The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 206 |
| AatII | GACGTC | 2 cut(s) | 93, 2429 |
| AccI | GTMKAC | 1 cut(s) | 1514 |
| AccII | CGCG | 2 cut(s) | 339, 596 |
| AciI | CCGC | 9 cut(s) | 178, 304, 337, 339, 427, 485, 1455, 2149, 2841 |
| AclWI | GGATC | 3 cut(s) | 277, 1624, 2171 |
| AcoI | YGGCCR | 1 cut(s) | 2286 |
| AcsI | RAATTY | 2 cut(s) | 1492, 2269 |
| AcuI | CTGAAG | 3 cut(s) | 2141, 2211, 2249 |
| AcyI | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| AfaI | GTAC | 5 cut(s) | 332, 728, 1351, 1870, 2572 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 196, 265, 1612, 2254 |
| AflII | CTTAAG | 2 cut(s) | 917, 2036 |
| AgeI | ACCGGT | 1 cut(s) | 1810 |
| AgsI | TTSAA | 9 cut(s) | 370, 1069, 1271, 1341, 1745, 1946, 2084, 2546, 2830 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1538 |
| AjnI | CCWGG | 4 cut(s) | 21, 1036, 1526, 1671 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2261, 2293 |
| Alw21I | GWGCWC | 4 cut(s) | 426, 966, 1184, 1443 |
| Alw26I | GTCTC | 5 cut(s) | 1388, 1525, 1708, 2383, 2417 |
| AlwI | GGATC | 3 cut(s) | 277, 1624, 2171 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1132 |
| Ama87I | CYCGRG | 2 cut(s) | 233, 258 |
| AoxI | GGCC | 6 cut(s) | 48, 375, 537, 1227, 2286, 2842 |
| ApeKI | GCWGC | 5 cut(s) | 658, 833, 836, 1574, 2678 |
| ApoI | RAATTY | 2 cut(s) | 1492, 2269 |
| AseI | ATTAAT | 1 cut(s) | 909 |
| AsiGI | ACCGGT | 1 cut(s) | 1810 |
| AspLEI | GCGC | 5 cut(s) | 461, 514, 596, 598, 1990 |
| AspS9I | GGNCC | 8 cut(s) | 25, 49, 199, 1189, 1554, 1726, 1813, 2843 |
| AsuC2I | CCSGG | 1 cut(s) | 275 |
| AsuHPI | GGTGA | 8 cut(s) | 160, 503, 725, 1444, 1673, 1800, 2734, 2815 |
| AsuII | TTCGAA | 1 cut(s) | 1487 |
| AvaI | CYCGRG | 2 cut(s) | 233, 258 |
| AvaII | GGWCC | 6 cut(s) | 25, 199, 1189, 1554, 1726, 1813 |
| BaeGI | GKGCMC | 1 cut(s) | 44 |
| BalI | TGGCCA | 1 cut(s) | 2288 |
| BanII | GRGCYC | 2 cut(s) | 323, 1443 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 710, 742 |
| BauI | CACGAG | 1 cut(s) | 797 |
| BbsI | GAAGAC | 3 cut(s) | 1010, 1515, 1617 |
| Bbv12I | GWGCWC | 4 cut(s) | 426, 966, 1184, 1443 |
| BbvCI | CCTCAGC | 1 cut(s) | 1231 |
| BbvI | GCAGC | 5 cut(s) | 670, 820, 848, 1561, 2665 |
| BccI | CCATC | 9 cut(s) | 661, 1195, 1200, 1544, 2040, 2117, 2287, 2653, 2744 |
| BceAI | ACGGC | 3 cut(s) | 157, 376, 437 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 342, 376 |
| BciT130I | CCWGG | 4 cut(s) | 23, 1038, 1528, 1673 |
| BciVI | GTATCC | 3 cut(s) | 1210, 1309, 2759 |
| BclI | TGATCA | 1 cut(s) | 1776 |
| BcnI | CCSGG | 1 cut(s) | 275 |
