) | 102, 235, 241, 293, 348 |
| FaiI | YATR | 9 cut(s) | 20, 100, 233, 239, 279, 291, 346, 406, 413 |
| FatI | CATG | 5 cut(s) | 98, 231, 237, 289, 344 |
| Fnu4HI | GCNGC | 2 cut(s) | 96, 294 |
| Fsp4HI | GCNGC | 2 cut(s) | 96, 294 |
| FspBI | CTAG | 2 cut(s) | 5, 300 |
| GluI | GCNGC | 2 cut(s) | 96, 294 |
| HaeIII | GGCC | 1 cut(s) | 81 |
| HapII | CCGG | 1 cut(s) | 78 |
| Hin1II | CATG | 5 cut(s) | 102, 235, 241, 293, 348 |
| HinfI | GANTC | 1 cut(s) | 200 |
| HpaII | CCGG | 1 cut(s) | 78 |
| HphI | GGTGA | 1 cut(s) | 389 |
| Hpy166II | GTNNAC | 1 cut(s) | 226 |
| Hpy188I | TCNGA | 2 cut(s) | 56, 184 |
| Hpy188III | TCNNGA | 2 cut(s) | 232, 300 |
| Hpy8I | GTNNAC | 1 cut(s) | 226 |
| HpyAV | CCTTC | 1 cut(s) | 431 |
| HpyCH4III | ACNGT | 1 cut(s) | 150 |
| HpyCH4IV | ACGT | 1 cut(s) | 107 |
| HpyCH4V | TGCA | 6 cut(s) | 95, 105, 137, 166, 296, 404 |
| HpyF3I | CTNAG | 3 cut(s) | 155, 175, 428 |
| HpySE526I | ACGT | 1 cut(s) | 107 |
| Hsp92II | CATG | 5 cut(s) | 102, 235, 241, 293, 348 |
| KpnI | GGTACC | 1 cut(s) | 154 |
| Kzo9I | GATC | 2 cut(s) | 335, 359 |
| LpnPI | CCDG | 4 cut(s) | 91, 360, 377, 440 |
| Lsp1109I | GCAGC | 2 cut(s) | 107, 280 |
| LweI | GCATC | 2 cut(s) | 146, 305 |
| MaeI | CTAG | 2 cut(s) | 5, 300 |
| MaeII | ACGT | 1 cut(s) | 107 |
| MaeIII | GTNAC | 2 cut(s) | 108, 377 |
| MalI | GATC | 2 cut(s) | 337, 361 |
| MboI | GATC | 2 cut(s) | 335, 359 |
| MboII | GAAGA | 4 cut(s) | 66, 195, 197, 200 |
| MflI | RGATCY | 2 cut(s) | 335, 359 |
| MluCI | AATT | 4 cut(s) | 119, 141, 161, 303 |
| MnlI | CCTC | 1 cut(s) | 327 |
| MseI | TTAA | 3 cut(s) | 123, 144, 246 |
| MslI | CAYNNNNRTG | 1 cut(s) | 236 |
| MspI | CCGG | 1 cut(s) | 78 |
| NdeII | GATC | 2 cut(s) | 335, 359 |
| NlaIII | CATG | 5 cut(s) | 102, 235, 241, 293, 348 |
| NlaIV | GGNNCC | 2 cut(s) | 152, 337 |
| NmuCI | GTSAC | 1 cut(s) | 377 |
| NspI | RCATGY | 2 cut(s) | 102, 348 |
| PagI | TCATGA | 1 cut(s) | 231 |
| PfeI | GAWTC | 1 cut(s) | 200 |
| PflFI | GACNNNGTC | 1 cut(s) | 238 |
| PkrI | GCNGC | 2 cut(s) | 97, 295 |
| PspN4I | GGNNCC | 2 cut(s) | 152, 337 |
| PsuI | RGATCY | 2 cut(s) | 335, 359 |
| PsyI | GACNNNGTC | 1 cut(s) | 238 |
| RsaI | GTAC | 1 cut(s) | 152 |
| RsaNI | GTAC | 1 cut(s) | 151 |
| RseI | CAYNNNNRTG | 1 cut(s) | 236 |
| SaqAI | TTAA | 3 cut(s) | 123, 144, 246 |
| SatI | GCNGC | 2 cut(s) | 96, 294 |
| Sau3AI | GATC | 2 cut(s) | 335, 359 |
| SetI | ASST | 7 cut(s) | 15, 33, 110, 156, 216, 256, 411 |
| SfaNI | GCATC | 2 cut(s) | 146, 305 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 236 |
| SmlI | CTYRAG | 1 cut(s) | 355 |
| SmoI | CTYRAG | 1 cut(s) | 355 |
| Sse9I | AATT | 4 cut(s) | 119, 141, 161, 303 |
| SspMI | CTAG | 2 cut(s) | 5, 300 |
| StyI | CCWWGG | 1 cut(s) | 388 |
| TaaI | ACNGT | 1 cut(s) | 150 |
| TaiI | ACGT | 1 cut(s) | 110 |
| TasI | AATT | 4 cut(s) | 119, 141, 161, 303 |
| TfiI | GAWTC | 1 cut(s) | 200 |
| Tru1I | TTAA | 3 cut(s) | 123, 144, 246 |
| Tru9I | TTAA | 3 cut(s) | 123, 144, 246 |
| TscAI | CASTG | 1 cut(s) | 379 |
| TseFI | GTSAC | 1 cut(s) | 377 |
| TseI | GCWGC | 2 cut(s) | 95, 293 |
| Tsp45I | GTSAC | 1 cut(s) | 377 |
| TspDTI | ATGAA | 1 cut(s) | 220 |
| TspGWI | ACGGA | 1 cut(s) | 328 |
| TspRI | CASTG | 1 cut(s) | 379 |
| Tth111I | GACNNNGTC | 1 cut(s) | 238 |
| XapI | RAATTY | 1 cut(s) | 119 |
| XbaI | TCTAGA | 1 cut(s) | 299 |
| XceI | RCATGY | 2 cut(s) | 102, 348 |
| XspI | CTAG | 2 cut(s) | 5, 300 |