| BcoDI | GTCTC | 5 cut(s) | 1388, 1525, 1708, 2383, 2417 |
| BfaI | CTAG | 2 cut(s) | 1718, 2336 |
| BfmI | CTRYAG | 4 cut(s) | 834, 1170, 1536, 1629 |
| BfrI | CTTAAG | 2 cut(s) | 917, 2036 |
| BfuI | GTATCC | 3 cut(s) | 1210, 1309, 2759 |
| BisI | GCNGC | 7 cut(s) | 340, 659, 834, 837, 1575, 2679, 2842 |
| BlpI | GCTNAGC | 2 cut(s) | 654, 1098 |
| BlsI | GCNGC | 7 cut(s) | 341, 660, 835, 838, 1576, 2680, 2843 |
| BmcAI | AGTACT | 1 cut(s) | 2572 |
| Bme1390I | CCNGG | 5 cut(s) | 23, 275, 1038, 1528, 1673 |
| Bme18I | GGWCC | 6 cut(s) | 25, 199, 1189, 1554, 1726, 1813 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1538 |
| BmeT110I | CYCGRG | 2 cut(s) | 233, 258 |
| BmgT120I | GGNCC | 8 cut(s) | 25, 49, 199, 1189, 1554, 1726, 1813, 2843 |
| BmiI | GGNNCC | 2 cut(s) | 1555, 2260 |
| BmrFI | CCNGG | 5 cut(s) | 23, 275, 1038, 1528, 1673 |
| BmrI | ACTGGG | 2 cut(s) | 1561, 1808 |
| BmsI | GCATC | 6 cut(s) | 499, 1119, 1872, 1966, 2122, 2704 |
| BmuI | ACTGGG | 2 cut(s) | 1561, 1808 |
| BpiI | GAAGAC | 3 cut(s) | 1010, 1515, 1617 |
| BpmI | CTGGAG | 6 cut(s) | 299, 440, 647, 1694, 2127, 2325 |
| Bpu10I | CCTNAGC | 2 cut(s) | 965, 1231 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 654, 1098 |
| Bpu14I | TTCGAA | 1 cut(s) | 1487 |
| BpuEI | CTTGAG | 5 cut(s) | 1124, 1660, 2084, 2459, 2775 |
| BpuMI | CCSGG | 1 cut(s) | 275 |
| Bsa29I | ATCGAT | 1 cut(s) | 633 |
| BsaBI | GATNNNNATC | 2 cut(s) | 287, 1221 |
| BsaHI | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| BsaI | GGTCTC | 2 cut(s) | 1525, 2383 |
| BsaJI | CCNNGG | 4 cut(s) | 195, 259, 337, 2253 |
| BsaWI | WCCGGW | 1 cut(s) | 1810 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2100, 2130, 2323, 2353 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 196, 265, 1612, 2254 |
| Bse118I | RCCGGY | 3 cut(s) | 46, 363, 1810 |
| Bse1I | ACTGG | 8 cut(s) | 507, 558, 765, 1567, 1584, 1803, 2110, 2658 |
| Bse3DI | GCAATG | 1 cut(s) | 746 |
| Bse8I | GATNNNNATC | 2 cut(s) | 287, 1221 |
| BseBI | CCWGG | 4 cut(s) | 23, 1038, 1528, 1673 |
| BseCI | ATCGAT | 1 cut(s) | 633 |
| BseDI | CCNNGG | 4 cut(s) | 195, 259, 337, 2253 |
| BseJI | GATNNNNATC | 2 cut(s) | 287, 1221 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 196, 265, 1612, 2254 |
| BseMI | GCAATG | 1 cut(s) | 746 |
| BseNI | ACTGG | 8 cut(s) | 507, 558, 765, 1567, 1584, 1803, 2110, 2658 |
| BsePI | GCGCGC | 1 cut(s) | 594 |
| BseRI | GAGGAG | 3 cut(s) | 212, 215, 2371 |
| BseSI | GKGCMC | 1 cut(s) | 44 |
| BseXI | GCAGC | 5 cut(s) | 670, 820, 848, 1561, 2665 |
| BseYI | CCCAGC | 1 cut(s) | 156 |
| BsgI | GTGCAG | 3 cut(s) | 1176, 1560, 1581 |
| Bsh1236I | CGCG | 2 cut(s) | 339, 596 |
| BshFI | GGCC | 6 cut(s) | 50, 377, 539, 1229, 2288, 2844 |
| BshTI | ACCGGT | 1 cut(s) | 1810 |
| BshVI | ATCGAT | 1 cut(s) | 633 |
| BsiHKAI | GWGCWC | 4 cut(s) | 426, 966, 1184, 1443 |
| BsiHKCI | CYCGRG | 2 cut(s) | 233, 258 |
| BsiSI | CCGG | 5 cut(s) | 47, 275, 364, 515, 1811 |
| BslFI | GGGAC | 2 cut(s) | 1567, 2828 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 196, 265, 1612, 2254 |
| BsmAI | GTCTC | 5 cut(s) | 1388, 1525, 1708, 2383, 2417 |
| BsmBI | CGTCTC | 1 cut(s) | 2417 |
| BsmFI | GGGAC | 2 cut(s) | 1567, 2828 |
| BsmI | GAATGC | 1 cut(s) | 2151 |
| BsnI | GGCC | 6 cut(s) | 50, 377, 539, 1229, 2288, 2844 |
| Bso31I | GGTCTC | 2 cut(s) | 1525, 2383 |
| BsoBI | CYCGRG | 2 cut(s) | 233, 258 |
| Bsp119I | TTCGAA | 1 cut(s) | 1487 |
| Bsp1286I | GDGCHC | 6 cut(s) | 44, 323, 426, 966, 1184, 1443 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 654, 1098 |
| BspACI | CCGC | 9 cut(s) | 178, 304, 337, 339, 427, 485, 1455, 2149, 2841 |
| BspANI | GGCC | 6 cut(s) | 50, 377, 539, 1229, 2288, 2844 |
| BspDI | ATCGAT | 1 cut(s) | 633 |
| BspFNI | CGCG | 2 cut(s) | 339, 596 |
| BspHI | TCATGA | 2 cut(s) | 126, 1843 |
| BspLI | GGNNCC | 2 cut(s) | 1555, 2260 |
| BspMAI | CTGCAG | 2 cut(s) | 838, 1174 |
| BspPI | GGATC | 3 cut(s) | 277, 1624, 2171 |
| BspQI | GCTCTTC | 1 cut(s) | 167 |
| BspT104I | TTCGAA | 1 cut(s) | 1487 |
| BspTI | CTTAAG | 2 cut(s) | 917, 2036 |
| BspTNI | GGTCTC | 2 cut(s) | 1525, 2383 |
| BsrDI | GCAATG | 1 cut(s) | 746 |
| BsrFI | RCCGGY | 3 cut(s) | 46, 363, 1810 |
| BsrI | ACTGG | 8 cut(s) | 507, 558, 765, 1567, 1584, 1803, 2110, 2658 |
| BssAI | RCCGGY | 3 cut(s) | 46, 363, 1810 |
| BssECI | CCNNGG | 4 cut(s) | 195, 259, 337, 2253 |
| BssHII | GCGCGC | 1 cut(s) | 594 |
| BssNI | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| BssSI | CACGAG | 1 cut(s) | 797 |
| BssT1I | CCWWGG | 1 cut(s) | 2253 |
| Bst2BI | CACGAG | 1 cut(s) | 797 |
| Bst2UI | CCWGG | 4 cut(s) | 23, 1038, 1528, 1673 |
| Bst4CI | ACNGT | 8 cut(s) | 208, 851, 1186, 1519, 2206, 2239, 2449, 2575 |
| Bst6I | CTCTTC | 1 cut(s) | 167 |
| BstACI | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| BstAFI | CTTAAG | 2 cut(s) | 917, 2036 |
| BstBI | TTCGAA | 1 cut(s) | 1487 |
| BstC8I | GCNNGC | 8 cut(s) | 17, 44, 48, 596, 1140, 1180, 1690, 2060 |
| BstDSI | CCRYGG | 1 cut(s) | 337 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1610 |
| BstFNI | CGCG | 2 cut(s) | 339, 596 |
| BstHHI | GCGC | 5 cut(s) | 461, 514, 596, 598, 1990 |
| BstMAI | GTCTC | 5 cut(s) | 1388, 1525, 1708, 2383, 2417 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 458, 493, 821, 830, 1595 |
| BstNI | CCWGG | 4 cut(s) | 23, 1038, 1528, 1673 |
| BstSCI | CCNGG | 5 cut(s) | 21, 273, 1036, 1526, 1671 |
| BstSFI | CTRYAG | 4 cut(s) | 834, 1170, 1536, 1629 |
| BstSLI | GKGCMC | 1 cut(s) | 44 |
| BstUI | CGCG | 2 cut(s) | 339, 596 |
| BstV1I | GCAGC | 5 cut(s) | 670, 820, 848, 1561, 2665 |
| BstV2I | GAAGAC | 3 cut(s) | 1010, 1515, 1617 |
| BstX2I | RGATCY | 1 cut(s) | 1616 |
| BstYI | RGATCY | 1 cut(s) | 1616 |
| Bsu15I | ATCGAT | 1 cut(s) | 633 |
| BsuI | GTATCC | 3 cut(s) | 1210, 1309, 2759 |
| BsuRI | GGCC | 6 cut(s) | 50, 377, 539, 1229, 2288, 2844 |
| BsuTUI | ATCGAT | 1 cut(s) | 633 |
| BtgI | CCRYGG | 1 cut(s) | 337 |
| BtsI | GCAGTG | 3 cut(s) | 819, 1179, 1433 |
| BtsIMutI | CAGTG | 7 cut(s) | 772, 819, 1179, 1433, 1577, 2651, 2801 |
| Cac8I | GCNNGC | 8 cut(s) | 17, 44, 48, 596, 1140, 1180, 1690, 2060 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1132 |
| CciI | TCATGA | 2 cut(s) | 126, 1843 |
| CfoI | GCGC | 5 cut(s) | 461, 514, 596, 598, 1990 |
| Cfr10I | RCCGGY | 3 cut(s) | 46, 363, 1810 |
| Cfr13I | GGNCC | 8 cut(s) | 25, 49, 199, 1189, 1554, 1726, 1813, 2843 |
| Cfr42I | CCGCGG | 1 cut(s) | 340 |
| ClaI | ATCGAT | 1 cut(s) | 633 |
| CseI | GACGC | 2 cut(s) | 331, 2306 |
| Csp6I | GTAC | 5 cut(s) | 331, 727, 1350, 1869, 2571 |
| CspAI | ACCGGT | 1 cut(s) | 1810 |
| CviAII | CATG | 9 cut(s) | 12, 112, 127, 466, 1152, 1780, 1844, 2505, 2626 |
| CviQI | GTAC | 5 cut(s) | 331, 727, 1350, 1869, 2571 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 206 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1538 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 206 |
| EaeI | YGGCCR | 1 cut(s) | 2286 |
| Eam1104I | CTCTTC | 1 cut(s) | 167 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1538 |
| EarI | CTCTTC | 1 cut(s) | 167 |
| Ecl136II | GAGCTC | 1 cut(s) | 1441 |
| Eco130I | CCWWGG | 1 cut(s) | 2253 |
| Eco24I | GRGCYC | 2 cut(s) | 323, 1443 |
| Eco31I | GGTCTC | 2 cut(s) | 1525, 2383 |
| Eco32I | GATATC | 1 cut(s) | 1995 |
| Eco47I | GGWCC | 6 cut(s) | 25, 199, 1189, 1554, 1726, 1813 |
| Eco53kI | GAGCTC | 1 cut(s) | 1441 |
| Eco57I | CTGAAG | 3 cut(s) | 2141, 2211, 2249 |
| Eco88I | CYCGRG | 2 cut(s) | 233, 258 |
| EcoICRI | GAGCTC | 1 cut(s) | 1441 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1610 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1554 |
| EcoRI | GAATTC | 1 cut(s) | 1492 |
| EcoRII | CCWGG | 4 cut(s) | 21, 1036, 1526, 1671 |
| EcoRV | GATATC | 1 cut(s) | 1995 |
| EcoT14I | CCWWGG | 1 cut(s) | 2253 |
| EcoT38I | GRGCYC | 2 cut(s) | 323, 1443 |
| ErhI | CCWWGG | 1 cut(s) | 2253 |
| Esp3I | CGTCTC | 1 cut(s) | 2417 |
| FaeI | CATG | 9 cut(s) | 15, 115, 130, 469, 1155, 1783, 1847, 2508, 2629 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2810, 2842 |
| FaqI | GGGAC | 2 cut(s) | 1567, 2828 |
| FatI | CATG | 9 cut(s) | 11, 111, 126, 465, 1151, 1779, 1843, 2504, 2625 |
| FbaI | TGATCA | 1 cut(s) | 1776 |
| FblI | GTMKAC | 1 cut(s) | 1514 |
| Fnu4HI | GCNGC | 7 cut(s) | 340, 659, 834, 837, 1575, 2679, 2842 |
| FriOI | GRGCYC | 2 cut(s) | 323, 1443 |
| Fsp4HI | GCNGC | 7 cut(s) | 340, 659, 834, 837, 1575, 2679, 2842 |
| FspBI | CTAG | 2 cut(s) | 1718, 2336 |
| GlaI | GCGC | 5 cut(s) | 460, 513, 595, 597, 1989 |
| GluI | GCNGC | 7 cut(s) | 340, 659, 834, 837, 1575, 2679, 2842 |
| GsaI | CCCAGC | 1 cut(s) | 160 |
| GsuI | CTGGAG | 6 cut(s) | 299, 440, 647, 1694, 2127, 2325 |
| HaeIII | GGCC | 6 cut(s) | 50, 377, 539, 1229, 2288, 2844 |
| HapII | CCGG | 5 cut(s) | 47, 275, 364, 515, 1811 |
| HgaI | GACGC | 2 cut(s) | 331, 2306 |
| HhaI | GCGC | 5 cut(s) | 461, 514, 596, 598, 1990 |
| Hin1I | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| Hin1II | CATG | 9 cut(s) | 15, 115, 130, 469, 1155, 1783, 1847, 2508, 2629 |
| Hin6I | GCGC | 5 cut(s) | 459, 512, 594, 596, 1988 |
| HinP1I | GCGC | 5 cut(s) | 459, 512, 594, 596, 1988 |
| HincII | GTYRAC | 1 cut(s) | 1750 |
| HindII | GTYRAC | 1 cut(s) | 1750 |
| HindIII | AAGCTT | 2 cut(s) | 215, 1703 |
| HpaI | GTTAAC | 1 cut(s) | 1750 |
| HpaII | CCGG | 5 cut(s) | 47, 275, 364, 515, 1811 |
| HphI | GGTGA | 8 cut(s) | 160, 503, 725, 1444, 1673, 1800, 2734, 2815 |
| Hpy166II | GTNNAC | 8 cut(s) | 28, 624, 778, 1350, 1507, 1515, 1750, 1871 |
| Hpy8I | GTNNAC | 8 cut(s) | 28, 624, 778, 1350, 1507, 1515, 1750, 1871 |
| Hpy99I | CGWCG | 5 cut(s) | 92, 95, 389, 406, 447 |
| HpyCH4III | ACNGT | 8 cut(s) | 208, 851, 1186, 1519, 2206, 2239, 2449, 2575 |
| HpyCH4IV | ACGT | 4 cut(s) | 90, 183, 2098, 2426 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 458, 493, 821, 830, 1595 |
| HpySE526I | ACGT | 4 cut(s) | 90, 183, 2098, 2426 |
| Hsp92I | GRCGYC | 3 cut(s) | 90, 342, 2426 |
| Hsp92II | CATG | 9 cut(s) | 15, 115, 130, 469, 1155, 1783, 1847, 2508, 2629 |
| HspAI | GCGC | 5 cut(s) | 459, 512, 594, 596, 1988 |
| KroI | GCCGGC | 1 cut(s) | 46 |
| KroNI | GCCGGC | 1 cut(s) | 48 |
| Ksp22I | TGATCA | 1 cut(s) | 1776 |
| KspAI | GTTAAC | 1 cut(s) | 1750 |
| KspI | CCGCGG | 1 cut(s) | 340 |
| LguI | GCTCTTC | 1 cut(s) | 167 |
| LmnI | GCTCC | 7 cut(s) | 24, 354, 421, 666, 1438, 1446, 2344 |
| Lsp1109I | GCAGC | 5 cut(s) | 670, 820, 848, 1561, 2665 |
| LweI | GCATC | 6 cut(s) | 499, 1119, 1872, 1966, 2122, 2704 |
| MaeI | CTAG | 2 cut(s) | 1718, 2336 |
| MaeII | ACGT | 4 cut(s) | 90, 183, 2098, 2426 |
| MaeIII | GTNAC | 6 cut(s) | 343, 864, 1661, 2200, 2233, 2408 |
| MfeI | CAATTG | 1 cut(s) | 1827 |
| MflI | RGATCY | 1 cut(s) | 1616 |
| MhlI | GDGCHC | 6 cut(s) | 44, 323, 426, 966, 1184, 1443 |
| MlsI | TGGCCA | 1 cut(s) | 2288 |
| MluNI | TGGCCA | 1 cut(s) | 2288 |
| MlyI | GAGTC | 9 cut(s) | 711, 988, 1000, 1081, 1685, 2632, 2679, 2782, 2815 |
| MmeI | TCCRAC | 3 cut(s) | 657, 1293, 2049 |
| Mox20I | TGGCCA | 1 cut(s) | 2288 |
| MroNI | GCCGGC | 1 cut(s) | 46 |
| MscI | TGGCCA | 1 cut(s) | 2288 |
| MslI | CAYNNNNRTG | 1 cut(s) | 470 |
| Msp20I | TGGCCA | 1 cut(s) | 2288 |
| MspA1I | CMGCKG | 1 cut(s) | 339 |
| MspCI | CTTAAG | 2 cut(s) | 917, 2036 |
| MspI | CCGG | 5 cut(s) | 47, 275, 364, 515, 1811 |
| MspR9I | CCNGG | 5 cut(s) | 23, 275, 1038, 1528, 1673 |
| MunI | CAATTG | 1 cut(s) | 1827 |
| Mva1269I | GAATGC | 1 cut(s) | 2151 |
| MvaI | CCWGG | 4 cut(s) | 23, 1038, 1528, 1673 |
| MvnI | CGCG | 2 cut(s) | 339, 596 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 458, 493, 821, 830, 1595 |
| NaeI | GCCGGC | 1 cut(s) | 48 |
| NciI | CCSGG | 1 cut(s) | 275 |
| NgoMIV | GCCGGC | 1 cut(s) | 46 |
| NlaIII | CATG | 9 cut(s) | 15, 115, 130, 469, 1155, 1783, 1847, 2508, 2629 |
| NlaIV | GGNNCC | 2 cut(s) | 1555, 2260 |
| NmeAIII | GCCGAG | 1 cut(s) | 403 |
| NmuCI | GTSAC | 3 cut(s) | 343, 864, 1661 |
| NspV | TTCGAA | 1 cut(s) | 1487 |
| PaeR7I | CTCGAG | 1 cut(s) | 233 |
| PagI | TCATGA | 2 cut(s) | 126, 1843 |
| PauI | GCGCGC | 1 cut(s) | 594 |
| PciSI | GCTCTTC | 1 cut(s) | 167 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 410, 2235 |
| PctI | GAATGC | 1 cut(s) | 2151 |
| PdiI | GCCGGC | 1 cut(s) | 48 |
| PfoI | TCCNGGA | 4 cut(s) | 273, 1036, 1526, 1671 |
| PinAI | ACCGGT | 1 cut(s) | 1810 |
| PkrI | GCNGC | 7 cut(s) | 341, 660, 835, 838, 1576, 2680, 2843 |
| PleI | GAGTC | 9 cut(s) | 710, 987, 1000, 1081, 1684, 2632, 2678, 2782, 2814 |
| PpsI | GAGTC | 9 cut(s) | 710, 987, 1000, 1081, 1684, 2632, 2678, 2782, 2814 |
| PpuMI | RGGWCCY | 1 cut(s) | 1554 |
| PshBI | ATTAAT | 1 cut(s) | 909 |
| Psp124BI | GAGCTC | 1 cut(s) | 1443 |
| Psp5II | RGGWCCY | 1 cut(s) | 1554 |
| Psp6I | CCWGG | 4 cut(s) | 21, 1036, 1526, 1671 |
| PspFI | CCCAGC | 1 cut(s) | 156 |
| PspGI | CCWGG | 4 cut(s) | 21, 1036, 1526, 1671 |
| PspN4I | GGNNCC | 2 cut(s) | 1555, 2260 |
| PspPI | GGNCC | 8 cut(s) | 25, 49, 199, 1189, 1554, 1726, 1813, 2843 |
| PspPPI | RGGWCCY | 1 cut(s) | 1554 |
| PstI | CTGCAG | 2 cut(s) | 838, 1174 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1132 |
| PsuI | RGATCY | 1 cut(s) | 1616 |
| PteI | GCGCGC | 1 cut(s) | 594 |
| RsaI | GTAC | 5 cut(s) | 332, 728, 1351, 1870, 2572 |
| RsaNI | GTAC | 5 cut(s) | 331, 727, 1350, 1869, 2571 |
| RseI | CAYNNNNRTG | 1 cut(s) | 470 |
| SacI | GAGCTC | 1 cut(s) | 1443 |
| SacII | CCGCGG | 1 cut(s) | 340 |
| SapI | GCTCTTC | 1 cut(s) | 167 |
| SatI | GCNGC | 7 cut(s) | 340, 659, 834, 837, 1575, 2679, 2842 |
| Sau96I | GGNCC | 8 cut(s) | 25, 49, 199, 1189, 1554, 1726, 1813, 2843 |
| ScaI | AGTACT | 1 cut(s) | 2572 |
| SchI | GAGTC | 9 cut(s) | 711, 988, 1000, 1081, 1685, 2632, 2679, 2782, 2815 |
| ScrFI | CCNGG | 5 cut(s) | 23, 275, 1038, 1528, 1673 |
| SduI | GDGCHC | 6 cut(s) | 44, 323, 426, 966, 1184, 1443 |
| SfaNI | GCATC | 6 cut(s) | 499, 1119, 1872, 1966, 2122, 2704 |
| SfcI | CTRYAG | 4 cut(s) | 834, 1170, 1536, 1629 |
| Sfr274I | CTCGAG | 1 cut(s) | 233 |
| Sfr303I | CCGCGG | 1 cut(s) | 340 |
| SfuI | TTCGAA | 1 cut(s) | 1487 |
| SgrBI | CCGCGG | 1 cut(s) | 340 |
| SinI | GGWCC | 6 cut(s) | 25, 199, 1189, 1554, 1726, 1813 |
| SlaI | CTCGAG | 1 cut(s) | 233 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 470 |
| SmlI | CTYRAG | 8 cut(s) | 233, 917, 1103, 1639, 2036, 2063, 2438, 2790 |
| SmoI | CTYRAG | 8 cut(s) | 233, 917, 1103, 1639, 2036, 2063, 2438, 2790 |
| SsiI | CCGC | 9 cut(s) | 178, 304, 337, 339, 427, 485, 1455, 2149, 2841 |
| SspI | AATATT | 1 cut(s) | 1045 |
| SspMI | CTAG | 2 cut(s) | 1718, 2336 |
| SstI | GAGCTC | 1 cut(s) | 1443 |
| StyD4I | CCNGG | 5 cut(s) | 21, 273, 1036, 1526, 1671 |
| StyI | CCWWGG | 1 cut(s) | 2253 |
| TaaI | ACNGT | 8 cut(s) | 208, 851, 1186, 1519, 2206, 2239, 2449, 2575 |
| TaiI | ACGT | 4 cut(s) | 93, 186, 2101, 2429 |
| TatI | WGTACW | 4 cut(s) | 330, 726, 1349, 2570 |
| TauI | GCSGC | 2 cut(s) | 342, 2844 |
| TscAI | CASTG | 7 cut(s) | 772, 826, 1179, 1433, 1584, 2658, 2808 |
| TseFI | GTSAC | 3 cut(s) | 343, 864, 1661 |
| TseI | GCWGC | 5 cut(s) | 658, 833, 836, 1574, 2678 |
| Tsp45I | GTSAC | 3 cut(s) | 343, 864, 1661 |
| TspDTI | ATGAA | 6 cut(s) | 128, 154, 1140, 1902, 2493, 2642 |
| TspGWI | ACGGA | 3 cut(s) | 390, 460, 1856 |
| TspRI | CASTG | 7 cut(s) | 772, 826, 1179, 1433, 1584, 2658, 2808 |
| Vha464I | CTTAAG | 2 cut(s) | 917, 2036 |
| VpaK11BI | GGWCC | 6 cut(s) | 25, 199, 1189, 1554, 1726, 1813 |
| VspI | ATTAAT | 1 cut(s) | 909 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1610 |
| XapI | RAATTY | 2 cut(s) | 1492, 2269 |
| XhoI | CTCGAG | 1 cut(s) | 233 |
| XmiI | GTMKAC | 1 cut(s) | 1514 |
| XspI | CTAG | 2 cut(s) | 1718, 2336 |
| ZraI | GACGTC | 2 cut(s) | 91, 2427 |
| ZrmI | AGTACT | 1 cut(s) | 2572 |